Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| designing recombinant pseudomonas strains to enhance biodesulfurization. | the dsz biodesulfurization cluster from rhodococcus erythropolis igts8 has been engineered under the control of heterologous broad-host-range regulatory signals to alleviate the mechanism of sulfur repression, and it was stably inserted into the chromosomes of different pseudomonas strains. the recombinant bacteria were able to desulfurize dibenzothiophene more efficiently than the native host. furthermore, these new biocatalysts combine relevant industrial and environmental traits, such as prod ... | 1997 | 9371464 |
| structure of the photoreactive iron center of the nitrile hydratase from rhodococcus sp. n-771. evidence of a novel post-translational modification in the cysteine ligand. | nitrile hydratase (nhase) from rhodococcus sp. n-771 is a photoreactive enzyme that is inactivated by nitrosylation of the non-heme iron center and activated by photodissociation of nitric oxide (no). to obtain structural information on the iron center, we isolated peptide complexes containing the iron center by proteolysis. when the tryptic digest of the alpha subunit isolated from the inactive form was analyzed by reversed-phase high performance liquid chromatography, the absorbance characteri ... | 1997 | 9368004 |
| identification and characterization of an aliphatic amidase in helicobacter pylori. | we report, for the first time, the presence in helicobacter pylori of an aliphatic amidase that, like urease, contributes to ammonia production. aliphatic amidases are cytoplasmic acylamide amidohydrolases (ec 3.5.1.4) hydrolysing short-chain aliphatic amides to produce ammonia and the corresponding organic acid. the finding of an aliphatic amidase in h. pylori was unexpected as this enzyme has only previously been described in bacteria of environmental (soil or water) origin. the h. pylori amid ... | 1997 | 9364923 |
| nucleic acid amplification for rapid detection of rhodococcus equi in equine blood and tracheal wash fluids. | to evaluate the ability of nucleic acid amplification techniques to detect rhodococcus equi in equine buffy coat, blood, and tracheal wash fluid and to differentiate between virulent and avirulent strains of the bacteria. | 1997 | 9361884 |
| biodegradation of the gasoline oxygenates methyl tert-butyl ether, ethyl tert-butyl ether, and tert-amyl methyl ether by propane-oxidizing bacteria. | several propane-oxidizing bacteria were tested for their ability to degrade gasoline oxygenates, including methyl tert-butyl ether (mtbe), ethyl tert-butyl ether (etbe), and tert-amyl methyl ether (tame). both a laboratory strain and natural isolates were able to degrade each compound after growth on propane. when propane-grown strain env425 was incubated with 20 mg of uniformly labeled [14c]mtbe per liter, the strain converted > 60% of the added mtbe to 14co2 in < 30 h. the initial oxidation of ... | 1997 | 9361407 |
| site-specific endonucleases rsplki and rsplkii from rhodococcus species lk2 are isoschizomers of sphi and bamhi. | two site-specific endonucleases, rsplki and rsplkii, have been isolated and purified to functional homogeneity from the soil bacterium rhodococcus species lk2. rsplki recognizes the 5'-gcatg decreases c-3' dna sequence and rsplkii recognizes the 5'-g decreases gatcc-3' sequence (arrows indicate dna cleavage sites). the isolated enzymes are class ii site specific endonucleases and are isoschizomers of endonucleases sphi and bamhi, respectively. | 1997 | 9360299 |
| microsporidium species in pulmonary cavitary lesions of aids patients infected with rhodococcus equi. | 1997 | 9356815 | |
| structural analysis of the 6 kb cryptic plasmid pfaj2600 from rhodococcus erythropolis ni86/21 and construction of escherichia coli-rhodococcus shuttle vectors. | the complete nucleotide sequence of the 5936 bp cryptic plasmid pfaj2600 from rhodococcus erythropolis ni86/21 was determined. based on the characteristics of its putative replication genes, repa and repb, pfaj2600 was assigned to the family of pal5000-related small replicons identified in mycobacterium (pal5000), corynebacterium (pxz10142), brevibacterium (prbl1), bifidobacterium (pmb1) and neisseria (pjd1). the replication systems of these plasmids show striking similarities to the ones used b ... | 1997 | 9353918 |
| rapid physiological characterization of microorganisms by biosensor technique. | eleven microorganisms, arxula adeninivorans ls3, candida boidinii dsm 70034, candida lactis-condensi dsm 70635, pichia jadinii dsm 2361, pichia minuta dsm 7018, kluyveromyces lactis dsm 4394, pseudomonas putida dsm 50026, alcaligenes sp. dsm 30002, arthrobacter nicotianae dsm 20123 as well as issatchenkia orientalis dsm 70077 and rhodococcus erythropolis dsm 311 were characterized by the sensor technique by injection of 30 different substrates and substrate mixtures. the obtained data which are ... | 1997 | 9352658 |
| purulent meningitis due to rhodococcus equi. a case of posttraumatic infection. | opportunistic infections due to rhodococcus equi have been increasingly reported in the immunocompromised population, especially in patients with aids. in this report, we present an unusual case of purulent meningitis that developed in an immunocompetent six-year-old child through direct inoculation of r. equi. | 1997 | 9350214 |
| identification of active sites in amidase: evolutionary relationship between amide bond- and peptide bond-cleaving enzymes. | mainly based on various inhibitor studies previously performed, amidases came to be regarded as sulfhydryl enzymes. not completely satisfied with this generally accepted interpretation, we performed a series of site-directed mutagenesis studies on one particular amidase of rhodococcus rhodochrous j1 that was involved in its nitrile metabolism. for these experiments, the recombinant amidase was produced as the inclusion body in escherichia coli to greatly facilitate its recovery and subsequent pu ... | 1997 | 9342349 |
| biodegradation of groundwater pollutants by a combined culture of mycobacterium vaccae and a rhodococcus sp. | the catabolism of selected groundwater pollutants by a combined culture of mycobacterium vaccae and a rhodococcus sp. (strain r-22) was investigated. the m. vaccae-r-22 combined culture was five times more effective in mineralizing benzene than either organism alone. mycobacterium vaccae oxidized benzene to phenol, and r-22 catabolized the phenol to cellular components and co2. benzene did not support growth of m. vaccae, r-22, or the combined culture. optimization of ratios of the two species i ... | 1997 | 9336947 |
| active site-directed inhibitors of rhodococcus 20 s proteasome. kinetics and mechanism. | we have studied the mechanism of inhibition of the recombinant rhodococcus proteasome by four different chemical classes of active site-directed small molecule inhibitors. clasto-lactacystin beta-lactone is a time-dependent inhibitor of the rhodococcus proteasome's ability to hydrolyze suc-leu-leu-val-tyr-amc, a substrate for this proteasome's single type of active site, and proceeds with a kinact/[i] of 1,700 m-1 s-1. using peptide mapping of tryptic digests, lc/ms, and amino acid sequence anal ... | 1997 | 9334174 |
