Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| alterations in the hypothalamic-pituitary-thyroid axis after prolonged residence in antarctica. | the human population which lives and works in polar environments has been increasing steadily over the last 15 years. very little is known about how these residents adjust to their environment. cold adaptation in man is a poorly understood phenomenon. euthermic mammals maintain body temperature during cold exposure via non-shivering thermogenesis, a process which is hormonally mediated. we studied prospectively the response of the hypothalamic-pituitary-thyroid axis in 17 euthyroid men before, d ... | 1986 | 3098460 |
| growth hormone responses to grf 1-29 in patients with primary hypothyroidism before and during replacement therapy with thyroxine. | it is well known that hypothyroidism is frequently associated with impaired gh responses to different stimuli. in the present study we have evaluated gh responses to gh-releasing factor (grf) in patients with primary hypothyroidism before and during t4 replacement therapy. fourteen patients (age range 26-60 years) underwent two grf tests (1 microgram/kg) before and during replacement therapy (150 micrograms/d). administration of t4 increased peak gh responses to grf in 9 patients and in the grou ... | 1986 | 3098458 |
| hypothalamic hypopituitarism following cranial irradiation for nasopharyngeal carcinoma. | eight patients, one male and seven females, with no pre-existing hypothalamic-pituitary disease, who developed symptoms of hypopituitarism following cranial irradiation for nasopharyngeal carcinoma were studied 5 years or more after radiotherapy. all were gh deficient. four of the patients with no gh response during insulin tolerance tests (itt) showed increased gh in response to synthetic human growth hormone releasing factor (grf-44). four patients had impaired cortisol responses to itt, and g ... | 1986 | 3098456 |
| 3,5-diiodotyrosine and thyronine in the urine of patients with chronic renal disease. | urinary 3,5-diiodotyrosine (dit) and thyronine (t0) excretion was investigated in 18 patients with chronic renal disease. in accord with previous findings serum t4 and thyroid hormone binding proteins measured in 17 patients were in the low or normal range. urinary albumin excretion was elevated in all 18 and t4 binding prealbumin (tbpa) in 15 of the 18. urinary t0 excretion measured in 12 patients was also significantly lower than normal (mean +/- sd 4.4 +/- 2.6 vs 15.8 +/- 5.8 nmol/24 h renal ... | 1986 | 3098453 |
| serum thyroid hormones and thyroid hormone binding proteins in patients with completed stroke. | thirty euthyroid patients hospitalized for completed stroke had serum thyroxine, triiodothyronine, thyroxine-binding globulin and thyroxine-binding prealbumin levels significantly decreased (p less than 0.01 to p less than 0.001) compared with 70 age- and sex-matched healthy controls. admission serum thyroxine levels gave the best correlation with mortality (t4 less than 65 nmol/l = 100% mortality; 65-77 nmol/l = 38%; 78-90 nmol/l = 9%; greater than 90 nmol/l = 0%). in general, during hospitaliz ... | 1986 | 3098157 |
| changes in blood glucose, liver glycogen, ketone bodies and plasma insulin in suckling rats treated with a single high cortisol dose one day after birth. | rats treated with cortisol, 0.5 mg one day after birth, show a decrease in body and organ weight and a decrease in pituitary tsh and gh when compared to controls together with a lack of response of tsh to trh. these alterations have been previously found in animals treated with large doses of thyroxine, neo-t4 syndrome, in both populations a brain underdevelopment has been observed. plasma insulin in cortisol-treated animals was higher (p less than 0.01) than in the controls at 8 and 12 days of ... | 1986 | 3098019 |
| decreased serum thyroglobulin levels in the late stage of pregnancy. | serum thyroglobulin levels were serially measured in 25 normal pregnant women to evaluate thyroidal activity during normal pregnancy. measurements included serum t3, t4, free t4, tbg, and tsh. tg and ft4 levels were found to be decreased in the third trimester when compared with those of the first trimester and with those of normal non-pregnant individuals (p less than 0.01). tsh levels were higher than normal in pregnant women at all stages of pregnancy, with a significant rise at the third tri ... | 1986 | 3098016 |
| [biochemical, hematologic, immunologic and cytogenetic study of papillary dermatitis in fattened pigs]. | examinations were performed of 31 fattened pigs suffering from papular dermatitis (pd) and 33 fattened pigs without dermal changes (c) coming from the d. large pig-fattening farm and the production breeding herd of pigs. the weight of the pigs was from 95 to 105 kg. both farms are sited in a mining area (intensive lignite extraction). the pd pigs, compared with the controls (c), had less total protein, cholesterol and calcium in their blood serum and increased concentrations of thyroxine (t4), n ... | 1986 | 3097911 |
| octapeptides deduced from the neuropeptide receptor-like pattern of antigen t4 in brain potently inhibit human immunodeficiency virus receptor binding and t-cell infectivity. | the differentiation antigen t4, present on the helper/inducer subset of t lymphocytes, is thought to serve as the receptor for the human immunodeficiency virus (hiv). we find that a 60-kda protein, immunoprecipitable by monoclonal antibody (mab) okt4, is present on membranes from human brain as well as human t cells. furthermore, the radioiodinated hiv envelope glycoprotein [125i-labeled gp120 (125i-gp120)] can be specifically covalently affixed to a molecule present on rat, monkey, and human br ... | 1986 | 3097649 |
| human interferon alpha and gamma production by lymphocytes during the generation of influenza virus-specific cytotoxic t lymphocytes. | we analysed the production of interferons (ifn)-alpha and -gamma during the generation of human influenza-virus specific cytotoxic t lymphocyte (ctl) responses using monoclonal antibodies in a specific radioimmunoassay. the results showed that the peripheral blood mononuclear cells (pbm) of all donors tested produced ifn-gamma and had influenza a virus-specific ctl activity after stimulation. the amount of ifn-gamma produced and the level of ctl activity were significantly correlated. the pbm of ... | 1986 | 3097247 |
| effects of intravenous trh on growth hormone and cortisol serum levels in children and adolescents with insulin dependent diabetes mellitus. | the effects of an iv thyrotropin releasing hormone (trh) bolus on serum growth hormone (gh) and cortisol levels were evaluated in 59 children and adolescents with insulin dependent diabetes mellitus (iddm) and in 24 healthy, age-matched control subjects. in the iddm group gh baseline levels sharply rose within 30 min after trh and successively normalized. on the contrary, trh injection failed to affect gh serum concentrations in the control group. the gh increase after trh in iddm patients was p ... | 1986 | 3097114 |
| reduced affinity for thyroxine in two of three structural thyroxine-binding prealbumin variants associated with familial amyloidotic polyneuropathy. | t4-binding prealbumin (tbpa), a protein synthesized by the liver, circulates as a tetramer and transports 15-20% of t4. we studied 3 variants of the tbpa monomer recently identified in serum and amyloid fibrils of patients affected by familial amyloidotic polyneuropathy (fap). they represent single amino acid substitutions at positions 30 (type i), 60 (appalachian), and 84 (type ii). tests of thyroid function and the apparent association constant (ka) of t4 binding to tbpa were measured in whole ... | 1986 | 3097057 |
