Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| htlv-iii status and abnormalities in t lymphocyte distribution in children with hemophilia a. | children with hemophilia a are at risk for the acquired immunodeficiency syndrome (aids). clinically asymptomatic hemophiliacs demonstrate many immune abnormalities that might represent exposure to the aids agent through blood products or be a natural reaction to their therapy. in this study, we examined lymphocyte subset distribution in children with hemophilia a who had been exposed to htlv-iii as determined by antibody seroconversion. seroconversion to htlv-iii was confirmed using western blo ... | 1986 | 3006971 |
| half-site editing: an in vitro mutagenesis procedure for truncating a dna fragment and introducing a new restriction site. | half-site editing is an in vitro mutagenesis procedure designed for use in making precise plasmid constructions. like many in vitro mutagenesis techniques, this procedure involves hybridization of a mutagenic oligonucleotide primer to single-stranded template dna followed by polymerization with dna polymerase i (klenow). half-site editing differs from other techniques in two main ways. first, t4 dna polymerase treatment truncates the target dna at a point determined by the primer and repairs any ... | 1986 | 3006543 |
| replicative and cytopathic potential of htlv-iii/lav with sor gene deletions. | the genome of the human t-lymphotropic virus type iii (htlv-iii/lav) has the potential to encode at least three polypeptides in addition to those encoded by the gag, pol, and env genes. in this study, the product of the sor (short open reading frame) region, which overlaps the 3' end of the pol gene, was found to be a protein with a molecular weight of 23,000. an assay was developed for testing the ability of cloned htlv-iii proviruses to produce viruses cytopathic for t4+ lymphocytes. in the ce ... | 1986 | 3006244 |
| bacteriophage t4 dna topoisomerase mediates illegitimate recombination in vitro. | we have found that purified t4 dna topoisomerase promotes recombination between two phage lambda dna molecules in an in vitro system. in this cross, t4 dna topoisomerase alone is able to catalyze the recombination and produce a linear monomer recombinant dna that can be packaged in vitro. atp is not required for this recombination, while oxolinic acid stimulates it. the recombinant dna molecules contain duplications or deletions, and the crossovers take place between nonhomologous and nonspecifi ... | 1986 | 3006033 |
| stabilization of t4 polynucleotide kinase by macromolecular crowding. | t4 polynucleotide kinase rapidly loses activity during its reaction on duplex dna termini. addition of high concentrations of nonspecific polymers reverses or prevents this inactivation. in contrast, additions of related materials of lower molecular weight are relatively ineffective in stabilizing the kinase. such a pattern suggests that the stabilizing effects of polymers on kinase activity are due to macromolecular crowding. an effect of crowding on the known tendency of the kinase to undergo ... | 1986 | 3005982 |
| regulation of thyrotropin secretion by the central epinephrine system. studies in the chronically cannulated rat. | in the present investigation cns epinephrine (epi) biosynthesis was selectively interrupted with specific norepinephrine n-methyltransferase (nmt) inhibitors, sk&f 64139 (smith, kline & french laboratories) and ly 78335 (eli lilly & co. research laboratories), to determine the effects of central epi depletion on basal and cold, thyrotropin-releasing hormone, and hypothalamic somatostatin antiserum induced thyrotropin (tsh) secretion in chronically cannulated rats. because these nmt inhibitors al ... | 1986 | 3005895 |
| specific lysis of varicella zoster virus-infected b lymphoblasts by human t cells. | epstein-barr virus-transformed human b cells expressed cell surface varicella-zoster virus (vzv) antigens after superinfection with vzv although they did not form infectious centers in a plaque assay. the vzv-superinfected cells were lysed by autologous vzv-stimulated t-cell lines and their derivative clones. the effector cells were specific for vzv and an hla dr antigen and were t4+. the specificity of lysis of epstein-barr virus-transformed, vzv-superinfected targets by prestimulated mononucle ... | 1986 | 3005647 |
| persistent infection of chimpanzees with human t-lymphotropic virus type iii/lymphadenopathy-associated virus: a potential model for acquired immunodeficiency syndrome. | the lymphadenopathy-associated virus (lav) prototype strain of human t-lymphotropic virus type iii/lav was transmitted to juvenile chimpanzees with no prior immunostimulation by (i) intravenous injection of autologous cells infected in vitro, (ii) intravenous injection of cell-free virus, and (iii) transfusion from a previously infected chimpanzee. all five animals that received more than one 50% tissue culture infective dose were persistently infected with lav or chimpanzee-passaged lav for up ... | 1986 | 3005641 |
| transferrin synthesis by inducer t lymphocytes. | transferrin (tf) is a growth factor that transports iron in plasma. it is essential for proliferation of activated t lymphocytes. previous studies have suggested that peripheral blood cells are capable of synthesizing tf. using in situ hybridization techniques and human tf complementary dnas as probes, peripheral blood cells have been examined for sites of tf messenger rna (mrna) transcription. the studies described here demonstrate that tf is synthesized by a specific subset of t lymphocytes, t ... | 1986 | 3005367 |
| [studies on the thyrotropic activity of hcg and its derivatives]. | the placenta may be involved in the regulation of the maternal thyroid function during pregnancy. as human chorionic gonadotropin (hcg) is a primary protein from the placenta, we examined the thyrotropic activity of hcg and its derivatives using tissue culture and monolayer cell culture of human thyroid glands. the tsh receptor preparation was made from thyroid tissues, and its binding affinity with hcg and asialo hcg (as-hcg) was examined. hcg did not bind to tsh receptor, but as-hcg did. in th ... | 1985 | 3005061 |
| human t lymphocyte clones specific for malaria (plasmodium falciparum) antigens. | peripheral blood mononuclear cells (pbm) from a patient who had lived in a malarial-endemic area were cultured in the presence of malarial antigens (a lysate of plasmodium-infected erythrocytes). responding cells were grown in il-2-containing medium and then cloned, and subsequently subcloned, in the presence of phytohemagglutinin and allogeneic irradiated pbm. ten clones were specific for malarial antigens. they proliferated in response to p. falciparum extract, but not to a lysate of uninfecte ... | 1985 | 3004971 |
| regulation of thyroxine 5'-deiodinase by thyroid hormones and activators of protein kinase c in gh4c1 cells. | the regulation of t4 5'-deiodinase activity was studied in cultured gh4c1 cells. enzyme activity was measured in cell sonicates as the release of radioiodide from [125i]t4. enzyme activity was stimulated 2- to 3-fold by hypothyroid serum and activators of protein kinase c, such as trh and phorbol esters. the hypothyroid serum effect was maximal by 3 h, whereas trh and phorbol esters required 6 h to achieve a maximal effect. the hypothyroid serum effect was gone within 4 h of returning the cells ... | 1986 | 3004918 |
