Publications

selection and reversal of plasmodium berghei resistance in the mouse model following repeated high doses of artemether.	artemether, a derivative of artemisinin, is effectively used for the treatment of malaria without any clinically relevant resistance to date. artemether has also been developed as an antischistosomal agent, exhibiting highest activity against immature parasites. here, we employ a rodent model and investigate whether the proposed artemether treatment schedule to prevent schistosome-attributable morbidity might select for plasmodium berghei resistance. mice infected with an anka strain of p. bergh ...	2004	14677058
activation of transforming growth factor beta by malaria parasite-derived metalloproteinases and a thrombospondin-like molecule.	much of the pathology of malaria is mediated by inflammatory cytokines (such as interleukin 12, interferon gamma, and tumor necrosis factor alpha), which are part of the immune response that kills the parasite. the antiinflammatory cytokine transforming growth factor (tgf)-beta plays a crucial role in preventing the severe pathology of malaria in mice and tgf-beta production is associated with reduced risk of clinical malaria in humans. here we show that serum-free preparations of plasmodium fal ...	2003	14676296
combination effects of chloroquine with the febrifugine and isofebrifugine mixture against a blood-induced infection with chloroquine-resistant plasmodium berghei nk65 in icr mice.	the combination effects of chloroquine with a mixture of febrifugine and isofebrifugine were evaluated against a blood-induced infection with chloroquine-resistant p. berghei nk65 in icr mice. mice in the untreated control showed a progressively increasing parasitemia leading to mouse death. a two-day dosage of 20 mg base/kg of chloroquine alone showed little effect against p. berghei nk65 infection, and all mice died from day 13 to 14 with an increasing parasitemia. a four-day dosage of 1 mg/kg ...	2003	14669265
the plasmodium circumsporozoite protein is involved in mosquito salivary gland invasion by sporozoites.	plasmodium sporozoites develop in oocysts on the midgut wall of the mosquito and are released into the hemocoel. approximately 15-20% of oocyst sporozoites will successfully attach to and invade salivary glands, their target organ. we have previously shown that the major surface protein of sporozoites, the circumsporozoite (cs) protein, binds specifically to salivary glands and not to other mosquito organs exposed to circulating hemolymph. in addition, a peptide from the n-terminal portion of cs ...	2004	14668012
malaria parasites lacking eef1a have a normal s/m phase yet grow more slowly due to a longer g1 phase.	eukaryotic elongation factor 1a (eef1a) plays a central role in protein synthesis, cell growth and morphology. malaria parasites possess two identical genes encoding eef1a (eef1aa and eef1ab). using pbeef1a-plasmodium berghei mutants that lack an eef1a gene, we demonstrate that the level of eef1a production affects the proliferation of blood stages and parasite fitness. pbeef1a- parasites can complete the vertebrate and mosquito phases of the life cycle, but the growth phase of the asexual blood ...	2003	14651637
analysis of the plasmodium and anopheles transcriptional repertoire during ookinete development and midgut invasion.	plasmodium, the causative agent of malaria, has to undergo sexual differentiation and development in anopheline mosquitoes for transmission to occur. to isolate genes specifically induced in both organisms during the early stages of plasmodium differentiation in the mosquito, two cdna libraries were constructed, one enriched for sequences expressed in differentiating plasmodium berghei ookinetes and another enriched for sequences expressed in anopheles stephensi guts containing invading ookinete ...	2004	14627712
analysis of the plasmodium and anopheles transcriptomes during oocyst differentiation.	understanding the life cycle of the malaria parasite in its mosquito vector is essential for developing new strategies to combat this disease. subtractive hybridization cdna libraries were constructed that are enriched for plasmodium berghei and anopheles stephensi genes expressed during oocyst differentiation on the midgut. sequencing of 1485 random clones led to the identification of 1137 unique expressed sequence tags. of the 608 expressed sequence tags with data base hits, 320 (53%) had sign ...	2004	14627711