| genetic control of the production of anti-thyroid hormone antibodies in mice immunized with human thyroglobulin. | various strains of mice with different h-2 and igh-i allotypes were immunized with human thyroglobulin (htg) and genetic control of the production of anti-thyroid hormone antibodies was examined. anti-htg antisera obtained from different strains of mice were examined for the presence of anti-thyroid hormone antibodies. at least one ir-gene which controls the production of anti-t4 antibodies by immunization with htg was identified in the i-a subregion. the presence of ir-genes outside the h-2 was ... | 1986 | 3096882 |
| phenotypic characterization of individual interferon-gamma-producing cells after okt3 antibody activation. | this is the first study using a direct approach to determine the phenotype of interferon gamma (ifn-gamma)-producing cells after polyclonal t cell activation. blood mononuclear cells (mnc) were cultured and stimulated by okt3 antibody to produce ifn-gamma. they were then stained with a panel of monoclonal antibodies with specificity for cell surface antigens, fixed and permeabilized in suspension and stained for cytoplasmic ifn-gamma using monoclonal antibodies and indirect immunofluorescence te ... | 1986 | 3096747 |
| discrepancy between uptake of serum triiodothyronine as measured by albumin and charcoal methods in nonthyroidal illnesses. | triiodothyronine (t3) uptake as determined with albumin (at3u) and with charcoal (ct3u) as binders were compared for patients with low-t3 syndrome. both t3u values were identical in normal subjects and in patients with thyroidal disorders, pregnancy, low thyroxin-binding globulin (tbg), or acute hepatitis with high concentration of tbg. in the low-t3 syndrome, at3u was significantly higher than ct3u (38.5 +/- 7.2% vs 29.4 +/- 4.9%, mean +/- sd, n = 37, p less than 0.001), and this difference inc ... | 1986 | 3096613 |
| human gamma interferon enhances release from phytohemagglutinin-stimulated t4+ lymphocytes of activities that stimulate colony formation by granulocyte-macrophage, erythroid, and multipotential progenitor cells. | human gamma interferon (huifn gamma) was evaluated for its effects on the release from human peripheral blood t lymphocytes (greater than 98% pure) stimulated by phytohemagglutinin (pha) of activities that can stimulate granulocyte-macrophage (cfu-gm) colonies and clusters, erythroid (bfu-e) bursts, and mixed (cfu-gemm) colonies. t lymphocytes did not release these activities in the absence of pha with or without huifn gamma. in the presence of pha, pure natural huifn gamma at concentrations of ... | 1986 | 3096401 |
| single-strand binding proteins from phage t4 and e. coli form higher order structures with poly(dt). | complexes of poly(dt) with gene 32 protein from phage t4 or e. coli single-strand binding protein were digested by nuclease p1 from penicillum citrinum. protected fragments were analyzed by gel electrophoresis. in both cases, a series of bands was obtained corresponding to multiples of a repeat unit whose size was about 80 nucleotides. such protected fragments could not be detected under the same experimental conditions when poly(da) was used instead of poly(dt). the formation of nucleosome-like ... | 1986 | 3096391 |
| juvenile thyrotoxicosis. | the incidence of juvenile thyrotoxicosis has not exceeded one case per 100,000 population per year in hungary during the past decades. from more than 200 simultaneous determinations of serum thyroid hormones (t3, t4, ft4) it was concluded that increased ft4 concentrations are the most frequent findings in thyrotoxicosis, even when both or one of the other thyroid hormone concentrations (t3, t4) are normal. tsh and prolactin responses to trh were studied in 18 patients; tsh remained always undete ... | 1986 | 3096041 |
| thyroid function in prematures with respiratory distress syndrome (rds). | reports on thyroid function in newborns with respiratory distress syndrome (rds) are controversial, the significance of obtained results is not clear. therefore we conducted a longitudinal study of thyroid function in 35 infants with rds (gestational age 24-36 weeks, birth weights 650-2770 g). 43 well prematures, matched for gestational age, served as controls. no significant differences were observed in cord blood tsh, t3, t4, tbg values and t4:tbg ratios between infants with and without rds. p ... | 1986 | 3096040 |
| alterations in t4 (cd4) protein and mrna synthesis in cells infected with hiv. | cells infected with the human immunodeficiency virus (hiv) show decreased expression of the 58-kilodalton t4 (cd4) antigen on their surface. in this study, the effect of hiv infection on the synthesis of t4 messenger rna (mrna) and protein products was evaluated in t-cell lines. metabolically labeled lysates from the t4+ cell line sup-t1 were immunoprecipitated with monoclonal antibodies to t4. compared with uninfected cells, hiv-infected sup-t1 cells showed decreased amounts of t4 that coprecip ... | 1986 | 3095925 |
| the effect of double filtration plasmapheresis on thyroid hormone economy and thyroid function. | five cases of rheumatoid arthritis (ra), two cases of systemic lupus erythematosus (sle), a case of mixed connective tissue disease (mctd), and a case of cold urticaria were treated with double filtration plasmapheresis (dfp). each aliquot of plasma was obtained at three different points of the dep circuit during the treatment and concentrations of thyroid hormones as well as thyroxine binding globulin (tbg) were measured. despite the removal of considerable amounts of triiodothyronine (t3), thy ... | 1986 | 3095574 |
| diagnostic value of free triiodothyronine in serum. | serum free t3 concentration has been assessed in various thyroid conditions by a t3 analog method and the results compared with those obtained by equilibrium dialysis in the same individuals. the methodology is easy to perform and reproducible. ft3 determination appears to be especially valuable in detecting borderline thyrotoxicosis as in cases previously cured from thyrotoxicosis but suspected of relapse, or in nontoxic goitrous patients overtreated with t4. | 1986 | 3095510 |
| the immunoregulatory nature of iron. ii. lymphocyte surface marker expression. | previously, we presented preliminary evidence that supported our hypothesis for the immunoregulatory nature of iron [7]. the objective of the present work was to test that hypothesis in greater detail. our approach was to examine the effect that iron had on the expression of the surface markers on lymphocytes that had been activated by pokeweed mitogen (pwm). the two categories of lymphoid surface molecules were enumerated on those cells; first were those that identify t lymphocytes and second, ... | 1986 | 3095475 |
| accessory cell-t cell interactions involved in anti-cd3-induced t4 and t8 cell proliferation: analysis with monoclonal antibodies. | the effect of monoclonal antibodies (mab) directed at t cell and accessory cell (ac) surface molecules on okt3-induced t4 and t8 cell proliferation was examined. mab directed at nonpolymorphic class i (w6/32, mb40.5) and class ii (l243) major histocompatibility complex (mhc)-encoded gene products, an epitope common to lfa-1, cr3, and the p150, 95 molecule (60.3), and a heterodimer present on monocytes (m phi) and activated t cells (4f2) inhibited m phi-supported okt3-induced proliferation of bot ... | 1986 | 3095421 |
| inhibition of follicle-stimulating hormone induction of aromatase activity in porcine granulosa cells by thyroxine and triiodothyronine. | we examined the effects of t3 and t4 on basal and fsh-induced aromatase activity in granulosa cells isolated from medium-sized follicles (4-6 mm) of prepubertal pigs. treatment of cells with t3 or t4 alone during an initial 48-h induction period did not result in any significant change in aromatase activity, as measured by 17 beta-estradiol accumulation during the subsequent 6-h test period, when testosterone (0.5 microm) was added as substrate. however, when cells were cultured with fsh and t3 ... | 1986 | 3095107 |
