Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| antigen-dependent versus -independent activation of invariant nkt cells during infection. | cd1d-reactive invariant nkt cells (inkt) play a vital role in determining the characteristics of immune responses to infectious agents. previous reports suggest that inkt cell activation during infection can be: 1) solely driven by cytokines from innate immune cells, 2) require microbial ag, or 3) require self-ag. in this study, we examined the role of ag receptor stimulation in inkt cells during several bacterial and viral infections. to test for ag receptor signaling, nur77(gfp) bac transgenic ... | 2014 | 24813205 |
| expanding the rpos/σs-network by rna sequencing and identification of σs-controlled small rnas in salmonella. | the rpos/σs sigma subunit of rna polymerase (rnap) controls a global adaptive response that allows many gram-negative bacteria to survive starvation and various stresses. σs also contributes to biofilm formation and virulence of the food-borne pathogen salmonella enterica serovar typhimurium (s. typhimurium). in this study, we used directional rna-sequencing and complementary assays to explore the σs-dependent transcriptome of s. typhimurium during late stationary phase in rich medium. this stud ... | 2014 | 24810289 |
| culture versus pcr for salmonella species identification in some dairy products and dairy handlers with special concern to its zoonotic importance. | a total of 200 samples of milk and dairy products as well as 120 samples of dairy handlers were randomly collected from different dairy farms and supermarkets in dakahlia governorate, egypt. the conventional cultural and serotyping methods for detection of salmonella in dairy products were applied and the results were compared with those obtained by molecular screening assay using (ttr sequence). the obtained results revealed that 21% of milk and dairy products (42/200) were positive for salmone ... | 2014 | 24808971 |
| contribution of increased isg15, isgylation and deregulated type i ifn signaling in usp18 mutant mice during the course of bacterial infections. | host genetics has a key role in susceptibility to salmonella typhimurium infection. we previously used n-ethyl-n-nitrosourea (enu) mutagenesis to identify a loss-of-function mutation within the gene ubiquitin-specific peptidase 18 (usp18(ity9)), which confers increased susceptibility to salmonella typhimurium. usp18 functions to regulate type i interferon (ifn) signaling and as a protease to remove isg15 from substrate proteins. usp18(ity9) mice are susceptible to infection with salmonella typhi ... | 2016 | 24807690 |
| in vitro evaluation of the mucin-adhesion ability and probiotic potential of lactobacillus mucosae lm1. | in this report, we characterized the probiotic potential of lactobacillus mucosae lm1, focusing on its in vitro mucin-adhesion abilities. | 2014 | 24807045 |
| development of an engineered bioluminescent reporter phage for the sensitive detection of viable salmonella typhimurium. | because foodborne illnesses continuously threaten public health, rapid and sensitive detection of pathogens in food has become an important issue. as an alternative to time-consuming and laborious conventional detection methods, a technique using recombinant reporter phages has been developed. here, we developed an advanced bioluminescent reporter phage spc32h-cdabe by inserting a bacterial luxcdabe operon into the salmonella temperate phage spc32h genome. whole spc32h genome sequencing enabled ... | 2014 | 24806327 |
| inflammasome activation causes dual recruitment of nlrc4 and nlrp3 to the same macromolecular complex. | pathogen recognition by nucleotide-binding oligomerization domain-like receptor (nlr) results in the formation of a macromolecular protein complex (inflammasome) that drives protective inflammatory responses in the host. it is thought that the number of inflammasome complexes forming in a cell is determined by the number of nlrs being activated, with each nlr initiating its own inflammasome assembly independent of one another; however, we show here that the important foodborne pathogen salmonell ... | 2014 | 24803432 |
| gastrointestinal microbes interact with canine adipose-derived mesenchymal stem cells in vitro and enhance immunomodulatory functions. | mesenchymal stem cells (mscs) are somatic, multipotent stromal cells with potent immunomodulatory and regenerative properties. although mscs have pattern recognition receptors and are modulated by toll-like receptor ligands, msc-microbial interactions are poorly defined. the objectives of this study were to determine the effect of bacterial association on msc function. we hypothesized that gastrointestinal bacteria associate with mscs and alter their immunomodulatory properties. the effect of ms ... | 2014 | 24803072 |
| determination of sulfotransferase forms involved in the metabolic activation of the genotoxicant 1-hydroxymethylpyrene using bacterially expressed enzymes and genetically modified mouse models. | 1-methylpyrene, a carcinogenic polycyclic aromatic hydrocarbon, forms benzylic dna adducts, in particular n2-(1-methylpyrenyl)-2'-deoxyguanosine, in mice and rats. it is bioactivated via 1-hydroxymethylpyrene (1-hmp) to electrophilic 1-sulfooxymethylpyrene (1-smp). in this study, we explored the role of individual mouse sulfotransferase (sult) forms in this activation. first, we showed that all nine mouse sults tested were able to activate 1-hmp to a mutagen in the his- salmonella typhimurium re ... | 2014 | 24802129 |
| characterization of the invasiveness of monophasic and aphasic salmonella typhimurium strains in 1-day-old and point-of-lay chickens. | egg-related outbreaks of salmonellosis are a significant health concern. although salmonella enteritidis (se) is the major egg-associated serotype, salmonella typhimurium (st) can also infect the hen's reproductive tract and contaminate eggs. recently, monophasic and aphasic variants of st have been reported with increased frequency in europe, and the isolation of these variants from laying flocks triggers the same legislative restrictions associated with biphasic st strains. however, little is ... | 2014 | 24802091 |
| biochemical and antimicrobial activity of phloretin and its glycosilated derivatives present in apple and kumquat. | phloretin and its glycosylated derivatives (phlorizin and phloretin 3',5'-di-c-glucoside) are dihydrochalcones that have many interesting biological properties. the results obtained showed that the dihydrochalcones are able to inhibit growth of gram positive bacteria, in particular staphylococcus aureus atcc 6538, listeria monocytogenes atcc 13932 and methicillin-resistant s. aureus clinical strains. moreover, phloretin is active also against the gram negative bacteria salmonella typhimurium atc ... | 2014 | 24799241 |
| effect of atmospheric pressure dielectric barrier discharge plasma on the biological activity of naringin. | the biological activity of naringin treated with atmospheric pressure plasma was evaluated to investigate whether exposure to plasma can be used as a method to improve the biological activity of natural materials. naringin was dissolved in methanol (at 500 ppm) and transferred to a container. a dielectric barrier discharge (dbd) (250 w, 15 khz, ambient air) was then generated. treatment with the plasma for 20 min increased the radical-scavenging activity, frap value, and the total phenolic compo ... | 2014 | 24799234 |
