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instantlabs listeria species food safety kit. performance tested methods 041304.the instantlabs listeria species food safetykitwas validated against the international organization for standardization (iso) reference method 11290-1 for the detection of listeria monocytogenes and other listeria species. the matrixes (stainless steel, sealed concrete, cheddar cheese, raw shrimp, and hot dogs) were inoculated with approximately 1 cfu/test portion of various listeria species to generate fractional positives (5-15) in 20 inoculated samples. enrichments were also fractionally inoc ...201425051633
deletion of the membrane protein lmo0412 increases the virulence of listeria monocytogenes.listeria monocytogenes is a facultative intracellular pathogen that causes gastroenteritis, meningitis, encephalitis and maternofetal infections. 20-30% of eubacterial orfs are predicted to encode membrane proteins. the bacterial cytoplasmic membrane is a macromolecular structure, which plays a key role for the pathogenesis. despite this, little knowledge exists regarding the function of cytoplasmic membrane proteins of listeria during infection. here, we investigated a predicted membrane protei ...201425051038
assessment of oligogalacturonide from citrus pectin as a potential antibacterial agent against foodborne pathogens.foodborne diseases are an important public health problem in the world. the bacterial resistance against presently used antibiotics is becoming a public health issue; hence, the discovery of new antimicrobial agents from natural sources attracts a lot of attention. antibacterial activities of oligogalacturonide from commercial microbial pectic enzyme (cpe) treated citrus pectin, which exhibits antioxidant and antitumor activities, against 4 foodborne pathogens including salmonella typhimurium, s ...201425048440
serogrouping and pulsed-field gel electrophoresis analysis of listeria monocytogenes isolates from cases of human infection in hungary 2004–2012 molecular typing of listeria monocytogenes in hungary.eighty isolates of listeria monocytogenes cultured from human infections in hungary between 2004 and 2012 were serotyped by the pcr technique of doumithet al. [9] and characterised by pulsed-field gel electrophoresis (pfge). most of the isolates belonged to two serogroups: 53 isolates (66.3%) to serovar group 4b,4d,4e and 21isolates (25.8%) to serogroup 1/2a,3a. although many pulsotypes were identified a particular pulsotype proved highly excelling comprising of 31 isolates after digestion by bo ...201425046881
dnase i and proteinase k impair listeria monocytogenes biofilm formation and induce dispersal of pre-existing biofilms.current sanitation methods in the food industry are not always sufficient for prevention or dispersal of listeria monocytogenes biofilms. here, we determined if prevention of adherence or dispersal of existing biofilms could occur if biofilm matrix components were disrupted enzymatically. addition of dnase during biofilm formation reduced attachment (<50% of control) to polystyrene. treatment of established 72h biofilms with 100μg/ml of dnase for 24h induced incomplete biofilm dispersal, with <2 ...201425043896
use of mild irradiation doses to control pathogenic bacteria on meat trimmings for production of patties aiming at provoking minimal changes in quality attributes.the objectives of the present work were to assess the use of moderate doses of gamma irradiation (2 to 5 kgy) and to reduce the risk of pathogen presence without altering the quality attributes of bovine trimmings and of patties made of irradiated trimmings. microbiological indicators (coliforms, pseudomonas spp and mesophilic aerobic counts), physicochemical indicators (ph, color and tiobarbituric acid) and sensory changes were evaluated during storage. 5 kgy irradiation doses slightly increase ...201425042241
rapid detection of listeria monocytogenes in raw milk and soft cheese by a redox potential measurement based method combined with real-time pcr.the incidence of outbreaks of foodborne listeriosis has indicated the need for a reliable and rapid detection of the microbe in different foodstuffs. a method combining redox potential measurement and real-time polymerase chain reaction (pcr) was developed to detect listeria monocytogenes in artificially contaminated raw milk and soft cheese. food samples of 25 g or 25 ml were homogenised in 225 ml of listeria enrichment broth (leb) with oxford supplement, and the redox potential measurement tec ...201425038948
delivery of phytochemicals of tropical fruit by-products using poly (dl-lactide-co-glycolide) (plga) nanoparticles: synthesis, characterization, and antimicrobial activity.nanoencapsulation offers great potential in natural compounds delivery as it protects them from degradation, improves their aqueous solubility, and delivers active compounds to the action site. poly (dl-lactide-co-glycolide) (plga) nanoparticles of acerola, guava, and passion fruit by-product extracts were synthesized using the emulsion-evaporation method. plga with different lactide to glycolide (50:50 and 65:35) ratios were used to determine how polymer composition affected nanoparticles prope ...201425038688
antioxidant and antibacterial activities of sulphated polysaccharides from pleurotus eryngii and streptococcus thermophilus ascc 1275.polysaccharides from pleurotus eryngii (peps) and exopolysaccharides from streptococcus thermophilus ascc 1275 (st1275 eps) were sulphated, and antioxidant and antibacterial activities of sulphated and crude polysaccharides were determined. degree of sulphonation of peps and st1275 eps was 0.69 and 0.31, respectively. characteristic bands in ft-ir spectra indicated that the sulphate group was at the c6 position of the galactose skeleton. antioxidant activities of peps and st1275 eps were signifi ...201425038675
inhibitory effects of the κ-casein macropeptide isolated from milk protein on the biofilm formation and virulence of listeria monocytogenes.we demonstrate the inhibitory effects of κ-casein macropeptide (cmp) on the biofilm formation and virulence of listeria monocytogenes scott a. the inhibition of biofilm formation by cmp was initially investigated by using the protocol applied for the 96-well microtiter plate assay. low concentrations of cmp (0.1, 0.2, 0.3, 0.4, and 0.5 mg/ml) that were tested resulted in a profound inhibitory effect on biofilm formation at a concentration of 0.4 mg/ml. cmp also significantly repressed the transc ...201425036840
serial transfer of single-cell-derived immunocompetence reveals stemness of cd8(+) central memory t cells.maintenance of immunological memory has been proposed to rely on stem-cell-like lymphocytes. however, data supporting this hypothesis are focused on the developmental potential of lymphocyte populations and are thus insufficient to establish the functional hallmarks of stemness. here, we investigated self-renewal capacity and multipotency of individual memory lymphocytes by in vivo fate mapping of cd8(+) t cells and their descendants across three generations of serial single-cell adoptive transf ...201425035956