| the t4 gene encodes the aids virus receptor and is expressed in the immune system and the brain. | the isolation of clones encoding the human surface protein t4, and the expression of the t4 gene in new cellular environments, have enabled us to examine the role of this protein in the pathogenesis of aids. our studies support a mechanism of aids virus infection that initially involves the specific interaction of the aids virus with t4 molecules on the cell surface. this association can be demonstrated on t4+ transformed t and b lymphocytes as well as epithelial cells. furthermore, the presence ... | 1986 | 3094962 |
| [value of the triiodothyronine suppression test with serum t4 and thyroid 131i uptake as indexes in judging the prognosis of graves' disease]. | 1986 | 3094869 | |
| pituitary-thyroid function and thyrotropin, prolactin and growth hormone responses to trh in patients with chronic alcoholism. | basal plasma concentrations of thyroxine (t4), 3,3',5-triiodothyronine (t3), free t4 index (tf4i), free t3 index (ft3i) reverse t3, 3,3',5-triiodothyronine (rt3), resin t3 uptake (tr3u), thyroxine-binding globulin (tbg), thyrotropin (tsh), prolactin (prl) and growth hormone (gh) as well as thyrotropin releasing hormone (trh) stimulated tsh, prl and gh were investigated in 31 consecutive male patients (mean age 41 years) with chronic alcoholism. according to the histology of their liver biopsies ... | 1986 | 3094323 |
| [prealbumin-associated hyperthyroxinemia]. | prealbumin associated hyperthyroxinaemia represents a rare but important protein binding anomaly, causing difficulties in differential diagnostics. this anomaly is based on a variant prealbumin showing an increased affinity to t4 and rt3 and in some cases also when using t4 analogue tracers. the condition of the patient is clinically euthyroid which is confirmed by a normal btsh level, a normal response to trh, and peripheral parameters (bmr, asr, sti, shbg) in the euthyroid range. this paper de ... | 1986 | 3094315 |
| immune responses during human schistosomiasis mansoni. xiii. immunological status of spleen cells from hospital patients with hepatosplenic disease. | splenocytes from 25 patients with severe hepatosplenic schistosomiasis mansoni were obtained after therapeutic splenectomy. spleen cells were phenotyped and analysed for responsiveness to mitogens or heterogeneous schistosome-derived antigenic preparations (eggs, sea; adult worms, swap; cercariae, cerc) in blastogenesis assays and lymphokine production systems, and were compared with peripheral blood mononuclear cells (pbmn). splenic lymphocytes were 55% t lymphocytes (sheep erythrocyte rosette- ... | 1986 | 3094136 |
| phenotypic identification of mononuclear cells in oral premalignant lesions and cancer by monoclonal antibodies. | to explore the nature and importance of mononuclear cells of different phenotypes in oral premalignant lesions and oral cancer, we studied biopsy specimens from 21 oral red and/or white lesions (6 hyperkeratosis, 3 mild dysplasia, 4 severe dysplasia and 8 squamous cell carcinoma), using monoclonal antibodies and avidin-biotin-peroxidase complex staining. peripheral blood samples (pb) from 4 normal subjects and 5 reactive lymph nodes (ln) were used as controls for the technique. t11-positive cell ... | 1986 | 3093654 |
| inherited heat-stable variant thyroxine-binding globulin (tbg-chicago). | the five known types of inherited variant t4-binding globulin (tbg) have in common increased sensitivity to heat denaturation compared to the common type tbg (tbg-c). in the course of studies to screen for and characterize variant tbgs in population groups, we detected a unique tbg with marked resistance to heat denaturation. the propositus was a 22-year-old black man without personal or family history of thyroid disease. his native tbg (ntbg) in serum had a t1/2 of denaturation at 56 c of 90 mi ... | 1986 | 3093522 |
| the influence of hemoglobin level on the regression and long term local control of transitional cell carcinoma of the bladder following photon irradiation. | patients with transitional cell carcinoma of the bladder treated by radical megavoltage x ray therapy are analyzed. there was a significant correlation between tumor t stage classification and the hemoglobin level at the start of radical radiotherapy. a hemoglobin level of 12g/dl or more was associated with a significant improvement in complete local tumor regression at 6 months and durable local tumor control for patients with t3 cancer or a cancer of grade 3 histology. hemoglobin level did not ... | 1986 | 3093414 |
| effect of glibenclamide on thyroid hormone metabolism in rats. | this study was undertaken to elucidate the effect of glibenclamide, one of sulfonylurea drugs, on thyroid hormone metabolism in vivo and on the conversion of thyroxine (t4) to 3,5,3'-triiodothyronine (t3) in the isolated perfused rat liver and kidney. glibenclamide (0.2 mg/kg body weight) was intraperitoneally administered to normal and streptozotocin-induced (50 mg/kg) diabetic rats for 14 days. the liver and kidney of normal rats were perfused for 30 minutes with a synthetic medium containing ... | 1986 | 3093349 |
| human thymocyte maturation in vitro: a flow cytometric analysis. | using an in vitro culture system, light scatter analyses, and two-color flow cytometry, we provide evidence that the interleukin-2 (il-2) and transferrin receptors can be induced within 48 hr on nonproliferating immature thymocytes. the thymocytes (greater than 35%) that expressed the transferrin and il-2 receptors demonstrated nuclear activation as measured by log 90 degrees light scatter analysis. increases in antigen-receptor-associated t3-antigen expression followed transferrin and il-2-rece ... | 1986 | 3093098 |
| the role of il-2 and t4+ cells in the generation of human influenza virus-specific ctl activity. | stimulation of human peripheral blood lymphocytes (pbl) with influenza a virus leads to the generation of virus-specific cytotoxic t lymphocyte (ctl) activity as well as natural killer (nk)-like activity. in this study, we show that exogenous il-2 augments the in vitro generation of virus-specific ctl activity, only when added some days after the initiation of the culture. apparently, the endogenously produced il-2 can be a limiting factor in the in vitro generation of ctl activity. the increase ... | 1986 | 3093087 |
| immunoblastic lymphadenopathy, angioimmunoblastic lymphadenopathy, and ibl-like t-cell lymphoma. a spectrum of t-cell neoplasia. | thirty cases of immunologically determined and histologically diagnosed immunoblastic lymphadenopathy (ibl), angioimmunoblastic lymphadenopathy (aild), and ibl-like t-cell lymphoma were clinicopathologically reviewed. clinical manifestations and laboratory findings did not reveal significant differences in these three groups. ibl, aild, and ibl-like t-cell lymphoma showed a spectrum of histologic changes, in which proliferation of pale cells was a critical diagnostic point for the histologic mal ... | 1986 | 3093047 |
| distinction of two cd3-monoclonal antibodies in respect to the effectivity to modulate the induction of igm- and gamma-interferon synthesis. | monoclonal antibodies which specifically bind to epitopes within the t3-antigen complex (cd3 mab) are excellent tools to analyse mechanisms of t-cell activation and of the interactions between t-cells, b-cells, accessory cells and intermediating immunefactors. in the present study we could show, that the cd-3 mabs bma 030 and bma 031 are highly effective in modulating immunereactions, similar to antibodies directed to the t4-antigen (cd4-mab) or the t8-antigen (cd8-mabs) respectively. the data s ... | 1986 | 3092794 |
