Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
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| subunit topology of the rhodococcus proteasome. | the 20s proteasome, isolated from the nocardioform actinomycete rhodococcus erythropolis strain ni86/21, is built from two alpha-type and two beta-type subunits. in order to probe the subunit topology, we have set up an expression system which allows coexpression of the genes encoding the alpha- and beta-subunits in all possible combinations. the four respective constructs obtained yielded fully assembled and proteolytically active proteasomes. biochemical, kinetic and electron microscopy analys ... | 1997 | 9000518 |
| a phylogenetic analysis of aerobic polychlorinated biphenyl-degrading bacteria. | several bacterial isolates were characterized based on their abilities to degrade specific polychlorinated biphenyls (pcbs) and their 16s rrna gene sequences. the members of one group of bacteria consisting of alcaligenes species, including the pcb-degrading bacterium alcaligenes eutrophus h850, had strong abilities to degrade a broad range of pcbs but not the di-para-chlorine-substituted congeners. the members of another group, which included the pcb-degrading bacterium originally classified as ... | 1997 | 8995824 |
| a proposal to reclassify nocardia pinensis blackall et al. as skermania piniformis gen. nov., comb. nov. | the type strain of nocardia pinensis was the subject of chemotaxonomic and 16s ribosomal dna sequencing studies. the resultant nucleotide sequence was aligned with the sequences of representatives of the genera corynebacterium, dietzia, gordona, mycobacterium, nocardia, rhodococcus, and tsukamurella, and phylogenetic trees were generated by using the fitch-margoliash, maximum-parsimony, maximum-likelihood, and neighbor-joining methods. it was evident from the phylogenetic analyses that n. pinens ... | 1997 | 8995814 |
| [the degradation of plasticizers by rhodococcus erythropolis 40f]. | a rhodococcus erythropolis 40 phi strain isolated from the soil utilizes 0.1-2% v/v dibutylphthalate (dbp) and di-(2-ethylhexyl)-phthalate (dehp) as a single carbon source. the strain metabolizes dbp and dehp via the following steps: diester --> phthalate --> benzoate --> p-hydroxybenzoate --> 3,4-dihydroxybvenzoate --> 3-ketoadipinate. | 1996 | 8991593 |
| cloning, analysis, and overexpression of the gene encoding isobutylamine n-hydroxylase from the valanimycin producer, streptomyces viridifaciens. | the flavoprotein isobutylamine n-hydroxylase (ibah) catalyzes the oxidation of isobutylamine to isobutylhydroxylamine, a key step in the biosynthesis of the azoxy antibiotic valanimycin. by using oligonucleotide primers designed from peptide sequence information derived from native ibah, a fragment of the gene (vlmh) encoding ibah was amplified by pcr from a genomic library of the valanimycin-producing organism, streptomyces viridifaciens mg456-hf10. the gene fragment was then employed as a prob ... | 1997 | 8990292 |
| characterization of catechol catabolic genes from rhodococcus erythropolis 1cp. | the biochemical characterization of the muconate and the chloromuconate cycloisomerases of the chlorophenol-utilizing rhodococcus erythropolis strain 1cp previously indicated that efficient chloromuconate conversion among the gram-positive bacteria might have evolved independently of that among gram-negative bacteria. based on sequences of the n terminus and of tryptic peptides of the muconate cycloisomerase, a fragment of the corresponding gene has now been amplified and used as a probe for the ... | 1997 | 8990288 |
| a novel transporter involved in cobalt uptake. | cobalt is an essential component of a low molecular-mass nitrile hydratase (l-nhase) from rhodococcus rhodochrous j1. we have found a new gene, nhlf, in the dna region sandwiched between nhlba encoding l-nhase and amda encoding amidase, which are involved in the degradation of nitriles. the product of nhlf, nhlf, shows a significant sequence similarity with those of hoxn from alcaligenes eutrophus, hupn from bradyrhizobium japonicum, nixa from helicobacter pylori, and ureh from bacillus sp., whi ... | 1997 | 8990157 |
| resonance raman evidence that photodissociation of nitric oxide from the non-heme iron center activates nitrile hydratase from rhodococcus sp. n-771. | nitrile hydratase (nhase) from rhodococcus sp. n-771, which contains a non-heme iron center in the catalytic site, has been known to be activated by light illumination. recently, endogenous nitric oxide (no) was found in this enzyme by ftir spectroscopy [noguchi et al. (1995) febs lett. 358, 9-12]. in order to directly detect the bonding between no and the iron atom and the reaction of no upon photoactivation, resonance raman spectra of the nhase were measured with 413 nm excitation at 85 k. two ... | 1996 | 8988015 |
| nitrile hydratase and its application to industrial production of acrylamide. | nitrile hydratase (nhase) was discovered in our laboratory. this enzyme was purified and characterized from various microorganisms. nhases are roughly classified into two groups according to the metal involved: fe-type and co-type. nhases are expected to have great potential as catalysts in organic chemical processing because they can convert nitriles to the corresponding higher-value amides under mild conditions. we have used microbial enzymes for the production of useful compounds: nhase has b ... | 1996 | 8987584 |
| succinoyl trehalose lipid induced differentiation of human monocytoid leukemic cell line u937 into monocyte-macrophages. | a novel type of succinoyl trehalose lipid (stl-1) prepared from n-hexadecane-culture of rhodococcus erythropolis sd-74 markedly inhibited the growth of a human monocytoid leukemic cell line, u937, and induced its morphological alteration along a monocyte-macrophage lineage. stl-1 markedly increased differention-associated characteristics in macrophage, such as nitroblue tetrazolium reducing ability, appearance of fc receptor, phagocytic activities in u937. furthermore, u937 cells, which were act ... | 1995 | 8987500 |
| identification of rhodococcus equi using the polymerase chain reaction. | two regions in the gene coding for 16s rrna in rhodococcus equi were selected as species-specific primer sequences for the polymerase chain reaction (pcr). pcr using these primers was tested against 10 strains of r. equi (including the type strain) and gave positive results for all but was negative for all other tested species of rhodococcus; representatives of the most closely related genera and a number of other bacterial species. this method could therefore be used to identify this species wh ... | 1996 | 8987445 |
