Publications

TitleAbstractYear
Filter
PMID(sorted descending)
Filter
influence of hydrodynamics on the growth kinetics of glass-adhering pseudomonas putida cells through a parallel plate flow chamber.the objective of this work was to investigate the influence of hydrodynamics on the growth kinetics of surface-adhering pseudomonas putida cells. the results showed in particular that under non substrate-limiting conditions, the early step of bacterial apparent growth rate is lower than those measured with suspended cells. contrary to previously cited authors which explain this behavior to the different adhesive properties of the "daughter"-cells (which makes more probable the detachment of thes ...201324404068
heme enzyme structure and function. 201424400737
structural insights into the specific recognition of n-heterocycle biodenitrogenation-derived substrates by microbial amide hydrolases.n-heterocyclic compounds from industrial wastes, including nicotine, are environmental pollutants or toxicants responsible for a variety of health problems. microbial biodegradation is an attractive strategy for the removal of n-heterocyclic pollutants, during which carbon-nitrogen bonds in n-heterocycles are converted to amide bonds and subsequently severed by amide hydrolases. previous studies have failed to clarify the molecular mechanism through which amide hydrolases selectively recognize d ...201424397579
metabolic engineering of pseudomonas sp. strain vlb120 as platform biocatalyst for the production of isobutyric acid and other secondary metabolites.over the recent years the production of ehrlich pathway derived chemicals was shown in a variety of hosts such as escherichia coli, corynebacterium glutamicum, and yeast. exemplarily the production of isobutyric acid was demonstrated in escherichia coli with remarkable titers and yields. however, these examples suffer from byproduct formation due to the fermentative growth mode of the respective organism. we aim at establishing a new aerobic, chassis for the synthesis of isobutyric acid and othe ...201424397404
diversity of endophytic bacteria in malaysian plants as revealed by 16s rrna encoding gene sequence based method of bacterial identification.bacterial endophytes do have several potential applications in pharmacy, medicine and agricultural biotech industry. the main objective of this study was to understand types of bacterial endophytes associated with dicotyledonous (dicot) and monocotyledonous (monocot) plant species. isolation of the endophytic bacteria was performed using surface-sterilized various tissue samples, and identification of the endophytic bacterial isolates (ebis) was completed using 16s rrna encoding gene sequence si ...201324396249
the complete nucleotide sequence of the carbapenem resistance-conferring conjugative plasmid pld209 from a pseudomonas putida clinical strain reveals a chimeric design formed by modules derived from both environmental and clinical bacteria.the complete sequence of the carbapenem-resistance-conferring conjugative plasmid pld209 from a pseudomonas putida clinical strain is presented. pld209 is formed by 3 well-defined regions: an adaptability module encompassing a tn402-like class 1 integron of clinical origin containing blavim-2 and aaca4 gene cassettes, partitioning and transfer modules, and a replication module derived from plasmids of environmental bacteria. pld209 is thus a mosaic of modules originating in both the clinical and ...201424395220
dechlorination of chloroorganics, decolorization, and simultaneous bioremediation of cr6+ from real tannery effluent employing indigenous bacillus cereus isolate.a native bacillus cereus isolate has been employed, for the first time, for simultaneous decolorization, dechlorination of chloroorganics, and cr(6+) remediation from the real tannery effluent. most of the physicochemical variables in 3:1 diluted effluent were well above the standard prescribed limits, which decreased substantially upon microbial treatment. the extent of bioremediation was better in diluted (3:1) as compared to undiluted effluent supplemented with nutrients and augmented with b. ...201424390198
distribution, diversity, and activities of sulfur dioxygenases in heterotrophic bacteria.sulfur oxidation by chemolithotrophic bacteria is well known; however, sulfur oxidation by heterotrophic bacteria is often ignored. sulfur dioxygenases (sdos) (ec 1.13.11.18) were originally found in the cell extracts of some chemolithotrophic bacteria as glutathione (gsh)-dependent sulfur dioxygenases. gsh spontaneously reacts with elemental sulfur to generate glutathione persulfide (gssh), and sdos oxidize gssh to sulfite and gsh. however, sdos have not been characterized for bacteria, includi ...201424389926
nad(p)h-independent asymmetric c=c bond reduction catalyzed by ene reductases by using artificial co-substrates as the hydrogen donor.to develop a nicotinamide-independent single flavoenzyme system for the asymmetric bioreduction of c=c bonds, four types of hydrogen donor, encompassing more than 50 candidates, were investigated. six highly potent, cheap, and commercially available co-substrates were identified that (under the optimized conditions) resulted in conversions and enantioselectivities comparable with, or even superior to, those obtained with traditional two-enzyme nicotinamide adenine dinucleotide phosphate (nad(p)h ...201324382795
nad(p)h-independent asymmetric c=c bond reduction catalyzed by ene reductases by using artificial co-substrates as the hydrogen donor.to develop a nicotinamide-independent single flavoenzyme system for the asymmetric bioreduction of c=c bonds, four types of hydrogen donor, encompassing more than 50 candidates, were investigated. six highly potent, cheap, and commercially available co-substrates were identified that (under the optimized conditions) resulted in conversions and enantioselectivities comparable with, or even superior to, those obtained with traditional two-enzyme nicotinamide adenine dinucleotide phosphate (nad(p)h ...201324382795
methods for facilitating microbial growth on pulp mill waste streams and characterization of the biodegradation potential of cultured microbes.the kraft process is applied to wood chips for separation of lignin from the polysaccharides within lignocellulose for pulp that will produce a high quality paper. black liquor is a pulping waste generated by the kraft process that has potential for downstream bioconversion. however, the recalcitrant nature of the lignocellulose resources, its chemical derivatives that constitute the majority of available organic carbon within black liquor, and its basic ph present challenges to microbial biodeg ...201324378616
[isolation, identification and characterization of five pseudomonas strains].we isolated pseudomonas strains from soil samples collected from changsha, hunan province, catalogued them and studied the antimicrobial and antitumor activity.201324377248
genes involved in degradation of para-nitrophenol are differentially arranged in form of non-contiguous gene clusters in burkholderia sp. strain sj98.biodegradation of para-nitrophenol (pnp) proceeds via two distinct pathways, having 1,2,3-benzenetriol (bt) and hydroquinone (hq) as their respective terminal aromatic intermediates. genes involved in these pathways have already been studied in different pnp degrading bacteria. burkholderia sp. strain sj98 degrades pnp via both the pathways. earlier, we have sequenced and analyzed a ~41 kb fragment from the genomic library of strain sj98. this dna fragment was found to harbor all the lower pathw ...201324376843
