Publications

calcium channel blocker inhibition of the calmodulin-dependent effects of thyroid hormone and milrinone on rabbit myocardial membrane ca2+-atpase activity.	the ca2+-atpase activity of rabbit myocardial membranes is stimulated in vitro by l-thyroxine and by milrinone, a bipyridine. these effects are concentration dependent and calmodulin requiring. the calcium channel blockers nifedipine and verapamil have been reported to have anti-calmodulin effects in other assay systems. in this study we have examined the effects of nifedipine and verapamil on rabbit myocardial membrane ca2+-atpase activity, in the absence (basal activity) and presence of exogen ...	1988	2968801
treatment of locally advanced prostatic carcinoma with lhrh analogues: cytological, dna-cytophotometrical, and clinical results.	from june 1, 1981 to december 31, 1985, 122 patients aged 54 to 83 years, with locally advanced prostatic carcinoma, were treated with buserelin. nineteen of the patients received combined therapy with buserelin and androcur for the first 3 months. to control the response of the primary tumor to therapy, fine-needle aspiration biopsy of the prostate was made in all patients at 3-month intervals. fifty-eight (76.3%) of 76 patients with locally advanced prostatic carcinoma, with or without bone me ...	1988	2968759
modulation of atrial natriuretic factor by thyroid hormone: messenger ribonucleic acid and peptide levels in hypothyroid, euthyroid, and hyperthyroid rat atria and ventricles.	the effect of thyroid hormone on atrial natriuretic factor (anf) production was investigated in hypothyroid, euthyroid, and hyperthyroid rats by measuring levels of anf mrna and anf in myocardium. anf mrna was quantitated by dot blot hybridization, and anf by specific ria. relative anf mrna concentrations (anf mrna to 18s rna) were determined for right atria, left atria, and ventricular apices. the total chamber content of anf mrna was estimated (concentration x total chamber rna) and used as a ...	1988	2968239
thyroxine-induced cardiac hypertrophy: time course of development and inhibition by propranolol.	to investigate the mechanism of thyroid hormone-induced cardiac hypertrophy, we have studied the in vivo changes in cardiac size and total myocardial content of both membrane and cytoskeletal enzymes in the rat after the administration of excess thyroid hormone. in response to 50 micrograms t4/day, there is a significant increase in heart rate and heart work associated with an increase in total heart size and protein content. measurements of the specific activity of na,k-atpase and p-nitrophenol ...	1988	2968237
bacterial infections in human immunodeficiency virus-infected children.	a retrospective review of 71 children infected with human immunodeficiency virus cared for over a 3.5-year period revealed that 44 of 71 (63%) required a bacterial culture and 27 of 71 (37%) had bacteriologically documented infection. there were 125 episodes in 27 patients. pneumonia (24 of 125 (19%)), upper respiratory tract syndromes (23 of 125 (19%)), urinary tract infection (24 of 125 (19%)) and wound infection (12 of 125 (10%)) were the most common syndromes identified. bacteremic infection ...	1988	2967948
the mechanism of homologous dna strand exchange catalyzed by the bacteriophage t4 uvsx and gene 32 proteins.	a strand exchange reaction between a single-stranded dna circle and a homologous linear double-stranded dna molecule is catalyzed by a mixture of two t4 bacteriophage proteins, the uvsx protein (a dna-dependent atpase that resembles the reca protein) and the gene 32 protein (a helix-destabilizing protein). the products are different from those formed in the corresponding reca protein-catalyzed reaction; rather than producing a linear single strand plus a nicked circular double-stranded (form ii) ...	1988	2967823
role of the 2h4 molecule in the activation of suppressor inducer function.	the monoclonal antibody anti-2h4 recognizes a 220-kda and 200-kda glycoprotein and subdivides t4+ cells into distinct subpopulations: the t4+2h4+ inducer of suppression and the t4+2h4- inducer of help. the t4+2h4+ subset has been shown to play a crucial role in the activation of t8+ suppressor cells. in the present study, we attempted to determine whether the 2h4 molecule itself may be involved in initial triggering of suppressor inducer function. the results show that the addition of anti-2h4 a ...	1988	2967762
prognostic value of langerhans cells in the epidermis of hiv patients.	we measured the number of langerhans cells in clinically normal epidermis and the number of t4 lymphocytes in the plasma in 24 patients infected with hiv, and related these to the stage of the disease. a correlation is demonstrated between the numbers of langerhans cells and t4 lymphocytes. numbers of langerhans cells were lower in patients with disease stages iii and iv than in those with disease stage ii. thus langerhans cell numbers could be of prognostic value in this disease.	1988	2967714
formation of complexes between long tail fibres and substructural elements of phage t4d.	complexes of substructural elements of bacteriophage t4 (baseplates, baseplate-core complexes) with long tail fibres were obtained for the first time by complementation in vitro. a study of the organization of the complexes was carried out by page, electron microscopy and sedimentation analysis. about 90% of baseplates and baseplate-core complexes were combined with fibres. however, the number of the attached fibres varied from one to six. on the basis of the data obtained, we proposed that the ...	1988	2967347
t cell-dependent activation of b cell proliferation and differentiation by immobilized monoclonal antibodies to cd3.	the capacity of mab directed at the cd3 molecular complex (64.1) to induce t cell-dependent b cell proliferation and differentiation was examined. coculture of b cells with mitomycin c-treated t4 cells (t4 mito) stimulated by immobilized 64.1 resulted in marked b cell proliferation and ig-secreting cells (isc) generation in the absence of any additional stimulation. the magnitude of the b cell responses induced by immobilized 64.1-stimulated t4 mito was far greater than that induced by other sti ...	1988	2967324
defective helper function of purified t4 cells and excessive suppressor activity of purified t8 cells in patients with b-cell chronic lymphocytic leukemia. t4 suppressor effector cells are present in certain patients.	we investigated helper and suppressor functions to b-cell responses and t-t cell interactions of purified t4 and t8 cells from 20 untreated patients with b-cell chronic lymphocytic leukemia (cll). appropriate mixtures of purified t4 or t8 cells from patients with cll were cultured with purified b cells or t4 and b cells from normal donors for 7 days with pokeweed mitogen (pwm). igm, iga, and igg produced were determined in the supernatants of these cultures by a heavy chain-specific enzyme-linke ...	1988	2967096
the clinical neuromuscular pharmacology of mivacurium chloride (bw b1090u). a short-acting nondepolarizing ester neuromuscular blocking drug.	mivacurium chloride (bw b1090u), a bis-benzylisoquinolinium diester compound, was found to undergo hydrolysis in vitro by purified human plasma cholinesterase in a ph-stat titrator at 88% of the rate of succinylcholine at ph 7.4, 37 degrees c and 5 microm substrate concentration. in 72 consenting asa physical status i-ii patients receiving nitrous oxide/oxygen-narcotic-thiopental anesthesia, the neuromuscular blocking effect of mivacurium was assessed following bolus doses from 0.03 to 0.30 mg/k ...	1988	2967039