| nutritional and metabolic requirements for the infection of hela cells by salmonella enterica serovar typhimurium. | salmonella is the causative agent of a spectrum of human and animal diseases ranging from gastroenteritis to typhoid fever. it is a food--and water--borne pathogen and infects via ingestion followed by invasion of intestinal epithelial cells and phagocytic cells. in this study we employed a mutational approach to define the nutrients and metabolic pathways required by salmonella enterica serovar typhimurium during infection of a human epithelial cell line (hela). we deleted the key glycolytic ge ... | 2014 | 24797930 |
| decay of genetic markers for fecal bacterial indicators and pathogens in sand from lake superior. | beach sands impact water quality and pathogen loads, however, the comparative decay of the fecal indicator bacteria (fib) enterococcus spp. and escherichia coli, and pathogens in freshwater sand have not been examined. in this study, freshwater sand microcosms were inoculated with sewage and pure cultures of bacterial pathogens to compare relative decay rates. the abundance of culturable enterococcus spp. and e. coli, genetic markers for enterococcus spp. (entero1), total bacteroides (allbac), a ... | 2014 | 24793108 |
| phenolic content and antioxidant and antimutagenic activities in tomato peel, seeds, and byproducts. | the phenolic content and antioxidant and antimutagenic activities from the peel and seeds of different tomato types (grape, cherry, bola and saladette type), and simulated tomato industrial byproducts, were studied. methanolic extracts were used to quantify total phenolic content, groups of phenolic compounds, antioxidant activities, and the profile of phenolic compounds (by hplc-dad). antimutagenic activity was determined by salmonella typhimurium assay. the total phenolic content and antioxida ... | 2014 | 24792924 |
| potential of goat probiotic to bind mutagens. | the mutagen binding ability of the goat probiotics (lactobacillus reuteri ddl 19, lactobacillus alimentarius ddl 48, enterococcus faecium dde 39, and bifidobacterium bifidum ddba) was evaluated. the oral administration of these probiotics reduced fecal mutagens and intestinal cancer markers in goats. secondly, the effects of probiotics against the mutagenesis induced by sodium azide (sa), and benzopyrene (b[α]p) by performing the modified ames test using salmonella typhimurium ta 100 was investi ... | 2014 | 24785349 |
| a sequence-based approach for prediction of csra/rsma targets in bacteria with experimental validation in pseudomonas aeruginosa. | csra/rsma homologs are an extensive family of ribonucleic acid (rna)-binding proteins that function as global post-transcriptional regulators controlling important cellular processes such as secondary metabolism, motility, biofilm formation and the production and secretion of virulence factors in diverse bacterial species. while direct messenger rna binding by csra/rsma has been studied in detail for some genes, it is anticipated that there are numerous additional, as yet undiscovered, direct ta ... | 2014 | 24782516 |
| physicochemical, mechanical, and molecular properties of nonlysogenic and p22-lysogenic salmonella typhimurium treated with citrus oil. | the aim of this study was to evaluate the phenotypic and genotypic properties of nonlysogenic salmonella typhimurium (st(p22-)) and lysogenic salmonella typhimurium (st(p22+)) in the presence of sublethal concentrations (slc2d) of citrus essential oils (ceos), which were used to evaluate antimicrobial susceptibility, cell surface hydrophobicity, autoaggregation ability, bacterial motility, lysogenic conversion, gene expression patterns, and antibiofilm formation. the slc2d values of non-heat-tre ... | 2014 | 24780330 |
| salmonella internalization in mung bean sprouts and pre- and postharvest intervention methods in a hydroponic system. | mung bean sprouts, typically consumed raw or minimally cooked, are often contaminated with pathogens. internalized pathogens pose a high risk because conventional sanitization methods are ineffective for their inactivation. the studies were performed (i) to understand the potential of internalization of salmonella in mung bean sprouts under conditions where the irrigation water was contaminated and (ii) to determine if pre- and postharvest intervention methods are effective in inactivating the i ... | 2014 | 24780329 |
| pathogen exclusion properties of canine probiotics are influenced by the growth media and physical treatments simulating industrial processes. | manufacturing process used in preparation of probiotic products may alter beneficial properties of probiotics. the effect of different growth media and inactivation methods on the protective properties of canine-originated probiotic bacteria against adhesion of canine enteropathogens was investigated. | 2014 | 24779583 |
| mass mortality of eurasian tree sparrows (passer montanus) from salmonella typhimurium dt40 in japan, winter 2008-09. | an outbreak of salmonellosis in wild passerines caused mass mortality of eurasian tree sparrows (passer montanus) in hokkaido, japan, 2005-06; however, the etiology was poorly understood. in winter 2008-09, sparrow mortality again occurred in hokkaido, and 202 deaths in 100 incidents at 94 sites were reported. we conducted a comprehensive investigation to evaluate the cause and impact on sparrow populations. we collected 26 carcasses at 13 sites, including a zoological park. in addition, salmone ... | 2014 | 24779465 |
| the focal complex of epithelial cells provides a signalling platform for interleukin-8 induction in response to bacterial pathogens. | bacterial pathogens can induce an inflammatory response from epithelial tissues due to secretion of the pro-inflammatory chemokine interleukin-8 (il-8). many bacterial pathogens manipulate components of the focal complex (fc) to induce signalling events in host cells. we examined the interaction of several bacterial pathogens with host cells, including campylobacter jejuni, to determine if the fc is required for induction of chemokine signalling in response to bacterial pathogens. our data indic ... | 2014 | 24779413 |
| the salmonella effector stea contributes to the control of membrane dynamics of salmonella-containing vacuoles. | salmonella enterica serovar typhimurium is a bacterial pathogen causing gastroenteritis in humans and a typhoid-like systemic disease in mice. s. typhimurium virulence is related to its capacity to multiply intracellularly within a membrane-bound compartment, the salmonella-containing vacuole (scv), and depends on type iii secretion systems that deliver bacterial effector proteins into host cells. here, we analyzed the cellular function of the salmonella effector stea. we show that, compared to ... | 2014 | 24778114 |
| assessment of mutagenic, genotoxic, and cytotoxic potential of water samples of harike wetland: a ramsar site in india using different ex vivo biological systems. | harike is a wetland of international importance under the ramsar convention. the present study entails the investigation of mutagenic, genotoxic and cytotoxic effect of surface water samples collected from five different areas of the harike wetland by using the histidine reversion point mutation assay in salmonella typhimurium (ta98 and ta100) strain with or without s9, bioluminescence mutagenicity assay using vibrio harveyi (a16) strain, plasmid-nicking assay using pbr322 and 3(4,5-dimethyl-thi ... | 2014 | 24777664 |
| in silico detection and typing of plasmids using plasmidfinder and plasmid multilocus sequence typing. | in the work presented here, we designed and developed two easy-to-use web tools for in silico detection and characterization of whole-genome sequence (wgs) and whole-plasmid sequence data from members of the family enterobacteriaceae. these tools will facilitate bacterial typing based on draft genomes of multidrug-resistant enterobacteriaceae species by the rapid detection of known plasmid types. replicon sequences from 559 fully sequenced plasmids associated with the family enterobacteriaceae i ... | 2014 | 24777092 |
