Publications

study on dissimilatory reduction of sulphates.	a new strain of sulphate reducing bacteria was isolated from swampy forest soil. after 120 h reduction, sulphate conversion attained 100%, the molar ratio of the consumed lactate and reduced sulphate amounted to 2:1. this confirms the reduction mechanism proposed by senez (1951).	1979	87119
[staining of sulfate-reducing bacteria with hexacyanoferrat-compounds (cultural and cytochemical evidence of fe) (author's transl)].	hexacyanoferrat-compounds are suitable 1. to indicate the valence of fe-ions in cultures of desulfuricants (desulfovibrio spec., desulfotomaculum) in fluid and solid medium before and after inoculation; 2. stain directly the sulfate-reducing bacteria in presence of fe-ions and give a colour-reaction of those parts of the cell containing fe-compounds, e.g. fes. therefore they act as a cytochemical indicator system. the chemical base of the reactions are as follows: (1) 4hs + so42 leads to h2s + 2 ...	1978	77605
localized intracellular polyphosphate formation by desulfovibrio gigas.	the dissimilatory sulphate-reducing bacterium desulfovibrio gigas, frequently sub-cultured, often contained spherical granules which stained metachromatically with some basic dyes. the granules were examined in situ by transmission electron microscopy of whole organisms and thin sections. the granules were isolated from broken bacteria as a water-insoluble, non-crystalline, white material containing magnesium, phosphorus and organic carbon, but devoid of sulphur and nitrogen. the molar ratio of ...	1975	50405
[hexacyanoferrate-compounds as indicator substances for the efficacy of antimicrobial agents to desulfuricants (author's transl)].	hexacyanoferrate-compounds are suitable to indicate the efficacy of antimicrobial substances to sulfate-reducing bacteria. they act as a cytochemical indicator system. (stübner, 9), e. g. k3feiii(cn)6 showes fes formation before any visible blackening of the medium. by using hexacyanoferrate compounds it is easier to determine the efficacy of antimicrobial agents. in addition a microscopical control is recommended to detect damaged or avital bacteria in the limiting concentrations of the agents.	1979	44792
hydrogen bonding of flavoprotein. i. effect of hydrogen bonding on electronic spectra of flavoprotein.	the effect of hydrogen bonding on the transition energy and the oscillator strength of the isoalloxazine nucleus of flavins was studied by the molecular orbital method. among the possible hydrogen bondings examined, characteristic spectral shifts were found for the hydrogen bondings at n(1) and n(5) of the nucleus. the hydrogen bonding at n(1) resulted in the shift of the first absorption band towards blue and that of the second one towards red. on the other hand, the hydrogen bonding at n(5) re ...	1978	28779
growth yields and growth rates of desulfovibrio vulgaris (marburg) growing on hydrogen plus sulfate and hydrogen plus thiosulfate as the sole energy sources.		1978	28099
studies on the reaction of imidazole with cytochrome c3 from desulfovibrio vulgaris.		1978	26395
effect of pressure upon the fluorescence of various flavodoxins.	the effects of hydrostatic pressure in the range of 10(-3) to 11 kbar on the fluorescence of flavodoxins from peptostreptococcus elsdenii, desulfovibrio vulgaris, azotobacter vinelandii, and clostridium mp were investigated. the first three flavoproteins showed under high pressure enhancements of flavin fluorescence of over 50 times resulting from the release of flavin mononucleotide from the protein complex. the clostridial flavodoxin showed a very much smaller fluorescence change. at ph 7.5 th ...	1977	20943
the tricarboxylic and acid pathway in desulfovibrio.	strains of two species of desulfovibrio were examined for enzymes of the tricarboxylic acid cycle and related pathways. pyruvate carboxylase (ec6.4.1.1) is present, and alpha-ketoglutarate is formed via the tricarboxylic acids. glutamate, but not succinyl-coa, arises from alpha-ketoglutarate. a pathway exists from pyruvate by malic enzyme (ec 1.1.1.39) activity to malate, then fumarate and succinate, again with no evidence of succinyl-coa formation. the enzymes concerned with metabolism of these ...	1977	18274
variations in the spectrum of desulfoviridin from desulfovibrio gigas.	desulfoviridin preparations from d. gigas showed variations in the position of the absorption maximum the beta-peak) in the 580-nm region of the specturm. on treatment with na2s2o4 a preparation with a beta-peak at 585 nm was affected rapidly, the 585-nm peak shifting to the 596-nm region; this was partially reversed by k3fe(cn)6. treatment of the original preparation with k3fe(cn)6 resulted in a shift of the beta-peak to 582-583 nm. desulfoviridins with beta-peaks from 580 to 583 nm were not ra ...	1976	14608
non-heme iron proteins. the amino acid sequence of rubredoxin from desulfovibrio vulgaris.	a non-heme iron protein, rubredoxin has been isolated from the sulfate-reducing bacterium, desulfovibrio vulgaris, strain hildenborough. the complete amino acid sequence has been established. the 52 amino acid residues of the protein were aligned with the aid of tryptic and chymotryptic peptides and of a fragment produced by cleavage of the asn-gly bond (22-23) by hydroxylamine. the sequence of the first 30 residues of the molecule was determined using an automatic sequenator, after removal of t ...	1976	7308
resonance raman scattering from hemoproteins. effects of ligands upon the raman spectra of various c-type cytochromes.	resonance raman spectra were measured for various c-type cytochromes (mammalian cytochrome c, bacterial cytochrome c3, algal photosynthetic cytochrome f, and alkylated cytochrome c) and a b-type cytochrome (cytochrome b5) in their reduced and oxidized states. (1) for ferrous alkylated cytochrome c, a raman line sensitive to the replacement of an axial ligand of the heme iron uas found around 1540 cm=1. this ligand-sensitive raman line indicated the transition from acidic (1545 cm-1) to alkaline ...	1975	2584