| cytologic features of pulmonary malakoplakia related to rhodococcus equi in an immunocompromised host. | cytologic features are described in bronchial brushings of a large cavitary lung mass from an immunosuppressed patient who had undergone liver transplantation. scattered histiocytes with abundant eosinophilic, vacuolated cytoplasm were noted in a background of bronchial cells. within approximately one fourth of the histiocytes, targetoid intracytoplasmic inclusions were present, consistent with michaelis-gutmann bodies. they were diffusely positive by histo-chemical staining with von kossa, gomo ... | 1996 | 8982589 |
| amide metabolism: a putative abc transporter in rhodococcus sp. r312. | the dna sequence has been determined upstream of the amie structural gene in the amidase operon of rhodococcus sp. r312 and a new orf (amis2) identified. the amis2 gene encodes a potential 206 amino acid (aa) protein containing a high proportion of hydrophobic residues. the amis2 protein possesses high homology to the orfp3, amis and urei gene products from the mycobacterium smegmatis (ms) acetamidase operon, pseudomonas aeruginosa (pa) amidase operon and helicobacter pylori (hp) urease operon, ... | 1996 | 8982091 |
| characterization of three distinct extradiol dioxygenases involved in mineralization of dibenzofuran by terrabacter sp. strain dpo360. | the dibenzofuran-degrading bacterial strain dpo360 represents a new species of the genus terrabacter together with the previously described dibenzofuran-mineralizing bacterial strain dpo1361 (k.-h. engesser, v. strubel, k. christoglou, p. fischer, and h. g. rast, fems microbiol. lett. 65:205-210, 1989; v. strubel, ph.d. thesis, university of stuttgart, stuttgart, germany, 1991; v. strubel, h. g. rast, w. fietz, h.-j. knackmuss, and k.-h. engesser, fems microbiol. lett. 58:233-238, 1989). two 2,3 ... | 1997 | 8981980 |
| monooxygenase-like sequence of a rhodococcus equi gene conferring increased resistance to rifampin by inactivating this antibiotic. | a dna clone from rhodococcus equi conferring low-level rifampin resistance through the ability to inactivate this antibiotic via its decomposition was identified. the iri (inactivation of rifampin) gene consisted of an open reading frame of 1,437 bp encoding a 479-amino-acid sequence strongly resembling those of monooxygenases acting upon phenolic compounds or involved in polyketide antibiotic synthesis. when expressed in escherichia coli, the gene conferred resistance to a > 50-micrograms/ml co ... | 1997 | 8980786 |
| radiological findings in nine aids patients with rhodococcus equi pneumonia. | rhodococcus equi (r. equi) infections have been incidentally reported as a cause of pulmonary infection in severely immunocompromised hosts, including aids patients. our purpose is to describe the radiological findings in nine aids patients with r. equi pneumonia assessed by bronchoalveolar lavage (bal), biopsies, cultures of sputum, and hemocultures. all patients were examined by chest radiographs and contrast-medium-enhanced chest ct. dense pulmonary consolidations with or without cavitations ... | 1996 | 8972317 |
| community-acquired bacteremia in human immunodeficiency virus-infected children in harare, zimbabwe. | hiv infection is common in mothers and their children in zimbabwe, and hiv-infected children are particularly susceptible to bacterial infections. there is little information on the etiology and outcome of hiv-related bacteremia in african children. | 1996 | 8970218 |
| studies on the isopropylbenzene 2,3-dioxygenase and the 3-isopropylcatechol 2,3-dioxygenase genes encoded by the linear plasmid of rhodococcus erythropolis bd2. | the enzymes responsible for the degradation of isopropylbenzene (ipb) and co-oxidation of trichloroethene (tce) by rhodococcus erythropolis bd2 are encoded by the linear plasmid pbd2. fragments containing ipb catabolic genes were cloned from pbd2 and the nucleotide sequence was determined. by means of database searches and expression of the cloned genes in recombinant strains, we identified five clustered genes, ipba1a2a3a4c, which encode the three components of the ipb 2,3-dioxygenase system, r ... | 1996 | 8969521 |
| routine use of pcr-restriction fragment length polymorphism analysis for identification of mycobacteria growing in liquid media. | a pcr-restriction fragment length polymorphism (pcr-rflp) procedure capable of rapidly identifying 28 species of clinically encountered mycobacteria was evaluated for use in the routine identification of acid-fast isolates growing in bactec 12b and 13a liquid media. pcr-rflp identified 100 of 103 acid-fast isolates recovered from 610 patient specimens submitted for culture during the study. the three isolates unidentifiable by pcr-rflp produced restriction patterns not included in the pcr-rflp a ... | 1997 | 8968884 |
| isolation of rhodococcus rhodochrous ncimb13064 derivatives with new biodegradative abilities. | rhodococcus rhodochrous ncimb13064 can dehalogenate and utilise a number of halogenated aliphatic compounds as sole carbon and energy source. mutants of ncimb13064 can be easily isolated with an enlarged range of 1-chloroalkane utilising ability. dehalogenation of 1-chlorononane, 1-chlorodecane and short-chain 1-chloroalkanes (c3-c8) is encoded by the same plasmid prtl1. however, a different genetic element(s) is required for the dehalogenation of 3-chloropropionic acid. two derivatives (p200 an ... | 1996 | 8961560 |
| the use of a novel recombinant heroin esterase in the development of an illicit drugs biosensor. | 1996 | 8958079 | |
| differentiation of rhodococcus species by ribotyping. | the ribosomal rna gene restriction patterns (ribotyping) was investigated with respect to the characterization of rhodococcus species and r. rhodochrous strains. chromosomal dna was prepared, digested with bamh i, blotted, and hybridized with acetylaminofluorene-labelled 16 + 23s rrna from e. coli. the type strains of seven rhodococcus species studied gave different hybridization patterns in each case. all strains tested were clearly distinguishable by ribotyping. patterns contained two to eight ... | 1996 | 8956489 |
| rhodococcus equi infection in hiv-positive subjects: a retrospective analysis of 24 cases. | rhodococcus equi causes a rare infection in immunocompromised hosts. we describe 24 cases of infection in patients with aids-related complex (arc)/acquired immunodeficiency syndrome (aids). pneumonia was always the first manifestation of r. equi infection, but extrapulmonary involvement was also observed. the main sources of bacteria were sputum, bronchial washings and blood. the strains isolated were mainly susceptible to erythromycin, vancomycin, teicoplanin, rifampicin, imipenem and aminoglyc ... | 1996 | 8953675 |