| a role for t3+4-6-8- transitional thymocytes in the differentiation of mature and functional t cells from human prothymocytes. | in vivo, immunocompetent t lymphocytes are only detected late in ontogeny, among mature thymocytes expressing either t4 (l3t4 in mouse) or t8 (lyt-2) surface glycoproteins. we have previously shown, however, that there are functional precursors among t3+4-6-8- human thymocytes in vivo. here we report on the in vitro differentiation of prothymocytes into t3+4-6-8- and mature t cells. t11+3-4-6-8- prothymocytes (0.5% of total thymocytes, greater than 98% pure) were obtained after treatment of thym ... | 1986 | 3092222 |
| sigma factors from e. coli, b. subtilis, phage sp01, and phage t4 are homologous proteins. | we show, using dot matrix comparisons and statistical analysis of sequence alignments, that seven sequenced sigma factors, e. coli sigma-70 and sigma-32, b. subtilis sigma-43 and sigma-29, phage sp01 gene products 28 and 34, and phage t4 gene product 55, comprise a homologous family of proteins. sigma-70, sigma-32, and sigma-43 each have two copies of a sequence similar to the helix-turn-helix dna binding motif seen in crp, and lambda repressor and cro proteins. b. subtilis sigma-29, sp01 gp28, ... | 1986 | 3092189 |
| a case of total thyroxine-binding globulin deficiency with graves' disease: fluctuations of plasma triiodothyronine/thyroxine ratio. | a 37-year-old male with total thyroxine-binding globulin (tbg) deficiency associated with graves' disease is described. both tbg immunoreactivity and tbg capacity were not detectable in his serum. serum concentrations of thyroxine-binding prealbumin and albumin were normal. he was initially hyperthyroid. during methimazole-treatment he was maintained in an euthyroid state except for two short hypothyroid periods. his plasma triiodothyronine/thyroxine (t3/t4) ratios during both the untreated hype ... | 1986 | 3091890 |
| leu-3/t4 expression on epidermal langerhans cells in normal and diseased skin. | recent evidence exists that the expression of the leu-3/t4 antigen is not restricted to thymus-derived lymphocytes but can also be detected on mononuclear phagocytes and epidermal langerhans cells (lc). when searching for the presence of leu-3/t4 antigen-bearing cells in tissue sections of a variety of inflammatory and neoplastic skin disorders, we observed quantitative and qualitative differences in the intensity of anti-leu-3a labeling of epidermal dendritic cells. reasoning that leu-3/t4 expr ... | 1986 | 3091703 |
| sustained rises in serum thyrotropin, thyroxine, and triiodothyronine during long term, continuous thyrotropin-releasing hormone treatment in patients with amyotrophic lateral sclerosis. | in a pilot therapeutic trial, four patients with amyotrophic lateral sclerosis (als) were treated with long term, continuous infusions of trh, three intrathecally and one epidurally. they had prompt increases in serum tsh and thyroid hormone concentrations, averaging 120% for tsh, 49% for serum t4, 68% for the serum free t4 index, 49% for serum t3, and 67% for the serum free t3 index. these elevations were statistically significant for all but serum t3 and persisted for the duration of treatment ... | 1986 | 3091628 |
| time-dependent effects of oral thyrotropin-releasing hormone in hyperthyroid female rats. | we have studied the thyrotropin (tsh) and thyroid hormone (t3) response to acute thyrotropin-releasing hormone (trh) administration in rats previously rendered hyperthyroid by daily ip injection of 25 micrograms l-t4/100 g bw for 30 days. animals were thereafter put on distilled water or trh (2 mg/10 ml) as drinking solution for another 18 days, continuing t4 administration. on days 3 and 10 on oral trh, the serum tsh and t3 response to trh was studied by administering 2 micrograms of the tripep ... | 1986 | 3091472 |
| effects of in vivo bolus versus continuous trh administration on tsh secretion, biosynthesis, and glycosylation in normal and hypothyroid rats. | the effects of in vivo trh administered either as bolus or continuous doses on tsh secretion, synthesis, and glycosylation were studied in normal and hypothyroid rats. nine-week-old normal or 3-week postthyroidectomy rats were administered bolus doses of saline or trh (0.5 mg/kg) twice daily or continuous saline or trh (1 mg/kg/day) via an osmotic pump. after 5 days, pituitaries were removed and incubated with [35s]methionine (met) and [3h]glucosamine (glcn), with or without 10(-8) m trh, for 6 ... | 1986 | 3091425 |
| cultured human thymus epithelial monolayer cells induce cd4 expression on mononuclear cells of aids patients in vitro. | we investigated the in vitro effect of cultured human thymic epithelial monolayer cells on mononuclear cells (mnc) from patients with acquired immune deficiency syndrome (aids) and aids-related complex (arc). patients having undergone bone-marrow transplantation (bmt), who showed a similar deficiency of t-lymphocytes expressing cd4 (leu-3, t4), and healthy blood donors served as controls. most epithelial monolayer cells were of thymic medulla origin, as documented using a panel of monoclonal ant ... | 1986 | 3091303 |
| an improved approach to thyroid function testing in patients with non-thyroidal illness. | we have compared the results of serum thyrotrophin (tsh) measurements using a sensitive immunoradiometric assay (irma) with those of conventional thyroid function tests in 299 hospital inpatients with a range of non-thyroidal illnesses. levels of total thyroxine (t4), free t4, total tri-iodothyronine (t3) and free t3 in the hypothyroid range were recorded in 8%, 15%, 19% and 49% of patients, respectively, whereas tsh (irma) was abnormally low in 1%. furthermore, basal tsh (irma) accurately predi ... | 1986 | 3091294 |
| different effects of il-2 addition or antibody crosslinking on t-cell subset stimulation by cd3 antibodies. | the effect of exogenous recombinant interleukin-2 (il-2) or of antibody crosslinking on the activation of human t-cell subsets by igg2a (okt3/bma030), igg1 (leu4 and ucht1), or igg2b (bma031) anti-t3 antibodies (cd3) was investigated. in so-called nonresponder cultures as well as in monocyte-depleted cell cultures addition of il-2 increased the cd3-induced activation and proliferation of t4 and t8 cell subsets. relatively more t8 than t4 cells were stimulated by antibody binding and il-2. crossl ... | 1986 | 3091264 |
| serum concentrations of 3,5,3',5'-tetraiodothyroacetate (t4a) in subjects with hypo-, hyper- and euthyroidism. | some factors influencing serum 3,5,3',5'-tetradiodothyroacetate (t4a) concentrations were examined in hypothyroid, euthyroid and hyperthyroid human subjects. serum t4a concentration was shown to be correlated with parameters such as serum, total and free thyroxine (t4) concentrations and thyroxine: thyroxine binding globulin ratio, which are indicative of the availability of t4 for peripheral metabolism. the relationship between t4a and these parameters was not a simple linear one; serum levels ... | 1986 | 3090812 |
| effect of short-term lithium carbonate administration in hyperthyroidism, with or without associated ophthalmopathy. | the study was carried out on 21 female hyperthyroid patients aged 22-53 years, with or without associated ophthalmopathy, admitted to the endocrinology ward of the 1st medical clinic of the craiova county hospital, romania, between 1983 and 1985. administration of lithium carbonate was proceeded and followed by radioimmunoassay (ria) determinations of t3 and t4 and measurement of achilles' reflex (ar). two regimens were employed. the first comprised ten patients receiving doses of 500 mg/day lit ... | 1986 | 3090679 |