| transmission parameters estimated for salmonella typhimurium in swine using susceptible-infectious-resistant models and a bayesian approach. | transmission models can aid understanding of disease dynamics and are useful in testing the efficiency of control measures. the aim of this study was to formulate an appropriate stochastic susceptible-infectious-resistant/carrier (sir) model for salmonella typhimurium in pigs and thus estimate the transmission parameters between states. | 2014 | 24774444 |
| phytochemistry and preliminary assessment of the antibacterial activity of chloroform extract of amburana cearensis (allemão) a.c. sm. against klebsiella pneumoniae carbapenemase-producing strains. | the chloroform extract of the stem bark of amburana cearensis was chemically characterized and tested for antibacterial activity.the extract was analyzed by gas chromatography and mass spectrometry. the main compounds identified were 4-methoxy-3-methylphenol (76.7%), triciclene (3.9%), α -pinene (1.0%), β -pinene (2.2%), and 4-hydroxybenzoic acid (3.1%). preliminary antibacterial tests were carried out against species of distinct morphophysiological characteristics: escherichia coli, salmonella ... | 2014 | 24772183 |
| evaluation the surface antigen of the salmonella typhimurium atcc 14028 ghosts prepared by "slrp". | recently, bacterial ghosts (bgs) were prepared using a protocol based on critical chemical concentrations. it has been given the name "sponge like" (sl) protocol and used in its reduced form "sponge like reduced protocol" (slrp). while specific antibody for salmonella is available on the market under the commercial names (of some kits) such as febrile antigen kit (n.s. bio-tec), we used the described kit to investigate the validity of the slrp. in this study, using slrp we succeeded to prepare s ... | 2014 | 24772035 |
| assessment of titanium dioxide nanoparticle effects in bacteria: association, uptake, mutagenicity, co-mutagenicity and dna repair inhibition. | due to their unique properties, the use of nanoparticles (nps) is expanding; these same properties may affect their potential risk to humans. however, standard methods for genotoxicity assessment may not be adequate for nps; altered tests reported here have been developed to address perceived inadequacies. the bacterial reverse mutation assay is an essential part of the battery of tests to determine genotoxicity. the utility of this test for assessing nps is currently questioned, due to negative ... | 2014 | 24769488 |
| diarrhoeagenic escherichia coli and salmonellae in calves and lambs in kashmir absence, prevalence and antibiogram. | polymerase chain reaction assays and culture were used to investigate 728 faecal samples from 404 calves (286 diarrhoeic, 118 healthy) and 324 lambs (230 diarrhoeic, 94 healthy) in kashmir, india, for the presence of enterotoxigenic escherichia coli (etec), enteroaggregative e. coli (eaec), diffusely adherent e. coli (daec) and salmonellae. antimicrobial sensitivity patterns were also investigated. in total, 23 etec isolates were obtained from the diarrhoeic calves and 12 from diarrhoeic lambs. ... | 2013 | 24761734 |
| non-typhoidal salmonella: an unusual cause of spontaneous bacterial peritonitis in decompensated cirrhosis. | salmonella typhimurium, a non-typhoidal salmonella, is an unusual cause of spontaneous bacterial peritonitis (sbp). it is usually reported in asymptomatic patients with normal or high ascitic fluid protein levels with underlying immunosuppression, as high opsonic activity in the ascitic fluid of these patients protects them from the usual organisms causing spontaneous bacterial peritonitis, unless they are exposed to a particularly virulent organism like salmonella. we report a case of culture-p ... | 2014 | 24759352 |
| fibrates ameliorate the course of bacterial sepsis by promoting neutrophil recruitment via cxcr2. | bacterial sepsis results in high mortality rates, and new therapeutics to control infection are urgently needed. here, we investigate the therapeutic potential of fibrates in the treatment of bacterial sepsis and examine their effects on innate immunity. fibrates significantly improved the survival from sepsis in mice infected with salmonella typhimurium, which was paralleled by markedly increased neutrophil influx to the site of infection resulting in rapid clearance of invading bacteria. as a ... | 2014 | 24755316 |
| emergence of clinical salmonella enterica serovar typhimurium isolates with concurrent resistance to ciprofloxacin, ceftriaxone, and azithromycin. | salmonella infection is an important public health issue for which the needs of antimicrobial treatment are increasing. a total of 546 human clinical s. enterica serovar typhimurium isolates were recovered from patients in hospitals in china during the period of 2005 to ∼ 2011. twenty percent of the isolates exhibited resistance to ciprofloxacin, and 4% were resistant to ceftriaxone. importantly, for the first time, 12 (2%) s. typhimurium isolates resistant to both ciprofloxacin and ceftriaxone ... | 2014 | 24752251 |
| evaluations of the mutagenicity of a pigment extract from bulb culture of hippeastrum reticulatum. | the use of anthocyanins in food products as colorants has been limited because of their instability toward alkaline ph and high temperature. this study aimed to determine color stability and mutagenicity of the anthocyanin-based pigment extract from bulb cultures of hippeastrum (hippeastrum reticulatum). the pigment extract retained its reddish-orange color under alkaline conditions (⩽ph 11) and was stable up to 6 h at 95 °c. the mutagenicity of the extract was evaluated in vitro and in vivo. hi ... | 2014 | 24751972 |
| sub-inhibitory fosmidomycin exposures elicits oxidative stress in salmonella enterica serovar typhimurium lt2. | fosmidomycin is a time-dependent nanomolar inhibitor of methylerythritol phosphate (mep) synthase, which is the enzyme that catalyzes the first committed step in the mep pathway to isoprenoids. importantly, fosmidomycin is one of only a few mep pathway-specific inhibitors that exhibits antimicrobial activity. most inhibitors identified to date only exhibit activity against isolated pathway enzymes. the mep pathway is the sole route to isoprenoids in many bacteria, yet has no human homologs. the ... | 2014 | 24751777 |
| giardia duodenalis cathepsin b proteases degrade intestinal epithelial interleukin-8 and attenuate interleukin-8-induced neutrophil chemotaxis. | giardia duodenalis (syn. g. intestinalis, g. lamblia) infections are a leading cause of waterborne diarrheal disease that can also result in the development of postinfectious functional gastrointestinal disorders via mechanisms that remain unclear. parasite numbers exceed 10(6) trophozoites per centimeter of gut at the height of an infection. yet the intestinal mucosa of g. duodenalis-infected individuals is devoid of signs of overt inflammation. g. duodenalis infections can also occur concurren ... | 2014 | 24733096 |
| selected lactic acid-producing bacterial isolates with the capacity to reduce salmonella translocation and virulence gene expression in chickens. | probiotics have been used to control salmonella colonization/infection in chickens. yet the mechanisms of probiotic effects are not fully understood. this study has characterized our previously-selected lactic acid-producing bacterial (lab) isolates for controlling salmonella infection in chickens, particularly the mechanism underlying the control. | 2014 | 24728092 |
