Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| proteolytic degradation of connectin, a high molecular weight myofibrillar protein, during heating of meat. | when homogenised muscle (ph 5·5) was heated at 55°c, connectin was extensively degraded, whereas actin and myosin heavy chains were apparently unaffected. it was concluded that carboxyl proteases (e.g. cathepsin d) were largely responsible, because the breakdown of connectin was inhibited by the addition of pepstatin. when whole muscle samples were heated at 50-70°c, degradation of connectin was inversely related to the ultimate ph of the source muscle, again indicating the role of carboxyl prot ... | 1981 | 22054492 |
| effect of electrical stunning method and cardiac arrest on bleeding efficiency, residual blood and blood splash in lambs. | five hundred and fifty-nine southdown-romney cross lambs were used in a comparison of the efficiency of bleeding animals slaughtered by one of three different methods. the main comparison was between spear stuck lambs that had been electrically stunned by either head application or a head-back method. in addition, some unstunned lambs were slaughtered by the traditional new zealand gash cutting method, no longer used by industry but known to produce well bled carcasses. there was no difference i ... | 1981 | 22054488 |
| residual blood in lamb muscles. | the effect of slaughter method on the residual blood content of the m. longissimus dorsi, the microbiological status of the meat and its tenderness was examined with lambs. there were no differences in any of the characteristics examined between the various stunning procedures used, even though there were marked differences in the amount of blood collected after slaughter. | 1981 | 22054487 |
| studies on the meat production characteristics of botswana goats and sheep-part i: sampling, methods and materials, and measurements on the live animals. | a carcase evaluation exercise was carried out on various groups of smallstock (goats and sheep) which are of importance to the meat industry in botswana, and which formed part of a larger overall project designed to improve meat production from indigenous smallstock. the main objective was to provide background data on various body and carcase characteristics, from which subsequent selection, breeding and improved management could be evaluated and monitored. the data collected was concerned with ... | 1977 | 22054429 |
| escherichia coli o157:h7 populations in ruminants can be reduced by orange peel product feeding. | foodborne pathogenic bacteria such as escherichia coli o157:h7 are threats to the safety of beef. citrus peel and dried orange pulp are by-products from citrus juice production that have natural antimicrobial effects and are often incorporated into least-cost ration formulations for beef and dairy cattle. this study was designed to determine if orange peel and pulp affected e. coli o157:h7 populations in vivo. sheep (n = 24) were fed a cracked corn grain-based diet that was supplemented with a ... | 2011 | 22054194 |
| osseointegration of porous titanium implants with and without electrochemically deposited dcpd coating in an ovine model. | abstract: | 2011 | 22053991 |
| the chemokine receptor cxcr4 and its ligand cxcl12 are activated during implantation and placentation in sheep. | the progression of implantation and placentation in ruminants is complex and is regulated by interplay between sex steroids and local signaling molecules, many of which have immune function. chemokines and their receptors are pivotal factors in implantation and vascularization of the placenta. based on known critical roles for chemokine receptor 4 (cxcr4) during early pregnancy in other species, we hypothesized that cxcr4 and its ligand cxcl12 would increase in the endometrium and conceptus in r ... | 2011 | 22053725 |
| Assessment of right ventricular afterload by pressure waveform analysis in acute pulmonary hypertension. | To characterize hydraulic right ventricle (RV) afterload by pulmonary arterial pressure waveform analysis in an acute pulmonary hypertension (PH) model. | 2011 | 22053220 |
| the interaction between photoperiod and nutrition and its effects on seasonal rhythms of reproduction in the ewe. | in sheep, the seasonal patterns of reproductive activity are driven primarily by the annual photoperiodic cycle but can also respond to other environmental factors such as nutrition, yet little is known about the mechanisms underlying this interaction. this study was designed to define the interaction between photoperiodic and nutritional cues on seasonal patterns of ovarian activity, and to determine if there is a central interaction between these cues. groups of ile-de-france ewes were maintai ... | 2011 | 22053094 |
| inflammation in utero exacerbates ventilation-induced brain injury in preterm lambs. | background: cerebral blood flow disturbance is a major contributor to brain injury in the preterm infant. the initiation of ventilation may be a critical time for cerebral haemodynamic disturbance leading to brain injury in preterm infants, particularly if they are exposed to inflammation in utero. we aimed to determine whether exposure to inflammation in utero alters cardiopulmonary haemodynamics resulting in cerebral hemodynamic disturbance and related brain injury during the initiation of ven ... | 2011 | 22052871 |
| time to generate ventilator-induced lung injury among mammals with healthy lungs: a unifying hypothesis. | to investigate ventilator-induced lung injury (vili), several experimental models were designed including different mammalian species and ventilator settings, leading to a large variability in the observed time-course and injury severity. we hypothesized that the time-course of vili may be fully explained from a single perspective when considering the insult actually applied, i.e. lung stress and strain. | 2011 | 22052185 |
| [virulence factors in proteus spp. bacteria isolated from urinary tract infections: their detection and importance]. | nosocomial infections associated with biofilm formation have been a serious problem in recent years. up to 32 % of them are urinary tract infections in patients with long-dwelling catheters. catheters represent an ideal surface for bacterial adhesion, facilitating easier colonization of the urinary tract. important pathogens causing these infections are bacteria of the genus proteus that colonize catheters not only by biofilm formation but also using other virulence factors. those were developed ... | 2011 | 22052099 |
| myocardial performance and its acute response to angiotensin ii infusion in fetal sheep adapted to chronic anemia. | fetal chronic anemia causes lengthening of cardiomyocytes. in adults, severe left ventricular overload may lead to irreversible ventricular dysfunction. we hypothesized that in sheep fetuses with chronic anemia, remodeled myocardium would less successfully respond to angiotensin ii (at ii) infusion than in fetuses without anemia. a total of 14 ewes with twin pregnancy underwent surgery at 113 ± 1 days of gestation. after a recovery period, anemia was induced by isovolumic hemorrhage in 1 fetus o ... | 2011 | 22051849 |