| [generalized rhodococcosis of fatal outcome in a patient with aids]. | 1996 | 8949612 | |
| a chemotaxonomic study of the lipoglycans of rhodococcus rhodnii n445 (ncimb 11279). | rhodococcus rhodnii n445 was investigated for the presence of macroamphiphilic lipoglycan. purification of a hot phenol-water extract by hydrophobic interaction chromatography allowed the resolution of three lipoglycan fractions. the two main preparations contained lipoglycans with carbohydrate compositions consistent with the presence of lipoarabinomannan and lipomannan, whilst the minor fraction appeared to contain a mixture of these two lipoglycans. the fatty acid composition of the lipoglyca ... | 1996 | 8946691 |
| rhodococcus equi causing bacteraemia and pneumonia in a pulmonary transplant patient. | 1996 | 8945718 | |
| rhodococcus equi in capd-associated peritonitis treated with azithromycin. | 1996 | 8941608 | |
| genetic analysis of the dsz promoter and associated regulatory regions of rhodococcus erythropolis igts8. | the dsz gene cluster of rhodococcus erythropolis igts8 comprises three genes, dsza, dszb, and dszc, whose products are involved in the conversion of dibenzothiophene (dbt) to 2-hydroxybiphenyl and sulfite. this organism can use dbt as the sole sulfur source but not as a carbon source. dsz activity is repressed by methionine, cysteine, casamino acids, and sulfate but not by dbt or dimethyl sulfoxide. we cloned 385 bp of the dna immediately 5' to dsza in front of the reporter gene lacz of escheric ... | 1996 | 8932295 |
| failure of hyperimmune plasma to prevent pneumonia caused by rhodococcus equi in foals. | a trial was conducted on a thoroughbred stud to determine whether or not the administration of anti-rhodococcus equi hyperimmune plasma would reduce the prevalence of r equi pneumonia (rattles) in foals born in the 1992 horse breeding season. hyperimmune plasma was administered to 34 foals; another 57 foals were untreated. there was no significant difference in the number of transfused foals developing r equi pneumonia compared with the untreated foals. the time required for recovery from pneumo ... | 1995 | 8929188 |
| relationship between haemagglutination and hela-cell adherence of rhodococcus equi. | in this study, 45 rhodococcus equi isolates from animals and humans were identified by the api coryne system, and serologically with monospecific antisera against capsular types 1-7, with serotypes 1 and 2 predominating. regardless of serotype, 14 out of 31 isolates from animals and one of 14 isolates from humans expressed 15-17-kd virulence-associated proteins. in hexadecane adherence studies, serotype 2 isolates generally displayed hydrophobic surfaces. in addition, isolates of serotype 1 gene ... | 1996 | 8928574 |
| simultaneous utilization of pyridine and fructose by rhodococcus opacus ufz b 408 without an external nitrogen source. | a bacterium classified as rhodococcus opacus, which is able to use pyridine (a potentially growth-inhibiting substrate) as its sole source of carbon, energy and nitrogen, was isolated. in a carbon-limited chemostat culture, the kinetics was determined for growth on both pyridine and a mixture of pyridine and fructose (9 mm/22.15 mm). with growth on pyridine, stable steady states were achieved up to dilution rates of about 0.1 h-1. a further increase in the dilution rate resulted in the progressi ... | 1996 | 8920194 |
| serum antibody responses of foals to virulence-associated 15- to 17-kilodalton antigens of rhodococcus equi. | humoral immune responses in 16 foals to virulence-associated 15- to 17-kda antigens of rhodococcus equi were studied during the first fourteen weeks of life on two horse-breeding farms with a persistent incidence of r. equi infection. serum antibody levels specific for 15- to 17-kda antigens were measured by enzyme-linked immunosorbent assay and western immunoblotting. immunoglobulin g (igg) antibodies specific to 15- to 17-kda antigens were detected by all the foals. r. equi was found in the fe ... | 1996 | 8914251 |
| the beta-ketoadipate pathway and the biology of self-identity. | the beta-ketoadipate pathway is a chromosomally encoded convergent pathway for aromatic compound degradation that is widely distributed in soil bacteria and fungi. one branch converts protocatechuate, derived from phenolic compounds including p-cresol, 4-hydroxybenzoate and numerous lignin monomers, to beta-ketoadipate. the other branch converts catechol, generated from various aromatic hydrocarbons, amino aromatics, and lignin monomers, also to beta-ketoadipate. two additional steps accomplish ... | 1996 | 8905091 |
| relationship between induction of macrophage chemotactic factors and formation of granulomas caused by mycoloyl glycolipids from rhodococcus ruber (nocardia rubra). | mycoloyl glycolipids cause granulomas in the lungs, liver, and spleen of mice, but the mechanism is not fully understood. to understand the role of macrophage chemotactic factors (mcfs) in granuloma formation, we prepared various mycoloyl glycolipids with different carbohydrate moieties: trehalose dimycolate (tdm), glucose mycolate (gm), mannose mycolate (mm), and fructose mycolate (fm) from rhodococcus ruber, and examined the relationship between their mcf induction in peritoneal macrophages an ... | 1996 | 8902634 |
| allogeneic bone marrow transplantation can restore cd4+ t-lymphocyte count and immune function in idiopathic cd4+ t-lymphocytopenia. | cd4+ t-lymphocytopenia in the absence of hiv infection is a heterogeneous disorder of unknown cause. here we report a patient with idiopathic cd4+ t-lymphocytopenia, presenting with an opportunistic rhodococcus equi infection. when aplastic anemia developed subsequently, allogeneic bone marrow transplantation was performed. complete restoration of immune function was observed. we conclude that allogeneic bone marrow transplantation presents a potentially curative therapy for cd4+ t-lymphocytopen ... | 1996 | 8899203 |
| extra-pulmonary rhodococcus equi in a thoroughbred foal. | 1996 | 8896881 | |
| use of a virulence-associated protein based enzyme-linked immunosorbent assay for rhodococcus equi serology in horses. | an enzyme-linked immunosorbent assay (elisa) was developed against rhodococcus equi using triton x-114 detergent extracted whole cell material, in which the virulence associated protein (vapa) predominated. enzymelinked immunosorbent assay titres corresponded to antibody reacting with vapa on western blots. there was considerable variation in antibody titres of nonimmunised mares and in the time when the colostrally derived antibody of their foals had declined to low or undetectable titres. in g ... | 1996 | 8894530 |
| rhodococcal pneumonia: humoral versus cell-mediated immunity. | 1996 | 8894528 | |
