Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| maintenance of demographic and hematological profiles in a long-lasting dengue fever outbreak: implications for management. | dengue fever (df) outbreaks present regionally specific epidemiological and clinical characteristics. in certain medium-sized cities (100 000-250 000 inhabitants) of são paulo state, brazil, and after reaching an incidence of 150 cases/100 000 inhabitants ("epidemiological threshold"), clinical diagnosis indicated dengue virus (denv) infection. during this period, other seasonally infectious diseases with symptoms and physical signs mimicking df can simultaneously occur, with the consequential o ... | 2016 | 27593529 |
| non-human primate antibody response to mosquito salivary proteins: implications for dengue virus transmission in puerto rico. | an important step to incriminate a mosquito as a vector of a disease pathogen is finding evidence of direct contact between the mosquito and humans. typically, this is accomplished through landing/biting catches, or host blood meal analysis in engorged mosquitoes via immunologic assays. an alternate approach is to identify the presence of specific mosquito anti-saliva protein antibodies in the blood of exposed hosts. following the discovery of dengue infected, free roaming non-human primates in ... | 2016 | 27593498 |
| identification and expression analysis of two 14-3-3 proteins in the mosquito aedes aegypti, an important arboviruses vector. | the 14-3-3 proteins are evolutionarily conserved acidic proteins that form a family with several isoforms in many cell types of plants and animals. in invertebrates, including dipteran and lepidopteran insects, only two isoforms have been reported. 14-3-3 proteins are scaffold molecules that form homo- or heterodimeric complexes, acting as molecular adaptors mediating phosphorylation-dependent interactions with signaling molecules involved in immunity, cell differentiation, cell cycle, prolifera ... | 2016 | 27592842 |
| aedes aegypti (diptera: culicidae) longevity and differential emergence of dengue fever in two cities in sonora, mexico. | dengue virus, primarily transmitted by the aedes aegypti (l.) mosquito, has rapidly expanded in geographic extent over the past several decades. in some areas, however, dengue fever has not emerged despite established ae. aegypti populations. the reasons for this are unclear and have sometimes been attributed to socio-economic differences. in 2013 we compared ae. aegypti adult density and population age structure between two cities in sonora, mexico: hermosillo, which has regular seasonal dengue ... | 2016 | 27590231 |
| nonstructural proteins are preferential positive selection targets in zika virus and related flaviviruses. | the flavivirus genus comprises several human pathogens such as dengue virus (denv), japanese encephalitis virus (jev), and zika virus (zikv). although zikv usually causes mild symptoms, growing evidence is linking it to congenital birth defects and to increased risk of guillain-barré syndrome. zikv encodes a polyprotein that is processed to produce three structural and seven nonstructural (ns) proteins. we investigated the evolution of the viral polyprotein in zikv and in related flaviviruses (d ... | 2016 | 27588756 |
| a comprehensive systems biology approach to studying zika virus. | zika virus (zikv) is responsible for an ongoing and intensifying epidemic in the western hemisphere. we examined the complete predicted proteomes, glycomes, and selectomes of 33 zikv strains representing temporally diverse members of the african lineage, the asian lineage, and the current outbreak in the americas. derivation of the complete selectome is an 'omics' approach to identify distinct evolutionary pressures acting on different features of an organism. employment of the m8 model did not ... | 2016 | 27584813 |
| genetic evidence of expansion by passive transport of aedes (stegomyia) aegypti in eastern argentina. | 2016 | 27583521 | |
| convergent evolution of trna gene targeting preferences in compact genomes. | in gene-dense genomes, mobile elements are confronted with highly selective pressure to amplify without causing excessive damage to the host. the targeting of trna genes as potentially safe integration sites has been developed by retrotransposons in various organisms such as the social amoeba dictyostelium discoideum and the yeast saccharomyces cerevisiae. in d. discoideum, trna gene-targeting retrotransposons have expanded to approximately 3 % of the genome. recently obtained genome sequences o ... | 2016 | 27583033 |
| transcriptional analysis of the adaptive digestive system of the migratory locust in response to plant defensive protease inhibitors. | herbivorous insects evolved adaptive mechanisms to compensate for the presence of plant defensive protease inhibitors (pi) in their food. the underlying regulatory mechanisms of these compensatory responses remain largely elusive. in the current study, we investigated the initiation of this adaptive response in the migratory locust, locusta migratoria, via microarray analysis of gut tissues. four hours after dietary uptake of pis, 114 and 150 transcripts were respectively found up- or downregula ... | 2016 | 27581362 |
| zika in the united states: how are we preparing? | 0 | 27581255 | |
| bacterial diversity of the american sand fly lutzomyia intermedia using high-throughput metagenomic sequencing. | parasites of the genus leishmania cause a broad spectrum of diseases, collectively known as leishmaniasis, in humans worldwide. american cutaneous leishmaniasis is a neglected disease transmitted by sand fly vectors including lutzomyia intermedia, a proven vector. the female sand fly can acquire or deliver leishmania spp. parasites while feeding on a blood meal, which is required for nutrition, egg development and survival. the microbiota composition and abundance varies by food source, life sta ... | 2016 | 27581188 |
| molecular signatures associated with zikv exposure in human cortical neural progenitors. | zika virus (zikv) infection causes microcephaly and has been linked to other brain abnormalities. how zikv impairs brain development and function is unclear. here we systematically profiled transcriptomes of human neural progenitor cells exposed to asian zikv(c), african zikv(m), and dengue virus (denv). in contrast to the robust global transcriptome changes induced by denv, zikv has a more selective and larger impact on expression of genes involved in dna replication and repair. while overall e ... | 2016 | 27580721 |
| virus-derived dna drives mosquito vector tolerance to arboviral infection. | mosquitoes develop long-lasting viral infections without substantial deleterious effects, despite high viral loads. this makes mosquitoes efficient vectors for emerging viral diseases with enormous burden on public health. how mosquitoes resist and/or tolerate these viruses is poorly understood. here we show that two species of aedes mosquitoes infected with two arboviruses from distinct families (dengue or chikungunya) generate a viral-derived dna (vdna) that is essential for mosquito survival ... | 2016 | 27580708 |
