Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| [results of epidemiological surveillance of hospital infections at the clinic of digestive system surgery, ccs, in 2007]. | nosocomial infections (ni) are significant medical problem in the countries worldwide. ni significance reflects in higher morbidity and mortality rates, and moreover, nis add to longer stay and higher treatment costs. based on data obtained from underdeveloped and developing countries, over 20% of hospitalized patients acquire some of nis, while that proportion is 5% in developed countries. | 2009 | 19780330 |
| performance of vitek-2 compact and overnight microscan panels for direct identification and susceptibility testing of gram-negative bacilli from positive fan bact/alert blood culture bottles. | we describe the reliability of the vitek-2 compact and overnight microscan panels for direct identification and susceptibility testing from the bact/alert blood culture system when using fan (fa and fn) bottles. a simple procedure, in two centrifugation steps, was designed to remove the charcoal particles present in fa and fn bottles. a total of 113 positive blood cultures showing gram-negative rods were investigated. enterobacteriaceae were isolated in 104 cases, and pseudomonas aeruginosa in n ... | 2010 | 19778301 |
| planktonic and biofilm communities from 7-day-old chicken cecal microflora cultures: characterization and resistance to salmonella colonization. | information implicating bacterial biofilms as contributory factors in the development of environmental bacterial resistance has been increasing. there is a lack of information regarding the role of biofilms within the microbial ecology of the gastrointestinal tract of food animals. this work used a continuous-flow chemostat model derived from the ceca of 7-day-old chicks to characterize these communities and their ability to neutralize invasion by salmonella enterica serovar typhimurium. we char ... | 2009 | 19777880 |
| nosocomial transmission of highly resistant microorganisms on a spinal cord rehabilitation ward. | to study the mechanism of nosocomial transmission of highly resistant microorganisms (hrmos). | 2009 | 19777864 |
| change in cochlear response in an animal model of otitis media with effusion. | our previous studies confirm that middle ear mobility is reduced in the presence of otitis media with effusion (ome). variations in middle ear function may result in changes in cochlear response in ome ears. with the long-term goal of evaluating cochlear function in ome ears, the aim of this study was to measure the displacement of the basilar membrane (bm) in guinea pig ears with ome. vibrations of the bm at the apex and basal turn were measured in an in vitro preparation extracted 3 and 14 day ... | 2010 | 19776565 |
| [sustainable electricity generation in microbial fuel cells using fe(iii)-edta as cathodic electron shuttle]. | the rate of oxygen reduction reaction (orr) at the cathode is a major factor affecting the performance of the microbial fuel cells (mfc). results showed that when using fe(iii)-edta solution as catholyte, the orr rate was significantly increased and the internal resistance was reduced, consequently leading to an increase in power output. with a concentration of fe(iii )-edta at 20.0 mmol/l in the catholyte, the mfc produced the voltage and power density at approximate 200.1 mv and 16.0 mw/m2, re ... | 2009 | 19775022 |
| a k2a-positive klebsiella pneumoniae causes liver and brain abscess in a saint kitt's man. | klebsiella pneumoniae isolated in community-acquired pneumonia is increasingly found in primary pyogenic liver abscesses. the presence of maga in k. pneumoniae has been implicated in hypermucoviscosity and virulence of liver abscess isolates. the k2 serotype has also been strongly associated with hypervirulence. we report the isolation of non-maga, k2 k. pneumoniae strain from a liver abscess of a saint kitt's man who survived the invasive syndrome. | 2009 | 19774200 |
| pneumonia in severely malnourished children in developing countries - mortality risk, aetiology and validity of who clinical signs: a systematic review. | to quantify the degree by which moderate and severe degrees of malnutrition increase the mortality risk in pneumonia, to identify potential differences in the aetiology of pneumonia between children with and without severe malnutrition, and to evaluate the validity of who-recommended clinical signs (age-specific fast breathing and chest wall indrawing) for the diagnosis of pneumonia in severely malnourished children. | 2009 | 19772545 |
| klebsiella pneumoniae endophthalmitis secondary to liver abscess presenting as acute iridocyclitis. | a 51-year-old man presented with acute iridocyclitis with no evidence of vitritis on b-scan that progressed to endogenous endophthalmitis. systemic work-up revealed a large liver abscess. urine, blood, liver abscess, and vitreous aspirate cultures all grew klebsiella pneumoniae. the eye was enucleated secondary to severe pain from neovascular glaucoma and loss of vision. | 2009 | 19772282 |
| is there an association between nosocomial infection rates and bacterial cross transmissions? | surveillance data of nosocomial infection rates are increasingly used for public reporting and interhospital comparisons. approximately 15% of nosocomial infections on intensive care units are the result of patient-to-patient transmissions of the causative organisms. these exogenous infections could be prevented by adherence to basic infection control measures. the association between bacterial cross transmissions and nosocomial infection rates was analyzed. | 2010 | 19770743 |
| achn-490, a neoglycoside with potent in vitro activity against multidrug-resistant klebsiella pneumoniae isolates. | the in vitro activity of achn-490, a novel aminoglycoside ("neoglycoside"), was evaluated against 102 multidrug-resistant (mdr) klebsiella pneumoniae strains, including a subset of 25 strains producing the kpc carbapenemase. mic50 values for gentamicin, tobramycin, and amikacin were 8 microg/ml, 32 microg/ml, and 2 microg/ml, respectively; mic90 values for the same antimicrobials were > or = 64 microg/ml, > or = 64 microg/ml, and 32 microg/ml, respectively. achn-490 showed an mic50 of 0.5 microg ... | 2009 | 19770287 |
| characterization of a new metallo-beta-lactamase gene, bla(ndm-1), and a novel erythromycin esterase gene carried on a unique genetic structure in klebsiella pneumoniae sequence type 14 from india. | a swedish patient of indian origin traveled to new delhi, india, and acquired a urinary tract infection caused by a carbapenem-resistant klebsiella pneumoniae strain that typed to the sequence type 14 complex. the isolate, klebsiella pneumoniae 05-506, was shown to possess a metallo-beta-lactamase (mbl) but was negative for previously known mbl genes. gene libraries and amplification of class 1 integrons revealed three resistance-conferring regions; the first contained bla(cmy-4) flanked by isec ... | 2009 | 19770275 |