| thyroxine and 3,5,3'-triiodothyronine bind to the same putative receptor in hepatic nuclei of rana catesbeiana tadpoles. | previous studies have provided conflicting evidence as to whether tadpole liver nuclei contain the same number of high affinity binding sites for t4 as they do for t3. inability to prepare stable tadpole liver nuclei may have contributed to these inconsistencies. thus, a study was carried out in which the in vivo binding of t4 and t3 by tadpole liver nuclei was reexamined with a greatly improved method for isolating liver nuclei. it was found that the maximum binding capacities of the nuclei for ... | 1986 | 3004895 |
| kaposi's sarcoma and htlv-iii infection. virus-like particles in skin lesions: experimental observations. | skin biopsies from a subject affected by ks and aids were examined by means of em. samples were obtained both in correspondence of a typical nodule, and from an apparently normal area. the disease process had started at least 8 mo. before, and the patient showed a reduced number of okt4 cells, with an inversion of the normal t4/t8 ratio. besides that, high titre antibodies versus htlv-iii were present, whereas antibodies versus cmv and ebv were not demonstrable. light and electron microscopy dem ... | 1985 | 3004541 |
| antler cycle and thermolabile and thermostable alkaline phosphatase in white-tailed deer; circannual and circadian rhythms and variation after thyroxine, dexamethasone and acth administration. | the plasma levels of thermolabile (tlap) and thermostable (tsap) alkaline phosphatase were investigated in adult male white-tailed deer. distinct seasonal variation of tlap (with highly elevated levels in july) were observed, whereas tsap exhibits low concentration with a small increase in october. no circadian rhythm was found for tlap or tsap. a close correlation (r = 0.98) between tlap activity and antler weight was found. administration of acth or dexamethasone were ineffective in influencin ... | 1986 | 3004090 |
| [relation between blood levels of thyroid hormones and insulin and lipid metabolism and the clinical course of chronic forms of ischemic heart disease]. | ninety patients aged 41 to 68 years with the chronic patterns of coronary heart disease (chd) were examined for the content of triiodothyronine (t3), total t4 and free thyroxine (ft4), thyrotropic hormone (tth), adrenocorticotropic hormone (acth), and insulin. at the same time the patients were examined with the aid of the glucose tolerance test, determination of blood concentration of cholesterol, triglyceride, high density lipoprotein cholesterol, fibrinogen and soluble fibrin. the patients wi ... | 1985 | 3003947 |
| nononcogenic deletion mutants of herpesvirus saimiri are defective for in vitro immortalization. | herpesvirus saimiri l-dna sequences between 0.0 and 4.0 map units (4.5 kilobase pairs) are required for oncogenicity; these sequences are not required for replication of the virus. to investigate the basis for the lack of oncogenicity of mutants with deletions in this region and to study the function of this region, we developed a reliable system for in vitro immortalization by herpesvirus saimiri. in contrast to peripheral blood lymphocytes from cotton-top tamarins (saguinus oedipus) and owl mo ... | 1986 | 3003401 |
| cyclic amp release from perfused dog thyroid lobes as a sensitive indicator of tsh activation of the secretion process. evidence for two types of thyroid secretion latency. | it has been demonstrated in various types of thyroid tissue preparations that cyclic amp (camp) released into the medium reflects the amount of camp in the cells. in the present study employing perfused dog thyroid lobes the dynamics of camp release were compared to those of thyroxine (t4) and triiodothyronine (t3) release. the experiments gave evidence that even the lowest concentrations of tsh which stimulate hormone release (in this study 1 microu/ml) also activate the camp system; the very h ... | 1985 | 3002933 |
| [the biocellular effect of thyroid hormone on functional differentiation of porcine granulosa cells in culture]. | to elucidate if the thyroid hormone acts directly on the ovary, the biocellular effect of l-thyroxine (t4) on porcine granulosa cells cultured in vitro was investigated. monolayer cultures of porcine granulosa cells obtained from small (1 approximately 2 mm), medium (3 approximately 5 mm) or large (6 approximately 11 mm) follicles were carried out in the presence of porcine fsh (100 ng/ml). concomitant treatment with t4 promoted fsh-dependent morphological luteinization, i.e. alteration of immat ... | 1985 | 3002876 |
| thyroid hormone inhibition of phosphatidylinositol-specific phospholipase c in rat liver. | we investigated the effect of thyroid hormone on phosphatidylinositol-specific phospholipase c activity in rat liver. thyroidectomy increased the activity of the enzyme. thyroid hormone (t4, 40 micrograms) administration to thyroidectomized-rats decreased phospholipase c activity. the inhibition induced by thyroid hormone was of a non-competitive type. the higher concentration of ca2+ strongly inhibited the activity of the enzyme obtained from thyroidectomized-rats' liver in vitro. the diminishe ... | 1985 | 3002770 |
| transferrin receptor bearing cells in the peripheral blood of patients with rheumatoid arthritis. | activated, proliferating lymphocytes are a feature of rheumatoid arthritis. they are present both in the synovial membrane and in the peripheral circulation. the expression of transferrin receptors(tfr) is a good marker of cellular proliferation. this study shows increased levels of circulating tfr-bearing lymphocytes in patients with rheumatoid arthritis (ra). the tfr+ population contains a disproportionately large number of t4+ cells, leading to a high t4:t8 ratio (5:1 in the tfr+ population, ... | 1985 | 3002686 |
| the effect of cytomegalovirus infection on t lymphocytes after allogeneic bone marrow transplantation. | the influence of cytomegalovirus (cmv) infection on peripheral t lymphocyte repopulation was studied in 59 bone marrow transplant (bmt) recipients who received either cyclosporin a (cya) or methotrexate (mtx) as prophylaxis for acute graft-versus-host disease. we used monoclonal antibodies and single- or double-marker immunofluorescence for the quantitation of t4+, t8+ and hnk1+ t cell subpopulations. cmv infection was serologically diagnosed by an enzyme-linked immunosorbent assay (elisa), and ... | 1985 | 3002684 |
| catecholamine, vasoactive intestinal peptide and thyrotrophin-dependent camp levels display a different sensitivity to iodothyronines in both normal and pathological human thyroid cells in culture. | as the interactions of iodothyronines on adrenergic and vipergic receptors are not clear, the effect of exogenous t3 and t4 on catecholamine- and vip-induced camp accumulation in human normal thyroid cells after eight days of primary culture has been investigated. to evaluate the effect of endogenous iodothyronines, the response of the adenylate cyclase system to isoprenaline, adrenaline, vip, and tsh was studied during a 10 d period. t3 and t4 were unable to modify the catecholamine- and vip-in ... | 1985 | 3002668 |
| a stereochemical study of the mechanism of activation of donor oligonucleotides by rna ligase from bacteriophage t4 infected escherichia coli. | rna ligase from bacteriophage t4 infected escherichia coli catalyzes the activation of adenosine 3',5'-bisphosphate (representing the donor oligonucleotide) by adenosine 5'-[(s)-alpha-17o,alpha,alpha-18o2]triphosphate with retention of configuration at p alpha. since single-step enzyme-catalyzed nucleotidyl transfer reactions proceed with inversion, this stereochemical result provides support for a double displacement mechanism involving an adenylyl-enzyme intermediate as proposed previously fro ... | 1985 | 3002453 |