| effects of environment, dietary regime and ageing on the dengue vector microbiota: evidence of a core microbiota throughout aedes aegypti lifespan. | mosquito midgut microbiota is a key component of vector competence, as gut bacteria can disturb pathogen development. in this study, we addressed the microbiota composition of aedes aegypti during its lifespan, under field conditions. we also investigated the possible effects of environment, dietary regime and ageing on the gut community composition. we employed culture independent and dependent approaches to characterise vector microbiota. there was evidence of a lifelong stable core microbiota ... | 2016 | 27580348 |
| crygetter: a tool to automate retrieval and analysis of cry protein data. | for many years, the use of chemical agents to control crop pests has been degrading the environment, bringing problems to humans and all living things. an alternative to deal with the pests is the use of biopesticides, biological agents capable of controlling these harmful organisms. one kind of biopesticide is bacillus thuringiensis, a gram-positive bacterium that synthesizes a protein that, when ingested by the pests, kills them and does not harm other species. | 2016 | 27578522 |
| egg hatching and survival of immature stages of aedes aegypti (diptera: culicidae) under natural temperature conditions during the cold season in buenos aires, argentina. | in temperate regions, the seasonal dynamics of aedes aegypti (l.) (diptera: culicidae) is mainly influenced by temperature. it is assumed that, during the winter season, the population remains as eggs and that the development and population growth of surviving eggs begin during the following spring. the aim of the current study was to assess egg hatching of ae. aegypti during the winter in buenos aires city (argentina), and analyze the survival of immature stages. the experiments consisted of im ... | 2016 | 27576509 |
| vertical transmission of zika virus in aedes aegypti mosquitoes. | previous experimental studies have demonstrated that a number of mosquito-borne flavivirus pathogens are vertically transmitted in their insect vectors, providing a mechanism for these arboviruses to persist during adverse climatic conditions or in the absence of a susceptible vertebrate host. in this study, designed to test whether zika virus (zikv) could be vertically transmitted, female aedes aegypti and aedes albopictus were injected with zikv, and their f1 adult progeny were tested for zikv ... | 2016 | 27573623 |
| mosquito c-type lectins maintain gut microbiome homeostasis. | the long-term evolutionary interaction between the host immune system and symbiotic bacteria determines their cooperative rather than antagonistic relationship. it is known that commensal bacteria have evolved a number of mechanisms to manipulate the mammalian host immune system and maintain homeostasis. however, the strategies employed by the microbiome to overcome host immune responses in invertebrates still remain to be understood. here, we report that the gut microbiome in mosquitoes utilize ... | 2016 | 27572642 |
| transcriptional differences between diapausing and non-diapausing d. montana females reared under the same photoperiod and temperature. | a wide range of insects living at higher latitudes enter diapause at the end of the warm season, which increases their chances of survival through harsh winter conditions. in this study we used rna sequencing to identify genes involved in adult reproductive diapause in a northern fly species, drosophila montana. both diapausing and non-diapausing flies were reared under a critical day length and temperature, where about half of the emerging females enter diapause enabling us to eliminate the eff ... | 2016 | 27571415 |
| patterned array of poly(ethylene glycol) silane monolayer for label-free detection of dengue. | in the present study, the construction of arrays on silicon for naked-eye detection of dna dengue was demonstrated. the array was created by exposing a polyethylene glycol (peg) silane monolayer to 254 nm ultraviolet (uv) light through a photomask. formation of the peg silane monolayer and photomodifed surface properties was thoroughly characterized by using atomic force microscopy (afm), x-ray photoelectron spectroscopy (xps), and contact angle measurements. the results of xps confirmed that ir ... | 2016 | 27571080 |
| the correlation between dengue incidence and diurnal ranges of temperature of colombo district, sri lanka 2005-2014. | meteorological factors affect dengue transmission. mechanisms of the way in which different diurnal temperatures, ranging around different mean temperatures, influence dengue transmission were published after 2011. | 2016 | 27566717 |
| open-air biowarfare testing and the evolution of values. | the united states and the united kingdom ended outdoor biological warfare testing in populated areas nearly half a century ago. yet, the conduct, health effects, and propriety of those tests remain controversial. the varied views reflect the limits of currently available test information and evolving societal values on research involving human subjects. western political culture has changed since the early days of the american and british testing programs. people have become less reluctant to qu ... | 2016 | 27564984 |
| an ecological assessment of the pandemic threat of zika virus. | the current outbreak of zika virus poses a severe threat to human health. while the range of the virus has been cataloged growing slowly over the last 50 years, the recent explosive expansion in the americas indicates that the full potential distribution of zika remains uncertain. moreover, many studies rely on its similarity to dengue fever, a phylogenetically closely related disease of unknown ecological comparability. here we compile a comprehensive spatially-explicit occurrence dataset from ... | 2016 | 27564232 |
| flavivirus ns1 protein in infected host sera enhances viral acquisition by mosquitoes. | the arbovirus life cycle involves viral transfer between a vertebrate host and an arthropod vector, and acquisition of virus from an infected mammalian host by a vector is an essential step in this process. here, we report that flavivirus nonstructural protein-1 (ns1), which is abundantly secreted into the serum of an infected host, plays a critical role in flavivirus acquisition by mosquitoes. the presence of dengue virus (denv) and japanese encephalitis virus ns1s in the blood of infected inte ... | 2016 | 27562253 |