| Multilocus phylogeographical analysis of Trypanosoma (Megatrypanum) genotypes from sympatric cattle and water buffalo populations supports evolutionary host constraint and close phylogenetic relationships with genotypes found in other ruminants. | Species of the subgenus Trypanosoma (Megatrypanum) have been reported in cattle and other domestic and wild ruminants worldwide. A previous study in Brazil found at least four genotypes infecting cattle (Bos taurus), but only one in water buffalo (Bubalus bubalis). However, the small number of isolates examined from buffalo, all inhabiting nearby areas, has precluded evaluation of their diversity, host associations and geographical structure. To address these questions, we evaluated the genetic ... | 2011 | 22051399 |
| clinical ocular findings in cows with malignant catarrhal fever: ocular disease progression and outcome in 25 cases (2007-2010). | objective to describe the ocular clinical signs in a group of cows diagnosed with sheep associated malignant catarrhal fever (mcf), and to investigate a possible correlation between the severity and progression of certain typical clinical signs and disease outcome. procedure this prospective study involved cows diagnosed with mcf between 2007 and 2010, and included cows enrolled in a larger clinical trial. signalment of affected cows, presence and progression of ocular lesions, as well as dise ... | 2011 | 22051372 |
| characterization and differentiation of equine tendon-derived progenitor cells. | mesenchymal stem cells have been recently investigated for their potential use in regenerative medicine. population of adult stem cells were recently identified in human and lab animal tendons, but no detailed investigations have been made in the equine species. the aim of our study is to identify a progenitor cell population from tendon tissue (tspcs) in the horse superficial digital flexor tendon that are able to be highly clonogenic, to grow fast and to differentiate in different induced cell ... | 2011 | 22051173 |
| evaluation of chondrocyte behavior in a new equine collagen scaffold useful for cartilage repair. | association of biomaterials with autologous cells can provide a new generation of implantable devices for cartilage repair. an ideal scaffold should possess a preformed three-dimensional shape, fix the cells to the damaged area and prevent their migration into the articular cavity. furthermore, the constructs should have sufficient mechanical strength to facilitate handling in a clinical setting and stimulate the uniform spreading of cells and a phenotype re-differentiation process. the aim of t ... | 2011 | 22051171 |
| Comparison of loop-mediated isothermal amplification (LAMP) and real-time PCR method targeting a 529-bp repeat element for diagnosis of toxoplasmosis. | Loop-mediated isothermal amplification (LAMP) is a simple method that can amplify DNA with high specificity, sensitivity, and rapidity. In this study, we compared the performance of LAMP and real-time PCR assays for diagnosis of toxoplasmosis. We designed a real-time PCR assay targeting a 529bp element repeated 200-300 times in the Toxoplasma gondii genome. The detection limits of the LAMP and real-time PCR assays were 10fg/µL and 1fg/µL of T. gondii DNA, respectively. Conventional PCR, LAMP, an ... | 2011 | 22051073 |
| observations on the ultrastructure of equatorial scleral collagen fibrils in sheep eyes. | objective to investigate collagen fibrils of the equatorial sclera in relation to the age-related changes in eye size in sheep. animals studied lambs and outbred ewes. procedures sheep eyes (three lamb and three from adult outbred ewes), presumed disease-free, were processed for transmission electron microscopy (tem) immediately postmortem. tissue blocks from the equatorial region were sectioned across fibril bundles orientated along the equator. micrographs including at least 500 fibrils wer ... | 2011 | 22050829 |
| [shrnas driven by k14 promoter induce tissue-specific rna interference]. | rna interference is an efficient method for exploring gene function. accumulating evidence suggests that rna pol ii promoters can direct cell- or tissue-specific gene silencing. a egfp-shrna fusion construct transcribed from an rna pol ii promoter (k14 promoter) was used to induce gene-specific shrna silencing ofbmp4 gene expression. recombinant vectors (pegfp-c1-shrna, psicheck-bmp4, and pegfp-k14-shrna) were constructed. vectors pegfp-c1-shrna and psicheck-bmp4 were cotransfected into hela cel ... | 2011 | 22049690 |
| [the gene ontology and electro localization of ovine skin derived est-ssr markers]. | abstract: in order to study the potential gene function of ovine est-ssr markers, nine original est of ovine skin derived polymorphic est-ssr loci, which were developed in an early study by our lab, were ontology annotated and electro localized. the results revealed that the original ests of the six loci had high homology with known genes and three of them probably played an important role in wool traits. compared with its cdna library, 8 loci were located on chromosomes of cattle. the homology ... | 2011 | 22049686 |
| Intestinal adenocarcinomas in three generations of sheep. | 2011 | 22049063 | |
| evaluation of immunomodulatory effect of ranahamsa rasayanaya - a sri lankan classical rasayana drug, on experimental animals. | immunity plays a key role in maintaining the health of an individual. therefore, the rational modulation of the immunity through psycho-neuro-endocrine-immune (pni) axis is useful for the prevention as well as for the curing of the diseases. as immunomodulation is a parameter for evaluation of the rasayana effect of a drug, the same has been studied to assess the rasayana effect of ranahamsa rasayanaya (rr). experimental models such as antibody formation against sheep red blood cells (srbc) and ... | 2010 | 22048546 |
| bioengineered self-seeding heart valves. | mechanical and biological prostheses are used to replace damaged heart valves but are associated with significant morbidities. although there is increased interest in bioengineering cell-seeded heart valve scaffolds, it is a time-consuming and technically difficult process. the goal of this project was to engineer self-seeding heart valves that mature quickly in vivo and have a shorter preparation time. | 2012 | 22047685 |
| Bacteriophage lysis of enterococcus host strains: a tool for microbial source tracking? | This paper describes the isolation of Enterococcus host strains, for potential use as simple bacteriophage (phage)-based microbial source tracking (MST) tools. Presumptive Enterococcus host strains were isolated from cattle feces, raw municipal wastewater, agricultural runoff, and waters impacted by farms or wastewater treatment works (WWTW) in southern England, United Kingdom (UK). All enterococcal host strains (n = 390) were first screened for their ability to detect phage in samples of ra ... | 2011 | 22047499 |