| evaluation of mutagenic and genotoxic activities of lobeline and its modulation on genomic instability induced by ethanol. | lobeline is a natural alkaloid derived from lobelia inflata that has been investigated as a clinical candidate for the treatment of alcoholism. in a pre-clinical trial, lobeline decreased the preference for and consumption of ethanol, due to the modulation of the nicotinic acetylcholine receptor. however, the interaction between lobeline and ethanol is poorly known and thus there are safety concerns. the present study was conducted to evaluate the mutagenic and genotoxic effects of lobeline and ... | 2014 | 24727238 |
| the sdia-regulated gene srge encodes a type iii secreted effector. | salmonella enterica serovar typhimurium is a food-borne pathogen that causes severe gastroenteritis. the ability of salmonella to cause disease depends on two type iii secretion systems (t3sss) encoded in two distinct salmonella pathogenicity islands, 1 and 2 (spi1 and spi2, respectively). s. typhimurium encodes a solo luxr homolog, sdia, which can detect the acyl-homoserine lactones (ahls) produced by other bacteria and upregulate the rck operon and the srge gene. srge is predicted to encode a ... | 2014 | 24727228 |
| murine solid tumours as a novel model to study bacterial biofilm formation in vivo. | bacteria of many species are able to invade and colonize solid tumours in mice. we have focused on salmonella enterica serovar typhimurium. detailed analysis revealed that such tumour-invading salmonella form biofilms, thus providing a versatile in vivo test system for studying bacterial phenotypes and host-pathogen interactions. it appears that biofilm formation by s. typhimurium is induced as a defence against the immune system of the host, and in particular against neutrophils. further, we ex ... | 2014 | 24724621 |
| antimicrobial resistance in bacteria isolated from pigeons in poland. | the present study investigated the drug-resistance to the selected antibiotics in escherichia coli, salmonella typhimurium and beta-haemolytic coagulase-positive staphylococci isolated from pigeons bred in poland. in the case of e. coli, tetracyclines and amoxicillin were least effective. in the staphylococci, the highest resistance was detected for oxytetracycline and quinolones and 5% were resistant to methicillin. the lowest drug-resistance was reported for salmonella typhimurium. | 2014 | 24724486 |
| assessment of conjugal transfer of antibiotic resistance genes in salmonella typhimurium exposed to bile salts. | this study was designed to evaluate the transfer potential of antibiotic resistance genes in antibiotic-resistant salmonella typhimurium (s. typhimurium(r)) in the presence of bile salts. the resistance of s. typhimurium(r) to ampicillin, kanamycin, and tetracycline was increased by 64-, 64-, and 512-fold, respectively. the highest transfer frequency from s. typhimurium(r) to escherichia coli was observed at the bile salt concentration of 160 μg/ml (3.8 × 10(-3) transferrants/cells). the express ... | 2014 | 24723101 |
| functional characterization of the type iii secretion atpase ssan encoded by salmonella pathogenicity island 2. | a type iii secretion system (t3ss) is utilized by a large number of gram-negative bacteria to deliver effectors directly into the cytosol of eukaryotic host cells. one essential component of a t3ss is an atpase that catalyzes the unfolding of proteins, which is followed by the translocation of effectors through an injectisome. here we demonstrate a functional role of the atpase ssan, a component of salmonella pathogenicity island 2 t3ss (t3ss-2) in salmonella enterica serovar typhimurium. ssan h ... | 2014 | 24722491 |
| simultaneous and rapid detection of salmonella typhi, bacillus anthracis, and yersinia pestis by using multiplex polymerase chain reaction (pcr). | salmonella typhi, bacillus anthracis, and yersinia pestis are some serious human pathogens, which their early diagnosis is of great importance. salmonella typhi, bacillus anthracis, and yersinia pestis cause typhoid fever, anthrax, and plague respectively. these bacteria can be used to make biologic weapons. | 2013 | 24719692 |
| integrative analysis of salmonellosis in israel reveals association of salmonella enterica serovar 9,12:l,v:- with extraintestinal infections, dissemination of endemic s. enterica serovar typhimurium dt104 biotypes, and severe underreporting of outbreaks. | salmonella enterica is the leading etiologic agent of bacterial food-borne outbreaks worldwide. this ubiquitous species contains more than 2,600 serovars that may differ in their host specificity, clinical manifestations, and epidemiology. to characterize salmonellosis epidemiology in israel and to study the association of nontyphoidal salmonella (nts) serovars with invasive infections, 48,345 salmonella cases reported and serotyped at the national salmonella reference center between 1995 and 20 ... | 2014 | 24719441 |
| a comparative study of the sensitivities of salmonella typhimurium strains ta 98, ta 100 and ta 102 to hospital waste waters. | hospitals are a release source of many chemical compounds in their wastewaters. in the present study salmonella typhimurium strains ta 98, ta 100 and ta 102 were analyzed for their sensitivity to hospital waste waters. the results of the study showed that hospital waste waters consists of mutagens causing frame shift mutations and base pair substitutions and amongst the three strains used in this study, ta 102 was most effective which along with ta 98 can be used for quick assessment of genotoxi ... | 2014 | 24718501 |
| persistent salmonellosis causes pancreatitis in a murine model of infection. | pancreatitis, a known risk factor for the development of pancreatic ductal adenocarcinoma, is a serious, widespread medical condition usually caused by alcohol abuse or gallstone-mediated ductal obstruction. however, many cases of pancreatitis are of an unknown etiology. pancreatitis has been linked to bacterial infection, but causality has yet to be established. here, we found that persistent infection of mice with the bacterial pathogen salmonella enterica serovar typhimurium (s. typhimurium) ... | 2014 | 24717768 |
| removal of the phage-shock protein pspb causes reduction of virulence in salmonella enterica serovar typhimurium independently of nramp1. | the phage-shock protein (psp) system is believed to manage membrane stress in all enterobacteriaceae and has recently emerged as being important for virulence in several pathogenic species of this phylum. the core of the psp system consists of the pspa-d operon and the distantly located pspg gene. in salmonella enterica serovar typhimurium (s. typhimurium), it has recently been reported that pspa is essential for systemic infection of mice, but only in nramp1(+) mice, signifying that attenuation ... | 2014 | 24713356 |
| regulation of 5-oxo-ete synthesis by nitric oxide in human polymorphonuclear leucocytes upon their interaction with zymosan and salmonella typhimurium. | in the present study we have presented data on the regulation of lt (leukotriene) and 5-oxo-ete (5-oxo-6,8,11,14-eicosatetraenoic acid) syntheses in human neutrophils upon interaction with oz (opsonized zymosan) or salmonella typhimurium. priming of neutrophils with pma (phorbol 12-myristate 13-acetate) and lps (lipopolysaccharide) elicits 5-oxo-ete formation in neutrophils exposed to oz, and the addition of aa (arachidonic acid) significantly increases 5-oxo-ete synthesis. we found that no (nit ... | 2014 | 24712762 |