| toxicity and larvicidal activity of podophyllum-based lignans against aedes aegypti (diptera: culicidae). | aedes aegypti l. (diptera: culicidae) is a mosquito species that has adapted to urban environments and is the main vector of dengue viruses. because of the increasing incidence of dengue, a more environmentally acceptable insecticide needs to be found. natural products have been and continue to be an important source of leading compounds that can be modified in order to develop new drugs. the lignan family of natural products includes compounds with a diverse spectrum of biological activity. pod ... | 2016 | 27561675 |
| novel nad+-farnesal dehydrogenase from polygonum minus leaves. purification and characterization of enzyme in juvenile hormone iii biosynthetic pathway in plant. | juvenile hormone iii is of great concern due to negative effects on major developmental and reproductive maturation in insect pests. thus, the elucidation of enzymes involved jh iii biosynthetic pathway has become increasing important in recent years. one of the enzymes in the jh iii biosynthetic pathway that remains to be isolated and characterized is farnesal dehydrogenase, an enzyme responsible to catalyze the oxidation of farnesal into farnesoic acid. a novel nad+-farnesal dehydrogenase of p ... | 2016 | 27560927 |
| zika fetal neuropathogenesis: etiology of a viral syndrome. | the ongoing zika virus epidemic in the americas and the observed association with both fetal abnormalities (primary microcephaly) and adult autoimmune pathology (guillain-barré syndrome) has brought attention to this neglected pathogen. while initial case studies generated significant interest in the zika virus outbreak, larger prospective epidemiology and basic virology studies examining the mechanisms of zika viral infection and associated pathophysiology are only now starting to be published. ... | 2016 | 27560129 |
| evidence of the presence of the zika virus in mexico since early 2015. | to assess the possible circulation of zika virus (zikv) prior to the first documented case in mexico, we reanalyzed the stored samples from the states of veracruz and yucatán, which were originally collected to test for dengue (denv) and chikungunya (chikv) but were negative for these viruses despite the symptomatology. the samples were originally collected between the 30 and 46 epidemiological weeks (ew) when the zikv was not yet declared as a public health emergency of international concern (p ... | 2016 | 27557815 |
| synergistic and compensatory effects of two point mutations conferring target-site resistance to fipronil in the insect gaba receptor rdl. | insecticide resistance can arise from a variety of mechanisms, including changes to the target site, but is often associated with substantial fitness costs to insects. here we describe two resistance-associated target-site mutations that have synergistic and compensatory effects that combine to produce high and persistent levels of resistance to fipronil, an insecticide targeting on γ-aminobytyric acid (gaba) receptors. in nilaparvata lugens, a major pest of rice crops in many parts of asia, we ... | 2016 | 27557781 |
| culex species mosquitoes and zika virus. | recent reports of zika virus (zikv) isolates from culex species mosquitoes have resulted in concern regarding a lack of knowledge on the number of competent vector species for zikv transmission in the new world. although observations in the field have demonstrated that zikv isolation can be made from culex species mosquitoes, the detection of zikv in these mosquitoes is not proof of their involvement in a zikv transmission cycle. detection may be due to recent feeding on a viremic vertebrate, an ... | 2016 | 27556838 |
| the silencing of a 14-3-3ɛ homolog in tenebrio molitor leads to increased antimicrobial activity in hemocyte and reduces larval survivability. | the 14-3-3 family of phosphorylated serine-binding proteins acts as signaling molecules in biological processes such as metabolism, division, differentiation, autophagy, and apoptosis. herein, we report the requirement of 14-3-3ɛ isoform from tenebrio molitor (tm14-3-3ɛ) in the hemocyte antimicrobial activity. the tm14-3-3ɛ transcript is 771 nucleotides in length and encodes a polypeptide of 256 amino acid residues. the protein has the typical 14-3-3 domain, the nuclear export signal (nes) seque ... | 2016 | 27556493 |
| what's in the literature? | the guillain-barré syndrome (gbs) is one of the few neuropathies well known to the general public, in part because of its association with swine flu vaccinations in 1976. gbs has again reached the general public with its possible association with zika virus. the virus, borne by infected aedes aegypti mosquitos, is being linked to birth defects when pregnant women are bitten and infected. there are early reports also linking gbs to zika infection, which could expose a wider range of infected peop ... | 2016 | 27552392 |
| threat from emerging vectorborne viruses. | 0 | 27551712 | |
| development and utility of an in vitro, fluorescence-based assay for the discovery of novel compounds against dengue 2 viral protease. | dengue disease is one of the most significant vector-borne illnesses in the world. the emergence and re-emergence of dengue infections in many parts of the world affect millions annually and continue to burden public health systems especially in low-income populations. advances in dengue vaccine development showed promising results; however, protection seems to be suboptimal. there is no licensed chemotherapeutic agent against dengue to date. an ideal scenario of combinatorial vaccination of hig ... | 2016 | 27551237 |
| functional genomic analyses of enterobacter, anopheles and plasmodium reciprocal interactions that impact vector competence. | malaria exerts a tremendous socioeconomic impact worldwide despite current control efforts, and novel disease transmission-blocking strategies are urgently needed. the enterobacter bacterium esp_z, which is naturally harboured in the mosquito midgut, can inhibit the development of plasmodium parasites prior to their invasion of the midgut epithelium through a mechanism that involves oxidative stress. here, a multifaceted approach is used to study the tripartite interactions between the mosquito, ... | 2016 | 27549662 |
| spatiotemporal analysis of dengue fever in nepal from 2010 to 2014. | due to recent emergence, dengue is becoming one of the major public health problems in nepal. the numbers of reported dengue cases in general and the area with reported dengue cases are both continuously increasing in recent years. however, spatiotemporal patterns and clusters of dengue have not been investigated yet. this study aims to fill this gap by analyzing spatiotemporal patterns based on monthly surveillance data aggregated at district. | 2016 | 27549095 |
