Publications

TitleAbstractYear
Filter
PMID(sorted descending)
Filter
capd-related peritonitis due to salmonella enteritidis in a patient with sle.patients with systemic lupus erythematosus are prone to various infections, often associated with high mortality. salmonella species are a rare cause of continuous ambulatory peritoneal dialysis (capd) peritonitis, but patients with lupus are susceptible to infection caused by salmonella species. a 47-year-old woman who had systemic lupus erythematosus with end-stage renal disease and was undergoing capd presented with fever, watery diarrhea, abdominal pain, and turbid dialysate effluent. the ef ...200516112034
[molecular analysis of salmonella enteritidis by pulsed-field gel electrophoresis].to study the relationship between different strains of salmonella enteritidis, a molecular epidemiologic analysis method-pulsed-field gel electrophoresis was established and salmonella enteritidis isolated from food samples were tested.200516111047
evaluation of a french elisa for the detection of salmonella enteritidis and salmonella typhimurium in flocks of laying and breeding hens.in france, the regular and compulsory detection of salmonella enteritidis (se) and salmonella typhimurium (st) in flocks of breeding and laying hens is based on bacteriological examination of environmental swabs and faeces samples. the aim of this study was to compare this bacteriological examination with a serological method (elisa) developed in our laboratory. this elisa was first evaluated by use of artificially infected hens. during these experimental infection studies, several groups of hen ...200516102859
profile of toll-like receptor expressions and induction of nitric oxide synthesis by toll-like receptor agonists in chicken monocytes.toll-like receptors (tlrs) play a major role in the innate immune system for initial recognition of microbial pathogens and pathogen associated components. nitric oxide (no) is generated in immune cells in response to microbial stimulation and is involved in pathogenesis and control of infection. we used rt-pcr analysis to examine the tlr expression profile on chicken monocytes and demonstrated these cells express chicken tlr2, 3, 4, 6, and 7. tlr5 was not detected by the tr-pcr. we also investi ...200616098593
longitudinal monitoring of two commercial layer flocks and their environments for salmonella enterica serovar enteritidis and other salmonellae.between august 20, 2001, and september 17, 2002, 1429 samples including drag swabs, egg belt or egg rollout swabs, fan-blade swabs, rodent organ and intestinal pools, beetle (alphitobius diaperinus) pools, housefly (musca domestica) pools, chicken organ and intestinal pools, and egg pools were obtained for salmonella culture from two flocks from two different commercial layer ranches. the two ranches were purposefully selected for the study based on their previous status of salmonella enteritidi ...200516094821
a genome scan for quantitative trait loci affecting the salmonella carrier-state in the chicken.selection for increased resistance to salmonella colonisation and excretion could reduce the risk of foodborne salmonella infection. in order to identify potential loci affecting resistance, differences in resistance were identified between the n and 6(1) inbred lines and two qtl research performed. in an f2 cross, the animals were inoculated at one week of age with salmonella enteritidis and cloacal swabs were carried out 4 and 5 wk post inoculation (thereafter called csw4f2 and csw4f2) and cae ...200516093014
correlation of phenotype with the genotype of egg-contaminating salmonella enterica serovar enteritidis.the genotype of salmonella enterica serovar enteritidis was correlated with the phenotype using dna-dna microarray hybridization, ribotyping, and phenotype microarray analysis to compare three strains that differed in colony morphology and phage type. no dna hybridization differences were found between two phage type 13a (pt13a) strains that varied in biofilm formation; however, the ribotype patterns were different. both pt13a strains had dna sequences similar to that of bacteriophage fels2, whe ...200516085829
bacteriophages may bias outcome of bacterial enrichment cultures.enrichment cultures are widely used for the isolation of bacteria in clinical, biotechnological, and environmental studies. however, competition, relative growth rates, or inhibitory effects may alter the outcome of enrichment cultures, causing the phenomenon known as enrichment bias. bacteriophages are a major component in many microbial systems, and it abounds in natural settings. this abundance means that bacteriophages are likely to be present in many laboratory enrichment cultures. our hypo ...200516085813
risk factors for contamination of ready-to-eat street-vended poultry dishes in dakar, senegal.our objective was to investigate the salmonella and campylobacter contamination of traditional ready-to-eat street-vended poultry dishes and to assess the association of some restaurant characteristics and cooking practices with the contamination of these meals. one hundred and forty-eight street-restaurants were studied from january 2003 to april 2004 in dakar. a questionnaire was submitted to the managers, and samples of ready-to-eat poultry dishes were taken. salmonella spp. was isolated in 2 ...200516083818
inactivation of staphylococcus aureus and salmonella enteritidis in tryptic soy broth and caviar samples by high pressure processing.we studied the action of high pressure processing on the inactivation of two foodborne pathogens, staphylococcus aureus atcc 6538 and salmonella enteritidis atcc 13076, suspended in a culture medium and inoculated into caviar samples. the baroresistance of the two pathogens in a tryptic soy broth suspension at a concentration of 10(8)-10(9) colony-forming units/ml was tested for continuous and cycled pressurization in the 150- to 550-mpa range and for 15-min treatments at room temperature. the i ...200516082468
[abscess of the spleen].splenic abscess is a rare disease but with increasing frequency. the authors present 9 patients with splenic abscess treated at the institute of digestive system diseases, clinical centre of serbia, in a period from january 1, 1986 to may 15, 2004. splenic abscess was the complication of septic endocarditis in 4, trauma in 2, dental infection in 1, while in 2 cases it was the complication of chemotherapy in myeloproliferative disorders. all 9 patients had fever, 7 - abdominal pain, 4 - left shou ...200516053175
antibacterial activity of cerein 8a, a bacteriocin-like peptide produced by bacillus cereus.the mode of action of cerein 8a, a bacteriocin produced by the soil bacterium bacillus cereus 8a, was investigated. the effect of cerein 8a was tested against listeria monocytogenes and a bactericidal effect at 400 arbitrary units (au)/ml was observed. in addition, cerein 8a was bactericidal against bacillus cereus at 200 au/ml, and inhibited the growth of escherichia coli and salmonella enteritidis. stronger inhibition of these gram-negative bacteria was achieved when the chelating agent edta w ...200516052461