| a 3-(3-hydroxyphenyl)propionic acid catabolic pathway in rhodococcus globerulus pwd1: cloning and characterization of the hpp operon. | rhodococcus globerulus pwd1, a soil isolate from a polluted site in the netherlands, is able to degrade a broad range of aromatic compounds. a novel gene cluster which appears to encode a pathway for the degradation of phenolic acids such as 3-(3-hydroxyphenyl)propionate (3hpp) has been cloned from the chromosome of this organism. sequence analysis of a 7-kb region identified five open reading frames (orfs). analysis of mrna showed that the genes were expressed during growth on 3hpp and 3-hydrox ... | 1997 | 9324265 |
| a rhodococcus species that thrives on medium saturated with liquid benzene. | a bacterium isolated from a contaminated site in sydney, australia, utilized benzene in the liquid phase as a sole carbon source at levels toxic to other micro-organisms. the organism was a short gram-positive rod which grew at 6% nacl, 0-37 degrees c and ph 2-10. biochemical tests, fatty acid analysis, and 16s rdna sequencing identified the organism as a member of the genus rhodococcus. vapour-phase addition of benzene to the medium in batch and continuous systems resulted in initial concentrat ... | 1997 | 9308180 |
| elucidation of the metabolic pathway for dibenzothiophene desulphurization by rhodococcus sp. strain igts8 (atcc 53968). | rhodococcus sp. strain igts8 (atcc 53968) is able to utilize dibenzothiophene (dbt) as a sole source of sulphur. the carbon skeleton of dbt is not metabolized and is conserved as 2-hydroxybiphenyl (hbp), which accumulates in the medium. this phenotype is due to the expression of the plasmid-encoded dbt-desulphurization (dsz) operon, which encodes three proteins, dsza, b and c. in this paper it is shown, using [35s]dbt radiolabelling studies, that sulphur is released in the form of inorganic sulp ... | 1997 | 9308179 |
| sepsis from rhodococcus equi successfully treated in a kidney transplant recipient. | 1997 | 9306362 | |
| biosynthesis of a cyclic tautomer of (3-methylmaleyl)acetone from 4-hydroxy-3,5-dimethylbenzoate by pseudomonas sp. hh35 but not by rhodococcus rhodochrous n75. | here we report that the bacterial catabolism of 4-hydroxy-3,5-dimethylbenzoic acid 1 takes a different course in rhodococcus rhodochrous n75 and pseudomonas sp. strain hh35. the former organism accumulates a degradation metabolite of the acid which we isolated and identified as 2,6-dimethylhydroquinone 2. the latter bacterial strain converts the acid and the hydroquinone into a dead-end metabolite. this novel compound was characterised unequivocally by mass spectrometry and 1h and 18c nmr and uv ... | 1997 | 9299478 |
| identification and characterization of is-like elements in mycobacterium gordonae. | a new insertion sequence, is1512, from mycobacterium gordonae was cloned and sequenced. this element is present in up to 10 copies which provides a high diversity for restriction fragment length polymorphism analysis. we have also identified truncated is1512-like elements, including a truncated is1512 and another truncated insertion sequence which displays homology with is1512 and was designated is1511. sequences homologous to the previously described transposon tn554 are inserted into these tru ... | 1997 | 9297826 |
| recalcitrance of 1,1-dichloro-2,2-bis(p-chlorophenyl)ethylene (dde) to cometabolic degradation by pure cultures of aerobic and anaerobic bacteria. | pure cultures of aerobic and anaerobic bacteria capable of oxidation and reductive dehalogenation of chloroethylenes, and aerobic bacteria involved in biodegradation of polychlorinated biphenyls (pcbs) were screened for their ability to cometabolize the persistent pollutant 1,1-dichloro-2,2-bis(p-chlorophenyl)ethylene (dde). bacterial cultures expressing methane monooxygenase (methylosinus trichosporium), propane monooxygenase (mycobacterium vaccae) and biphenyl 2,3-dioxygenase enzymes (pseudomo ... | 1997 | 9294241 |
| purification and characterization of limonoate dehydrogenase from rhodococcus fascians. | limonoate dehydrogenase from rhodococcus fascians has been purified to electrophoretic homogeneity by a procedure that consists of ion-exchange, hydrophobic, and affinity chromatography. the native enzyme has a molecular mass of around 128,000 da and appears to be composed of four similar subunits (30,000 da each). the isoelectric point is 4.9 as determined by isoelectric focusing. the homogeneous enzyme was used to determine the nh2-terminal amino acid sequence. the enzyme was purified from cel ... | 1997 | 9292989 |
| a novel approach to preventing insect-borne diseases. | 1997 | 9287238 | |
| cryptic plasmid pka22 isolated from the naphthalene degrading derivative of rhodococcus rhodochrous ncimb13064. | cryptic plasmids were found in rhodococcus rhodochrous ncimb13064 derivatives which had lost the ability to utilize short-chain 1-chloroalkanes (chain length c3-c10) and had acquired the ability to degrade naphthalene. the reversions of these derivatives to the original phenotype were accompanied by the loss of the cryptic plasmids. the 4969-bp pka22 plasmid was cloned in escherichia coli and sequenced. this plasmid encodes a putative 33,200-da protein which contains motifs typical of theta repl ... | 1997 | 9281496 |
| improvement of lysine production by analog-sensitive and auxotroph mutants of the acetylene-utilizing bacterium gordona bronchialis (rhodococcus bronchialis). | an acetylene utilizing gordona (rhodococcus) bronchialis strain, screened for the production of fine chemicals, was found to be capable of producing small amounts of lysine. attempts to produce amino-acid analog-resistant and/or sensitive mutants and auxotrophs of this strain with increased lysine production were made following uv-irradiation or n-methyl-n'-nitro-n-nitrosoguanidine (mnng) treatment. the bacterium exhibited surprisingly high resistance levels to the aforementioned mutagens which ... | 1997 | 9276926 |
| dense rhodococcus cerebral abscesses in an hiv-positive patient. | rhodococcus equi, formerly known as corynebacterium equi, an aerobic, gram-positive, pleomorphic coccobacillus, is a well-known pathogen for domestic livestock. we present a biopsy- and culture-proven case of rhodococcus equi brain abscesses in a patient seropositive for hiv, having an appearance not described previously. | 1997 | 9272496 |
| studies on the rod-coccus life cycle of rhodococcus equi. | in the present study all 19 rhodococcus equi cultures isolated from horses and 19 of 22 r. equi cultures isolated from human patients displayed a rod-coccus life cycle after cultivation under defined growth conditions. a bacillary growth could be observed after cultivation of the bacteria in fluid media for 4 h at 37 degrees c, a coccoid morphology after cultivation of the bacteria for 24 h either on sheep blood agar plates or in fluid media. the different morphological features did not signific ... | 1997 | 9270351 |