| cd11b+ ly6chi ly6g- immature myeloid cells recruited in response to salmonella enterica serovar typhimurium infection exhibit protective and immunosuppressive properties. | immature myeloid cells in bone marrow are a heterogeneous population of cells that, under normal conditions, provide tissues with protective cell types such as granulocytes and macrophages. under certain pathological conditions, myeloid cell homeostasis is altered and immature forms of these cells appear in tissues. murine immature myeloid cells that express cd11b and ly6c or ly6g (two isoforms of gr-1) have been associated with immunosuppression in cancer (in the form of myeloid-derived suppres ... | 2014 | 24711563 |
| arginine and vitamin e improve the immune response after a salmonella challenge in broiler chicks. | two experiments were conducted to evaluate the effects of arg, vitamin e (ve), and mannanoligosaccharide (mos) on the immune response and clearance of salmonella in broiler chickens. in each experiment, 1-d-old chicks (n = 160) were randomly distributed into 4 groups: antibiotic-free diet (negative control, ctl-), antibiotic-supplemented diet (positive control, ctl+), antibiotic free-diet plus arg and ve (ave), or antibiotic-free diet plus arg, ve, and mos (avm). birds were orally challenged wit ... | 2014 | 24706965 |
| effects of dietary clays on performance and intestinal mucus barrier of broiler chicks challenged with salmonella enterica serovar typhimurium and on goblet cell function in vitro. | in vivo and in vitro experiments were conducted to test for beneficial effects of dietary clays on broiler chicks challenged with salmonella enterica serovar typhimurium and to explore potential mechanisms. first, two hundred forty 1-d-old male broilers (initial bw: 41.6 ± 0.4 g) were allotted in a 2 × 4 factorial arrangement in a randomized complete block design. there were 2 infection treatments (with or without salmonella) and 4 diets: basal (bas), 0.3% smectite a (sma), 0.3% smectite b, and ... | 2014 | 24706960 |
| flgn is required for flagellum-based motility by bacillus subtilis. | the assembly of the bacterial flagellum is exquisitely controlled. flagellar biosynthesis is underpinned by a specialized type iii secretion system that allows export of proteins from the cytoplasm to the nascent structure. bacillus subtilis regulates flagellar assembly using both conserved and species-specific mechanisms. here, we show that yvyg is essential for flagellar filament assembly. we define yvyg as an orthologue of the salmonella enterica serovar typhimurium type iii secretion system ... | 2014 | 24706744 |
| analysis of factors that affect flgm-dependent type iii secretion for protein purification with salmonella enterica serovar typhimurium. | the flgm protein is secreted in response to flagellar hook-basal body secretion and can be used as a secretion signal to direct selected protein secretion via the flagellar type iii secretion (t3s) system [h. m. singer, m. erhardt, a. m. steiner, m. m. zhang, d. yoshikami, g. bulaj, b. m. olivera, and k. t. hughes, mbio 3(3):e00115-12, 2012, http://dx.doi.org/10.1128/mbio.00115-12]. conditions known to affect flagellar gene expression, flgm stability, and flagellar t3s were tested either alone o ... | 2014 | 24706743 |
| impact of quinolone-resistance acquisition on biofilm production and fitness in salmonella enterica. | to investigate the potential relationship between quinolone resistance and biofilm production in a collection of salmonella enterica clinical isolates and in s. enterica serovar typhimurium serial mutants with increasing resistance to ciprofloxacin. | 2014 | 24706735 |
| identification of traf6 as a ubiquitin ligase engaged in the ubiquitination of sopb, a virulence effector protein secreted by salmonella typhimurium. | the phosphoinositide phosphatase sopb is one of the effectors injected by salmonella typhimurium (s.typhimurium) that diversifies its function through a ubiquitin-dependent differential localization. however, it is unclear which e3 ubiquitin ligase is responsible for ubiquitination of sopb. based on the e1-e2-e3 trio of enzymes responsible for the ubiquitin activation and translocation to substrate proteins, we constructed an in vitro assay of sopb ubiquitination. using this assay, we purified a ... | 2014 | 24704445 |
| complete genome sequence of a multidrug-resistant salmonella enterica serovar typhimurium var. 5- strain isolated from chicken breast. | 2014 | 24699967 | |
| successful data recovery from oscillation photographs containing strong polycrystalline diffraction rings from an unknown small-molecule contaminant: preliminary structure solution of salmonella typhimurium pyridoxal kinase (pdxk). | pyridoxal kinase (pdxk; ec 2.7.1.35) belongs to the phosphotransferase family of enzymes and catalyzes the conversion of the three active forms of vitamin b6, pyridoxine, pyridoxal and pyridoxamine, to their phosphorylated forms and thereby plays a key role in pyridoxal 5'-phosphate salvage. in the present study, pyridoxal kinase from salmonella typhimurium was cloned and overexpressed in escherichia coli, purified using ni-nta affinity chromatography and crystallized. x-ray diffraction data wer ... | 2014 | 24699755 |
| [efficacy of immune responses induced by anti-caries dna vaccine-loaded bacterial ghost in mice]. | to develop an anti-caries dna vaccine-loaded salmonella typhimurium (st) ghost and enhance the efficacy of immune responses induced by anti-caries dna vaccine via mucosal route. | 2014 | 24697886 |
| [prevalence and characterization of salmonella spp. from foods in south china]. | in this study, we studied systematically the prevalence of salmonella in foods from various parts of south china. isolated strains were characterized by serotyping and enterobacterial repetitive intergenic consensus polymerase chain reaction (eric-pcr), to provide data support for effectively tracing the source of salmonella and controlling food contamination. | 2013 | 24697105 |
| isolation and validation of an endogenous fluorescent nucleoid reporter in salmonella typhimurium. | in this study we adapted a mud-based delivery system to construct a random yfp reporter gene (encoding the yellow fluorescent protein) insertion library in the genome of salmonella typhimurium lt2, and used fluorescence activated cell sorting and fluorescence microscopy to screen for translational fusions that were able to clearly and specifically label the bacterial nucleoid. two such fusions were obtained, corresponding to a translational yfp insertion in iscr and iolr, respectively. both fusi ... | 2014 | 24695782 |
| chitinase 3-like 1 synergistically activates il6-mediated stat3 phosphorylation in intestinal epithelial cells in murine models of infectious colitis. | chitinase 3-like 1 (chi3l1) is an inducible molecule on intestinal epithelial cells during the development of inflammatory bowel disease. | 2014 | 24694795 |
| rig-i detects mrna of intracellular salmonella enterica serovar typhimurium during bacterial infection. | the cytoplasmic helicase rig-i is an established sensor for viral 5'-triphosphorylated rna species. recently, rig-i was also implicated in the detection of intracellular bacteria. however, little is known about the host cell specificity of this process and the bacterial pathogen-associated molecular pattern (pamp) that activates rig-i. here we show that rna of salmonella enterica serovar typhimurium activates production of beta interferon in a rig-i-dependent fashion only in nonphagocytic cells. ... | 2014 | 24692634 |