use of a specific bacteriophage treatment to reduce salmonella in poultry products.bacteriophages represent a group of viruses that specifically infect and replicate in bacteria and could potentially be used to reduce recovery of salmonella from poultry carcasses. bacteriophages were isolated from municipal wastewater in the presence of salmonella enteritidis phage type 13a (se). in the first 2 experiments, commercially processed broiler carcass rinse water was pooled and divided. the addition of 10(10) pfu/ml of a single bacteriophage (phl 4) with selected concentrations of s ...200516050131
recurrent bacteraemia caused by different salmonella species in a systemic lupus erythematosus patient.non-typhoidal salmonella infections are increasingly being encountered. recurrent systemic salmonellosis has previously been reported in both immunocompromised and immunocompetent hosts. however, recurrent salmonella bacteraemia by different species of salmonella has rarely been described. we report a patient with systemic lupus erythematosus having salmonella enteritidis and salmonella typhimurium infections after ingesting raw eggs and vegetables. multiple internal organs, including left knee ...200516035260
plasmid-mediated resistance to ciprofloxacin and cefotaxime in clinical isolates of salmonella enterica serotype enteritidis in hong kong.to characterize the genetic determinants involved in the reduced susceptibility to ciprofloxacin and cefotaxime in salmonella enterica serotype enteritidis clinical isolates obtained from four patients in summer 2003 in hong kong.200516033804
quantification of escherichia coli by kinetic 5'-nuclease polymerase chain reaction (real-time pcr) oriented to sfmd gene.a kinetic 5'-nuclease polymerase chain reaction (real-time pcr) for the quantification of escherichia coli was developed.200516033509
use of fourier transform infrared spectra of crude bacterial lipopolysaccharides and chemometrics for differentiation of salmonella enterica serotypes.to evaluate fourier transform infrared spectroscopy (ftir) and chemometrics for differentiating intact cells and crude lipopolysaccharide (lps) extracts from salmonella serotypes.200516033474
experimental salmonella enteritidis phage type 4 infection and egg transmission in japanese quails.japanese quails were inoculated orally with 1 x 10(10) colony-forming units of salmonella enteritidis phage type 4 isolated from chicks to obtain information concerning s. enteritidis infection and egg transmission. the inoculation resulted in a bacteraemic infection with seeding of the liver, spleen, intestine, peritoneum, ovule, ovary and oviduct. some infected birds showed diarrhoea, ruffled feathers, depression, loss of appetite and death. however, most infected birds remained clinically nor ...199216031673
a fatal disease outbreak in sheep associated with salmonella enteritidis ser havana.a disease outbreak with a mortality rate of 50% in a mob of wether lambs is described. salmonella enteritidis ser havana and pathogenic levels of trichostrongylus sp. were implicated as causal agents.198216030881
[an outbreak of salmonellosis in the kindergarten]. 200516028535
salmonella stress management and its relevance to behaviour during intestinal colonisation and infection.the enteric pathogen salmonella enterica is exposed to a number of stressful environments during its life cycle within and outside its various hosts. during intestinal colonisation salmonella is successively exposed to acid ph in the stomach, to the detergent-like activity of bile, to decreasing oxygen supply, to the presence of multiple metabolites produced by the normal gut microflora and finally it is exposed to cationic antimicrobial peptides present on the surface of epithelial cells. there ...200516023758
differential responses of macrophages to salmonella enterica serovars enteritidis and typhimurium.macrophages are major effectors against salmonella infection, and also transport bacteria between host tissues and provide a protected site for intracellular bacterial replication. we hypothesized that differences in chicken macrophage responses to salmonella enterica serovar enteritidis (se) and serovar typhimurium (st) played a role in preferential infection of eggs by se compared with st. to test this hypothesis, we determined bacterial phagocytosis and intracellular viability and macrophage ...200516023220
dyspepsia and irritable bowel syndrome after a salmonella gastroenteritis outbreak: one-year follow-up cohort study.it has been reported that some patients develop functional digestive disorders, particularly irritable bowel syndrome (ibs), after acute gastroenteritis (age). however, the presence of dyspepsia has not been specifically addressed. we prospectively evaluated development of dyspepsia and ibs during a 1-year follow-up in a cohort of adult patients affected by a salmonella enteritidis age outbreak.200516012939
rapid decay of salmonella flagella antibodies during human gastroenteritis: a follow up study.an indirect enzyme-linked immunosorbent assay (elisa) based on salmonella re-polymerized flagella was employed to measure levels of immunoglobulin (ig) g, igm and iga antibodies in sera from 303 danish patients diagnosed with either salmonella enteritidis or salmonella typhimurium. the antibody-levels were assessed at one, three and six months after onset of salmonellosis, and sera from a control-group of 170 healthy blood donors were additionally analysed in order to establish cut-off values fo ...200516009280
pheno-genotyping of salmonella enterica serotype enteritidis isolates identified in sicily during a reemergence period.after an upward trend paralleling that occurring in most european countries, including italy, since october 2002 salmonella enterica serotype enteritidis (s. enteritidis) has again gained the first position among outbreak and sporadic human isolates of salmonella in sicily. because phage typing of s. enteritidis has many technical and epidemiological limitations and molecular methods have proved to be poorly discriminative for this organism, multiple typing, using phage typing together with puls ...200415992280
aetiology and pathogenesis of reactive arthritis: role of non-antigen-presenting effects of hla-b27.spondyloarthropathies are inflammatory diseases closely associated with human leukocyte antigen (hla)-b27 by unknown mechanisms. one of these diseases is reactive arthritis (rea), which is typically triggered by gram-negative bacteria, which have lipopolysaccharide as an integral component of their outer membrane. several findings in vivo and in vitro obtained from patients with rea and from different model systems suggest that hla-b27 modulates the interaction between rea-triggering bacteria an ...200515987495