hypothyroidism and pituitary contents of immunoactive met-enkephalin and beta-endorphin in male rats of different ages.	a comparison of the effect of ptu-induced hypothyroidism on the contents of immunoactive met-enkephalin and beta-endorphin in the pituitaries from rats aged 2 1/2, 12, and 18 months was made. in all ages, there was significant reduction of ir-met-enkephalin content in the anterior lobe and ir-beta-endorphin content in the neuro-intermediate lobe after ptu treatment. there was a significant age-related decrease in ir-beta-endorphin content in the anterior lobe. rats of all three ages responded to ...	1988	2966963
primary hypothyroidism in severe chronic heart failure.	thyroid function was studied in 40 patients with chronic heart failure. thyroid antibodies and microsome antibodies were negative in all cases. serum t4, and t3 concentrations showed significant inverse correlation with cardiothoracic ratio, mean right atrial pressure, pulmonary artery systolic pressure, and peripheral venous pressure. serum t4, t3 concentrations showed significant correlation with pao2, serum albumin, and serum cholinesterase. serum tsh concentrations increased with increasing ...	1988	2966870
t4+ t helper cell function in vivo: differential requirement for induction of antiviral cytotoxic t-cell and antibody responses.	this study documents the differential requirements of t4+ t helper cells in the induction of virus-specific cytotoxic t-lymphocyte (ctl) and antibody responses during acute lymphocytic choriomeningitis virus infection. two monoclonal antibodies (gk1.5 and rl172.4) directed against the l3t4 (t4) molecule were used for depleting t helper cells from mice. depletion of t4+ cells caused a pronounced suppression of antiviral antibody response (20-fold decrease) but had minimal effect on virus-specific ...	1988	2966865
human gamma interferon induction by staphylococcal protein a: effector cells, kinetics and the effect of prostaglandin, indomethacin, ibuprofen and aspirin.	soluble staphylococcal protein a (spa) induces the synthesis of gamma-interferon (gamma-ifn) by human peripheral blood lymphocytes (pbl). to investigate the kinetics of this gamma-ifn induction and the effector cells involved, we used a highly purified spa preparation, pbl from healthy volunteers, and a cpe-inhibition gamma-ifn assay with sindbis virus in human fibroblasts. the production of spa-induced gamma-ifn (spa-gamma-ifn) peaked 48 h after the addition of spa to cultures of pbl and decrea ...	1988	2966775
abnormalities in cd4+ t-lymphocyte subsets in inflammatory rheumatic diseases.	the monoclonal antibodies anti-2h4 and anti-4b4 identify the suppressor-inducer (cd4+2h4+) and helper-inducer (cd4+4b4+) subpopulations of cd4 (t4+) lymphocytes, respectively. the cell surface phenotype of peripheral blood lymphocytes and synovial fluid lymphocytes in patients with rheumatoid arthritis and other inflammatory joint diseases was analyzed by use of these and other well-characterized anti-t-cell monoclonal antibodies. in the synovial fluid of patients with rheumatoid arthritis, ther ...	1988	2966579
modulation of escherichia coli recbcd activity by the bacteriophage lambda gam and p22 abc functions.	plasmids that express the bacteriophage lambda gam gene or the p22 abc2 gene (with and without abc1) at controllable levels were placed in escherichia coli and tested for effects on the activity of recbcd. like gam, abc2 inhibited the atp-dependent exonuclease activity of recbcd, apparently not by binding to dna. however, abc2-mediated inhibition was partial, while gam-mediated inhibition was complete. both abc2 and gam inhibited host system-mediated homologous recombination in a chi-containing ...	1988	2966143
changes in the helper and suppressor lymphocytes in human peripheral blood following maximal physical exercise.	lymphocyte subpopulations and con a activated suppressors were measured before, immediately after maximal physical effort on bicycle ergometer and after two-hour recovery. the investigations were performed on healthy male volunteers, trained male and female cyclist, nearly at the same age. the measured parameters of maximal effort were comparable in all investigated groups. the significant increase in t8+ (suppressor cytotoxic) cells and moderate increase in t4+ (helper inducer) cells was observ ...	1987	2965563
cell-populations involved in the humoral leukocyte adherence inhibition reaction. the t8-suppressor/cytotoxic cells are both effectors and responders.	in an attempt to identify the cell subpopulation involved in the humoral leukocyte adherence inhibition (h-lai) reaction, mononuclear leukocytes were fractionated and the response with the different cell populations was measured in the h-lai assay using sera from lung cancer patients and a lung cancer associated antigen. the monocytes were found to exhibit a suppressive effect on the h-lai response. the b-lymphocytes seemed not to take an active part in the reaction. depletion of the t-lymphocyt ...	1987	2965016
studies of lymphocyte subpopulations and immunoglobulin production in iga nephropathy.	this work was undertaken to examine the immunoregulation of t lymphocytes in patients with iga nephropathy. fifty patients and thirty-seven control subjects were studied in an infection-free interval. t lymphocyte subpopulations were determined using okt monoclonal antibodies against helper (okt4) and suppressor (okt8) t cell subsets. the proportions of t lymphocyte subpopulations did not differ between patients and controls. patients with significant renal impairment demonstrated a reduced okt4 ...	1987	2964968
mechanism of t cell-derived helper factor production upon stimulation with pokeweed mitogen in humans.	t cell-derived helper factors can cause activated b cells to proliferate and differentiate into immunoglobulin (ig)-secreting cells. in the pokeweed mitogen (pwm)-driven ig production system, the cell-cell interaction necessary for the production of the helper factors was analysed. t cells were pulsed with pwm with or without autologous monocytes. the t cells were then isolated, and incubated for 48 h. supernatants were tested for their ability to promote differentiation of activated b cells. we ...	1988	2964963
treatment of the acquired immunodeficiency syndrome (aids) and aids-related complex with a regimen of 3'-azido-2',3'-dideoxythymidine (azidothymidine or zidovudine) and acyclovir. a pilot study.	on the basis of observation that acyclovir potentiates the in-vitro antiviral activity of 3-azido-2',3'-dideoxythymidine (also known as azidothymidine or zidovudine) against human immunodeficiency virus (hiv), we administered a regimen of azidothymidine and acyclovir to eight patients with the acquired immunodeficiency syndrome (aids) or aids-related complex. an oral regimen of 100 mg of azidothymidine and 800 mg of acyclovir every 4 hours was in general well tolerated, with the principal toxici ...	1988	2964805
nucleotide sequence of the tail sheath gene of bacteriophage t4 and amino acid sequence of its product.	the nucleotide sequence of gene 18 of bacteriophage t4 was determined by the maxam-gilbert method, partially aided by the dideoxy method. to confirm the deduced amino acid sequence of the tail sheath protein (gp18) that is encoded by gene 18, gp18 was extensively digested by trypsin or lysyl endopeptidase and subjected to reverse-phase high-performance liquid chromatography. approximately 40 peptides, which cover 88% of the primary structure, were fractionated, the amino acid compositions were d ...	1988	2964531