| [rhodococcus equi--a newly recognized opportunistic pathogen in man]. | the review informs about substantial features of rhodococcus equi with emphasis on the analysis of 115 as yet published and still expanding reports on the isolation of this zoopathogenic nocardioform actinomycete from man. microbiological laboratories of human medicine have to learn not only how to identify r. equi but also recognize it as an opportunistic pathogen in particular in persons with the deficient immunity. r. equi is a gram-positive, encapsulated diphtheroid coccobacillus, partially ... | 1997 | 9264795 |
| some biochemical properties and the classification of a range of bacterial haloalkane dehalogenases. | multivariate analyses and experimental data have been used to evaluate the relationships between eight bacterial hydrolytic haloalkane dehalogenases. the results indicate that seven of the dehalogenases investigated can confidently be placed into two classes [sensu slater, bull and hardman (1995) biodegradation 6, 181-189] according to their substrate profiles. the remaining enzyme, isolated from rhodococcus erythropolis cp9, appears to represent a third class of haloalkane dehalogenases. | 1997 | 9261999 |
| spontaneous resolution of rhodococcal pulmonary infection in a liver transplant recipient. | pulmonary infection by rhodococcus equi is characterized by indolent infection in an immunocompromised host with a propensity to form cavitary lesions. mortality can be greater than 50%; treatment involves prolonged therapy with multiple antibiotics and, occasionally, surgical resection. recurrence is common. we report a case of a liver transplant patient with a pulmonary nodule caused by r equi; the nodule followed a benign clinical course and resolved spontaneously. this case illustrates that ... | 1997 | 9258318 |
| further sequence analysis of the dna regions with the rhodococcus 20s proteasome structural genes reveals extensive homology with mycobacterium leprae. | the sequence of the respective dna regions downstream of the 20s proteasome structural genes prcb1a1 (6 kb) and prcb2a2 (3.3 kb) of rhodococcus erythropolis ni86/21 were determined. a highly conserved gene organization was observed between the two clusters which differed significantly in g + c content (68.8% versus 62.6%). several orfs were homologues of putative genes previously identified by genomic sequencing of the equivalent dna in the related nocardioform actinomycete, mycobacterium leprae ... | 1997 | 9254018 |
| three of the seven bphc genes of rhodococcus erythropolis ta421, isolated from a termite ecosystem, are located on an indigenous plasmid associated with biphenyl degradation. | rhodococcus erythropolis ta421, a polychlorinated biphenyl and biphenyl degrader isolated from a termite ecosystem, has seven bphc genes expressing 2,3-dihydroxybiphenyl dioxygenase activity. r. erythropolis ta421 harbored a large and probably linear plasmid on which three (bphc2, bphc3, and bphc4) of the seven bphc genes were located. a non-biphenyl-degrading mutant, designated strain ta422, was obtained spontaneously from r. erythropolis ta421. ta422 lacked the plasmid, suggesting that the thr ... | 1997 | 9251216 |
| genetic identification of members of the genus corynebacterium at genus and species levels with 16s rdna-targeted probes. | 16s rrna gene-targeted probes were designed for the identification of corynebacteria at the genus and species levels. the genus-specific probe hybridized all clinically important members of the genus corynebacterium and could distinguish them from other coryneform bacteria and phylogenetically related high g+c% gram-positive bacteria, including actinomyces, rhodococcus, gordona, nocardia, streptomyces, brevibacterium and mycobacterium. the species-specific probes for c. jeikeium and c. diphtheri ... | 1997 | 9251056 |
| new method for rna isolation from actinomycetes: application to the transcriptional analysis of the alcohol oxidoreductase gene thce in rhodococcus and mycobacterium. | a new protocol for the isolation of rna from rhodococcus and other actinomycetes such as mycobacterium and amycolatopsis was developed. the method is based on rapid lysis of cells in a high-speed reciprocal shaker using small abrasive particles followed by spin column purification of the lysate. this quick procedure yields rna preparations suitable for functional studies. this was shown for the thce gene of r. erythropolis ni86/21, which encodes a n,n'-dimethyl-4-nitrosoaniline-dependent alcohol ... | 1997 | 9248086 |
| molecular cloning, expression, and sequence analysis of the endoglycoceramidase ii gene from rhodococcus species strain m-777. | endoglycoceramidase (egcase (ec 3.2.1.123)) is a hydrolase that hydrolyzes the linkage between the oligosaccharide and ceramide of various glycosphingolipids. this paper describes the molecular cloning and expression of egcase ii, one of the isoforms of egcases. the gene encoding egcase ii was obtained by screening of a genomic dna library from rhodococcus sp. strain m-777 constructed in puc19 with oligonucleotide probes deduced from a partial amino acid sequence of the enzyme protein. recombina ... | 1997 | 9242646 |
| reclassification of nocardioides simplex atcc 13260, atcc 19565, and atcc 19566 as rhodococcus erythropolis. | our phylogenetic analysis based on 16s ribosomal dna (rdna) sequences and chemotaxonomic analyses showed that nocardioides simplex atcc 13260, atcc 19565, and atcc 19566 are more closely related to the genus rhodococcus, especially rhodococcus erythropolis, than to the genus nocardioides. n. simplex atcc 13260 and n. simplex atcc 19565 and atcc 19566 exhibited levels of 16s rdna similarity of 99.4 and 100%, respectively, to r. erythropolis dsm 43066t. strains atcc 13260, atcc 19565, and atcc 195 ... | 1997 | 9226927 |
| comparison of tracheal aspiration with other tests for diagnosis of rhodococcus equi pneumonia in foals. | the diagnostic value of tracheal aspiration was evaluated through comparison with other diagnostic methods using an experimental model of rhodococcus equi (r. equi) pneumonia in foals. pneumonia was induced by spraying of the virulent r. equi strain atcc 33701 into the trachea of foals. all foals developed fever from 11 to 16 days after bacterial inoculation. one foal was euthanized on day 26 due to its poor prognosis, and other foals euthanized on day 43. during the experiment, some tests for d ... | 1997 | 9226846 |
| clinical manifestations, diagnosis, treatment, and prevention of rhodococcus equi infections in foals. | since the 1986 rhodococcus equi workshop, there have been major breakthroughs in understanding the epidemiology of, the virulence of, and the immune response to, this intriguing pathogen. however, with the exception of the use of hyperimmune plasma for the prevention of the disease (martens et al., 1989; madigan et al., 1991) the clinical aspects of r. equi infections have essentially remained unchanged. this article reviews the various clinical manifestations and summarizes recent advances in d ... | 1997 | 9226845 |