| long-term effects of trh administration on food intake and body weight in the rat. | the effect of long-term trh administration through drinking water (0.2 mg/ml) on food and water intake and body weight has been studied in three groups of female rats: (1) thyroidectomized, (2) thyroidectomized receiving daily 250 micrograms/kg of l-t4, (3) sham-operated. treatment with oral trh for 30 days decreased body weight and increased food intake in sham-operated rats. no trh effects on body weight or food consumption were observed in either of the other groups of thyroidectomized rats. ... | 1986 | 3090567 |
| [lactotropic and thyrotropic functions of the hypophysis in polycystic ovary syndrome]. | an increase in the prolactin basal level was detected in 8 out of 50 patients with ovarian polycystosis. a test with trh and a simultaneous determination of the levels of prolactin, tth, t3, t4 were used in 25 patients, of them a hyperergic reaction of prolactin to trh was detected in 5. a correlation analysis of function of the thyroid was performed. positive correlation between the levels of prolactin and tth in patients with hyperprolactinemia and between a maximum increment in percentage to ... | 1986 | 3090541 |
| thyroid hormones and experimental cancer cachexia. | the relationship between circulating thyroid hormones and nutritional status was studied in sarcoma-bearing inbred c57bl/6j mice and control mice. supplementation with exogenous thyroxine (t4) was also evaluated. tumor-bearing animals had depressed levels of circulating thyroid hormones. this was also found in food-restricted (pair-fed and pair-weighed) controls. plasma levels of thyroid hormones decreased with increased tumor burden. thyrotropin-releasing hormone caused an increased response of ... | 1986 | 3090341 |
| in vitro restoration by interleukin-2 (il-2) of the impaired natural killer cell activities, il-2 receptor expression, and t cell proliferation in hemophilia. | five of 22 hemophiliacs who were seropositive for human t cell leukemia virus iii (htlv iii) and manifested severe impairment of immune parameters (both in vivo and in vitro) similar to those observed in patients with clinical symptoms of acquired immune deficiency syndrome (aids) were chosen for this study. profound lymphopenia was observed in four of five patients with decreased and qualitatively impaired helper/inducer (t4) cells and increased t suppressor/cytotoxic (t8) cells. observed in al ... | 1986 | 3090209 |
| hypothyroidism of hypothalamic origin in pyridoxine-deficient rats. | pyridoxine-deficient young rats (3 weeks old) had significantly reduced levels of pituitary tsh, serum thyroxine (t4) and tri-iodothyronine (t3) compared with pyridoxine-supplemented rats. the status of the pituitary-thyroid axis of normal, pyridoxine-supplemented and pyridoxine-deficient rats was evaluated by studying the binding parameters of [3h](3-methyl-histidine2)trh in the pituitary of these rats. the effects of trh and t4 injections on pituitary tsh and serum tsh, t4 and t3 of these two ... | 1986 | 3090182 |
| enumeration of the t4 positive and t8 positive lymphocytes in aids and related syndromes. comparison of the immunogold and the immunofluorescence techniques. | immunofluorescence and immunogold techniques yielded similar results when used to determine the t lymphocyte subsets (t4 and t8 positive cells) in patients with aids or aids-related complex and in healthy homosexual men seropositive for htlv iii/lav antibody. in these pathological situations, the advantages of immunogold staining could render this technique useful in a clinical laboratory. | 1986 | 3090151 |
| biosynthesis and surface expression of t8 by peripheral blood t4+ cells in vitro. | biosynthetic labeling with 35s-methionine and 35s-cysteine of isolated t4+ cells from con a-activated t cells demonstrated that the t8 antigen was synthesized by activated t4+ cells. two-color fluorescence analysis of the activated t cell population from which the t4+ fraction was obtained showed that both t4+t8- and t4+t8+ cells were present. the t8 antigen that was immunoprecipitated by monoclonal anti-t8 from activated t4+ cells migrated with an electrophoretic mobility corresponding to an m. ... | 1986 | 3090142 |
| transient modulation and internalization of t4 antigen induced by phorbol esters. | phorbol esters are known to alter the expression of surface antigens and receptors on a variety of mammalian cell types. on t lymphoblastoid cell lines and peripheral blood t cells, phorbol esters have been shown to selectively reduce the expression of the t4 antigen. to more fully characterize this process, we have examined the metabolic requirements for this phorbol ester effect, and have evaluated the relationship between phorbol ester-induced t4 loss and the expression of receptors for phorb ... | 1986 | 3090141 |
| the role of thyroxine (t4)-binding serum proteins in oleic acid-induced increase in free t4 in nonthyroidal illnesses. | we studied the effects of various concentrations of oleic acid (0.1-3.3 mm), such as are often found in the serum of patients with nonthyroidal illness, on the dialyzable (free) fraction of t4 (dft4) in three types of serum samples: pooled normal serum (pns), pooled pregnancy serum (pps), and pooled serum of hospitalized patients (pshp). the samples were extracted with diethyl ether to remove endogenous fatty acids before being tested in the dft4 assay. oleic acid caused only a moderate increase ... | 1986 | 3090096 |
| goiter size and thyroid function in an endemic goiter area in northern italy. | thyroid function was studied in a large number of subjects residing in varsi, a town in the province of parma, italy. in this area, endemic goiter associated with moderate iodine deficiency [59 +/- 3 (+/- se) microgram iodine/g creatinine], as defined by who criteria, affects 65% of the population. serum t4, t3, thyroglobulin (tg), and tsh concentrations were measured by ria in 1218 subjects. the tsh response to trh was determined in 108 subjects selected randomly from the groups with different ... | 1986 | 3090091 |
| changes in thyroid function tests induced by 2 month carbamazepine treatment in l-thyroxine-substituted hypothyroid children. | in five l-thyroxine-substituted hypothyroid children with partial epilepsy serum total thyroxine (t4) and free t4 (ft4) significantly (p less than 0.01) decreased following 2 months of carbamazepine (cbz) administration (20 mg/kg per bw per day) from mean (+/- sd) values of 12.7 +/- 1.1 micrograms/dl and 15.5 +/- 1.8 pg/ml to mean values of 7.5 +/- 2.3 and 10.1 +/- 1.7, respectively. in all but one patient important changes in both serum total and free triiodothyronine (t3, ft3) were not observe ... | 1986 | 3089800 |
| evaluation of the pituitary-thyroidal axis in newborns undergoing exchange transfusion. | to evaluate whether the hypothyroxinaemia, previously noted in hyperbilirubinaemic newborns immediately after exchange transfusion for rh or ab0 incompatibility, was due to impairment in the secretion of thyroid stimulating hormone (tsh) by the pituitary, we studied the thyroid hormone response to thyrotropin releasing hormone (trh) and compared this response to that seen in a control population of healthy neonates. all infants studied responded with a brisk tsh increase; 30 min after trh inject ... | 1986 | 3089799 |
| t-cell differentiation antigens and antigenic lymphocyte reactivity in pleural effusions. | blood and pleural effusion mononuclear cells from thirteen patients were examined for the expression of t lymphocyte differentiation antigens as well as in vitro thymidine incorporation. the ratio of t4 to t8 cells was significantly greater among pleural effusion lymphocytes than among blood lymphocytes. effusion lymphocyte responses to phytohaemagglutinin were less than those of blood lymphocytes. unstimulated thymidine incorporation was greater in pleural effusion lymphocytes. antigen-stimulat ... | 1986 | 3089237 |