| specific b lymphocytes efficiently pick up, process and present antigen to t cells. | to study the nature of the antigen bridge between specific b and t lymphocytes we have isolated from the same donor clones of human t cells and ebv-transformed b cells specific for tetanus toxoid (tt). we found that tt-specific b cells are extremely efficient in presenting tt (but not an unrelated antigen) to t cells, as they can trigger t cell proliferation in the presence of only 10(-12) m tt, a concentration that is approximately 10(4) times lower than that required for presentation by conven ... | 1985 | 3002320 |
| a putative transforming gene of jijoye virus differs from that of epstein-barr virus prototypes. | the p3hr-1 strain of epstein-barr virus (ebv), a nontransforming clonal derivative of jijoye (ebv), is characterized by a deletion of 6.6 kb involving part of the bamhi-w repeats and the adjacent region including the noti repeats. in the transforming parental jijoye virus this region differs from the corresponding regions in b95-8 or m-aba virus. the hindiii-b fragments which carry this region from both jijoye and prototype m-aba (ebv) viruses have been cloned and subclones have been constructed ... | 1985 | 3002016 |
| morphological and biochemical studies on minocycline-induced black thyroid in rats. | the thyroid glands of the rats treated with 100 mg/kg/day of minocycline for 35 days showed black discoloration. in these thyroids, brown pigment granules were seen in the follicle epithelial cells, and they histochemically stained in the same manner as melanin. by electron microscopy, the deposition of the electron-dense material first occurred in lysosome-like granules and, with further administration, was observed also in the rough endoplasmic reticulum in some cells. thin-layer chromatograph ... | 1985 | 3001973 |
| binding of htlv-iii/lav to t4+ t cells by a complex of the 110k viral protein and the t4 molecule. | human t-lymphotropic virus type iii (htlv-iii) or lymphadenopathy-associated virus (lav) is tropic for human t cells with the helper-inducer phenotype, as defined by reactivity with monoclonal antibodies specific for the t4 molecule. treatment of t4+ t cells with monoclonal antibodies to t4 antigen blocks htlv-iii/lav binding, syncytia formation, and infectivity. thus, it has been inferred that the t4 molecule itself is a virus receptor. in the present studies, the surfaces of t4+ t cells were l ... | 1986 | 3001934 |
| modulation of myocardial l-triiodothyronine receptors in normal, hypothyroid, and hyperthyroid rats. | to characterize the nuclear receptor believed to mediate the thyroid hormones' actions on the heart, binding of l-[125i]t3 to extracts of myocardial cell nuclei from normal, propylthiouracil, and t4-treated rats has been investigated. analysis of in vitro iodothyronine binding to this solubilized nuclear preparation revealed multiple saturable, specific binding sites for t3. high affinity binding for t3 (kd = 4.2 +/- 1.0 x 10(-10) mol/l), and lower affinity (kd approximately 10(-8) mol/l) bindin ... | 1986 | 3001476 |
| endocrine responses of healthy parrots to acth and thyroid stimulating hormone. | effects of exogenous acth on plasma corticosterone and cortisol concentrations and the effects of thyroid stimulating hormone (tsh) on plasma triiodothyronine (t3) and thyroxine (t4) were determined in the following 3 species of parrots: red-lored amazon (group 1), blue-fronted amazon (group 2), and african gray (group 3). each bird was given acth (0.125 mg/bird) im, except for 3 to 4 birds in each group, which were given saline solution (controls). blood samples were collected before and 90 min ... | 1985 | 3000998 |
| thyroid hormone binding to isolated trout (salmo gairdneri) liver nuclei in vitro: binding affinity, capacity, and chemical specificity. | isolated nuclei from trout hepatocytes demonstrated in vitro high-affinity and low-capacity binding for 3,5,3'-tri-iodo-l-thyronine (t3). binding was reversible; the dissociation rate was 5.7 x 10(-3) min-1 at 15 degrees c. linear scatchard plots suggested a single class of noncooperative sites (kd = 1.4 +/- 0.10 x 10(-10) m; maximum binding capacity (mbc) = 62 +/- 10 fmol/mg dna). after correction for site degradation, site occupancy by endogenous t3, and the dissociation rate of endogenous bou ... | 1986 | 3000865 |
| induction of growth hormone (gh) receptors in adipocytes of hypophysectomized rats by gh. | the ability of gh to regulate its own receptor in adipocytes of hormone-substituted hypophysectomized rats was studied. male rats (130-150 g) were hypophysectomized and substituted with t4 and cortisone. a fixed dose of gh (bovine gh, human gh, or ovine gh), was administered for 5-6 days in three different ways: 1) two injections/day, 2) four injections/day, or 3) by osmotic minipumps. gh binding was measured in cell aliquots using [125i]human gh. in unsubstituted hypophysectomized animals, gh b ... | 1986 | 3000727 |
| the stability of bacteriophage t4 gene 32 mrna: a 5' leader sequence that can stabilize mrna transcripts. | in t4-infected cells, the gene 32 monocistronic mrna is very stable. to study the molecular basis for this stability, we have constructed chimeric plasmids containing the monocistronic promoter and the gene 32 translation initiation sequence fused to either most of the e. coli lac operon or only a segment of the lacz gene, followed by the gene 32 transcription terminator. the resulting hybrid transcripts are unstable in uninfected cells. in phage-infected cells, however, the hybrid mrnas are at ... | 1985 | 3000608 |
| lung in acquired immune deficiency syndrome: infectious and immunological status assessed by bronchoalveolar lavage. | bronchoalveolar lavage (bal) has been performed in 63 patients with acquired immune deficiency syndrome (aids) and 20 patients with chronic generalized lymphadenopathy (cgl) for the diagnosis of lung opportunistic infections and analysis of immune effector cells of the lower respiratory tract. in patients with aids, pneumocystis carinii was found in 63%. cytomegalovirus (cmv) pneumonia was assessed by viral cultures of bal fluid and microscopic examination: cmv was found in 62% and 39% respectiv ... | 1985 | 3000489 |
| comparative characterization of thyroid hormone receptors and binding proteins in rat liver nucleus, plasma membrane, and cytosol by photoaffinity labeling with l-thyroxine. | photoaffinity labeling with underivatized thyroxine (t4) was used to identify and compare the t4 binding proteins in rat liver cytosol, nuclear extract, and purified plasma membrane. when these subcellular fractions were incubated with a tracer concentration of [125i]t4, irradiated with light above 300 nm, and individually analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, the radioactivity profiles revealed the presence of t4 binding proteins of molecular masses of 70, 52, 4 ... | 1985 | 3000426 |
| identification of thyroid hormone receptors in rat liver nuclei by photoaffinity labeling with l-thyroxine and triiodo-l-thyronine. | photoaffinity labeling of rat liver nuclear extract with underivatized thyroid hormones was performed after incubation with 1 nm [3',5'-125i]thyroxine ([125i]t4) or [3'-125i]triiodothyronine [( 125i]t3) by irradiation with light above 300 nm. sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the covalently photolabeled nuclear extract revealed four distinct hormone binding proteins of molecular masses 96, 56, 45, and 35 kilodaltons (kda), respectively. distribution of the hormone amon ... | 1985 | 3000425 |
| nucleotide sequence of bacteriophage t4 uvsy gene. | we have determined the nucleotide sequence of a 1001-bp region comprising the uvsy gene of bacteriophage t4. an open reading frame of 420 base pairs was found to encode the uvsy gene product. the uvsy gene comprised a 140-amino acid protein with atg (methionine) as the initiation codon, which is consistent with the molecular weight determined by sds-polyacrylamide gel electrophoresis. the uvsy gene was oriented in the direction of the early genes and a sequence common to the middle promoter cons ... | 1985 | 3000071 |