| pathogenicity and virulence of rhodococcus equi in foals following intratracheal challenge. | twelve foals, between 27 and 83 days old, were infected with 2 strains of rhodococcus equi by intratracheal administration. ten of the 12 foals were inoculated with 10(4)-10(10) colony forming units (cfu) of atcc 33701 strain. the other 2 foals were inoculated with 10(9) cfu of a plasmid-cured derivative of the atcc 33701 strain (atcc 33701p-). all of the 10 foals challenged with the atcc 33701 strain showed clinical signs of pulmonary disease within 5-13 days, such as gross lesions associated w ... | 1997 | 9226844 |
| macroamphiphilic cell envelope components of rhodococcus equi and closely related bacteria. | recent progress towards an understanding of the architecture of the mycobacterial cell envelope (p.j. brennan and h. nikaido, annual review of biochemistry 64 (1995) 29-63) provides a model with features more generally applicable to cell envelope organisation in other mycolic acid-containing bacteria. using this archetype, a model for the organisation of the rhodococcal cell envelope is presented here, with particular reference to cell envelope composition in rhodococcus equi. the likelihood tha ... | 1997 | 9226843 |
| in vitro production of tumor necrosis factor-alpha, interleukin-6 and interleukin-8 from normal human peripheral blood mononuclear cells stimulated by rhodococcus equi. | the capability of heat-killed rhodococcus equi organisms to induce in vitro release of tumor necrosis factor (tnf)-alpha, interleukin (il)-6 and il-8 from normal human mononuclear cells as well as the secretion kinetics of these inflammatory cytokines over a 48 h period were evaluated. results show that normal human mononuclear cells are efficiently triggered to secrete tnf-alpha, il-6 and il-8 following r. equi stimulation according to a different kinetics. in particular, release of il-b was al ... | 1997 | 9226842 |
| oxidation of macrophage membrane cholesterol by intracellular rhodococcus equi. | phagocytic uptake by cultured mouse macrophages (pd388d1) of a virulent strain (atcc 33701) of rhodococcus equi producing substantial cholesterol oxidase was accompanied by intracellular survival of the bacteria, and enzymatic oxidation of macrophage membrane cholesterol. a non-virulent strain (4219) lacking cholesterol oxidase was largely eliminated from the macrophages and did not bring about oxidation of membrane cholesterol. when r. equi 33701 was co-phagocytosed with corynebacterium pseudot ... | 1997 | 9226841 |
| pathogenesis and virulence of rhodococcus equi. | inhalation of the soil-borne organism, rhodococcus equi, can lead to a chronic and severe pyogranulomatous pneumonia in young horses and immunocompromised people. in addition, ulcerative colitis is a common sequela to infection in foals, and dissemination from the lung to other body sites is not uncommon in either the horse or man. although the facultative intracellular bacterium is susceptible to neutrophil-mediated killing, it is able to resist innate macrophage defenses and establish residenc ... | 1997 | 9226840 |
| antigenic analysis of rhodococcus equi preparations using different horse sera. | an r. equi vaccine, prepared under conditions which induce the expression of many antigens, and which has given encouraging results in field trials, was analyzed by sds-page and immunoblots and compared with other r. equi preparations: a preparation made in with the same technique from a nonvirulent isolate (virulence associated protein negative, vapa-negative); a whole cell preparation of a vapa-positive r. equi, prepared as a standard bacterin; and a semipurified vapa preparation (aptx). the a ... | 1997 | 9226839 |
| assessment of the immunogenic potential of rhodococcus equi virulence associated protein (vapa) in mice. | the development of immunity to rhodococcus equi, particularly to a virulence-associated protein (vapa) based antigen preparation, was examined in cd1 and balb/c mice after intraperitoneal vaccination. immunization with vapa based antigen without adjuvant markedly enhanced organ clearance in cd1 mice but not in balb/c mice. delayed type hypersensitivity response and antibody titres in vapa based antigen immunized balb/c mice were less than in cd1 mice. by contrast also to cd1 mice, sera from immu ... | 1997 | 9226836 |
| prevention of rhodococcus equi pneumonia of foals using two different inactivated vaccines. | two different, inactivated, aluminium salt adsorbed vaccines, one containing a r. equi strain (serotype 1, 10(9) cfu/ml and equine herpesvirus 2 (ehv-2) (1.5 x 10(7) pfu/ml) and another containing r. equi only were used on three studfarms to determine whether the disease can be prevented by vaccination of both pregnant mares and their foals. pregnant mares received two 3 ml doses of vaccine intramuscularly 6 and 2 weeks before parturition and their foals were vaccinated on two or three occasions ... | 1997 | 9226835 |
| immunoprophylaxis of rhodococcus equi pneumonia in foals. | an immunoprophylaxis program for r. equi infection of foals has been established on a number of thoroughbred breeding farms in argentina over the past 4 years. nearly 800 mares annually were immunized subcutaneously during the last 2 months of pregnancy with 2-3 doses of a vaccine containing soluble antigens of r. equi, including the virulence associated protein (vapa) and 'equi factors' exoenzymes. the mortality from r. equi pneumonia in the foals from vaccinated dams dropped from an average of ... | 1997 | 9226834 |
| protective effect against rhodococcus equi infection in mice of igg purified from horses vaccinated with virulence associated protein (vapa)-enriched antigens. | igg was purified from horses immunized with repeated doses of virulence associated (vapa) enriched antigens extracted with triton x-114 from the surface of a virulent strain of r. equi. this igg were administered to mice immunosuppressed by prior treatment with indomethacin. mice administered the higher dose were completely protected against intraperitoneal infection with r. equi; mice given the lower dose were partially protected. by contrast, mice administered concentrated nonimmune equine igg ... | 1997 | 9226833 |
| immunity to rhodococcus equi. | rhodococcal pneumonia is an important, life threatening disease of foals and immunosuppressed humans. increased knowledge of the mechanisms of protective immunity are required in order to develop an effective immunoprophylaxis strategy for horses and immunotherapeutic regiments for people. both humoral and cellular components of the immune system may be involved in immune clearance of r. equi. the susceptibility of foals less than 4-6 months of age is postulated to reflect waning maternal antibo ... | 1997 | 9226832 |