| leu-m1 antigen expression in advanced (tumor) stage mycosis fungoides. | the authors describe two patients with clinically and histopathologically documented advanced (tumor) stage mycosis fungoides. in each case the large, pleomorphic neoplastic cells lacked the monoclonal antibody-defined cell surface antigens commonly associated with immature and mature t-cells, i.e., t11, leu-1, t3, t4, t6, t8, and t10, but expressed various t-cell-associated activation antigens, such as hla-dr, tac, and t21. leu-m1, a monocyte-associated antigen, was not expressed by the small, ... | 1986 | 3088980 |
| [growth in weaned piglets and levels of iodothyronines in the blood during the administration of thyroactive substances]. | in five trials with 100 piglets we studied the effects of thyreoglobulin, potassium nitrite and the thyreostatic preparation carbimazol, which were added to a complete starter in the period from early weaning to the age of four weeks. changes in the concentrations of thyroxine (t4) and triiodothyronine (t3) in the blood serum of control unmedicated piglets weaned from the sows can be characterized, after a short rise, by a decrease which stopped usually in the fourth week, accompanied by higher ... | 1986 | 3088799 |
| value of radiation therapy in addition to surgery for cancer of the head and neck. | this historically controlled study evaluates radiation therapy in 119 patients--with squamous cell carcinomas of the head and neck--who underwent surgery alone (sa) or surgery plus radiation (s + r). the primary tumor control and nodal control, in patients with negative surgical margins who had surgery alone (sa), were 63% in the oral cavity, 60% in the oropharynx, and 67% in the hypopharynx. the same rates for s + r group and negative surgical margins were 100%, 73%, and 100%. combining the pat ... | 1986 | 3088523 |
| [determination of lymphocyte subpopulations in multiple myeloma and monoclonal gammopathies of undetermined significance]. | by means of monoclonal antibodies, the lymphocyte subpopulation in 12 patients affected by multiple myeloma, 3 by waldenström's macroglobulinemia, 7 by m.g.u.s. and 8 patients affected by accompanying monoclonal gammopathy have been determined. the group of patients affected by multiple myeloma or waldenström's macroglobulinemia presented, against control, a significant reduction in the ok t3+ and ok t4+ cell percentage, with a remarkable reduction of the ok t4+/ok t8+ ratio. the ok t8+ cell ave ... | 1986 | 3088491 |
| serum and peritoneal dialysate thyroid hormone levels in patients on continuous ambulatory peritoneal dialysis. | thyroid function tests were performed on 16 clinically euthyroid patients with end-stage renal failure undergoing regular haemodialysis or continuous ambulatory peritoneal dialysis and compared with 8 healthy subjects. the patient groups were carefully matched, especially regarding relative duration of dialysis (mean of 24 months). total serum thyroxine, total triiodothyronine, free thyroxine, free triiodothyronine and reverse triiodothyronine were significantly lower in both patient groups than ... | 1986 | 3088462 |
| isolation of pure functionally active cd8+ t cells. positive selection with monoclonal antibodies directly conjugated to monosized magnetic microspheres. | a monoclonal antibody of the igm isotype, iti-5c2, which binds with high affinity to cd8 molecules, was directly conjugated to the monosized magnetic microspheres m-450. this permits selective removal of the cd8+ t cell subset (t8) from peripheral blood mononuclear cell suspensions in a rapid one-step procedure. with a low ratio of microspheres to cells (2:1), functionally active t8 cells can be recovered. in vitro experiments involving such positively selected t8 cells or recombinations of isol ... | 1986 | 3088118 |
| t cell replacing factor for steroids (trf-s): a 40,000 dalton protein produced by a t4+ t cell. | the induction of polyclonal immunoglobulin (ig) synthesis by glucocorticosteroids (gcs) in human peripheral blood lymphocytes is dependent on both t cells and monocytes. t cells can be replaced by a cytokine, t cell replacing factor for steroids (trf-s), which promotes gcs-induced ig production. t cells produce the cytokine when cultured with intact monocytes, with 24 hr monocyte supernatants, or with small quantities (0.1 u/ml or more) of highly purified interleukin 1 (il 1). trf-s was produced ... | 1986 | 3088104 |
| thyrotropin secretion in thyrotoxic and thyroxine-treated patients: assessment by a sensitive immunoenzymometric assay. | a new tsh immunoenzymometric assay was found to be capable of discriminating between the serum tsh values of normal subjects [2.28 +/- 1.02 (+/-sd); range, 0.6-6.5 microu/ml] and those of clinically euthyroid, antithyroid drug-treated (n = 22) or clinically thyrotoxic (n = 34) patients. while a wide spectrum of basal tsh values was found in the antithyroid drug group [ranging from undetectable (less than 0.05 microu/ml: 57%) to 17.9 microu/ml], all clinically thyrotoxic patients had undetectable ... | 1986 | 3088021 |
| the effect of environmental temperature on concentrations of thyroxine and triiodothyronine after thyrotropin releasing hormone in steers. | twenty-four angus x hereford steers (155 +/- 4 kg) were used to examine thyroid function during exposure to ambient temperatures of 4, 18 and 32 c. jugular cannulae were inserted after steers were acclimated to individual stalls in environmentally controlled chambers at 18 c for 3 d. the day following cannulation, ambient temperatures were changed 2 c/h for 7 h and serum samples were collected hourly. after steers were exposed to either 4, 18 or 32 c for 1 and 72 h, thyrotropin releasing hormone ... | 1986 | 3087927 |
| circulating thyroid hormones in primates with mild or severe hepatitis following liver transplantation. | in order to study the effects of acute immunologically mediated liver disease on circulating thyroid hormones, serum levels of thyroxine (t4, total and free) and triiodothyronine (t3) were measured in 8 baboons before and for 60 days after allogeneic liver transplantation. in 3 animals early rejection and jaundice developed; t4 levels declined as liver function deteriorated. in the 5 tolerant animals liver function was only temporarily deranged without jaundice and there was a consistent early r ... | 1986 | 3087852 |
| thyroxine-binding globulin in spontaneous abortion. | thyroxine-binding globulin (tbg), thyroxine-binding prealbumin (tbpa), thyroxine (t4), free t4, triiodothyronine (t3) and thyroid-stimulating hormone (tsh) were studied in 144 apparently healthy, euthyroid pregnant women displaying miscarriages. in an additional group of 18 aborting women a frozen blood sample drawn in early pregnancy was similarly analyzed for the thyroid components as well as for human chorionic gonadotropin (hcg). the values were compared to those of normal pregnancies (n = 2 ... | 1986 | 3087828 |
| iodide-induced hypothyroidism in a patient with anorexia nervosa. | a 39-year-old woman who had been suffering from anorexia nervosa was found to have hypothyroidism. serum t4, free t4, t3, free t3 and tsh were 3.19 micrograms/dl, 0.5 ng/dl, 15.3 ng/dl, 1.2 pg/ml and 162.1 microu/ml, respectively. on careful questioning, she was found to have taken an iodine-rich diet. the serum iodine concentration was 122 micrograms/dl (normal: 4-9 micrograms/dl) and urinary iodide excretion was 13.05 mg/day (normal: less than 2 mg). after withdrawal of the iodine-rich diet, h ... | 1986 | 3087739 |