regulation of fructose 2,6-bisphosphate levels in cold-acclimated brown adipose tissue of rat.	the effects of cold exposure and t4 administration on fructose 2,6-bisphosphate levels, phosphofructokinase-2 and pyruvate kinase activities were examined in rat brown adipose tissue. cold adaptation (14 days) gave rise to a 2-fold increase in the amount of fructose 2,6-bisphosphate and phosphofructokinase-2 activity, and increased the pyruvate kinase activity 4-fold. if, in addition, the cold-acclimated rats were treated with t4, these parameters were again significantly enhanced. the effect on ...	1988	2964380
increased helper inducer and decreased suppressor inducer phenotypes in the rheumatoid joint.	cells isolated from the joints of patients with rheumatoid arthritis (ra) exhibit functional immune abnormalities, such as diminished suppressor activity, depressed response to mitogens, and enhanced immunoglobulin production. we sought to characterize the t lymphocyte subsets in the synovial fluid (sf) and peripheral blood (pb) of ra patients in an attempt to clarify the mechanism(s) responsible for these functional immune abnormalities. we used dual-immunofluorescence staining techniques with ...	1988	2964240
induction of helper and suppressor t cells by nonoverlapping determinants on the large protein antigen, beta-galactosidase.	the fine specificity of the t cell repertoire directed against t helper (th)-inducing and t suppressor (ts)-inducing determinants was examined with cyanogen bromide and tryptic peptides of escherichia coli beta-galactosidase (gz), a large tetrameric protein (monomer molecular weight = 116 kda). immunization with cyanogen bromide fragment 2 [cb-2, amino acids (a.a.) 3-92] induced both specific th and ts cells. study of the induction of these functionally opposite t cell subpopulations with trypti ...	1988	2963778
[t cell subpopulations in peripheral blood and liver tissue in acute hbsag-negative hepatitis].	by means of monoclonal antibodies against surface antigens of mononuclear cells we examined alterations of t-cell-subpopulations of peripheral blood and liver tissue in the course of acute hbsag-negative hepatitis. used methods for determination were the immunofluorescence for blood lymphocytes, respectively the peroxidase-anti-peroxidase-(pap)-technique for round-cell-infiltrates of the liver based on paraffin fixed sections. the focus of attention was the relation of t4-(helper/inducer) to t8- ...	1987	2963738
[effect of 3,3',5' triiodo-l-thyronine (rt3) on the serum concentration of thyroid hormones in rats].	50, 100 or 150 micrograms/100 g body weight/day of very pure 3,3',5' triiodo-l-thyronine (rt3), obtained from a new synthetic method, was intraperitoneally administered in male wistar rats for 5 weeks. serum total thyroxine (t4), free thyroxine (ft4) and total 3,5,3' triiodo-l-thyronine (t3) concentrations were increased with all the doses of rt3. free t3 (ft3) was also but non-significantly elevated. different assumptions are put forward in order to explain this rt3 effect.	1987	2963677
action of long-chain fatty acids in vitro on ca2+-stimulatable, mg2+-dependent atpase activity in human red cell membranes.	human red cell membrane ca2+-stimulatable, mg2+-dependent adenosine triphosphatase (ca2+-atpase) activity and its response to thyroid hormone have been studied following exposure of membranes in vitro to specific long-chain fatty acids. basal enzyme activity (no added thyroid hormone) was significantly decreased by additions of 10(-9)-10(-4) m-stearic (18:0) and oleic (18:1 cis-9) acids. methyl oleate and elaidic (18:1 trans-9), palmitic (16:0) and lauric (12:0) acids at 10(-6) and 10(-4) m were ...	1987	2963620
australian hemophiliac recipients of voluntary donor blood products longitudinally evaluated for aids. a clinical and laboratory study, 1983-1986.	one hundred and sixty-one australian patients with hereditary bleeding disorders comprising hemophilia a (120), hemophilia b (18), von willebrand's disease (16), and seven symptomatic female hemophilia a or b carriers were screened for clinical and serological evidence of exposure to htlv-iii/lav/arv/hiv infection. during the previous five years (1979-1984) they had been treated almost exclusively with blood products derived from australian voluntary donors. the prevalence of htlv-iii antibodies ...	1987	2963611
expression of t-cell antigens on reed-sternberg cells in a subset of patients with nodular sclerosing and mixed cellularity hodgkin's disease.	expression of t-cell antigens by reed-sternberg (rs) cells has not been detected in most studies of hodgkin's disease (hd). the authors employed an improved method of fixation (paraformaldehyde-lysine-periodate), which sharply defined cell borders and revealed t-cell antigens on rs cells in 8 of 30 (27%) cases of hd. antigen-specific staining was confirmed by immunoelectron microscopy. rs cells expressed t11 (8/8 cases), leu-3 or t4 (4/8 cases), leu-4 or t3 (3/8 cases), but not other t-cell spec ...	1988	2963547
nucleotide sequence of the tail tube structural gene of bacteriophage t4.	the nucleotide sequence of gene 19 of bacteriophage t4, the structural gene of the tail tube protein, was determined by both the dideoxy and the maxam-gilbert methods. the predicted mr of tube protein gene product 19 is 18,842. the n-terminal amino acid of the tube protein was determined by edman degradation, and the c-terminal sequence was confirmed by isolation of the c-terminal tryptic peptide. in the noncoding region between genes 18 and 19, there are two late-t4-promoter consensus sequences ...	1988	2963141
multiple sclerosis: ii. effects of prothymosin alpha on the autologous and allogeneic mlr in patients with multiple sclerosis.	we have recently demonstrated that peripheral blood monocytes from patients with multiple sclerosis (ms) have a defect in stimulating autologous and allogeneic t lymphocytes. this defect was found to correlate with disease activity. in this report we demonstrate that prothymosin alpha (prot alpha), a rat thymus fraction 5 polypeptide, restores the ms monocyte stimulatory defect. the concentrations of prot alpha which induced optimal enhancement of the mixed lymphocyte responses (mlr) were signif ...	1987	2962790
in vitro anti-hbs antibody synthesis from anti-hepatitis b vaccine recipients.	peripheral blood mononuclear cells (pbmc) from 'responders' recently boosted with hepatitis b vaccine, were studied for synthesis in vitro of antibody to hepatitis b surface antigen (anti-hbs ab) when stimulated with pokeweed mitogen (pwm) or hbsag. hbsag alone can induce an antigen-specific anti-hbs ab response in vitro; this antibody synthesis is t cell-dependent. in some responders both allogeneic t4+ cells (in absence of pwm or hbsag) and mixed leucocyte culture supernatants (mlc/sn) (withou ...	1987	2962789
cd11 molecule defines two types of suppressor cells within the t8+ population.	it has been shown that t8+ cells are comprised of functionally heterogeneous subpopulations such as suppressor, cytotoxic, and nk cells. in this report, we attempted to delineate the functional heterogeneity of t8 cells defined by anti-cd11 antibody (anti-mol). although allospecific cytotoxic activity was restricted to the t8+mol- subset, suppression of pwm igg synthesis could be elicited in both the t8+mol+ and the t8+mol- subset of cells. however, the mechanism of suppression was different in ...	1988	2962748
generation of cd8 (t8) cytotoxic cells has a preferential requirement for cd4+2h4- inducer cells.	cd8 (t8) cells are capable of both suppression and cytotoxicity. however, we have found that the activation of cd8 cytotoxic cells has a preferential requirement for a different cd4 (t4) subset from that previously reported for the activation of cd8 suppressor cells. we have recently characterized two monoclonal antibodies which subdivide cd4 cells into inducers of help for antibody production (cd4+ 4b4+) and inducers of cd8 mediated suppression (cd4+2h4+). we now report that cd4+4b4+2h4- cells ...	1988	2962747