| epidemiology of rhodococcus equi infections: a review. | an overview of epidemiology of r. equi infection in foals is presented, emphasizing the importance of the virulence-associated antigens and plasmids as epidemiological markers. the monoclonal antibody-based colony blot test has been developed to identify rapidly and accurately virulent r. equi. epidemiological studies conducted during the recent 5 years have revealed that: (1) avirulent r. equi are widespread in the feces of horses and their environment on every farm; (2) the feces of horses and ... | 1997 | 9226831 |
| transposition of the is21-related element is1415 in rhodococcus erythropolis. | three copies of the is21-related transposable element is1415 were identified in rhodococcus erythropolis ni86/21. adjacent to one of the is1415 copies, a 47-bp sequence nearly identical to the conserved 5' end of integrons was found. accurate transposition of is1415 carrying a chloramphenicol resistance gene (tn5561) was demonstrated following delivery from a suicide vector to r. erythropolis sq1. | 1997 | 9226278 |
| conservation of plasmid-encoded dibenzothiophene desulfurization genes in several rhodococci. | the cloned sulfur oxidation (desulfurization) genes (sox) for dibenzothiophene (dbt) from the prototype rhodococcus sp. strain igts8 were used in southern hybridization and pcr experiments to establish the dna relatedness in six new rhodococcal isolates which are capable of utilizing dbt as a sole sulfur source for growth. the ability of these strains to desulfurize appears to be an exclusive property of a 4-kb gene locus on a large plasmid of ca. 150 kb in igts8 and ca. 100 kb in the other stra ... | 1997 | 9212438 |
| cell-linked and extracellular cholesterol oxidase activities from rhodococcus erythropolis. isolation and physiological characterization. | rhodococcus erythropolis cells growing in a cholesterol-free glycerol-containing mineral medium displayed very low levels of a cell-wall-bound cholesterol oxidase activity. addition of cholesterol induced a marked increase in the synthesis of this enzyme, which reached a maximum within 6 days and was subsequently followed by the appearance of extracellular cholesterol oxidase in the culture broth. significant levels of induction were only achieved when cholesterol emulsified with tween 80. the p ... | 1997 | 9210347 |
| rhodococcus equi and arcanobacterium haemolyticum: two "coryneform" bacteria increasingly recognized as agents of human infection. | rhodococcus equi and arcanobacterium haemolyticum, formerly classified in the genus corynebacterium, are members of the loosely defined taxon "coryneform" bacteria. although they are the etiologic agents of distinct human infections, both organisms are frequently overlooked, which results in missed or delayed diagnoses. r. equi, long known as an important pathogen of immature horses, has become in the past three decades an opportunistic pathogen of severely immunosuppressed humans. most cases ar ... | 1997 | 9204295 |
| highly efficient control of iron-containing nitrile hydratases by stoichiometric amounts of nitric oxide and light. | the reaction of two iron-containing nitrile hydratases (nhase) with no has been studied: nhase from rhodococcus sp. r312, which is probably similar to the photosensitive n771 nhase, and the new nhase from comamonas testosteroni ni1 whose aminoacid sequence is quite different from those of br312 and n771 nhases. both enzymes are equally inactivated after addition of stoichiometric amounts of no added as an anaerobic solution or produced in situ under physiological conditions by a rat brain no-syn ... | 1997 | 9202148 |
| emergence of rifampin-resistant rhodococcus equi in an infected foal. | to investigate the emergence of rifampin resistance in rhodococcus equi strains isolated from foals and their environment in japan, we compared the in vitro antimicrobial susceptibilities to rifampin of 640 isolates from 64 infected foals and 98 soil isolates from their horse-breeding farms. as a control, 39 human isolates from patients with and without aids were also tested for susceptibility to rifampin. all of the isolates showed rifampin sensitivity, except isolates from one infected foal an ... | 1997 | 9196223 |
| crystal structure of nitrile hydratase reveals a novel iron centre in a novel fold. | nitrile hydratases are unusual metalloenzymes that catalyze the hydration of nitriles to their corresponding amides. they are used as biocatalysts in acrylamide production, one of the few commercial scale bioprocesses, as well as in environmental remediation for the removal of nitriles from waste streams. nitrile hydratases are composed of two subunits, alpha and beta, and they contain one iron atom per alphabeta unit. we have determined the crystal structure of photoactivated iron-containing ni ... | 1997 | 9195885 |
| preparation of 3-ketodesogestrel metabolites by microbial transformation and chemical synthesis. | specific microbial reactions were used for the preparation of metabolites of 3-ketodesogestrel (13-ethyl-17 beta-hydroxy-11-methylene-18,19-dinor-17 alpha-pregn-4-en-20-yn-3-one, the active from of the progestagen desogestrel. clostridium paraputrificum transformed 3-ketodesogestrel (kdg) to the 5 beta-dihydro and tetrahydro metabolites 13-ethyl-17 beta-hydroxy-11-methylene-18,19-dinor-5 beta, 17 alpha-pregnan-20-yn-3-one and 13-ethyl-11-methylene-18,19-dinor-5 beta, 17 alpha-pregnan-20-yne-3 al ... | 1997 | 9178431 |
| rhodococcus equi pneumonia in a patient with occult hiv infection: successful therapy. | we report a case of rhodococcus equi cavitary pneumonia in a 37-year-old patient with occult hiv infection. because of his good immune status, the patient was given oral erythromycin and rifampin which rapidly resolved the infection. this modality of treatment may be sufficient in hiv-positive selected patients fur the resolution of rhodococcus equi pneumonia. | 1997 | 9175424 |
| optimal treatment regimen for capd peritonitis caused by rhodococcus species. | 1997 | 9175079 | |
| prevalence of the virulence-associated gene of rhodococcus equi in isolates from infected foals. | the prevalence of the plasmid-encoded virulence-associated gene (vapa) of rhodococcus equi, as determined by pcr, was found to be 98% in isolates from 154 foals with pneumonia, confirming the strong association of vapa with virulence. the vapa genes from 60 representative isolates were compared by digestion with the restriction endonuclease hinfi, and no evidence of sequence variation was detected. | 1997 | 9163507 |
| rapid identification of clinically significant species and taxa of aerobic actinomycetes, including actinomadura, gordona, nocardia, rhodococcus, streptomyces, and tsukamurella isolates, by dna amplification and restriction endonuclease analysis. | a previously described pcr-restriction fragment length polymorphism (rflp) identification schema for nocardia that used an amplified 439-bp segment (amplicon) of the 65-kda heat shock protein gene was evaluated for potential use with isolates of all clinically significant aerobic actinomycetes. the study included 28 reference (american type culture collection) strains and 198 clinical isolates belonging to 20 taxonomic groups. of these 198 isolates, 188 could be differentiated by this pcr-rflp m ... | 1997 | 9157134 |