the role of reactive oxygen species on plasmodium melanotic encapsulation in anopheles gambiae.	malaria transmission depends on the competence of some anopheles mosquitoes to sustain plasmodium development (susceptibility). a genetically selected refractory strain of anopheles gambiae blocks plasmodium development, melanizing, and encapsulating the parasite in a reaction that begins with tyrosine oxidation, and involves three quantitative trait loci. morphological and microarray mrna expression analysis suggest that the refractory and susceptible strains have broad physiological difference ...	2003	14623973
in-vivo antimalarial activity of some oxygenated xanthones.	a series of oxygenated xanthones was prepared so that the antimalarial activity of each compound could be evaluated in vivo, using 4-day suppressive assays against plasmodium berghei anka in balb/c mice. when given in a dose of 20 mg/kg.day for 4 days, most of the compounds produced significant chemosuppression of parasitaemia. the most active compound was 1,3,6,8-tetrahydroxyxanthone, which reduced the percentage of erythrocytes infected by 70.5%, followed by norlichexanthone (44.3%) and its is ...	2003	14613627
isolation of plasmodium berghei ookinetes in culture using nycodenz density gradient columns and magnetic isolation.	background: large scale in vitro production of the mosquito stages of malaria parasites remains elusive, with only limited success for complete sporogonic development and only one report of development through to infective sporozoites. the initial step in this process is the production, in vitro, of ookinetes from gametocytaemic blood. methods for isolation of these ookinetes from blood cells have been described; however, in addition to yield often being low, processing time and potential for co ...	2003	14613512
protection of mice infected with plasmodium berghei by bacillus thuringiensis crystal proteins.	eight bacillus thuringiensis strains were used to test their activity against plasmodium berghei. when crystal proteins extracted from strains 007, 017, 020, 021, 030, 032, and 037 were injected into plasmodium-infected mice through the tail vein at a rate of 0.45-1.5 mg per mouse, the lengths of survival for the mice were extended up to 5 days (from 8.5 days to 13.5-15 days). blood-cell staining demonstrated that normal erythrocytes were lightly stained and regularly shaped while the erythrocyt ...	2004	14600831
baculovirus virions displaying plasmodium berghei circumsporozoite protein protect mice against malaria sporozoite infection.	the display of foreign proteins on the surface of baculovirus virions has provided a tool for the analysis of protein-protein interactions and for cell-specific targeting in gene transfer applications. to evaluate the baculovirus display system as a vaccine vehicle, we have generated a recombinant baculovirus (acnpv-cspsurf) that displays rodent malaria plasmodium berghei circumsporozoite protein (pbcsp) on the virion surface as a fusion protein with the major baculovirus envelope glycoprotein g ...	2003	14599800
inhibition of platelet adherence to brain microvasculature protects against severe plasmodium berghei malaria.	some patients with plasmodium falciparum infections develop cerebral malaria, acute respiratory distress, and shock and ultimately die even though drug therapy has eliminated the parasite from the blood, suggesting that a systemic inflammatory response contributes to malarial pathogenesis. plasmodium berghei-infected mice are a well-recognized model of severe malaria (experimental severe malaria [esm]), and infected mice exhibit a systemic inflammatory response. because platelets are proposed to ...	2003	14573677
identification and expression analysis of abc genes in plasmodium yoelii and p. berghei.	the atp-binding cassette (abc) proteins are one of the largest evolutionarily conserved families. they are characterized by the presence of highly conserved nucleotide-binding sites (nbs). in the present study, we identified abc genes in rodent plasmodia. we queried the plasmodium yoelii genome with the abc signature motif and retrieved 15 contigs. sequences were classified into seven abc families by blast comparison. conservation of the five signature abc motifs in the p. yoelii contigs was exa ...	2004	14564508
[genetic analysis of host resistance to rodent malaria in mice].		2003	14562624
heterologous promoter activity in stable and transient plasmodium knowlesi transgenes.		2003	14550898
plasmepsin 4, the food vacuole aspartic proteinase found in all plasmodium spp. infecting man.	plasmepsins are aspartic proteinases of the malaria parasite, and seven groups of plasmepsins have been identified by comparing genomic sequence data available for the genes encoding these enzymes from plasmodium falciparum, plasmodium vivax, plasmodium knowlesi, plasmodium berghei, and plasmodium yoelii. the food vacuole plasmepsins typified by plasmepsin 4 from p. falciparum (pfpm4) constitute one of these groups. genes encoding the ortholog of pfpm4 have been cloned from plasmodium ovale, pla ...	2003	14550891