| antimicrobial and antioxidant activities of plants from northeast of mexico. | traditional medicine has a key role in health care worldwide. obtaining scientific information about the efficacy and safety of the plants from our region is one of the goals of our research group. in this report, 17 plants were selected and collected in different localities from northeast mexico. the dried plants were separated into leaves, flowers, fruit, stems, roots and bark. each part was extracted with methanol, and 39 crude extracts were prepared. the extracts were tested for their antimi ... | 2009 | 19770266 |
| [endogenous klebsiella pneumoniae endophthalmitis secondary to a renal abscess: a case report]. | we report the case of an 86-year-old man with unilateral panuveitis concurrent with an infectious retinal focus. the diagnosis of endogenous endophthalmitis with subretinal abscess was suggested. the biology workup and ct scan showed a renal abscess. this was caused by klebsiella pneumoniae, which was confirmed by analysis of abscess aspiration and vitrectomy samples. this paper underscores the importance of etiological assessment in infectious panuveitis and the consequences on visual outcome. | 2009 | 19769860 |
| molecular epidemiology of extended-spectrum beta-lactamase-producing klebsiella pneumoniae strains in a university hospital in tunis, tunisia, 1999-2005. | during a period of 6 years and 5 months (january 1999 to may 2005), 103 extended-spectrum beta-lactamase (esbl)-producing klebsiella pneumoniae isolates, each from an individual patient or site, were collected at mongi slim university hospital centre, tunis, tunisia. the objectives of our work were the characterization of the bla genes encoding esbls, the investigation of clonal diversity of strains, and identification of the transmission modes of the resistance genes. we carried out detection b ... | 2010 | 19769601 |
| chemical composition and antibacterial activity of the essential oil of cordia verbenacea from the venezuelan andes. | the essential oil of cordia verbenacea d.c. (boraginaceae) that grows in mérida-venezuela was obtained by hydrodistillation from the aerial parts of the plant, yielding 0.21%. the oil was analyzed by gc-fid and gc-ms. thirty one components which made up 94.3% of the oil were identified. the most abundant constituents found were: tricyclene (23.9%), bicyclogermacrene (11.7%), germacrene d (9.9%) and beta-caryophyllene (8.2%). antibacterial activity determination was carried out according to the d ... | 2009 | 19768996 |
| aetiology of bacteraemia among adult aids patients attending lagos university teaching hospital (luth), lagos, nigeria. | bacteraemia is a relatively common event in hiv-infected patients, especially in late infection. studies in africa have shown that more than 23% of aids patients have bacteraemia but there is paucity of data from nigeria. | 2009 | 19767904 |
| purple urine bag syndrome. | 2009 | 19767663 | |
| hepatocellular carcinoma associated with liver abscess. | hepatitis b and hepatitis c are highly prevalent in taiwan. chronic hepatitis patients are at high risk of progression to liver cirrhosis and hepatocellular carcinoma (hcc). however, hcc in association with liver abscess is very rare. accordingly, this study analyzed the characteristics of hcc patients with liver abscess to improve the differential diagnosis of this condition. from january 2005 to july 2007, the medical records of nine hcc patients (4 females, 5 males; mean age, 65.8 years) trea ... | 2009 | 19767259 |
| in vivo selection of carbapenem-resistant klebsiella pneumoniae by ompk36 loss during meropenem treatment. | we recovered a carbapenem-resistant klebsiella pneumoniae isolate h224 under in vivo meropenem selection pressure. insertional inactivation of a major porin gene, ompk36, by is5 element might play a role in acquiring carbapenem resistance in this strain harboring plasmid-borne dha-1 ampc beta-lactamase. | 2009 | 19766430 |
| description of streptococcus pneumoniae infections in burn patients. | longer survival in burn patients has resulted in more infectious complications, typically with klebsiella pneumoniae, pseudomonas aeruginosa, acinetobacter baumannii, and staphylococcus aureus. although streptococcus pneumoniae infections are common in the community and can cause nosocomial infections, the incidence and risk factors for pneumococcal infections in burn patients is unclear. | 2010 | 19765906 |
| in vitro activities of moxifloxacin and tigecycline against bacterial isolates associated with intraabdominal infections at a medical center in taiwan, 2001-2006. | a total of 569 nonduplicate isolates recovered from patients with community-onset or hospital-onset intraabdominal infections (iais) from 2001 to 2006 were studied. these included 28 staphylococcus aureus and 541 gram-negative isolates (33.6% escherichia coli, 29.0% klebsiella pneumoniae, 8.1% acinetobacter baumannii, and 6.3% pseudomonas aeruginosa). minimum inhibitory concentrations (mics) of the isolates to moxifloxacin, imipenem, and ciprofloxacin were determined using the agar dilution meth ... | 2009 | 19763644 |
| comparison of the bacterial community and characterization of plant growth-promoting rhizobacteria from different genotypes of chrysopogon zizanioides (l.) roberty (vetiver) rhizospheres. | molecular approaches [pcr-denaturing gradient gel electrophoresis (dgge)] were used to determine whether three different vetiver (chrysopogon zizanioides) genotypes, commercially used in brazil and considered economically important over the world, select specific bacterial populations to coexist in their rhizospheres. dgge profiles revealed that the predominant rhizospheric bacterial community hardly varies regarding the vetiver genotype. moreover, using traditional cultivation methods, bacteria ... | 2009 | 19763409 |
| urinary tract infection in children with nephrotic syndrome in kano, nigeria. | nephrotic syndrome is a common childhood renal disorder; the prevalence of urinary tract infection (uti) in these patients is high. the increased prevalence of uti are due to immunoglobulin loss, defective t cell function, presence of ascites and relative malnutrition. | 2009 | 19763005 |
| the optimization of a rapid pulsed-field gel electrophoresis protocol for the typing of acinetobacter baumannii, escherichia coli and klebsiella spp. | pulsed-field gel electrophoresis (pfge) is the most common genotyping method used for the typing of a number of bacterial species. generally, investigators use their own custom-developed protocol, but a standardized pfge protocol would allow the comparison of typing results between laboratories and the tracing of strains around the country. in the present study, we optimized a pfge protocol for subtyping of acinetobacter baumannii, escherichia coli and klebsiella spp., which are commonly isolate ... | 2009 | 19762987 |