| the immune system in aids. | recent research suggests that the collapse of the immune system that accompanies acquired immunodeficiency syndrome (aids) stems largely from a single defect: a reduction in the number and a change in the function of the t4 lymphocytes. this knowledge may make it possible to lessen the effects of aids, and eventually to prevent and cure it. the loss of t4 lymphocytes from the blood, lymph nodes, spleen, and other tissues in which they are normally concentrated is a consistent finding in aids ... | 1985 | 2999972 |
| [use of cibacron blue f 3ga-cl-sepharose 6b for the purification of t4 dna- and rna-ligases]. | the sorption capacity of the dye cibacron blue f3ga, immobilized on cl-sepharose 6b and other support matrices, in respect to dna- and rna-ligases t4 was being studies. cibacron blue f3ga immobilized on cl-sepharose 6b binds a three-fold amount of dna-ligase in comparison to rna-ligase. the enzyme chromatography on cibbacron blue f3ga-cl-sepharose 6b revealed a stronger linkage between dna-ligase t4 and the sorbent than between rna-ligase t4 and the sorbent. elution was performed with potassium ... | 1985 | 2999759 |
| t4-induced alpha- and beta-glucosyltransferase: cloning of the genes and a comparison of their products based on sequencing data. | bacteriophage t4 alpha- and beta-glucosyltransferases link glucosyl units to the 5-hmdc residues of its dna. the monoglucosyl group in alpha-linkage predominates over the one in beta linkage. having recently reported on the nucleotide sequence of gene alpha gt (1) we now determined the nucleotide sequence of gene beta gt. the genes were each cloned on a high expression vector under the control of the lambda pl promoter. after thermo-induction the proteins were isolated and purified to homogeneit ... | 1985 | 2999696 |
| [radiotherapy of nasopharyngeal tumor. evaluation of personal results, consequences for irradiation technic]. | in this paper we present our results of radiotherapy of thirty patients with nasopharyngeal tumour from 1960-1983. before therapy, 19% of the cases were diagnosed as a t1 tumour, 57% of the patients showed a t3 or t4 tumour. in 24% of the patients, lymph node metastases could be recognised in pretherapeutic staging, whereas in 5% distant metastases were evident. in more than 50% of the cases tumour recurrence or metastases occurred within one year, in 47% of these patients there was local recurr ... | 1985 | 2999534 |
| t8 and t3 surface glycoproteins in human t-cell mediation of leukocyte adherence inhibition to extracts of autologous cancer. | monoclonal antibodies (mab's) [anti-leu-1, anti-leu-2a (t8), anti-leu-3a (t4), and anti-leu-4 (t3)] were used to elucidate the type of t-cell mediating leukocyte adherence inhibition (lai) and the role of t-cell surface glycoproteins in lai. t8+ (leu-2a+) and t4+ (leu-3a+) subtypes were isolated by panning. t8+ (leu-2a+) cells showed lai to extracts of autologous cancer, whereas the t4+ (leu-3a+) subset had no lai response. moreover, mab to the t8+ (leu-2a+) glycoprotein negated t-cell lai, but ... | 1985 | 2999497 |
| tcgf(il 2)-receptor inducing factor(s). ii. possible role of atl-derived factor (adf) on constitutive il 2 receptor expression of htlv-i(+) t cell lines. | interleukin 2 (il 2) receptor (il 2-r) is constitutively expressed on t cell lines established from the patients with adult t cell leukemia (atl), which is a human t cell leukemia lymphoma virus (htlv-1)(+) t4(+)-leukemia endemic in japan, the united states, and other countries. many of these cell lines continuously produce an acidic lymphokine, atl-derived factor (adf), which preferentially induces the synthesis and expression of il 2-r on a sensitive htlv-1(-) non-t cell line (yt). the induced ... | 1985 | 2999237 |
| omega mutagenesis in gram-negative bacteria: a selectable interposon which is strongly polar in a wide range of bacterial species. | we have used the 2.0-kb dna fragment omega [prentki and krisch, gene 29 (1984) 303-313] to mutagenize in vitro a broad-host-range plasmid carrying the entire meta-cleavage pathway of the pseudomonas putida tol plasmid pww0. the mutant plasmids were subsequently introduced by conjugal mobilization into a variety of gram-negative bacteria. the omega fragment carries a selectable marker (aada+; spcr/smr), which is expressed in all species tested, as well as flanking transcription and translation te ... | 1985 | 2998930 |
| [changes of peripheral mononuclear cell subpopulations in autoimmune thyroid diseases]. | to estimate abnormalities in humoral or cellular immunity that relate to the etiologies of graves' disease (gd) and hashimoto's thyroiditis (ht), peripheral mononuclear cell subpopulations were enumerated by an immunofluorescence technique using monoclonal antibodies. also antithyrotropin receptor antibodies (thyrotropin-binding inhibitor immunoglobulin, tbii) were measured by the radioreceptor assay according to smith's method; and antithyroglobulin antibodies (tgha) and antithyroid microsomal ... | 1985 | 2998893 |
| the early ontogenesis of thyroid hormone receptor in the rat fetus. | we have determined the concentration of thyroid hormone receptor binding sites in nuclear extracts derived from rat fetal organs throughout gestation and the postnatal period. before day 14 of gestation nuclear extracts were obtained from whole fetuses. no receptor binding activity could be detected at day 12 of gestational age, and small amounts were detected at day 13 (maximum binding capacity less than 50 fmol/mg dna). the receptor could be measured in pools of individual organs from day 14 ( ... | 1985 | 2998737 |
| thyrotrophin receptors, tumour radioiodine concentration and thyroglobulin secretion in differentiated thyroid cancers. | tumour radioiodine concentration has been compared with serum thyroglobulin (tg) and, in a few cases, with tumour complement of thyrotrophin receptors in patients with differentiated thyroid carcinoma. all tumours examined possessed tsh receptors. in most the complement was similar to that of normal thyroid tissue although all but one of the tumours had no detectable 131i concentration in vivo even with excess tsh stimulation. elevated serum tg (patient taking t4 in suppressive dose) was general ... | 1985 | 2998424 |
| multiple thyroid hormone binding sites on rat liver nuclear envelopes. | nuclear envelopes relatively free of plasma membrane contamination were isolated from the male rat liver. equilibrium binding of t3 to nuclear envelopes occurred after incubation for 3 h at 20 degrees c. scatchard analysis revealed two classes of binding sites; a high affinity site having a kd of 1.8 nm with a maximum binding capacity of 14.5 pmol/mg protein and a low affinity site having a kd of 152.1 nm with a maximum binding capacity of 346.8 pmol/mg protein. no degradation of the radioligand ... | 1985 | 2998357 |
| immunological studies in a patient with the glucagonoma syndrome. | the glucagonoma syndrome is a rare clinical entity characterized by a glucagon-producing tumor of the pancreas, necrolytic migratory erythema, weight loss and usually decreased glucose tolerance. lately there has been increasing interest in the interaction of peptide hormones and the immune system, implicating a regulatory role of the peptide hormones on immune activation and function. we present a patient with metastasizing glucagonoma and highly elevated plasma levels of glucagon and pancreati ... | 1985 | 2998160 |