rheumatoid factor-like igm in plasmodium berghei (apicomplexa: haemosporida) infections of balb/c mice.	groups of female balb/c mice infected by intravenous injection with 50 erythrocytes containing plasmodium berghei vincke et lips, 1948 were sacrificed on days 3 through 12 after infection. rheumatoid factor-like igm (rf-igm) and parasite-specific igg levels were determined by enzyme-linked immunosorbent assay in serum specimens and in culture medium removed from spleen cell cultures established at sacrifice. all four mouse igg subisotypes were recognized by rf-igm molecules induced by plasmodium ...	2003	14535342
8-quinolinamines and their pro prodrug conjugates as potent blood-schizontocidal antimalarial agents.	synthesis and antimalarial activities of n8-(4-amino-1-methylbutyl)-5-alkoxy-4-ethyl-6-methoxy-8-quinolinamines (5) and their pro prodrug analogues (6-7) prepared by covalently linking 5 to the redox-sensitive (8) and esterase-sensitive (9) linkers through the amide linkage are reported. the most effective 8-quinolinamines [5c (r=c5h11) and 5f (r=c8h17)] have exhibited in vitro and in vivo biological efficacy superior to that of the standard drug chloroquine against both drug-sensitive and drug- ...	2003	14527552
rodent malaria in the natural host--irradiated sporozoites of plasmodium berghei induce liver-stage specific immune responses in the natural host grammomys surdaster and protect immunized grammomys against p. berghei sporozoite challenge.	the choice of the host in studying host-parasite interactions is of crucial importance, and the use of a natural host is most appropriate in answering pertinent questions related to human malaria. the grammomys surdaster is the natural host and reservoir of the rodent malaria parasite plasmodium berghei. this natural host is difficult to protect by irradiated sporozoite immunization, a situation comparable to what has been observed in humans with p. falciparum. this is in contrast to the complet ...	2001	14513935
quinine distribution in mice with plasmodium berghei malaria.	the disposition of a single 80 mg/kg injection of quinine base was compared in control and plasmodium berghei-infected mice. pharmacokinetic parameters were determined on repeated whole blood samples from caudal vein (experiment 1) and quinine distribution was evaluated in tissues and blood fractions from mice sacrificed two hours post dosing (experiment 2). quinine concentrations were assessed by high performance liquid chromatography with fluorometric detection. whole blood concentrations and ...	2003	14503660
erythrocyte g protein-coupled receptor signaling in malarial infection.	erythrocytic mechanisms involved in malarial infection are poorly understood. we have found that signaling via the erythrocyte beta2-adrenergic receptor and heterotrimeric guanine nucleotide-binding protein (galphas) regulated the entry of the human malaria parasite plasmodium falciparum. agonists that stimulate cyclic adenosine 3',5'-monophosphate production led to an increase in malarial infection that could be blocked by specific receptor antagonists. moreover, peptides designed to inhibit ga ...	2003	14500986
why is the plasmodium falciparum hexose transporter a promising new drug target?	chemotherapy of malaria parasites is limited by established drug resistance and lack of novel treatment options. intraerythrocytic stages of plasmodium falciparum, the causative agent of severe malaria, are wholly dependent upon host glucose for energy. a facilitative hexose transporter (pfht), encoded by a single-copy gene, mediates glucose uptake and is therefore an attractive potential target. the authors first established heterologous expression in xenopus laevis to allow functional characte ...	2003	14498822
studies on plasmodium berghei vincke and lips, 1948. xxix. the size of parasite population and its relation to the selection of a strain resistant to sulphadiazine.		1961	14490070
studies on plasmodium berghei vincke and lips, 1948. xxx. effects of splenectomy on the course of blood-induced infection in rats.		1961	14488473
studies on plasmodium berghei vincke and lips, 1948. xxxi. selection of a primaquine resistant strain.		1961	14488472
metastatic calcification induced by hytakerol in rats infected with plasmodium berghei.		1962	14472743
experimental lysin-deficiency in rats infected with plasmodium berghei.		1962	14467528
[the course of infection and clinical picture of plasmodium berghei-infected nmri strain mice].		1961	14459600