familial splenomegaly syndrome with reduced circulating t helper cells and splenic germinal centre hypoplasia.	familial splenomegaly is a rare occurrence and is occasionally associated with immune abnormalities. we report three members of a family with massive splenomegaly associated with a reduction in circulating t helper cells, a reversed t4/t8 ratio and cutaneous anergy. the spleen and lymph nodes were shown in one family member to have germinal centre hypoplasia with t helper cells being present in normal numbers and distribution in these tissues. various abnormalities of immunoglobulins were also n ...	1987	2962629
activation of resting human b cells by helper t-cell clone supernatant: characterization of a human b-cell-activating factor.	the effects of helper t-cell clone supernatants on resting human b cells were investigated. four different helper t-cell clones (two t4+ and two t8+) were stimulated by anti-t3 monoclonal antibodies on sepharose beads or anti-t11(2) plus anti-t11(3) monoclonal antibodies. the supernatants from these activated clones induced the proliferation of highly purified resting b lymphocytes from the peripheral blood. the b cells exhibited a cell size and a surface-antigen pattern (4f2 antigen and transfe ...	1987	2962196
[role of ultrasensitive tsh assay and ultrarapid total thyroxine assay in the thyroid studies].	the diagnostic value of high sensitivity thyrotrophin assay (hs-tsh) and that of ultra-short determination of total thyroxine (t4) by fluorescence polarization (t4-tdx) were evaluated in 284 patients (29 hyperthyroid, 137 euthyroid without treatment and 118 under substitutive therapy). after comparing the clinician's first impression with the results of these laboratory tests the proportion of correctly classified untreated euthyroid patients was the same with t4-tdx as with hs-tsh (90%). in con ...	1987	2962120
neonatal t4+ lymphocytes: analysis of the expression of 4b4 and 2h4 antigens.	using monoclonal antibodies anti-2h4 and anti-4b4 we investigated by double immunofluorescence and immunorosette technique the presence of cells displaying the helper-inducer and the inducer of suppression phenotype among t4+ cord blood cells. the analysis of the estimated frequencies of subpopulations of t4+ cells shows that a high percentage of t4+ cord blood cells can coexpress both antigens in comparison to adult. furthermore the percentage of t4+ cells with helper-inducer phenotype (2h4-4b4 ...	1988	2961493
t4-induced cardiac hypertrophy and the perfused and total microvasculature of the heart.	the purpose of the present investigation was to determine the effects of thyroxine (t4), which induces myocardial hypertrophy, on the number per square millimetre and volume per cubic millimetre of both the total and perfused portions of the arteriolar and capillary beds of the heart. studies were conducted in the subendocardial and subepicardial regions of the left ventricle of anesthetized open-chest rabbits. fluorescein isothiocyanate-dextran (i.v.) or radioactive microspheres (intra-atrial) ...	1987	2961428
defects of autologous mixed lymphocyte reaction-activated immunoregulatory t cells in patients with systemic lupus erythematosus.	the present study was undertaken to determine the nature of the immunoregulatory t-cell defect after autologous mixed lymphocyte reaction (amlr) activation in patients with systemic lupus erythematosus (sle). although amlr was decreased in patients with sle compared with normals, there was no difference in major proliferative cells (t4 cells and t4+jra+ subset) in response to amlr. functional activity of amlr-stimulated t4 subsets in patients with sle and normals was examined in helper and suppr ...	1987	2961048
[structure and regulation of the assembly of bacteriophage t4 fibrillar proteins. ii. isolation and initial structural characteristics of whiskers].	a procedure for purification of bacteriophage t4 whiskers and it's monomeric subunits--gene product wac--has been developed. we have shown, that the whiskers are composed of two identical copies of gene product wac with molecular weight of 56 kda each. the dimer of gene product wac is a highly ordered structure and it's length is about 70.0 +/- 10.0 nm, as revealed by electron microscopy. the amino acid composition of whiskers is very similar to that of watersoluble keratins. we have proposed a ...	1987	2960883
[structure and regulation of the assembly of bacteriophage t4 fibrillar proteins. i. isolation and spectral properties of long fibers].	a preparative procedure for purification of the biological active proximal (a) and distal (bc') parts of bacteriophage t4 long-tail fibers is described. absorption spectra of these proteins in the near ultraviolet region were measured. the absorption coefficients were determined on the basis of the nitrogen content, the absorption coefficient for the a part is epsilon 0.1% 277 nm = 0.93 +/- 0.06 and for the bc' part is epsilon 0.1%, 277,5 nm = 1.01 +/- 0.08. calculations of the secondary structu ...	1987	2960882
wild-type bacteriophage t4 is restricted by the lambda rex genes.	the bacteriophage t4 rii genes and the lambda rex (r exclusion) genes interact; rii mutants are unable to productively infect rex+ lambda lysogens. the relationship between rex and rii has been found to be quantitative, and plasmid clones of rex have excluded not only rii mutants but t4 wild type and most other bacteriophages as well. mutations in the t4 mota gene substantially reversed exclusion of t4 by rex.	1987	2960831
prohead and dna-gp3-dependent atpase activity of the dna packaging protein gp16 of bacteriophage phi 29.	the atpase activity of the dna packaging protein gp16 (gene product 16) of bacteriophage phi 29 was studied in the completely defined in-vitro assembly system. atp was hydrolyzed to adp and pi in the packaging reaction that included purified proheads, dna-gp3 and gp16. approximately one molecule of atp was used in the packaging of 2 base-pairs of phi 29 dna, or 9 x 10(3) atp molecules per virion. the hydrolysis of atp by gp16 was both prohead and dna-gp3 dependent. gp16 contained both the "a-typ ...	1987	2960820
the subdivision of the t4 (cd4) subset on the basis of the differential expression of l-c/t200 antigens.	the t4 (cd4) subset of t lymphocytes has been subdivided into two major subsets, a suppressor/inducer subset (t4+,2h4+) and a helper subset (t4+,2h4-) on the basis of the differential expression of the l-c/t200 (cd45) antigens. the 2h4 antigen itself comprises at least three distinct polypeptides at 125,200, and 220 x 10(3) mr, of which the 200 and 220 x 10(3) mr polypeptides constitute the highest mr isoforms of a pool of five distinct l-c/t200 antigens. the t4+,2h4+ subset expresses at least f ...	1987	2960772
an antigen-specific t8+ human clone of cells with a nonspecific augmenting function on the t4 cell-b cell helper interaction.	we isolated a t8+ t3+ ia+ clone of cells from the peripheral blood mononuclear cells of a healthy subject. the clone was expanded and maintained with autologous feeder cells, interleukin 2, and a streptococcal antigen. the t8+ clone of cells responded specifically to the streptococcal antigen, in the absence of accessory cells, and released a soluble factor. both the cloned cells and the corresponding soluble factor expressed augmenting helper but not suppressor activity. the augmenting helper a ...	1987	2960732