| a stereoselective cobalt-containing nitrile hydratase. | nitrile hydratase from pseudomonas putida nrrl-18668 has been purified and characterized. the purified enzyme catalyzes the hydration of 2(s)-(4'-chlorophenyl)-3-methylbutyronitrile at least fifty times faster than that of 2(r)-(4'-chlorophenyl)-3-methylbutyronitrile. this enzyme is a member of the class of nitrile hydratase that contains cobalt. visible absorption and cd spectra suggest the cobalt exists as a non-corrin low-spin co3+ ion in a tetragonally-distorted octahedral ligand field. chem ... | 1997 | 9154927 |
| [review of 105 cases of isolation of rhodococcus equi in humans]. | rhodococcus equi (formerly corynebacterium) has been long considered an exclusively zoopathogenic microbe causing mainly granulomatous pneumonias and lung abscesses in young foals. the aim of this paper was to analyse substantial features of r, equi infections hitherto reported in man. | 1997 | 9147854 |
| virulent and avirulent rhodococcus equi infection in t-cell deficient athymic nude mice: pathologic, bacteriologic and immunologic responses. | we investigated the pathologic, bacteriologic and immunologic responses of balb/c-nu/nu mice (nude mice) and balb/c mice (euthymic mice) infected intravenously with virulent and avirulent rhodococcus equi atcc 33701, and its plasmid-cured derivative atcc 33701p-, to evaluate the role of t lymphocytes. adaptive transfer of immune and normal spleen cells into nude mice was also investigated. nude and euthymic mice were inoculated with 10(6) atcc 33701 or 10(6) atcc 33701p- intravenously (i.v.) and ... | 1997 | 9143883 |
| molecular analysis of the rhodococcus sp. strain h1 her gene and characterization of its product, a heroin esterase, expressed in escherichia coli. | the structural gene for heroin esterase was cloned from rhodococcus sp. strain h1 and expressed in escherichia coli bl21(de3). the purified enzyme was found to be a tetramer with an m(r) of 137,000 and an apparent k(m) of 0.88 mm for 6-acetylmorphine. the g-x-s-x-g motif was observed in the deduced amino acid sequence, suggesting that the enzyme is a serin esterase. | 1997 | 9143135 |
| thiocarbamate herbicide-inducible nonheme haloperoxidase of rhodococcus erythropolis ni86/21. | during biodegradation of thiocarbamate herbicides by rhodococcus erythropolis ni86/21, a protein with an m(r) of 30,000 is induced (i. nagy, g. schoofs, f. compernolle, p. proost, j. vanderleyden, and r.de mot, j. bacteriol. 177:676-687, 1995). based on n-terminal sequence data for the protein purified by two-dimensional electrophoresis, the corresponding structural gene, thcf, was cloned and sequenced. the deduced protein sequence of thcf is homologous to those of nonheme haloperoxidases. a par ... | 1997 | 9143122 |
| genes encoding the nad-reducing hydrogenase of rhodococcus opacus mr11. | the dissociation of the soluble nad-reducing hydrogenase of rhodococcus opacus mr11 into two dimeric proteins with different catalytic activities and cofactor composition is unique among the nad-reducing hydrogenases studied so far. the genes of the soluble hydrogenase were localized on a 7.4 kbp asnl fragment of the linear plasmid phg201 via heterologous hybridization. analysis of the nucleotide sequence of this fragment revealed the seven open reading frames orf1, hoxf, -u, -y, -h, -w and orf7 ... | 1997 | 9141690 |
| [characterization of rhodococcus equi isolates from horse and man]. | in the present investigation 19 and 22 r. equi-cultures isolated from diseased horses and humans, respectively, could be correctly identified by their morphological, biochemical and serological properties. the rod-coccus life cycle appeared to be a common feature of almost all cultures investigated. the cultures were typeable with the typing system described by prescott (1981). the predominant serotypes among the r. equi-isolates belonged to serotypes 1 and 2. among the r. equi-isolates from hor ... | 1997 | 9139629 |
| location, catalytic activity, and subunit composition of nad-reducing hydrogenases of some alcaligenes strains and rhodococcus opacus mr22. | six new strains of alcaligenes enriched for and isolated as nickel-resistant bacteria resemble alcaligenes eutrophus h16 and contain both an nad-reducing, tetrameric soluble hydrogenase and a membrane-bound hydrogenase. none of the soluble hydrogenases share with the rhodococcus opacus mr11 enzyme tetramer the property of being cleaved easily into two dimeric moieties [a hydrogenase (betadelta) and an nadh:acceptor oxidoreductase (alphagamma)], in the absence of nickel or at low ionic strength. ... | 1997 | 9133325 |
| acute mediastinitis due to rhodococcus equi in a patient with human immunodeficiency virus infection. | a patient with human immunodeficiency virus infection developed anterior mediastinitis during antibiotic treatment for empyema due to rhodococcus equi. this is the first reported case of infection with this organism in this setting. despite an adequate course of therapy and maintenance treatment with antibiotics to which the isolate of rhodococcus equi was susceptible in vitro, the patient experienced relapse of the infection into the mediastinum. clinicians should consider this complication whe ... | 1997 | 9131329 |
| pathogenetic role of phagocytic abnormalities in human virus immunodeficiency infection: possible therapeutical approaches. a review. | polymorphonuclear cells (pmn) and monocytes/macrophages (m/m) represent the first defence line against invading microorganisms. both phagocytic cell functions are precociously compromised in human immunodeficiency virus (hiv)-infected subjects, thus leading to infectious and neurological complications in the late stages of disease. among intracellular pathogens, emerging bacteria such as bartonella henselae and rhodococcus equi can cause peculiar clinical pictures, i.e. the bacillary parenchimal ... | 1997 | 9130002 |
| strategy for detection and identification of bacteria based on 16s rrna genes in suspected cases of whipple's disease. | the 16s ribosomal rna (rrna) gene of the phylogenetic subdivision containing gram-positive bacteria with a high g + c content was detected specifically in clinical specimens from patients suspected of having whipple's disease. the primary structure of 16s rdna amplified from clinical samples was determined by cloning and sequencing. two sorts of sequences were identified: one corresponded exactly to the rrna sequence of tropheryma whippelii (genbank accession no. m87484) while the other was rela ... | 1997 | 9128199 |
| rhodococcus equi infections: antibiotic therapy and relapses. | 1997 | 9110086 | |
| pulmonary malacoplakia associated with rhodococcus equi infection in an aids patient. report of a case with diagnosis by fine needle aspiration. | malacoplakia is an uncommon, peculiar inflammatory disease with few cytologic descriptions. it is seen mostly in the lower urinary tract, with rare occurrences in the lungs. escherichia coli is the most common underlying etiologic agent, with only a few cases reported with rhodococcus equi infection. | 1997 | 9100789 |