[resistance and immunity in plasmodium berghei infected mice].		1962	14459599
the behaviour of plasmodium berghei and plasmodium vinckei in the spiny mouse acomys cahirinus.		1961	14448536
the effect of anti-erythrocytic antibodies upon plasmodium berghei infections in white mice.		1960	14444152
[influence of vitamins b 12, c and k 1 in the treatment of plasmodium berghei infections using chloroquine].		1959	14441980
[studies on the experimental production of resistance to various antimalarial products in a strain of plasmodium berghei].		1959	14441979
[reappearance of the process of extraflagellation in a strain of plasmodium berghei regularly maintained by mechanical passage].		1959	14406483
[effect of various diets on the development of plasmodium berghei in the white mouse].		1955	14393134
pathological and immunological host-parasite relationships between albino rat and plasmodium berghei.		1954	14391893
some observations about immunity to plasmodium berghei.		1954	14391892
the absence of cross immunity between plasmodium berghei (vincke and lips) and plasmodium vinckei (rodhain).		1954	14391891
investigations on immunity to plasmodium berghei infection in mice.		1954	14391890
immunology of plasmodium berghei.		1954	14391889
experimental study on the immunology of malaria due to plasmodium berghei.		1954	14391888
malaria and nutrition with special reference to plasmodium berghei infections in rats.		1954	14391887
some host-parasite relationships in plasmodium berghei infections.		1954	14391886
studies on plasmodium berghei vincke and lips, 1948. xx. a physiological change observed in sulphadiazine resistant strain.		1954	14391884
on some problems arising from the observation of the infection with plasmodium berghei in mice and rats.		1954	14391883
some physiological and pathological processes in plasmodium berghei infections in white rats.		1954	14391882
plasmodium berghei and chemotherapy.		1954	14391881
the mosquito transmission of plasmodium berghei.		1954	14391880
experimental transmission of plasmodium berghei.		1954	14391879
natural history of plasmodium berghei.		1954	14391878
the history of the discovery of plasmodium berghei.		1954	14391877
a written symposium on plasmodium berghei vincke and lips, 1948; introduction.		1954	14391876
symposium on plasmodium berghei.		1954	14391875
[development of plasmodium berghei infection in newborn rats].		1954	14378933
[development of plasmodium berghei infection in mice in the midi (france)].		1954	14378917
[research on plasmodium berghei (vincke and lips) infection in laboratory mice. ii. chemotherapeutic trials].		1955	14377160
[plasmodium berghei infections in milk-fed splenectomized rats].		1954	14372842
[some observations with reference to immunity to plasmodium berghei].		1954	14362172
[research on plasmodium berghei malaria in laboratory mice. i. some factors with a possible influence on the severity of the infection].		1955	14362142
[absence of cross immunity between plasmodium berghei and plasmodium vinckei in infections in young rats].		1954	14352552
[experimental immunological study of paludism with plasmodium berghei].		1954	14350825
[appearance of immunity against plasmodium berghei in mice subjected to milk diet or to sulfonamide therapy].		1955	14350363
electron microscopy of plasmodium berghei. i. on the migration of trophozoites from infected erythrocytes in the rat.		1965	14347459
[studies on the effect of the infection dose on the survival time in plasmodium berghei infection in mice].		1965	14343725
[studies on the importance of complement binding antibodies for plasmodium berghei infection in mice].		1965	14343724
[dietetic influence on malaria (plasmodium berghei) in the mouse].		1964	14340331
[on the pathogenesis of anemia in (plasmodium berghei) malarial infection in nmri mice].		1964	14309963
studies on plasmodium berghei in nature and under experimental conditions.		1965	14298028
phagotrophy and pigment formation in a chloroquine-resistant strain of plasmodium berghei vincke and lips, 1948.		1965	14297348
pre-erythrocytic development of plasmodium berghei.		1965	14287427
competitive relationship between eperythrozoon coccoides and plasmodium berghei in the mouse.		1965	14281212
quinine-resistant plasmodium berghei in mice.	during induction of chloroquine resistance, plasmodium berghei developed resistance to quinine administered in doses near the maximum amounts tolerated by mice. resistant parasites did not form malarial pigment. normal sensitivity to both quinine and chloroquine returned and pigment formation resumed during serial passage of the parasites through untreated mice.	1965	14280010