failure of t3 to potentiate tricyclic antidepressant response.	despite a lack of documented efficacy in controlled trials, triiodothyronine (t3) is frequently administered as an adjunctive therapy for tricyclic resistant depressions. in this study, we tested the efficacy of t3 as an adjunctive treatment using a double-blind, placebo-controlled crossover design. sixteen depressed patients who were unresponsive to 4 weeks of imipramine therapy (mean dose = 206 +/- 54 mg daily, mean combined blood level = 220 +/- 132 ng/dl) received t3 25 micrograms and placeb ...	1987	2960719
plasma atriopeptin concentrations in hyperthyroidism, euthyroidism, and hypothyroidism: studies in man and rat.	atriopeptin (ap) is a polypeptide produced by atrial myocytes that is capable of inducing diuresis, natriuresis, and vasodilatation. because thyroid dysfunction is known to be associated with alterations in both renal function and vasomotor control, we investigate the possible effects of varying thyroid function on ap in humans and rats. plasma ap concentrations were determined in hyperthyroid and hypothyroid patients and normal subjects. plasma ap was also measured in some patients after the iv ...	1987	2960689
an open reading frame in the escherichia coli bacteriophage lambda genome encodes a protein that functions in assembly of the long tail fibers of bacteriophage t4.	assembly of the long tail fibers of the escherichia coli bacteriophage t4 requires the catalytic action of two auxiliary proteins. it was found that a gene of the entirely unrelated phage lambda codes for a protein which can substitute for one of these t4 polypeptides, protein 38. the lambda gene was designated tfa (tail fiber assembly). protein 38 consists of 183 residues, and the tfa protein consists of 194 residues; the two polypeptides are about 40% homologous. although the tfa gene is dispe ...	1987	2960666
human t4+ t-lymphocyte clones specific for the b fragment of tetanus toxin.	two t4+ cloned t-lymphocyte lines specific for a papain digest product of tetanus toxin are functionally characterized. the two clones were obtained from peripheral blood mononuclear cells activated in vitro by tetanus toxoid, expanded with il-2, and cloned in soft agar. both clones could be induced to undergo blastogenesis with tetanus toxoid, tetanus toxin, and the b fragment but not the c fragment of tetanus toxin. in addition, both clones caused cytolysis of plastic adherent cell targets coc ...	1987	2960640
inhibition of streptomycin-dependent mutation in escherichia coli on the lytic growth of bacteriophage lambda.	spontaneous streptomycin-dependent mutants (strda) were isolated from escherichia coli c600. on c600 strd, the lytic growth of phage lambda nam and lambda ci857 was inhibited. after e. coli lysogenic strain 1.1485 (lambda ci857) mutated to strda, induction of lambda was decreased greatly. on strda of e. coli strains c600 and 1.1485 (lambda ci857), the plating efficiencies and burst sizes of phage t4 and t7 remained normal. since strda is a mutation in the structural gene for ribosomal protein s1 ...	1987	2960017
molecular approaches toward a therapy for multiple sclerosis.	treatment of experimental allergic encephalomyelitis with monoclonal antibodies to products of immune response genes and to surface markers on t cells was reviewed. both anti ia therapy and anti t4 therapy can reverse ongoing paralysis, and block subsequent relapses. the appearance of t4 cells and ia antigens in relapse was demonstrated. the prospects for human therapy with chimeric human-mouse immunoglobulins and isotypic variants was discussed, with particular attention to the immunotherapy of ...	1987	2960007
activities involved in base excision repair of bacteriophage t4 and lambda dna in vivo.	the in vivo excision repair functions of escherichia coli exonuclease iii and 3-methyladenine dna glycosylase i, and bacteriophage t4 pyrimidine dimer-dna glycosylase were investigated. following exposure of bacteriophage t4 or lambda to methyl methanesulfonate or ultraviolet irradiation, survival was determined by plating on e. coli have various genetic backgrounds. although exonuclease iii was shown to participate in base excision repair initiated by 3-methyladenine dna glycosylase i, it had n ...	1987	2959841
immunoregulatory t cells in alcoholic liver disease: phenotypical dissection of circulating leu3+/t4+ inducer t-lymphocytes.	alcoholic liver disease (ald) patients had normal absolute lymphocyte counts, increased percentage of leu3+/t4+ cells (p less than 0.001) and raised t4/t8 ratio (p less than 0.01). double-colour immunofluorescence analysis using isotype-specific goat anti-mouse immunoglobulins, fluorescein or rhodamine-conjugated, demonstrated that the rise in inducer (leu3+/t4+) t-cells was almost entirely represented by an expanded population of t4+tq1- and 5/9+ true helper lymphocytes. t4+ cells also expresse ...	1987	2959783
abnormal lymphocyte reactivity to self-major histocompatibility antigens in rheumatoid arthritis.	proliferation of rheumatoid and control peripheral blood mononuclear cells to okt3, phorbol myristic acid (pma), phytohemagglutinin (pha), tuberculin ppd and in the autologous mixed lymphocyte reaction (amlr) was investigated. only the responses to ppd and in the amlr were depressed. this was not due to suppression by okt8 lymphocytes. the proportion of antigen responsive (t4+ 4b4+) cells was normal but suppressor-inducer (t4+ 2h4+) cells were decreased. the depressed response was not completely ...	1987	2959772
altered t4/t8 ratios in a patient with severe recurrent aphthous ulcers: report of a case.		1987	2959760
immunoregulatory effects of intravenous immune serum globulin therapy in common variable hypogammaglobulinemia.	because of evidence of immunoregulatory effects of short-term administration of pooled donor gamma globulins, the effects of intravenous immune serum globulin were studied in nine adult patients with common variable hypogammaglobulinemia over a two-year period. baseline assessment prior to immune serum globulin replacement included evaluation of b cell function, suppressor cell activity, and t cell subsets. these analyses were subsequently performed during the course of a first-year treatment pe ...	1987	2959150
receptor-recognizing proteins of t-even type bacteriophages. constant and hypervariable regions and an unusual case of evolution.	proteins 38 of bacteriophages t2, k3, ox2 and m1 are located at the free ends of their long tail fibers and function as adhesins, i.e. they mediate binding to the bacterial receptors. the latter three phages use the escherichia coli outer membrane protein ompa as a receptor, while t2 uses the outer membrane proteins ompf or ttr. the dna sequences of genes 38 of phages ox2 and m1 have been determined and are compared with those known for t2 and k3. the genes encode 262(t2), 260(k3), 266(ox2) and ...	1987	2958637
antigen-induced human interferon-gamma production. differential dependence on interleukin 2 and its receptor.	once stimulated with toxoplasma gondii or cytomegalovirus (cmv) antigens, peripheral blood mononuclear cells from healthy seropositive donors secrete comparable levels of interferon-gamma (ifn-gamma). both antigens also stimulated specific production of interleukin 2 (il-2), a lymphokine believed to be important in ifn-gamma generation. t. gondii antigen, however, induced ninefold more il-2 than did cmv antigen suggesting different mechanisms for antigen-stimulated ifn-gamma production. therefor ...	1987	2958545
cd4 (t4+) lymphocytes in semen of healthy heterosexual men: implications for the transmission of aids.		1987	2958370