| effects of a 72-hour prostacyclin infusion on the hormone levels in patients with obliterative arterial disease. | effects of a 72-h prostacyclin (pgi2) infusion (5 ng/kg/min) on hormone levels were studied in 11 patients (5 males, 6 females) suffering from obliterative arterial disease of the lower extremities. acth, cortisol, tsh, prolactin (prl), gh, lh, fsh, t3, t4, calcitonin, parathyroid hormone (pth), insulin, plasma renin activity (pra), aldosterone and testosterone levels were measured at -15, 0, 30, 120, 240 min and 24, 48, 72 and 96 h after the infusion. during the first 240 min prl and gh levels ... | 1985 | 2998131 |
| phage p22 lysis genes: nucleotide sequences and functional relationships with t4 and lambda genes. | wild-type and amber mutant alleles of the lysis genes of p22 were cloned and sequenced. gene 13 encodes an 11,520-da basic hydrophobic protein that has 89% amino acid homology to lambda s protein. gene 19 encodes a protein that has a small degree of amino acid homology with t4 lysozyme, but we could detect no homology to lambda r or rz proteins. the protein product of gene 19 was purified; its amino terminal amino acid sequence is as predicted by the dna sequence. it starts with a single amino t ... | 1985 | 2998005 |
| t-cell prolymphocytic leukaemia: a clinical and immunological study. | 2 cases of t-cell prolymphocytic leukaemia (t-pll) were investigated for their reactivity with a series of monoclonal antibodies (moabs) as well as for the cytochemical expression and functional activity of the pathological cells. both patients showed morphological (large cells with abundant cytoplasm and eccentric and irregularly shaped nucleus with large and prominent nucleoli) and clinical (high leucocyte count and splenomegaly) features typical of t-pll. the cells from 1 patient expressed a ... | 1985 | 2997908 |
| the t4 lymphocyte in aids. | 1985 | 2997611 | |
| mechanism of action of thyroid hormones. | thyroid hormones have ubiquitous effects and influence the function of most organs. the influences that thyroid hormones have on these diverse functions are primarily mediated through binding of t3 and t4 to specific nuclear receptor sites. the nuclear action of t3 results in organ-specific increases and decreases of specific mrnas, leading to alteration in the level of the corresponding proteins. in addition to the well established nuclear action of t3, effects of thyroid hormone on other sites ... | 1985 | 2997556 |
| improved separation of rhesus monkey lymphocytes with percoll. | the total yield and the proportions of rhesus monkey peripheral blood mononuclear cells (pbmc) with detectable t4, t8, t11 and ia markers that were purified by density gradient centrifugation through solutions of polyvinyl pyrrolidone (percoll) were significantly greater than those found in pbmc suspensions obtained by using ficoll-hypaque. the percentages of recovery of pbmc and of cells expressing the above markers were lowest among pbmc isolated with a ficoll-hypaque mixture of density 1.077 ... | 1985 | 2997545 |
| effects of thyroid hormone on na-k-adenosine triphosphatase activity along the rat nephron. | na-k-adenosine triphosphatase (atpase) activity was determined in individual nephron segments obtained from the kidneys of euthyroid and hypothyroid rats. one group of animals was made hypothyroid by feeding 0.05% aminotriazole (atz) in the diet for 2 weeks. a second group received the same amount of atz plus 500 micrograms/kg body weight of l-thyroxine (t4) given subcutaneously each day for 2 weeks. a third group received the same diet without atz or t4. there was a 57% (p less than 0.01) decre ... | 1985 | 2997354 |
| the effectiveness of oral iodized oil in the treatment and prophylaxis of endemic goiter. | in a longitudinal study carried out for 2 yr in the darfur region, western sudan, 2316 school children received a single dose of 2 capsules of iodized oil (400 mg iodine) orally, and 1161 school children received 1 ml of the same preparation im (475 mg iodine); 2393 school children served as controls. one year after treatment, goiter prevalence was reduced from 67.0% to 36.0% among the children who had received oral iodized oil and from 71.0% to 42.0% in those who received it im. the prevalence ... | 1985 | 2997259 |
| t4 polynucleotide kinase; cloning of the gene (pset) and amplification of its product. | the t4 gene (pset) for polynucleotide kinase (pnk) has been cloned in lambda. induction of a lambda e-w-s-ci857 prophage in which the pset gene can be transcribed from the late lambda promoter, pr1, leads to greater than 100-fold amplification of pnk activity; pnk comprises approximately 7% of the total soluble cell protein. the purified enzyme, as expected, is both a 5'-kinase and a 3'-phosphatase. the amino acid sequence deduced from an open reading frame identified as the pset gene contains a ... | 1985 | 2996886 |
| detection of neocarzinostatin chromophore-deoxyribose adducts as exonuclease-resistant sites in defined-sequence dna. | a 5'-end-labeled dna restriction fragment was treated with the nonprotein chromophore of neocarzinostatin under anoxia in the presence of dithiothreitol, conditions known to maximize formation of chromophore-deoxyribose adducts. under conditions where unmodified dna was digested to completion, chromophore-treated dna was highly resistant to digestion by exonuclease iii plus the 3'----5' exonucleolytic activity of t4 dna polymerase and partially resistant to digestion by exonuclease iii plus snak ... | 1985 | 2996589 |
| immunopathogenesis of the acquired immunodeficiency syndrome. | many abnormalities of humoral and cellular immunity associated with the acquired immunodeficiency syndrome can be explained by the preferential infection of the t4 lymphocyte subset with the etiologic retrovirus. severe alterations in specific t4 functions, such as inadequate immune responsiveness to specific antigen, result in devastating morbidity and mortality. on the basis of accumulated scientific knowledge, we outline the immunopathogenesis of this syndrome. | 1985 | 2996403 |
| lymphadenopathy-associated virus: from molecular biology to pathogenicity. | recent data indicate that the lymphadenopathy-associated virus (lav) is morphologically similar to animal lentiviruses, such as equine infectious anemia and visna viruses. this finding, together with the cross-reactivity of the core proteins of lav with those of the equine infectious anemia virus and a similarity in genome structure and biological properties, allows lav to be placed in the retroviral subfamily of lentivirinae. molecular data indicate a high degree of genetic variation of the vir ... | 1985 | 2996400 |
| molecular cloning and physical mapping of the genome of fish lymphocystis disease virus. | a defined and complete gene library of the fish lymphocystis disease virus (fldv) genome was established. fldv dna was cleaved with ecori, bamhi, ecori/bamhi and ecori/hindiii and the resulting fragments were inserted into the corresponding sites of the pacyc184 or pat153 plasmid vectors using t4 dna ligase. since fldv dna is highly methylated at cpg sequences (darai et al., 1983; wagner et al., 1985), an escherichia coli gc-3 strain was required to amplify the recombinant plasmids harboring the ... | 1985 | 2996221 |
| in vitro growth characteristics of simian t-lymphotropic virus type iii. | the type c retrovirus simian t-lymphotropic virus type iii (stlv-iii) has been isolated recently from immunodeficient macaque monkeys at the new england regional primate research center. the present studies were done to define the in vitro growth characteristics of this agent. stlv-iii replicates efficiently in interleukin 2-dependent t-cell cultures of macaque peripheral blood lymphocytes (pbl), less efficiently in such cultures of human and gibbon pbl, and inefficiently in baboon pbl. no repli ... | 1985 | 2996002 |