alkane hydroxylase gene (alkb) phylotype composition and diversity in northern gulf of mexico bacterioplankton.natural and anthropogenic activities introduce alkanes into marine systems where they are degraded by alkane hydroxylases expressed by phylogenetically diverse bacteria. partial sequences for alkb, one of the structural genes of alkane hydroxylase, have been used to assess the composition of alkane-degrading communities, and to determine their responses to hydrocarbon inputs. we present here the first spatially extensive analysis of alkb in bacterioplankton of the northern gulf of mexico (ngom), ...201324376439
subtilase sprp exerts pleiotropic effects in pseudomonas aeruginosa.the open reading frame pa1242 in the genome of pseudomonas aeruginosa pao1 encodes a putative protease belonging to the peptidase s8 family of subtilases. the respective enzyme termed sprp consists of an n-terminal signal peptide and a so-called s8 domain linked by a domain of unknown function (duf). presumably, this duf domain defines a discrete class of pseudomonas proteins as homologous domains can be identified almost exclusively in proteins of the genus pseudomonas. the sprp gene was expres ...201324376018
subtilase sprp exerts pleiotropic effects in pseudomonas aeruginosa.the open reading frame pa1242 in the genome of pseudomonas aeruginosa pao1 encodes a putative protease belonging to the peptidase s8 family of subtilases. the respective enzyme termed sprp consists of an n-terminal signal peptide and a so-called s8 domain linked by a domain of unknown function (duf). presumably, this duf domain defines a discrete class of pseudomonas proteins as homologous domains can be identified almost exclusively in proteins of the genus pseudomonas. the sprp gene was expres ...201324376018
production of lycopene in the non-carotenoid-producing yeast yarrowia lipolytica.the codon-optimized genes crtb and crti of pantoea ananatis were expressed in yarrowia lipolytica under the control of the tef1 promoter of y. lipolytica. additionally, the rate-limiting genes for isoprenoid biosynthesis in y. lipolytica, ggs1 and hmg1, were overexpressed to increase the production of lycopene. all of the genes were also expressed in a y. lipolytica strain with pox1 to pox6 and gut2 deleted, which led to an increase in the size of lipid bodies and a further increase in lycopene ...201424375130
interspecies signalling: pseudomonas putida efflux pump ttgghi is activated by indole to increase antibiotic resistance.in gram-negative bacteria, multidrug efflux pumps are responsible for the extrusion of chemicals that are deleterious for growth. some of these efflux pumps are induced by endogenously produced effectors, while abiotic or biotic signals induce the expression of other efflux pumps. in pseudomonas putida, the ttgabc efflux pump is the main antibiotic extrusion system that respond to exogenous antibiotics through the modulation of the expression of this operon mediated by ttgr. the plasmid-encoded ...201424373097
the prc and rsep proteases control bacterial cell-surface signalling activity.extracytoplasmic function (ecf) sigma factors play a key role in the regulation of vital functions in the bacterial response to the environment. in gram-negative bacteria, activity of these sigma factors is often controlled by cell-surface signalling (css), a regulatory system that also involves an outer membrane receptor and a transmembrane anti-sigma factor. to get more insight into the molecular mechanism behind css regulation, we have focused on the unique iut system of pseudomonas putida. t ...201424373018
draft genome sequence of pseudomonas putida strain s610, a seed-borne bacterium of wheat.we report the genome sequence of a seed-borne bacterium, pseudomonas putida strain s610. the size of the draft genome sequence is approximately 4.6 mb, which is the smallest among all p. putida strains sequenced to date.201324371199
coffee husk composting: an investigation of the process using molecular and non-molecular tools.various parameters were measured during a 90-day composting process of coffee husk with cow dung (pile 1), with fruit/vegetable wastes (pile 2) and coffee husk alone (pile 3). samples were collected on days 0, 32 and 90 for chemical and microbiological analyses. c/n ratios of piles 1 and 2 decreased significantly over the 90 days. the highest bacterial counts at the start of the process and highest actinobacterial counts at the end of the process (piles 1 and 2) indicated microbial succession wi ...201424369846
use of snares for the immobilization of poly-3-hydroxyalkanoate polymerase type ii of pseudomonas putida ca-3 in secretory vesicles of saccharomyces cerevisiae atcc 9763.polyhydroxyalkanoate (pha) synthase, the key enzyme in polyester biosynthesis of bacteria, has been targeted to various organelles in yeasts and plants using respective signal peptides. here, we report that the sequences derived from snare domains efficiently target and integrate the pha synthase from pseudomonas putida ca-3 to the membrane of secretory vesicles in saccharomyces cerevisiae. the studies with the enhanced green fluorescent protein confirm the localization of synthase enzyme in the ...201424368219
manganese acquisition and homeostasis at the host-pathogen interface.pathogenic bacteria acquire transition metals for cell viability and persistence of infection in competition with host nutritional defenses. the human host employs a variety of mechanisms to stress the invading pathogen with both cytotoxic metal ions and oxidative and nitrosative insults while withholding essential transition metals from the bacterium. for example, the s100 family protein calprotectin (cp) found in neutrophils is a calcium-activated chelator of extracellular mn and zn and is fou ...201324367765
antioxidant, antimicrobial activity and total phenolic content within the aerial parts of artemisia absinthum, artemisia santonicum and saponaria officinalis.three native turkish medicinal and aromatic plants (artemisia absinthum, artemisia santonicum and saponaria officinalis) were investigated to analyze their antioxidant activity, total phenolic content and antimicrobial activity. their total antioxidant activity was determined by using a β-carotene bleaching assay and their antimicrobial activity was determined by utilizing an agar disc diffusion assay. methanol extracts of the three species analyzed showed high antioxidant activity and among the ...201124363680
transcriptomics-based screen for genes induced by flagellin and repressed by pathogen effectors identifies a cell wall-associated kinase involved in plant immunity.microbe-associated molecular patterns, such as those present in bacterial flagellin, are powerful inducers of the innate immune response in plants. successful pathogens deliver virulence proteins, termed effectors, into the plant cell where they can interfere with the immune response and promote disease. engineering the plant immune system to enhance disease resistance requires a thorough understanding of its components.201324359686
