Publications

influence of lectins on female sex pheromone reception byheterodera schachtii (nematoda: heteroderidae) males.	a bioassay was developed in which the chemotactic behavior of males of the sugarbeet cyst nematodeheterodera schachtii towards the female sex pheromone can be observed and documented at any time. the standardized test, performed under sterile conditions, gives reproducible results within 1-2 hr. incubation of males with the lectins fromcanavalia ensiformis, triticum vulgare, arachis hypogaea, helix pomatia, andlimax flavus did not affect chemotactic attraction to the sex pheromone. supplementing ...	1990	24264204
allelochemicals in soil from no-tillage versus conventional-tillage wheat (triticum aestivum) fields.	putative allelochemicals found in the soil of no-tillage and conventional-tillage wheat plots near stillwater, oklahoma, were obtained by a mild alkaline aqueous extraction procedure, bioassayed to determine their biological activity, purified, and analyzed with a capillary gas chromatography-mass spectrometry-data analysis system. the most significant inhibition was found in bioassays of extracts from soil collected immediately after harvest in june, july, and august. no-tillage soils produced ...	1990	24264093
devil's-claw (proboscidea louisianica), essential oil and its components : potential allelochemical agents on cotton and wheat.	the potential allelopathic activity of devil's-claw [proboscidea louisianica (mill.) thellung] essential oil and a few of the compounds it contains on the elongation of cotton (gossypium hirsutum l.) and wheat (triticum aestivum l.) radicles was studied using a petri dish bioassay. essential oil was collected by steam distillation using an all-glass-teflon assembly. ether extracts of the steam distillates from fresh devil's-claw were inhibitory to cotton and wheat radicle elongation. the followi ...	1990	24263996
the relationship between the rht 1 and rht 2 dwarfing genes and grain weight in triticum aestivum l. spring wheat.	the study was carried out in the first year on samples of random f5 lines, uniform in height and in heading date, of three crosses between semi dwarf spring wheat cultivars (triticum aestivum l.), differing in grain weight and in their rht gene. in the second year only the progenies of the early heading f5 lines were studied. all the material was grown in the absence of lodging. the culm-length genotypes of the different lines were identified by test crosses and by a seedling ga response test. n ...	1983	24263770
new hybrids between agropyron and wheat : 2. production, morphology and cytogenetic analysis of f1 hybrids and backcross derivatives.	intergeneric hybrids of triticum aestivum (2n=42,aabbdd) with agropyron ciliare (2n= 28,ssyy), a. trachycaulum (2n=28,sshh), a. yezoense (2n=28,ssyy) and a. scirpeum (2n=28) are reported for the first time. f1 hybrids of t. aestivum were also produced with a. intermedium (2n=42,e1e1e2e2z1z1) and a. junceum (2n=14,juju). all wheat-agropyron hybrids were obtained by embryo rescue technique. cultivars and reciprocal crosses differed for seed set, seed development and f1 plant production. the f1 hyb ...	1983	24263763
the response of anther culture to culture temperature in triticum aestivum.	the response of anther culture to culture temperature was studied in detail using many varieties, f1 hybrids and pollen-derived lines of wheat (triticum aestivum) as materials. the suitable culture temperature for inducing pollen callus (or embryoids) in wheat anther culture ranged from 26 °c to 30 °c, varying with genotypes. but for the great majority of wheat genotypes the suitable culture temperatures lay between 28 °c and 30°c. the most significant genotypic variation in the response to cult ...	1983	24263762
genetic control of endosperm proteins in wheat : 2. variation in high molecular weight glutenin and gliadin subunits of triticum aestivum.	endosperm protein subunits of 109 primitive and modern lines of hexaploid wheat, triticum aestivum l. em. thell., were fractionated by one-dimensional, high resolution, sodium dodecyl sulphate (sds) polyacrylamide gel electrophoresis (page). a wide range of both qualitative and quantitative variation was observed in the fractions of the high molecular weight (hmw) glutenin and gliadin subunits of the different lines. the qualitative variation was expressed in the number of subunits per fraction ...	1983	24263634
responses of alloplasmic (cytoplasm=triticum timopheevii) and euplasmic wheats (triticum aestivum) to photoperiod and vernalization.	studies were conducted to determine the influence of the male sterility-inducing cytoplasm of triticum timopheevii (zhuk.) zhuk. on response of several common winter wheat (t. aestivum l.) nuclear genotypes to photoperiod and vernalization. comparative studies of cytoplasmic substitution lines provide information on the role of the cytoplasmic genetic mechanism in growth and development. in the case of cytoplasmic male sterility-based hybrid production systems, ubiquity of sterility-inducing cyt ...	1983	24263632
the use of irradiated pollen for differential gene transfer in wheat (triticum aestivum).	the use of irradiated pollen to bring about limited gene transfer in wheat has been investigated. doses of x-rays of 2kr, 3kr and 5kr were used to generate m1 progeny between maternal and paternal genotypes differing in quantitative and major gene characters. cytological studies of m1 plants revealed hybrids with widespread aneuploidy and structural rearrangements in the paternal genome. these effects resulted in phenotypic variation between m1 progeny and complex multivalent formation at meiosi ...	1983	24263337
increasing grain protein content of hard red winter wheat (triticum aestivum l.) by mutation breeding.	poor adaptability or functional quality of much germplasm used for breeding high-protein hard red winter wheats prompted mutagenesis as an alternative means of increasing grain protein content. four hard red winter wheat genotypes - ks644 ('triumph// concho/triumph'), 'kaw', 'parker', and 'shawnee' - were treated with 0.40 m ethyl methanesulfonate (ems). advanced lines (m8-m10) were selected that had a 3-year mean grain protein advantage of 0.7% to 2.0% over controls. increased grain protein con ...	1983	24263199
generation of doubled haploid transgenic wheat lines by microspore transformation.	microspores can be induced to develop homozygous doubled haploid plants in a single generation. in the present experiments androgenic microspores of wheat have been genetically transformed and developed into mature homozygous transgenic plants. two different transformation techniques were investigated, one employing electroporation and the other co-cultivation with agrobacterium tumefaciens. different tissue culture and transfection conditions were tested on nine different wheat cultivars using ...	2013	24260351
sequence-based analysis of translocations and inversions in bread wheat (triticum aestivum l.).	structural changes of chromosomes are a primary mechanism of genome rearrangement over the course of evolution and detailed knowledge of such changes in a given species and its close relatives should increase the efficiency and precision of chromosome engineering in crop improvement. we have identified sequences bordering each of the main translocation and inversion breakpoints on chromosomes 4a, 5a and 7b of the modern bread wheat genome. the locations of these breakpoints allow, for the first ...	2013	24260197