| prognostic serum tumor necrosis factor-alpha in paediatric patients with sepsis. | to study the association of tumor necrosis factor-alpha (tnf-alpha) in paediatric patients with different etiological agents and levels of sepsis. | 2009 | 19762956 |
| invasive bacteria isolates from children with severe infections in a nigerian hospital. | little information is available about the aetiology and epidemiology of serious bacterial infections in nigeria. this study determined bacterial isolates from blood and cerebrospinal fluid (csf) of children presenting in the emergency room of a teaching hospital in nigeria. | 2009 | 19762955 |
| enterobacteriaceae and related organisms isolated from nest run cart shelves in commercial shell egg processing facilities. | enterobacteriaceae, including salmonella, may be recovered from foods and processing facilities. high levels of enterobacteriaceae in the processing plant environment can be an indication of inadequate sanitation. this experiment was designed to determine if nest run egg carts serve as reservoirs for enterobacteriaceae. eggs that are produced by hens not housed in buildings connected to the processing plant are referred to as nest run. many of these eggs are transported to the plant on carts to ... | 2009 | 19762864 |
| evaluation of antimicrobial peptides as novel bactericidal agents for room temperature-stored platelets. | a single cost-effective pathogen inactivation approach would help to improve the safety of our nation's blood supply. several methods and technologies are currently being studied to help reduce bacterial contamination of blood components. there is clearly need for simple and easy-to-use pathogen inactivation techniques specific to plasma, platelets (plts), and red blood cells. | 2010 | 19761549 |
| predictors of positive sputum cultures in exacerbations of chronic obstructive pulmonary disease. | although sputum culture in patients with an acute exacerbation of copd is of uncertain value, it is routinely done. the ability to clinically identify patients likely or unlikely to yield bacterial sputum isolates would potentially reduce unnecessary tests. the objective of this study was to identify the clinical predictors of positive sputum cultures in this patient population. | 2009 | 19761538 |
| biofilm growth of individual and dual strains of klebsiella oxytoca from the dairy industry on ultrafiltration membranes. | formation of biofilms in dairy membrane plants causes membrane pore blocking, product contamination and subsequent economic loss. to investigate the biofilm growth, two klebsiella oxytoca strains, k. b006 and tr002, previously isolated from new zealand dairy membrane plants, were grown both individually and combined on three types of ultrafiltration (uf) membranes in different concentrations of whey medium in biofilm reactors (cbr 90, biosurface technologies, bozeman, usa). biofilms of both the ... | 2009 | 19760228 |
| extended spectrum beta-lactamases (esbl) in escherichia coli and klebsiella pneumoniae: trends in the hospital and community settings. | to assess the prevalence of extended spectrum beta-lactamase (esbl) producing escherichia coli and klebsiella strains in nosocomial and community-acquired infections. | 2009 | 19759493 |
| emergence in italy of klebsiella pneumoniae sequence type 258 producing kpc-3 carbapenemase. | 2009 | 19759220 | |
| engineering klebsiella oxytoca for efficient 2, 3-butanediol production through insertional inactivation of acetaldehyde dehydrogenase gene. | ethanol was a major byproduct of 2,3-butanediol (2,3-bd) fermentation by klebsiella oxytoca me-ud-3. in order to achieve a high efficiency of 2,3-bd production, k. oxytoca mutants deficient in ethanol formation were successfully constructed by replace the alda gene coding for aldehyde dehydrogenase with a tetracycline resistance cassette. the results suggested that inactivation of alda led to a significantly improved 2,3-bd production. the carbon flux to 2,3-bd was enhanced by eliminating the by ... | 2010 | 19756578 |
| pharmacological characterization of anaphylaxis-like shock responses induced in mice by mannan and lipopolysaccharide. | intravenous injection of lipopolysaccharide (lps, a component of the gram-negative bacterial cell-surface) or mannan (man, a component of the fungal cell-surface) into mice reportedly induces anaphylaxis-like shock (als) via complement-associated platelet degradation and platelet-activating factor (paf), respectively. however, it is unclear whether paf is involved in lps-als or whether complements and/or platelets are involved in man-als. here, using preparations of man from saccharomyces cerevi ... | 2009 | 19755175 |
| effective strategies for prevention and control of gram-negative infections. | infections caused by gram-negative bacteria such as pseudomonas, klebsiella, proteus and acinetobacter have been a major problem in healthcare settings for many years. overlooked in government targets and unknown to patients, these infections present considerable challenges for effective infection prevention and control. key strategies for prevention and control focus on contact precautions, the management of invasive devices and maintaining a clean, dry environment to prevent the build-up of en ... | 2009 | 19753872 |
| time-dependent effects of klebsiella pneumoniae endotoxin on the pharmacokinetics of chlorzoxazone and its main metabolite, 6-hydroxychlorzoxazone, in rats: restoration of the parameters in 96 hour in kplps rats to control levels. | it has been reported that chlorzoxazone (czx) was primarily metabolized via hepatic cyp2e1 to form 6-hydroxychlorzoxazone (oh-czx) in rats, and the activity of aniline hydroxylase (a cyp2e1 marker) in the liver was significantly decreased in rats at 24 h after pretreatment with lipopolysaccharide derived from klebsiella pneumoniae (24 h kplps rats), whereas the levels were not changed at 2 h and 96 h in the kplps rats. thus, the time-dependent pharmacokinetic parameters of czx and oh-czx were ev ... | 2009 | 19753555 |
| vaccination of melanoma patients with melan-a/mart-1 peptide and klebsiella outer membrane protein p40 as an adjuvant. | toll-like receptor ligands are potentially useful adjuvants for the development of clinical t cell vaccination. here we investigated the novel toll-like receptor2 ligand p40, the outer membrane protein a derived from klebsiella pneumoniae. seventeen human leukocyte antigen-a*0201 positive stage iii/iv melanoma patients were vaccinated with p40 and melan-a/mart-1 peptide subcutaneously in monthly intervals. adverse reactions were mild-to-moderate. fourteen patients received at least 8 vaccination ... | 2009 | 19752746 |