biochemical and structural analysis of rraa proteins to decipher their relationships with 4-hydroxy-4-methyl-2-oxoglutarate/4-carboxy-4-hydroxy-2-oxoadipate aldolases.4-hydroxy-4-methyl-2-oxoglutarate (hmg)/4-carboxy-4-hydroxy-2-oxoadipate (cha) aldolases are class ii (divalent metal ion dependent) pyruvate aldolases from the meta cleavage pathways of protocatechuate and gallate. the enzyme from pseudomonas putida f1 is structurally similar to a group of proteins termed regulators of rnase e activity a (rraa) that bind to the regulatory domain of rnase e and inhibit the ribonuclease activity in certain bacteria. analysis of homologous rraa-like proteins from ...201424359411
distribution of endophytic bacteria in alopecurus aequalis sobol and oxalis corniculata l. from soils contaminated by polycyclic aromatic hydrocarbons.the distributions of endophytic bacteria in alopecurus aequalis sobol and oxalis corniculata l. grown in soils contaminated with different levels of polycyclic aromatic hydrocarbons (pahs) were investigated with polymerase chain reaction followed by denaturing gradient gel electrophoresis technology (pcr-dgge) and cultivation methods. twelve types of pahs, at concentrations varying from 0.16 to 180 mg·kg(-1), were observed in the roots and shoots of the two plants. the total pah concentrations i ...201324358247
biological nitrate removal processes from drinking water supply-a review.this paper reviews both heterotrophic and autotrophic processes for the removal of nitrate from water supplies. the most commonly used carbon sources in heterotrophic denitrification are methanol, ethanol and acetic acid. process performance for each feed stock is compared with particular reference nitrate and nitrite residual and to toxicity potential. autotrophic nitrate removal has the advantages of not requiring an organic carbon source; however the slow growth rate of autotrophic bacteria a ...201324355262
microbial alkyl- and aryl-sulfatases: mechanism, occurrence, screening and stereoselectivities.this review gives an overview on the occurrence of sulfatases in prokaryota, eukaryota and archaea. the mechanism of enzymes acting with retention or inversion of configuration during sulfate ester hydrolysis is discussed taking two complementary examples. methods for the discovery of novel alkyl sulfatases are described by way of sequence-based search and enzyme induction. a comprehensive list of organisms with their respective substrate scope regarding prim- and sec-alkyl sulfate esters allows ...201324352732
microbial alkyl- and aryl-sulfatases: mechanism, occurrence, screening and stereoselectivities.this review gives an overview on the occurrence of sulfatases in prokaryota, eukaryota and archaea. the mechanism of enzymes acting with retention or inversion of configuration during sulfate ester hydrolysis is discussed taking two complementary examples. methods for the discovery of novel alkyl sulfatases are described by way of sequence-based search and enzyme induction. a comprehensive list of organisms with their respective substrate scope regarding prim- and sec-alkyl sulfate esters allows ...201324352732
intrigues and intricacies of the biosynthetic pathways for the enzymatic quinocofactors: pqq, ttq, ctq, tpq, and ltq. 201324350630
intrigues and intricacies of the biosynthetic pathways for the enzymatic quinocofactors: pqq, ttq, ctq, tpq, and ltq. 201324350630
practical considerations and challenges involved in surfactant enhanced bioremediation of oil.surfactant enhanced bioremediation (seb) of oil is an approach adopted to overcome the bioavailability constraints encountered in biotransformation of nonaqueous phase liquid (napl) pollutants. fuel oils contain n-alkanes and other aliphatic hydrocarbons, monoaromatics, and polynuclear aromatic hydrocarbons (pahs). although hydrocarbon degrading cultures are abundant in nature, complete biodegradation of oil is rarely achieved even under favorable environmental conditions due to the structural c ...201324350261
biochemical, transcriptional and translational evidences of the phenol-meta-degradation pathway by the hyperthermophilic sulfolobus solfataricus 98/2.phenol is a widespread pollutant and a model molecule to study the biodegradation of monoaromatic compounds. after a first oxidation step leading to catechol in mesophilic and thermophilic microorganisms, two main routes have been identified depending on the cleavage of the aromatic ring: ortho involving a catechol 1,2 dioxygenase (c12d) and meta involving a catechol 2,3 dioxygenase (c23d). our work aimed at elucidating the phenol-degradation pathway in the hyperthermophilic archaea sulfolobus s ...201324349276
role of tomato lipoxygenase d in wound-induced jasmonate biosynthesis and plant immunity to insect herbivores.in response to insect attack and mechanical wounding, plants activate the expression of genes involved in various defense-related processes. a fascinating feature of these inducible defenses is their occurrence both locally at the wounding site and systemically in undamaged leaves throughout the plant. wound-inducible proteinase inhibitors (pis) in tomato (solanum lycopersicum) provide an attractive model to understand the signal transduction events leading from localized injury to the systemic ...201324348260
the hbm domain: introducing bimodularity to bacterial sensing.we have recently reported the three dimensional structure of the mcps chemoreceptor sensor domain in complex with its cognate ligands. the domain was characterized by a bimodular architecture, where ligand binding to each module caused a chemotactic response. this is a novel small molecule binding domain, which, however, is un-annotated in relevant databases. we report here the domain signature of the family of mcps-like sensor domains, which was termed helical bimodular (hbm) domain. the hbm do ...201424347303
the hbm domain: introducing bimodularity to bacterial sensing.we have recently reported the three dimensional structure of the mcps chemoreceptor sensor domain in complex with its cognate ligands. the domain was characterized by a bimodular architecture, where ligand binding to each module caused a chemotactic response. this is a novel small molecule binding domain, which, however, is un-annotated in relevant databases. we report here the domain signature of the family of mcps-like sensor domains, which was termed helical bimodular (hbm) domain. the hbm do ...201424347303
synthesis, characterization, cytotoxicity and antibacterial activity of an anthracenyl-linked bis(pyrazolyl)methane ligand and its zinc(ii) complexes.three novel zn(ii) complexes (1-3) with 1,1'-(anthracen-9-ylmethylene)bis(1h-pyrazole) (l(2)) have been prepared and structurally characterized by x-ray crystallography. among them, 1 is a binuclear zn(ii) complex while 2 and 3 are mononuclear. the in vitro cytotoxic and antibacterial activities of these complexes were also evaluated. the three complexes exhibit cytotoxic specificity and significant antitumor activity. the mic50 value of complex 1 against pseudomonas putida reaching 0.011 μg/ml ...201424342667
comprehensive analysis of the corynebacterium glutamicum transcriptome using an improved rnaseq technique.the use of rnaseq to resolve the transcriptional organization of an organism was established in recent years and also showed the complexity and dynamics of bacterial transcriptomes. the aim of this study was to comprehensively investigate the transcriptome of the industrially relevant amino acid producer and model organism corynebacterium glutamicum by rnaseq in order to improve its genome annotation and to describe important features for transcription and translation.201324341750