| the incidence and consequences of failure of passive transfer of immunity on a thoroughbred breeding farm. | circulating igg concentration was determined between 12 and 24 hours after birth for 323 foals born on a thoroughbred breeding farm over 3 consecutive years. the incidence of failure of passive transfer (fpt) of maternal immunoglobulins (foal circulating igg concentration < 8 g/l) was found to be 9.6%. foals born late in the season (october to december) were found to be at increased risk for the development of fpt. the degree of assistance required at parturition and the presence of a peripartur ... | 1996 | 8893988 |
| expression of virulence-associated antigens of rhodococcus equi is regulated by temperature and ph. | we recently reported that there are two different virulence-associated antigens correlated with virulence levels in rhodococcus equi isolates from aids patients: virulent r. equi that kills mice with 10(6) cells expresses 15- to 17-kda antigens and intermediately virulent r. equi that kills mice with 10(7) cells expresses a 20-kda antigen. environmental parameters were evaluated for their effects on the expression of these virulence-associated antigens in virulent r. equi strains by immunoblotti ... | 1996 | 8887354 |
| poststenotic cavitating pneumonia due to rhodococcus equi in hiv infection. | 1996 | 8882676 | |
| in vitro activity of the trinem sanfetrinem (gv104326) against gram-positive organisms. | the in vitro activity of the trinem sanfetrinem (formerly gv104326) (gv) was compared with that of vancomycin, ampicillin, and/or nafcillin against 287 gram-positive bacteria, including methicillin-resistant staphylococcus aureus and multiresistant enterococci, by the agar and microbroth dilution methods. gv demonstrated 2 to 16 times more activity than ampicillin and nafcillin against the majority of these organisms. the mic range of gv was 16 to 64 micrograms/ml for 19 enterococcus faecium str ... | 1996 | 8878596 |
| serologic responses to rhodococcus equi in individuals with and without human immunodeficiency virus infection. | thirty healthy blood donors, 15 workers from horse-breeding farms, 69 human immunodeficiency virus (hiv)-negative persons at risk for hiv infection, 125 hiv-infected subjects without rhodococcus equi infection, and nine hiv-infected patients with rhodococcus equi pneumonia were evaluated in order to detect serum antibodies to rhodococcus equi precipitate-soluble antigen by an enzyme immunoassay (eia). whereas eia values for healthy donors, horse farm workers, individuals at risk for hiv infectio ... | 1996 | 8874077 |
| different rifampicin inactivation mechanisms in nocardia and related taxa. | mycolic acid-containing bacteria inactivate rifampicin in a variety of ways such as glucosylation, ribosylation, phosphorylation and decolorization. these inactivations were found to be a species-specific phenomena in nocardia and related taxa. gordona, tsukamurella and fast-growing mycobacterium modified rifampicin by ribosylation of the 23-oh group of the antibiotic. such ribosylation was not observed in rhodococcus and corynebacterium, but phosphorylation of the 21-oh group of rifampicin was ... | 1996 | 8871521 |
| genetic analysis of clavibacter toxicus, the agent of annual ryegrass toxicity. | multilocus enzyme electrophoresis was used to examine the relatedness of 52 isolates of clavibacter toxicus, the agent of annual ryegrass toxicity. these included 37 western australian (wa) field isolates sampled in 3 distinct locations over a 2-year period, and 15 isolates sampled from 6 different host plant species in 3 states in australia over approximately 8 years. seventeen reference strains for the related genera curtobacterium, rhodococcus and arthrobacter were examined for comparison. th ... | 1996 | 8870638 |
| growth of rhodococcus s1 on anthracene. | three slow-growing bacteria were isolated from a mixed culture enriched for growth on anthracene, using creosote-contaminated soil as the inoculum. organisms were shown to use anthracene by the production of a clear zone around the colony after a mineral salts agar plate was sprayed with anthracene. all three bacteria were nonmotile, nonsporulating, gram-positive rods and stained acid-fast. physiological and biochemical tests, gc content, and cell wall lipid patterns of whole cell methanolysates ... | 1996 | 8868237 |
| empyema with malakoplakic-like lesions by rhodococcus equi as a presentation of hiv infection. | rhodococcus equi (corynebacterium equi) is an aerobic actinomycetes, well described as a cause of pulmonary infection in different animals as horses, pigs and cows. this pathogen has a coccobacillar aspect and a variable acid-fast stain in tissues. rare cases of human infection by rhodococcus species were described, the majority by rhodococcus equi, especially in patients with immunodeficiency syndrome (aids) in advanced stages of the disease. usually the diagnosis of infections by rhodococcus s ... | 1995 | 8868180 |
| genetic differences between nitrate-negative and nitrate-positive c. pseudotuberculosis strains using restriction fragment length polymorphisms. | corynebacterium pseudotuberculosis has been classified into two biotypes according to ability to breakdown nitrate (biberstein et al., 1971). restriction enzyme analysis (rea) has shown to reflect this differentiation, but numerous bands generated by this technique make interpretation difficult (songer et al., 1988). restriction fragment length polymorphism's (rflp's) has become an accepted genetic tool and was used in this study to determine if differences in nitrate reduction and other phenoty ... | 1996 | 8861638 |
| transcriptional regulation of the rhodococcus rhodochrous j1 nita gene encoding a nitrilase. | the 1.4-kb downstream region from a nitrilase gene (nita) of an actinomycete rhodococcus rhodochrous j1, which is industrially in use, was found to be required for the isovaleronitrile-dependent induction of nitrilase synthesis in experiments using a rhodococcus-escherichia coli shuttle vector pk4 in a rhodococcus strain. sequence analysis of the 1.4-kb region revealed the existence of an open reading frame (nitr) of 957 bp, which would encode a protein with a molecular mass of 35,100. deletion ... | 1996 | 8855219 |
| cholesterol oxidase from rhodococcus equi is likely the major factor involved in the cooperative lytic process (camp reaction) with listeria monocytogenes. | the camp reaction between listeria monocytogenes and rhodococcus equi was studied by a diffusion assay. listeria monocytogenes displayed identical cooperative haemolytic effect with supernatant cultures of r. equi or with commercial cholesterol oxidase (cox). this result, even with enzymes of different sources (commercial cox is obtained from pseudomonas spp.) suggests that this enzyme secreted by r. equi has a crucial role in the synergistic haemolytic (camp) reaction with l. monocytogenes. the ... | 1996 | 8852354 |