| nonclonal emergence of colistin-resistant klebsiella pneumoniae isolates from blood samples in south korea. | in vitro activities of colistin and other drugs were tested against 221 klebsiella pneumoniae isolates that were collected between 2006 and 2007 in nine tertiary care south korean hospitals from patients with bacteremia. the clonality of colistin-resistant k. pneumoniae (crkp) isolates was assessed by multilocus sequence typing (mlst). we found that 15 isolates (6.8%) were resistant to colistin. mlst showed that crkp isolates were nonclonal, with colistin resistance in k. pneumoniae occurring in ... | 2010 | 19752282 |
| complete nucleotide sequence of pctx-m360, an intermediate plasmid between pel60 and pctx-m3, from a multidrug-resistant klebsiella pneumoniae strain isolated in china. | in this work we report the characterization of plasmid pctx-m360, isolated from a klebsiella pneumoniae strain from china and encoding the ctx-m-3 extended-spectrum beta-lactamase. sequence analysis of pctx-m360 revealed extensive similarity with pel60 and pctx-m3, two other enterobacterial plasmids of the incl/m incompatibility group. compared to pel60, pctx-m360 contains several insertions but lacks most of a 27-kb insert found in pctx-m3, suggesting that it could be an evolutionary intermedia ... | 2009 | 19752275 |
| total knee arthroplasty and crigler-najjar syndrome: a case report. | crigler-najjar (cn) syndrome is a rare genetic disease characterized by hyperbilirubinemia due to a deficiency in the hepatic enzyme udp-glucuronosyl-transferase. we describe the first case of total knee arthroplasty in a patient with cn syndrome (type ii). this procedure was complicated by kernicterus 1 week after hospital discharge. he also developed klebsiella bacteremia and sepsis, requiring a brief icu stay. he was discharged in good condition 2 months later. it is evident that physicians i ... | 2010 | 19751978 |
| in vitro evaluation of the antimicrobial effect of commercially available mastitis medications combined with edta-tris on bacteria that cause mastitis in cattle. | a study was conducted to evaluate the antimicrobial activity of selected commercial mastitis medications with and without edta-tris by comparing minimum inhibitory concentrations and minimum bactericidal concentrations in vitro. solutions containing 5 mm edta/50 mm tris potentiated the antimicrobial action of seven commercially available mastitis medications when reacted in vitro with staphylococcus aureus, streptococcus uberis, and pseudomonas aeruginosa. lesser potentiation was observed for es ... | 2002 | 19750746 |
| prevalence and antimicrobial resistance of health care associated bloodstream infections at a general hospital in saudi arabia. | to determine the trend of health care associated blood stream infection (ha-bsi) at saudi aramco medical services organization. | 2009 | 19750270 |
| plasmid-borne blashv genes in klebsiella pneumoniae are associated with strong promoters. | extended-spectrum beta-lactamases (esbls) belonging to the shv family remain a major cause of esbl-positive phenotypes in klebsiella pneumoniae. the bla(shv) gene is a normal constituent of the k. pneumoniae chromosome. however, most esbl-encoding bla(shv) genes found in k. pneumoniae are plasmid borne. the objective was to determine the contribution of promoter variants to the expression of plasmid-borne bla(shv) genes. | 2009 | 19749204 |
| characterization of class 1 integrons and gene cassettes in clinical isolates of klebsiella pneumoniae from taiwan. | we surveyed the prevalence and contents of class 1 integrons in clinical klebsiella pneumoniae isolates collected from kaohsiung, taiwan, during 2 periods (1993 and 2004). class 1 integrons were present in 78 isolates (34.2%) from 1993 (n = 228) and 129 (32.9%) from 2004 (n = 392) and contained varied gene cassette number, type, and array. we found 2 atypical sul3-associated class 1 integrons and identified 26 different gene cassettes, including an aac(6')-ib-cr cassette here firstly described i ... | 2009 | 19748438 |
| combination of imp-4 metallo-beta-lactamase production and porin deficiency causes carbapenem resistance in a klebsiella oxytoca clinical isolate. | this study shows for the first time the mechanism of carbapenem resistance of a klebsiella oxytoca clinical isolate zc101 recovered from a zhejiang university hospital in hangzhou, china. mic values of imipenem, meropenem, and ertapenem for k. oxytoca zc101 were 16, 16, and 128 microg/ml, respectively. conjugation experiments demonstrated the transferability of a resistance determinant from k. oxytoca zc101 to escherichia coli ec600. results from isoelectric focusing, polymerase chain reactions, ... | 2009 | 19748427 |
| selection of indicator bacteria based on screening of 16s rdna metagenomic library from a two-stage anoxic-oxic bioreactor system degrading azo dyes. | dye degradation has gained attention of late due to indiscriminate disposal from user industries. enhancing efficiency of biological treatment provides a cheaper alternative vis-à-vis other advanced technologies. dye molecules are metabolized biologically via anoxic and oxic treatments. in this study, bacterial community surviving on dye effluent working in anoxic-oxic bioreactor was analyzed using 16s rdna approach. azo-dye decolorizing and degrading bacterial community was enriched in lab-scal ... | 2010 | 19748265 |
| in vitro susceptibilities of aerobic and facultative anaerobic gram-negative bacilli from patients with intra-abdominal infections worldwide from 2005-2007: results from the smart study. | smart (study for monitoring antimicrobial resistance trends) is an ongoing multiyear surveillance study to monitor worldwide antimicrobial resistance trends among aerobic and facultative anaerobic gram-negative bacilli (gnb) isolated from intra-abdominal infections. during 2005-2007, a total of 255 laboratories from 37 countries in five global regions collected intra-abdominal gnb for antimicrobial susceptibility testing using broth microdilution according to clinical and laboratory standards in ... | 2009 | 19748234 |
| prevalence and types of extended-spectrum beta-lactamases among urinary escherichia coli and klebsiella spp. in new zealand. | the aims of this survey were (i) to determine the prevalence of extended-spectrum beta-lactamases (esbls) among urinary escherichia coli and klebsiella spp. in new zealand (nz), (ii) to identify the relative prevalence of esbl types and (iii) to investigate clonality among esbl-producing e. coli and klebsiella spp. during a 4-week period in 2006, 86% of nz hospital and community diagnostic microbiology laboratories participated in the survey and referred isolates to the national reference labora ... | 2009 | 19748232 |