difference in hydroxamic acid content in roots and root exudates of wheat (triticum aestivum l.) and rye (secale cereale l.): possible role in allelopathy.	hydroxamic acids (hx) produced by some cereal crops have been associated with allelopathy. however, the release of hx to the soil by the producing plant-an essential condition for a compound to be involved in allelopathy-has not been shown. gc and hplc analysis of roots and root exudates of wheat (triticum aestivum l.) and rye (secale cereale l.) cultivars, with high hx levels in their leaves, demonstrated the presence of these compounds in the roots of all cultivars analyzed and in root exudate ...	1991	24259166
production and characterization of alloplasmic lines of a triticale 'rosner'.	the transfer of cytoplasms of various triticum and aegilops species to a hexaploid triticale ('rosner') has been attempted using 30 alloplasmic lines and a euplasmic line of common wheat as cytoplasmic donors. the average rate of f1 hybrid production (seed setting ratexgermination rate) following an ordinary method of crossing is only 0.09%, whereas this rate is increased to 3.1% by use of embryo culture. the first backcross of the f1 plants with triticale pollen is again difficult, the hybrid p ...	1984	24258961
combining abilities and heritability of callus formation and plantlet regeneration in wheat (triticum aestivum l.) anther cultures.	frequency of callus formation in wheat (triticum aestivum l. em. thell) anthers cultured in vitro and the frequency of subsequent plantlet formation from such calli were examined in a diallel population produced from five inbred spring wheat cultivars. two of the five cultivars were believed to possess relatively high frequencies of response and the other three relatively low response frequencies, based on previous studies. general and specific combining abilities were estimated and found to be ...	1984	24258955
analysis of nucleolar activity in agropyron elongatum, its amphiploid with triticum aestivum and the chromosome addition lines.	the nucleolar organizer activity of the agropyron elongatum, its amphiploid with hexaploid wheat (triticum aestivum) and the chromosome addition lines is analyzed by the silver-staining procedure. four ag-nors are observed in a. elongatum corresponding to the chromosomes 6e and 7e. in the amphiploid t. aestivum - a. elongatum, eight ag-nors are observed which corresponds the wheat chromosomes 1b and 6b and to the elongatum chromosomes 6e and 7e. thus, there is codominance in the nucleolar organi ...	1984	24258946
heritability of grain yield, plant height and test weight of a population of winter wheat adapted to southwestern ontario.	grain yield, plant height and test weight were studied in a population of winter wheat (triticum aestivum l.). the population consisted of f2 bulk populations of 42 crosses among 11 genotypes adapted to s. w. ontario. heritabilities were: 0.30±0.32 for yield, 0.77±0.15 for height and 0.98±0.08 for test weight. predicted genetic gain with 10% selection intensity was 0.15 t/ha for yield, 10.1 cm for height and 3.00 kg/hl for test weight. the low heritability for yield indicates that effective sele ...	1984	24258938
nad-dependent aromatic alcohol dehydrogenase in wheats (triticum l.) and goatgrasses (aegilops l.): evolutionary genetics.	evolutionary electrophoretic variation of a nad-specific aromatic alcohol dehydrogenase, aadh-e, in wheat and goatgrass species is described and discussed in comparison with a nad-specific alcohol dehydrogenase (adh-a) and a nadp-dependent aadh-b studied previously. cultivated tetraploid emmer wheats (t. turgidum s. l.) and hexaploid bread wheats (t. aestivum s. l.) are all fixed for a heterozygous triplet, e(0.58)/e(0.64). the slowest isoenzyme, e(0.58), is controlled by a homoeoallelic gene on ...	1984	24258843
metabolism of activated oxygen in detached wheat and rye leaves and its relevance to the initiation of senescence.	the activities of several enzymes either generating or decomposing o 2 (-) or h2o2, were investigated during the course of senescence of detached wheat (triticum aestivum l.) and rye (secale cereale l.) leaves in light and in darkness. most of the activities, although not in full synchrony, declined with the degradation of chlorophyll and protein. the decline was slower in light than in darkness (e.g. glycolate oxidase, ec 1.1.3.1; urate oxidase, ec 1.7.3.3.; catalase, ec 1.11.1.6) and was furth ...	1984	24258664
cultivar and cultivar x environment effects on the development of callus and polyhaploid plants from anther cultures of wheat.	plants of three common wheat (triticum aestivum l. em. thell) cultivars and one randomly selected doubled-haploid line derived by anther culture from each of the three cultivars were each grown in three environments, a field environment, a greenhouse environment, and a growth chamber environment. anthers containing largely miduninucleate to late uninucleate microspores were cultured and calli were induced to regenerate plants in order to assess the effects of cultivar, cultivar family (cultivar ...	1984	24258560
identification of c-banded chromosomes in meiosis of common wheat, triticum aestivum l.	the c-banding pattern of nine meiotic chromosomes of common wheat (triticum aestivum l.) as described. in f1s of crosses between monosomics of 'chinese spring' and two spanish wheat cultivars, univalent chromosomes were used to aid the recognition and analysis of the c-banding pattern for the individual chromosomes. the identification of one chromosome involved in one translocation in 'chinese spring' x 'pané 247' has been made through heterochromatin bands observed in the chromosomes involved i ...	1984	24258557
chromosome variation in wheat plants regenerated from cultured immature embryos.	a cytological study has been made of plants regenerated from cultured immature embryos of four wheat cultivars (triticum aestivum, 2n = 6x = 42). in total, 29% of the 192 plants examined were aneuploid with a range in chromosome numbers of 38-45. evidence of chromosome structural changes was also found. this variation occurred in regenerants of all four cultivars, but there were large differences in the proportions of aneuploids arising from individual cultures which meant that no significant di ...	1984	24258556
metabolism of r,s-[2-(14)c]abscisic acid by non-stressed and water-stressed detached leaves of wheat (triticum aestivum l.).	metabolism of r,s-[2-(14)c]abscisic acid (aba) was studied in detached leaves of six wheat (triticum aestivum) cultivars, using non-stressed leaves or leaves water stressed by desiccation to 90% of their original fresh weight. the rate constant of aba metabolism was similar in nonstressed leaves of all cultivars. water stress resulted in significantly lower rate constants in two cultivars which accumulated high levels of aba when stressed, the constants decreasing by a factor of about 1.5. rate ...	1984	24258508
control of lectins in triticum aestivum and aegilops umbellulata by homoeologous group 1 chromosomes.	each of the three genomes in hexaploid wheat controls the expression of a specific lectin in the embryo. the chromosomes which control their synthesis were determined using nullisomic-tetrasomic and inter-varietal chromosome substitution lines of 'chinese spring'. all three wheat lectins were shown to be controlled by the homoeologous group 1 chromosomes. using ditelosomic lines of 'chinese spring' the lectin genes could be localized on the long arms of chromosomes 1a and 1d. inter-specific addi ...	1983	24258480
thylakoid protein kinase activity and associated control of excitation energy distribution during chloroplast biogenesis in wheat.	the activity of thylakoid protein kinase and the regulation of excitation energy distribution between photosystems i and ii was examined during chloroplast biogenesis in light-grown triticum aestivum (wheat) leaves. the specific activity of the thylakoid protein kinase decreased some six-fold during development from the young plastids at the base of the 7-d-old leaf to the mature chloroplasts at the leaf tip. appreciable activity was also detected in plastids isolated from etiolated leaves. in m ...	1983	24258136