in vitro antimicrobial activity of extracts of four achillea species: the composition of achillea clavennae l. (asteraceae) extract.the extracts of aerial parts of achillea clavennae, achillea holosericea, achillea lingulata and achillea millefolium (hexane:ether:methanol=1:1:1) have been tested for antimicrobial activity in a disk diffusion assay against five bacteria (staphylococcus aureus, escherichia coli, klebsiella pneumoniae, pseudomonas aeruginosa and salmonella enteritidis) and two fungi (aspergillus niger and candida albicans). extracts of all four species possessed a broad spectrum of antimicrobial activity agains ...200515978758
[molecular epidemiology of salmonella enteritidis food poisoning by pulsed-field gel electrophoresis].during the years 1983 to 1999, a total of 120 salmonella enteritidis (se) isolates from various sources, patient's stool, foods, kitchen wear, river water etc., in 61 cases of food poisoning in the sakai city, were typed by pulsed-field gel electrophoresis (pfge) after xbai or noti digestion of chromosomal dna. xbai and noti restriction produced 2 (x1 and x2) and 3 (n1, n2 and n3) pulse-field profiles, respectively. the x1 and n1 types were further divided into 8 (xla-xlh) and 6 (n1a-n1f) subtyp ...200515977549
mapping of interactions and mouse congenic strains identified novel epistatic qtls controlling the persistence of salmonella enteritidis in mice.the host response to infection in humans is multifactorial and involves the complex interaction between two genomes (the host and the pathogen) and the environment. using an experimental mouse model of chronic infection, we have previously identified the individual effect of three significant and one suggestive quantitative trait loci (qtls) (ses1, ses2, ses3 and ses1.1) on salmonella enteritidis persistence in target organs of 129s6/svevtac mice. congenic strain construction was performed by tr ...200515973461
a temporal study of salmonella serovars in animals in alberta between 1990 and 2001.passive laboratory-based surveillance data from alberta agriculture food and rural development were analyzed for common salmonella serovars, prevalences, trends, and for the presence of temporal clusters. there were 1767 isolates between october 1990 and december 2001 comprising 63 different serovars, including 961 isolates from chickens, 418 from cattle, 108 from pigs, 102 from turkeys, and 178 from all other species combined. salmonella typhimurium, heidelberg, hadar, kentucky, and thompson we ...200515971672
salmonella-like bioadhesive nanoparticles.the aim of this work was to evaluate the bioadhesive potential of a polymeric vector obtained by the association between gantrez an nanoparticles and flagella-enriched salmonella enteritidis extract. fluorescently labelled nanoparticles (se-np) were prepared, after incubation between the polymer and the extract, by a solvent displacement method and cross-linkage with 1,3-diaminopropane. se-np displayed a size close to 280 nm and the amount of associated bacterial extract was 18 mug/mg nanopartic ...200515970347
binding of porcine ficolin-alpha to lipopolysaccharides from gram-negative bacteria and lipoteichoic acids from gram-positive bacteria.protein(s) reactive with n-acetyl-d-glucosamine (glcnac) was isolated from porcine nonimmune serum. the molecular weight of the purified protein was found to be mainly 40 kda on sodium dodecyl sulfate-polyacrylamide gel electrophoresis under reducing conditions. the n-terminal 10 amino acid sequence of the purified protein were found to be identical to that of porcine ficolin-alpha reported previously. in enzyme-linked immunosorbent assay, the purified protein was found to react with lipopolysac ...200615964070
development of a pcr system for the characterisation of salmonella flagellin genes.analysis of flagellin genes was carried out on strains of salmonella typhimurium, salmonella hadar, salmonella abortusequi, salmonella enteritidis and salmonella gallinarum serovars, using a pcr system designed in this study. the purpose of these studies was to explore the flagellin genes of biphasic and monophasic salmonellae for future targeted genetic interventions. the pcr primers were designed for two different structural genes of flagellin (flic, fljb), for the repressor of flic (flja), fo ...200515959975
anti-inflammatory properties of heat shock protein 70 and butyrate on salmonella-induced interleukin-8 secretion in enterocyte-like caco-2 cells.intestinal epithelial cells secrete the chemokine interleukin (il)-8 in the course of inflammation. because heat shock proteins (hsps) and butyrate confer protection to enterocytes, we investigated whether they modulate salmonella enterica serovar enteritidis (s. serovar enteritidis)-induced secretion of il-8 in enterocyte-like caco-2 cells. caco-2 cells incubated with or without butyrate (0-20 m m, 48 h) were infected with s. serovar enteritidis after (1 h at 42 degrees c, 6 h at 37 degrees c) ...200515958071
growth of salmonella in egg white.six non-s. enteritidis strains and eleven s. enteritidis strains of which 8 were isolated from eggs, were tested on their growth behaviour in fresh eggs and in eggs of 2 and 3 weeks old hold at 20 degrees c. the growth of salmonella at 20 degrees c was measured at days 6, 13 and 23 after inoculation. taking the average growth of the different s. enteritidis and of the non-s. enteritidis strains, we found that both groups grew very well in fresh eggs. s. enteritidis strains grew as good in eggs o ...200115954649
endotoxin releases a substance from the aorta that dilates an isolated arteriole by up-regulating inos.loss of vascular tone in resistance arterioles has been implicated as the cause of hypotension in septic shock. it is believed that the overproduction of nitric oxide (no) by the inducible isoform of nitric oxide synthase (inos) results in the vasodilatation seen in septic shock. however, we have shown that endotoxin has no effect on vascular tone of an isolated resistance vessel unless the endotoxin flows over a segment of aorta or vena cava upstream in the superfusion line. the aim of this stu ...200515921695
identification of ctx-m-14 {beta}-lactamase in a salmonella enterica serovar enteritidis isolate from japan.we analyzed the resistance to cefotaxime of a salmonella enterica serovar enteritidis isolate from a stool culture of a 4-year-old boy. it produced a beta-lactamase ctx-m-14, encoded by two related r plasmids. the region surrounding the blactx-m-14 gene had an original mosaic structure containing insertion sequences (is26 and is903d).200515917576
ovarian laying hen follicular maturation and in vitro salmonella internalization.transovarian transmission of paratyphoid salmonella is well documented and occurs at a low incidence in chickens. however, the exact mechanism of follicular invasion is not well understood. the following study investigates the ability of salmonella to invade ovarian follicles at different stages of follicular maturity in vitro. ovarian follicles were collected from leghorn hens and separated into three stages of maturity: (1) large yellow follicles or f follicles (lyf), (2) small yellow follicle ...200515917137