| isolation of virulent and intermediately virulent rhodococcus equi from soil and sand on parks and yards in japan. | rhodococcus equi is an emerging opportunistic pathogen of human immunodeficiency virus-infected patients. however, little is known about the distribution of virulent and intermediately virulent r. equi in human environment. in the present study, r. equi was isolated from 173 of 234 (73.9%) samples collected from soil and sand on 115 parks and 49 yards in japan. the numbers of r. equi from soil and sand ranged from 2.5 x 10(1) to 1.2 x 10(5) per gram of sample. none of 1,294 isolates from those s ... | 1996 | 8844605 |
| an identification scheme for rapidly and aerobically growing gram-positive rods. | an identification scheme for aerobically growing gram-positive rods (genera actinomyces, arcanobacterium, aureobacterium, bacillus, brevibacterium, cellulomonas, corynebacterium, dermabacter, erysipelothrix, gardnerella, lactobacillus, listeria, microbacterium, oerskovia, propionibacterium, rhodococcus, rothia, turicella, as well as unnamed cdc groups, clostridium tertium, and mycobacterium fortuitum/chelonae) is presented. it is derived from the hollis-weaver scheme and uses catalase, oxidative ... | 1996 | 8837385 |
| photoreactive nitrile hydratase: the photoreaction site is located on the alpha subunit. | nitrile hydratase (nhase) from rhodococcus sp. n-771 exists in active and inactive forms. the inactive nhase is immediately activated by light irradiation and changes to the active form. to characterize the photoreactive center, the inactive nhase was denatured by 6 m urea, and two kinds of subunits (alpha and beta) were separated and purified by anion-exchange chromatography. in a manner similar to the native nhase, the isolated alpha subunit showed two absorption peaks at 280 and 370 nm, which ... | 1996 | 8830032 |
| equine herpesvirus type 2 (ehv-2) as a predisposing factor for rhodococcus equi pneumonia in foals: prevention of the bifactorial disease with ehv-2 immunostimulating complexes. | equine herpesvirus type 2 (ehv-2), a member of the gammaherpesvirinae subfamily, was studied in a two-phase respiratory disease complex of young foals as a predisposing factor for the secondary bacterial invasion of lungs with rhodococcus equi (r. equi). foals were immunized against ehv-2 on a farm where r. equi pneumonia regularly occurred during the last years. the immunizations were performed by using a subunit vaccine which selectively presents envelope glycoproteins of ehv-2 in a multimeric ... | 1996 | 8828122 |
| attempts to find phenotypic markers of the virulence plasmid of rhodococcus equi. | four isolates of rhodococcus equi, from pneumonic foals, and containing the 85 kb virulence plasmid, a porcine isolate containing an 80 kb plasmid, and their plasmid cured derivatives, were examined for 239 phenotypic properties in an attempt to find characters other than the virulence-associated protein (vapa) which might be encoded by the virulence plasmid in organisms grown at 37 degrees c. tests chosen included those which have previously given variable results for r. equi isolates, since su ... | 1996 | 8825990 |
| rhodococcus equi: an emerging opportunistic pathogen. | rhodococcus equi is emerging as a cause of pneumonia in immunocompromised people, especially those with aids. like mycobacteria, r. equi is phagocytosed by alveolar macrophages and replicates within them. recent work is beginning to elucidate the cell and molecular biology of this opportunistic pathogen and the host immune response to it. | 1996 | 8824792 |
| gene overexpression, purification, and identification of a desulfurization enzyme from rhodococcus sp. strain igts8 as a sulfide/sulfoxide monooxygenase. | the oxidation of dibenzothiophene to dibenzothiophene sulfone has been linked to the enzyme encoded by the sox/dszc gene from rhodococcus sp. strain igts8 (s. a. denome, c. oldfield, l. j. nash, and k. d. young, j. bacteriol. 176:6707-6717, 1994; c. s. piddington, b. r. kovacevich, and j. rambosek, appl. environ. microbiol. 61:468-475, 1995). however, this enzyme has not been characterized, and the type of its catalytic activity remains unclassified. in this work, the sox/dszc gene was overexpre ... | 1996 | 8824615 |
| bacteremia due to gordona sputi in an immunocompromised patient. | we report a case of bacteremia due to a strain identified as gordona sputi in a patient with metastatic melanoma. the origin of infection remains unknown, but extensive cutaneous lesions due to interleukin-2 treatment may have been the portal of entry. the isolate was related to g. sputi on the basis of its biochemical and genomic properties but exhibited some differences from the type strain. | 1996 | 8818912 |
| identification of intermediately virulent rhodococcus equi isolates from pigs. | we recently reported the existence of rhodococcus equi isolates with at least three virulence levels, isolated from aids patients: virulent r. equi having 15- to 17-kda antigens that kills mice with 10(6) cells, intermediately virulent r. equi having a 20-kda antigen that kills mice with 10(7) cells, and avirulent r. equi that does not kill mice with 10(8) cells or more (s. takai, y. imai, n. fukunaga, y. uchida, k. kamisawa, y. sasaki, s. tsubaki, and t. sekizaki, j. infect. dis. 172:1306-1311, ... | 1996 | 8815079 |
| 2-d crystallization of the rhodococcus 20s proteasome | the 2d crystallization method using a liquid-liquid interface has been applied to the rhodococcus 20s proteasome. two types of ordered arrays were obtained, both large enough for high-resolution analysis. the first one is a hexagonal close-packed array, whereas the second one has fourfold symmetry. by image analysis based on a real space correlation averaging technique, the close-packed array was found to be hexagonally packed but the molecules had complete rotational freedom. the fourfold array ... | 1996 | 8813003 |
| role of cd4+, cd8+ and double negative t-cells in the protection of scid/beige mice against respiratory challenge with rhodococcus equi. | to evaluate the contributions of t-lymphocyte subsets in pulmonary immunity against rhodococcus equi, c.b-17 scid/beige mice were adoptively transferred with splenic lymphocytes from congenic balb/c mice previously infected with r. equi. spleen cells were enriched for either cd4+ or cd8+ populations before inoculation, flow cytometry showed that each enriched population contained less than 0.5% cross contamination. groups of adoptively transferred scid/beige mice were sacrificed 6 and 13 d after ... | 1996 | 8809381 |