isolation and characterization of dna sequences from triticum aestivum which function as origins of replication in saccharomyces cerevisiae.	recombinant yip5 plasmids with the dna from triticum aestivum are capable of autonomous replication in saccharomyces cerevisiae. the ura transformants are unstable without selection pressure, and transformation of yeast cells with these plasmids occurs at high frequency. the cloned sequences were characterized and analyzed to state their belonging to triticum tribe.	1983	24258044
chromosomal assignment of genes coding for the wheat gliadin protein components of the cultivars 'cheyenne' and 'chinese spring' by two-dimensional (two-ph) electrophoresis.	the gliadin proteins of the hexaploid bread wheat cultivar 'cheyenne' (triticum aestivum l. var. aestivum), which has good mixing and baking characteristics, were analyzed by 2-dimensional (2-ph) polyacrylamide gel electrophoresis (ph 3.2 in the 1st dimension, ph 9.2 in the 2nd). genes for most of the 35 resolved components were assigned to the chromosomes of homoeologous groups 1 and 6 through the use of various aneuploids and substitution lines. a similar analysis was carried out for the culti ...	1984	24257826
in vitro synthesis of lectins in cell-free extracts from dry wheat and rye embryos.	cell-free extracts from dry wheat (triticum vulgare l.) and rye (secale cereale l.) embryos do not synthesize their corresponding lectins when incubated under conditions optimalized for translation of either exogenous or endogenous mrna. only when the extracts are prepared and incubated in the complete absence of sulphydryl containing compounds lectins are synthesized in vitro. since immunoprecipitation techniques could not demonstrate unequivocally the presence of lectin among the cell-free tra ...	1982	24257711
somatic embryogenesis and plant regeneration in callus from inflorescences of hordeum vulgare x triticum aestivum hybrids.	embryogenic callus cultures were obtained by culturing young inflorescence tissues of hordeum vulgare cv. 'pf51811' (2x)xtriticum aestivum cv. 'chinese spring' (6x) hybrids on 2,4-d-containing n6 medium. after subculture for about 10 months the calli retained a high potentiality for somatic embryogenesis and plant regeneration. of about 300 regenerated plants, approximately 100 were transplanted to potting soil. eight embryoids and three regenerated plants examined had 28 chromosomes identical t ...	1984	24257648
study on nuclear and cytoplasmic genome expression in wheat by two-dimensional gel electrophoresis : 2. genetic differences between two lines and two groups of cytoplasms at five developmental stages or organs.	two-dimensional gel electrophoresis of denaturated proteins were performed at five developmental stages or organs (hereafter referred to as stage-organs) on two wheat lines with four different cytoplasms. five hundred and fifty to 712 reproducible spots were scored depending on the stage-organ. each stage-organ is unambiguously characterized and several types of control of protein quantity are recorded. post-translational modifications are hypothetized and may sometimes be stagespecific. two cyt ...	1984	24257643
development and characterisation of a line of bread wheat, triticum aestivum, which lacks the short-arm satellite of chromosome 1b and the gli-b1 locus.	about 360 offspring of a tri-parental cross were screened by gel electrophoresis and unexpectedly one of them did not contain chromosome 1b ω-gliadins derived from either of the primary parents. a line disomic for the ω-gliadin null was developed from the surviving embryo half of the unique grain. two dimensional electrophoresis revealed that all the storage protein genes at gli-b1, coding for γ-gliadins, β-gliadins and low-molecular-weight subunits of glutenin as well as the ω-gliadin, were not ...	1984	24257642
a new approach for estimating the phytotoxicity limits.	a pot experiment was carried out on a typic ustipsamment to study the effect of cd concentration on the yield of wheat (triticum aestivum) and soybean (glycine max). cd levels taken were 1, 5, 10, 20, 40, 80, and 160 μg g(-1) of soil. three different statistical procedures were employed to evaluate the phytotoxicity limits. the non-linear regression technique was found to be more effective in calculating c 0 (threshold concentration) and c 100 (toxic concentration) in comparison to cate and nels ...	1987	24254227
the influence of temperature on chromosome elimination during embryo development in crosses involving hordeum spp., wheat (triticum aestivum l.) and rye (secale cereale l.).	several interspecific and intergeneric crosses involving five hordeum species, triticum aestivum and secale cereale were carried out to investigate the influence of two contrasting temperatures on chromosome elimination during embryo development. in four of the interspecific hordeum crosses, chromosome elimination was significantly increased at the higher of the two temperatures, resulting in greater proportions of haploid plant progenies. however, there was no significant effect of temperature ...	1985	24254180
the chromosome constitution of plants derived from pollen of hexaploid triticale x common wheat f1 hybrids.	the anthers of three f1 hybrids of hexaploid triticale and common wheat ('rosner' x 'kedong 58', 'beagle' x 'kedong 58' and 'beagle' x 'jinghua no. 1') were cultured on four media in vitro. more than 900 green plants were obtained. the chromosome numbers ranged from 17 to 27 for haploid derivatives and from 38 to 52 for diploid regenerates. the chromosome constitutions of the pollen plants reflect those of the gametes found in the donor plants (genome formula: aabbdr). the value of such pollen p ...	1985	24254120
effect of the 1b/1r translocation on anther culture ability in wheat (triticum aestivum l.).	using two varieties, their reciprocal hybrids, f8 lines and doubled haploids, results confirmed that three genetic components are involved in wheat anther culture ability, viz embryo induction frequency, regeneration ability and the frequency of albinism. in these experiments, no significant maternal effects were noticed. for embryo yields, transgressive lines were obtained from hybrids between distant genotypes. regeneration of green plants depended upon two independent traits: regeneration abi ...	1985	24254069
genetic control of endosperm proteins in wheat : 3. allocation to chromosomes and differential expression of high molecular weight glutenin and gliadin genes in intervarietal substitution lines of common wheat.	total endosperm proteins extracted from both several common wheat cultivars and some intervarietal substitution lines derived from them were fractionated according to their molecular weight in a high resolution one-dimensional gel electrophoresis. the four donor cultivars and the recipient one - 'chinese spring', possessed differentially migrating protein bands in the fractions of high molecular weight (hmw) glutenins and gliadins. several of these bands were identified for the first time in thi ...	1985	24254017
characterization of a wheat germ agglutinin-like lectin from adult wheat plants.	radioimmuno-and enzyme-linked immunosorbent assays show that a substantial amount of wheat germ agglutinin(wga)-like protein is present at the base of the shoot and in the roots of adult wheat (triticum aestivum l.) plants. the protein can be purified by hapten-and antibody-mediated affinity procedures. it forms an arc of identity with the embryo lectin upon ouchterlony double-diffusion and is an active lectin that agglutinates trypsinized erythrocytes in an n-acetylglucosamine-and chitin-inhibi ...	1984	24253947