application of sybr green real-time pcr assay for specific detection of salmonella spp. in dairy farm environmental samples.the objective of this study was to develop and evaluate a sybr green 1 real-time pcr method for the specific detection of salmonella spp. in dairy farm environmental samples. previously reported 119-bp inva gene was selected for specificity, and 124 salmonella spp. including type strains and 116 non-salmonella strains were evaluated. all salmonella strains tested were inva-positive and all non-salmonella strains yielded no amplification products. the melting temperature (tm=79 degrees c) was con ...200515913820
identification of antiadhesive fraction(s) in nonimmunized egg yolk powder: in vitro study.the presence of antiadhesive component(s) in the hen egg yolk against foodborne pathogens was anticipated from results of a previous animal study conducted by the authors. the previous work showed egg yolk powder without specific antibodies is effective in controlling salmonella enteritidis,salmonella typhimurium, and escherichia coli o157:h7 colonization in laying hens. therefore, this study was necessary to locate the activity and identify the effective component(s). in vitro experiments were ...200515913333
mesenteric lymphangioma infected with non-typhoidal salmonella: the first case. 200515906046
communicable disease and health protection quarterly review: october to december 2004. from the health protection agency, centre for infections. 200515899905
prewashing with acidified sodium chlorite reduces pathogenic bacteria in lightly fermented chinese cabbage.efficacy of prewashing with acidified sodium chlorite (asc) for the sanitation of lightly fermented chinese cabbage was evaluated. the population of the natural microflora on the cabbage leaves was reduced about 2.0 log cfu/g just after washing with asc, a significant reduction compared with the control distilled water wash (p < or = 0.05). in the control experiment, viable aerobic bacteria increased gradually when incubated at 10 degrees c; however, asc-washed cabbage maintained a lower microbi ...200515895733
efficacy of electrolyzed water in inactivating salmonella enteritidis and listeria monocytogenes on shell eggs.the efficacy of acidic electrolyzed (eo) water produced at three levels of total available chlorine (16, 41, and 77 mg/ liter) and chlorinated water with 45 and 200 mg/liter of residual chlorine was investigated for inactivating salmonella enteritidis and listeria monocytogenes on shell eggs. an increasing reduction in listeria population was observed with increasing chlorine concentration from 16 to 77 mg/liter and treatment time from 1 to 5 min, resulting in a maximal reduction of 3.70 log cfu ...200515895731
identification of factors involved in recovery of heat-injured salmonella enteritidis.proteins and genes involved in the recovery of heat-injured salmonella enteritidis were investigated. salmonella enteritidis cells cultured overnight in tryptic soy broth (tsb; nonselective medium) were suspended in citric acid-disodium hydrogen phosphate buffer (ph 6). after heat treatment at 55 degrees c for 15 min, the culturable counts measured by tryptic soy agar (tsa; nonselective medium) decreased from 10(8) to 10(7) cfu/ml. on the other hand, culturable counts measured by desoxycholate-h ...200515895724
trends in nalidixic acid resistance in nontyphoidal salmonella isolated from 1999 to 2002: decreased susceptibility to 6 fluoroquinolones.we determined the resistance to nalidixic acid and 6 fluoroquinolones of 771 nontyphoidal salmonella strains isolated from humans between 1999 and 2002. a total of 22 different serotypes were identified among the salmonella isolates, the most common being salmonella enteritidis (79%) and salmonella derby (8%). resistance to nalidixic acid increased from 38% in 1999 to 43% in 2002. this resistance was not homogeneous among the different serotypes, with the highest percentage of resistant isolates ...200515893900
antagonistic activity of a natural fungal population towards pathogenic bacteria. an in vitro study.in the present work, we performed in vitro testing of 33 species of fungi of the subdivision deuteromycotina isolated from water and sediment of the kolubara river for antagonistic action towards 11 species of pathogenic bacteria. of gram-negative bacteria, the species most sensitive to metabolic fluid of the fungi were proteus mirabilis, salmonella enteritidis, and shigella sonnei, while the most resistant were klebsiella pneumoniae and salmonella typhimurium. of gram-positive bacteria, the mos ...200515889346
a mixed self-assembled monolayer-based surface plasmon immunosensor for detection of e. coli o157:h7.the sensitivity and specificity of a polyethylene glycol terminated alkanethiol mixed self-assembled monolayers (sam) on surface plasmon resonance (spr) immunosensor to detect escherichia coli o157:h7 is demonstrated. purified monoclonal (mabs) or polyclonal antibodies (pabs) against e. coli o157:h7 were immobilized on an activated sensor chip and direct and sandwich assays were carried to detect e. coli o157:h7. effect of protein g based detection and effect of concentrations of primary and sec ...200615878825
antimicrobial effect of lactic acid producing bacteria culture condensate mixture (lccm) against salmonella enteritidis.the antimicrobial effects of a lactic acid producing bacteria culture condensate mixture (lccm) were assessed against salmonella enteritidis. in the presence of lccm, bacterial growth was assessed in vitro by the measurement of optical density (od) and viable bacterial counting. at concentrations of 1.25 and 2.5% lccm, od values were significantly lower than that of the control broth, and at concentrations of 5 and 10% lccm, od values did not increase for the entire period of experiment. at 8 h ...200515878412
inactivation of enteric microorganisms with chemical disinfectants, uv irradiation and combined chemical/uv treatments.the relative disinfection efficiencies of peracetic acid (paa), hydrogen peroxide (h2o2) and sodium hypochlorite (naocl) against escherichia coli, enterococcus faecalis, salmonella enteritidis and coliphage ms2 virus were studied in laboratory-scale experiments. this study also evaluated the efficiency of combined paa/ultraviolet irradiation (uv) and h2o2/uv treatments to determine if the microbial inactivation was synergistic. microbial cultures were added into a synthetic wastewater-like test ...200515878023
salmonella enteritidis outbreak linked to a local bakery, british columbia, canada. 200515875325