a multicopy srna of listeria monocytogenes regulates expression of the virulence adhesin lapb.the multicopy srna lhrc of the intracellular pathogen listeria monocytogenes has been shown to be induced under infection-relevant conditions, but its physiological role and mechanism of action is not understood. in an attempt to pinpoint the exact terms of lhrc expression, cell envelope stress could be defined as a specific inducer of lhrc. in this process, the two-component system lisrk was shown to be indispensable for expression of all five copies of lhrc. lapb mrna, encoding a cell wall ass ...201425034691
the effect of oleanolic and ursolic acids on the hemolytic properties and biofilm formation of listeria monocytogenes.oleanolic acid and ursolic acid are pentacyclic triterpenoids isolated from a variety of medicinal plants, which have antibacterial activity. listeria monocytogenes is a gram-positive facultative pathogen, being the causative agent of listeriosis. the present study was carried out to evaluate the in vitro effect of sub-inhibitory concentrations of both triterpene acids on the pathogenicity determinants of l. monocytogenes: their hemolytic activity and biofilm forming ability. oleanolic and ursol ...201425033658
in-vitro assessment of antioxidant and antimicrobial activities of methanol extracts and essential oil of thymus hirtus sp. algeriensis.owing to the complexity of the antioxidant materials and their mechanism of actions, it is obvious that no single testing method is capable of providing a comprehensive picture of the antioxidant profile. the essential oil of the thymus specie may still possess other important activities in traditional medicine, it can be used in the treatment of fever and cough. this essential oil may also have an anticancer activity.201425022197
bacterial foodborne infections after hematopoietic cell transplantation.diarrhea, abdominal pain, and fever are common among patients undergoing hematopoietic cell transplantation (hct), but such symptoms are also typical with foodborne infections. the burden of disease caused by foodborne infections in patients undergoing hct is unknown. we sought to describe bacterial foodborne infection incidence after transplantation within a single-center population of hct recipients. all hct recipients who underwent transplantation from 2001 through 2011 at the fred hutchinson ...201425020101
bi-phasic growth of listeria monocytogenes in chemically defined medium at low temperatures.the present work reports a novel observation regarding the growth of l. monocytogenes in modified welshimer's broth (mwb) at low temperatures. specifically, the direct monitoring of the growth of l. monocytogenes scott a using plate count data revealed that the pathogen displays a bi-phasic growth pattern in mwb at 7 °c. this bi-phasic growth pattern is masked (not observed) when optical density (od) measurements are used to monitor growth due to the inability of od readings to detect l. monocyt ...201425016210
analysing and modelling the growth behaviour of listeria monocytogenes on rte cooked meat products after a high pressure treatment at 400 mpa.various predictive models are available for high pressure inactivation of listeria monocytogenes in food, but currently available models do not consider the growth kinetics of surviving cells during the subsequent storage of products. therefore, we characterised the growth of l. monocytogenes in sliced cooked meat products after a pressurization treatment. two inoculum levels (10(7) or 10(4) cfu/g) and two physiological states before pressurization (freeze-stressed or cold-adapted) were evaluate ...201425016207
assessment of salmonella and listeria monocytogenes level in ready-to-cook poultry meat: effect of various high pressure treatments and potassium lactate concentrations.the objective of this study was to develop a probabilistic model in order to determine the contamination level of salmonella and listeria monocytogenes in ready-to-cook poultry meat, after a high pressure (hp) treatment. the model included four steps: i) reception of raw meat materials, mincing and mixing meat, ii) partitioning and packaging into 200-g modified atmosphere packs, iii) high pressure treatment of the meat, and iv) storage in chilled conditions until the end of the shelf-life. the m ...201425016206
[seroprevalance of brucellosis, listeriosis and toxoplasmosis in cattle in adana province of turkey].this study was conducted to identify the seroprevalance of diseases which are zoonotic and responsible from abortion such as toxoplasmosis, listeriosis and brucellosis in holstein crossbred cattle of different age and sex in adana province, between 2008 april-september.201425016114
purification and mass spectrometry based characterization of a pediocin produced by pediococcus acidilactici 13.bacteriocins are antimicrobial peptides produced by several bacterial species. among the bacteriocins pediocin-like bacteriocins have a significant inhibitory activity on the foodborne pathogens especially on listeria monocytogenes. this study aims to select a simple and usable purification method to purify/concentrate the antimicrobial peptide and characterization of the bacteriocin produced by pediococcus acidilactici 13 by using proteomic approaches which is a recent omic technology. for puri ...201425015266
a btla-mediated bait and switch strategy permits listeria expansion in cd8α(+) dcs to promote long-term t cell responses.listeria monocytogenes infected cd8α(+) dcs in the spleen are essential for cd8(+) t cell generation. cd8α(+) dcs are also necessary for listeria expansion and dissemination within the host. the mechanisms that regulate cd8α(+) dcs to allow listeria expansion are unclear. we find that activating the b and t lymphocyte attenuator (btla), a coinhibitory receptor for t cells, suppresses, while blocking btla enhances, both the primary and memory cd8 t cell responses against listeria. btla(-/-) mice ...201425011109
characterization of listeria monocytogenes strains isolated during 2011-2013 from human infections in switzerland.listeria monocytogenes, an emerging foodborne pathogen, can cause in the population at risk severe infections that are associated with high case fatality rates. a total of 93 l. monocytogenes strains isolated from different patients in switzerland from july 2011 to september 2013 were further characterized. septicemia was reported for 74.2% of the patients, meningitis for 10.8%, and abortion for 3.2%. the majority of the strains belonged to serotype 1/2a (n=58) followed by serotype 4b (n=28), 1/ ...201425007293
reservoirs of listeria species in three environmental ecosystems.soil and water are suggested to represent pivotal niches for the transmission of listeria monocytogenes to plant material, animals, and the food chain. in the present study, 467 soil and 68 water samples were collected in 12 distinct geological and ecological sites in austria from 2007 to 2009. listeria was present in 30% and 26% of the investigated soil and water samples, respectively. generally, the most dominant species in soil and water samples were listeria seeligeri, l. innocua, and l. iva ...201425002422