| low t3 syndrome in cancer patients in relation to weight loss, intravenous hyperalimentation therapy and age. | in order to investigate the relation of weight loss and intravenous hyperalimentation therapy to low t3 syndrome, serum t3, t4. rt3 and tbg were determined by radioimmunoassay in 105 cancer patients. the cancer patients were classified into 3 groups, group i, ii and iii depending on the grade of weight loss, ranging up to a 5% change in weight loss from a healthy condition, from 5 to 9%, and more than 10%, respectively. cancer patients under age 59 showed no significant difference in serum t3, t ... | 1986 | 3087738 |
| discordant effects of thyrotropin (tsh)-releasing hormone on pre- and posttranslational regulation of tsh biosynthesis in rat pituitary. | to investigate whether trh regulates tsh production through a pre- or posttranslational mechanism, we determined the pituitary levels of mrnas for alpha-subunit and tsh beta in male sprague-dawley rats given trh in the presence or absence of thyroid hormones, with or without hypothalamic influence. in normal rats, serum tsh increased 6-fold after a single sc injection of trh (7 micrograms/kg bw), but the levels of mrna for both tsh subunits did not differ from the control values. infusion of trh ... | 1986 | 3087736 |
| t4: t8-lymphocyte ratios in human bone marrow: the effect of sampling technique. | marrow aspirate and trephine biopsy t4 : t8-lymphocyte ratios were compared with peripheral blood values. to do this, a new technique for harvesting marrow cells from trephine biopsies is described. results show that blood and trephine ratios did not overlap. marrow aspirate ratios were intermediate suggesting that peripheral blood contamination was causing aspirate t4 : t8 ratios to be falsely elevated. | 1986 | 3087686 |
| postnatal development of brain trh, serum tsh and thyroid hormones in the male and female rat. | the postnatal development of immunoreactive trh in the central nervous system (cns), serum tsh and thyroid hormones was studied in both male and female normal rats. while in most structures of the cns, trh increased until day 20-30, serum tsh values peaked at day 15 as did t4. significant differences were also obtained between both sexes in these parameters. these data further support the fact that pituitary-thyroid axis maturation is independent of brain trh. | 1986 | 3087133 |
| [unwanted modification of the thyroid gland by drugs with special reference to nonsteroidal antirheumatic agents]. | in two retrospective clinical studies was investigated the influence of the modern non-steroidal antirheumatic drugs indometacin and diclofenac (rewodina) on the thyroid gland and corresponding peripheral hormone parameters. under longterm treatment with indometacin a moderate strumigenic effect could be observed, which could not clearly be proved under the diclofenac therapy. in all patients with rheumatoid arthritis, independent of the kind of pharmacotherapy, decreased t3-hormone levels were ... | 1986 | 3087075 |
| failure of carbon disulfide and levothyroxine to modify the cardiovascular response of rabbits to a high-cholesterol diet. | exposure of rabbits for 12 weeks to 300 ppm carbon disulfide (cs2) for 6 h/day, 5 days/week, or to 25 mg/day of thiourea or 2% cholesterol in the diet, or to any combination thereof caused a significant reduction in the concentration of serum thyroxine (t4). the reduction of the concentration of serum t4 in rabbits by the treatments was completely offset by the inclusion of 0.1 mg/day of sodium levothyroxine in the diet. ingestion of feed containing 2% cholesterol significantly increased the deg ... | 1986 | 3087026 |
| partial primary structure of the t4 antigens of mouse and sheep: assignment of intrachain disulfide bonds. | the t4 antigens of mouse and sheep have been purified to homogeneity and partially sequenced using protein and peptide microsequencing techniques. both mouse and sheep antigens bear distinct homology to human t4, having an amino-terminal segment that is homologous to the k-chain variable region (vk). a surprisingly high degree of sequence divergence was nevertheless evident between the t4 antigens of the three mammalian species, suggesting an unusually rapid rate of evolution that is possibly re ... | 1986 | 3086886 |
| the gene encoding the t-cell surface protein t4 is located on human chromosome 12. | the surface glycoproteins t4 and t8 define functionally distinct populations of t lymphocytes. we have obtained cdna and genomic clones encoding the t4 molecule and used these as probes to determine the chromosomal location of this gene. genomic blotting experiments, along with in situ hybridization analyses, indicate that the t4 gene resides on the short arm of human chromosome 12, at region p12-pter. thus, the t4 gene is not linked to any known member of the immunoglobulin gene family, includi ... | 1986 | 3086883 |
| do immature t cells accumulate in advanced age? | the hypothesis that decreased t cell function in the elderly involves an increased number of less differentiated t cells was examined. three markers known to change during thymocyte development were analyzed; ratio of adenosine deaminase (ada) to purine nucleoside phosphorylase (pnp), lactate dehydrogenase (ld) h/m subunit ratios and the t cell associated antigens, t3, t4, t8 and t10. cells tested were from 10 old (greater than 75 years) and 10 young (less than 35 years) persons with equal numbe ... | 1986 | 3086635 |
| an immunocytochemical and electron microscopic study of the hyt mouse anterior pituitary gland. | the hyt mutant mouse used in this study has a hypoplastic thyroid gland and is characterized by retarded somatic growth, very low to undetectable levels of plasma thyroxine (t4), and increased levels of plasma thyroid-stimulating hormone (tsh). this congenital hypothyroid mouse is therefore an ideal model for studying the effects of thyroid hypofunction on the adenohypophysis. the anterior pituitary of the hyt mouse appeared less granular than that of the normal control when viewed by light micr ... | 1986 | 3086477 |
| administration of monoclonal anti-t cell antibodies retards murine lupus in bxsb mice. | murine lupus in bxsb mice is associated with b cell hyperactivity, monocyte proliferation, and impaired t cell function. however, the significance of these abnormalities, and the relationship among them, has not been clearly established. to examine the role of t cells in the pathogenesis of autoimmune disease in bxsb mice, we depleted specific t cell subsets from bxsb males by using rat igg2b monoclonal antibodies (mab) to either thy-1.2 (on all t cells) or l3t4 (on "helper/inducer" t cells). a ... | 1986 | 3086436 |
| a double-label flow cytometric analysis of the simultaneous expression of okt4 and leu2a antigens on circulating t lymphocytes in myasthenia gravis. | a two-color immunofluorescence analysis of circulating abnormal t lymphocytes bearing both helper (t4) and suppressor/cytotoxic (leu2a) t cell markers (double marker cells) was performed by means of the laser flow cytometry system. the double marker cell level was very low in 6 patients with multiple sclerosis and 12 normal controls studied. fifteen of 31 patients with myasthenia gravis (mg) showed an elevation of double marker cells without an increase in t6-positive cells. the values were sign ... | 1986 | 3086380 |
| progressive change in lymphocyte distribution and degree of hypergammaglobulinemia with age in children with hemophilia. | fifty asymptomatic, pediatric hemophiliacs were examined for distribution of t-cell subsets, responsiveness to mitogen stimulation, interleukin-2 production, hypergammaglobulinemia, and the presence of antibody to virus including the human t-lymphotrophic virus type iii (htlv-iii). hemophilia a patients receiving factor viii concentrate as replacement therapy had the most pronounced changes including decreased t4/t8 ratios and lower in vitro responsiveness to both phytohemagglutinin and pokeweed ... | 1986 | 3086364 |