selective igm deficiency in adults: phenotypically and functionally altered profiles of peripheral blood lymphocytes.	peripheral blood lymphocytes from four patients with selective igm deficiency were examined phenotypically and functionally. although b cell subpopulations determined by surface immunoglobulins were within normal or nearly normal range, t8+ cells were significantly increased and t4/t8 ratios were inverted in three patients. igm specific hyporesponsiveness in the pwm-driven immunoglobulin production system was observed in all four patients. ia-like antigen positive t cells were increased in two p ...	1987	2958191
subpopulations of cd4+ (t4+) cells in homosexual/bisexual men with persistent generalized lymphadenopathy.	using monoclonal antibodies (anti-2h4 and anti-4b4), cd4+ (t4+) t cells are further subdivided into two major subpopulations of inducers of help (t4+4b4+) and inducers of suppression (t4+2h4+). to determine whether human immunodeficiency virus (hiv) infection results in the depletion of a subset of cd4+ cells, peripheral blood mononuclear cells from patients with persistent generalized lymphadenopathy (pgl) were quantified for the subpopulation of cd4+ t cells, using anti-2h4 and anti-4b4 monocl ...	1987	2958183
preoperative renal tumor embolization. a useful procedure?	the medical reports of 113 patients operated upon for renal tumors were retrospectively reviewed to assess the value of preoperative embolization. coils or ethanol were used preoperatively in 55 patients while 58 patients underwent nephrectomy without embolization. the tumors were staged according to the tnm and the robson staging classification. t1 and t2 tumors as well as t3 and t4 tumors were grouped together for statistical evaluation. there was no significant difference in intraoperative bl ...	1987	2958037
lymphocyte subsets in urologic cancer patients.	peripheral blood t lymphocyte subsets were quantitated by monoclonal antibodies in 91 patients with urologic malignancies as well as eight age-matched controls. in general, the presence of cancer and increasing stage were associated with a relative decrease in helper and an increase in suppressor t-cell activity as well as a decreased t4/t8 cell ratio. therapy in the form of androgen depletion reversed these trends and restored helper and suppressor levels as well as the t4/t8 ratio to control v ...	1987	2957843
phenotypes of t lymphocytes from peripheral blood and synovial fluid of patients with rheumatoid arthritis and juvenile rheumatoid arthritis. evidence in favour of normal helper and suppressor functions of t lymphocytes from patients with juvenile rheumatoid arthritis.	lymphocytes from peripheral blood (pb) and synovial fluid (sf) from 21 patients with rheumatoid arthritis (ra) and 18 patients with juvenile rheumatoid arthritis (jra) were studied with respect to t cell phenotypes using monoclonal antibodies in a rosette assay. the percentage of hla-dr positive t cells was counted in pb and sf using indirect immunofluorescence. suppressor cell activity of t cells from pb and sf was investigated by measuring the immunoglobulin production by pokeweed mitogen (pwm ...	1987	2957788
a major mechanism of human immunodeficiency virus-induced cell killing does not involve cell fusion.	in vitro studies indicate that human immunodeficiency virus (hiv) infections are cytopathic for t4+ peripheral blood lymphocytes and for most continuous lines of t4+ lymphocytes. these cytopathic effects have been largely attributed to the formation of syncytia by hiv-infected cells. we report that hiv infections killed cultured peripheral blood lymphocytes and a line of t4+-lymphoid cells (cem cells) without causing cell fusion. we also report that the occurrence of syncytia is an early and tra ...	1987	2957508
phenotypic and functional analysis of t cells extracted from chronically inflamed human periodontal tissues.	t-cell subsets extracted from chronically inflamed periodontal tissues were identified using monoclonal antibodies, and their functional activity was analysed using the autologous mixed lymphocyte reaction (amlr). tissue was obtained from a total of 33 adult periodontitis (ap) patients and 6 normal/marginal gingivitis (n/mg) patients. all ap patients had received repeated oral hygiene instruction and root planing prior to the surgery, and the majority (30 out of 33) had at least one site with gr ...	1987	2957490
complexity of t cell receptor recognition sites for defined alloantigens.	three monoclonal antibodies react with the t cell receptor on the tumor line hpb-all and in addition with 3 to 13% of human peripheral blood t cells of normal donors. these antibodies are shown to react with an epitope encoded by the v beta 5 family of t cell receptor beta-chain variable region gene segments. cells expressing v beta 5 gene segments can have cytotoxic or helper function, be of the t4+ or t8+ phenotype, and have specificity for either class i or class ii major histocompatibility c ...	1987	2957443
lymphocyte phenotypes at tumor margins in patients with head and neck cancer.	lymphocyte phenotypes were identified by monoclonal antibodies and avidin-biotin peroxidase reagents and enumerated in tumor tissue removed from 26 patients with head and neck cancer. t lymphocytes (t11) were the predominate phenotype at the tumor margin, with twice as many helper/inducer (t4) as suppressor/cytotoxic (t8) lymphocytes. patients with stage ii and iii carcinoma of the glottis, tongue, and hypopharynx had significantly increased numbers of all t lymphocyte phenotypes when compared t ...	1987	2957343
regulation by thyroid hormones of terminal differentiation in the skeletal dorsal muscle. ii. urodelan amphibians.	in the urodelan amphibian pleurodeles waltlii, spontaneous anatomical metamorphosis was correlated with an increase in the serum level of thyroxine (t4). it was also accompanied by a change in the myofibrillar atpase profile of the dorsal skeletal muscle; fibers of larval type were gradually replaced by the adult fiber types i, ii a, and ii b. likewise, a myosin isoenzymic transition was observed in dorsal muscle, larval isomyosins were replaced by adult isoforms. in a related species, ambystoma ...	1987	2957262
responses of peripheral blood cells and lymphocyte subpopulations to insulin-induced hypoglycaemia in human insulin-dependent (type 1) diabetes.	changes in counts of peripheral blood cells including lymphocytes and lymphocyte subpopulations were examined in response to acute insulin-induced hypoglycaemia in fourteen insulin-dependent diabetic patients of differing duration, and in eight normal subjects. in patients with a short duration of diabetes (less than 5 years) an initial lymphocytosis preceded a later granulocytosis, similar to changes in the normal group following hypoglycaemia. the lymphocytosis comprised increments in t11 (tot ...	1987	2957208
lymphocyte subpopulation profiles and mitogen kinetics in immunological deficiency testing.	the correlation between lymphocyte proliferative responses to mitogens and t4/t8 ratios was analyzed in a cross section of patients who either were in a high-risk group for htlv-iii infection or fulfilled clinical criteria for acquired immune deficiency syndrome (aids). the patient results showed that, correlated with decreased t4/t8 ratios, there was a decrease in mitogen responsiveness first to pokeweed mitogen (pwm), followed by concanavalin a (con a) and then phytohemagglutinin (pha). parall ...	1987	2956275