studies on a dihydrotriazine and a sulfone, alone and in combination, against plasmodium berghei in mice.		1965	14270445
immunofluorescent studies of plasmodium berghei: a "natural" antibody in white mice.		1965	14270443
selection of a strain of albino mice refractory to plasmodium berghei infection.	some features of the natural history of malaria in holoendemic areas can be explained by the presence of acquired immunity. there is also evidence that transmission of humoral antibodies in utero from the immune mother plays some part in the transient protection of the offspring. little is known about the importance of a genetic factor.the possibility of inheritance of immunity to malaria was investigated on six generations of a strain of white mice originating from 50 parents fully susceptible ...	1964	14267748
pyruvate and lactate levels in relationship to the nicotinamide--adenine dinucleotide levels in malarial parasites (plasmodium berghei).		1964	14249147
[erythro- and lymphopoiesis in the mouse spleen in plasmodium berghei infection].		1964	14247127
pigment formation and nuclear division in chloroquine-resistant malaria parasites (plasmodium berghei, vincke and lips, 1948).		1964	14230214
electron microscopic observations of plasmodium berghei and the kupffer cell in the liver of rats.		1964	14215478
a study of plasmodium berghei in thamnomys surdaster, and in other experimental hosts.		1964	14205883
role of p-aminobenzoic acid in plasmodium berghei infection in the mouse.		1964	14191322
the effect of different sera in the culture medium on the behavior of plasmodium berghei following serial passage through tissue culture.		1964	14179745
plasmodium berghei: cyclical transmissions by experimentally infected anopheles quadrimaculatus.	a number of strains of plasmodium berghei were isolated from sporozoites of anopheles dureni. laboratory-bred anopheles quadrimaculatus fed on carriers of the newly isolated strains showed overwhelming midgut infections and moderate or mild salivary gland infections. successive cyclic transmissions by the bite of experimentally infected a. quadrimaculatus in laboratorybred tree rats (thamnomys surdaster) were carried out.	1964	14169345
[blood cytochemical studies in experimental infection of white mice with plasmodium berghei. i. cytochemical structure of the parasite, of the erythrocytes, and observations carried out with the phase contrast microscope].		1963	14165822
a study on the mechanism of anaemia in white rats infected with plasmodium berghei.		1964	14153134
movement of the sporozoites of plasmodium berghei (vincke et lips, 1948).		1964	14151438
modification of a malaria parasite (plasmodium berghei) following passage through tissue culture.		1964	14134736
measurements of the acquired resistance of rats and mice to plasmodium berghei infections.		1964	14125164
[histopathological changes in the albino rat infected with plasmodium berghei. iii. observations made 2 weeks, 1 month and 3 months after the parasitolytic crisis].		1963	14123072
the measurement of antibody in human malaria by a formolized tanned sheep cell haemagglutination test.	a major obstacle to serological testing for malaria immunity has been the difficulty in obtaining sufficient antigen from the small, intracellular parasite. this is overcome by the formolized, tanned, sheep red cell haemagglutination test described in this paper, as only minute quantities of antigen protein and very small amounts of patient's serum are required for its performance.in the trials reported, antigens derived from plasmodium berghei, p. vivax, p. coatneyi and p. cynomolgi were tested ...	1964	14122439
detection of a plasmodium berghei-antibody complex formed in vivo.		1964	14106057
[the spleen and malaria. the course of infection (plasmodium berghei) in splenectomized nmri mice and its significance based on histopathological changes in the spleen of nonsplenectomized mice].		1963	14093657
[histopathological changes in the albino rat infected with plasmodium berghei. ii. observations at the moment of parasystolic crisis and in the days immediately after].		1963	14088552
gametogony and sporogony in a strain of plasmodium berghei preserved at low temperature.		1963	14084197
an evaluation of antimalarial combinations against plasmodium berghei in the mouse.		1963	14084196
cortisone and antimalarial drug activity against plasmodium berghei.		1963	14064151
[contribution to the study of the changes in the ground substance in experimental infestation with plasmodium berghei in mice].		1963	14053493
elimination of plasmodium berghei by way of the respiratory tract in mice.		1963	14047024
penetration of a mouse erythrocyte by a merozoite of plasmodium berghei as revealed by electron microscopy.		1963	14047023