| Developing a sustainable phytomanagement strategy for excessive selenium in western United States and India. | Phytomanagement technology is recognized as an inexpensive and environmental friendly strategy for managing natural-occurring selenium (Se) in soils and in poor quality waters. Multi-year field and greenhouse studies were conducted with different plant species in California, USA and Punjab, India under high Se growing conditions. Some of the plant species included; canola (Brassica napus), mustard (B. juncea), broccoli (B. oleracea), spearmint (Mentha viridis), sugarcane (Saccharum officcinarum) ... | 2011 | 22046761 |
| evolutionary history of the vertebrate mitogen activated protein kinases family. | the mitogen activated protein kinases (mapk) family pathway is implicated in diverse cellular processes and pathways essential to most organisms. its evolution is conserved throughout the eukaryotic kingdoms. however, the detailed evolutionary history of the vertebrate mapk family is largely unclear. | 2011 | 22046431 |
| Characterization of protection afforded by a bivalent virus-like particle vaccine against bluetongue virus serotypes 1 and 4 in sheep. | Bluetongue virus (BTV) is an economically important, arthropod borne, emerging pathogen in Europe, causing disease mainly in sheep and cattle. Routine vaccination for bluetongue would require the ability to distinguish between vaccinated and infected individuals (DIVA). Current vaccines are effective but are not DIVA. Virus-like particles (VLPs) are highly immunogenic structural mimics of virus particles, that only contain a subset of the proteins present in a natural infection. VLPs therefore o ... | 2011 | 22046324 |
| an overview of animal prion diseases. | prion diseases are transmissible neurodegenerative conditions affecting human and a wide range of animal species. the pathogenesis of prion diseases is associated with the accumulation of aggregates of misfolded conformers of host-encoded cellular prion protein (prpc). animal prion diseases include scrapie of sheep and goats, bovine spongiform encephalopathy (bse) or mad cow disease, transmissible mink encephalopathy, feline spongiform encephalopathy, exotic ungulate spongiform encephalopathy, c ... | 2011 | 22044871 |
| search for variation in the ovine kap7-1 and kap8-1 genes using polymerase chain reaction-single-stranded conformational polymorphism screening. | keratins and keratin-associated proteins (kaps) are large heterogeneous groups of proteins that constitute about 90% of the wool fiber. the genes encoding the high glycine-tyrosine (hgt) kaps are the first sub-group of kap genes expressed in the wool follicle and just after expression of the keratin genes. little is known about variation in these genes, which led us to investigate two hgt-kap genes, krtap7-1 and krtap8-1. polymerase chain reaction-single-stranded conformational polymorphism anal ... | 2011 | 22044071 |
| Serological monitoring of protection of sheep against Echinococcus granulosus induced by the EG95 vaccine. | Although immunity to Echinococcus granulosus in sheep has been shown to be antibody-mediated and complement-dependent and can be passively transferred in colostrum, in animals vaccinated with EG95, the relationship between protection against an oral challenge infection with E. granulosus eggs and anti-EG95 IgG ELISA absorbance values at the time of challenge has not been satisfactorily proven. Using a combination of results from three EG95 vaccination trials, we have found that the IgG ELISA abs ... | 2012 | 22043983 |
| transcriptional modulation in a leukocyte-depleted splenic cell population during prion disease. | prion replication in the periphery precedes neuroinvasion in many experimental rodent scrapie models, and in natural sheep scrapie and chronic wasting disease (cwd) in cervids. prions propagate in the germinal centers of secondary lymphoid organs and are strongly associated with follicular dendritic cells (fdc) and possibly circulating dendritic cells and macrophages. given the importance of lymphoid organs in prion disease transmission and pathogenesis, gene expression studies may reveal host f ... | 2011 | 22043911 |
| efficient production and cellular characterization of sheep androgenetic embryos. | the production of androgenetic embryos in large animals is a complex procedure. androgenetic embryos have been produced so far only in cattle and sheep using pronuclear transfer (pt) between zygotes derived from in vitro fertilization (ivf) of previously enucleated oocytes. pt is required due to the poor developmental potential of androgenotes derived from ivf of enucleated oocytes. here we compare the developemt to blastocyst of androgenetic embryos produced by the standard pronuclear transfer ... | 2011 | 22043807 |
| multiple pyogranulomas in a katahdin ewe. | 2011 | 22043082 | |
| pregnancy upregulates large-conductance ca(2+)-activated k(+) channel activity and attenuates myogenic tone in uterine arteries. | uterine vascular tone significantly decreases whereas uterine blood flow dramatically increases during pregnancy. however, the complete molecular mechanisms remain elusive. we hypothesized that increased ca(2+)-activated k(+) (bk(ca)) channel activity contributes to the decreased myogenic tone of uterine arteries in pregnancy. resistance-sized uterine arteries were isolated from nonpregnant and near-term pregnant sheep. electrophysiological studies revealed a greater whole-cell k(+) current dens ... | 2011 | 22042813 |
| molecular characterization of a signal-regulated kinase homolog from echinococcus granulosus. | cystic echinococcosis due to echinococcus granulosus (e. granulosus) is one of the most important chronic helminthic diseases, especially in sheep/cattle-raising regions. the larval stage of the parasite forms a cyst that grows in the liver, lung, or other organs of the host. to ensure a long life in the host tissues, the parasite establishes complex inter-cellular communication systems between its host to allow its differentiation toward each larval stage. recent studies have reported that this ... | 2011 | 22040489 |
| A molecular survey on cystic echinococcosis in Sinnar area, Blue Nile state (Sudan). | Cystic echinococcosis (CE) is a zoonosis caused by the cestodes of the Echinococcus species. Its life cycle involves dogs and other canids as definitive hosts for the intestinal tapeworm, as well as domestic and wild ungulates as intermediate hosts for the tissue-invading metacestode (larval) stage. The disease has a special impact on disadvantaged pastoralist communities and is listed now among the three top priority neglected tropical disease (NTD). Therefore, CE is a neglected disease even in ... | 2011 | 22040487 |
| blood parasites of sheep in the netherlands. i. anaplasma mesaeterum sp.n. (rickettsiales, anaplasmataceae). | summary on two occasions an anaplasm was isolated from sheep on the dutch island of ameland. the organism proved to be highly pathogenic for splenectomised sheep; a non-splenectomised animal recovered spontaneously after the packed cell volume had decreased by 40%. treatment with oxytetracycline was effective. its pathogenicity for goats appeared to be low, and the organism was apparently not infective to splenectomised cattle. this anaplasm differs from anaplasma ovis in that less than 30% ... | 1979 | 22040472 |