| advances and limitations of disease biogeography using ecological niche modeling. | mapping disease transmission risk is crucial in public and animal health for evidence based decision-making. ecology and epidemiology are highly related disciplines that may contribute to improvements in mapping disease, which can be used to answer health related questions. ecological niche modeling is increasingly used for understanding the biogeography of diseases in plants, animals, and humans. however, epidemiological applications of niche modeling approaches for disease mapping can fail to ... | 2016 | 27547199 |
| the viral capping enzyme nsp1: a novel target for the inhibition of chikungunya virus infection. | the chikungunya virus (chikv) has become a substantial global health threat due to its massive re-emergence, the considerable disease burden and the lack of vaccines or therapeutics. we discovered a novel class of small molecules ([1,2,3]triazolo[4,5-d]pyrimidin-7(6h)-ones) with potent in vitro activity against chikv isolates from different geographical regions. drug-resistant variants were selected and these carried a p34s substitution in non-structural protein 1 (nsp1), the main enzyme involve ... | 2016 | 27545976 |
| male tarsi specific odorant-binding proteins in the diving beetle cybister japonicus sharp. | odorant binding proteins (obps) play critical roles in chemical communication of insects, as they recognize and transport environmental chemical signals to receptors. the diving beetle cybister japonicus sharp shows a remarkable sexual dimorphism. the foreleg tarsi of males are equipped with large suction cups, believed to help holding the female during underwater courtship and mating. here, we identified two obps highly and specifically expressed in male tarsi, suggesting important functions of ... | 2016 | 27545810 |
| novel insights into insect-microbe interactions-role of epigenomics and small rnas. | it has become increasingly clear that microbes form close associations with the vast majority of animal species, especially insects. in fact, an array of diverse microbes is known to form shared metabolic pathways with their insect hosts. a growing area of research in insect-microbe interactions, notably for hemipteran insects and their mutualistic symbionts, is to elucidate the regulation of this inter-domain metabolism. this review examines two new emerging mechanisms of gene regulation and th ... | 2016 | 27540386 |
| complete genome sequences of three historically important, spatiotemporally distinct, and genetically divergent strains of zika virus: mr-766, p6-740, and prvabc-59. | here, we report the 10,807-nucleotide-long consensus rna genome sequences of three spatiotemporally distinct and genetically divergent zika virus strains, with the functionality of their genomic sequences substantiated by reverse genetics: mr-766 (african lineage, uganda, 1947), p6-740 (asian lineage, malaysia, 1966), and prvabc-59 (asian lineage-derived american strain, puerto rico, 2015). | 2016 | 27540058 |
| rural-urban comparisons of dengue seroprevalence in malaysia. | each year an estimated 390 million dengue infections occur worldwide. in malaysia, dengue is a growing public health concern but estimate of its disease burden remains uncertain. we compared the urban-rural difference of dengue seroprevalence and determined age-specific dengue seroprevalence in malaysia. | 2016 | 27538986 |
| seminal fluid protein genes of the brown planthopper, nilaparvata lugens. | seminal fluid proteins (sfps) are produced mainly in the accessory gland of male insects and transferred to females during mating, in which they induce numerous physiological and post-mating behavioral changes. the brown plant hopper (bph), nilaparvata lugens, is an economically important hemipterous pest of rice. the behavior and physiology of the female of this species is significantly altered by mating. sfps in hemipteran species are still unclear. | 2016 | 27538518 |
| genome-wide profiling of 24 hr diel rhythmicity in the water flea, daphnia pulex: network analysis reveals rhythmic gene expression and enhances functional gene annotation. | marine and freshwater zooplankton exhibit daily rhythmic patterns of behavior and physiology which may be regulated directly by the light:dark (ld) cycle and/or a molecular circadian clock. one of the best-studied zooplankton taxa, the freshwater crustacean daphnia, has a 24 h diel vertical migration (dvm) behavior whereby the organism travels up and down through the water column daily. dvm plays a critical role in resource tracking and the behavioral avoidance of predators and damaging ultravio ... | 2016 | 27538446 |
| the buzz about honey bee viruses. | 2016 | 27537076 | |
| resurgence of yellow fever in angola, 2015-2016. | 2016 | 27536787 | |
| dengue infection. | dengue is widespread throughout the tropics and local spatial variation in dengue virus transmission is strongly influenced by rainfall, temperature, urbanization and distribution of the principal mosquito vector aedes aegypti. currently, endemic dengue virus transmission is reported in the eastern mediterranean, american, south-east asian, western pacific and african regions, whereas sporadic local transmission has been reported in europe and the united states as the result of virus introductio ... | 2016 | 27534439 |
| eggs viability of aedes aegypti linnaeus (diptera, culicidae) under different environmental and storage conditions in manaus, amazonas, brazil. | the viability of aedes aegypti eggs was assessed in the amazon region. the eggs were maintained under different conditions: indoors (insectarium) and outdoors (natural environment), as well as in different storage types (plastic cup, paper envelope, plastic bag) for different days. egg viability was measured as the mean of hatchings observed from egg-bearing sheets of filter paper immersed in water, using three sheets randomly selected from each storage type and at both sites. there were signifi ... | 2016 | 27533732 |
| autochthonous chikungunya fever in traveler returning to japan from cuba. | 0 | 27533717 | |
| symptomatic dengue disease in five southeast asian countries: epidemiological evidence from a dengue vaccine trial. | dengue incidence has increased globally, but empirical burden estimates are scarce. prospective methods are best-able to capture all severities of disease. cyd14 was an observer-blinded dengue vaccine study conducted in children 2-14 years of age in indonesia, malaysia, thailand, the philippines, and vietnam. the control group received no vaccine and resembled a prospective, observational study. we calculated the rates of dengue according to different laboratory or clinical criteria to make infe ... | 2016 | 27532617 |
| multiple modes of action of the squamocin in the midgut cells of aedes aegypti larvae. | annonaceous acetogenins are botanical compounds with good potential for use as insecticides. in the vector, aedes aegypti (l.) (diptera: culicidae), squamocin (acetogenin) has been reported to be a larvicide and cytotoxic, but the modes of action of this molecule are still poorly understood. this study evaluated the changes in the cell morphology, and in the expression of genes, for autophagy (atg1 and atg8), for membrane ion transporter v-atpase, and for water channel aquaporin-4 (aqp4) in the ... | 2016 | 27532504 |