| use of rhodococcus equi virulence-associated protein for immunization of foals against r equi pneumonia. | to evaluate use of the virulence-associated protein of rhodococcus equi in immunizing foals against r equi pneumonia. | 1997 | 9099378 |
| characterization of the genes encoding a receptor-like histidine kinase and a cognate response regulator from a biphenyl/polychlorobiphenyl-degrading bacterium, rhodococcus sp. strain m5. | we report the cloning, sequence, and expression of the bpds and bpdt genes from rhodococcus sp. strain m5, which are believed to encode the first two-component signal transduction system in the genus rhodococcus, which potentially regulates biphenyl/polychlorobiphenyl metabolism in m5. bpdt has a typical responses regulator sequence (209 amino acids; 23 kda), whereas bpds, the predicted histidine kinase component, is an unusually large transmembrane protein (1,576 amino acids; 170 kda) that cont ... | 1997 | 9098081 |
| rhodococcus equi pneumonia. | corynebacterium equi is a pleomorphic gram-positive rod that was first isolated in 1923 by magnusson, and is the cause of suppurative broncho-pneumonia in foals. the organism, now know as rhodococcus equi, is ubiquitous in nature and is increasingly recognized as pathogenic, particularly in the immunocompromised population. cavitary pneumonia clinically resembling mycobacterium tuberculosis is the most common manifestation of r equi disease, but extrapulmonary infections are seen as well. long t ... | 1997 | 9097379 |
| prevention of insect-borne disease: an approach using transgenic symbiotic bacteria. | expression of molecules with antiparasitic activity by genetically transformed symbiotic bacteria of disease-transmitting insects may serve as a powerful approach to control certain arthropod-borne diseases. the endosymbiont of the chagas disease vector, rhodnius prolixus, has been transformed to express cecropin a, a peptide lethal to the parasite, trypanosoma cruzi. in insects carrying the transformed bacteria, cecropin a expression results in elimination or reduction in number of t. cruzi. a ... | 1997 | 9096383 |
| bacterial infections. | non-opportunistic bacterial infections are an important cause of morbidity and mortality for hiv-infected adults and children. factors associated with increased risk of these include altered b- and t-cell function; altered phagocytic cell function; skin and mucous membrane defects; and use of indwelling vascular catheters, antibiotics, or cytotoxic agents. the pathogens encountered most frequently are s. aureus, s. pneumoniae, h. influenzae, salmonella sp., and pseudomonas aeruginosa. less commo ... | 1997 | 9093231 |
| long survival of a patient with aids in spite of previous rhodococcus equi infection. | 1997 | 9085620 | |
| acetylene degradation by new isolates of aerobic bacteria and comparison of acetylene hydratase enzymes. | aerobic acetylene-degrading bacteria were isolated from soil samples. two isolates were assigned to the species rhodococcus opacus, two others to rhodococcus ruber and gordona sp. they were compared with known strains of aerobic acetylene-, cyanide-, or nitrile-utilizing bacteria. the acetylene hydratases of r opacus could be measured in cell-free extracts only in the presence of a strong reductant like titanium(iii) citrate. expression of these enzymes was molybdenum-dependent. acetylene hydrat ... | 1997 | 9084145 |
| differentiation of human promyelocytic leukemia cell line hl60 by microbial extracellular glycolipids. | microbial extracellular glycolipids, succinoyl trehalose lipid (stl), and mannosylerythritol lipid (mel) inhibited the growth of a human promyelocytic leukemia cell line, hl60, and induced their morphological changes. the results of specific and nonspecific leukocyte esterase activities showed that stl induced monocytotic differentiation while mel induced granulocytic differentiation. stl and mel markedly increased common differentiation-associated characteristics in monocytes and granulocytes, ... | 1997 | 9076663 |
| the bphdef meta-cleavage pathway genes involved in biphenyl/polychlorinated biphenyl degradation are located on a linear plasmid and separated from the initial bphacb genes in rhodococcus sp. strain rha1. | the bphacb genes responsible for the initial oxidation of the aromatic ring of biphenyl/polychlorinated biphenyls (pcb) to meta-cleavage product in rhodococcus sp. rha1 have been characterized. we cloned the 6.1 kb ecori fragment containing another extradiol dioxygenase gene (etbc) which was induced during the growth on ethylbenzene. the bphd, bphe and bphf encoding 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate (hopd) hydrolase, 2-hydroxypenta-2,4-dienoate hydratase and 4-hydroxy-2-oxovalerate aldol ... | 1997 | 9073078 |
| [structure and antigenicity of the major glycolipid from opportunistic bacteria rhodococcus equi]. | some results from structural and immunological studies of the major glycolipid from rhodococcus equi were presented. this glycolipid showed to be a glucosylmonomycolate (gmm) with an aliphatic chain c38. its value as taxonomic and immunodiagnostic marker was discussed. | 1996 | 9072772 |
| heterologous expression of biphenyl dioxygenase-encoding genes from a gram-positive broad-spectrum polychlorinated biphenyl degrader and characterization of chlorobiphenyl oxidation by the gene products. | the bpha1a2a3a4 gene cluster, encoding a biphenyl dioxygenase from rhodococcus globerulus p6, a gram-positive microorganism able to degrade a wide spectrum of polychlorobiphenyls (pcbs), was expressed in pseudomonas putida, thereby allowing characterization of chlorobiphenyl oxidation by this enzyme. while p6 biphenyl dioxygenase activity was observed in p. putida containing bpha1a2a3a4, no activity was detected in escherichia coli cells containing the same gene cluster. in e. coli, transcriptio ... | 1997 | 9068637 |
| bacterial degradation of emulsified crude oil and the effect of various surfactants. | a rhodococcus sp. 094 bacterium was tested for its ability to oxidize alkanes in crude oil emulsified by nonionic chemical and biological surfactants. oxidation rates were measured in a 3-h period by warburg respirometry. 14co2 recovery was measured from the [1-14c]hexadecane spiked crude oil. response to emulsified oil depended on the physiological state of the bacteria (i.e., cells harvested in the exponential and stationary growth phases) were tested. oxidation rates by cells in the exponenti ... | 1997 | 9057292 |
| radiologic features of rhodococcus equi pneumonia in aids. | this report outlines the radiological features observed in three cases of rhodococcus equi (r. equi) pneumonia in aids (acquired immunodeficiency syndrome) and reviews another 45 radiological reports published of this emerging opportunistic pneumonia in human immunodeficiency virus (hiv) infected patients. the clinical signs in our three patients consisted in a subacute onset of respiratory symptoms and fever. a low lymphocyte count (< 200 cells/mm3), pulmonary infiltrates, and pleural effusion ... | 1997 | 9056153 |