| isoelectric focusing variant thyroxine-binding globulin in american blacks: increased heat lability and reduced serum concentration. | three major bands of t4-binding globulin (tbg) at ph 4.3-4.5 are seen after isoelectric focusing (ief) of serum. daiger et al. detected a cathodal shift of all ief bands in some american blacks, and found that the inheritance of this variant tbg (tbg-s) is x-chromosome linked. however, the properties of the tbg-s molecule and its possible association with changes in thyroid function have not been studied. based on ief analysis of 114 serum samples from adult american blacks, the gene frequency o ... | 1986 | 3086361 |
| serum concentrations of prolactin, thyroxine and triiodothyronine relative to season and the estrous cycle in the mare. | studies were conducted to characterize circulating concentrations of prolactin, thyroxine (t4) and triiodothyronine (t3) relative to season and the estrous cycle, and to evaluate the ability of thyrotropin-releasing hormone (trh) to induce prolactin secretion in the horse mare. the increase in serum prolactin following the iv injection of 0, 5, 50 and 500 micrograms trh was dose-related, while all three doses of trh induced a significant and comparable increase in serum t4. seasonal variations i ... | 1986 | 3086267 |
| separation, phenotyping and limiting dilution analysis of t-lymphocytes infiltrating human solid tumors. | tumor-infiltrating lymphocytes (til) were obtained by a combination of mechanical release and enzymatic disaggregation from 35 human solid tumors. the number of lymphocytes in til-enriched suspensions varied from 1 x 10(4) to 7.6 x 10(6) per wet gram of tumor. the til preparations separated by differential centrifugation on ficoll-hypaque gradients contained 10-95% of t11+ cells (mean 50%), and tumor cells accounted for the other major cellular component. macrophages, nk cells, b cells and granu ... | 1986 | 3086239 |
| inappropriate thyrotrophin secretion, increased dopaminergic tone and preservation of the diurnal rhythm in serum tsh. | a patient presented with mild hyperthyroidism, elevated serum t4 and t3, and an inappropriately raised serum thyrotrophin (tsh). there was no evidence of pituitary tumour (alpha-subunit secretion and ct scan of the pituitary were normal). the tsh response to trh was greater than normal. the elevated tsh was suppressed by oral triiodothyronine (100 micrograms daily for 10 d). the normal diurnal variation of tsh was preserved. intravenous injection of the dopamine receptor blocking agent domperido ... | 1986 | 3085996 |
| skin allograft rejection by l3/t4+ and lyt-2+ t cell subsets. | the l3/t4+ and lyt-2+ t-cell subsets can be depleted from mice, using selected monoclonal antibodies in vivo, at different times during rejection of, or priming to, allogeneic skin grafts. although l3/t4+ cells are sufficient to reject skin grafts in naive lyt-2-depleted mice, we show that lyt-2+ cells can become involved, after an initial delay, in intact mice. furthermore, these lyt-2+ cells are primed to dominate the accelerated rejection of a normal secondary response. mice depleted of l3/t4 ... | 1986 | 3085299 |
| effect of thyroid hormones on growth hormone secretion in broiler chickens. | plasma concentrations of growth hormone (gh) were observed to be significantly elevated following the administration of thyrotropin-releasing hormone (trh) in young (4- to 12-week-old) but not in adult (20-week-old) broiler chickens. however, adult sex-linked (dw) dwarf hens did respond to trh. treatment with triiodothyronine (t3) but not thyroxine (t4) (at 1 ppm in the diet from hatch) consistently and significantly reduced the growth rate and decreased the plasma concentrations of gh following ... | 1986 | 3085079 |
| involvement of developing sympathetic nervous system in thyroxine-mediated submandibular gland nerve growth factor and epidermal growth factor responses. | thyroxine (t4) administration in mice during the 2nd wk of postnatal life elicits a precocious increase in submandibular gland-nerve growth factor (smg-ngf) and epidermal growth factor (smg-egf) levels, but the mechanism(s) of t4 action has not been studied. the present report examines the role of the developing sympathetic nervous system (sns) in the smg-ngf and egf responses to t4. for this purpose newborn mice were injected with t4 and/or 6-hydroxydopamine, a toxic congener of norephinephrine ... | 1986 | 3085059 |
| human t lymphocytes and monocytes bear the same leu-3(t4) antigen. | an analysis of the cellular distribution, biosynthesis, and structure of the human t lymphocyte antigen leu-3(t4) was performed. by using a sensitive elisa as well as facs analysis, relative quantities of the leu-3(t4) antigen from whole cell lysates and from cell surfaces of six cell lines were determined. the t-t hybrid cell line 255.88, and the monocyte/macrophage cell line u937, proved to be high producers of the antigen and were chosen for additional investigation. the leu-3(t4) antigens fr ... | 1986 | 3084644 |
| differential ability of fixed antigen-presenting cells to stimulate nominal antigen-reactive and alloreactive t4 lymphocytes. | the capacity of paraformaldehyde-fixed human antigen-presenting cells (apc) to induce responses by autologous, freshly isolated peripheral blood t4 cells was examined and was compared with their ability to stimulate allogeneic t4 cell dna synthesis. fixation of glass-adherent cells (ac) with as little as 0.06% paraformaldehyde abolished leucine incorporation, whereas fixation with 0.75% paraformaldehyde caused death of greater than 98% of the ac. control apc were able to take up and present the ... | 1986 | 3084635 |
| mechanisms of lymphocyte adhesion to human vascular endothelial cells in culture. t lymphocyte adhesion to endothelial cells through endothelial hla-dr antigens induced by gamma interferon. | the effects of interferons (ifns) on lymphocyte adhesion to cultured human vascular endothelial cells (ec) were investigated using an in vitro assay. endothelial cells obtained from umbilical vein were first cultured at a low density with a conditioned medium (cm) from 12-o-tetra decanoylphorbol 13-acetate-concanavalin a (tpa-con a) stimulated human peripheral blood lymphocytes (pbl), or with recombinant (r) gamma interferon (ifn-gamma) or r alpha interferon (ifn-alpha), and then were incubated ... | 1986 | 3084562 |
| sex hormone-binding protein in hyperthyroxinemic patients: a discriminator for thyroid status in thyroid hormone resistance and familial dysalbuminemic hyperthyroxinemia. | sex hormone-binding globulin (shbg) levels in serum are affected by thyroid status; hyperthyroidism is associated with high shbg levels, whereas hypothyroid patients have low or normal shbg levels. this study was undertaken to test the usefulness of shbg determinations to define the thyroid status in two hyperthyroxinemic states: thyroid hormone resistance (thr) and familial dysalbuminemic hyperthyroxinemia (fdh). serum shbg levels were determined in 193 patients and 26 normal subjects using an ... | 1986 | 3084540 |
| plasmapheresis: an adjunct to medical management of severe hyperthyroidism. | a woman with hyperthyroidism and myasthenia gravis developed respiratory failure in association with radiation-induced thyroiditis. treatment with steroids, propylthiouracil, propranolol, iodine, and plasmapheresis was associated with dramatic reduction in serum triiodothyronine (t3), serum thyroxine (t4), and thyroglobulin levels and prompt recovery of the patient. the medications that this patient received have been shown to cause an abrupt decline in serum t3 levels with little or no effect o ... | 1986 | 3084458 |