the potential probiotic lactobacillus rhamnosus ctc1679 survives the passage through the gastrointestinal tract and its use as starter culture results in safe nutritionally enhanced fermented sausages.the human-derived potential probiotic strain lactobacillus rhamnosus ctc1679 was used as a starter culture in reduced fat and sodium low-acid fermented sausages (fuets) to assess its ability to survive through the gastrointestinal tract (git) in a human intervention study consisting of 5 healthy volunteers who consumed 25 g fuet a day for 21 days. faecal samples were analysed during and after consumption. l. rhamnosus ctc1679 produced a transient colonisation of the human git and persisted durin ...201424998181
functional properties and stability of spray-dried pigments from bordo grape (vitis labrusca) winemaking pomace.the stability of anthocyanin and phenolic compounds, the antioxidant capacity, the antimicrobial activity and the capacity to inhibit arginase from leishmania were evaluated in spray-dried powders from bordo grape winemaking pomace extract. the pigments were produced using maltodextrin as the carrier agent at concentrations varying from 10% to 30% and air entrance temperatures varying from 130 to 170°c. a sample of freeze-dried extract without the carrier was also evaluated. the anthocyanins in ...201424996348
response to comment on "tim-3 directly enhances cd8 t cell responses to acute listeria monocytogenes infection". 201424994908
comment on "tim-3 directly enhances cd8 t cell responses to acute listeria monocytogenes infection". 201424994907
perinatal immunization with vaccine-grade listeria monocytogenes provides protection against murine th2 airway inflammation.asthma is a chronic respiratory disorder that leads to inflammation and narrowing of the airways. its global prevalence has attained epidemic levels and treatment options that reach beyond temporary relief of symptoms are urgently needed. since the processes leading to clinically symptomatic asthma start early in life, we set out to systematically evaluate a neonatal immunotherapeutic based on listeria monocytogenes (lm) for the control of allergic sensitization.201424991458
schistosoma mansoni soluble egg antigens enhance listeria monocytogenes vector hiv-1 vaccine induction of cytotoxic t cells.vaccines are an important public health measure for prevention and treatment of diseases. in addition to the vaccine immunogen, many vaccines incorporate adjuvants to stimulate the recipient's immune system and enhance vaccine-specific responses. while vaccine development has advanced from attenuated organism to recombinant protein or use of plasmid dna, the development of new adjuvants that safely increase immune responses has not kept pace. previous studies have shown that the complex mixture ...201424990901
biosynthesis of silver nanoparticles using lingonberry and cranberry juices and their antimicrobial activity.in this study lingonberry and cranberry juices were used for silver nanoparticle synthesis. the berry juices were characterized by total phenolics, total anthocyanins and benzoic acid content, respectively 1.9-2.7mg/ml, 55.2-83.4mg/l and 590.8-889.2mg/l. the synthesis of silver nanoparticles was performed at room temperature assisting in solutions irradiated by ultraviolet for 30min. ultraviolet-visible (uv-vis) spectroscopy and microscopy confirmed the formation of nanoparticles as well as the ...201424988412
inactivation kinetics of escherichia coli o157:h7, salmonella enterica serovar typhimurium, and listeria monocytogenes in ready-to-eat sliced ham by near-infrared heating at different radiation intensities.the aim of this study was to investigate the inactivation kinetics of salmonella enterica serovar typhimurium, escherichia coli o157:h7, and listeria monocytogenes on ready-to-eat sliced ham by near-infrared (nir) heating as a function of the processing parameter, radiation intensity. precooked ham slices inoculated with the three pathogens were treated at different nir intensities (ca. 100, 150, and 200 μw/cm(2)/nm). an increase in the applied radiation intensity resulted in a gradual increase ...201424988034
metal-chelating active packaging film enhances lysozyme inhibition of listeria monocytogenes.several studies have demonstrated that metal chelators enhance the antimicrobial activity of lysozyme. this study examined the effect of metal-chelating active packaging film on the antimicrobial activity of lysozyme against listeria monocytogenes. polypropylene films were surface modified by photoinitiated graft polymerization of acrylic acid (pp-g-paa) from the food contact surface of the films to impart chelating activity based on electrostatic interactions. pp-g-paa exhibited a carboxylic ac ...201424988022
low occurrence of listeria monocytogenes on bovine hides and carcasses in minas gerais state, brazil: molecular characterization and antimicrobial resistance.listeria monocytogenes occurrence was assessed in three slaughterhouses located in minas gerais state, brazil, by analysis of 209 bovine carcasses. four sponge samples were obtained from each carcass in different steps (a, from hide, before bleeding; b, after hide removal; c, after evisceration; and d, after end washing), resulting in a total of 836 samples. the samples were tested for the presence of l. monocytogenes according to the international organization for standardization 11290-1, and p ...201424988021
phenotypic and genotypic characterization of atypical listeria monocytogenes and listeria innocua isolated from swine slaughterhouses and meat markets.in the last decade, atypical listeria monocytogenes and l. innocua strains have been detected in food and the environment. because of mutations in the major virulence genes, these strains have different virulence intensities in eukaryotic cells. in this study, we performed phenotypic and genotypic characterization of atypical l. monocytogenes and l. innocua isolates obtained from swine slaughterhouses and meat markets. forty strains were studied, including isolates of l. monocytogenes and l. inn ...201424987702
the receptor for the complement c3a anaphylatoxin (c3ar) provides host protection against listeria monocytogenes-induced apoptosis.listeria monocytogenes is a gram-positive intracellular bacterium that is acquired through tainted food and may lead to systemic infection and possible death. despite the importance of the innate immune system in fighting l. monocytogenes infection, little is known about the role of complement and its activation products, including the potent c3a anaphylatoxin. in a model of systemic l. monocytogenes infection, we show that mice lacking the receptor for c3a (c3ar(-/-)) are significantly more sen ...201424981453
purification of a novel chitin-binding lectin with antimicrobial and antibiofilm activities from a bangladeshi cultivar of potato (solanum tuberosum).a new chitin-binding lectin was purified from a bangladeshi cultivar 'deshi' of potato (solanum tuberosum l.) through anion-exchange and affinity chromatographies using a chitin column. sodium dodecyl sulphate-polyacrylamide gel electrophoresis (sds-page) showed the molecular mass of the lectin as 20,000 daltons. this molecular mass was almost half of the molecular masses of chitin-binding lectins derived from other potatoes. the lectin showed both bactericidal and growth-inhibiting activities a ...201424980018