| the effects of farming system and season on the prevalence of lameness in sheep in northwest iran. | to determine the prevalence of lameness in sheep in urmia, northwest iran, and compare the effects of production system and season on prevalence. | 2011 | 22040337 |
| dynamics of the free-living stages of sheep intestinal parasites on pasture in the north island of new zealand. 2. weather variables associated with development. | to identify weather variables associated with the development of eggs of teladorsagia (=ostertagia) circumcincta, trichostrongylus colubriformis and haemonchus contortus to third-stage infective larvae (l3) under a range of climatic conditions on pasture in the north island of new zealand. | 2011 | 22040333 |
| dynamics of the free-living stages of sheep intestinal parasites on pasture in the north island of new zealand. 1. patterns of seasonal development. | to describe the seasonal pattern of development of third-stage infective larvae (l3) from eggs of teladorsagia (=ostertagia) circumcincta, trichostrongylus colubriformis and haemonchus contortus on pasture in the north island of new zealand. | 2011 | 22040332 |
| Development and spatial distribution of the free-living stages of Teladorsagia circumcincta and Trichostrongylus colubriformis on pasture: a pilot study. | To measure the development of Teladorsagia (=Ostertagia) circumcincta and Trichostrongylus colubriformis eggs to third-stage infective larvae (L3) at different times of the year. Also, to measure the spatial distribution of L3 across herbage, soil and faeces, in order to assess whether spatial issues could be important in larval dynamics on pasture. | 2011 | 22040331 |
| caprine prion gene polymorphisms are associated with decreased incidence of classical scrapie in goat herds in the united kingdom. | abstract: the application of genetic breeding programmes to eradicate transmissible spongiform encephalopathies in goats is an important aim for reasons of animal welfare as well as human food safety and food security. based on the positive impact of prnp genetics on sheep scrapie in europe in the past decade, we have established caprine prnp gene variation in more than 1100 goats from the united kingdom and studied the association of prnp alleles with disease phenotypes in 150 scrapie-positive ... | 2011 | 22040234 |
| horse anti-bovine lymphocyte serum. its effect in calves. | summary anti-bovine lymphocyte serum (abls) had been prepared in horses with calf thymocytes as antigen and its effects in calves following parenteral administration were studied. the optimal dose was found to be one ml/kg body weight. the a bls suppressed both the t and b cell functions. the former was indicated by the disturbed response to sheep erythrocytes injections, by the decreased number of spontaneous e rosette forming lymphocytes, the prolonged survival of skin allografts and the si ... | 1980 | 22039984 |
| pasteurella haemolytica infections in sheep. | summary pasteurella haemolytica causes two distinct disease syndromes in sheep. p. haemolytica biotype a causes septicaemia in young lambs and pneumonia in all ages of sheep. biotype t produces an acute systemic disease affecting principally the upper alimentary tract and lungs in young adult sheep. the bacteriology, epidemiology and clinical and necropsy findings of the two diseases are described and the current situation regarding their experimental reproduction and immunology is reviewed. | 1980 | 22039979 |
| dna methylation status in tissues of sheep clones. | genomic imprinting and dna methylation play an important role in mammalian development. many cloned animals showed heterogeneous dna methylation profiles. however, there are fewer reports in cloned lambs because of a lack of genomic imprinting information. in this study, we investigated dna methylation patterns in cpg islands and differentially methylated regions of putative imprinted gene peg10 and imprinted genes dlk1, igf2r and h19 in cloned lambs. five organs from two cloned lambs died short ... | 2011 | 22039959 |
| torsion of the abomasum in ruminants: diagrammatic representation of rotary movements based on post-mortem findings. | summary a theoretical analysis of the types of displacement of the abomasum in the abdomen is presented based on a stud of forty-seven post-mortem examinations of calves, cows, lambs and sheep showing right-sided abomasal displacement and production of knots. these displacements are quantified following the introduction of a dual-axial system. one system (xyz) related to displacements of the abomasum on a pendulum model, the point of suspension being situated on the visceral surface of the li ... | 1980 | 22039933 |
| on some factors influencing the output of the larvae of prostrongylids of sheep in natural infections. | summary the output of first stage protostrongvlid larvae of sheep was studied in natural infections. three sets of observations were obtained from october 1977 to december 1978 in three regions. the larval output varied with the species harboured by sheep and with the density of worm populations. anthelmintic treatments influenced the fecundity of worms. the age of the animals was an important cause of variation in the size of larval output; a rise of the latter was observed in pregnant ewes ... | 1980 | 22039905 |
| effect of zinc and molybdenum supplementation of the feed concentrate on the storage of copper in the liver of lambs. | summary during a 98-day trial, eight groups of eight male weanling texel x friesian milk sheep lambs were fed the same hay and concentrates, the latter differing only in the contents of cu, zn, and mo. at high (± 30 ppm) and moderately low (± 15 ppm) copper levels in the concentrates, additions of zn (respectively 0, 150, and 340 ppm) and mo (0 and 16 ppm) to the concentrates were studied with regard to effect on the accumulation of copper in the liver. at the higher copper level, the additi ... | 1980 | 22039901 |
| blood parasites of sheep in the netherlands. ii. babesia motasi (sporozoa, babesiidae). | summary a large babesia species occurs in sheep on the north sea islands of the netherlands. the tick haemaphysalis punctata is a vector. its pathogenicity appears to be low. it is morphologically similar to a turkish strain, considered to be b. motasi, which is also transmitted by haemaphysalis ticks. it differs from the turkish parasite serologically as well as in cross-immunity tests and in not being effective to goats. there may be a group of morphologically similar parasites with ser ... | 1980 | 22039853 |