| design and testing of novel lethal ovitrap to reduce populations of aedes mosquitoes: community-based participatory research between industry, academia and communities in peru and thailand. | dengue virus (and chikungunya and zika viruses) is transmitted by aedes aegypti and aedes albopictus mosquitoes and causes considerable human morbidity and mortality. as there is currently no vaccine or chemoprophylaxis to protect people from dengue virus infection, vector control is the only viable option for disease prevention. the purpose of this paper is to illustrate the design and placement process for an attractive lethal ovitrap to reduce vector populations and to describe lessons learne ... | 2016 | 27532497 |
| modeling mosquito-borne disease spread in u.s. urbanized areas: the case of dengue in miami. | expansion of mosquito-borne pathogens into more temperate regions of the world necessitates tools such as mathematical models for understanding the factors that contribute to the introduction and emergence of a disease in populations naïve to the disease. often, these models are not developed and analyzed until after a pathogen is detected in a population. in this study, we develop a spatially explicit stochastic model parameterized with publicly available u.s. census data for studying the poten ... | 2016 | 27532496 |
| evolutionary potential of the extrinsic incubation period of dengue virus in aedes aegypti. | dengue fever is the most common arboviral disease worldwide. it is caused by dengue viruses (denv) and the mosquito aedes aegypti is its primary vector. one of the most powerful determinants of a mosquito's ability to transmit denv is the length of the extrinsic incubation period (eip), the time it takes for a virus to be transmitted by a mosquito after consuming an infected blood meal. here, we repeatedly measured denv load in the saliva of individual mosquitoes over their lifetime and used thi ... | 2016 | 27530960 |
| determination of juvenile hormone titers by means of lc-ms/ms/ms and a juvenile hormone-responsive gal4/uas system in aedes aegypti mosquitoes. | in anautogenous mosquitoes, juvenile hormone iii (jh) plays an essential role in female post-eclosion (pe) development, preparing them for subsequent blood feeding and egg growth. we re-examined the jh titer during the reproductive cycle of female aedes aegypti mosquitoes. using liquid chromatography coupled with triple tandem mass spectrometry (lc-ms/ms/ms), we have shown that it reaches its peak at 48-54 h pe in the female hemolymph and at 72 h pe in whole body extracts. this method represents ... | 2016 | 27530057 |
| aedes aegypti suppression in the americas: historical perspectives. | 2016 | 27529102 | |
| mosquito avoidance practices and knowledge of arboviral diseases in cities with differing recent history of disease. | as the range of dengue virus (denv) transmission expands, an understanding of community uptake of prevention and control strategies is needed both in geographic areas where the virus has recently been circulating and in areas with the potential for denv introduction. personal protective behaviors such as the use of mosquito repellent to limit human-vector contact and the reduction of vector density through elimination of oviposition sites are the primary control methods for aedes aegypti, the ma ... | 2016 | 27527634 |
| a crossover study to evaluate the diversion of malaria vectors in a community with incomplete coverage of spatial repellents in the kilombero valley, tanzania. | malaria elimination is unlikely to occur if vector control efforts focus entirely on transmission occurring indoors without addressing vectors that bite outdoors and outside sleeping hours. additional control tools such as spatial repellents may provide the personal protection required to fill this gap. however, since repellents do not kill mosquitoes it is unclear if vectors will be diverted from households that use spatial repellents to those that do not. | 2016 | 27527601 |
| phylogenetic analysis of the atp-binding cassette transporter family in three mosquito species. | the atp-binding cassette (abc) transporter family functions in the atp-dependent transportation of various substrates across biological membranes. abc proteins participate in various biological processes and insecticide resistance in insects, and are divided into eight subfamilies (a-h). mosquitoes are important vectors of human diseases, but the mechanism by which the abc transporter family evolves in mosquitoes is unknown. in this study, we classified and compared the abc transporter families ... | 2016 | 27521922 |
| nationwide study of factors associated with public's willingness to use home self-test kit for dengue fever in malaysia. | as there is no specific treatment for dengue, early detection and access to proper treatment may lower dengue fatality. therefore, having new techniques for the early detection of dengue fever, such as the use of dengue test kit, is vitally important. the aims of the study were: 1) identify factors associated with acceptance of a home self-test kit for dengue fever if the dengue test is available to the public and 2) find out the characteristics of the test kits that influence the use of the den ... | 2016 | 27520825 |
| the importance of male body size on sperm uptake and usage, and female fecundity in aedes aegypti and aedes albopictus. | adult mosquito density is a critical factor in the transmission of arboviruses by container aedes spp. mosquitoes. female fecundity drives population growth, and therefore contributes to adult mosquito density. previous studies have focused on female body size as the major determinant of fecundity, paying little attention to male condition. in this study, we examined the effects of male body size on the abundance of sperm in spermatheca, depletion of sperm over time, and female fecundity. | 2016 | 27519588 |
| promising new tools to fight aedes mosquitoes. | two new tools for suppressing aedes aegypti mosquito populations have been recommended for pilot testing. carefully designed trials will be needed to see whether they actually reduce disease as well. andréia azevedo soares reports. | 0 | 27516632 |
| public health round-up. | 0 | 27516631 | |
| impact of dengue virus (denv) co-infection on clinical manifestations, disease severity and laboratory parameters. | the co-circulation of 4 denv serotypes in geographically expanding area, has resulted in increasing occurrence of denv co-infections. however, studies assessing the clinical impact of denv co-infections have been scarce and have involved small number of patients. this study explores the impact of denv co-infection on clinical manifestations and laboratory parameters. | 2016 | 27514512 |