role of d-genome chromosomes in photosynthesis expression in wheats.	the role of d-genome chromosomes in the expression of net photosynthesis in wheats was analysed with the nullitetrasomic and ditelosomic lines of the bread wheat cultivar 'chinese spring'. the two arms of chromosome 3 d and the short arm of chromosome 6 d control major mechanisms of photosynthesis. the effect of chromosome 6 d can be thoroughly compensated by that of its homoeologues of genomes a or b, contrary to what can be observed for chromosome 3 d. chromosome 7 d is responsible for the low ...	1985	24253914
the effect of additions ofaegilops longissima chromosomes on grain protein in common wheat.	the effect of various chromosomes ofaegilops longissima when added to the common wheat cultivar 'chinese spring' was evaluated at two levels of nitrogen fertilization for absolute and relative amount of protein in the grain. all the added chromosomes ofae. longissima increased protein percentage: protein increase by chromosomes d, c and a averaged 3.8% while that by chromosomes f, e, g and b averaged 1.7%. addition lines f, d and c had a significantly higher protein weight per grain. on the othe ...	1985	24253912
growth and gibberellin-a1 metabolism in normal and gibberellin-insensitive (rht3) wheat (triticum aestivum l.) seedlings.	growth parameters were determined for tall (rht3) and dwarf (rht3) seedlings of wheat (triticum aestivum l.). plant statures and leaf length were reduced by 50% in dwarfs but root and shoot dry weights were less affected. leaves of dwarf seedlings had shorter epidermal cells and the numbers of cells per rank in talls and dwarfs matched the observed relationships in overall length. talls grew at twice the rate of dwarfs (2.3 compared with 1.2 mm h(-1)). [(3)h]gibberellin a1 ([(3)h]ga1) was fed to ...	1984	24253843
comparative genetic analyses of metric traits using diallel and factorial mating designs in bread wheat.	for studying the inheritance of metric traits, diallel cross and factorial mating designs are commonly used. since factorial mating design is less restrictive in crossing plans, the genetic information drawn from it was compared with that from a diallel cross. the comparison was made using graphical, genetic components and combining ability analyses for grain yield, grain weight and spike length in a field experiment of bread wheat (triticum aestivum l.). analyses were made on a nine parent dial ...	1985	24253828
intrachromosomal mapping of the nucleolar organiser region relative to three marker loci on chromosome 1b of wheat (triticum aestivum).	restriction enzyme digestion of the ribosomal rna genes of the nucleolar organisers of wheat has revealed fragment length polymorphisms for the nucleolar organiser on chromosome 1b and the nucleolar organiser on 6b. variation between genotypes for these regions has also been demonstrated. this variation has been exploited to determine the recombination frequency between the physically defined nucleolar organiser on 1b (designatednor1) and other markers; two loci,glu-b1 andgli-b1 which code for e ...	1985	24253819
the meiotic pairing of nine wheat chromosomes.	the meiotic identification of nine pairs of chromosomes at metaphase i of meiosis of triticum aestivum (b genome, 4a and 7a) has been achieved using a giemsa c-banding technique. as a result, the analysis of the pairing of each chromosome arm in disomic and monosomic intervarietal hybrids between 'chinese spring' and the spanish cultivar 'pané 247' could be carried out. differences in the chiasmata frequencies per chromosome arm cannot be explained on the basis of relative arm lengths only. poss ...	1984	24253711
a computer-assisted examination of the storage protein genetic variation in 841 accessions of triticum dicoccoides.	triticum turgidum l. var. dicoccoides (wild emmer) is an important genetic resource for increasing the protein content of common wheat (triticum aestivum l.). many studies have shown that the presence or absence of bands in sodium dodecyl sulfate polyacrylamide (sds-page) electrophoregrams of wheat storage proteins to be of a purely genetic character. a total protein extraction and sds-page technique was used to estimate the storage protein genetic variability among 841 accessions of wild emmer ...	1984	24253627
qualitative/quantitative strategy for the determination of glufosinate and metabolites in plants.	a simple method for the simultaneous determination of glufosinate and its metabolites in plants based on liquid chromatography-ultraviolet (lc-uv) absorption detection after derivatization with fluorenylmethoxycarbonyl chloride (fmoc-cl) of some analytes to facilitate separation is reported here. nonavailable standard metabolites were identified by lc-tof/mass spectrometry (ms), which also confirmed all target analytes. ultrasound-assisted extraction was used for sample preparation (power of 70 ...	2014	24253412
ethylene and reactive oxygen species are involved in root aerenchyma formation and adaptation of wheat seedlings to oxygen-deficient conditions.	exposing plants to hypoxic conditions greatly improves their anoxic stress tolerance by enhancing the activities of glycolysis and fermentation in roots. ethylene may also be involved in these adaptive responses because its synthesis is increased in roots under hypoxic conditions. here it is reported that pre-treatment of wheat seedlings with an ethylene precursor, 1-aminocyclopropanecarboxylic acid (acc), enhanced accumulation of ethylene in the roots of wheat seedlings, and enhanced their tole ...	2014	24253196
structural homology of endosperm high molecular weight glutenin subunits of common wheat (triticum aestivum l.).	several high molecular weight endosperm glutenin subunits, coded by genes located on chromosomes 1a, 1b and 1d of common wheat, triticum aestivum l. em. thell., were isolated from excised gel segments and subjected to amino acid analysis and peptide mapping; the latter was carried out following a limited digestion with trypsin, chymotrypsin or staphylococcus aureus - v8 protease. generally, all high molecular weight glutenins had a similar amino acid composition but several significant differenc ...	1985	24253121
cytogenetic analysis of forms produced by crossing hexaploid triticale with common wheat.	hexaploid triticales were crossed with common wheats, and the resultant froms were selected for either triticale (ad 213/5-80) or common wheat (lines 381/80, 391/80, 393/80). the cytogenetic analysis showed that all forms differ in their chromosome composition. triticale ad 213/5-80 and wheat line 381/80 were stable forms with 2n = 6x = 42. lines 391/80 and 393/80 were cytologically unstable. in triticale ad 213/5-80, a 2r (2d) chromosome substitution was found. each of the three wheat lines had ...	1985	24253064
meiotic pairing in hybrids between tetraploid triticale and related species: new elements concerning the chromosome constitution of tetraploid triticale.	two f5 strains of tetraploid triticale (2n= 4x=28), obtained from 6x triticalex2 rye progenies, were crossed with diploid and tetraploid rye, some durum and bread wheats, and various 8x and 6x triticale lines. meiosis in the different hybrid combinations was studied. the results showed that the haploid complement of these triticales consists of seven chromosomes from rye and seven chromosomes from wheat. high frequencies of pmcs showing trivalents were observed in hybrids involving the reference ...	1985	24253010
intrachromosomal mapping of crossability genes in wheat (triticum aestivum).	intrachromosomal mapping studies were used to locate the positions of the genes kr1 and kr2, which control the crossability of wheat with hordeum bulbosum, on chromosomes 5b and 5a, respectively. the location of kr1 was established using the telocentric mapping technique and found to be on the long arm of chromosome 5b, distal to the centromere with a mean recombination frequency of 44.8±3.28%. kr2 was located on the long arm of chromosome 5a by linkage with the major gene markers vrn1, controll ...	1985	24252927