| the activity of albendazole against adult and larval gastrointestinal nematodes in naturally infected calves in the netherlands. | summary the activity of albendazole against gastrointestinal helminths in naturally infected calves in the netherlands was tested. the calves were in their fist grazing season and kept in two groups of ten. one of these groups was grazed alternately with sheep. five out of each group were drenched with albendazole (7.5 mg/kg) on the day they were housed (november 1). before and 2, 14, and 28 days after treatment individual faecal samples were taken from all calves and larval cultures were mad ... | 1979 | 22039826 |
| whole-genome sequence of corynebacterium pseudotuberculosis pat10 strain isolated from sheep in patagonia, argentina. | in this work, we report the complete genome sequence of a corynebacterium pseudotuberculosis pat10 isolate, collected from a lung abscess in an argentine sheep in patagonia, whose pathogen also required an investigation of its pathogenesis. thus, the analysis of the genome sequence offers a means to better understanding of the molecular and genetic basis of virulence of this bacterium. | 2011 | 22038974 |
| lamb survival analysis from birth to weaning in iranian kermani sheep. | survival records from 1,763 kermani lambs born between 1996 and 2004 from 294 ewes and 81 rams were used to determine genetic and non-genetic factors affecting lamb survival. traits included were lamb survival across five periods from birth to 7, 14, 56, 70, and 90 days of age. traits were analyzed under weibull proportional hazard sire models. several binary analyses were also conducted using animal models. statistical models included the fixed class effects of sex of lamb, month and year of bi ... | 2011 | 22038491 |
| immunodetection of aquaporin 5 in sheep salivary glands related to pasture vegetative cycle. | mammalian aquaporins (aqps) are a family of at least 13 integral membrane proteins expressed in various epithelia, where they function as channels to permeate water and small solutes. aqp5 is widely expressed in the exocrine gland where it is likely involved in providing an appropriate amount of fluid to be secreted with granular contents. as regards aqp5 expression in the salivary glands, literature is lacking concerning domestic animal species. this study was chiefly aimed at immunohistochemic ... | 2011 | 22038226 |
| diversity and abundance of the rumen and fecal methanogens in altay sheep native to xinjiang and the influence of diversity on methane emissions. | this study aims to investigate the influence of diet roughage proportion on the methanogenic communities from the rumen and fecal samples in altay local sheep native to xinjiang and better understand the association of methanogenic diversity or abundance with methane emissions of the ruminants. in this study, the high roughage diet was found to cause more methane emissions for either maintenance or ad-lib group, but the total methanogenic abundance was not influenced by roughage proportion and s ... | 2011 | 22038025 |
| Mitral Valve Annuloplasty : A Quantitative Clinical and Mechanical Comparison of Different Annuloplasty Devices. | Mitral valve annuloplasty is a common surgical technique used in the repair of a leaking valve by implanting an annuloplasty device. To enhance repair durability, these devices are designed to increase leaflet coaptation, while preserving the native annular shape and motion; however, the precise impact of device implantation on annular deformation, strain, and curvature is unknown. In this article, we quantify how three frequently used devices significantly impair native annular dynamics. In con ... | 2011 | 22037916 |
| nitric oxide-generating silicone as a blood-contacting biomaterial. | coagulation upon blood-contacting biomaterials remains a problem for short- and long-term clinical applications. this study examined the ability of copper(ii)-doped silicone surfaces to generate nitric oxide (no) and locally inhibit coagulation. silicone was doped with 3-μm copper [cu(0)] particles yielding 3 to 10 weight percent (wt%) cu in 70-μm thick cu/silicone polymeric matrix composites (cu/si pmcs). at 3, 5, 8, and 10 wt% cu doping, the surface expression of cu was 12.1% ± 2.8%, 19.7% ± 5 ... | 2011 | 22036723 |
| Contrasting patterns of population genetic structure of Fasciola hepatica from cattle and sheep: Implications for the evolution of anthelmintic resistance. | Twelve polymorphic genetic markers, eight allozymic loci and four microsatellites, were used to characterize 20 infrapopulations of Fasciola hepatica (all flukes from 10 individual cattle and 10 sheep) from 11 farms in Northwest Spain. Results suggest different patterns of population genetic structure depending on the host species. Individuals identified as clones were much more frequent in sheep. The common presence of clones and its nonrandom occurrence among individual hosts suggests clumped ... | 2012 | 22036705 |
| role of arginine-56 within the structural protein vp3 of foot-and-mouth disease virus (fmdv) o1 campos in virus virulence. | fmdv o1 subtype undergoes antigenic variation under diverse growth conditions. of particular interest is the amino acid variation observed at position 56 within the structural protein vp3. selective pressures influence whether histidine (h) or arginine (r) is present at this position, ultimately influencing in vitro plaque morphology and in vivo pathogenesis in cattle. using reverse genetics techniques, we have constructed fmdv type o1 campos variants differing only at vp3 position 56, possessin ... | 2012 | 22036313 |
| Blood Pb and d-ALAD inhibition in cattle and sheep from a Pb-polluted mining area. | The effects of Pb pollution on cattle and sheep raised in an ancient mining area were studied through the use of blood Pb (PbB) levels and d-aminolevulinic acid dehydratase (d-ALAD) activity. Lead levels in livestock blood from the mining area (n=110) were significantly elevated when compared to the controls (n=79). In 91.4% of cattle (n=58) and 13.5% of sheep (n=52) sampled in the mining area, PbB levels corresponded to subclinical exposure (6-35 µg/dl). Two young cattle (<2 years) from the min ... | 2012 | 22035934 |
| Doramectin resistance in Haemonchus contortus on an alpaca farm in Belgium. | Parasitism by gastrointestinal nematodes is a health concern in New World Camelids (NWC) worldwide, and anthelmintic treatment is often needed for parasite control. Although anthelmintic resistance has been reported in ruminants worldwide, data in NWC are only scarce. In the present study, a case of suspected doramectin resistance in alpacas was examined. A field efficacy study was conducted for the evaluation of two different dosages of doramectin using a faecal egg count reduction test. A grou ... | 2011 | 22035850 |
| Paratuberculosis control: a review with a focus on vaccination. | ABSTRACT: Mycobacterium avium subsp. paratuberculosis (MAP) infection causes in ruminants a regional chronic enteritis that is increasingly being recognized as a significant problem affecting animal health, farming and the food industry due to the high prevalence of the disease and to recent research data strengthening the link between the pathogen and human inflammatory bowel disease (IBD). Control of the infection through hygiene-management measures and test and culling of positive animals has ... | 2011 | 22035107 |