| one pyrimidine dimer inactivates expression of a transfected gene in xeroderma pigmentosum cells. | we have developed a host cell reactivation assay of dna repair utilizing uv-treated plasmid vectors. the assay primarily reflects cellular repair of transcriptional activity of damaged dna measured indirectly as enzyme activity of the transfected genes. we studied three plasmids (psv2cat, 5020 base pairs; psv2catsvgpt, 7268 base pairs; and prsvcat, 5027 base pairs) with different sizes and promoters carrying the bacterial cat gene (cat, chloramphenicol acetyltransferase) in a construction that p ... | 1985 | 2995975 |
| pituitary-testicular axis abnormalities in immature male hypothyroid rats. | the pituitary-testicular disturbances which follow the onset of hypothyroidism were studied in immature male wistar rats rendered hypothyroid by treatment with methimazole (mmi) given in drinking water, starting at 40 days of age. half of the animals continued on mmi (mmi group) up to 140 days of age; the remaining rats were withdrawn mmi at 100 days and injected thereafter s.c. with 3 micrograms of t3 daily, during the last 40 days (mmi + t3 group). ten rats were used as controls (c group). hyp ... | 1985 | 2995728 |
| iodine deficiency, hypothyroidism, and endemic goitre in southern tanzania. a survey showing the positive effects of iodised oil injections by tsh determination in dried blood spots. | the ukinga and uwanji regions, located in the southern highlands of tanzania, were studied for the degree of iodine deficiency and the incidence of goitre and hypothyroidism, respectively. a urinary iodine excretion as low as 17.6 +/- 9.3 micrograms/g creatinine was observed in wangama village. the mean goitre prevalence in 27 villages in uwanji ranged between 65 and 96% (n = 3031 schoolchildren). of 681 pregnant women from ukinga 79.6% had goitre. the prevalence of cretinism as estimated on cli ... | 1985 | 2995534 |
| cellular tropism of the human retrovirus htlv-iii/lav. i. role of t cell activation and expression of the t4 antigen. | in cultures of normal human lymphocytes infected with the human retrovirus htlv-iii/lav, detectable cytoplasmic virus appears and then disappears in a proportion (1 to 10%) of cells, followed by release of virus detected by particulate reverse transcriptase activity, virus antigen assay, and infectivity titer. virus infection is associated with loss of detectable t4 antigen on infected cells and, ultimately, complete loss of t4+ cells from the culture. residual non-t4+ cells are not susceptible ... | 1985 | 2995487 |
| bacteriophage t4 dna replication protein 61. cloning of the gene and purification of the expressed protein. | in vitro, a bacteriophage t4 primase composed of t4 61 and 41 proteins, catalyzes the formation of pentaribonucleotides used to initiate dna synthesis on single-stranded dna. we have determined that cells containing a plasmid with the t4 dna from 18.68 to 15.05 map units express an activity that substitutes for authentic 61 protein in vitro in catalyzing primer-dependent dna synthesis with six other t4 dna replication proteins. this result establishes that this region, genetically assigned to ge ... | 1985 | 2995395 |
| bacteriophage t4 dna replication protein 41. cloning of the gene and purification of the expressed protein. | the bacteriophage t4 primase, composed of the t4 proteins 41 and 61, synthesizes pentaribonucleotides used to prime dna synthesis on single-stranded dna in vitro. 41 protein is also a dna helicase that opens dna in the same direction as the growing replication fork. previously, mattson et al. (mattson, t., van houwe, g., bolle, a., selzer, g., and epstein, r. (1977) mol. gen. genet. 154, 319-326) located part of gene 41 on a 3400-base pair ecori fragment of t4 dna (map units 24.3 to 21.15). in t ... | 1985 | 2995394 |
| enalapril: a review of human pharmacology. | enalapril, an orally-active, long-acting, nonsulphydryl angiotensin-converting enzyme (ace) inhibitor, is extensively hydrolysed in vivo to enalaprilat, its bioactive form. bioactivation probably occurs in the liver. metabolism beyond activation to enalaprilat is not observed in man. administration with food does not affect the bioavailability of enalapril; excretion of enalapril and enalaprilat is primarily renal. peak serum enalaprilat concentrations are reached 4 hours post-dose, and the prof ... | 1985 | 2994984 |
| thyroid-dependent maturation of neonatal brain but not lung epidermal growth factor receptors. | although the role of thyroid hormones in enhancing lung and brain maturation during the perinatal period is well established, the cellular mechanisms involved in these processes are incompletely understood. hypothyroidism retards the development of fetal pulmonary insulin, neonatal pulmonary beta-adrenergic and neonatal brain insulin receptors. in this study, we investigated the effect of hypo- or hyperthyroidism on the development of neonatal brain and lung epidermal growth factor (egf) recepto ... | 1985 | 2994970 |
| [effective method of oligonucleotide-controlled mutagenesis of dna fragments]. | a procedure has been designed for changing specific nucleotides in a dna sequence with efficiency. the method involves the use of the specially constructed cloning vectors pbrs1, phs1, and phs2. these plasmids are derivatives of pbr322 in which the ecori-hindiii region has been replaced by synthetic duplexes carrying smai, hpai and xhoi sites, in addition to ecori and hindiii sites. the dna fragment to be mutagenized is cloned in phs1 (or pbrs1, or phs2) using restriction sites close to the smai ... | 1985 | 2994683 |
| conformational microheterogeneity in a dna double helix: structure of restriction endonuclease bam h1 recognition site. | structural studies using 500 mhz 1h nmr spectroscopy on bam h1 recognition site d(ggatcc)2 in solution at 19 degrees is reported. the resonances from the sugar ring and base protons have been assigned from the 2d-cosy and noesy spectra. analyses of the noesy cross-peaks between the base protons h8/h6 and sugar protons h2'/h2", h3' reveal that the nucleotide units g2, a3 and c6 adopt (c3'-endo, chi = 200 degrees-220 degrees) conformation while g1, t4 and c5 exhibit (c2'-endo, chi = 240 degrees-26 ... | 1985 | 2994650 |
| angiotensin-converting enzyme activity. a potential marker of tissue hypothyroidism in critical illness. | we measured serum angiotensin-converting enzyme (ace) activity radiometrically as a possible indicator of reduced thyroid function in 57 euthyroid controls, 27 patients in a noncardiac intensive care unit (13 with medical and 14 with surgical disorders), and 29 patients having coronary artery bypass grafting. in the last group, blood was obtained preoperatively and one day and one month after surgery (group 1; n = 18) or preoperatively and six hours and one day after surgery (group 2; n = 11). p ... | 1985 | 2994585 |
| epstein-barr virus infection in a child with acquired immunodeficiency syndrome. | a 3-year-old girl, born to an intravenous-drug-dependent mother, had protracted diarrhea, failure to thrive, generalized lymphadenopathy, and recurrent fevers during the first six months of life. at 7 months of age, the epstein-barr virus (ebv) genome was detected in her saliva by dna dot-blot hybridization using a cloned ebv probe. spontaneous ebv+ lymphoblastoid cell lines had repeatedly developed from her peripheral blood lymphocytes over the subsequent 2 1/2 years. at 11 months of age, persi ... | 1985 | 2994462 |