factors affecting the induction of pollen plants of intergeneric hybrids of triticum aestivum x triticum-agropyron.	experimental results showed that the use of potato extract as a basic component of culture medium had a promoting effect on producing calli in anther culture of the intergeneric hybrids of triticum aestivum × triticum-agropyron (intermediate type). the induction frequencies of pollen callus on the potato-ii medium containing potato extract as the main component was much higher than that found on n6 and w5 media. the induction frequencies of pollen callus and green plantlets in four intergeneric ...	1985	24252924
identification of new aquaporin genes and single nucleotide polymorphism in bread wheat.	major facilitators of water movement through plant cell membranes include aquaporin proteins. wheat is among the largest and most important cereal crops worldwide; however, unlike other model plants such as rice, maize and arabidopsis, little has been reported on wheat major intrinsic proteins (mips). this study presents a comprehensive computational identification of 349 new wheat expressed sequence tags (ests), encoding 13 wheat aquaporin genes. identified aquaporins consist of 6 plasma membra ...	2013	24250219
the nadph-protochlorophyllide oxidoreductase is the major protein constituent of prolamellar bodies in wheat (triticum aestivum l.).	a fraction of highly purified prolamellar bodies was isolated from etioplasts of wheat (triticum aestivum l. cv. starke ii, weibull), as previously described by ryberg and sundqvist (1982, physiol. plant., 56, 125-132). studies on the protein composition revealed that only one major polypeptide of an apparent molecular weight of 36000 is present in the fraction of prolamellar bodies. this polypeptide was identified as the nadph-protochlorophyllide oxidoreductase. the highest specific activity of ...	1985	24249610
electrostatic surface properties of plasmalemma vesicles from oat and wheat roots. ion binding and screening investigated by 9-aminoacridine fluorescence.	right-side-out and sealed plasmalemma vesicles were isolated from roots of spring wheat (triticum aestivum l. cv. drabant) and oat (avena sativa l. cv. brighton) by two-phase partition in a medium containing sucrose (0.25 mol l(-1)). oat root plasmalemma vesicles were discovered to contain a strongly fluorescent compound with an emission maximum at 418 nm. the surface potential of the membranes was monitored by 9-aminoacridine fluorescence and the effect of protein concentration, mannitol versus ...	1985	24249604
specificity of an antibody to a subunit of high-molecular-weight storage protein from wheat seed and its reaction with other cereal storage proteins (prolamins).	an antiserum to subunit 2 from the high-molecular-weight (hmw) subunits of the glutenin fraction of triticum aestivum cv. highbury was shown to react with related subunits from other cultivars of wheat. the reaction was measured quantitatively by laser nephelometry in polyethylene glycol phosphate-buffered saline after dissolving the hmw fraction in 0.1 m acetic acid; urea used to dissolve the hmw prolamins inhibited the reaction, in some cases at the low concentration of 0.06 m. a study of the ...	1985	24249513
combining microtomy and confocal laser scanning microscopy for structural analyses of plant-fungus associations.	the serious problem of extended tissue thickness in the analysis of plant-fungus associations was overcome using a new method that combines physical and optical sectioning of the resin-embedded sample by microtomy and confocal microscopy. improved tissue infiltration of the fungal-specific, high molecular weight fluorescent probe wheat germ agglutinin conjugated to alexa fluor® 633 resulted in high fungus-specific fluorescence even in deeper tissue sections. if autofluorescence was insufficient, ...	2014	24249491
changes in the activities of chloroplast and cytosolic isoenzymes of glutamine synthetase during normal leaf growth and plastid development in wheat.	soluble protein extracts and chloroplasts from a serial sequence of transverse sections of a 7-d-old wheat leaf (triticum aestivum cv. maris huntsman) were used to study changes in the activity of glutamine synthetase (gs; ec 6.3.1.2) during cell and chloroplast development. glutamine synthetase activity increased more than 50-fold per cell from the base to the tip of the wheat leaf. two isoenzymes of gs were separated using fast protein liquid chromatography (fplc). glutamine synthetase localiz ...	1985	24249453
a freeze-etch study of the effects of extracellular freezing on cellular membranes of wheat.	seedlings of triticum aestivum l. cv. lennox were grown in different environments to obtain different hardiness. pieces of laminae and leaf bases were slowly cooled to sub-zero temperatures and the damage caused was assessed by an ion-leakage method. comparable pieces of tissue were slowly cooled to temperatures between 2° and-14°c and were then freeze-fixed and freeze-etched. membranes generally retained their lamellar structures indicated by the abundance of typical membrane fracture faces in ...	1985	24249403
wheat tissues freeze-etched during exposure to extracellular freezing: distribution of ice.	pieces excised from leaf bases and laminae of seedlings of triticum aestivum l. cv. lennox were slowly frozen, using a specially designed apparatus, to temperatures between 2° and 14° c. these treatments ranged from non-damaging to damaging, based on ion-leakage tests to be found in the accompanying report (pearce and willison 1985, planta 163, 304-316). the frozen tissue pieces were then freeze-fixed by rapidly cooling them, via melting freon, to liquid-nitrogen temperature. the tissue was subs ...	1985	24249402
induction of pistil-like structures in suspension-derived callus cultures of wheat (triticum aestivum).	in the following a method for the induction of pistil-like structures in wheat suspension cultures (triticum aestivum l.) is described. in young influorescences of plants, which were artificially infected with the wheat bunt fungi (tilletia controversa), organogenic calli with pistil-like structures could be induced on loblolly pine medium + 3 mg/l 3,6-dichloro-2-methoxy benzoic acid. the yield of these structures in calli from a five-month-old suspension culture was up to 100 per gram of callus ...	1987	24248846
suspension and protoplast culture of hexaploid wheat (triticum aestivum l.).	suspension cultures have been initiated from embryogenic callus of hexaploid wheat (triticum aestivum l.). most commonly, these "suspensions" are composed of callus-like clusters (up to 2 mm in diameter). two rapidly-growing lines (mbe6 and c82d) have been obtained, which consist of smaller aggregates of cytoplasmic cells, and these have been maintained for more than 4 years. these lines show very limited morphogenetic capacity and only a single plantlet has been regenerated, from line mbe6, aft ...	1987	24248442
stimulation of shoot regeneration in triticum aestivum and nicotiana plumbaginifolia viv. tissue cultures using the ethylene inhibitor agno3.	silver nitrate effectively promoted shoot regeneration in wheat (triticum aestivum l.) callus cultures derived from immature embryos. this effect could be observed in both weakly and strongly regenerating cultivars, and in using material from both field and greenhouse grown plants. the role of silver ions as an inhibitor of ethylene action was supported by a reversal of the inhibitory effects of 2,4-d and ethylene on morphogenesis in wheat callus cultures.enhancement of shoot regeneration by sil ...	1987	24248436