| equine herpesvirus type 2: prevalence and seroepidemiology in foals. | whole blood and serum were collected from foals to determine the prevalence of equine herpesvirus type 2 (ehv 2) infection in foals, age at which infection can first be identified and serological responses to infection. equine herpesvirus type 2 was isolated from peripheral blood mononuclear cells (pbmc) from 68 of 69 foals, 1-8-months-old, sampled once. virus isolation was performed twice at intervals of 2-7 months on pbmcs from 33 foals and ehv2 was isolated on both occasions in all but one fo ... | 1996 | 9049491 |
| location, catalytic activity, and subunit composition of nad-reducing hydrogenases of some alcaligenes strains and rhodococcus opacus mr22 | six new strains of alcaligenes enriched for and isolated as nickel-resistant bacteria resemble alcaligenes eutrophus h16 and contain both an nad-reducing, tetrameric soluble hydrogenase and a membrane-bound hydrogenase. none of the soluble hydrogenases share with the rhodococcus opacus mr11 enzyme tetramer the property of being cleaved easily into two dimeric moieties [a hydrogenase (betadelta) and an nadh:acceptor oxidoreductase (alphagamma)], in the absence of nickel or at low ionic strength. ... | 1997 | 9042758 |
| restriction enzyme analysis of the virulence plasmids of vapa-positive rhodococcus equi strains isolated from humans and horses. | restriction enzyme digestion patterns of the large virulence plasmids of 8 human and 37 foal isolates of virulence-associated protein (vapa)-positive rhodococcus equi strains from different sources were compared. foal isolates came from five continents. digestion with ecori divided these plasmids into three closely related types, and digestion with bamhi divided them into three major types which corresponded to the ecori types. the only ecori and bamhi type 3 plasmid was from a single foal isola ... | 1997 | 9041424 |
| identification of yeasts and coryneform bacteria from the surface microflora of brick cheeses. | coryneform bacteria and yeasts of 21 brick cheeses from six german dairies, produced by using undefined ripening cultures, were identified. arthrobacter nicotianae, brevibacterium linens, corynebacterium ammoniagenes, corynebacterium variabilis and rhodococcus fascians were found in significant numbers. out of 148 coryneform isolates 36 could not be identified at the species level. with the exception of a large rennet cheese, the coryneform microflora of rennet and acid cured cheeses were simila ... | 1997 | 9039559 |
| biodegradation of methyl t-butyl ether by pure bacterial cultures. | three pure bacterial cultures degrading methyl t-butyl ether (mtbe) were isolated from activated sludge and fruit of the gingko tree. they have been classified as belonging to the genuses methylobacterium, rhodococcus, and arthrobacter. these cultures degraded 60 ppm mtbe in 1-2 weeks of incubation at 23-25 degrees c. the growth of the isolates on mtbe as sole carbon source is very slow compared with growth on nutrient-rich medium. uniformly-labeled [14c]mtbe was used to determine 14co2 evolutio ... | 1997 | 9035411 |
| classification of catechol 1,2-dioxygenase family: sequence analysis of a gene for the catechol 1,2-dioxygenase showing high specificity for methylcatechols from gram+ aniline-assimilating rhodococcus erythropolis an-13. | gram+ aniline-assimilating rhodococcus erythropolis an-13 (an-13) produces catechol 1,2-dioxygenase (c12o) showing high enzymatic activities for 3- and 4-methylcatechols [aoki et al. (1984) agric. biol. chem. 48, 2087-2095]. a 3.0 kb sau3ai fragment carrying a gene encoding c12o(cata) was cloned by selection of transformants showing c12o activity from a gene library of an-13. furthermore, we specified a 1.6 kb sali fragment containing cata from the sau3ai fragment by subcloning. sequence analysi ... | 1997 | 9034312 |
| chronic pneumonia caused by rhodococcus equi in a patient without impaired immunity. | a 48 year old, human immunodeficiency virus (hiv)-negative, immunocompetent male patient had a chronic progressive pulmonary infiltrate, without radiological cavitation, in the middle lobe of the right lung produced by rhodococcus equi. he reported direct contact with a diseased dog. the patient was diagnosed by thoracotomy and treated by lobectomy. after 16 months of follow-up, the patient was asymptomatic and had neither recurrence nor immunological disturbances. | 1997 | 9032523 |
| rhodococcus or mycobacterium? an example of misdiagnosis in hiv infection. | 1997 | 9030376 | |
| [photoreactive nitrite hydratase]. | 1997 | 9028170 | |
| the plasmid-located haloalkane dehalogenase gene from rhodococcus rhodochrous ncimb 13064. | the haloalkane dehalogenase (dhaa) gene from rhodococcus rhodochrous ncimb 13064 was cloned and sequenced. its comparison with the previously studied dhla gene from xanthobacter autotrophicus gj10 did not show homology. however, the amino acid sequences of the products of these genes showed approximately 30% identity and several of the catalytic amino acid residues were conserved in the ncimb 13,064 dehalogenase. a high level of dhaa expression was demonstrated in escherichia coli cells and this ... | 1997 | 9025284 |
| a flavin reductase stimulates dsza and dszc proteins of rhodococcus erythropolis igts8 in vitro. | rhodococcus erythropolis igts8 is a gram positive bacterium, which can catabolize dibenzothiophene to 2-hydroxybiphenyl and inorganic sulfur without the cleavage of carbon-carbon bonds. three structural genes, dsza, dszb, and dszc, have been cloned and shown to be necessary for this phenotype. here, we demonstrate that a fmn:nadph oxidoreductase from vibrio harveyi complements activities of purified dsza and dszc proteins. furthermore, we propose that dsza and dszc are oxygenase units that do no ... | 1997 | 9020064 |
| interpretation of chest radiographs in aids patients: usefulness of cd4 lymphocyte counts. | specific infections and neoplasms that are complications of acquired immunodeficiency syndrome (aids) occur within various cd4 lymphocyte count ranges. knowledge of how these counts correlate with radiographic appearances of these entities can limit the differential diagnosis because certain conditions are uncommon above a specific count. in patients with cd4 lymphocyte counts above 200 cells/mm3 and radiographic findings of cavitary and noncavitary consolidation, bacterial pneumonia and mycobac ... | 1997 | 9017798 |
| biodegradation of polyhydroxyalkanoic acids. | stimulated by the commercial availability of bacteriologically produced polyesters such as poly[(r)-3-hydroxybutyric acid], and encouraged by the discovery of new constituents of polyhydroxyalkanoic acids (pha), a considerable body of knowledge on the metabolism of pha in microorganisms has accumulated. the objective of this essay is to give an overview on the biodegradation of pha. the following topics are discussed: (i) general considerations of pha degradation, (ii) methods for identification ... | 1996 | 9008883 |