| squamous cell carcinoma of the nasopharynx. an analysis of failure patterns after radiation therapy. | seventy-seven patients with nasopharyngeal squamous cell carcinoma were treated with irradiation, with or without chemotherapy. sixty-three (82%) developed a relapse in some part of the body; the first relapse appeared at primary, cervical and distant sites in 45 (71%), 30 (48%) and 12 (19%) of the 63 relapsing patients, respectively. in 22 of the 63 relapsing patients, the first relapse occurred simultaneously in two or more sites. local recurrence-free survival was higher for the t1 + t2 group ... | 1985 | 2994387 |
| beta receptors in peripheral mononuclear cells increase acutely during exercise. | five healthy adult well trained men (averaging 49 miles running per week) were exercised to exhaustion on a treadmill. plasma epinephrine increased from 168 +/- 37 pg/ml prior to exercise to 633 +/- 155 pg/ml immediately after exercise (p less than 0.025) and plasma norepinephrine increased from 1608 +/- 345 to 8576 +/- 1662 pg/ml (p less than 0.025) in the two respective periods. beta receptor density increased from 53 +/- 18 fmol/mg protein before exercise, to 223 +/- 63 fmol/mg (p less than 0 ... | 1985 | 2994334 |
| persistent noncytopathic infection of normal human t lymphocytes with aids-associated retrovirus. | infection of normal peripheral blood t cells by the acquired immune deficiency syndrome (aids)-associated retrovirus (arv) was evaluated in long-term cultures of helper-inducer t cells (t4 cells). cells that were inoculated with arv and maintained in medium supplemented with interleukin-2 remained productively infected with this virus for more than 4 months in culture, although they showed no cytopathic effects characteristic of acute arv infection. the presence of replicating virus was demonstr ... | 1985 | 2994222 |
| analysis of a region in plasmid r386 containing two functional replicons. | a miniplasmid has been obtained from r386 by ligating ecori fragments with a fragment carrying a kanamycin-resistance gene. it contains a 6.8-kb eco fragment of r386 which hybridizes strongly with several incfi plasmid dnas but not with the primary or secondary replicons of the f plasmid. this mini-r386 is incompatible with certain incfi plasmids, and it appears to be one example of a previously unidentified replicon widely distributed in the incfi group. a region of r386 not closely linked to t ... | 1985 | 2994129 |
| rearrangements of genes for the antigen receptor on t cells as markers of lineage and clonality in human lymphoid neoplasms. | the t alpha and t beta chains of the heterodimeric t-lymphocyte antigen receptor are encoded by separated dna segments that recombine during t-cell development. we have used rearrangements of the t beta gene as a widely applicable marker of clonality in the t-cell lineage. we show that the t beta genes are used in both the t8 and t4 subpopulations of normal t cells and that sézary leukemia, adult t-cell leukemia, and the non-b-lineage acute lymphoblastic leukemias are clonal expansions of t cell ... | 1985 | 2993886 |
| [the region of phage t4 w-29 genes: cloning and expression]. | the ecori fragment of t4 dna containing the w-29 genes and its subfragments were cloned in the pbr322 and the singlestranded m13 phage. hybridization with a cloned dna showed that in t4 infected cells the transcription of the late genes 25-29 depends on the phage-induced rna polymerase changes and on replication of phage dna. at a late infection stage one also observes an enhanced transcription of the (early) genes uvsw and uvsy, which depends on viral dna replication. both early and late genes ... | 1985 | 2993856 |
| effect of cyclosporin a on natural killer cells' response during viral infections. | in the this study the modification of lymphocyte subsets (t3, t4, t8) and natural killer (nk) cells in organ transplanted patients treated with cyclosporin a (cya) in the course of viral infection, have been analyzed. different subsets have been studied with the monoclonal antibody method and infective processes have been verified by serological data of seroconversion. our study has shown that cya at the adopted doses does not alter nk response to viral infection; in fact, in patients with seroc ... | 1985 | 2993825 |
| dna homology and adsorption specificity of pseudomonas aeruginosa virulent bacteriophages. | the dna homology and adsorption specificity of newly isolated virulent bacteriophages of p. aeruginosa have been studied. on the basis of this analysis all phages were divided into four groups: phi k, phi m, phi mnp78-like and phi mnf82-like bacteriophages. dna's of phi k as well as phi m phages were shown to possess different restriction patterns although they have an extensive homology. unlike other groups, phi k phages were characterized by the presence of t4 dna ligase--repaired, single-chai ... | 1985 | 2993807 |
| presence of dna, encoding parts of bacteriophage tail fiber genes, in the chromosome of escherichia coli k-12. | the classical t-even bacteriophages recognize host cells with their long tail fibers. gene products 35, 36, and 37 constitute the distal moiety of these fibers. the free ends of the tail fibers, which are formed by the co2h terminus of gene product 37, possess the host range determinants. it was found that 4 out of 10 different strains of escherichia coli k-12 contained regions of chromosomal dna which hybridized with a probe consisting of genes 35, 36, and 37 of the t-even phage k3. from one st ... | 1985 | 2993246 |
| prognostic value of thyroid hormone levels in patients evaluated for liver transplantation. | the thyroid hormones t4, t3, rt3 and tsh were assayed in 134 adult patients evaluated and accepted as potential liver transplant candidates at the university of pittsburgh from march, 1981 to december, 1983. the subsequent course of these patients was evaluated with respect to the levels of these hormones obtained at the time of acceptance for transplantation. t4 levels were increased significantly while their t3 levels were reduced (both p less than 0.01) in those who survived and were discharg ... | 1985 | 2993148 |
| multisite inhibition by ipodate of iodothyronine secretion from perfused dog thyroid lobes. | cholecystographic radiocontrast agents interfere with thyroid hormones in several ways. in the present study 1 mm ipodate induced a rapid sustained and reversible inhibition of the secretion of t4, t3, rt3, 3,3'-diiodothyronine, and 3',5'-diiodothyronine from perfused dog thyroid lobes. this effect was not reproduced by infusion of 3 mm iodide and not affected by 2 mm methimazol or 2 mm perchlorate. one millimolar of ipodate inhibited secretion of t4 to 23.7 +/- 2.8% of control (+/- se, n = 6), ... | 1985 | 2992917 |
| cellular targets of antilymphocyte antibodies in aids and las. | many patients with acquired immune deficiency syndrome (aids) and lymphadenopathy syndrome (las) have serum antilymphocyte antibodies. the targets of these antibodies are neither sex nor hla directed. there is disagreement about the cell subset at risk. we examined the cellular specificity of antilymphocyte antibodies from aids and las patients by microcytotoxicity on positively selected lymphocytes, by using double-labeling immunofluorescence, and by using well-characterized continuous cell lin ... | 1985 | 2992856 |
| isolation of a lymphadenopathy-associated virus from a patient with the acquired immune deficiency syndrome. | a virus similar to the lymphadenopathy-associated virus or human t-lymphotropic virus type iii, which has been described in association with the acquired immune deficiency syndrome (aids) by several laboratories elsewhere in the world, was isolated from a cape town patient with lymphadenopathy and acquired immune deficiency. this virus has the characteristic morphogenesis and ultrastructure and its genome encodes the virus-specific p24 protein. it is t-lymphotropic and produces the characteristi ... | 1985 | 2992103 |