effect of illumination on the redox state of cytochrome c oxidase in wheat leaves in vivo.	the redox states of cytochrome a 3 in wheat leaves (triticum aestivum l.), in light and in the dark were monitored by its reaction with co, which resulted in the stimulation of the in vivo reduction of nitrate to nitrite by nitrate reductase under aerobic conditions. illumination of the leaves for 10 min markedly stimulated the steady state reduction of cytochrome a 3, probably associated with a decreased energization of the mitochondria in light. in the dark, during steady state respiration of ...	1986	24248241
molecular analysis of γ-gliadin gene families at the complex gli-1 locus of bread wheat (t. aestivum l.).	a cdna clone (ptag1436) carrying a complete coding sequence for a γ-gliadin polypeptide has been identified and sequenced. by hybridisation to size fractionated poly a(+) rna from wheat nullisomic-tetrasomic lines, homologous transcripts from the gli-a1, gli-b1 and gli-d1 loci were identified. these mrnas differed from those complementary to a low molecular weight (lmw) glutenin cdna clone. hybridization of ptag1436 to digested wheat dna produced a pattern of fragments unrelated to that obtained ...	1986	24248211
biochemical evidence of homoeology between triticum aestivum and agropyron intermedium chromosomes.	the alcohol dehydrogenase (adh), phosphoglucose mutase (pgm), glucosephosphate isomerase (gpi), glutamic oxaloacetic transaminase (got), malate dehydrogenase (mdh), leaf esterases (estl), leaf acid (acph) and endosperm alkaline (phe) phosphatases, leaf peroxidases (perl) zymogram phenotypes of triticum aestivum, agropyron intermedium, triticum aestivum - agropyron intermedium octoploids and six agropyron intermedium chromosome additions to triticum aestivum and two ditelocentric addition lines w ...	1986	24248208
genetics of yellow berry in wheat (triticum aestivum).	the inheritance of yellow berry, a grain disorder in durum and bread wheats, was studied in six intervarietal crosses in bread wheat. the trait was found to be controlled by either two or three dominant genes. monosomic analysis using 'chinese spring' monosomic series showed the presence of two major dominant genes on chromosomes 1a and 7a, and four modifiers on 4a, 4b, 6a and 6d, which influence the expression of yellow berry in bread wheat.	1986	24248030
genetic control of grain yield and its related traits in bread wheat.	genetic control of tiller number, grain number, grain weight, harvest index and grain yield in six generations, along with the biparentals, f3s, f2xparental progeny, and f2xf1 progeny were investigated in an intervarietal cross of bread wheat involving two highly competitive varieties, 'wl711' and 'hd 2009'. the performance of f1, b1, b2, f2, × p1, f2 × p2 and f2 × f1 progeny was midway between the parents involved with respect to all the evaluated characters. the biparental progeny excelled the ...	1986	24248028
wheat specific repetitive dna sequences - construction and characterization of four different genomic clones.	the construction and molecular analysis of four recombinant clones - pta1, pta2, pta7, and pta8 - is described. the four clones contain different highly repeated sequences of genomic dna from triticum aestivum variety 'chinese spring'. the wheat specificity has been determined by colony and dot blot hybridization in comparison with total rye dna (secale cereale variety 'petka'). the four clones with a variable degree of specificity were compared by sequence analysis after the recloning of wheat ...	1986	24247835
genes encoding α-amylase inhibitors are located in the short arms of chromosomes 3b, 3d and 6d of wheat (triticum aestivum l.).	three α-amylase inhibitors, designated inh. i, ii and iii have been purified from the 70% ethanol extract of hexaploid wheat (triticum aestivum l.) and characterized by amino acid analysis, n-terminal amino acid sequencing and enzyme inhibition tests. inhibitors i and iii have identical n-terminal sequences and inhibitory properties to those of the previously described 0.19/0.53 group of dimeric inhibitors. inhibitor ii has an n-terminal sequence which is identical to that of the previously desc ...	1986	24247781
genetic control of heading date and spikelet number in common wheat (t. aestivum l.) line 'noa'.	the phenology and build-up of spikelet number under 10 h day-length were studied in five wheat lines: the multispikelet line 'noa', the regular line 'mara', the f1 hybrid between them and monosomics 2d of 'mara' and of this hybrid (lacking the 2d chromosome of 'mara'). 'noa' had a longer spike development phase, a higher initial number of spikelet primordia and a slower rate of spikelet production than 'mara'. the f1 hybrid was similar to 'noa' in its high initial number of spikelets and to 'mar ...	1986	24247780
cytoplasmic effects on the tissue culture response of wheat (triticum aestivum) callus.	calli were initiated from immature embryos of eight lines of hexaploid wheat (triticum aestivum l. em. thell) with different cytoplasms, the euplasmic nuclear donor 'chinese spring' and seven alloplasmic lines derived from wild relative species of the genera triticum and aegilops. the calli were found to differ in their initial growth rates, their sensitivity to 2,4-d and their ability to organise shoot primordia, demonstrating that the cytoplasm can significantly affect the behaviour of tissues ...	1986	24247774
the effect of specific chromosome and cytoplasm substitutions on the tissue culture response of wheat (triticum aestivum) callus.	calli were initiated from immature embryos of four lines of hexaploid wheat (triticum aestivum l. em. thell), the euplasmic nuclear donor 'chinese spring', 'chinese spring' in which both 4b chromosomes were substituted by those of the variety 'cappelle-desprez' and two alloplasmic lines in which these nuclei were substituted into the cytoplasm of aegilops ovata. the calli were found to differ in their initia growth rates and their ability to organise shoot primordia and regenerate shoots. the 'c ...	1986	24247705
heritable somaclonal variation in gliadin proteins of wheat plants derived from immature embryo callus culture.	fertile r0 plants of the winter wheat line nd7532 (triticum aestivum l.) were regenerated from callus tissue after 60-190 days in culture. seeds produced from these self-pollinated plants were planted in the field. of the 5586 r1 plants, 32 differed for one or more agronomic traits from plants not passed through tissue culture process. gliadin electrophoregrams were prepared from bulk samples of r2 seed from these 32 plants. four of the 32 produced gliadin patterns different from controls, so 12 ...	1986	24247703
production, morphology, and cytogenetic analysis of elymus caninus (agropyron caninum) x triticum aestivum f1 hybrids and backcross-1 derivatives.	intergeneric hybrids were produced between common wheat, triticum aestivum (2n=6x=42, aabbdd) and wheatgrass, etymus caninus (agropyron caninum) (2n=4x=28, sshh) - the first successful report of this cross. reciprocal crosses and genotypes differed for percent seed set, seed development and f1 hybrid plant production. with e. caninus as the pollen parent, there was no hybrid seed set. in the reciprocal cross, seed set was 23.1-25.4% depending upon wheat genotype used. hybrid plants were produced ...	1986	24247612
genetical analysis of chromosome 5a of wheat and its influence on important agronomic characters.	chromosome 5a of bread wheat, triticum aestivum carries the major gene, vrnl, which is one of the main determinants of the winter/spring growth habit polymorphism in this species. genetical analysis of this chromosome has been carried out using single-chromosome recombinant lines to establish the pleiotropic effects of this locus and two other major genes, q determining ear morphology and bl determining the presence of awns, on important agronomic characters. the three major genes were located o ...	1985	24247464