listeria monocytogenes dose response revisited--incorporating adjustments for variability in strain virulence and host susceptibility.evaluations of listeria monocytogenes dose-response relationships are crucially important for risk assessment and risk management, but are complicated by considerable variability across population subgroups and l. monocytogenes strains. despite difficulties associated with the collection of adequate data from outbreak investigations or sporadic cases, the limitations of currently available animal models, and the inability to conduct human volunteer studies, some of the available data now allow r ...201524975545
mycobacterium tuberculosis rv1096 protein: gene cloning, protein expression, and peptidoglycan deacetylase activity.many bacteria modulate and evade the immune defenses of their hosts through peptidoglycan (pg) deacetylation. the pg deacetylases from streptococcus pneumonia, listeria monocytogenes and lactococcus lactis have been characterized. however, thus far, the pg deacetylase of mycobacterium tuberculosis has not been identified.201424975018
the surface protein lmo1941 with lysm domain influences cell wall structure and susceptibility of listeria monocytogenes to cephalosporins.listeria monocytogenes is a gram-positive bacterium causing rare but dangerous cases of disease in humans and animals. the β-lactams penicillin g and ampicillin are the antibiotics of choice in the treatment of listeriosis. recently, lmo1941, encoding a surface protein of l. monocytogenes with unknown function, was identified as a gene transcriptionally upregulated under penicillin g pressure. in this study, the effect of lmo1941 knockout on the susceptibility of l. monocytogenes to β-lactams wa ...201424974853
visual and sensitive detection of viable pathogenic bacteria by sensing of rna markers in gold nanoparticles based paper platform.food-borne pathogens have been recognized as a major cause of human infections worldwide. their identification needs to be simpler, cheaper and more reliable than the traditional methods. here, we constructed a low-cost paper platform for viable pathogenic bacteria detection with the naked eye. in this study, an effective isothermal amplification method was used to amplify the hlya mrna gene, a specific rna marker in listeria monocytogenes. the amplification products were applied to the paper-ba ...201424973541
pregnancy-associated listeriosis: clinical characteristics and geospatial analysis of a 10-year period in israel.listeria monocytogenes is a foodborne pathogen that causes life-threatening infections in elderly, immunocompromised, and pregnant women. in pregnancy it may cause fetal loss or a preterm delivery, and the neonate is prone to neonatal sepsis and death.201424973315
proinflammatory and phagocytic functions of alveolar macrophages in obesity. 200924973149
evaluation of the removal and destruction effect of a chlorine and thiamine dilaurylsulfate combined treatment on l. monocytogenes biofilm.the present study investigated the efficacy of single and combined treatment of both chlorine and thiamine dilaurylsulfate (tds) on the reduction of listeria monocytogenes biofilms in microtiter plate. the disinfectants used in this study were 50, 100, and 200 mg/l chlorine and 100, 500, and 1000 mg/l of tds. biofilm-forming index (bfi) and culturable cell count were used to evaluate the disinfectant assay. the highest bfi reduction was 0.80, achieved by the combination of 200 mg/l chlorine and ...201424971770
listeria monocytogenes induces ifnβ expression through an ifi16-, cgas- and sting-dependent pathway.listeria monocytogenes is a gram-positive facultative intracellular bacterium, which replicates in the cytoplasm of myeloid cells. interferon β (ifnβ) has been reported to play an important role in the mechanisms underlying listeria disease. although studies in murine cells have proposed the bacteria-derived cyclic-di-amp to be the key bacterial immunostimulatory molecule, the mechanism for ifnβ expression during l. monocytogenes infection in human myeloid cells remains unknown. here we report t ...201424970844
an unusual presentation of listeriosis: anemia and cutaneous manifestations.listeria monocytogenes is an intracellular pathogen causing food-borne disease. it usually affects the young as well as immunocompromised individuals and is associated with high mortality rates. cutaneous manifestations have rarely been described. we describe an interesting case of a traveller from the tropics presenting with cutaneous listeriosis and anemia.201424968680
effect of yoghurt or yoghurt serum on microbial quality of cig kofte.cig kofte, raw meatball is a traditionally produced meat product in turkey and some other middle east countries. it is prepared from mixtures of finely minced raw beef, bulgur, onions, various spices and tap water. cig kofte is an uncooked product and popularly consumed with lettuce and lemon juice. in this study, yoghurt or yoghurt serum (ys) were added to the mixtures of cig kofte instead of tap water to reduce microbial risks of the raw meatball. additionally, the effects of yoghurt and ys on ...201424966438
misidentification of listeria monocytogenes by the vitek 2 system. 201424966361
colorimetric paper-based detection of escherichia coli, salmonella spp., and listeria monocytogenes from large volumes of agricultural water.this protocol describes rapid colorimetric detection of escherichia coli, salmonella spp., and listeria monocytogenes from large volumes (10 l) of agricultural waters. here, water is filtered through sterile modified moore swabs (mms), which consist of a simple gauze filter enclosed in a plastic cartridge, to concentrate bacteria. following filtration, non-selective or selective enrichments for the target bacteria are performed in the mms. for colorimetric detection of the target bacteria, the e ...201424962090
listeria monocytogenes brainstem infection (rhombencephalitis) mimicking ischemic stroke.the frequency of listeria monocytogenes (lm) infection of the central nervous system is increasing. we report a patient recently treated with chemotherapeutic drugs for pulmonary adenocarcinoma who suddenly developed hemiparesis, was initially diagnosed with stroke, and was then found to be affected by lm rhombencephalitis accompanied by a brain abscess. lm meningoencephalitis mimicking ischemic stroke is rare but must be considered, especially in specific patients.201424961733
transfusion-related listeria monocytogenes infection in a patient with acute myeloid leukaemia. 201424960659
listeria monocytogenes-associated meningitis and arthritis in an immunocompetent 65-year-old woman: a case report.we report an unusual case of concurrent meningitis and infectious monoarthritis due to listeria monocytogenes in a 65-year-old woman presenting to our department with a transient gastrointestinal tract illness. during hospitalization the patient's neurological status deteriorated, presenting signs of meningeal irritation along with signs of inflammation and oedema of the right knee. blood cultures and cultures of the cerebrospinal fluid and of the sinovial fluid aspirate showed growth of l. mono ...201424955800