| multidrug-resistant gram-negative infections: what are the treatment options? | the emergence of multidrug-resistant (mdr) gram-negative bacilli creates a challenge in the treatment of nosocomial infections. while the pharmaceutical pipeline is waning, two revived old antibacterials (colistin and fosfomycin), a newer one (tigecycline) and an 'improved' member of an existing class (doripenem) are the only therapeutic options left. the class of polymyxins, known since 1947 and represented mostly by polymyxin b and polymyxin e (colistin), has recently gained a principal role i ... | 2009 | 19747006 |
| pyrroloquinoline quinone biogenesis: demonstration that pqqe from klebsiella pneumoniae is a radical s-adenosyl-l-methionine enzyme. | biogenesis of pyrroloquinoline quinone (pqq) in klebsiella pneumoniae requires the expression of six genes (pqqa-f). one of these genes (pqqe) encodes a 43 kda protein (pqqe) that plays a role in the initial steps in pqq formation [veletrop, j. s., et al. (1995) j. bacteriol. 177, 5088-5098]. pqqe contains two highly conserved cysteine motifs at the n- and c-termini, with the n-terminal motif comprised of a cx(3)cx(2)c consensus sequence that is unique to a family of proteins known as radical s- ... | 2009 | 19746930 |
| antibiotic resistance in pathogens causing community-acquired urinary tract infections in india: a multicenter study. | empiric treatment of community-acquired urinary tract infections (ca-uti) is determined by the antibiotic sensitivity patterns of uropathogens in a population. this study was conducted to determine patterns of resistance amongst ca-uropathogens in india, to help establish local guidelines on treatment of ca-uti. | 2008 | 19745502 |
| development of ertapenem resistance in a patient with mediastinitis caused by klebsiella pneumoniae producing an extended-spectrum beta-lactamase. | the aim was to study the clinical and microbiological features associated with a carbapenem-resistant klebsiella pneumoniae isolate that had been selected in vivo by an ertapenem-containing regimen in a patient with mediastinitis despite high blood and mediastinal levels of ertapenem. carbapenem resistance was characterized by conjugation, pcr, dna sequencing and analysis of outer-membrane proteins. the isolates susceptible and resistant to the carbapenems were compared by ribotyping and pfge. r ... | 2010 | 19745032 |
| genetic diversity of capsular polysaccharide biosynthesis in klebsiella pneumoniae clinical isolates. | klebsiella pneumoniae is an enteric pathogen causing community-acquired and hospital-acquired infections in humans. epidemiological studies have revealed significant diversity in capsular polysaccharide (cps) type and clinical manifestation of k. pneumoniae infection in different geographical areas of the world. we have sequenced the capsular polysaccharide synthesis (cps) region of seven clinical isolates and compared the sequences with the publicly available cps sequence data of five strains: ... | 2009 | 19744990 |
| emergence of blakpc-containing klebsiella pneumoniae in a long-term acute care hospital: a new challenge to our healthcare system. | to characterize isolates of klebsiella pneumoniae producing kpc carbapenemase (kpc-kp) associated with an outbreak in a long-term acute care hospital (ltach) in south florida. | 2009 | 19740911 |
| risk factors for and mortality of extended-spectrum-beta-lactamase-producing klebsiella pneumoniae and escherichia coli nosocomial bloodstream infections. | a case-control study, involving patients with positive blood cultures for klebsiella pneumoniae (kp) or escherichia coli (ec) ec and controls with positive blood cultures for non-esbl-kp or ec, was performed to assess risk factors for extended-spectrum-beta-lactamase (esbl) production from nosocomial bloodstream infections (bsis). mortality among patients with bsis was also assessed. the study included 145 patients (81, 59.5% with k. pneumoniae and 64, 44.1% with e. coli bsi); 51 (35.2%) isolate ... | 2009 | 19739001 |
| extended-spectrum beta-lactamases among enterobacteriaceae isolated in a public hospital in brazil. | extended-spectrum beta-lactamases (esbl) in enterobacteria are recognized worldwide as a great hospital problem. in this study, 127 esbl-producing enterobacteriaceae isolated in one year from inpatients and outpatients at a public teaching hospital at são paulo, brazil, were submitted to analysis by pcr with specific primers for bla shv, bla tem and bla ctx-m genes. from the 127 isolates, 96 (75.6%) klebsiella pneumoniae, 12 (9.3%) escherichia coli, 8 (6.2%) morganella morganii, 3 (2.3%) proteus ... | 2009 | 19739000 |
| first isolation of a vim-producing klebsiella pneumoniae from a seven-year-old child in venezuela. | vim-type metallo-betalactamases (mbls) exhibit hydrolytic activity against most betalactam antibiotics, including carbapenems. so far, vim-type-producing klebsiella pneumoniae isolates had not been reported in latin america. | 2008 | 19738358 |
| influence of inoculum size and marbofloxacin plasma exposure on the amplification of resistant subpopulations of klebsiella pneumoniae in a rat lung infection model. | we tested the hypothesis that the bacterial load at the infection site could impact considerably on the pharmacokinetic/pharmacodynamic (pk/pd) parameters of fluoroquinolones. using a rat lung infection model, we measured the influence of different marbofloxacin dosage regimens on selection of resistant bacteria after infection with a low (10(5) cfu) or a high (10(9) cfu) inoculum of klebsiella pneumoniae. for daily fractionated doses of marbofloxacin, prevention of resistance occurred for an ar ... | 2009 | 19738020 |
| in vitro activity of nemonoxacin, a novel nonfluorinated quinolone, against 2,440 clinical isolates. | the in vitro activity of nemonoxacin (tg-873870), a novel nonfluorinated quinolone, was tested against 2,440 clinical isolates. nemonoxacin was at least fourfold more active than levofloxacin and moxifloxacin against most gram-positive cocci tested (shown by the following mic(90)/range [microg/ml] values; community-associated methicillin [meticillin]-resistant staphylococcus aureus, 0.5/0.015 to 2; staphylococcus epidermidis, 0.5/0.015 to 4 for methicillin-susceptible staphylococci and 2/0.12 to ... | 2009 | 19738018 |
| haemopoietic stem cell transplantation induces tolerance to donor antigens but not to foreign fviii peptides. | a 22-year-old male with severe haemophilia a and high responding factor viii (fviii) inhibitor underwent sibling haematopoietic stem cell transplantation in an attempt to eradicate the inhibitor. a reduced intensity conditioning regimen was followed by bone marrow infusion and continuous fviii administration during immune reconstitution. although substantial levels of fviii:c (>100 iu dl(-1)) were maintained initially, at day +23 inhibitor titres rose, indicating boosting of recipient memory rep ... | 2010 | 19735311 |