the incorporation and characterization of powdery mildew resistance from aegilops longissima in common wheat (t. aestivum l.).	in the progeny of a hybrid between monotelosomic line 3b of 'chinese spring' wheat and 'chinese spring' - aegilops longissima ditelosomic addition line 'g' a cytologically stable strain was selected consisting of 20 wheat chromosome pairs, one pair of telosomic chromosome 3bl and one pair of telosomic longissima chromosome 'g'. inoculating 'chinese spring' - aegilops longissima addition and substitution lines with ten different powdery mildew isolates, partial resistance was observed. the infect ...	1985	24247463
the effect of wheat cytoplasm on the synthesis of wheat x rye hybrids.	four nuclear genotypes of triticum aestivum l., each substituted into the cytoplasm of t. timococcum kostoff, aegilops ventricosa tausch and t. timopheevi zhuk., were crossed with four homozygous secale cereale l. inbred lines to produce 48 alloplasmic octoploid primary triticales. crossability, embryo differentiation, amphihaploid plantlet recovery in vitro, and response to colchicine treatment were investigated. it was found that in general the effects of the wheat cytoplasm on the characteris ...	1985	24247454
organelle genome stability in anther-derived doubled haploids of wheat (triticum aestivum l., cv. 'moisson').	chloroplast and mitochondrial compartments of a parental line of wheat (triticum aestivum l., cv. 'moisson') and its anther-derived doubled haploid lines have been analyzed and compared on the basis of their dna restriction patterns. the results obtained show that no noticeable difference can be detected between doubled haploid lines and parental line at the level of ctdna and mtdna organization. it may be concluded that in vitro culture by itself does not systematically generate a cytoplasmic v ...	1985	24247401
nucleolus organizer competition in triticum aestivum - aegilops umbellulata chromosome addition lines.	the nucleolar organizer activity of wheat (triticum aestivum, aabbdd) and aegilops umbellulata (uu) chromosomes have been analyzed in the complete set of the chromosome addition lines by using a highly reproducible silver-staining procedure. chromosomes 1u and 5u produce the partial inactivation of wheat nucleolar organizer chromosomes 6b, 1b and 5d. the chromosomes d and g from ae. umbellulata, which are not sat-chromosomes, seem to specifically influence the activity of wheat nors. the predomi ...	1985	24247394
induction of recombination between rye chromosome 1rl and wheat chromosomes.	the ph1b mutant in bread wheat has been used to induce homoeologous pairing and recombination between chromosome arm 1rl of cereal rye and wheat chromosome/s. a figure of 2.87% was estimated for the maximal frequency of recombination between a rye glutelin locus tightly linked to the centromere and the heterochromatic telomere on the long arm of rye chromosome 1r in the progeny of ph1b homozygotes. this equates to a gametic recombination frequency of 1.44%. this is the first substantiated geneti ...	1985	24247384
relative efficiency of two mating systems and selection procedures for yield improvement in wheat (triticum aestivum l.).	the relative efficiences of the biparental mating systems and selfing series in connection with phenotypic and geno-phenotypic selection procedures were evaluated for yield improvement in a cross 'hp1102' x 'cpan 1681' of wheat. two selection cycles having a 4 per cent selection intensity for grain yield were carried out following both selection procedures under the two mating systems. following these selection procedures, a greater improvement for grain yield could be achieved with the biparent ...	1985	24247346
mutant alleles of photoperiod-1 in wheat (triticum aestivum l.) that confer a late flowering phenotype in long days.	flowering time in wheat and barley is known to be modified by mutations in the photoperiod-1 (ppd-1) gene. semi-dominant ppd-1a mutations conferring an early flowering phenotype are well documented in wheat but gene sequencing has also identified candidate loss of function mutations for ppd-a1 and ppd-d1. by analogy to the recessive ppd-h1 mutation in barley, loss of function mutations in wheat are predicted to delay flowering under long day conditions. to test this experimentally, introgression ...	2013	24244507
comparative genome analysis between agrostis stolonifera and members of the pooideae subfamily, including brachypodium distachyon.	creeping bentgrass (agrostis stolonifera, allotetraploid 2n = 4x = 28) is one of the major cool-season turfgrasses. it is widely used on golf courses due to its tolerance to low mowing and aggressive growth habit. in this study, we investigated genome relationships of creeping bentgrass relative to the triticeae (a consensus map of triticum aestivum, t. tauschii, hordeum vulgare, and h. spontaneum), oat, rice, and ryegrass maps using a common set of 229 est-rflp markers. the genome comparisons b ...	2013	24244501
genetic transformation of wheat via agrobacterium-mediated dna delivery.	the method described involves an initial incubation of wheat immature embryos in a liquid culture of agrobacterium tumefaciens. the agrobacterium strain is engineered to contain a binary vector with a gene of interest and a selectable marker gene placed between the t-dna borders; the t-dna is the region transferred to the plant cells, thus harnessing the bacterium's natural ability to deliver specific dna into host cells. following the initial inoculation with the agrobacterium, the embryos are ...	2014	24243208
genetic transformation of wheat via particle bombardment.	since its first invention in the late 1980s the particle gun has evolved from a basic gunpowder driven machine firing tungsten particles to one more refined which uses helium gas as the propellant to launch alternative heavy metal particles such as gold and silver. the simple principle is that dna-coated microscopic particles (microcarriers) are accelerated at high speed by helium gas within a vacuum and travel at such a velocity as to penetrate target cells. however, the process itself involves ...	2014	24243206
silencing of an aphid carboxylesterase gene by use of plant-mediated rnai impairs sitobion avenae tolerance of phoxim insecticides.	rna interference (rnai) describes the ability of double-stranded rna (dsrna) to inhibit homologous gene expression at the rna level. its specificity is sequence-based and depends on the sequence of one strand of the dsrna corresponding to part or all of a specific gene transcript. in this study we adopted plant-mediated rnai technology that targets sitobion avenae (s. avenae) to enable gene silencing in the aphid and to minimize handling of the insects during experiments. s. avenae was selected ...	2014	24242160
allelopathy of oats. ii. allelochemical effect ofl-tryptophan and its concentration in oat root exudates.	l-tryptophan caused growth inhibition of roots and hypocotyls (or coleoptiles) of cockscomb (amaranthus caudatus l.), lettuce (lactuca sativa l.), cress (lepidium sativum l.), timothy (phleum pratense l.), rice (oryza sativa l.), wheat (triticum aestivum l.), and oat (avena sativa l.), increasing the dose ofl-tryptophan increased the inhibition. the concentrations for 50% inhibition of the root growth were 0.14, 0.15, 0.21, 0.79, 0.95, 1.7, and 2.4 mm for cockscomb, cress, lettuce, timothy, rice ...	1994	24242057