| phenotypic characterization of salmonella ryhb-1 mutations that modulate target regulation. | recently, we reported the global regulatory roles of the two small rnas in salmonella typhimurium, ryhb-1 and ryhb-2. however, the genetic basis of the srna-target interactions remains unknown. to identify the nucleotides of ryhb-1 that are functionally important for its regulatory actions, we introduced random single-point mutations into ryhb-1 gene on the chromosome of salmonella typhimurium carrying a sodb-lacz translational fusion by an error-prone pcr method. we reasoned that mutants expres ... | 2014 | 24691548 |
| general regression neural network model for behavior of salmonella on chicken meat during cold storage. | a study was undertaken to investigate and model behavior of salmonella on chicken meat during cold storage at constant temperatures. chicken meat (white, dark, or skin) portions (0.75 cm(3)) were inoculated with a single strain of salmonella typhimurium dt104 (2.8 log) followed by storage for 0 to 8 d at -8, 0, 8, 12, 14, or 16 °c for model development and at -4, 4, 10, or 14 °c for model validation. a general regression neural network model was developed with commercial software. performance of ... | 2014 | 24689996 |
| cloning and characterization of a novel fold-type i branched-chain amino acid aminotransferase from the hyperthermophilic archaeon thermococcus sp. cku-1. | we successfully cloned a novel branched-chain amino acid aminotransferase (ts-bcat; ec 2.6.1.42) gene from the thermococcus sp. cku-1 genome and expressed it in the soluble fraction of escherichia coli rosetta (de3) cells. ts-bcat is a homodimer with an apparent molecular mass of approximately 92 kda. the primary structure of ts-bcat showed high homology with the fold-type i, subgroup i aminotransferases, but showed little homology with bcats known to date, i.e., those of escherichia coli and sa ... | 2014 | 24687296 |
| diagnostic exercise: high mortality in a flock of chukar partridge chicks (alectoris chukar) in california. | mortality of 20% of a flock of 1000 chukar partridge chicks occurred over a 6-week period in northern california from august to september 2012. affected birds were 2 to 42 days old and died without premonitory clinical signs or after showing ruffled feathers and anorexia for 24 to 72 hours. three carcasses were submitted for necropsy, 2 birds had hemorrhagic tracheitis grossly, and all 3 had lymphoplasmacytic and histiocytic myocarditis with myocardial necrosis microscopically. the differential ... | 2015 | 24686386 |
| antimicrobial susceptibility of salmonella enterica isolates from healthy breeder and broiler flocks in portugal. | three hundred and thirty-three isolates representing 40 different serotypes of salmonella enterica, recovered from environmental and faecal samples of breeder and broiler flocks from 2009 to 2011, were studied. antimicrobial susceptibility was determined by measuring the minimal inhibitory concentration of 11 antimicrobials using the agar dilution method. salmonella havana, s. enteritidis and s. mbandaka were the most common serotypes isolated from broiler flocks, while s. enteritidis was the co ... | 2014 | 24685467 |
| comparative efficacy of a multi-epitope dna vaccine via intranasal, peroral, and intramuscular delivery against lethal toxoplasma gondii infection in mice. | toxoplasmosis is an important zoonosis, being a cause of congenital disease and abortion in animals and humans. dna vaccination as a promising vaccine remains a challenge for an improved delivery system. | 2014 | 24685150 |
| reductions of shiga toxin-producing escherichia coli and salmonella typhimurium on beef trim by lactic acid, levulinic acid, and sodium dodecyl sulfate treatments. | studies were done at 21 °c to determine the bactericidal activity of lactic acid, levulinic acid, and sodium dodecyl sulfate (sds) applied individually and in combination on shiga toxin-producing escherichia coli (stec) in pure culture and to compare the efficacy of lactic acid and levulinic acid plus sds treatments applied by spray or immersion to inactivate stec and salmonella (10(7) cfu/cm2) on beef trim pieces (10 by 10 by 7.5 cm). application of 3% lactic acid for 2 min to pure cultures was ... | 2014 | 24680063 |
| investigation of the antimicrobial activity of bacillus licheniformis strains isolated from retail powdered infant milk formulae. | this study investigated the potential antimicrobial activity of ten bacillus licheniformis strains isolated from retail infant milk formulae against a range of indicator (lactococcus lactis, lactobacillus bulgaricus and listeria innocua) and clinically relevant (listeria monocytogenes, staphylococcus aureus, streptococcus agalactiae, salmonella typhimurium and escherichia coli) microorganisms. deferred antagonism assays confirmed that all b. licheniformis isolates show antimicrobial activity aga ... | 2014 | 24676765 |
| probiotic potential of lactobacillus plantarum ld1 isolated from batter of dosa, a south indian fermented food. | lactobacillus plantarum ld1 was isolated from dosa batter and identified by biochemical, physiological and genetic methods. species level identification was done by 16s rdna amplification and sequencing. the probiotic potential of strain ld1 was assessed by different standard parameters. cell surface hydrophobicity was recorded to be 62% with sat value <0.007 m. seventy-eight percent of viable count was found after treatment with simulated gastric juice containing pepsin (ph 2.0). bile salt tole ... | 2014 | 24676724 |
| functional characterization of a c-type lysozyme from indian shrimp fenneropenaeus indicus. | lysozyme gene from fenneropenaeus indicus was cloned, expressed in escherichia coli and characterized. the cdna consists of 477 base pairs and encodes amino acid sequence of 159 residues. f. indicus lysozyme had high identity (98%) with fenneropenaeus merguiensis and fenneropenaeus chinensis and exhibits low to moderate identities with lysozymes of other invertebrates and vertebrates. this lysozyme is presumed to be chicken types as it possesses two catalytic and eight cysteine residues that are ... | 2014 | 24676722 |
| selection of orphan rhs toxin expression in evolved salmonella enterica serovar typhimurium. | clonally derived bacterial populations exhibit significant genotypic and phenotypic diversity that contribute to fitness in rapidly changing environments. here, we show that serial passage of salmonella enterica serovar typhimurium lt2 (stlt2) in broth, or within a mouse host, results in selection of an evolved population that inhibits the growth of ancestral cells by direct contact. cells within each evolved population gain the ability to express and deploy a cryptic "orphan" toxin encoded with ... | 2014 | 24675981 |
| intestinal autophagy activity is essential for host defense against salmonella typhimurium infection in caenorhabditis elegans. | salmonella typhimurium infects both intestinal epithelial cells and macrophages. autophagy is a lysosomal degradation pathway that is present in all eukaryotes. autophagy has been reported to limit the salmonella replication in caenorhabditis elegans and in mammals. however, it is unknown whether intestinal autophagy activity plays a role in host defense against salmonella infection in c. elegans. in this study, we inhibited the autophagy gene bec-1 in different c. elegans tissues and examined t ... | 2014 | 24674884 |