clonal expansion may account for high levels of quinolone resistance in salmonella enterica serovar enteritidis.we have observed a high incidence of isolated nalidixic acid resistance in salmonella enterica serovar enteritidis isolates in ireland, particularly isolates of phage type 1 (pt1). a group of nalidixic acid-resistant (n = 22) and nalidixic acid-susceptible (n = 28) isolates of serovar enteritidis from multiple sites in ireland were selected. isolates were typed by pulsed-field gel electrophoresis (pfge) with xbai, and the mics for nalidixic acid and ciprofloxacin were determined. mutations assoc ...200515870349
potential role of diploscapter sp. strain lkc25, a bacterivorous nematode from soil, as a vector of food-borne pathogenic bacteria to preharvest fruits and vegetables.diploscapter, a thermotolerant, free-living soil bacterial-feeding nematode commonly found in compost, sewage, and agricultural soil in the united states, was studied to determine its potential role as a vehicle of salmonella enterica serotype poona, enterohemorrhagic escherichia coli o157:h7, and listeria monocytogenes in contaminating preharvest fruits and vegetables. the ability of diploscapter sp. strain lkc25 to survive on agar media, in cow manure, and in composted turkey manure and to be ...200515870330
recombinant chicken il-6 does not activate heterophils isolated from day-old chickens in vitro.pro-inflammatory cytokines are produced as part of innate immunity. increased resistance to extraintestinal salmonella enteritidis (se) has been associated with an increase in heterophil pro-inflammatory cytokine gene expression. invasion of chicken epithelial cells by se induces an 8- to 10-fold increase in interleukin (il) -6 production. infection with se induces an influx of heterophils to the site of infection; therefore, we hypothesize heterophils would be responsive to il-6. the objective ...200515859240
individual-based modelling of growth and migration of salmonella enteritidis in hens' eggs.an individual-based model (ibm) was developed to describe the growth and migration of salmonella enteritidis in hens' eggs. the bacteria simulator (bacsim) environment was used to implement the model; the bacteria are represented by spheres that grow by nutrient uptake and divide in two daughter cells upon exceeding a certain threshold volume. motility of the salmonella bacteria was described by a run and tumble mechanism. for the sake of simplicity, the bacteria were assumed to grow exponential ...200415854715
[acute pancreatitis due to salmonella enteritidis infection]. 200515847759
infection of the reproductive tract and eggs with salmonella enterica serovar pullorum in the chicken is associated with suppression of cellular immunity at sexual maturity.salmonella enterica serovar pullorum causes persistent infections in laying hens. splenic macrophages are the main site of persistence. at sexual maturity, numbers of bacteria increase and spread to the reproductive tract, which may result in vertical transmission to eggs or chicks. in this study we demonstrate that both male and female chickens may develop a carrier state following infection but that the increases in bacterial numbers and spread to the reproductive tract are phenomena restricte ...200515845505
emergence of salmonella epidemics: the problems related to salmonella enterica serotype enteritidis and multiple antibiotic resistance in other major serotypes.two major changes in the epidemiology of salmonellosis occurred in the second half of the 20th century: the emergence of food-borne human infections caused by s. enteritidis and by multiple-antibiotic resistant strains of salmonella. this review updates information on the s. enteritidis pandemic and focuses on the emergence of salmonella, carrying the sgi1 antibiotic resistance gene cluster, resistant to extended-spectrum cephalosporins, or resistant to fluoroquinolones. the factors responsible ...200515845226
penetration of salmonella enteritidis and salmonella heidelberg into egg yolks in an in vitro contamination model.eggs that harbor salmonella in their edible contents pose a significant risk of transmitting disease to consumers. although salmonella deposition inside yolks does not usually occur at a high frequency in naturally contaminated eggs, bacterial penetration through the vitelline membrane could lead to rapid and extensive multiplication in the nutrient-rich yolk contents. the present study used an in vitro egg contamination model to assess the ability of salmonella strains to penetrate the vitellin ...200515844820
eliminating the carriage of salmonella enterica serovar enteritidis in domestic fowls by feeding activated charcoal from bark containing wood vinegar liquid (nekka-rich).the protective efficacy of activated charcoal containing wood vinegar liquid (nekka-rich) against intestinal infection with salmonella enterica serovar enteritidis was sought. in the present study, the adsorption effects of activated charcoal of nekka-rich on s. enteritidis and normal bacterial flora in the intestine, enterococcus faecium, were examined. s. enteritidis was effectively adsorbed by activated charcoal of nekka-rich. on the other hand, activated charcoal of nekka-rich showed lower b ...200515844805
dose-dependent modulation of the in vitro cytokine production of human immune competent cells by lead salts.lead pollution constitutes a major health problem that has been intensively debated. to reveal its effects on the immune response, the influence of lead on the in vitro cytokine production of human peripheral mononuclear blood cells was investigated. isolated cells were exposed to lead acetate or lead chloride for 24 h in the presence of either heat-killed salmonella enteritidis (hk-se) or monoclonal antibodies (anti-cd3, anti-cd28, anti-cd40) as cell activators. our results showed that while hi ...200515843504
il-12 family members: differential kinetics of their tlr4-mediated induction by salmonella enteritidis and the impact of il-10 in bone marrow-derived macrophages.the members of the il-12 family of heterodimeric cytokines play a pivotal role in initiation and regulation of cell-mediated immunity. best known is il-12p70, which promotes an immune response towards t(h)1 bias. other members of this family (il-23, il-27) are less well characterized in terms of induction and function. using either heat-killed or viable salmonella enteritidis or lps as a stimulus, the kinetics of mrna production of each member of the il-12 family (p19, p28, p35, p40, ebstein-bar ...200515837713
precise excision and secondary transposition of tnphoa in non-motile mutants of a salmonella enterica serovar enteritidis clinical isolate.mutagenesis with tnphoa has been widely used in many bacteria. here, we report the excision and secondary transposition of this transposon in three non-motile (flic, flif and motb) mutants of salmonella enterica serovar enteritidis (s. enteritidis). isolation of motile revertants showed that they were kanamycin resistant and conserved a copy of tnphoa in their genome in an insertion site different from the initial one. they also expressed an intact flagella. characterization of the motile revert ...200515837381