callus formation and plantlet regeneration from protoplasts derived from suspension cultures of wheat (triticum aestivum l.).	protoplasts were isolated from anther-derived suspension cultures of commercial wheat (triticum aestivum l. cv. chris). the protoplasts were released enzymatically and isolated by centrifugation on a sucrose cushion. the isolated protoplasts were initially cultured in a liquid medium in the dark. numerous microcalli were produced under these conditions, some of which differentiated into globular embryos. upon transfer to a solid medium and exposure to 16h/8h light/dark cycle, the protocalli prol ...	1988	24241878
copy numbers of chloroplast and nuclear genomes are proportional in mature mesophyll cells of triticum and aegilops species.	the possibility of estimating the proportion of chloroplast dna (ctdna) and nuclear dna (ndna) in nucleic-acid extracts by selective digestion with the methylation-sensitive restriction enzyme psti, was tested using leaf extracts from spinacia oleracea and triticum aestivum. values of ctdna as percentage ndna were estimated to be 14.58%±0.56 (se) in s. oleracea leaves and 4.97%±0.36 (se) in t. aestivum leaves. these estimates agree well with those already reported for the same type of leaf mater ...	1986	24241861
monosomic analysis of heading date and spikelet number in the common wheat (triticum aestivum l.) multispikelet line 'noa'.	genetic analysis of heading date and spikelet number was carried out in the common wheat (triticum aestivum l.) multispikelet line 'noa', by using the monosomic series of the regular line 'mara'. 'noa's' high number of spikelets was found to be controlled by a recessive major gene on chromosome 2d; a slight reduction in spikelet number was induced by another recessive gene on 'noa's' 7a chromosome. 'noa's' late heading date was found to be controlled by two recessive genes, located on chromosome ...	1987	24241806
interaction between genes controlling a new group of glutenin subunits in bread wheat.	one-dimensional sodium dodecyl sulphate-polyacrylamide gel electrophoresis (sds-page) of reduced total protein extracts from the endosperm of hexaploid wheat revealed a new set of faintly-stained bands, having slower electrophoretic mobility than the high-molecular-weight (hmw) glutenin subunits. these new bands have been termed the e group of glutenin subunits. analysis of aneuploid stocks of chinese spring wheat has shown that three of the e bands, in order of increasing electrophoretic mobili ...	1987	24241800
the effect of the 4b chromosomes of hexaploid wheat on the growth and regeneration of callus cultures.	calli were initiated from immature embryos of nine lines of hexaploid wheat (triticum aestivum l. em. thell). these were the euploid lines chinese spring and cappelle-desprez, a line of chinese spring ditelocentric for the long arm of 4b, four substitution lines of chinese spring in which chromosome 4b has been replaced by its homologues from different wheat varieties and substituted into chinese spring and a substitution line of besostaya i 4b into cappelle-desprez. the calli from these lines w ...	1987	24241797
the effect of ph gene alleles on synaptonemal complex formation in triticum aestivum × t. kotschyi hybrids.	chromosome pairing at zygotene-pachytene was studied in triticum aestivum × t. kotschyi hybrids (2n=5x=35, genomic constitution abdc(u)s(v)) by electron microscopy of synaptonemal complexes in spread microsporocyte nuclei. hybrids carrying either the ph allele or the ph allele, which differ markedly in metaphase i pairing, are both capable of greater than 90% pachytene pairing, although pairing in the ph hybrids appeared slower or less synchronised. in both genotypes branched synaptonemal comple ...	1987	24241796
transfer of the 1bl/1rs wheat-rye-translocation from hexaploid bread wheat to tetraploid durum wheat.	the present study describes a cytological stable alien chromosome translocation in tetraploid durum wheat. by crossing the hexaploid 1bl/1rs wheat-rye translocation line "veery" to the tetraploid durum wheat cultivar "cando" it was possible to select a 28 chromosomic strain homozygous for the 1bl/1rs translocation. the disease resistance potential of the short arm of rye chromosome 1r, which has been widely introduced in many hexaploid bread wheat cultivars could be now also used for the improve ...	1987	24241794
genetic analysis of the anther-culture response of three spring wheat crosses.	anther-culture response was examined among three spring wheat (triticum aestivum l.) cultivars to evaluate the genetic component of response and to determine whether androgenetic performance could be improved by selection. the three lines, the three possible f1's among the three lines, their f2's, and the backcrosses to the parents were evaluated for callus production and regeneration capacity. significant variation was observed among the generations of the three crosses for callus formation. ge ...	1987	24241670
the addition of dasypyrum villosum (l.) candargy chromosomes to durum wheat (triticum durum desf.).	six monosomic addition lines were produced in which different dasypyrum villosum (l.) candargy chromosomes were added to the chromosome complement of triticum durum desf. cv. 'creso'. each added alien chromosome was found to have a specific effect on plant morphology and fertility. transmission rate varied widely (from 7.5 to 27.7%) among the six univalent chromosomes. different monotelosomic addition plants derived by a relatively high frequency of chromosome misdivision were isolated. the addi ...	1987	24241669
identification and chromosomal locations of aconitase gene loci in triticeae species.	two systems of monomeric aconitase (aco) isozymes, designated aco-1 and aco-2, were identified in triticum aestivum and in five diploid triticeae species. the gene loci aco-a1, aco-b1, and aco-d1 were located in t. aestivum cv. 'chinese spring' chromosome arms 6aq, 6bq, and 6dq, respectively, and the gene loci aco-a2, aco-b2, and aco-d2 in 5 aq, 5 bq, and 5dq, respectively. aco-1 gene loci were also identified in 6eβ of elytrigia elongata, 6hl of hordeum vulgare cv. 'betzes', 6rl of secale cerea ...	1987	24241574
genetic variability for heat shock proteins in common wheat.	the response of the common wheat line 'chinese spring' to heat shocks of different time lengths was studied by the two-dimensional (2d) electrophoresis of denatured proteins. after a heat shock of 5 h, 33 heat shock proteins (hsps) accumulated in an amount sufficient to be revealed by silver stain. two other wheat lines ('moisson' and 'selkirk') were then submitted to a heat shock of 5 h, and the responses of the 3 lines were compared: of a total of 35 hsps, 13 (37.1%) were quantitatively or qua ...	1987	24241566
substitution analysis of callus induction and plant regeneration from anther culture in wheat (triticum aestivum l.).	the genetic determination of callus induction, total plant regeneration and green plant regeneration from anther culture were studied using a "chinese spring"/"cheyenne" substitution series. all the three characteristics were found to be polygenically determined, but their inheritance was independent from one another. the 7a and 18 chromosomes had a considerable effect on callus induction. in the case of total plant regeneration the most influential chromosome "as the 3a while the 2d chromosome ...	1988	24241549
detoxification of n-(phosphonoacetyl)-l-aspartate by carrot cells in suspension culture.	in bacterial and mammalian cells, n-(phosphonoacetyl)-l-aspartate (pala) suppresses growth by strongly inhibiting aspartate transcarbamoylase (atcase; ec 2.1.3.2), a key enzyme of the pyrimidine biosynthetic pathway. at a concentration that would suppress growth in mammalian or bacterial cells, and that is nearly a million-fold greater than the inhibition constant (k i ) for atcase in carrot (daucus carota) seedling extracts, pala does not suppress growth of carrot cells in suspension culture. t ...	1985	24241524