soft cheese supplemented with black cumin oil: impact on food borne pathogens and quality during storage.black cumin seed oil (bcso) was tested for its inhibitory effect against some pathogenic bacteria (staphylococcus aureus atcc 6538, escherichia coli atcc 8739, listeria monocytogenes scott a and salmonella enteritidis pt4) in domiati cheese during cold storage. physical, chemical and sensorial changes in cheese during storage were recorded. pasteurized milk was inoculated before renneting with a mixed culture of bacteria at ca. 4 log cfu ml(-1). in vitro and in situ supplementation with bcso sho ...201424955014
mycotic abdominal aortic aneurysm from listeria monocytogenes. 201424954294
helminth infections predispose mice to pneumococcal pneumonia but not to other pneumonic pathogens.pneumonia is the leading killer of children worldwide. here, we report that helminth-infected mice develop fatal pneumonia when challenged with streptococcus pneumoniae. mice were chronically infected with either the flatworm taenia crassiceps or the roundworm heligmosomoides polygyrus. upon challenge with a pneumonic type 3 strain of s. pneumoniae (a66.1), the worm-infected mice developed pneumonia at a rate and to a degree higher than age-matched control mice as measured by bioluminescent imag ...201424952091
genetic characterization of plasmid-associated triphenylmethane reductase in listeria monocytogenes.the enzyme triphenylmethane reductase (tmr) reduces toxic triphenylmethane dyes into colorless, nontoxic derivatives, and tmr-producing microorganisms have been proposed as bioremediation tools. analysis of the genome of listeria monocytogenes h7858 (1998-1999 hot dog outbreak) revealed that the plasmid (plm80) of this strain harboring a gene cassette (bcrabc) conferring resistance to benzalkonium chloride (bc) and other quaternary ammonium disinfectants also harbored a gene (tmr) highly homolog ...201424951782
the polycomb repressive complex 2 governs life and death of peripheral t cells.differentiation of naïve cd4(+) t cells into effector (th1, th2, and th17) and induced regulatory (itreg) t cells requires lineage-specifying transcription factors and epigenetic modifications that allow appropriate repression or activation of gene transcription. the epigenetic silencing of cytokine genes is associated with the repressive h3k27 trimethylation mark, mediated by the ezh2 or ezh1 methyltransferase components of the polycomb repressive complex 2 (prc2). here we show that silencing o ...201424951427
extraction, antioxidant and antilisterial activities of polysaccharides from the flower of viper's bugloss.the objective of the present research was to investigate the effect of microwave-assisted extraction (mae) conditions on the extraction yield, antioxidant properties and antilisterial activities of the polysaccharides from the flowers of viper's bugloss (echium vulgare l.). the four extraction variables, time (40-100min), microwave power (200-800w), temperature (30-70°c), and the ratio of water to raw material (10-70), were optimized using response surface methodology. the experimental data were ...201424950309
a fast, reliable, and sensitive method for detection and quantification of listeria monocytogenes and escherichia coli o157:h7 in ready-to-eat fresh-cut products by mpn-qpcr.in the present work we developed a mpn quantitative real-time pcr (mpn-qpcr) method for a fast and reliable detection and quantification of listeria monocytogenes and escherichia coli o157:h7 in minimally processed vegetables. in order to validate the proposed technique, the results were compared with conventional mpn followed by phenotypic and biochemical assays methods. when l. monocytogenes and e. coli o157:h7 were artificially inoculated in fresh-cut vegetables, a concentration as low as 1 c ...201424949460
use carum copticum essential oil for controlling the listeria monocytogenes growth in fish model system.this study was conducted to evaluate the antibacterial effect of carum copticum essential oil (ajowan eo) against listeria monocytogenes in fish model system. ajowan eo chemical composition was determined by gas chromatography/mass spectral analysis and the highest concentration of carum copticum essential oil without any significant changes on sensory properties of kutum fish (rutilus frisii kutum) was assigned. then the inhibitory effect of ajowan eo at different concentrations in presence of ...201424948918
control of listeria monocytogenes growth in soft cheeses by bacteriophage p100.the purpose of this study was to determine the effect of bacteriophage p100 on strains of listeria monocytogenes in artificially inoculated soft cheeses. a mix of l. monocytogenes 1/2a and scott a was inoculated in minas frescal and coalho cheeses (approximately 10(5) cfu/g) with the bacteriophage added thereafter (8.3 × 10(7) pfu/g). samples were analyzed immediately, and then stored at 10 °c for seven days. at time zero, 30 min post-infection, the bacteriophage p100 reduced l. monocytogenes co ...201424948908
role of host gtpases in infection by listeria monocytogenes.the bacterial pathogen listeria monocytogenes induces internalization into mammalian cells and uses actin-based motility to spread within tissues. listeria accomplishes this intracellular life cycle by exploiting or antagonizing several host gtpases. internalization into human cells is mediated by the bacterial surface proteins inla or inlb. these two modes of uptake each require a host actin polymerization pathway comprised of the gtpase rac1, nucleation promotion factors, and the arp2/3 comple ...201424948362
toxoplasma gondii profilin promotes recruitment of ly6chi ccr2+ inflammatory monocytes that can confer resistance to bacterial infection.ly6c+ inflammatory monocytes are essential to host defense against toxoplasma gondii, listeria monocytogenes and other infections. during t. gondii infection impaired inflammatory monocyte emigration results in severe inflammation and failure to control parasite replication. however, the t. gondii factors that elicit these monocytes are unknown. early studies from the remington laboratory showed that mice with a chronic t. gondii infection survive lethal co-infections with unrelated pathogens, i ...201424945711
antibacterial, antiviral, and antifungal properties of wines and winery byproducts in relation to their flavonoid content.grapes produce organic compounds that may be involved in the defense of the plants against invading phytopathogens. these metabolites include numerous phenolic compounds that are also active against human pathogens. grapes are used to produce a variety of wines, grape juices, and raisins. grape pomace, seeds, and skins, the remains of the grapes that are a byproduct of winemaking, also contain numerous bioactive compounds that differ from those found in grapes and wines. this overview surveys an ...201424945318
toxin production and growth of pathogens subjected to temperature fluctuations simulating consumer handling of cold cuts.it is crucial for the quality and safety of ready-to-eat (rte) foods to maintain the cold chain from production to consumption. the effect of temperature abuse related to daily meals and elevated refrigerator temperatures on the growth and toxin production of bacillus cereus, bacillus weihenstephanensis and staphylococcus aureus and the growth of listeria monocytogenes and yersinia enterocolitica was studied. a case study with temperature loggings in the domestic environment during easter and ch ...201424935689