effectiveness of trisodium phosphate treatment against pathogenic and spoilage bacteria on poultry during refrigerated storage.to determine the efficacy of trisodium phosphate (tsp) against pathogenic and spoilage bacteria on poultry and to assess the influence of the bacterial combination of inoculum on tsp effect, chicken legs were coinoculated with similar concentrations of a pathogenic (salmonella enterica ser. enteritidis or listeria monocytogenes) and a spoilage (pseudomonas fluorescens or brochothrix thermosphacta) bacteria. samples were dipped in tsp (12%, 15 min) or were not treated (control). microbiological a ...200515830686
destruction of salmonella senftenberg on the shells of intact eggs by thermoultrasonication.the combined effect of ultrasonic waves and heat treatment applied simultaneously was evaluated on the survival of strains of salmonella senftenberg on shells of intact eggs. this combined process has a higher killing effect than heat treatment alone. the decimal reduction times (d-values) were decreased by 65.2 to 11.1% in the temperature range studied (57.8 to 67 degrees c). in contrast to the effect on salmonella enterica serovar enteritidis in a previous study, thermoultrasonication had no i ...200515830681
growth of salmonella enterica serovar enteritidis in albumen and yolk contents of eggs inoculated with this organism onto the vitelline membrane.by using an in vitro model simulating the potential opportunities for salmonella enterica serovar enteritidis (se) to proliferate within eggs contaminated with this organism following oviposition, we investigated growth of se in eggs. seventy to 140 cfu of one of three se strains originating either from egg contents, chicken meat, or a human infection were experimentally inoculated onto the vitelline membrane of eggs collected from specific-pathogen-free flocks of chickens and incubated at 25 de ...200515830661
inactivation of salmonella enterica serovar enteritidis on shell eggs by ozone and uv radiation.the presence of salmonella enterica serovar enteritidis in shell eggs has serious public health implications. several treatments have been developed to control salmonella on eggs with mixed results. currently, there is a need for time-saving, economical, and effective egg sanitization treatments. in this study, shell eggs externally contaminated with salmonella (8.0 x 10(5) to 4.0 x 10(6) cfu/g of eggshell) were treated with gaseous ozone (o3) at 0 to 15 lb/in2 gauge for 0 to 20 min. in other ex ...200515830660
raw eggs--lessons learned from an outbreak of salmonella serotype enteritidis infections associated with meringue pie.we investigated a salmonellosis outbreak related to a cafeteria to determine its extent and to identify illness risk factors.200515829832
new antibacterial peptide derived from bovine hemoglobin.peptic digestion of bovine hemoglobin at low degree of hydrolysis yields an intermediate peptide fraction exhibiting antibacterial activity against micrococcus luteus a270, listeria innocua, escherichia coli and salmonella enteritidis after separation by reversed-phase hplc. from this fraction a pure peptide was isolated and analyzed by matrix-assisted laser desorption/ionization mass spectrometry (maldi-ms) and electrospray ionization tandem mass spectrometry (esi-ms/ms). this peptide correspon ...200515808900
haemorrhagic cellulitis caused by salmonella enteritidis.soft tissue infections are rare manifestations of extra-intestinal salmonellosis and occur more frequently in immunocompromized patients [1-4]. we report haemorrhagic cellulitis in an immunosuppressed patient with sepsis caused by a non-typhoid salmonella.200515804669
septic arthritis due to salmonella enteritidis associated with infliximab use.a unique case of septic arthritis caused by salmonella enteritidis in a patient receiving infliximab for rheumatoid arthritis is presented. antimicrobial chemotherapy with surgical intervention was necessary for eradication of the infection. physicians should be aware of rare manifestations of salmonella infections associated with infliximab use, especially in endemic areas.200515804667
infectious bursal disease virus-induced immunosuppression in the chick is associated with the presence of undifferentiated follicles in the recovering bursa.infectious bursal disease virus (ibdv) causes an acute cytolytic infection in chicken b lymphocytes resulting in destruction of the b-cell population. most severe depletion occurs in the bursa of fabricius, where the immunoglobulin repertoire is developed by gene conversion. chicks surviving ibdv infection are immunosuppressed despite repopulation of the bursa with b cells. here we show that infection of neonatal chicks with a classical virulent ibdv strain (f52/70) causes severe bursal bcell de ...200515802957
tumour-like inflammatory abdominal conditions in children.the presence of a tumour, poor general condition, features of anaemia, increased erythrocyte sedimentation rates and imaging suggesting malignancy were the common features in 4 different tumour-like abdominal conditions that are extremely rare in childhood. these conditions included: extensive retroperitoneal tumour with rib involvement that turned out to be an inflammatory lesion caused by actinomyces in a 12-year-old girl; multi-loculated tumour of the mesentery/ovary caused by mesenteric lymp ...200515795826
dietary fructooligosaccharides increase intestinal permeability in rats.we showed previously that fructooligosaccharides (fos) decrease the resistance to salmonella infection in rats. however, the mechanism responsible for this effect is unclear. therefore, we examined whether dietary fos affects intestinal permeability before and after infection with salmonella enterica serovar enteritidis. male wistar rats were fed restricted quantities of a purified diet that mimicked the composition of a western human diet. the diet was supplemented with 60 g/kg cellulose (contr ...200515795444
an attenuated salmonella enteritidis strain derivative of the main genotype circulating in uruguay is an effective vaccine for chickens.we have recently reported that salmonella enterica serovar enteritidis (s. enteritidis) strains circulating in uruguay, are unevenly distributed among different genetic subtypes, with a predominant genotype that is a common contaminant of poultry-derived food and that accounts for the vast majority of human cases of food-borne disease. herein, we describe the construction of a genetically-defined aroc derivative (lvr02) of a local strain of s. enteritidis belonging to the major genetic type. we ...200515795080