| [Structure, function and molecular design strategies of antibacterial peptide SMAP-29: a review]. | Antibacterial peptides are a family of host-defense peptides most of which are gene-encoded and produced by living organisms of all types. Antibacterial peptides are small molecular proteins with broad antimicrobial spectrum against bacteria, viruses, fungi and sometimes even as anticancer peptide. SMAP-29, a cathelicidin-like peptide derived from sheep myeloid, line alpha-helical Structure, exerts a powerful broad antimicrobial activity against different pathogens including Gram-positive and Gr ... | 2011 | 22034813 |
| high-resolution in utero 3d mr imaging of inner ear microstructures in fetal sheep. | developmental inner ear abnormalities can occur due to embryopathies as well as in the context of syndromal diseases like the charge association. in severe cases, an early and definite in utero diagnosis is important for decision-making; here, fetal mr imaging can be a helpful tool. we present results of performing high-resolution mr imaging of the inner ear structures of fetal sheep in vivo. | 2011 | 22033721 |
| identification of a novel astrovirus in domestic sheep in hungary. | the family astroviridae consists of two genera, avastrovirus and mamastrovirus, whose members are associated with gastroenteritis in avian and mammalian hosts, respectively. we serendipitously identified a novel ovine astrovirus in a fecal specimen from a domestic sheep (ovis aries) in hungary by viral metagenomic analysis. sequencing of the fragment indicated that it was an orf1b/orf2/3'utr sequence, and it has been submitted to the genbank database as ovine astrovirus type 2 (oastv-2/hungary/2 ... | 2011 | 22033597 |
| high resolution melting pcr to differentiate mycobacterium avium subsp. paratuberculosis"cattle type" and "sheep type". | in this study, we describe a novel hrm-pcr assay that clearly differentiates the two main types of mycobacterium avium subsp. paratuberculosis (cattle and sheep) based on the polymorphic variation of a previously described tandem repeat. this modern genotyping technique has several advantages over alternative methods, including cost, ease of use and rapidity. | 2012 | 22033498 |
| effects of phase cancellation and receiver aperture size on broadband ultrasonic attenuation for trabecular bone in vitro. | phase cancellation in ultrasound due to large receiver size has been proposed as a contributing factor to the inaccuracy of estimating broadband ultrasound attenuation (bua), which is used to characterize bone quality. transducers with aperture size ranging from 2 to 5 mm have been used in previous attempts to study the effect of phase cancellation. however, these receivers themselves are susceptible to phase cancellation because aperture size is close to one center wavelength (about 3 mm at 500 ... | 2011 | 22033134 |
| Effects of dietary rosemary extract on lamb spoilage under retail display conditions. | A dietary rosemary extract (RE) was tested to extend the shelf life of raw lamb meat. Lambs were supplemented with 0.6mgkg(-1) RE during fattening (from 13 to 25kg live weight). Meat spoilage (total viable counts, psycrophilic bacteria, Enterobacteriaceae, molds and yeasts), TBARS, CIE L*a*b* color and the sensory traits of lamb cuts were analyzed on days 0, 7, 14 or 21 under retail display conditions (70/30 O(2)/CO(2) atmosphere, 2°C temperature and 1600lx lighting). Supplementation of the lamb ... | 2012 | 22032920 |
| viral expression directs the fate of b cells in bovine leukemia virus-infected sheep. | the host immune response is believed to tightly control viral replication of deltaretroviruses such as human t-lymphotropic virus type 1 (htlv-1) and bovine leukemia virus (blv). however, this assumption has not been definitely proven in vivo. in order to further evaluate the importance of the immune response in the blv model, we studied the fate of cells in which viral expression was transiently induced. using a dual fluorochrome labeling approach, we showed that ex vivo induction of viral expr ... | 2012 | 22031946 |
| complete genome characterisation of a novel 26th bluetongue virus serotype from kuwait. | bluetongue virus is the "type" species of the genus orbivirus, family reoviridae. twenty four distinct bluetongue virus (btv) serotypes have been recognized for decades, any of which is thought to be capable of causing "bluetongue" (bt), an insect-borne disease of ruminants. however, two further btv serotypes, btv-25 (toggenburg orbivirus, from switzerland) and btv-26 (from kuwait) have recently been identified in goats and sheep, respectively. the btv genome is composed of ten segments of linea ... | 2011 | 22031822 |
| defining the differential sensitivity to norepinephrine and angiotensin ii in the ovine uterine vasculature. | the intact ovine uterine vascular bed (uvb) is sensitive to α-agonists and refractory to angiotensin ii (ang ii) during pregnancy; the converse occurs in the systemic circulation. the mechanism(s) responsible for these differences in uterine sensitivity are unclear and may reflect predominance of nonconstricting at(2) receptors (at(2)r) in uterine vascular smooth muscle (uvsm). the contribution of the placental vasculature also is unclear. third generation and precaruncular/placental arteries fr ... | 2012 | 22031783 |
| Sensitive and specific detection of sporadic Creutzfeldt-Jakob disease brain prion protein using real-time quaking induced conversion. | Real-time quaking induced conversion (RT-QuIC) is an assay in which disease-associated prion protein initiates a rapid conformational transition in recombinant prion protein resulting in the formation of amyloid that can be monitored in real time using the dye thioflavin T. It therefore has potential advantages over analogous cell-free prion protein conversion assays such as protein misfolding cyclic amplification (PMCA). The QuIC assay and the related amyloid seeding assay have largely been dev ... | 2011 | 22031526 |
| epidemiological factors related to the transmission risk of trypanosoma cruzi in a quilombola community, state of mato grosso do sul, brazil. | this work was an epidemiological investigation of the risk of trypanosoma cruzi transmission in the rural quilombola community of furnas do dionízio, state of mato grosso do sul, brazil. | 2011 | 22031072 |
| analysis of nasopharyngeal carcinoma risk factors with bayesian networks. | we propose a new graphical framework for extracting the relevant dietary, social and environmental risk factors that are associated with an increased risk of nasopharyngeal carcinoma (npc) on a case-control epidemiologic study that consists of 1289 subjects and 150 risk factors. | 2012 | 22030460 |
| evaluation of antioxidant status and oxidative stress in sheep naturally infected with babesia ovis. | the present study aimed to assess antioxidant status and oxidative stress in sheep naturally infected with babesia ovis. red blood cell (rbc) count, hemoglobin (hb) concentration, packed cell volume (pcv), piroplasm parasitemia percentage, malondialdehyde (mda) concentration, erythrocyte superoxide dismutase (sod), glutathione peroxidase (gsh-px), catalase (cat) activities and total antioxidant capacity (tac) were determined in 52 sheep naturally infected with b. ovis as well as same number of h ... | 2011 | 22030375 |