| survival of foodborne pathogenic bacteria (bacillus cereus, escherichia coli o157:h7, salmonella enterica serovar typhimurium, staphylococcus aureus, and listeria monocytogenes) and bacillus cereus spores in fermented alcoholic beverages (beer and refined rice wine). | only limited information is available on the microbiological safety of fermented alcoholic beverages because it is still a common belief that such beverages do not provide a favorable environment for bacterial growth and survival. thus, in this study, we examined the survival of major foodborne pathogens and spores in fermented alcoholic beverages. foodborne pathogens (bacillus cereus, escherichia coli o157:h7, listeria monocytogenes, salmonella enterica serovar typhimurium, and staphylococcus a ... | 2014 | 24674433 |
| microbiological risk from minimally processed packaged salads in the dutch food chain. | the objective of this study was to evaluate the microbial hazard associated with the consumption of mixed salads produced under standard conditions. the presence of salmonella, campylobacter spp., and escherichia coli o157 in the dutch production chain of mixed salads was determined. microbial prevalence and concentration data from a microbiological surveillance study were used as inputs for the quantitative microbial risk assessment. chain logistics, production figures, and consumption patterns ... | 2014 | 24674430 |
| salmonella transfer during pilot plant scale washing and roller conveying of tomatoes. | salmonella transfer during washing and roller conveying of inoculated tomatoes was quantified using a pilot scale tomato packing line equipped with plastic, foam, or brush rollers. red round tomatoes (2.3 kg) were dip inoculated with salmonella enterica serovar typhimurium lt2 (avirulent) (4 log cfu/g), air dried for 2 h, and then washed in sanitizer-free water for 2 min. inoculated tomatoes were then passed single file over a 1.5-m conveyor equipped with plastic, foam, or brush rollers followed ... | 2014 | 24674428 |
| leveraging management strategies for seedborne plant diseases to reduce salmonella enterica serovar typhimurium incidence on tomato seed and seedlings. | tomatoes have been linked to many outbreaks of salmonellosis over the last decade, but the routes of contamination have yet to be discerned. many phytopathogens of tomato are seedborne and are effectively managed using seed sanitizers. seed sanitizers effective against bacterial phytopathogens were evaluated for their efficacy in killing bioluminescent salmonella enterica serovar typhimurium strain set-a14 on tomato seed infested with moderately high and high levels of pathogen. set-a14 incidenc ... | 2014 | 24674425 |
| characterization of the emerging salmonella 4,[5],12:i:- in danish animal production. | the monophasic salmonella variant with the antigenic formula salmonella 4,[5],12:i:- has emerged in the last decade as one of the main serotypes related to human salmonellosis. in the present study, a collection of 94 isolates of the s. 4,12:i:- and s. 4,5,12:i:- coming from danish farm animals, swine (86), cattle (7), and poultry (1), with well-defined identification was further typed by polymerase chain reaction serotyping, phage typing, and molecular typing (polymerase chain reaction and mult ... | 2014 | 24673107 |
| the mucosal inflammatory response to non-typhoidal salmonella in the intestine is blunted by il-10 during concurrent malaria parasite infection. | coinfection can markedly alter the response to a pathogen, thereby changing its clinical presentation. for example, non-typhoidal salmonella (nts) serotypes are associated with gastroenteritis in immunocompetent individuals. in contrast, individuals with severe pediatric malaria can develop bacteremic infections with nts, during which symptoms of gastroenteritis are commonly absent. here we report that, in both a ligated ileal loop model and a mouse colitis model, malaria parasites caused a glob ... | 2014 | 24670425 |
| proton-detected mas nmr experiments based on dipolar transfers for backbone assignment of highly deuterated proteins. | proton-detected solid-state nmr was applied to a highly deuterated insoluble, non-crystalline biological assembly, the salmonella typhimurium type iii secretion system (t3ss) needle. spectra of very high resolution and sensitivity were obtained at a low protonation level of 10-20% at exchangeable amide positions. we developed efficient experimental protocols for resonance assignment tailored for this system and the employed experimental conditions. using exclusively dipolar-based interspin magne ... | 2014 | 24667274 |
| molecular characterization of "inconsistent" variants of salmonella typhimurium isolated in italy. | salmonella 4,[5],12:i:- is a variant of salmonella typhimurium, which lacks the expression of phase-2 flagellar antigen, generally associated with the deletion of the fljb gene. additional mechanisms involving the fljab operon ( flja, fljb, and hin genes) lead to the lack of expression of phase-2 flagellar antigens also in salmonella strains harboring the fljb gene. for 20 s. 4,[5],12:i:- strains, defined as "inconsistent" salmonella typhimurium variants since they had phenotypically behaved as ... | 2014 | 24666380 |
| the primary transcriptome of salmonella enterica serovar typhimurium and its dependence on ppgpp during late stationary phase. | we have used differential rna-seq (drna-seq) to characterise the transcriptomic architecture of s. typhimurium sl1344, and its dependence on the bacterial alarmone, guanosine tetraphosphate (ppgpp) during late stationary phase, (lsp). under lsp conditions we were able to identify the transcriptional start sites (tsss) for 53% of the s. typhimurium open reading frames (orfs) and discovered 282 candidate non-coding rnas (ncrnas). the mapping of lsp tsss enabled a detailed comparison with a previou ... | 2014 | 24664308 |
| inverse agonist of estrogen-related receptor γ controls salmonella typhimurium infection by modulating host iron homeostasis. | in response to microbial infection, expression of the defensin-like peptide hepcidin (encoded by hamp) is induced in hepatocytes to decrease iron release from macrophages. to elucidate the mechanism by which salmonella enterica var. typhimurium (s. typhimurium), an intramacrophage bacterium, alters host iron metabolism for its own survival, we examined the role of nuclear receptor family members belonging to the nr3b subfamily in mouse hepatocytes. here, we report that estrogen-related receptor ... | 2014 | 24658075 |
| periplasmic disulfide isomerase dsbc is involved in the reduction of copper binding protein cuep from salmonella enterica serovar typhimurium. | salmonella enterica serovar typhimurium (s. typhimurium) is a facultative intracellular pathogen with the ability to survive and replicate in macrophages. periplasmic copper binding protein cuep is known to confer copper resistance to s. typhimurium, and has been implicated in ros scavenge activity by transferring the copper ion to a periplasmic superoxide dismutase or by directly reducing the copper ion. structural and biochemical studies on cuep showed that its copper binding site is surrounde ... | 2014 | 24657263 |
| immunological evaluation of mannosylated chitosan nanoparticles based foot and mouth disease virus dna vaccine, pvac fmdv vp1-ompa in guinea pigs. | a dna vaccine for foot and mouth disease (fmd) based on mannosylated chitosan nanoparticles was evaluated in guinea pigs. the dna construct was comprised of fmd virus full length-vp1 gene and outer membrane protein a (omp a) gene of salmonella typhimurium as a toll-like receptor (tlr)-ligand in pvac vector. groups of guinea pigs immunized either intramuscularly or intra-nasally were evaluated for induction of virus neutralizing antibodies, th1(igg2) and th2 (igg1) responses, lymphocyte prolifera ... | 2014 | 24656961 |