[outbreak of salmonella enteriditis food poisoning at a dining hall facility].an outbreak of salmonella enteriditis food poisoning having occurred at two banquet halls, affecting eight groups of guests, is described. this research was aimed at determining the food by means of which this illness was transmitted.200515794585
molecular epidemiology of salmonella enterica serovar enteritidis strains by pulsed-field gel electrophoresis isolated in the slovak republic.a total 44 isolates of s. enterica serovar enteritidis (s. enteritidis) belonged to three different phage types (pts) 9a, 13a, 25 were analyzed by the technique of pulsed-field gel electrophoresis (pfge). thirty and three strains were from two outbreaks (central and southern regions of the slovak republic pts 13a, 25) and 11 isolates were sporadic isolates (pt9a). these isolates showed two different patterns in pfge with xbai digestion. strains of pt13a generated pfp a and isolates of pt25 showe ...200415790080
development of a sandwich elisa for the detection of listeria spp. using specific flagella antibodies.five monoclonal antibodies (mabs) and chicken immunoglobulin (igy) were developed by immunizing with flagella purified from listeria monocytogenes 4b and the five mabs have been confirmed to be specific against three different epitopes of flagellin. the antibodies showed specific reaction to listeria genus and no cross-reactivity with other bacteria tested in this experiment including e.coli o157:h7 and salmonella enteritidis. sandwich enzyme-linked immunosorbent assays (elisa) using the mabs an ...200515785122
epidemiological and epizootological aspects of salmonellosis.in their case report, most of the patients with salmonellosis mentioned consumption of meals containing poultry products, primarily eggs and egg products as a source of their disease. microbiological analysis of samples showed that in more than 88 % of cases the infection agent was identified as salmonella enteritidis. this serovar is the most frequent pathogen isolated at poultry farms. in the period of the past five years, 171 outbreaks of salmonellosis were recorded in slovakia. however, with ...200415777071
antimicrobial resistance patterns and serotype distribution among salmonella enterica strains in turkey, 2000-2002.since turkey currently lacks a national reference center for salmonella infections, the present study was conducted to document the distribution of serotypes and antimicrobial resistance patterns among salmonella enterica isolates recovered from clinical samples in ten turkish provinces over a 2-year period. among the 620 salmonella enterica isolates recovered between 1 july 2000 and 30 june 2002, strains belonging to the serotypes enteritidis (47.7%), typhimurium (34.7%), paratyphi b (6.0%), ty ...200515772822
antibiogram studies of salmonella enteritidis phage type 4 isolates from poultry and meat.human infection with salmonella enteritidis phage type 4 has increased worldwide since last decade and has been shown to be related mainly with the consumption of poultry meat and eggs. the public health significance and economic importance of this serovar underscores the need to generate base line data on the antimicrobial susceptibilities and protein profile of indigenous s. enteritidis. this study was performed to investigate the antimicrobial susceptibilities of s. enteritidis phagetype4 iso ...200415762066
[some epidemiologic characteristics of foodborne intoxications in croatia during the 1992-2001 period].epidemiologic characteristics of foodborne intoxications registered in croatia during the 1992-2001 period are described. salmonellosis, as well as staphylococcal food intoxication, foodborne botulism, clostridium perfringens food intoxication, histamine poisoning and food intoxication caused by other pathogens, especially conditionally pathogenic bacteria, were analyzed. during the study period, 87,782 cases were reported, 45,721 (52.1%) of them related to salmonellosis and the rest of 42,061 ( ...200415756811
bifidobacterium lactis sp. 420 up-regulates cyclooxygenase (cox)-1 and down-regulates cox-2 gene expression in a caco-2 cell culture model.cyclooxygenases (cox) -1 and -2 play important roles in gastrointestinal health; chronic overexpression of cox-2 is associated with inflammatory and cancerous disease, whereas cox-1 is expressed constitutively. we studied the effects of two probiotic (bifidobacterium lactis sp. 420 and lactobacillus acidophilus) and two control microorganisms (escherichia coli and salmonella enteritidis) and four microbial metabolites (acetate, butyrate, lactate and propionate) on the expression levels of the co ...200515749634
performance and nutrient retention responses of broilers to dietary oxyhalogenic and ionic salts.two experiments were conducted to determine the effects of 2 ionic and antimicrobial mixtures on broiler performance and nutrient retention. in experiment 1, male broilers were fed 6 diets in a 2 x 3 factorial experiment (5 cages/diet, 9 chicks/cage) from 0 to 21d of age. diets with 2 nutrient densities [normal industry diet (nd) and a low nutrient density diet (82% of nd)] and 3 ionic and antimicrobial mixtures [none (control) or 1 of 2 formulations containing different mixtures of ionic salts ...200515742960
effects of drinking water treatment on susceptibility of laying hens to salmonella enteritidis during forced molt.feed deprivation is used in the layer industry to induce molting and stimulate multiple egg-laying cycles in laying hens. unfortunately, the stress involved increases susceptibility to salmonella enteritidis (se), the risk of se-positive eggs, and incidence of se in internal organs. leghorn hens over 50 wk of age were divided into 4 treatment groups of 12 hens each in experiment 1 and 3 treatment groups of 12 hens in experiments 2 and 3; hens were placed in individual laying hen cages. treatment ...200515742955
reduction of salmonella enterica serovar enteritidis colonization and invasion by an alfalfa diet during molt in leghorn hens.the standard method for molting to stimulate multiple egg-laying cycles in laying hens is feed deprivation. however, the physiological changes within hens caused by feed deprivation increase susceptibility of the hens to salmonella enterica serovar enteritidis (se) infection. in an effort to develop an alternative method to induce molting without increasing susceptibility to se, an alfalfa diet was compared with the standard molting method for the level of ovary regression and se colonization. h ...200515742953
comparison of immunosuppression in dry and lactating awassi ewes due to water deprivation stress.in seminomadic farming practice, dry and lactating ewes are exposed to different degrees of water deprivation, leading to stress followed by various disease outbreaks. this study compares quantitatively the immunosuppression to salmonella enteritidis (se) fimbriae (14 and 21 kda) and other major polypeptides (28.9, 37.7, 42.9, 68.0, 92.6 and 96.8 kda) in water-deprived dry and lactating ewes. sixteen dry and lactating multiparous awassi ewes were divided into four treatment groups (a, a', b and ...200515727291