| prion protein self-interaction in prion disease therapy approaches. | transmissible spongiform encephalopathies (tses) or prion diseases are unique disorders that are not caused by infectious micro-organisms (bacteria or fungi), viruses or parasites, but rather seem to be the result of an infectious protein. tses are comprised of fatal neurodegenerative disorders affecting both human and animals. prion diseases cause sponge-like degeneration of neuronal tissue and include (among others) creutzfeldt-jacob disease in humans, bovine spongiform encephalopathy (bse) in ... | 2011 | 22029882 |
| small calibre biosynthetic bacterial cellulose blood vessels: 13-months patency in a sheep model. | abstract objectives. many patients in need of bypass surgery lack graft material and current synthetic alternatives have poor performance. a 4 mm vascular graft composed of bacterial cellulose (bc) was developed and tested in pilot study in a large animal model. design. bc is a biopolymer made by the bacteria acetobacter xylinum. bc grafts (n = 16) with 4 cm length and 4 mm internal diameter were implanted bilaterally in the carotid arteries of eight sheep. no long-term antithrombotic therapy ... | 2011 | 22029845 |
| Copper-induced hepatitis: the COMMD1 deficient dog as a translational animal model for human chronic hepatitis. | Chronic inflammatory liver disease regardless of aetiology leads to failing regeneration and fibrosis, ending in cirrhosis. Both in man and in animals this worldwide health problem has no definitive cure. Chronic liver injury causes hepatic stellate cells to proliferate and differentiate into matrix-producing cells. New therapeutic options will be developed upon detailed understanding of the molecular mechanisms driving liver fibrosis. This may lead to new anti-fibrotic therapies which need to b ... | 2011 | 22029820 |
| the molecular architecture for the intermediate filaments of hard α-keratin based on the superlattice data obtained from a study of mammals using synchrotron fibre diffraction. | high- and low-angle x-ray diffraction studies of hard α-keratin have been studied, and various models have been proposed over the last 70 years. most of these studies have been confined to one or two forms of alpha keratin. this high- and low-angle synchrotron fibre diffraction study extends the study to cover all available data for all known forms of hard α-keratin including hairs, fingernails, hooves, horn, and quills from mammals, marsupials, and a monotreme, and it confirms that the model pr ... | 2011 | 22028970 |
| mapuche herbal medicine inhibits blood platelet aggregation. | 12 plant species traditionally used by the mapuche people in chile to treat wounds and inflammations have been evaluated for their direct blood platelet inhibition. seven of the 12 tested plant species showed platelet inhibitory effect in sheep blood, and four of these were also able to inhibit the adp- (5.0 μm) and collagen- (2.0 μg/ml) induced aggregations in human blood. these four species in respective extracts (in brackets) were blechnum chilense (meoh), luma apiculata (h(2)o), amomyrtus lu ... | 2012 | 22028732 |
| Effect of maternal age and growth on placental nutrient transport: potential mechanisms for teenagers' predisposition to small-for-gestational-age birth? | Teenagers have an increased risk of delivering small-for-gestational-age (SGA) infants. Young maternal age and continued skeletal growth have been implicated as causal factors. In growing adolescent sheep, impaired placental development and nutrient transfer cause reduced birth weight. In human pregnancies, SGA is associated with reduced placental amino acid transport. Maternal growth has no effect on placental morphology or cell turnover, but growing teenagers have higher birth weight:placental ... | 2011 | 22028413 |
| ultrahigh resolution and full-length pilin structures with insights for filament assembly, pathogenic functions, and vaccine potential. | pilin proteins assemble into type iv pili (t4p), surface-displayed bacterial filaments with virulence functions including motility, attachment, transformation, immune escape, and colony formation. however, challenges in crystallizing full-length fiber-forming and membrane protein pilins leave unanswered questions regarding pilin structures, assembly, functions, and vaccine potential. here we report pilin structures of full-length dnfima from the sheep pathogen dichelobacter nodosus and ftpile fr ... | 2011 | 22027840 |
| [brabants knowledge network zoonoses: health is not small change]. | 2011 | 22026226 | |
| analysis and comparison of proteomic profiles of tear fluid from human, cow, sheep, and camel eyes. | to investigate the tear proteome profiles of human, cow, sheep, and camel comparatively and to explore the difference of tear protein profiles among different species. | 2011 | 22025569 |
| augment® bone graft products compare favorably to autologous bone graft in an ovine model of lumbar interbody spine fusion. | study design. this study was designed to determine whether augment bone graft (augment) and augment injectable bone graft (augment injectable), two combination devices comprised of human recombinant platelet-derived growth factor-bb (rhpdgf-bb) and β-tricalcium phosphate (tcp)-containing matrices, promote bone bridging in an ovine model of lumbar spine fusion. autologous bone graft (autograft) was used as a positive control.objective. the purpose of this study was to determine the ability of aug ... | 2011 | 22024900 |
| Control of paratuberculosis in small ruminants. | The clinical presentation of paratuberculosis in small ruminants is unthriftiness (poor body condition); severe diarrhea is not a common clinical sign. In the USA, goats are primarily infected with bovine strains of paratuberculosis and sheep are primarily infected with ovine strains. Because ovine strains cannot be easily cultured, confirmation of a diagnosis is best done by polymerase chain reaction on tissue or fecal samples. Control programs must be tailored to the business objectives of the ... | 2011 | 22023839 |
| Coxiella burnetii, the Agent of Q Fever, in Domestic Sheep Flocks from Wyoming, United States. | Abstract Coxiella burnetii, the agent of Q fever, is an intracellular bacterial pathogen. It has a nearly cosmopolitan distribution. We conducted a serological survey of domestic sheep herds for infections with C. burnetii in Wyoming following reports of abortion and open ewes. Based on the serologic evidence, there was no link between reproductive problems and exposure to C. burnetii. However, the overall prevalence of C. burnetii in WY sheep was 7%, which indicates that the agent is present ... | 2011 | 22022809 |