a second chromosomal copy of the cata gene endows pseudomonas putida mt-2 with an enzymatic safety valve for excess of catechol.pseudomonas putida mt-2 harbours two different routes for catabolism of catechol, namely one meta pathway encoded by the xyl genes of the tol plasmid pww0 and one ortho pathway determined by the chromosomal ben and cat genes. p. putida mt-2 has a second chromosomal copy of the cata gene (named cata2) located downstream of the ben operon that encodes an additional catechol-1,2-dioxygenase. the metabolic and regulatory phenotypes of strains lacking one enzyme, the other and both of them in cells w ...201424341396
the private life of environmental bacteria: pollutant biodegradation at the single cell level.bacteria display considerable cell-to-cell heterogeneity in a number of genetic and physiological traits. stochastic differences in regulatory patterns (e.g. at the transcriptional level) propagate into the metabolic and physiological status of otherwise isogenic cells, which ultimately results in appearance of sub-populations within the community. as new technologies emerge and because novel single cell strategies are constantly being refined, our knowledge on microbial individuality is in burg ...201424341371
[anti-diatom compounds from marine bacterium pseudomonas putida].in order to provide more natural antifouling compounds, marine bacterium pseudomonas putida isolated from the sponge haliclona sp. was explored to test its anti-diatom compounds.201324341274
biotoxin detection using cell-based sensors.cell-based biosensors (cbbs) utilize the principles of cell-based assays (cbas) by employing living cells for detection of different analytes from environment, food, clinical, or other sources. for toxin detection, cbbs are emerging as unique alternatives to other analytical methods. the main advantage of using cbbs for probing biotoxins and toxic agents is that cbbs respond to the toxic exposures in the manner related to actual physiologic responses of the vulnerable subjects. the results obtai ...201324335754
alcoholytic cleavage of polyhydroxyalkanoate chains by class iv synthases induced by endogenous and exogenous ethanol.polyhydroxyalkanoate (pha)-producing bacillus strains express class iv pha synthase, which is composed of the subunits phar and phac. recombinant escherichia coli expressing pha synthase from bacillus cereus strain yb-4 (pharcyb-4) showed an unusual reduction of the molecular weight of pha produced during the stationary phase of growth. nuclear magnetic resonance analysis of the low-molecular-weight pha revealed that its carboxy end structure was capped by ethanol, suggesting that the molecular ...201424334666
the presence of conjugative plasmid pls20 affects global transcription of its bacillus subtilis host and confers beneficial stress resistance to cells.conjugation activity of plasmid pls20 from bacillus subtilis subsp. natto is induced when cells are diluted into fresh medium and diminishes as cells enter into stationary-phase growth. transcriptional profiling shows that during mid-exponential growth, more than 5% of the host genes are affected in the presence of the plasmid, in contrast to the minor changes seen in freshly diluted and stationary-phase cells. changes occurred in many metabolic pathways, although pls20 does not confer any detec ...201424334659
characteristics of urinary tract infection pathogens and their in vitro susceptibility to antimicrobial agents in china: data from a multicenter study.this study assessed the characteristics of pathogens identified in clinical isolates from patients with urinary tract infection (uti) and their in vitro sensitivity to commonly used antibiotics in the clinical setting in china.201324334199
new insights in the formation of polyhydroxyalkanoate granules (carbonosomes) and novel functions of poly(3-hydroxybutyrate).the metabolism of polyhydroxybutyrate (phb) and related polyhydroxyalkanoates (phas) has been investigated by many groups for about three decades, and good progress was obtained in understanding the mechanisms of biosynthesis and biodegradation of this class of storage molecules. however, the molecular events that happen at the onset of phb synthesis and the details of the initiation of phb/pha granule formation, as well as the complex composition of the proteinaceous surface layer of phb/pha gr ...201424329995
aerobic granulation of aggregating consortium x9 isolated from aerobic granules and role of cyclic di-gmp.this study monitored the granulation process of an aggregating functional consortium x9 that was consisted of pseudomonas putida x-1, acinetobacter sp. x-2, alcaligenes sp. x-3 and comamonas testosteroni x-4 in shaken reactors. the growth curve of x9 was fit using logistic model as follows y=1.49/(1+21.3*exp(-0.33x)), the maximum specific cell growth rate for x9 was 0.33 h(-1). initially x9 consumed polysaccharides (ps) and secreted proteins (pn) to trigger granulation. then x9 grew in biomass a ...201424326212
combination of degradation pathways for naphthalene utilization in rhodococcus sp. strain tfb.rhodococcus sp. strain tfb is a metabolic versatile bacterium able to grow on naphthalene as the only carbon and energy source. applying proteomic, genetic and biochemical approaches, we propose in this paper that, at least, three coordinated but independently regulated set of genes are combined to degrade naphthalene in tfb. first, proteins involved in tetralin degradation are also induced by naphthalene and may carry out its conversion to salicylaldehyde. this is the only part of the naphthale ...201324325207
combination of degradation pathways for naphthalene utilization in rhodococcus sp. strain tfb.rhodococcus sp. strain tfb is a metabolic versatile bacterium able to grow on naphthalene as the only carbon and energy source. applying proteomic, genetic and biochemical approaches, we propose in this paper that, at least, three coordinated but independently regulated set of genes are combined to degrade naphthalene in tfb. first, proteins involved in tetralin degradation are also induced by naphthalene and may carry out its conversion to salicylaldehyde. this is the only part of the naphthale ...201324325207
pseudomonas aeruginosa enhances production of a non-alginate exopolysaccharide during long-term colonization of the cystic fibrosis lung.the gram-negative opportunistic pathogen pseudomonas aeruginosa is the primary cause of chronic respiratory infections in individuals with the heritable disease cystic fibrosis (cf). these infections can last for decades, during which time p. aeruginosa has been proposed to acquire beneficial traits via adaptive evolution. because cf lacks an animal model that can acquire chronic p. aeruginosa infections, identifying genes important for long-term in vivo fitness remains difficult. however, since ...201324324811
construction and optimization of a pseudomonas putida whole-cell bioreporter for detection of bioavailable copper.a pseudomonas putida whole-cell bioreporter for detecting bioavailable copper was constructed by inserting a cuer-regulated sensor element upstream of a promoterless green fluorescent protein (gfp) reporter gene. the constructed bioreporter cells expressed gfp only in response to cu and ag when cultivated in different metal salt solutions. m9 supplemented medium provided higher sensitivity compared with lb medium. the optimal test condition was cell suspension with an od600 of 0.4-0.5 incubated ...201424322773