| invasive salmonella enterica serotype typhimurium infections, democratic republic of the congo, 2007-2011. | infection with salmonella enterica serotype typhimurium sequence type (st) 313 is associated with high rates of drug resistance, bloodstream infections, and death. to determine whether st313 is dominant in the democratic republic of the congo, we studied 180 isolates collected during 2007-2011; 96% belonged to crispol type ct28, which is associated with st313. | 2014 | 24655438 |
| a study on antimicrobial, antioxidant and antimutagenic activities of elaeagnus angustifolia l. leaves. | [corrected] the aim of this work was to investigate the antimicrobial, antioxidant, and antimutagenic potentials of methanol extracts from e. angustifolia. | 2014 | 24653563 |
| inhibition of listeria monocytogenes and salmonella by combinations of oriental mustard, malic acid, and edta. | the antimicrobial activities of oriental mustard extract alone or combined with malic acid and edta were investigated against salmonella spp. or listeria monocytogenes at different temperatures. five strain salmonella or l. monocytogenes cocktails were separately inoculated in brain heart infusion broth containing 0.5% (w/v) aqueous oriental mustard extract and incubated at 4 °c to 21 °c for 21 d. for inhibitor combination tests, salmonella typhimurium 02:8423 and l. monocytogenes 2-243 were ind ... | 2014 | 24649979 |
| a new fusion protein platform for quantitatively measuring activity of multiple proteases. | recombinant proteins fused with specific cleavage sequences are widely used as substrate for quantitatively analyzing the activity of proteases. here we propose a new fusion platform for multiple proteases, by using diaminopropionate ammonia-lyase (dal) as the fusion protein. it was based on the finding that a fused his6-tag could significantly decreases the activities of dal from e. coli (edal) and salmonella typhimurium (sdal). previously, we have shown that his6gst-tagged edal could be used t ... | 2014 | 24649897 |
| systemic administration of attenuated salmonella typhimurium in combination with interleukin-21 for cancer therapy. | attenuated salmonella typhimurium (s. typhimurium) strain vnp20009 has been employed as a powerful anticancer agent due to its selective accumulation in tumors for targeted therapy. s. typhimurium has been demonstrated to constitute a delivery tool carrying antiangiogenic or proapoptotic genes that treat cancer. the hydrodynamic tail vein (htv) injection of naked plasmid dna has been developed as an effective gene delivery strategy, which has been successfully used in vivo. the aim of this study ... | 2013 | 24649192 |
| salmonella enterica serovar typhi impairs cd4 t cell responses by reducing antigen availability. | salmonella enterica serovar typhi is associated with a disseminated febrile illness in humans, termed typhoid fever, while salmonella enterica serovar typhimurium causes localized gastroenteritis in immunocompetent individuals. one of the genetic differences between both pathogens is the presence in s. typhi of tvia, a regulatory protein that shuts down flagellin (flic) expression when bacteria transit from the intestinal lumen into the intestinal mucosa. here we investigated the consequences of ... | 2014 | 24643532 |
| association of nuisance filamentous algae cladophora spp. with e. coli and salmonella in public beach waters: impacts of uv protection on bacterial survival. | this study investigated whether filamentous algal species commonly found in nearshore public beach water systems provide protection from natural uv to bacteria present in the same environmental settings. to test this hypothesis, cladophora spp., a filamentous nuisance algae group causing undesired water quality in the great lakes region was selected and its interactions with a non-pathogenic indicator organism escherichia coli and a pathogenic strain of salmonella enterica serovar typhimurium we ... | 2014 | 24643370 |
| pre- and post-processing workflow for affinity purification mass spectrometry data. | the reliable detection of protein-protein interactions by affinity purification mass spectrometry (ap-ms) is crucial for the understanding of biological processes. quantitative information can be used to separate truly interacting proteins from false-positives by contrasting counts of proteins binding to specific baits with counts of negative controls. several approaches have been proposed for computing scores for potential interaction proteins, for example, the commonly used saint software. how ... | 2014 | 24641689 |
| structure of bacteriophage spn1s endolysin reveals an unusual two-module fold for the peptidoglycan lytic and binding activity. | bacteriophage spn1s infects the pathogenic gram-negative bacterium salmonella typhimurium and expresses endolysin for the release of phage progeny by degrading peptidoglycan of the host cell walls. bacteriophage spn1s endolysin exhibits high glycosidase activity against peptidoglycans, resulting in antimicrobial activity against a broad range of outer membrane-permeabilized gram-negative bacteria. here, we report a crystal structure of spn1s endolysin, indicating that unlike most endolysins from ... | 2014 | 24641441 |
| oxidative metabolism enables salmonella evasion of the nlrp3 inflammasome. | microbial infection triggers assembly of inflammasome complexes that promote caspase-1-dependent antimicrobial responses. inflammasome assembly is mediated by members of the nucleotide binding domain leucine-rich repeat (nlr) protein family that respond to cytosolic bacterial products or disruption of cellular processes. flagellin injected into host cells by invading salmonella induces inflammasome activation through nlrc4, whereas nlrp3 is required for inflammasome activation in response to mul ... | 2014 | 24638169 |
| oral administration with attenuated salmonella encoding a trichinella cystatin-like protein elicited host immunity. | trichinellosis is a public health problem and is regarded as an emergent/re-emergent disease in various countries. the cdna encoding a cystatin-like protein (ts-cystatin) was identified by immunoscreening intestinal muscle larvae cdna libraries with serum from pigs experimentally infected with 20,000 trichinella spiralis muscle larvae. to study its impact on host immunity, we chose a eukaryotic expression system based on several comparisons of immunogenicity between the two salmonella typhimuriu ... | 2014 | 24636891 |
| nisin/β-lactam adjunct therapy against salmonella enterica serovar typhimurium: a mechanistic approach. | multidrug resistance exhibited by salmonella strains has proved to be a big hurdle in the development of an effective anti-salmonella therapy. in this context, we had previously demonstrated strong synergism of nisin/ceftriaxone and nisin/cefotaxime combinations against salmonella enterica serovar typhimurium. however, the mechanism remained unexplored. the present study was therefore planned in order to evaluate the underlying mechanisms responsible for the synergistic effect of nisin in combin ... | 2014 | 24633205 |
| salmonella enterica serovar typhimurium-infected pigs with different shedding levels exhibit distinct clinical, peripheral cytokine and transcriptomic immune response phenotypes. | foodborne salmonellosis costs the us $2.7 billion/year, including $100.0 million in annual losses to pork producers. pigs colonized with salmonella are usually asymptomatic with varied severity and duration of fecal shedding. thus, understanding the responses that result in less shedding may provide a mechanism for control. fifty-four pigs were inoculated with salmonella enterica serovar typhimurium (st) and clinical signs, fecal st shedding, growth performance, peripheral cytokines and whole bl ... | 2015 | 24632525 |