| extended spectrum beta-lactamases among gram-negative bacteria from an egyptian pediatric hospital: a two-year experience. | knowledge of the anti-microbial susceptibility pattern of common pathogens in a given area helps to inform the choice of empirical antibiotic therapy. the aim of this study was to determine the existence and to describe the characteristics of extended spectrum beta-lactamase (esbls) in a pediatric hospital in mansoura university, egypt, to aid in the choice of empirical antibiotic therapy. | 2007 | 19734604 |
| comparative structure-function analysis of mannose-specific fimh adhesins from klebsiella pneumoniae and escherichia coli. | fimh, the adhesive subunit of type 1 fimbriae expressed by many enterobacteria, mediates mannose-sensitive binding to target host cells. at the same time, fine receptor-structural specificities of fimh from different species can be substantially different, affecting bacterial tissue tropism and, as a result, the role of the particular fimbriae in pathogenesis. in this study, we compared functional properties of the fimh proteins from escherichia coli and klebsiella pneumoniae, which are both 279 ... | 2009 | 19734306 |
| in vitro activity of tigecycline and comparator agents against a global collection of gram-negative and gram-positive organisms: tigecycline evaluation and surveillance trial 2004 to 2007. | the tigecycline evaluation and surveillance trial began in 2004 to monitor the in vitro activity of tigecycline and comparator agents against a global collection of gram-negative and gram-positive pathogens. against gram negatives (n = 63 699), tigecycline mic(90)'s ranged from 0.25 to 2 mg/l for escherichia coli, haemophilus influenzae, acinetobacter baumannii, klebsiella oxytoca, enterobacter cloacae, klebsiella pneumoniae, and serratia marcescens (but was > or =32 for pseudomonas aeruginosa). ... | 2009 | 19733459 |
| structural and biochemical characterization of the interaction between kpc-2 beta-lactamase and beta-lactamase inhibitor protein. | kpc beta-lactamases hydrolyze the "last resort" beta-lactam antibiotics (carbapenems) used to treat multidrug resistant infections and are compromising efforts to combat life-threatening gram-negative bacterial infections in hospitals worldwide. consequently, the development of novel inhibitors is essential for restoring the effectiveness of existing antibiotics. the beta-lactamase inhibitor protein (blip) is a competitive inhibitor of a number of class a beta-lactamases. in this study, we chara ... | 2009 | 19731932 |
| chemical composition and antimicrobial activity of the essential oil of anaphalis nubigena var. monocephala. | the essential oil composition of the aerial parts of anaphalis nubigena dc. var. monocephala (dc.) c. b. clarke collected from pindari glacier at a height of 3300 m, was analyzed by using gc and gc/ms. sixty components were identified, accounting for 95.9% of the total oil. the main constituents were alpha-guaiene (12.3%), gamma-muurolene (10.4%), gamma-cadinene (8.3%), alpha-muurolol (7.4%), alpha-gurjunene (6.0%) and alpha-bulnesene (5.8%). the oil was found to be rich in sesquiterpene hydroca ... | 2009 | 19731610 |
| international survey of cronobacter sakazakii and other cronobacter spp. in follow up formulas and infant foods. | a coordinated survey for cronobacter and related organisms in powdered infant formula, follow up formula and infant foods was undertaken by 8 laboratories in 7 countries in recognition of and in response to the data needs identified in an fao/who call for data in order to develop global risk management guidance for these products. the products (domestic and imported) were purchased from the local market and were categorised according to their principle ingredients. a total of 290 products were a ... | 2009 | 19729216 |
| characterization of a novel extended-spectrum tem-type beta-lactamase, tem-164, in a clinical strain of klebsiella pneumoniae in tunisia. | klebsiella pneumoniae ml1708 exhibited a multiresistance phenotype, including resistance to all beta-lactams tested, chloramphenicol, ciprofloxacin, nalidixic acid, tetracycline, and streptomycin. the double-disk synergy test was positive. ml1708 harbored a 50 kb conjugative plasmid that encoded a beta-lactamase of pi 5.5. the corresponding bla gene was identified by polymerase chain reaction and sequencing as a bla(tem) gene. the deduced protein sequence revealed a new variant of tem-1 beta-lac ... | 2009 | 19728777 |
| inhibition of frog antimicrobial peptides by extracellular products of the bacterial pathogen aeromonas hydrophila. | to determine whether the extracellular products (ecps) from aeromonas hydrophila, a frog bacterial pathogen that is resistant to skin antimicrobial peptides of three different frog species xenopus laevis, litoria aurea and litoria raniformis, can modulate the activity of these peptides. | 2009 | 19728406 |
| construction of a novel expression system in klebsiella pneumoniae and its application for 1,3-propanediol production. | a novel expression system of klebsiella pneumoniae was developed in order to improve 1,3-propanediol (1,3-pd) production using a k. pneumoniae-escherichia coli shuttle vector pet28a consisting of the kanamycin-resistance gene promoter pkan. the recombinant plasmid petpkan-cat carrying the chloramphenicol acetyltransferase gene cat as selectable marker was constructed to test the availability of the promoter pkan in k. pneumoniae. the results showed that the chloramphenicol acetyltransferase was ... | 2010 | 19728170 |
| incidence of multidrug-resistant organisms causing ventilator-associated pneumonia in a tertiary care hospital: a nine months' prospective study. | ventilator-associated pneumonia (vap) is an important intensive care unit (icu) infection in mechanically ventilated patients. vap occurs approximately in 9-27% of all intubated patients. due to the increasing incidence of multidrug-resistant organisms in icus, early and correct diagnosis of vap is an urgent challenge for an optimal antibiotic treatment. | 2007 | 19727346 |
| pseudomembranous colitis secondary to klebsiella oxytoca. | 2009 | 19727105 | |
| use of boronic acid disk tests to detect extended- spectrum beta-lactamases in clinical isolates of kpc carbapenemase-possessing enterobacteriaceae. | we evaluated boronic acid (ba)-based methods for their ability to detect extended-spectrum beta-lactamases (esbls) among clinical isolates of kpc-producing members of the enterobacteriaceae family. a total of 155 isolates of klebsiella pneumoniae (n = 141), escherichia coli (n = 6), enterobacter aerogenes (n = 6), and klebsiella oxytoca (n = 2) genotypically confirmed to be kpc producers were analyzed. as many as 118 isolates harbored esbls (103 harbored shv-type esbls, 13 harbored ctx-m-type es ... | 2009 | 19726597 |