identification of 3-sulfinopropionyl coenzyme a (coa) desulfinases within the acyl-coa dehydrogenase superfamily.in a previous study, the essential role of 3-sulfinopropionyl coenzyme a (3sp-coa) desulfinase acyl-coa dehydrogenase (acd) in advenella mimigardefordensis strain dpn7(t) (acddpn7) during degradation of 3,3'-dithiodipropionic acid (dtdp) was elucidated. dtdp is a sulfur-containing precursor substrate for biosynthesis of polythioesters (ptes). acddpn7 showed high amino acid sequence similarity to acyl-coa dehydrogenases but was unable to catalyze a dehydrogenation reaction. hence, it was investig ...201424317404
metagenomics and novel gene discovery: promise and potential for novel therapeutics.metagenomics provides a means of assessing the total genetic pool of all the microbes in a particular environment, in a culture-independent manner. it has revealed unprecedented diversity in microbial community composition, which is further reflected in the encoded functional diversity of the genomes, a large proportion of which consists of novel genes. herein, we review both sequence-based and functional metagenomic methods to uncover novel genes and outline some of the associated problems of e ...201324317337
metagenomics and novel gene discovery: promise and potential for novel therapeutics.metagenomics provides a means of assessing the total genetic pool of all the microbes in a particular environment, in a culture-independent manner. it has revealed unprecedented diversity in microbial community composition, which is further reflected in the encoded functional diversity of the genomes, a large proportion of which consists of novel genes. herein, we review both sequence-based and functional metagenomic methods to uncover novel genes and outline some of the associated problems of e ...201324317337
mycobacterium tuberculosis rv2179c protein establishes a new exoribonuclease family with broad phylogenetic distribution.ribonucleases (rnases) maintain the cellular rna pool by rna processing and degradation. in many bacteria, including the human pathogen mycobacterium tuberculosis (mtb), the enzymes mediating several central rna processing functions are still unknown. here, we identify the hypothetical mtb protein rv2179c as a highly divergent exoribonuclease. although the primary sequence of rv2179c has no detectable similarity to any known rnase, the rv2179c crystal structure reveals an rnase fold. active site ...201324311791
mycobacterium tuberculosis rv2179c protein establishes a new exoribonuclease family with broad phylogenetic distribution.ribonucleases (rnases) maintain the cellular rna pool by rna processing and degradation. in many bacteria, including the human pathogen mycobacterium tuberculosis (mtb), the enzymes mediating several central rna processing functions are still unknown. here, we identify the hypothetical mtb protein rv2179c as a highly divergent exoribonuclease. although the primary sequence of rv2179c has no detectable similarity to any known rnase, the rv2179c crystal structure reveals an rnase fold. active site ...201324311791
diversity of epothilone producers among sorangium strains in producer-positive soil habitats.large-scale surveys show that the anti-tumour compounds known as epothilones are produced by only a small proportion of sorangium strains, thereby greatly hampering the research and development of these valuable compounds. in this study, to investigate the niche diversity of epothilone-producing sorangium strains, we re-surveyed four soil samples where epothilone producers were previously found. compared with the < 2.5% positive strains collected from different places, epothilone producers compr ...201424308800
diversity of epothilone producers among sorangium strains in producer-positive soil habitats.large-scale surveys show that the anti-tumour compounds known as epothilones are produced by only a small proportion of sorangium strains, thereby greatly hampering the research and development of these valuable compounds. in this study, to investigate the niche diversity of epothilone-producing sorangium strains, we re-surveyed four soil samples where epothilone producers were previously found. compared with the < 2.5% positive strains collected from different places, epothilone producers compr ...201424308800
nrsz: a novel, processed, nitrogen-dependent, small non-coding rna that regulates pseudomonas aeruginosa pao1 virulence.the opportunistic pathogen pseudomonas aeruginosa pao1 has a remarkable capacity to adapt to various environments and to survive with limited nutrients. here, we report the discovery and characterization of a novel small non-coding rna: nrsz (nitrogen-regulated srna). we show that under nitrogen limitation, nrsz is induced by the ntrb/c two component system, an important regulator of nitrogen assimilation and p. aeruginosa's swarming motility, in concert with the alternative sigma factor rpon. f ...201324308329
nrsz: a novel, processed, nitrogen-dependent, small non-coding rna that regulates pseudomonas aeruginosa pao1 virulence.the opportunistic pathogen pseudomonas aeruginosa pao1 has a remarkable capacity to adapt to various environments and to survive with limited nutrients. here, we report the discovery and characterization of a novel small non-coding rna: nrsz (nitrogen-regulated srna). we show that under nitrogen limitation, nrsz is induced by the ntrb/c two component system, an important regulator of nitrogen assimilation and p. aeruginosa's swarming motility, in concert with the alternative sigma factor rpon. f ...201324308329
back propagation neural network model for predicting the performance of immobilized cell biofilters handling gas-phase hydrogen sulphide and ammonia.lab scale studies were conducted to evaluate the performance of two simultaneously operated immobilized cell biofilters (icbs) for removing hydrogen sulphide (h2s) and ammonia (nh3) from gas phase. the removal efficiencies (res) of the biofilter treating h2s varied from 50 to 100% at inlet loading rates (ilrs) varying up to 13 g h2s/m(3) ·h, while the nh3 biofilter showed res ranging from 60 to 100% at ilrs varying between 0.5 and 5.5 g nh3/m(3) ·h. an application of the back propagation neural ...201324307999
swimming, swarming, twitching, and chemotactic responses of cupriavidus metallidurans ch34 and pseudomonas putida mt2 in the presence of cadmium.to use of microorganisms for bioremediation purposes, the study of their motility behavior toward metals is essential. in the present study, cupriavidus metallidurans ch34 and pseudomonas putida mt2 were used as cadmium (cd)-resistant and -sensitive bacteria, respectively, to evaluate the effects of cd on their motility behaviors. potassium morpholinopropane sulfonate (mops) buffer was used to observe the motility behavior of both isolates. movement of mt2 was less in mops buffer compared with c ...201424306627