| isolation of highly purified lymphocyte subsets for functional studies by means of an indirect rosette technique. | an indirect rosette assay, utilizing ox erythrocytes (rbc) coupled with rabbit anti-mouse igg and lymphocytes sensitized with monoclonal mouse antibodies against membrane markers, was used for purification of lymphocyte subsets that were functionally intact. either peripheral blood mononuclear cells (pbmc) or t lymphocytes isolated by sheep rbc rosetting could be used as starting material for obtaining pure t-cell subsets (t4 or t8). the following steps of the method were evaluated: the procedur ... | 1985 | 2992071 |
| expression of a cloned denv gene of bacteriophage t4 in escherichia coli. | a 713-base-pair hae iii fragment from bacteriophage t4 encompassing the denv gene with its preceding promoter has been cloned in a pbr322-derived positive-selection vector and introduced into a variety of dna repair-deficient uvr and rec and uvr,rec escherichia coli strains. the denv gene was found to be expressed, probably from its own promoter, causing pyrimidine dimer incision-deficient uvra, uvrb, uvrc strains to be rescued by the denv gene. a uvrd (dna helicase ii) strain was also complemen ... | 1985 | 2991891 |
| [lymphography in cancer of the prostate]. | the frequency and area of lymphogenic metastasis with relation to the histological structure, differentiation and degree of local dissemination of a primary tumor were determined at lymphography of 114 patients with prostatic cancer. lymph node involvement was often observed in poorly differentiated and solid trabecular adenocarcinoma, scirrhous cancer and locally disseminated tumors extending beyond the prostatic capsule. metastases were usually located in the external and common inguinal lymph ... | 1985 | 2991695 |
| binding of thyroid hormones by nuclei of target tissues during the chick embryo development. | in the present studies we have compared the ontogeny of the binding of thyroxine (t4) and triiodothyronine (t3) to isolated nuclei from various target tissues of chick embryo. we observed a marked difference between the patterns of satchard plots, maximal binding capacities (mbc) and association constants (ka) of t4 and those of t3. scatchard plots revealed that t4 and t3 had different binding sites. in liver, brain and lung mbcs and kas of t3 and t4 were rather similar at day 9, but during the ... | 1985 | 2991675 |
| relationship between bacteriophage t4 and t6 dna topoisomerases. t6 39-protein subunit is equivalent to the combined t4 39- and 60-protein subunits. | t6 dna topoisomerase has been purified from bacteriophage t6 infected escherichia coli. unlike the t4 dna topoisomerase which has three subunits, it consists of two subunits of molecular weights 75,000 and 51,000. they are the products of t6 genes 39 and 52, respectively. the purified t6 enzyme can stimulate in vitro t6 dna replication. it has an atp-dependent dna relaxation activity similar to the t4 enzyme. either atp or datp can be used in both reactions. using a "western blotting" and radioi ... | 1985 | 2991231 |
| thyroid function in adult rough-skinned newts (taricha granulosa): effects of hypophysectomy and hormone replacement. | this study examines the regulation of thyroid activity in adult rough-skinned newts, taricha granulosa. aquatic, breeding-condition newts were hypophysectomized and were injected for 23 days with the mammalian adenohypophysial hormones: acth, prl, and gh. during a fourth week of treatment, these newts also received tsh. plasma t4 concentrations were lower in hypophysectomized newts than in sham-hypophysectomized newts 23 days after surgery. both acth and prl increased t4 titers above these in sa ... | 1985 | 2991080 |
| putative nuclear triiodothyronine receptors in tadpole liver during metamorphic climax. | it has been shown previously that the maximum binding capacity (mbc) of the putative t3 receptors in tadpole red blood cells (rbcs) is increased during development and can be stimulated by treatment with thyroid hormone (th). the present study was performed to determine if the mbc of tadpole liver nuclei is also increased during development or after treatment with th. because of the relatively high levels of endogenous th in tadpoles during climax, the use of an in vivo saturation assay employin ... | 1985 | 2990877 |
| the isolation and nucleotide sequence of a cdna encoding the t cell surface protein t4: a new member of the immunoglobulin gene family. | the surface glycoproteins t4 and t8 define different functional subsets of t lymphocytes and may act as recognition molecules mediating appropriate interactions between the t cell and its target. previously we employed gene transfer and subtractive hybridization to isolate a t8 cdna; now we have isolated and sequenced a cdna clone encoding the t4 molecule. the deduced protein sequence reveals that t4 is an integral membrane protein that shares significant amino acid and structural homologies wit ... | 1985 | 2990730 |
| htlv-iii infection in homosexuals and hemophiliacs in sweden. | two hundred and three homosexual (hs) men and 114 hemophiliacs in sweden were examined for serum antibodies to human t-lymphotropic virus type iii (htlv-iii) and for alterations of t-lymphocyte subsets. sera were screened for htlv-iii antibodies by an enzyme-linked immunosorbent assay and/or a dot immunobinding assay, and positive reactions were confirmed by western blotting. htlv-iii antibodies were demonstrated in 13 of 13 (100%) hs men with acquired immune deficiency syndrome, in 63 of 67 (94 ... | 1985 | 2990693 |
| retroviruses associated with leukemia and ablative syndromes in animals and in human beings. | t-lymphotropic retroviruses of cats cause lymphopenia and immunosuppression and represent the major cause of death in that species. similarly htlv-i which is t4 tropic is associated with an increased risk for development of infectious disease in regions where the virus is endemic. since htlv-i is also believed to be transmitted by blood and by sexual intercourse we considered the possibility that a variant form of htlv might cause aids. the identification of cross-reactive antibodies to htlv-i-m ... | 1985 | 2990682 |
| effect of thyroxine on hepatic glucose-6-phosphatase activity and glycogen content of toad (bufo melanostictus) and lata fish (ophicephalus punctatus) at different stages of life. | the effects of thyroxine (t4) on hepatic glucose-6-phosphatase activity and glycogen content in toad (bufo melanostictus) and lata fish (ophicephalus punctatus) were studied in order to show the difference, if any in the enzyme activity and glycogen metabolism in their liver. thyroxine injections (1 microgram/g) for five consecutive days caused a reduction in hepatic glucose-6-phosphatase activity and glycogen content in toads of immature, juvenile and adult stages. in contrast, lata fish of dif ... | 1985 | 2990159 |
| revision of the case definition of acquired immunodeficiency syndrome for national reporting--united states. | the conference of state and territorial epidemiologists (cste) approved the following definitions regarding the case definition of acquired immunodeficiency syndrome (aids) at its annual meeting in june 1985. 1st, the case definition of aids used for national reporting will continue to include only the more severe manifestations of human t-lymphotropic virus type iii (htlv-iii) infection. 2nd, centers for disease control (cdc) will develop more inclusive definitions and classifications of htlv ... | 1985 | 2989677 |