| traditional medicine practices among community members with diabetes mellitus in northern tanzania: an ethnomedical survey. | diabetes is a growing burden in sub-saharan africa where traditional medicines (tms) remain a primary form of healthcare in many settings. in tanzania, tms are frequently used to treat non-communicable diseases, yet little is known about tm practices for non-communicable diseases like diabetes. | 2016 | 27514380 |
| recent chikungunya virus infection in 2 travelers returning from mogadishu, somalia, to italy, 2016. | 2016 | 27513985 | |
| leading indicators of mosquito-borne disease elimination. | mosquito-borne diseases contribute significantly to the global disease burden. high-profile elimination campaigns are currently underway for many parasites, e.g., plasmodium spp., the causal agent of malaria. sustaining momentum near the end of elimination programs is often difficult to achieve and consequently quantitative tools that enable monitoring the effectiveness of elimination activities after the initial reduction of cases has occurred are needed. documenting progress in vector-borne di ... | 2015 | 27512522 |
| a community-based prospective cohort study of dengue viral infection in malaysia: the study protocol. | globally, dengue infections constitute a significant public health burden. in recent decades, malaysia has become a dengue hyper-endemic country with the co-circulation of the four dengue virus serotypes. the cyclical dominance of sub-types contributes to a pattern of major outbreaks. the consequences can be observed in the rising incidence of reported dengue cases and dengue related deaths. understanding the complex interaction of the dengue virus, its human hosts and the mosquito vectors at th ... | 2016 | 27510731 |
| extended evaluation of virological, immunological and pharmacokinetic endpoints of celaden: a randomized, placebo-controlled trial of celgosivir in dengue fever patients. | celaden was a randomized placebo-controlled trial of 50 patients with confirmed dengue fever to evaluate the efficacy and safety of celgosivir (a study registered at clinicaltrials.gov, number nct01619969). celgosivir was given as a 400 mg loading dose and 200 mg bid (twice a day) over 5 days. replication competent virus was measured by plaque assay and compared to reverse transcription quantitative pcr (qpcr) of viral rna. pharmacokinetics (pk) correlations with viremia, immunological profiling ... | 2016 | 27509020 |
| solution nuclear magnetic resonance studies of sterol carrier protein 2 like 2 (scp2l2) reveal the insecticide specific structural characteristics of scp2 proteins in aedes aegypti mosquitoes. | sterol carrier protein 2 like 2 from aedes aegypti (aescp2l2) plays an important role in lipid transport in mosquitoes for its routine metabolic processes. repeated unsuccessful attempts to crystallize ligand free scp2l2 prompted us to undertake nuclear magnetic resonance (nmr) spectroscopy to determine its three-dimensional structure. we report here the three-dimensional structures and dynamics of apo-aescp2l2 and its complex with palmitate. the (15)n heteronuclear single-quantum coherence spec ... | 2016 | 27508310 |
| external quality assessment of dengue and chikungunya diagnostics in the asia pacific region, 2015. | to conduct an external quality assessment (eqa) of dengue and chikungunya diagnostics among national-level public health laboratories in the asia pacific region following the first round of eqa for dengue diagnostics in 2013. | 2016 | 27508088 |
| symbiosis with francisella tularensis provides resistance to pathogens in the silkworm. | francisella tularensis, the causative agent of tularemia, is a highly virulent facultative intracellular pathogen found in a wide range of animals, including arthropods, and environments. this bacterium has been known for over 100 years, but the lifestyle of f. tularensis in natural reservoirs remains largely unknown. thus, we established a novel natural host model for f. tularensis using the silkworm (bombyx mori), which is an insect model for infection by pathogens. f. tularensis established a ... | 2016 | 27507264 |
| conidiobolus macrosporus (entomophthorales), a mosquito pathogen in central brazil. | a new fungal pathogen of culicinae (diptera: culicidae) adults, conidiobolus macrosporus (entomophthorales: ancylistaceae), was detected and isolated during a survey of mosquito pathogens close to the city of aruanã, goiás state, in december 2014. the morphological characteristics of c. macrosporus are presented, and reasons for some uncertainty about this identification are discussed. the pathogenicity and high virulence of this fungus for aedes aegypti were confirmed in laboratory conditions. ... | 2016 | 27506454 |
| zika virus, a new threat for europe? | since its emergence in 2007 in micronesia and polynesia, the arthropod-borne flavivirus zika virus (zikv) has spread in the americas and the caribbean, following first detection in brazil in may 2015. the risk of zikv emergence in europe increases as imported cases are repeatedly reported. together with chikungunya virus (chikv) and dengue virus (denv), zikv is transmitted by aedes mosquitoes. any countries where these mosquitoes are present could be potential sites for future zikv outbreak. we ... | 2016 | 27505002 |
| computational characterization of iron metabolism in the tsetse disease vector, glossina morsitans: ire stem-loops. | iron metabolism and regulation is an indispensable part of species survival, most importantly for blood feeding insects. iron regulatory proteins are central regulators of iron homeostasis, whose binding to iron response element (ire) stem-loop structures within the utrs of genes regulate expression at the post-transcriptional level. despite the extensive literature on the mechanism of iron regulation in human, less attention has been given to insect and more specifically the blood feeding insec ... | 2016 | 27503259 |
| systemically and cutaneously distributed ectoparasiticides: a review of the efficacy against ticks and fleas on dogs. | acaricidal (tick) and insecticidal (flea) efficacy of systemically and cutaneously distributed ectoparasiticide products for dogs are compared based on permethrin and fluralaner as representative molecules. results of efficacy studies against fleas and ticks are reviewed that show generally good to excellent results. both externally and systemically distributed treatments have benefits and weaknesses in potentially preventing pathogen transmission by these arthropod vectors.four general properti ... | 2016 | 27502490 |