kinetic parameters and cytotoxic activity of recombinant methionine γ-lyase from clostridium tetani, clostridium sporogenes, porphyromonas gingivalis and citrobacter freundii.the steady-state kinetic parameters of pyridoxal 5'-phosphate-dependent recombinant methionine γ -lyase from three pathogenic bacteria, clostridium tetani, clostridium sporogenes, and porphyromonas gingivalis, were determined in β- and γ-elimination reactions. the enzyme from c. sporogenes is characterized by the highest catalytic efficiency in the γ-elimination reaction of l-methionine. it was demonstrated that the enzyme from these three sources exists as a tetramer. the n-terminal poly-histid ...201324303205
evaluation of a fret-peptide substrate to predict virulence in pseudomonas aeruginosa.pseudomonas aeruginosa produces a number of proteases that are associated with virulence and disease progression. a substrate able to detect p. aeruginosa-specific proteolytic activity could help to rapidly alert clinicians to the virulence potential of individual p. aeruginosa strains. for this purpose we designed a set of p. aeruginosa-specific fluorogenic substrates, comprising fluorescence resonance energy transfer (fret)-labeled peptides, and evaluated their applicability to p. aeruginosa v ...201324303047
nitrogen assimilation in escherichia coli: putting molecular data into a systems perspective.we present a comprehensive overview of the hierarchical network of intracellular processes revolving around central nitrogen metabolism in escherichia coli. the hierarchy intertwines transport, metabolism, signaling leading to posttranslational modification, and transcription. the protein components of the network include an ammonium transporter (amtb), a glutamine transporter (glnhpq), two ammonium assimilation pathways (glutamine synthetase [gs]-glutamate synthase [glutamine 2-oxoglutarate ami ...201324296575
ingestibility, digestibility, and engineered biological control potential of flavobacterium hibernum, isolated from larval mosquito habitats.flavobacterium hibernum, isolated from larval habitats of the eastern tree hole mosquito, a. triseriatus, remained suspended in the larval feeding zone much longer (8 days) than other bacteria. autofluorescent protein markers were developed for the labeling of f. hibernum with a strong flavobacterial expression system. green fluorescent protein (gfp)-tagged f. hibernum cells were quickly consumed by larval mosquitoes at an ingestion rate of 9.5 × 10(4)/larva/h. the ingested f. hibernum cells wer ...201424296502
transcriptional profiling of genes involved in n-hexadecane compounds assimilation in the hydrocarbon degrading dietzia cinnamea p4 strain.the petroleum-derived degrading dietzia cinnamea strain p4 recently had its genome sequenced and annotated. this allowed employing the data on genes that are involved in the degradation of n-alkanes. to examine the physiological behavior of strain p4 in the presence of n-alkanes, the strain was grown under varying conditions of ph and temperature. d. cinnamea p4 was able to grow at ph 7.0-9.0 and at temperatures ranging from 35 ºc to 45 ºc. experiments of gene expression by real-time quantitativ ...201324294263
in situ biosurfactant production and hydrocarbon removal by pseudomonas putida cb-100 in bioaugmented and biostimulated oil-contaminated soil.in situ biosurfactant (rhamnolipid) production by pseudomonas putida cb-100 was achieved during a bioaugmented and biostimulated treatment to remove hydrocarbons from aged contaminated soil from oil well drilling operations. rhamnolipid production and contaminant removal were determined for several treatments of irradiated and non-irradiated soils: nutrient addition (nitrogen and phosphorus), p. putida addition, and addition of both (p. putida and nutrients). the results were compared against a ...201324294259
the influence of bioaugmentation and biosurfactant addition on bioremediation efficiency of diesel-oil contaminated soil: feasibility during field studies.the study focused on assessing the influence of bioaugmentation and addition of rhamnolipids on diesel oil biodegradation efficiency during field studies. initial laboratory studies (measurement of emitted co2 and dehydrogenase activity) were carried out in order to select the consortium for bioaugmentation as well as to evaluate the most appropriate concentration of rhamnolipids. the selected consortium consisted of following bacterial taxa: aeromonas hydrophila, alcaligenes xylosoxidans, gordo ...201424291585
toxicity assessment of sic nanofibers and nanorods against bacteria.in the present study, evidence of the antibacterial effects of silicon carbide (sic) nanofibers (nfsic) and nanorods (nrsic) obtained by combustion synthesis has been presented. it has been shown that the examined bacteria, pseudomonas putida, could bind to the surface of the investigated sic nanostructures. the results of respiration measurements, dehydrogenase activity measurements, and evaluation of viable bacteria after incubation with nfsic and nrsic demonstrated that the nanostructures of ...201424290890
the forensix evidence collection tube and its impact on dna preservation and recovery.biological samples are vulnerable to degradation from the time they are collected until they are analysed at the laboratory. biological contaminants, such as bacteria, fungi, and enzymes, as well as environmental factors, such as sunlight, heat, and humidity, can increase the rate of dna degradation. currently, dna samples are normally dried or frozen to limit their degradation prior to their arrival at the laboratory. in this study, the effect of the sample drying rate on dna preservation was i ...201324288659
the rulb gene of plasmid pww0 is a hotspot for the site-specific insertion of integron-like elements found in the chromosomes of environmental pseudomonas fluorescens group bacteria.the rulab operon of pseudomonas spp. confers fitness traits on the host and has been suggested to be a hotspot for insertion of mobile elements that carry avirulence genes. here, for the first time, we show that rulb on plasmid pww0 is a hotspot for the active site-specific integration of related integron-like elements (iles) found in six environmental pseudomonads (strains fh1-fh6). integration into rulb on pww0 occurred at position 6488 generating a 3 bp direct repeat. iles from fh1 and fh5 we ...201424286439
draft genome sequence of pseudomonas sp. strain cmaa 1215, a plant growth-promoting bacterium isolated from a brazilian mangrove.the aim of this study was to sequence the genome of the plant growth-promoting pseudomonas sp. strain cmaa 1215, an osmotolerant bacterium isolated from mangrove soil.201324285649
rate-limiting step analysis of the microbial desulfurization of dibenzothiophene in a model oil system.a mechanistic analysis of the various mass transport and kinetic steps in the microbial desulfurization of dibenzothiophene (dbt) by rhodococcus erythropolis igts8 in a model biphasic (oil-water), small-scale system was performed. the biocatalyst was distributed into three populations, free cells in the aqueous phase, cell aggregates and oil-adhered cells, and the fraction of cells in each population was measured. the power input per volume (p/v) and the impeller tip speed (vtip ) were identifie ...201324284557
rate-limiting step analysis of the microbial desulfurization of dibenzothiophene in a model oil system.a mechanistic analysis of the various mass transport and kinetic steps in the microbial desulfurization of dibenzothiophene (dbt) by rhodococcus erythropolis igts8 in a model biphasic (oil-water), small-scale system was performed. the biocatalyst was distributed into three populations, free cells in the aqueous phase, cell aggregates and oil-adhered cells, and the fraction of cells in each population was measured. the power input per volume (p/v) and the impeller tip speed (vtip ) were identifie ...201324284557