| chromosomal aberrations in ovine lymphocytes exposed in vitro to tolylfluanid. | chromosomal aberrations have been used as important cytogenetic biomarkers to study the mutagenic effects of different chemicals in vivo and in vitro. chromosomal aberrations were evaluated in cultures of sheep lymphocytes in vitro exposed to the fungicide tolylfluanid. lymphocyte cultures from three donors were exposed to four different concentrations of fungicide (1.10(-4) m(.)l; 1.10(-5) m(.)l; 1.10(-6) m(.)l; 1 × 10(-7) m(.)l). chromosomal analysis showed a significant (p = 0.018 and 0.038 r ... | 2012 | 22022782 |
| antistress activity of argyreia speciosa roots in experimental animals. | the antistress effect of a seven-day treatment (100 and 200 mg / kg, p.o.) of the hydroalcoholic extract of argyreia speciosa root (ase) was evaluated by using the swimming endurance test, acetic acid-induced writhing test, pentylenetetrazole-induced convulsion test, anoxic tolerance test, cold-restraint, stress-induced gastric ulcers, aspirin-induced ulcers, and biochemical, and histopathological changes in the cold-restraint stress test. the immunomodulatory activity was also evaluated for the ... | 2011 | 22022155 |
| Index selection in terminal sires improves early lamb growth. | The use of terminal sires (TS) for crossbreeding is integral to the UK sheep industry where approximately 71% of market lambs are sired by TS rams. Early growth of these crossbred lambs affects profitability. The objectives of this study were: (i) to evaluate the effectiveness of index selection among TS on the BW and ADG of their crossbred offspring; and, (ii) to compare the efficacy of that selection within TS breeds. The most widely used TS breeds in the UK are Charollais, Suffolk and Texel. ... | 2011 | 22021813 |
| Stable carbon isotope composition of perirenal adipose tissue fatty acids from Engadine sheep grazing either mountain or lowland pasture. | To provide further insights into ruminant lipid digestion and metabolism, and into cis-9,trans-11 18:2 synthesis, 12 growing Engadine lambs grazing either mountain pasture (2250 m above sea level; n = 6) or lowland pasture (400 m above sea level; n = 6) were studied. Both pastures exclusively consisted of C(3) plants. Before the experiment, all animals grazed a common pasture for 6 weeks. Grasses and perirenal adipose tissues of the sheep were analyzed for fatty acids by gas chromatography. Stab ... | 2011 | 22021810 |
| Opportunities for sheep vets in farm health planning. | 2011 | 22021259 | |
| Investigation of the presence of ESBL-producing Escherichia coli in the North Wales and West Midlands areas of the UK in 2007 to 2008 using scanning surveillance. | Between November 5, 2007 and November 4, 2008, faecal samples from cattle and sheep submitted for diagnostic purposes to the Aberystwyth and Shrewsbury Veterinary Laboratories Agency (VLA) (now AHVLA) regional laboratories (covering North Wales and the West Midlands) were screened for the presence of Escherichia coli that produces CTX-M extended-spectrum ß-lactamase (ESBL) using the selective medium CHROMagar CTX. Samples from 113 farms were tested and eight ESBL-positive farms identified. Of th ... | 2011 | 22021063 |
| Balloon occlusion of the right main bronchus in an ovine model provides sufficient time for emergent interventions in massive pulmonary embolism. | PURPOSE Massive pulmonary embolism (PE) causes hemodynamic compromise and is associated with a high rate of mortality. We sought to create a model of massive PE and to determine whether occlusion of the right main bronchus could mitigate the physiological effects of massive PE in this model. MATERIALS AND METHODS We used 27 female sheep to generate a model of massive PE by either autologous blood clot injection (n=18) or detachable balloon release (n=9) into the right mai ... | 2012 | 22020948 |
| Cloning and polymorphism analysis of prion protein gene in domestic bactrian camel in China. | Up to now, little is known about the prion protein gene (PRNP) of domestic bactrian camels, and no polymorphisms of the bactrian camel PRNP have been analyzed or reported. In this study, we cloned and analyzed the PRNP sequences of 89 domestic bactrian camels. The results showed that the amino acid sequence of bactrian camel PrP starts with the consensus sequence MVKSH, with almost identical amino acid sequence to the PrP of dromedary camels. A four octapeptide PHGGGWGQ repeat region follows a n ... | 2012 | 22019430 |
| characterization of pou5f1 (oct4) gene and its promoter in buffalo esc-like cells identifies multiple transcription start sites and expression of four pseudogenes. | in the present study, we cloned and characterized the buffalo (bubalus bubalis) oct4 ortholog expressed in embryonic stem cell (esc) like cells and its promoter region. the 5'- and 3'-race experiments were conducted to analyze the transcription initiation site and regulatory regions. the comparative analysis of buffalo oct4 promoter with other mammalian orthologs revealed high conservation. among the regulatory regions highest similarity was observed between buffalo, bovine and sheep. interestin ... | 2012 | 22019428 |
| single residues in the surface subunits of oncogenic sheep retrovirus envelopes distinguish receptor-mediated triggering for fusion at low ph and infection. | jaagsiekte sheep retrovirus (jsrv) and enzootic nasal tumor virus (entv) are two closely related oncogenic retroviruses that share the same cellular receptor yet exhibit distinct fusogenicity and infectivity. here, we find that the low fusogenicity of entv envelope protein (env) is not because of receptor binding, but lies in its intrinsic insensitivity to receptor-mediated triggering for fusion at low ph. distinct from jsrv, shedding of entv surface (su) subunit into culture medium was not enha ... | 2011 | 22018783 |
| prpsc detection in formalin-fixed paraffin-embedded tissue by elisa. | abstract: | 2011 | 22018205 |
| Immunotoxicological profile of chloramine in female B6C3F1 mice when administered in the drinking water for 28 days. | Monochloramine has been used to provide a disinfecting residual in water distribution systems where it is difficult to maintain an adequate free-chlorine residual or where disinfection by-product formation is of concern. The goal of this study was to characterize the immunotoxic effects of chloramine in female B(6)C(3)F(1) mice when administered via the drinking water. Mice were exposed to chloramine-containing deionized tap water at 2, 10, 20, 100, or 200 ppm for 28 days. No statistically signi ... | 2011 | 22017662 |
| klebsiella pneumoniae type 3 fimbriae agglutinate yeast in a mannose resistant manner. | the ability of bacterial pathogens to express different fimbrial adhesins plays a significant role in virulence. thus, specific detection of fimbrial expression is an important task in virulence characterization and epidemiological studies. most clinical klebsiella pneumoniae isolates express type 1 and type 3 fimbriae characterized by mediating mannose sensitive agglutination of yeast cells, and agglutination of tannic acid treated ox red blood cells (rbcs), respectively. we have observed that ... | 2011 | 22016558 |