listeria exploits damage and death to spread bad news.pathogenic listeria monocytogenes replicates within the host cytosol; little is known about how it transits from cell to cell, spreading infection. a recent study implicates infection-induced membrane damage as a trigger for efferocytosis, the recognition and uptake of dead cells, thereby tricking neighboring cells into taking up the invader.201424934861
[bacteriostatic effect and/or xylitol bactericide of crops on listeria monocytogenes].listeria monocytogenes has been considered as an emerging pathogen causing foodborne illness. in the search for an alternate route biocontrol propagation, xylitol has been proposed as a possible bacteriostatic and / or bactericide. xylitol is a polyol derived from the hydrogenation of xylose monosaccharide of importance in the pharmaceutical industry for its anti-cariogenic effect. to check the possible effect of xylitol as bacteriostatic and/or bactericidal against listeria monocytogenes, it wa ...201324934074
development of 23 individual taqman® real-time pcr assays for identifying common foodborne pathogens using a single set of amplification conditions.most of the acute intestinal diseases are caused by foodborne pathogens with infants and elderly people being at major risk. the aim of this study was to develop a procedure to simultaneously detect 20 foodborne pathogens in complex alimentary matrices such as milk, cheese and meat. the list of targets include, among the others, listeria spp., salmonella spp., shigella spp., escherichia coli spp., campylobacter spp., clostridium spp. and staphylococcus aureus. the accuracy of detection was deter ...201424929880
atmospheric cold plasma inactivation of escherichia coli, salmonella enterica serovar typhimurium and listeria monocytogenes inoculated on fresh produce.atmospheric cold plasma (acp) represents a potential alternative to traditional methods for non-thermal decontamination of foods. in this study, the antimicrobial efficacy of a novel dielectric barrier discharge acp device against escherichia coli, salmonella enterica typhimurium and listeria monocytogenes inoculated on cherry tomatoes and strawberries, was examined. bacteria were spot inoculated on the produce surface, air dried and sealed inside a rigid polypropylene container. samples were in ...201424929725
listeria ivanovii atcc 19119 strain behaviour is modulated by iron and acid stress.it has been suggested that the rarity of human listeriosis due to listeria ivanovii reflects not only host tropism factors but also the rare occurrence of this species in the environment, compared with listeria monocytogenes. in the present study we evaluate the effects on the reference strain l. ivanovii atcc 19119 behaviour of two combined stresses, low iron availability and acid environment, that bacteria can encounter in the passage from saprophytic life to the host. in these conditions, l.  ...201424929719
antimicrobial resistance of listeria monocytogenes and listeria innocua from meat products and meat-processing environment.a total of 336 listeria isolates from ready-to-eat (rte) meat products and meat-processing environments, consisting of 206 listeria monocytogenes, and 130 listeria innocua isolates, were characterized by disc diffusion assay and minimum inhibitory concentration (mic) values for antimicrobial susceptibility against twenty antimicrobials. resistance to one or two antimicrobials was observed in 71 l. monocytogenes isolates (34.5%), and 56 l. innocua isolates (43.1%). multidrug resistance was identi ...201424929718
evaluation of indirect impedance for measuring microbial growth in complex food matrices.the suitability of indirect impedance to accurately measure microbial growth in real food matrices was investigated. a variety of semi-solid and liquid food products were inoculated with bacillus cereus, listeria monocytogenes, staphylococcus aureus, lactobacillus plantarum, pseudomonas aeruginosa, escherichia coli, salmonella enteriditis, candida tropicalis or zygosaccharomyces rouxii and co2 production was monitored using a conductimetric (don whitely r.a.b.i.t.) system. the majority (80%) of ...201424929710
synergistic effects of ultrasound and sodium hypochlorite (naocl) on reducing listeria monocytogenes atcc19118 in broth, stainless steel, and iceberg lettuce.this study was performed in order to determine whether a combined treatment of ultrasound and sodium hypochlorite (naocl) is more effective than individual treatment on reducing listeria monocytogenes atcc19118 on stainless steel and iceberg lettuce. the bactericidal effect of ultrasound and naocl was investigated in tryptic soy broth (tsb), on stainless steel and iceberg lettuce. various concentrations of naocl (50, 100, 150, and 200 ppm) were tested along with various ultrasound treatment time ...201424927252
a single exposure to a sublethal pediocin concentration initiates a resistance-associated temporal cell envelope and general stress response in listeria monocytogenes.listeria monocytogenes can cause the potentially fatal food-borne disease listeriosis, and the use of bacteriocin-producing lactic acid bacteria to control l. monocytogenes holds great promise. however, the development of bacteriocin resistance is a potential challenge, and the purpose of this study was to determine if exposure to sublethal concentrations of pediocin-containing lactobacillus plantarum whe 92 supernatant could prime l. monocytogenes for resistance. by transcriptomic analysis, we ...201524920558
building a molecular listeria monocytogenes database to centralize and share pfge typing data from food, environmental and animal strains throughout europe.the european union reference laboratory (eurl) for listeria monocytogenes (lm) collaborates with a network of 35 national reference laboratories (nrls) throughout europe. most of these nrls are in charge of detecting and typing lm strains from food, environment and animals, which are isolated nationally. the past few years eurl activities have enabled nrls to reinforce typing capabilities according to standardised protocols. consequently the need to exchange typing data within the nrl network ha ...201424918990
establishment of a simple and rapid identification method for listeria spp. by using high-resolution melting analysis, and its application in food industry.listeria monocytogenes is the causative bacteria of listeriosis, which has a higher mortality rate than that of other causes of food poisoning. listeria spp., of which l. monocytogenes is a member, have been isolated from food and manufacturing environments. several methods have been published for identifying listeria spp.; however, many of the methods cannot identify newly categorized listeria spp. additionally, they are often not suitable for the food industry, owing to their complexity, cost, ...201424918440