antifreeze protein activity in arctic cryoconite bacteria.fourteen arctic bacterial strains belonging to five genera, cryobacterium, leifsonia, polaromonas, pseudomonas, and subtercola isolated from sediments found in cryoconite holes of arctic glaciers, were subjected to screening for antifreeze proteins (afps). eight strains showed afp activity, and six strains of four species were further characterized. pseudomonas ficuserectae exhibited a high thermal hysteresis (th) activity. ice recrystallization inhibition (iri) activity was observed in most cul ...201424283367
structural and physiological analyses of the alkanesulphonate-binding protein (ssua) of the citrus pathogen xanthomonas citri.the uptake of sulphur-containing compounds plays a pivotal role in the physiology of bacteria that live in aerobic soils where organosulfur compounds such as sulphonates and sulphate esters represent more than 95% of the available sulphur. until now, no information has been available on the uptake of sulphonates by bacterial plant pathogens, particularly those of the xanthomonas genus, which encompasses several pathogenic species. in the present study, we characterised the alkanesulphonate uptak ...201324282519
dgge identification of microorganisms associated with borrelia burgdorferi sensu lato- or anaplasma phagocytophilum-infected ixodes ricinus ticks from northwest norway.ticks acquire a wide range of microorganisms as a natural part of their lifecycle. bacteria, viruses, and protozoa can be transmitted to ticks during feeding and free-living phases. dgge profiling is a molecular method to describe the microbial population associated with ticks and demonstrate some of the complexity and variety of tick-borne microorganisms. the present study profiled a total of 120 i. ricinus ticks, which were divided into three equally sized groups. we found that b. burgdorferi ...201324282414
plant growth-promoting bacteria: mechanisms and applications.the worldwide increases in both environmental damage and human population pressure have the unfortunate consequence that global food production may soon become insufficient to feed all of the world's people. it is therefore essential that agricultural productivity be significantly increased within the next few decades. to this end, agricultural practice is moving toward a more sustainable and environmentally friendly approach. this includes both the increasing use of transgenic plants and plant ...201224278762
molecular analysis of bacterial communities and detection of potential pathogens in a recirculating aquaculture system for scophthalmus maximus and solea senegalensis.the present study combined a dgge and barcoded 16s rrna pyrosequencing approach to assess bacterial composition in the water of a recirculating aquaculture system (ras) with a shallow raceway system (srs) for turbot (scophthalmus maximus) and sole (solea senegalensis). barcoded pyrosequencing results were also used to determine the potential pathogen load in the ras studied. samples were collected from the water supply pipeline (sup), fish production tanks (pro), sedimentation filter (sed), biof ...201324278329
phenotypic, immunologic, and clinical characteristics of patients with nontuberculous mycobacterial lung disease in korea.this study aimed to elucidate the phenotypic, immunologic, and clinical characteristics of korean patients with nontuberculous mycobacterial (ntm) lung disease and compare them with non-ntm bronchiectasis (be) patients.201324274658
acyl-homoserine lactone-based quorum sensing in the roseobacter clade: complex cell-to-cell communication controls multiple physiologies.bacteria have been widely reported to use quorum sensing (qs) systems, which employ small diffusible metabolites to coordinate gene expression in a population density dependent manner. in proteobacteria, the most commonly described qs signaling molecules are n-acyl-homoserine lactones (ahls). recent studies suggest that members of the abundant marine roseobacter lineage possess ahl-based qs systems and are environmentally relevant models for relating qs to ecological success. as reviewed here, t ...201324273537
discovery and characterization of a 5-hydroxymethylfurfural oxidase from methylovorus sp. strain mp688.in the search for useful and renewable chemical building blocks, 5-hydroxymethylfurfural (hmf) has emerged as a very promising candidate, as it can be prepared from sugars. hmf can be oxidized to 2,5-furandicarboxylic acid (fdca), which is used as a substitute for petroleum-based terephthalate in polymer production. on the basis of a recently identified bacterial degradation pathway for hmf, candidate genes responsible for selective hmf oxidation have been identified. heterologous expression of ...201424271187
solubility behavior of cyanophycin depending on lysine content.study of the synthesis of cyanophycin (cgp) in recombinant organisms focused for a long time mostly on the insoluble form of cgp, due to its easy purification and its putative use as a precursor for biodegradable chemicals. recently, another form of cgp, which, in contrast to the insoluble form, was soluble at neutral ph, became interesting due to its high lysine content, which was also assumed to be the reason for the solubility of the polymer. in this study, we demonstrate that lysine incorpor ...201424271185
functional screening and in vitro analysis reveal thioesterases with enhanced substrate specificity profiles that improve short-chain fatty acid production in escherichia coli.short-chain fatty acid (scfa) biosynthesis is pertinent to production of biofuels, industrial compounds, and pharmaceuticals from renewable resources. to expand on escherichia coli scfa products, we previously implemented a coenzyme a (coa)-dependent pathway that condenses acetyl-coa to a diverse group of short-chain fatty acyl-coas. to increase product titers and reduce premature pathway termination products, we conducted in vivo and in vitro analyses to understand and improve the specificity o ...201424271180
stimulation of nitrogen removal in the rhizosphere of aquatic duckweed by root exudate components.plants can stimulate bacterial nitrogen (n) removal by secretion of root exudates that may serve as carbon sources as well as non-nutrient signals for denitrification. however, there is a lack of knowledge about the specific non-nutrient compounds involved in this stimulation. here, we use a continuous root exudate-trapping system in two common aquatic duckweed species, spirodela polyrrhiza (hz1) and lemna minor (wx3), under natural and aseptic conditions. an activity-guided bioassay using denit ...201324271005
stimulation of nitrogen removal in the rhizosphere of aquatic duckweed by root exudate components.plants can stimulate bacterial nitrogen (n) removal by secretion of root exudates that may serve as carbon sources as well as non-nutrient signals for denitrification. however, there is a lack of knowledge about the specific non-nutrient compounds involved in this stimulation. here, we use a continuous root exudate-trapping system in two common aquatic duckweed species, spirodela polyrrhiza (hz1) and lemna minor (wx3), under natural and aseptic conditions. an activity-guided bioassay using denit ...201324271005