| identification of aacasps7, an effector caspase in aedes albopictus. | aedes albopictus mosquito is a vector of various arboviruses and is becoming a significant threat to public health due to its rapid global expansion. several reports suggest that apoptosis could be a factor limiting arbovirus infection in mosquitoes. thus, it is significant to identify apoptosis pathway and study the correlation between apoptosis and virus infection in mosquitoes. apoptosis is a type of programmed cell death that plays a vital role in immunity, development, and tissue homeostasi ... | 2016 | 27502418 |
| larvicidal activity of natural and modified triterpenoids against aedes aegypti (diptera: culicidae). | insecticide resistance to commonly used substances demands new molecules for the chemical control of the dengue vector aedes aegypti. because natural product sources have been an alternative to obtain larvicidal compounds, the aim of this study was to evaluate the triterpenoids betulinic (ba) and ursolic (ua) acids and their semi-synthetic derivatives against larval ae. aegypti. ba, ua, ten derivatives modified at the c-3 position and a positive control (diflubenzuron) were evaluated. larvicidal ... | 2016 | 27501778 |
| the critical role of early dengue surveillance and limitations of clinical reporting - implications for non-endemic countries. | the increasing dengue burden and epidemic severity worldwide have highlighted the need to improve surveillance. in non-endemic areas such as taiwan, where outbreaks start mostly with imported cases from southeast asia, a closer examination of surveillance dynamics to detect cases early is necessary. to evaluate problems with dengue surveillance and investigate the involvement of different factors at various epidemic stages, we investigated 632 laboratory-confirmed indigenous dengue cases in kaoh ... | 2016 | 27501302 |
| calculation of the average cost per case of dengue fever in mexico using a micro-costing approach. | the increasing burden of dengue fever (df) in the americas, and the current epidemic in previously unaffected countries, generate major costs for national healthcare systems. there is a need to quantify the average cost per df case. in mexico, few data are available on costs, despite df being endemic in some areas. extrapolations from studies in other countries may prove unreliable and are complicated by the two main mexican healthcare systems (the secretariat of health [ss] and the mexican soci ... | 2016 | 27501146 |
| rise and fall of vector infectivity during sequential strain displacements by mosquito-borne dengue virus. | each of the four serotypes of mosquito-borne dengue virus (denv-1-4) comprises multiple, genetically distinct strains. competitive displacement between strains within a serotype is a common feature of denv epidemiology and can trigger outbreaks of dengue disease. we investigated the mechanisms underlying two sequential displacements by denv-3 strains in sri lanka that each coincided with abrupt increases in dengue haemorrhagic fever (dhf) incidence. first, the post-dhf strain displaced the pre-d ... | 2016 | 27500505 |
| persistence of neutralizing antibody against dengue virus 2 after 70 years from infection in nagasaki. | this study aimed to investigate the duration of humoral immune responses to dengue virus (denv) infection in japanese who experienced acute febrile illness with hemorrhagic manifestations 70 years ago, when an epidemic of dengue occurred in nagasaki, japan, from 1942 to 1944. a japanese volunteer requested serological diagnosis of denv infection in 2014 and donated blood sample to measure the antibody titer against denv by antiflavi igg indirect elisa, focus reduction neutralization test, and pl ... | 2016 | 27493841 |
| coexistence of aedes aegypti and aedes albopictus (diptera: culicidae) in peninsular florida two decades after competitive displacements. | the spread of aedes albopictus (skuse) eastward in the mid-1980s from its initial establishment in houston, tx, was associated with rapid declines and local disappearances of aedes aegypti (l.) in gulf coast states and florida where annual larval surveillance during the early 1990s described temporal and spatial patterns of competitive displacements in cemeteries and tire shops. approximately 20 yr later in 2013-2014, we re-visited former collection sites and sampled aquatic immatures of these t ... | 2016 | 27493253 |
| knockdown resistance mutations in aedes aegypti (diptera: culicidae) from puerto rico. | permethrin resistance is widespread in aedes aegypti (l.), the main dengue, zika, and chikungunya virus vector in latin america and the caribbean. a common mechanism of resistance to pyrethroids-knockdown resistance (kdr)-is conferred through mutations in the insect's voltage-dependent sodium channel. in this mosquito, around 10 replacement substitutions in the voltage-gated sodium channel gene (vgsc) have been reported in pyrethroid-resistant strains. two of these mutations, named ile1,016 and ... | 2016 | 27493252 |
| one-pot biogenic fabrication of silver nanocrystals using quisqualis indica: effectiveness on malaria and zika virus mosquito vectors, and impact on non-target aquatic organisms. | currently, mosquito vector control is facing a number of key challenges, including the rapid development of resistance to synthetic pesticides and the recent spread of aggressive arbovirus outbreaks. the biosynthesis of silver nanoparticles (agnps) is currently considered an environmental friendly alternative to the employ of pyrethroids, carbamates and microbial agents (e.g. bacillus thuringiensis var. israelensis), since agnps are easy to produce, effective and stable in the aquatic environmen ... | 2016 | 27491031 |
| contraceptive use among nonpregnant and postpartum women at risk for unintended pregnancy, and female high school students, in the context of zika preparedness - united states, 2011-2013 and 2015. | zika virus infection during pregnancy can cause congenital microcephaly and brain abnormalities (1,2). since 2015, zika virus has been spreading through much of the world health organization's region of the americas, including u.s. territories. zika virus is spread through the bite of aedes aegypti or aedes albopictus mosquitoes, by sex with an infected partner, or from a pregnant woman to her fetus during pregnancy.* cdc estimates that 41 states are in the potential range of aedes aegypti or ae ... | 2016 | 27490117 |
| update: ongoing zika virus transmission - puerto rico, november 1, 2015-july 7, 2016. | zika virus is a flavivirus transmitted primarily by aedes aegypti and aedes albopictus mosquitoes, and infection can be asymptomatic or result in an acute febrile illness with rash (1). zika virus infection during pregnancy is a cause of microcephaly and other severe birth defects (2). infection has also been associated with guillain-barré syndrome (gbs) (3) and severe thrombocytopenia (4,5). in december 2015, the puerto rico department of health (prdh) reported the first locally acquired case o ... | 2016 | 27490087 |