| the microbiological fate of polycyclic aromatic hydrocarbons: carbon and oxygen balances for bacterial degradation of model compounds. | a series of pure bacterial strains belonging mainly to the rhodococcus and pseudomonas genera were grown on one of the following polycyclic aromatic hydrocarbons (pah) supplied as sole carbon and energy source; naphthalene, fluorene, phenanthrene, anthracene, fluoranthene and pyrene. in each case, a quantitative evaluation of the carbon repartition of the pah degraded into co2, biomass and water-soluble metabolites was carried out. in addition, the kinetics of oxygen consumption and of water-sol ... | 1996 | 8785039 |
| taxonomic note: a proposal for reviewing the interpretation of the camp reaction between listeria monocytogenes and rhodococcus equi. | the discrepancies between the current description of the camp test between listeria monocytogenes and rhodococcus equi in the latest edition of bergey's manual of determinative bacteriology (l. monocytogenes is described as camp test negative with r. equi) and routine findings (positive reactions are usually described in many laboratories) make it advisable to review the current interpretation of the camp test to avoid confusion among people working in microbiological laboratories. overall, 98.4 ... | 1996 | 8782698 |
| biodegradation of 4-nitroanisole by two rhodococcus spp. | two rhodococcus strains, r. opacus strain as2 and r. erythropolis strain as3, that were able to use 4-nitroanisole as the sole source of carbon and energy, were isolated from environmental samples. the first step of the degradation involved the o-demethylation of 4-nitroanisole to 4-nitrophenol which accumulated transiently in the medium during growth. oxygen uptake experiments indicated the transformation of 4-nitrophenol to 4-nitrocatechol and 1,2,4-trihydroxybenzene prior to ring cleavage and ... | 1996 | 8782395 |
| reciprocal action of interferon-gamma and interleukin-4 promotes granulomatous inflammation induced by rhodococcus aurantiacus in mice. | an intravenous injection of rhodococcus aurantiacus to mice causes granulomatous inflammation dependent on endogenous interferon-gamma (ifn-gamma). the present study examined the role of endogenous interleukin-4 (il-4) on granulomatous inflammation. endogenous il-4 in the spleen extracts was not detected during the phase of granuloma formation by enzyme-linked immunosorbent assay (elisa). however, il-4 protein level was elevated during the phase of granuloma regression. il-4 mrna expression in t ... | 1996 | 8774356 |
| genetic tools in pathogenic nocardioform actinomycetes. | nocardioform actinomycetes are gram-positive bacteria with high g+c content. several species of mycobacterium, corynebacterium, nocardia and rhodococcus are important human or animal pathogens. transposon mutagenesis and homologous recombination are powerful genetic tools used for the identification of pathogenicity determinants. transposable elements with potential application to mutagenize pathogenic nocardioform actinomycetes are described. homologous recombination experiments which have been ... | 1996 | 8767712 |
| [rhodococcus equi pulmonary abscess in hiv infection]. | re is a well-known gram positive bacillus which is usually pathogenic in animals. disease in humans is rare, but incidence has clearly increased with the advent of aids. in humans, re predominantly infect people with impaired cellular immunity so that it is considered an opportunistic agent. its must common manifestation in immuno-compromised patients is a slowly progressive pneumonia which may cavitate. even with early diagnostic and optimal and prolonged antibiotic therapy, the mortality of re ... | 1996 | 8763102 |
| atrazine chlorohydrolase from pseudomonas sp. strain adp: gene sequence, enzyme purification, and protein characterization. | pseudomonas sp. strain adp metabolizes atrazine to carbon dioxide and ammonia via the intermediate hydroxyatrazine. the genetic potential to produce hydroxyatrazine was previously attributed to a 1.9-kb avai dna fragment from strain adp (m. l. de souza, l. p. wackett, k. l. boundy-mills, r. t. mandelbaum, and m. j. sadowsky, appl. environ. microbiol. 61:3373-3378, 1995). in this study, sequence analysis of the 1.9-kb avai fragment indicated that a single open reading frame, atza, encoded an acti ... | 1996 | 8759853 |
| x-ray spectroscopy of nitrile hydratase at ph 7 and 9. | the iron k-edge x-ray absorption spectrum of rhodococcus sp. r312 (formerly brevibacterium sp. r312) nitrile hydratase in frozen solutions at ph 7 and 9 has been analyzed to determine details of the iron coordination. exafs analysis implies two or three sulfur ligands per iron and overall six coordination; together with previous epr and endor results, this implies an n3s2o ligation sphere. the bond lengths from exafs analysis [rav(fe-s) = 2.21 a at ph 7.3; rav(fe-n/o) = 1.99 a] support cis coord ... | 1996 | 8756470 |
| resonance raman spectroscopy of nitrile hydratase, a novel iron-sulfur enzyme. | resonance raman spectra of rhodococcus sp. r312 (formerly brevibacterium sp. r312) nitrile hydratase, a novel non-heme iron enzyme, have a large number of peaks in the 300-500 cm-1 region; observation of shifts in these peaks after labeling with 34s shows that they arise from cysteine coordinated to the ferric ion in the protein. the rich raman spectra result from coupling of the fe-s stretch with cysteine side chain deformation modes; the observation of 15n isotope shifts in most of these peaks ... | 1996 | 8756469 |
| [current concepts and implications for future research on rhodococcus equi]. | 1996 | 8752374 | |
| assessment of the biodegradation potential of psychrotrophic microorganisms. | bioremediation of polluted temperate and cold temperature environments may require the activity of psychrotrophic bacteria, because their low temperature growth range parallels the ambient temperatures encountered in these environments. in the present study, 135 psychrotrophic microorganisms isolated from a variety of ecosystems in canada were examined for their ability to mineralize 14c-labelled toluene, naphthalene, dodecane, hexadecane, 2-chlorobiphenyl, and pentachlorophenol. a number of the ... | 1996 | 8742353 |
| immunohistochemical detection of virulence-associated antigens of rhodococcus equi in pulmonary lesions of foals. | rhodococcus equi was isolated from the lungs of six foals with bronchopneumonia. all isolates expressed 15-17-kd antigens by immunoblot analysis and contained a virulence-associated plasmid of 85 or 90 kb. immunohistochemically, r. equi from all pulmonary lesions showed the expression of 15-17-kd antigens mainly in the phagocytic cells. the specific monoclonal antibody to 15-17-kd antigens of r. equi (mab 10g5) may be an aid in the diagnosis of r. equi-induced pneumonia. | 1996 | 8740709 |