sting ligand c-di-gmp improves cancer vaccination against metastatic breast cancer.cancer vaccination may be our best and most benign option for preventing or treating metastatic cancer. however, breakthroughs are hampered by immune suppression in the tumor microenvironment. in this study, we analyzed whether cyclic diguanylate (c-di-gmp), a ligand for stimulator of interferon genes (sting), could overcome immune suppression and improve vaccination against metastatic breast cancer. mice with metastatic breast cancer (4t1 model) were therapeutically immunized with an attenuated ...201424913717
radiation-treated ready-to-eat (rte) chicken breast adobo for immuno-compromised patients.usually in hospitals low-bacterial diets are served to immuno-compromised patients (icps). however, low-bacterial diets still pose a high risk of microbial infections and limit the food selection of the patients. thus, pathogen-free dishes must be made available. this study presents the development of pathogen-free ready-to-eat (rte) filipino ethnic food chicken breast adobo, sterilized by exposure to high-dose gamma rays (25 kgy) in combination with conventional treatments. frozen vacuum-packed ...201424912709
enhanced survival during experimental listeria monocytogenes sepsis in neonatal mice prophylactically treated with th1 and macrophage immunoregulatory cytokines and mediators.impairments in t-cell and macrophage-mediated host defense lead to increased infection-related morbidity and mortality in neonates, partly because of immaturity in t helper (th)1 function. listeria monocytogenes (lm) is an intracellular pathogen disproportionately causing severe disease among neonates and the immunocompromised. intact macrophage and th1-mediated immune responses are critical for lm clearance. we and others have previously demonstrated downregulation of th1 and macrophage immunor ...201424911893
a trip in the "new microbiology" with the bacterial pathogen listeria monocytogenes.listeria monocytogenes is a food-borne pathogen causing an opportunistic disease called listeriosis. this bacterium invades and replicates in most cell types, due to its multiple strategies to exploit host molecular mechanisms. research aiming at unravelling listeria invasion and intracellular lifestyle has led to a number of key discoveries in infection biology, cell biology and also microbiology. in this review, we report on our most recent advances in understanding the intimate crosstalk betw ...201424911203
congenic mice reveal genetic epistasis and overlapping disease loci for autoimmune diabetes and listeriosis.the nonobese diabetic (nod) mouse strain serves as a genomic standard for assessing how allelic variation for insulin-dependent diabetes (idd) loci affects the development of autoimmune diabetes. we previously demonstrated that c57bl/6 (b6) mice harbor a more diabetogenic allele than nod mice for the idd14 locus when introduced onto the nod genetic background. new congenic nod mouse strains, harboring smaller b6-derived intervals on chromosome 13, now localize idd14 to an ~18-mb interval and rev ...201424906421
cytotoxic cells kill intracellular bacteria through granulysin-mediated delivery of granzymes.when killer lymphocytes recognize infected cells, perforin delivers cytotoxic proteases (granzymes) into the target cell to trigger apoptosis. what happens to intracellular bacteria during this process is unclear. human, but not rodent, cytotoxic granules also contain granulysin, an antimicrobial peptide. here, we show that granulysin delivers granzymes into bacteria to kill diverse bacterial strains. in escherichia coli, granzymes cleave electron transport chain complex i and oxidative stress d ...201424906149
enhanced bactericidal action of acidified sodium chlorite caused by the saturation of reactants.factors affecting the antibacterial action of acidified sodium chlorite (asc), a widely used disinfectant, have not been determined. this study investigated the significant factors suggesting efficient production method to maximize bactericidal action of asc.201424905216
cell-wall deficient l. monocytogenes l-forms feature abrogated pathogenicity.stable l-forms are cell wall-deficient bacteria which are able to multiply and propagate indefinitely, despite the absence of a rigid peptidoglycan cell wall. we investigated whether l-forms of the intracellular pathogen l. monocytogenes possibly retain pathogenicity, and if they could trigger an innate immune response. while phagocytosis of l. monocytogenes l-forms by non-activated macrophages sometimes resulted in an unexpected persistence of the bacteria in the phagocytes, they were effective ...201424904838
listeria monocytogenes meningoencephalitis in adults: analysis of factors related to unfavourable outcome.to analyse the short-term outcome in patients with listeria monocytogenes meningoencephalitis (lmme) to improve management and outcome.201424902522
effect of cinnamon (cinnamomum zeylanicum) essential oil supplementation on lamb growth performance and meat quality characteristics.a trial was conducted to examine the effect of cinnamon essential oil supplementation on lamb growth performance and meat quality. sixteen male lambs were randomly assigned to two groups. the first group served as control and was given a basal diet, and the second group was given the same diet supplemented with cinnamon oil (1 ml/kg of concentrated feed) for 35 days. incorporation of cinnamon oil did not affect growth performance (p>0.05). meat ph, colour, water-holding capacity, shear force, in ...201424902083
quantum dots and graphene oxide fluorescent switch based multivariate testing strategy for reliable detection of listeria monocytogenes.graphene oxide (go) and quantum dots (qds), as burgeoning types of nanomaterials, have gained tremendous interest in the biosensor field. in this work, we designed a novel multivariate testing strategy that depends on the fluorescence resonance energy transfer (fret) effect between quantum dots (qds) and graphene oxide (go). it integrates the qd-go fret principle and qd probes with different emission peaks into a platform, aims at multiplex gene detection of a human infectious and highly pathoge ...201424901065
dietary pyridoxine controls efficacy of vitamin b6-auxotrophic tuberculosis vaccine bacillus calmette-guérin δurec::hly δpdx1 in mice.the only tuberculosis (tb) vaccine in use today, bacillus calmette-guérin (bcg), provides insufficient protection and can cause adverse events in immunocompromised individuals, such as bcgosis in hiv(+) newborns. we previously reported improved preclinical efficacy and safety of the recombinant vaccine candidate bcg δurec::hly, which secretes the pore-forming listeriolysin o of listeria monocytogenes. here, we evaluate a second-generation construct, bcg δurec::hly δpdx1, which is deficient in py ...201424895310
bactericidal thurincin h causes unique morphological changes in bacillus cereus f4552 without affecting membrane permeability.thurincin h is an antilisterial bacteriocin produced by bacillus thuringiensis sf361. it exhibits inhibitory activity against a wide range of gram-positive foodborne pathogens and spoilage bacteria including listeria monocytogenes, b. cereus, and b. subtilis. this hydrophobic, anionic bacteriocin folds into a hairpin structure maintained by four pairs of unique sulfur to α-carbon thioether bonds. as its hydrophobicity and structure are quite different from most archived bacteriocins, this study ...201424891232