investigating pseudomonas putida-candida humicola interactions as affected by chelate fe(iii) in soil.microcalorimetric technique was applied to assess the toxic effect of edta-chelated trivalent iron on pseudomonas putida (p. putida) (bacterium), candida humicola (c. humicola) (fungus) and their mixture in sterilized soil. microbial growth rate constant k, total heat evolution q t, metabolic enthalpy ∆h met, mass specific heat rate j q/s, microbial biomass c and inhibitory ratio i were calculated. results showed that microcalorimetric indexes decreased with the increasing fe(iii)-edta complex c ...201424270965
rna-seq reveals the rna binding proteins, hfq and rsma, play various roles in virulence, antibiotic production and genomic flux in serratia sp. atcc 39006.serratia sp. atcc 39006 (s39006) is a gram-negative enterobacterium that is virulent in plant and animal models. it produces a red-pigmented trypyrrole secondary metabolite, prodigiosin (pig), and a carbapenem antibiotic (car), as well as the exoenzymes, pectate lyase and cellulase. secondary metabolite production in this strain is controlled by a complex regulatory network involving quorum sensing (qs). hfq and rsma (two rna binding proteins and major post-transcriptional regulators of gene exp ...201324267595
isotope fractionation associated with the biodegradation of 2- and 4-nitrophenols via monooxygenation pathways.monooxygenation is an important route of nitroaromatic compound (nac) biodegradation and it is widely found for cometabolic transformations of nacs and other aromatic pollutants. we investigated the c and n isotope fractionation of nitrophenol monooxygenation to complement the characterization of nac (bio)degradation pathways by compound-specific isotope analysis (csia). because of the large diversity of enzymes catalyzing monooxygenations, we studied the combined c and n isotope fractionation a ...201324266668
cluster and fold stability of e. coli isc-type ferredoxin.iron-sulfur clusters are essential protein prosthetic groups that provide their redox potential to several different metabolic pathways. formation of iron-sulfur clusters is assisted by a specialised machine that comprises, among other proteins, a ferredoxin. as a first step to elucidate the precise role of this protein in cluster assembly, we have studied the factors governing the stability and the dynamic properties of e. coli ferredoxin using different spectroscopic techniques. the cluster-lo ...201324265733
an underground tale: contribution of microbial activity to plant iron acquisition via ecological processes.iron (fe) deficiency in crops is a worldwide agricultural problem. plants have evolved several strategies to enhance fe acquisition, but increasing evidence has shown that the intrinsic plant-based strategies alone are insufficient to avoid fe deficiency in fe-limited soils. soil micro-organisms also play a critical role in plant fe acquisition; however, the mechanisms behind their promotion of fe acquisition remain largely unknown.201324265348
an underground tale: contribution of microbial activity to plant iron acquisition via ecological processes.iron (fe) deficiency in crops is a worldwide agricultural problem. plants have evolved several strategies to enhance fe acquisition, but increasing evidence has shown that the intrinsic plant-based strategies alone are insufficient to avoid fe deficiency in fe-limited soils. soil micro-organisms also play a critical role in plant fe acquisition; however, the mechanisms behind their promotion of fe acquisition remain largely unknown.201324265348
crystal structures and functional characterization of wild-type cyp101d1 and its active site mutants.although cyp101d1 and p450cam catalyze the same reaction at similar rates and share strikingly similar active site architectures, there are significant functional differences. cyp101d1 thus provides an opportunity to probe what structural and functional features must be shared and what features can differ but maintain the high catalytic efficiency. crystal structures of the cyanide complex of wild-type cyp101d1 and it active site mutants, d259n and t260a, have been determined. the conformational ...201324261604
crystal structure of the γ-hydroxymuconic semialdehyde dehydrogenase from pseudomonas sp. strainwbc-3, a key enzyme involved in para-nitrophenol degradation.para-nitrophenol (pnp) is a highly toxic compound with threats to mammalian health. the pnpe-encoded γ-hydroxymuconic semialdehyde dehydrogenase catalyzes the reduction of γ-hydroxymuconic semialdehyde to maleylacetate in pseudomonas sp. strain wbc-3, playing a key role in the catabolism of pnp to krebs cycle intermediates. however, the catalyzing mechanism by pnpe has not been well understood.201324252642
regulation of the expression level of transcription factor xyls reveals new functional insight into its induction mechanism at the pm promoter.xyls is the positive regulator of the inducible pm promoter, originating from pseudomonas putida, where the system controls a biochemical pathway involved in degradation of aromatic hydrocarbons, which also act as inducers. the xyls/pm positive regulator/promoter system is used for recombinant gene expression and the output from pm is known to be sensitive to the intracellular xyls concentration.201324252441
use of growing cells of pseudomonas aeruginosa for synthesis of the natural vanillin via conversion of isoeugenol.the great demand of people for consumption of natural additives resulted in producing natural vanillin. there are plant sources and chemical procedures for vanillin production but microbial bioconversions are being sought as a suitable alternative. in the present work, the ability to produce vanillin from isoeugenol was screened using growing cultures of various bacteria. among the 56 strains of bacteria isolated from the soil environments of iran, a gram-negative rod designated as strain ispc2 ...201124250410
transcriptomic fingerprinting of pseudomonas putida under alternative physiological regimes.pseudomonas putida kt2440 is a metabolically versatile soil bacterium useful both as a model biodegradative organism and as a host of catalytic activities of biotechnological interest. in this report, we present the high-resolution transcriptome of p. putida cultured on different carbon sources as revealed by deep sequencing of the corresponding rna pools. examination of the data from growth on substrates that are processed through distinct pathways (glucose, fructose, succinate and glycerol) re ...201324249296
the pseudomonas putida hska hybrid sensor kinase responds to redox signals and contributes to the adaptation of the electron transport chain composition in response to oxygen availability.pseudomonas putida has a branched aerobic electron transport that includes five terminal oxidases, each of which has different properties. the relative expression of each oxidase is carefully regulated to assemble the most suitable electron transport chain for the prevailing conditions. the hska hybrid sensor kinase participates in this control, but the signals to which hska responds were unknown. here, the influence of hska on the mrna abundance of genes coding for all terminal oxidases and for ...201324249291
Refresh
firstprev
...21222324252627282930...
nextlast
Displaying items 2301 - 2400 of 11585