| pulmonary malakoplakia in acquired immunodeficiency syndrome: an ultrastructural study of morphogenesis of michaelis-gutmann bodies. | malakoplakia is an unusual inflammatory reaction to a variety of infections, characterized by the accumulation of macrophages containing the target-like calcospherites, the michaelis-gutmann body (mgb). we report three patients with acquired immunodeficiency syndrome with pulmonary malakoplakia associated with rhodococcus equi infection; two patients were diagnosed at autopsy and one by examination of a transbronchial biopsy specimen. all three patients had pulmonary bacterial cultures and light ... | 1996 | 8733761 |
| molecular aspects of pesticide degradation by microorganisms. | microorganisms are able to degrade a large variety of compounds, including pesticides under laboratory conditions. however, methods have yet to be developed to decontaminate the environment from residues of pesticides. pesticidal degradative genes in microbes have been found to be located on plasmids, transposons, and/or on chromosomes. recent studies have provided clues to the evolution of degradative pathways and the organization of catabolic genes, thus making it much easier to develop geneti ... | 1996 | 8729958 |
| rhodococcus equi mastoiditis in a patient with aids. | 1996 | 8729214 | |
| cavitary pulmonary lesions in patients infected with human immunodeficiency virus. | the differential diagnosis of cavitary pulmonary lesions in individuals infected with human immunodeficiency virus (hiv) is broad, especially in patients with advanced disease. in patients with pneumocystis carinii pneumonia, cavitation is an uncommon manifestation of a common disease. it is unusual in patients with pulmonary cryptococcosis, coccidioidomycosis, and histoplasmosis but occurs frequently in patients with invasive pulmonary aspergillosis. in patients with pulmonary tuberculosis, cav ... | 1996 | 8729207 |
| rhodococcus equi infection in hiv-infected patients. | to report clinical and microbiological features and response to treatment in hiv patients with rhodococcus equi infection. | 1996 | 8728038 |
| identification of an alternative 2,3-dihydroxybiphenyl 1,2-dioxygenase in rhodococcus sp. strain rha1 and cloning of the gene. | gram-positive rhodococcus sp. strain rha1 possesses strong polychlorinated biphenyl-degrading capabilities. an rha1 bphc gene mutant, strain rdc1, had been previously constructed (e. masai, a. yamada, j. m. healy, t. hatta, k. kimbara, m. fukuda, and k. yano, appl. environ. microbiol. 61:2079-2085, 1995). an alternative 2,3-dihydroxybiphenyl 1,2-dioxygenase (2,3-dhbd), designated etbc, was identified in rdc1 cells grown on ethylbenzene. etbc contained the broadest substrate specificity of any me ... | 1996 | 8702287 |
| [rhodococcus equi pneumonia in patients with hiv infection: report of 2 cases and review of the literature]. | pneumonia by rhodococcus equi is infrequent and is associated with patients with important immunosuppression. to date 66 cases of pneumonia by rhodococcus equi in patients with hiv infection have been published. the diagnosis, problems in determining diagnosis and treatment are discussed. | 1996 | 8695688 |
| rhodococcus equi infection in 3 aids patients. | three cases of aids complicated by rhodococcus equi infection are reported. at least one of the patients acquired his rhodococcus infection in africa. despite the fact that the r. equi strains were susceptible to tetracycline, erythromycin, amikacin, co-trimoxazole, rifampicin and vancomycin, these antibiotics were clinically not successful. a clinical improvement was observed in only one patient during teicoplanin and imipenem-cilastatin treatment. multicentre clinical trials are needed to dete ... | 1996 | 8693866 |
| blastogenic response of lymphocytes from foals infected with rhodococcus equi. | the blastogenic response of lymphocytes from 16 newborn foals naturally infected with rhodococcus equi was investigated, in order to evaluate the relationship between r. equi infection and depressed host response. naturally infected foals showed evidence of r. equi infection at 5-6 weeks of age, as determined by clinical, haematological, bacteriological and serological methods. the blastogenic response of lymphocytes against phytohaemagglutinin was significantly depressed (stimulation index < 1. ... | 1996 | 8693847 |
| location and sequence analysis of a 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase-encoding gene (bpdf) of the biphenyl/polychlorinated biphenyl degradation pathway in rhodococcus sp. m5. | the 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate (hopd) hydrolase-encoding gene (bpdf) in the biphenyl (bp)/polychlorinated biphenyl (pcb)-degrading bacterium, rhodococcus sp. m5 (m5), was found to be located within a 4.5-kb hindiii-bamhi genomic dna that was 5.4 kb downstream from the bpdc1c2bade gene cluster. the deduced amino acid (aa) sequence of bpdf revealed that the hydrolase contains 297 aa (32679 da) that was verified by expression in the escherichia coli t7 rna polymerase/promoter system. ... | 1996 | 8675030 |
| a novel gene cluster including the rhodococcus rhodochrous j1 nhlba genes encoding a low molecular mass nitrile hydratase (l-nhase) induced by its reaction product. | the 3.5 kilobases (kb) of the 5'-upstream region from nhlba encoding a cobalt-containing low molecular mass nitrile hydratase (l-nhase) from rhodococcus rhodochrous j1 was found to be required for the amide-dependent expression of nhlba in experiments using a rhodococcus transformation system. sequence analysis of the 3.5-kb fragment revealed the presence of two open reading frames (nhld and nhlc) in this fragment. nhld has similarity to regulators merr, cadc, and arsr. nhlc has similarity to th ... | 1996 | 8662959 |
| formation of intracytoplasmic lipid inclusions by rhodococcus opacus strain pd630. | an oleaginous hydrocarbon-degrading rhodococcus opacus strain (pd630) was isolated from a soil sample. the cells were able to grow on a variety of substrates and to produce large amounts of three different types of intracellular inclusions during growth on alkanes, phenylalkanes, or non-hydrocarbon substrates. electron microscopy revealed large numbers of electron-transparent inclusions with a sphere-like structure. in addition, electron-dense inclusions representing polyphosphate and electron-t ... | 1996 | 8661931 |