magnetostrictive particle based biosensors for in situ and real-time detection of pathogens in water.biosensors for in situ detection of pathogenic bacteria in liquid are developed using magnetostrictive particles (msp) as the sensor platform. the sensing elements used are phage e2 against salmonella typhimurium, monoclonal antibody against listeria monocytogenes, polyclonal antibody against escherichia coli, and polyclonal antibody against staphylococcus aureus, respectively. these biosensors were characterized in cultures with different populations ranging from 5 × 10(1) to 5 × 10(8)  cfu/ml. ...201424890794
classification of listeria monocytogenes persistence in retail delicatessen environments using expert elicitation and machine learning.increasing evidence suggests that persistence of listeria monocytogenes in food processing plants has been the underlying cause of a number of human listeriosis outbreaks. this study extracts criteria used by food safety experts in determining bacterial persistence in the environment, using retail delicatessen operations as a model. using the delphi method, we conducted an expert elicitation with 10 food safety experts from academia, industry, and government to classify l. monocytogenes persiste ...201424888445
cultivated strains of agaricus bisporus and a. brasiliensis: chemical characterization and evaluation of antioxidant and antimicrobial properties for the final healthy product--natural preservatives in yoghurt.agaricus bisporus (j. e. lange) emil j. imbach and agaricus brasiliensis wasser, m. didukh, amazonas & stamets are edible mushrooms. we chemically characterized these mushrooms for nutritional value, hydrophilic and lipophilic compounds. the antioxidant and antimicrobial activities of methanolic and ethanolic extracts were assessed. hepatotoxicity was also evaluated. the ethanolic extract of both species was tested for inhibition of listeria monocytogenes growth in yoghurt. both species proved t ...201424881564
combined effects of chlorine and thiamine dilauryl sulfate on reduction of listeria monocytogenes in chicken breast and development of predictive growth models.the inhibitory effect of chlorine (50, 100, and 200 ml/kg) and thiamine dilauryl sulfate (tds: 100, 500, and 1,000 mg/kg) on listeria monocytogenes in chicken breast was investigated. also, predictive growth models as a function of chlorine and tds concentration, and storage temperature (4, 10, and 15°c) were developed using a polynomial model. listeria monocytogenes counts were significantly (p < 0.05) different in samples treated with sterile distilled water and combinations of chlorine and td ...201424879700
biosynthesis of silver nanoparticles using acacia leucophloea extract and their antibacterial activity.the immense potential of nanobiotechnology makes it an intensely researched field in modern medicine. green nanomaterial synthesis techniques for medicinal applications are desired because of their biocompatibility and lack of toxic byproducts. we report the toxic byproducts free phytosynthesis of stable silver nanoparticles (agnps) using the bark extract of the traditional medicinal plant acacia leucophloea (fabaceae). visual observation, ultraviolet-visible spectroscopy, and transmission elect ...201424876776
microwave-assisted hydrodistillation of essential oil from rosemary.effects of microwave assisted hydrodistillation (mahd) and conventional hydrodistillation (hd) methods on yield, composition, specific gravity, refractive index, and antioxidant and antimicrobial activities of essential oil of rosmarinus officinalis l were studied. the main aroma compounds of rosemary essential oil were found as 1,8-cineole and camphor. trolox equivalent antioxidant capacity (teac) values for essential oils extracted by mahd and hd were 1.52 mm/ml oil and 1.95 mm/ml oil, respect ...201424876637
episomal expression of truncated listeriolysin o in lmdda-llo-e7 vaccine enhances antitumor efficacy by preferentially inducing expansions of cd4+foxp3- and cd8+ t cells.studies have shown that listeria monocytogenes (lm)-based vaccine expressing a fusion protein comprising truncated listeriolysin o (llo) and human papilloma virus (hpv) e7 protein (lm-llo-e7) induces a decrease in regulatory t cells (treg) and complete regression of established, transplanted hpv-tc-1 tumors in mice. however, how the lm-based vaccine causes a decrease in tregs remains unclear. using a highly attenuated lm dal dat δacta strain (lmdda)-based vaccine, we report here that the vector ...201424872025
[rapid detection of listeria monocytogenes in pork samples by real-time pcr with taqman probe].to develop a real-time pcr method for detection listeria monocytogenes in pork samples.201424868965
selective pharmacologic inhibition of a pasta kinase increases listeria monocytogenes susceptibility to β-lactam antibiotics.while β-lactam antibiotics are a critical part of the antimicrobial arsenal, they are frequently compromised by various resistance mechanisms, including changes in penicillin binding proteins of the bacterial cell wall. genetic deletion of the penicillin binding protein and serine/threonine kinase-associated protein (pasta) kinase in methicillin-resistant staphylococcus aureus (mrsa) has been shown to restore β-lactam susceptibility. however, the mechanism remains unclear, and whether pharmacolo ...201424867981
attenuated listeria monocytogenes: a powerful and versatile vector for the future of tumor immunotherapy.for over a century, inactivated or attenuated bacteria have been employed in the clinic as immunotherapies to treat cancer, starting with the coley's vaccines in the 19th century and leading to the currently approved bacillus calmette-guérin vaccine for bladder cancer. while effective, the inflammation induced by these therapies is transient and not designed to induce long-lasting tumor-specific cytolytic t lymphocyte (ctl) responses that have proven so adept at eradicating tumors. therefore, in ...201424860789
numerical spatio-temporal characterization of listeria monocytogenes biofilms.as the structure of biofilms plays a key role in their resistance and persistence, this work presents for the first time the numerical characterization of the temporal evolution of biofilm structures formed by three listeria monocytogenes strains on two types of stainless-steel supports, aisi 304 ss no. 2b and aisi 316 ss no. 2r. counting methods, motility tests, fluorescence microscopy and image analysis were combined to study the dynamic evolution of biofilm formation and structure. image anal ...201424858448
selection and characterization of dna aptamers specific for listeria species.single-stranded (ss) dna aptamers with binding affinity to listeria spp. were selected using a whole-cell selex (systematic evolution of ligands by exponential enrichment) method. listeria monocytogenes cells were grown at 37°c and harvested at mid-log phase or early stationary phase to serve as the targets in selex. a total of 10 unique aptamer sequences were identified, six associated with log phase cells and four with stationary phase cells. binding affinity of the aptamers was determined usi ...201424857773
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