| prospects for dengue vaccines for travelers. | travel-acquired dengue cases have been increasing as the overall global dengue burden has expanded. in korea, imported dengue cases have been reported since 2000 when it first became a notifiable disease. during the first four months of 2016, three times more dengue cases were reported in korea than during the same period the previous year. a safe and efficacious vaccine for travelers would be beneficial to prevent dengue disease in individual travelers and potentially decrease the risk of virus ... | 2016 | 27489798 |
| microrna-309 targets the homeobox gene six4 and controls ovarian development in the mosquito aedes aegypti. | obligatory blood-triggered reproductive strategy is an evolutionary adaptation of mosquitoes for rapid egg development. it contributes to the vectorial capacity of these insects. therefore, understanding the molecular mechanisms underlying reproductive processes is of particular importance. here, we report that microrna-309 (mir-309) plays a critical role in mosquito reproduction. a spatiotemporal expression profile of mir-309 displayed its blood feeding-dependent onset and ovary-specific manife ... | 2016 | 27489347 |
| racing against time against zika. | 0 | 27489331 | |
| zika virus: indian perspectives. | the emergence of zika virus (ziv), a mosquito borne flavivirus like dengue (den) and chikungunya (chik), in brazil in 2014 and its spread to various countries have led to a global health emergency. aedes aegypti is the major vector for ziv. fast dissemination of this virus in different geographical areas posses a major threat especially to regions where the population lacks herd immunity against the ziv and there is abundance of aedes mosquitoes. in this review, we focus on current global scenar ... | 0 | 27487998 |
| tourniquet test for dengue diagnosis: systematic review and meta-analysis of diagnostic test accuracy. | dengue fever is a ubiquitous arboviral infection in tropical and sub-tropical regions, whose incidence has increased over recent decades. in the absence of a rapid point of care test, the clinical diagnosis of dengue is complex. the world health organisation has outlined diagnostic criteria for making the diagnosis of dengue infection, which includes the use of the tourniquet test (tt). | 2016 | 27486661 |
| the sex locus is tightly linked to factors conferring sex-specific lethal effects in the mosquito aedes aegypti. | in many taxa, sex chromosomes are heteromorphic and largely non-recombining. evolutionary models predict that spread of recombination suppression on the y chromosome is fueled by the accumulation of sexually antagonistic alleles in close linkage to the sex determination region. however, empirical evidence for the existence of sexually antagonistic alleles is scarce. in the mosquito aedes aegypti, the sex-determining chromosomes are homomorphic. the region of suppressed recombination, which surro ... | 2016 | 27485667 |
| genome-wide identification and characterization of carboxypeptidase genes in silkworm (bombyx mori). | the silkworm (bombyx mori) is an economically-important insect that can secrete silk. carboxypeptidases have been found in various metazoan species and play important roles in physiological and biochemical reactions. here, we analyzed the silkworm genome database and characterized 48 carboxypeptidases, including 34 metal carboxypeptidases (bmmcp1-bmmcp34) and 14 serine carboxypeptidases (bmscp1-bmscp14), to better understand their diverse functions. compared to other insects, our results indicat ... | 2016 | 27483237 |
| species-specific escape of plasmodium sporozoites from oocysts of avian, rodent, and human malarial parasites. | malaria is transmitted when an infected mosquito delivers plasmodium sporozoites into a vertebrate host. there are many species of plasmodium and, in general, the infection is host-specific. for example, plasmodium gallinaceum is an avian parasite, while plasmodium berghei infects mice. these two parasites have been extensively used as experimental models of malaria transmission. plasmodium falciparum and plasmodium vivax are the most important agents of human malaria, a life-threatening disease ... | 2016 | 27480269 |
| yellow fever-more a policy and planning problem than a biological one. | 2016 | 27479749 | |
| using wolbachia releases to estimate aedes aegypti (diptera: culicidae) population size and survival. | mosquitoes carrying the endosymbiont bacterium wolbachia have been deployed in field trials as a biological control intervention due to wolbachia effects on reducing transmission of arboviruses. we performed mark, release and recapture (mrr) experiments using wolbachia as an internal marker with daily collections with bg-traps during the first two weeks of releases in rio de janeiro, brazil. the mrr design allowed us to investigate two critical parameters that determine whether wolbachia would s ... | 2016 | 27479050 |
| molecular characterization of an adiponectin receptor homolog in the white leg shrimp, litopenaeus vannamei. | adiponectin (adipoq) and its receptors (adipors) are strongly related to growth and development of skeletal muscle, as well as glucose and lipid metabolism in vertebrates. herein we report the identification of the first full-length cdna encoding an adipor homolog (liv-adipor) from the decapod crustacean litopenaeus vannamei using a combination of next generation sequencing (ngs) technology and bioinformatics analysis. the full-length liv-adipor (1,245 bp) encoded a protein that exhibited the ca ... | 2016 | 27478708 |
| artisanal extraction and traditional knowledge associated with medicinal use of crabwood oil (carapa guianensis aublet.) in a peri-urban várzea environment in the amazon estuary. | várzea forests of the amazon estuary contain species of importance to riverine communities. for example, the oil extracted from the seeds of crabwood trees is traditionally used to combat various illnesses and as such artisanal extraction processes have been maintained. the objectives of this study were to (1) describe the process involved in artisanal extraction of crabwood oil in the fazendinha protected area, in the state of amapá; (2) characterise the processes of knowledge transfer associat ... | 2016 | 27478479 |
| potential for extrinsic incubation temperature to alter interplay between transmission potential and mortality of dengue-infected aedes aegypti. | the extrinsic incubation period is a critical component in the assessment of arboviral transmission potential. it defines the time it takes for a mosquito to become infectious following exposure to an arbovirus. since this is a temporal process, the lifespan of a mosquito is intimately tied to the extrinsic incubation period and thus transmission potential of these viruses. temperature is a known effector of both vector competence (the ability of a vector to transmit a pathogen) and mosquito mor ... | 2016 | 27478382 |