| comparison of the entericbio multiplex pcr system with routine culture for detection of bacterial enteric pathogens. | the entericbio system uses a multiplex pcr assay for the simultaneous detection of campylobacter spp., salmonella enterica, shigella spp., and escherichia coli o157 from feces. it combines overnight broth enrichment with pcr amplification and detection by hybridization. an evaluation of this system was conducted by comparing the results obtained with the system with those obtained by routine culture, supplemented with alternative pcr detection methods. in a study of 773 samples, routine culture ... | 2009 | 19726596 |
| steady-state pharmacokinetics and pharmacodynamics of piperacillin/tazobactam administered by prolonged infusion in hospitalised patients. | the objective of this study was to evaluate the steady-state pharmacokinetics and pharmacodynamics of piperacillin/tazobactam, administered by prolonged infusion, in hospitalised patients requiring antimicrobial therapy. thirteen patients received 4.5 g every 8 h (q8h), infused over 4 h, and pharmacokinetic parameters were determined by non-compartmental methods. monte carlo simulations (10,000 patients) were performed to calculate the cumulative fraction of response (cfr) for seven gram-negativ ... | 2009 | 19726163 |
| [epidemiology of enterobacteriaceae causing bloodstream infections in neonatal intensive care unit patients]. | an increasing number of neonates require intensive care, and this population has a high risk of developing infections. the enterobacteriaceae most commonly related to healthcare-associated infection in neonatal intensive care units (nicu) are escherichia coli, klebsiella spp and enterobacter cloacae. | 2010 | 19726111 |
| neonatal enteral feeding tubes as loci for colonisation by members of the enterobacteriaceae. | the objective of this study was to determine whether neonatal nasogastric enteral feeding tubes are colonised by the opportunistic pathogen cronobacter spp. (enterobacter sakazakii) and other enterobacteriaceae, and whether their presence was influenced by the feeding regime. | 2009 | 19723318 |
| nosocomial infection rates at an oncology center. | nosocomial infection rates were computed for 5,031 patients at an oncology center during a 20-month period. twelve percent of the patients developed nosocomial infections, accounting for a total of 802 infections. the overall incidence of nosocomial infections during this study period was 6.27 infections per 1,000 patient days. the highest incidence of nosocomial infections was found in patients having acute myelogenous leukemia (30.49 infections per 1,000 patient days); bone and joint cancer (2 ... | 1988 | 19722932 |
| genetic structure and distribution of the colibactin genomic island among members of the family enterobacteriaceae. | a genomic island encoding the biosynthesis and secretion pathway of putative hybrid nonribosomal peptide-polyketide colibactin has been recently described in escherichia coli. colibactin acts as a cyclomodulin and blocks the eukaryotic cell cycle. the origin and prevalence of the colibactin island among enterobacteria are unknown. we therefore screened 1,565 isolates of different genera and species related to the enterobacteriaceae by pcr for the presence of this dna element. the island was dete ... | 2009 | 19720753 |
| biodegradation of the herbicide trifluralin by bacteria isolated from soil. | trifluralin (alpha,alpha,alpha-trifluoro-2,6-dinitro-n,n-dipropyl-p-toluidine; tfl) is a pre-emergence, soil-incorporated herbicide that has been in agricultural use since the early 1960s and is moderately persistent in soil. the purpose of this study was to isolate and characterise tfl-resistant bacteria from a soil in which this pesticide has been used for the last four decades and to determine their ability to degrade tfl using hplc. eight bacteria were isolated by repeated subculture in liqu ... | 2003 | 19719679 |
| x-ray crystallography reveals a reduced substrate complex of udp-galactopyranose mutase poised for covalent catalysis by flavin. | the flavoenzyme uridine 5'-diphosphate galactopyranose mutase (ugm or glf) catalyzes the interconversion of udp-galactopyranose and udp-galactofuranose. the latter is a key building block for cell wall construction in numerous pathogens, including mycobacterium tuberculosis. mechanistic studies of ugm suggested a novel role for the flavin, and we previously provided evidence that the catalytic mechanism proceeds through a covalent flavin-galactose iminium. here, we describe 2.3 and 2.5 a resolut ... | 2009 | 19719175 |
| [influence of bifidobacteria on antilysozyme activity of microorganisms and their ability to form biofilms]. | to assess influence of bifidobacteria's exometabolites on expression of antilysozyme activity of microflora and its ability to biofilm formation. | 2009 | 19718828 |
| [influence of synthetic antioxidants on persistence characteristics of microorganisms]. | to assess antipersistence characteristics of synthetic antioxidants belonging to class of triterpenoids. | 2009 | 19718826 |
| [the autoagglutination factor of plague pathogen--cross-reacting antigen]. | an antigen similar to the autoagglutination factor (af) of plague pathogen in immunochemical specificity was sought in 22 bacterial species. for this, an immunoglobulin anti-af diagnosticum that is the sheep erythrocytes sensitized with rabbit immune serum to the af preparation isolated from the plasmid-free variant of the yersinia pestis strain ev76. the bacteriological study applying a passive hemagglutination assay revealed af-like antigens not only in all study strains (n = 30) of y. pestis, ... | 2009 | 19718822 |
| evaluation of children with urolithiasis. | to describe an evaluation protocol for pediatric stone formers for risk assessment and management strategies. | 2007 | 19718299 |
| plasmid-mediated fluoroquinolone efflux pump gene, qepa, in escherichia coli clinical isolates in korea. | the qepa gene was detected in 4 (0.6%) of 621 nonduplicate escherichia coli clinical isolates collected from blood cultures in korea. three of the 4 qepa-positive isolates contained bla(ctx-m-14) and/or bla(tem-1) genes, but not the e8700 isolate. the qepa gene was successfully transferred and conferred resistance to hydrophilic quinolones, such as norfloxacin and ciprofloxacin, in the recipient. the gene was located in part of is26. two isolates were linked to the truncated rmtb gene. | 2009 | 19717263 |