| identification of a rhodococcus gene cluster encoding a homolog of the 17-kda antigen of brucella and a putative regulatory protein of the asnc-lrp family. | by sequence analysis, a gene encoding a homolog of the 17-kda protein antigen of the gram-negative pathogen brucella abortus (hemmen et al., clin diagn lab immunol 2: 263, 1995) was identified in the nocardioform actinomycete rhodococcus sp. ni86/21. database searching also revealed a partial human cdna sequence for a putative eukaryotic homolog of this presumptive brucella-specific protein. these proteins display a low but significant level of similarity with lumazine synthases involved in bact ... | 1996 | 8661681 |
| location of the non-heme iron center on the alpha subunit of photoreactive nitrile hydratase from rhodococcus sp. n-771. | nitrile hydratase (nhase) from rhodococcus sp. n-771, which possesses a non-heme iron center binding nitric oxide (no), is activated by light irradiation. to localize the iron center in the protein, we quantified fe atoms and performed ftir measurements of the isolated alpha and beta subunits. the native nhase and the isolated alpha subunit contained about 1.0 and 0.8 mol fe per mol protein, respectively, whereas the beta subunit contained only a trace of fe. an no stretching band was observed a ... | 1996 | 8660325 |
| the use of recombinant dna technology in the design of a highly specific heroin sensor. | 1996 | 8659922 | |
| purification and properties of an amidase from rhodococcus erythropolis mp50 which enantioselectively hydrolyzes 2-arylpropionamides. | an enantioselective amidase from rhodococcus erythropolis mp50 was purified to homogeneity. the enzyme has a molecular weight of about 480,000 and is composed of identical subunits with molecular weights of about 61,000. the nh2-terminal amino acid sequence was significantly different from previously published sequences of bacterial amidases. the purified amidase hydrolyzed a wide range of aliphatic and aromatic amides, the highest enzyme activities were found with amides carrying hydrophobic re ... | 1996 | 8655547 |
| rhodococcus peritonitis in continuous ambulatory peritoneal dialysis. | 1996 | 8649638 | |
| [rhodococcus equi lung infection in aids patients. a report of 4 cases]. | 1996 | 8643865 | |
| an unusual case of untreated chronic lymphocytic leukemia patient presenting with rhodococcus equi bacteriemia. | 1996 | 8638642 | |
| physical, biochemical, and immunological characterization of a thermostable amidase from klebsiella pneumoniae nctr 1. | an amidase capable of degrading acrylamide and aliphatic amides was purified to apparent homogeneity from klebsiella pneumoniae nctr 1. the enzyme is a monomer with an apparent molecular weight of 62,000. the ph and temperature optima of the enzyme were 7.0 and 65 degrees c, respectively. the purified amidase contained 11 5,5-dithiobis(2-nitrobenzoate) (dtnb)-titratable sulfhydryl (sh) groups. in the native enzyme 1.0 sh group readily reacted with dtnb with no detectable loss of activity. titrat ... | 1996 | 8636044 |
| characterization of the gene cluster of high-molecular-mass nitrile hydratase (h-nhase) induced by its reaction product in rhodococcus rhodochrous j1. | the 4.6-kb region 5'-upstream from the gene encoding a cobalt-containing and amide-induced high molecular mass-nitrile hydratase (h-nhase) from rhodococcus rhodochrous j1 was found to be required for the expression of the h-nhase gene with a host-vector system in a rhodococcus strain. sequence analysis has revealed that there are at least five open reading frames (h-orf1 approximately 5) in addition to h-nhase alpha- and beta-subunit genes. deletion of h-orf1 and h-orf2 resulted in decrease of n ... | 1996 | 8633053 |
| rhodococcus equi infection after liver transplantation. | 1996 | 8623173 | |
| distribution of thiols in microorganisms: mycothiol is a major thiol in most actinomycetes. | mycothiol [2-(n-acetylcysteinyl)amido-2-deoxy-alpha-d-glucopyranosyl- (1-->1)-myo-inositol] (msh) has recently been identified as a major thiol in a number of actinomycetes (s. sakuda, z.-y. zhou, and y. yamada, biosci. biotech. biochem. 58:1347-1348, 1994; h. s. c. spies and d. j. steenkamp, eur. j. biochem. 224:203-213, 1994; and g. l. newton, c. a. bewley, t. j. dwyer, r. horn, y. aharonowitz, g. cohen, j. davies, d. j. faulkner, and r. c. fahey, eur. j. biochem. 230:821-825, 1995). since thi ... | 1996 | 8606174 |
| transfer of a cd4+ th1 cell line to nude mice effects clearance of rhodococcus equi from the lung. | rhodococcus equi, and intracellular respiratory pathogen, causes sever e granulomatous pneumonia in humans with aids and in young horses. pulmonary clearance of r. equi requires functional cd4+ t cells and gamma interferon (ifn-gamma) expression from bronchial lymph node cells. the purpose of this study was to investigate whether r. equi-specific cd4+ th1 cells could effect clearance of r. equi from the lung. adoptive transfer of a clearance of r. equi from the lungs. in contrast, mice transfuse ... | 1996 | 8606068 |
| rapid growth and increased biomass yield of mycobacterium farcinogenes and some related taxa in broth and agar media. | a broth medium containing yeast extract (4 g/l), glucose (15 g/l), magnesium sulphate (0.5 g/l), tri-sodium citrate (1.5 g/l), potassium sulphate (0.5 g/l), ammonium ferric citrate (trace) and buffered with potassium di-hydrogen phosphate (5 g/l) was formulated and found to support a luxuriant growth of mycobacterium farcinogenes strains that was superior to the conventional media used before. similar results were obtained with m. senegalense strains and with representatives of the genera gordon ... | 1995 | 8594852 |
| characterization of the expression of the thcb gene, coding for a pesticide-degrading cytochrome p-450 in rhodococcus strains. | a cytochrome p-450 system in rhodococcus strains, encoded by thcb, thcc, and thcd, participates in the degradation of thiocarbamates and several other pesticides. the regulation of the system was investigated by fusing a truncated lacz in frame to thcb, the structural gene for the cytochrome p-450 monooxygenase. analysis of the thcb-lacz fusion showed that the expression of thcb was 10-fold higher in the presence of the herbicide eptc (s-ethyl dipropylthiocarbamate). similar enhancement of the t ... | 1996 | 8593046 |
| effect of penicillin g on the electroporation of rhodococcus rhodochrous cf222. | suitable conditions for the introduction of bacteriophage dna into cells of rhodococcus rhodochrous cf222 by electroporation were established, and penicillin g was found to enhance the transfection frequency. when conditions optimal for the parental strain were applied to its colony-morphological mutants, different transfection frequencies were observed. penicillin g enhanced the transfection frequency of smooth and mucoidal mutants but not of rough mutants. | 1996 | 8588890 |