computational fluid dynamics analysis for process impact assessment during thermal pasteurization of intact eggs.transient temperature and albumen velocity profiles during thermal pasteurization of intact eggs were studied using a commercial computational fluid dynamics (cfd) package. simulated temperature profiles were in close agreement with experimental data for eggs of different sizes. convective heat transfer only occurred in the egg white fraction, and conductive heat transfer only occurred in the yolk. for process assessment, a generally accepted kinetic inactivation model for salmonella enteritidis ...200515726983
survival of pathogenic bacteria in pesticide solutions and on treated tomato plants.the ability of salmonella, escherichia coli o157:h7, listeria monocytogenes, and shigella to survive or grow in pesticide solutions (ambush 240ec, benlate t-n-g, bravo 500, botran 75wp, captan 80wdg, parasol, and vendex 50w) used by the horticultural industry was examined. in the laboratory, individual cultures were inoculated at 4 log cfu/ml in pesticides diluted with sterile saline to the lowest recommended spray concentrations. during 21 degrees c incubation for < or =96 h, bacterial survivor ...200515726972
salmonella and raw shell eggs: results of a cross-sectional study of contamination rates and egg safety practices in the united kingdom catering sector in 2003.this study was prompted by epidemiological investigations of the unusual number of salmonella enteritidis outbreaks associated with the use of eggs in catering premises in england and wales during 2002. the aims of the study, performed between april and may 2003, were to establish the rate of salmonella contamination in raw shell eggs from catering premises, investigate any correlation between the origin and type of eggs and the presence of particular serotypes or phage types (pts) of salmonella ...200515726966
comparison of multidrug resistance gene regions between two geographically unrelated salmonella serotypes.the aim of this study was to identify chromosomally integrated genes conferring multidrug resistance to a salmonella enterica (s.) serotype typhimurium isolate, phage type dt193, isolated in ireland and to compare them with resistance genes conferring plasmid-mediated multidrug resistance to a s. enteritidis isolate from italy.200515722395
screening of lps-specific peptides from a phage display library using epoxy beads.the selection of identical or highly homologous peptides from phage display combinatorial peptide libraries has been unsuccessful in biopanning experiments using microtiter plates. in the present study, by biopanning on lps-conjugated epoxy beads, we repeatedly enriched clones encoding awlpwak and nlqeflf. these peptides were found to interact with the polysaccharide moiety of lps, which is highly variable among gram negative bacterial species. in addition, phages encoding these peptides prefere ...200515721308
[salmonella aortitis. a rare cause of fever and backache]. 200515719308
estimate of illnesses from salmonella enteritidis in eggs, united states, 2000.results from our model suggest that eating salmonella enterica serovar enteritidis-contaminated shell eggs caused 182,060 illnesses in the united states during 2000. uncertainty about the estimate ranged from 81,535 (5th percentile) to 276,500 illnesses (95th percentile). our model provides but 1 approach for estimating foodborne illness and quantifying estimate uncertainty.200515705332
spectroscopic characterization of microorganisms by fourier transform infrared microspectroscopy.spectroscopic fingerprints of bacteria were investigated by fourier transform infrared (ftir) microspectroscopy for the elucidation of chemical composition and structural information during growth. good differentiation of six microorganisms was achieved down to the strain level. the inherent compositional and structural differences of cell envelopes and cytoplasm were investigated and utilized to obtain more detailed analysis of the spectroscopic features. bands or regions of key functional grou ...200515700299
nontyphoid salmonellosis in polish children. 200515699707
purified beta-glucan as an abiotic feed additive up-regulates the innate immune response in immature chickens against salmonella enterica serovar enteritidis.functionally, the innate immune system of immature chickens is inefficient during the first week posthatch. this immunological inefficiency enables pathogens such as salmonella enterica serovar enteritidis (se) to invade and colonize the visceral organs of immature chickens. the objective of this study was to evaluate the effect of purified beta-glucan as an immunomodulator of the innate immune response. beta-glucan, as a feed additive, significantly provided protection against se organ invasion ...200515698692
ifn-gamma priming of chicken heterophils upregulates the expression of proinflammatory and th1 cytokine mrna following receptor-mediated phagocytosis of salmonella enterica serovar enteritidis.responsiveness to invasive pathogens, clearance via the inflammatory response, and activation of appropriate acquired responses are all coordinated by innate host defenses. polymorphonuclear leukocytes (pmns) are cellular components of innate response, with the primary pmn in poultry being the heterophil. priming is the potentiation of the phagocyte activation process. interferon-gamma (ifn-gamma) is a pleiotropic cytokine involved in basically all phases of immune and inflammatory responses tha ...200515695928
an international outbreak of salmonellosis associated with raw almonds contaminated with a rare phage type of salmonella enteritidis.during the winter of 2000 to 2001, an outbreak due to salmonella enteritidis (se) phage type 30 (pt30), a rare strain, was detected in canada. the ensuing investigation involved canadian and american public health and food regulatory agencies and an academic research laboratory. enhanced laboratory surveillance, including phage typing and pulsed-field gel electrophoresis, was used to identify cases. case questionnaires were administered to collect information about food and environmental exposur ...200515690826
heterophils are associated with resistance to systemic salmonella enteritidis infections in genetically distinct chicken lines.heterophils mediate acute protection against salmonella in young poultry. we evaluated susceptibility of genetically distinct lines of broilers to systemic salmonella enteritidis (se) infections. se was administered into the abdomen of day-old chickens (parental lines [a and b]; f1 reciprocal crosses [c and d]) to assess modulation of leukocytes and survivability of chickens. line a was more resistant to se than line b; likewise cross d was more resistant than cross c. significantly more heterop ...200515681144
Displaying items 2301 - 2400 of 6195