organization of double-stranded dna in bacteriophages: a study by cryo-electron microscopy of vitrified samples.	in this paper it is shown that conformation and packing of double-stranded dna within the head of bacteriophages lambda and t4 can be assessed by cryo-electron microscopy of vitrified specimens. electron diffraction patterns show that dna within vitrified bacteriophages has a b conformation. electron micrographs of vitrified bacteriophages show domains within the head formed by a approximately 2.5-nm striation and arising from the dna packing. the number of differently oriented domains seen with ...	1987	2956092
activities of glycolytic enzymes in some brain areas of thyroidectomized rats and their response to replacement therapy.	glycolytic metabolism has been assessed by studying a set of key enzymes, in anterior cortex, amygdala, hypothalamus septum and hippocampus, in thyroidectomized rats. the reversibility of the changes induced by the thyroidectomy has been assessed by replacement therapy. in thyroidectomized rats the hexokinase activity was significantly decreased in anterior cortex and hypothalamus. the increase in phosphofructokinase and pyruvate kinase activity was probably due to an increase in cellular energy ...	1987	2956088
studies on human blood lymphocytes with ic3b (type 3) complement receptors: iii. abnormalities in patients with active systemic lupus erythematosus.	lymphocytes displaying ic3b (type 3) complement receptors (cr3) were quantified by flow cytometry in patients with systemic lupus erythematosus. the percentages and absolute numbers were compared to age and sex matched controls. total cr3+ lymphocytes identified by the monoclonal antibodies okm1 or leu 15 were significantly decreased in patients with symptomatic arthritis, serositis or vasculitis and those with lupus nephritis, whereas values for cr3+ lymphocytes in patients with inactive diseas ...	1987	2955974
antigen presentation by a subset of t8+ ia+ cells to t4+ helper cells.	the helper function of human t4+ cells acting on autologous peripheral blood b cells was elicited by the hapten-carrier conjugate dnp-streptococcal antigen (dnp-sa), in the presence of monocyte-enriched cells. the antigen presenting function of monocyte-enriched cells can be replaced by a subset of autologous t8+ vicia villosa lectin adherent (t8vv+) cells. cell depletion studies confirmed that the t8vv+ cell presentation was mediated by a t cell since killing with anti-t3 antibody and complemen ...	1987	2955972
lymphocyte transformation induced by autologous cells: xviii. impaired autologous mixed lymphocyte reaction in subjects with aids-related complex.	t cells from patients with aids-related-complex (arc) and normal controls were studied for their proliferative response in the autologous mixed lymphocyte reaction (amlr). cells from arc subjects failed to proliferate in autologous mlr compared to normal controls. this defect was not attributable to an altered ratio of t4 to t8 cells, as purified t4 cells from arc subjects also failed to proliferate in autologous mixed lymphocyte culture. in contrast, t cells from arc subjects proliferated norma ...	1987	2955966
circatrigintan hormonal and skin surface temperature rhythms following hysterectomy.	starting 2.5 weeks after removal of her uterus, but not of her ovaries, a 34-year-old, clinically healthy woman contributed a daily blood sample at 0900 and measured her skin surface temperature on her right breast above the nipple and just below the right breast daily for the ensuing 2 months. in aliquots of serum stored frozen, luteinizing hormone (lh) and follicle-stimulating hormone (fsh) were determined in the united states; these hormones and prolactin, estradiol (e2), progesterone, testos ...	1987	2955424
the t4 molecule differentially regulating the activation of subpopulations of t4+ cells.	it has been demonstrated that the t4+2h4+ subset functioned as a suppressor inducer cell, whereas the reciprocal t4+2h4- subset provided help for b cell ig production. in the present studies, a series of monoclonal antibodies to cell surface structures expressed on these subsets of cells were examined for their effects on the proliferative and immunoregulatory functions generated in amlr. we demonstrated that anti-t4 antibody preferentially inhibited the proliferative response of the t4+2h4+ but ...	1987	2955047
immunoregulatory effects of fraction 5 thymus peptides. i. thymosin alpha 1 enhances while thymosin beta 4 suppresses the human autologous and allogeneic mixed lymphocyte reaction.	thymosin alpha 1 and thymosin beta 4, two peptides isolated from preparations of calf thymus fraction 5, were tested in the human mixed lymphocyte reaction (mlr). thymosin alpha 1 was found capable of enhancing both the allogeneic and autologous mlr. on the contrary, thymosin beta 4 suppressed mlr proliferative responses. study of the responses of the t cell subpopulations revealed that t4+ (helper/inducer) cells but not t8+ (suppressor/cytotoxic) are responsible for the enhanced, proliferative ...	1987	2954929
stimulation and inhibition of human granulopoiesis in vitro by normal and malignant t4- or t8-lymphocyte subpopulations.	we studied the role of total peripheral blood t-lymphocytes and separated subpopulations of okt4+ and okt8+ cells stimulated with concanavalin a in the regulation of human granulopoiesis. unfractionated total t cells depleted of monocytes are capable of producing colony-stimulating activity (csa) and colony-forming unit-clone (cfu-c) suppressor activity simultaneously. after positive selection of cell subsets using a fluorescence-activated cell sorter, these two activities were produced by okt4+ ...	1987	2954841
expression of fc epsilon receptors on activated human t lymphocytes.	our results clearly demonstrate that the low-affinity receptor for ige (fc epsilon r) is an activation antigen transiently expressed on a subpopulation of human t lymphocytes. it can be selectively induced by stimulation with certain antigens or lectins, but it is not found on resting t cells. the increased numbers of activated fc epsilon r+ t cells observed after stimulation of peripheral blood mononuclear cells (pbmc) from bee venom allergic patients with the specific allergen phospholipase a2 ...	1987	2954825
abnormal lymphocyte function in scleroderma: a study on identical twins.	this paper describes immunologic studies on a set of identical twins discordant for the presence of scleroderma. the affected twin had a low absolute t-cell count, low numbers of t4 helper/inducer cells, and an increase in the t8 suppressor/cytotoxic cell count. the t cells of the patient responded poorly to mitogens and to allogeneic and autologous stimuli. by contrast, t-cell-helper activity for pokeweed mitogen-induced igm synthesis was markedly enhanced in the patient. furthermore, activated ...	1987	2954728
effect of amiodarone on rat heart myosin isoenzymes.	the effects of amiodarone on heart weight, production of 14c-co2 from labelled glucose, myosin atpase activity, and myosin isoenzyme patterns were determined by comparing control and amiodarone-treated male wistar rats. since it has been suggested that amiodarone may interfere with thyroid hormone action on the heart, similar experiments were also carried out in hypothyroid and amiodarone-plus-triiodothyronine(t3)-treated rats, and the data were compared to those obtained in amiodarone-treated r ...	1987	2954716
[pan t, t4 and t8-positive lymphocytes in the peripheral blood of patients with systemic lupus erythematosus].	in a group of 23 patients with systemic lupus erythematosus by means of monoclonal antibodies differentiation markers were investigated on peripheral blood t-lymphocytes. individual specific differences in the percentage of t1-, t3-, t4- and t8-positive cells were found, indicating various possibilities of immunological disorders in this disease. furthermore, in comparison with healthy donors, an increased number of t4-, t8-double stained cells was detected in the peripheral blood of patients wi ...	1987	2954308