| bacteriological profile and antimicrobial resistance patterns of clinical bacterial isolates in a university hospital. | the objective of this study was to determine the patterns of bacterial isolates found in blood culture of patients with bactermia in king abdul aziz university hospital in addition to determination of antibiotic resistance. a retrospective analysis of the 672 positive samples collected over the period of december 2006-december 2008. the observed mean age was 40 years with comparable distribution in both genders. 65.2% of the population were non-saudi. 65.5% of isolates were gram positive, mainly ... | 2009 | 19717107 |
| the clinical consequences of antimicrobial resistance. | the continued evolution of antimicrobial resistance in the hospital and more recently in the community threatens to seriously compromise our ability to treat serious infections. the major success of the seven-valent streptococcus pneumoniae vaccine at reducing both infection and resistance has been followed by the emergence of previously minor serotypes that express multiresistance. the almost universal activity of cephalosporins and fluoroquinolones against community escherichia coli strains ha ... | 2009 | 19716760 |
| eradication of an extensive outbreak in a neonatal unit caused by two sequential klebsiella pneumoniae clones harbouring related plasmids encoding an extended-spectrum beta-lactamase. | outbreaks caused by extended-spectrum beta-lactamase (esbl)-producing klebsiella pneumoniae are difficult to control and closure of an affected unit is sometimes necessary. we describe an outbreak of esbl-producing k. pneumoniae in a 28-bed neonatal unit that began in august 2005 and lasted for seven months. weekly rectal swabs were taken from all babies admitted throughout the study period. review of all procedures, contact precautions, thorough environmental cleaning and restriction of cephalo ... | 2009 | 19716201 |
| incidence and clinical relevance of bacterial contamination in preservation solution for liver transplantation. | postoperative infection is considered one of the most important causes of morbidity and mortality after liver transplantation. we prospectively studied the incidence and significance of infections in preservation solutions for liver transplantation. | 2009 | 19715863 |
| [formation of biofilms by fecal strains of enterobacteria and yeast fungi from candida genus]. | to assess the ability of bacteria and candida genus' yeast fungi from human fecal microflora to form biofilms. | 2009 | 19715202 |
| the conjunctival bacterial pattern of diabetics undergoing cataract surgery. | to ascertain the conjunctival bacterial pattern of diabetics undergoing cataract operation to reduce the risk of postoperative endophthalmitis (pe). | 2010 | 19713978 |
| prevalence of ewingella americana in retail fresh cultivated mushrooms (agaricus bisporus, lentinula edodes and pleurotus ostreatus) in zaragoza (spain). | the prevalence and mycopathogenic potential of enterobacteriaceae (especially ewingella americana) in cultivated mushrooms were studied. a total of 95 samples of agaricus bisporus, lentinula edodes and pleurotus ostreatus were analyzed to quantify the enterobacteriaceae and to identify the species isolated. the host pathogenicity test was used to verify their mycopathogenic potential. the genus pseudomonas was also quantified, since it is the predominant bacterial group in cultivated mushrooms. ... | 2004 | 19712317 |
| use of chlorate as a selective inhibitor to distinguish membrane-bound nitrate reductase (nar) and periplasmic nitrate reductase (nap) of dissimilative nitrate reducing bacteria in sediment. | the use of chlorate as a selective inhibitor of dissimilative nitrate reduction was studied using pure cultures of comamonas testosteroni (a denitrifier) and klebsiella pneumoniae (a nitrate-ammonifier) isolated from estuarine sediment, and in sediment slurry. pure culture experiments demonstrated that chlorate selectively inhibited membrane-bound nitrate reductase (nar) activity, probably by blocking nitrate transporters (nark). sediment slurry experiments showed that chlorate inhibited nitrate ... | 2004 | 19712307 |
| attributable mortality rate for carbapenem-resistant klebsiella pneumoniae bacteremia. | to determine the attributable (direct) mortality and morbidity caused by carbapenem-resistant klebsiella pneumoniae bacteremia. | 2009 | 19712030 |
| international clones of klebsiella pneumoniae and escherichia coli with extended-spectrum beta-lactamases in a czech hospital. | a 2-month survey of extended-spectrum beta-lactamase (esbl) producers was performed in a czech hospital. klebsiella pneumoniae produced shv-2, -5, or -12, escherichia coli produced ctx-m-9 or -15, and other species produced tem-92 or -132. all k. pneumoniae and e. coli isolates belonged to sequence types (sts) or clonal complexes (ccs) spread across the world (k. pneumoniae clonal complex 11 [cc11], cc14, and sequence type 101 [st101] and e. coli cc31, cc73, cc131, and cc405) and carried various ... | 2009 | 19710276 |
| pleuritis and suppurative pneumonia associated with a hypermucoviscosity phenotype of klebsiella pneumoniae in california sea lions (zalophus californianus). | the aim of this study is to document the isolation of a hypermucoviscosity (hmv) phenotype of klebsiella pneumoniae from 25 cases of suppurative pneumonia and pleuritis and two cases of abscesses in california sea lions (zalophus californianus) from the central california coast, representing the first report of this zoonotic pathogen from the marine environment and only the second report in non-humans. animals died 2h to 4 days after first being observed sick on beaches. clinical signs varied fr ... | 2010 | 19709820 |
| eric-pcr-based strain-specific detection of phenol-degrading bacteria in activated sludge of wastewater treatment systems. | to evaluate the use of enterobacterial repetitive intergenic consensus pcr (eric-pcr)-derived probes and primers to specifically detect bacterial strains in an activated sludge microbial community. | 2009 | 19708883 |
| seizures associated with levofloxacin: case presentation and literature review. | we present a case of a patient who developed seizures shortly after initiating treatment with levofloxacin and to discuss the potential drug-drug interactions related to the inhibition of cytochrome p450 (cyp) 1a2 in this case, as well as in other cases, of levofloxacin-induced seizures. | 2009 | 19707748 |
| a new approach to study in vivo cellular metabolism using a modellistic analysis of magnetic resonance spectra. | this paper presents a novel approach to study in vivo cellular kinetics by the combined use of magnetic resonance (mr) spectroscopy and odum's system modeling, which includes the entire process of substrate degradation and product formation. the experiment was conducted directly in the mr-probe, where microorganisms (klebsiella planticola) were incubated with high (13)c-glucose and (13)c-xylose concentration. sugar consumptions and (13)c-ethanol production were followed for 17 h and mr-spectra w ... | 2009 | 19706297 |