lymphocyte subpopulations infiltrating squamous carcinomas of the head and neck: correlations with extent of tumor and prognosis.	because little is known about the mechanisms involved in local tumor-host immune reactions in squamous carcinoma of the head and neck, a study was undertaken to better characterize the types of immune cells present at the local tumor site and determine their relationship to tumor extent, systemic cellular immune parameters, and clinical outcome. in 40 untreated patients, lymphocyte subsets (ls) at the tumor-host interface were quantitated immunohistologically from serial sections of frozen tumor ...	1986	2954014
effects of escherichia coli ssb protein on the single-stranded dna-dependent atpase activity of escherichia coli reca protein. evidence that ssb protein facilitates the binding of reca protein to regions of secondary structure within single-stranded dna.	the effect that escherichia coli single-stranded dna binding (ssb) protein has on the single-stranded dna-dependent atpase activity of reca protein is shown to depend upon a number of variables such as order of addition, magnesium concentration, temperature and the type of single-stranded dna substrate used. when ssb protein is added to the dna solution prior to the addition of reca protein, a significant inhibition of atpase activity is observed. also, when ssb protein is added after the format ...	1987	2953903
identification and cloning of a prethymic precursor t lymphocyte from a population of common acute lymphoblastic leukemia antigen (calla)-positive fetal bone marrow cells.	we have cloned common acute lymphoblastic leukemia (calla)-positive cells from human fetal bone marrow containing less than 1 in 10,000 e-rfc in round-bottomed microtiter wells (one cell per well) using the autocloning unit of an epics-v cell sorter. expansion of such cells (with il-2 and heavily irradiated autologous thymocytes as feeder cells) resulted in growth in 6-14% of the wells (mean, 11%) with cells with mature t lymphocyte phenotype. two-color fluorescence analysis of outgrowing cultur ...	1987	2953847
[transmission of amber mutants of bacteriophage t4. iii. thermostability of the replication of amber mutants in cells of a non-permissive host is typical for the majority of phage tail genes].	the article deals with determination of the spreading of the earlier discovered phenomenon of the temperature sensitivity of multiplication of t4 phage amber mutants. on the basis of the study of the dependence of multiplication of 50 amber mutants in 22 genes of t4 phage tail in the cells of non-permissive host on the incubation temperature in the range of 15-41 degrees c, the following conclusion is drawn: temperature sensitivity of multiplication of amber mutants appears to be gene-specific a ...	1987	2953651
functional studies of t4+ lymphocyte subsets distinguished by the monoclonal antibody 5/9: suppressor-effector t4+ lymphocytes derive from the 5/9+ subset.	human t lymphocytes bearing the cell surface antigen t4 are functionally heterogeneous, exerting helper/inducer, suppressor-inducer, suppressor-effector, and cytotoxic activities. other cell surface antigens with a more restricted expression may help separate t4+ lymphocytes into functionally distinct subsets. this report describes the regulatory functions of t4+ lymphocytes fractionated by the monoclonal antibody 5/9, which detects a cell surface antigen present on 50-60% of t4+ lymphocytes. th ...	1987	2953445
the 2h4 molecule but not the t3-receptor complex is involved in suppressor inducer signals in the amlr system.	it is suggested that autologous mixed lymphocyte reaction (amlr) may play an important role in generating suppressor inducer signals and in down-regulating the immune response following self-major histocompatibility recognition. in the present study, monoclonal antibodies directed at cell surface structures on t4+ cells activated in amlr were used to define the molecules important in the generation of the suppressor inducer signal. the density of a 200/220-kda structure, termed 2h4, increased on ...	1987	2953440
expression of c-myc in stimulated t lymphocytes of the helper/inducer phenotype producing lymphokine(s) supporting multilineage colony formation.	phytohemagglutinin (pha) renders human peripheral t lymphocytes competent to replicate their dna and divide. the stimulation of peripheral t cells of the t4 phenotype by pha, which appears to be a transcription-dependent event, leads to the production and release of lymphokines supporting proliferation and differentiation of human pluripotent stem cells (cfu-gemmt). supernatants of pha-stimulated lymphocytes of the suppressor/cytotoxic phenotype (t8) failed to demonstrate reasonable activity to ...	1986	2953163
neurosarcoidosis: cerebrospinal fluid lymphocyte subpopulations.	csf lymphocyte subpopulations of eight patients with neurosarcoidosis were examined. csf or ct was abnormal in all. the csf t4/t8 (helper/suppressor) ratio was elevated at 6.8 and 7.6 in two patients; in one, there were only csf t4 cells. the ratio was normal in five patients.	1987	2952900
lymphocyte subpopulations in rheumatic heart disease.	monoclonal antibodies and indirect immunofluorescence techniques were used to compare the distribution of lymphocyte subpopulations of tonsil and peripheral blood from patients with rheumatic heart disease and age and socioeconomically matched patients undergoing tonsillectomy for chronic recurrent tonsillitis, but who had no evidence of rheumatic fever or rheumatic heart disease. the proportions of b cells (ba-1+), total t cells (lyt-3), inducer/helper t cells (t4+) and cytotoxic/suppressor t c ...	1987	2952796
predicted structure of tail-fiber proteins of t-even type phages.	the sequences of the tail fiber protein 36 of the phages t4, t2, k3, and ox2 were analyzed for homologies and for folding patterns using structure prediction methods. no repeating motif was found. a model for the fiber structure is proposed in which beta-strands of about 6 amino acids are separated by turns. in the beta-strand, hydrophobic amino acids are found alternating with hydrophilic ones. such amphipathic beta-strands can be stabilized by dimer formation. the dimerization occurs in a para ...	1987	2952522
different growth rates of swarm chondrosarcoma in lewis and wistar rats correlate with different thyroid hormone levels.	the transplantable swarm rat chondrosarcoma grew to twice the weight in 5 weeks in lewis strain rats (approximately 80 g) as it did in wistar strain rats (approximately 40 g). wistar tumor passaged into lewis rats adopted the accelerated growth rate of the lewis tumor on the second passage. conversely lewis tumor passaged into wistar rats grew like wistar lineage chondrosarcoma after two passages. lewis and wistar tumors had a similar histological appearance. the extracellular matrix composition ...	1987	2952454
defect in b cell function in htlv iii/lav positive hemophilia patients.	the capacity of the peripheral blood lymphocytes (pbl) to generate an antibody response in vitro t cell-dependent antigen ovalbumin was studied in 12 severe hemophilia patients who were otherwise in good health. pbl from four of 12 patients were not capable of generating such a response after stimulation in vitro, whereas all controls were normal. this negative plaque-forming cell (pfc) response coincided with the presence of antibodies directed toward human t-lymphotropic virus iii/lymphadenopa ...	1987	2952185