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effects of tea polyphenols on emulsification of olive oil in a small intestine model system.tea catechins have been shown to reduce plasma cholesterol and suppress hypertriacylglycerolemia by reducing triglyceride absorption. however, the mechanism is not yet clear. one of the possible mechanisms is that tea polyphenols may modify dietary fat emulsification in the gastrointestinal tract. the digestive enzyme (lipase) acts on specific emulsion interface properties (droplet size and surface area). therefore, changes in these properties may modify emulsification and lead to changes in die ...200616506852
chronic black tea administration protects plasma proteins, plasma, liver and kidney lipids against oxidation.black tea is known to have protective effects against plasma lipid and lipoprotein oxidation, but its influence on lipid peroxidation in tissue has been less studied. the effect of oral black tea consumption on protein oxidation has also not been demonstrated. the present study investigated the antioxidant effects of oral black tea consumption.200616501415
triterpene saponins with gastroprotective effects from tea seed (the seeds of camellia sinensis).six new triterpene saponins, theasaponins a(1) (1), a(2) (2), a(3) (3), f(1) (4), f(2) (5), and f(3) (6), were isolated from the saponin fraction of the seeds of camellia sinensis. the stereostructures of 1-6 were elucidated on the basis of chemical and physicochemical evidence. theasaponin a(2) (2) showed an inhibitory effect on ethanol-induced gastric mucosal lesions in rats at a dose of 5.0 mg/kg, p.o., and its activity was more potent than that of omeplazole. structure-activity relationships ...200616499314
antimicrobial activities of tea catechins and theaflavins and tea extracts against bacillus cereus.we evaluated the antimicrobial activities of seven green tea catechins and four black tea theaflavins, generally referred to as flavonoids, as well as the aqueous extracts (infusions) of 36 commercial black, green, oolong, white, and herbal teas against bacillus cereus (strain rm3190) incubated at 21 degrees c for 3, 15, 30, and 60 min. the results obtained demonstrate that (i) (-)-gallocatechin-3-gallate, (-)-epigallocatechin-3-gallate, (-)-catechin-3-gallate, (-)-epicatechin-3-gallate, theafla ...200616496576
targeting angiogenesis with integrative cancer therapies.an integrative approach for managing a patient with cancer should target the multiple biochemical and physiological pathways that support tumor development while minimizing normal tissue toxicity. angiogenesis is a key process in the promotion of cancer. many natural health products that inhibit angiogenesis also manifest other anticancer activities. the authors will focus on natural health products (nhps) that have a high degree of antiangiogenic activity but also describe some of their many ot ...200616484711
neuroprotective and neurorescue effect of black tea extract in 6-hydroxydopamine-lesioned rat model of parkinson's disease.in the present study, an attempt has been made to explore the neuroprotective and neuroreparative (neurorescue) effect of black tea extract (bte) in 6-hydroxydopamine (6-ohda)-lesioned rat model of parkinson's disease (pd). in the neuroprotective (bte + 6-ohda) and neurorescue (6-ohda + bte) experiments, the rats were given 1.5% bte orally prior to and after intrastriatal 6-ohda lesion respectively. a significant recovery in d-amphetamine induced circling behavior (stereotypy), spontaneous locom ...200616480889
5s rdna gene diversity in tea (camellia sinensis (l.) o. kuntze) and its use for variety identification.variability in the organization of repeats of 5s rdna is useful for phylogenetic studies in various crops. we found variable repeats of 5s rdna gene in the genome of tea (camellia sinensis (l.) o. kuntze) during southern hybridization. variability in the repeats of 5s rdna with specific restriction endonucleases (sau3ai, bamhi, and apoi) was analyzed in 28 different tea clones representing 3 types of tea. our results clearly show that the 5s rdna gene in tea could be used as a molecular marker t ...200616462906
clinical studies to determine the effectiveness of a whitening toothpaste at reducing stain (using a forced stain model).two single centre, randomized single-blind, crossover studies were performed, to compare the effect of a test toothpaste with a conventional fluoride paste in the inhibition and removal of extrinsic dental stain promoted by repeated chlorhexidine/tea rinses.200516451374
characterization of the key aroma compounds in the beverage prepared from darjeeling black tea: quantitative differences between tea leaves and infusion.by application of the aroma extract dilution analysis on the volatile fraction isolated from a black tea infusion (darjeeling gold selection), vanillin (vanilla-like), 4-hydroxy-2,5-dimethyl-3(2h)-furanone (caramel), 2-phenylethanol (flowery), and (e,e,z)-2,4,6-nonatrienal (oat-flake-like) were identified with the highest flavor dilution (fd) factors among the 24 odor-active compounds detected in the fd factor range of 4-128. quantitative measurements performed by means of stable isotope dilutio ...200616448203
green tea (camellia sinensis) extract ameliorates endotoxin induced sickness behavior and liver damage in rats.immune activation, either by cytokines or endotoxin, elicits a constellation of nonspecific symptoms such as weakness, malaise, listlessness, fatigue, adipsia, anorexia, depression and anxiety collectively termed as sickness behavior. further, endotoxin administration in animals has been implicated in the pathogenesis of many types of liver disease. green tea, a common household drink, is rich in antioxidant polyphenols demonstrating inhibitory effects on cytokine production. the present study w ...200616444665
bactericidal and anti-adhesive properties of culinary and medicinal plants against helicobacter pylori.to investigate the bactericidal and anti-adhesive properties of 25 plants against helicobacter pylori (h. pylori).200516437723
tumor-induced thymic involution via inhibition of il-7r alpha and its jak-stat signaling pathway: protection by black tea.down-regulation of cell-mediated immune functions occurring at late stages of cancer may be related to the thymic involution since thymus is the major site of t cell maturation, proliferation, and differentiation. we found that in ehrlich's ascites carcinoma (eac)-bearing mice there was profound depletion of cd4+ and cd8+ cells in peripheral blood with severely damaged thymus on 21st day of tumor inoculation. however, treatment with black tea at an antitumor dose of 2.5% significantly reduced su ...200616428079
chemoprevention of lung cancer by tea.tea is the second only to water as the most consumed beverage in the world. both green and black teas have been studied for their health benefits for a variety of diseases, particularly cancer. lung cancer is the predominant cause of cancer mortality in developed countries. smokers' risk of lung cancer is 20 times that of persons who have never smoked. epidemiological studies on the cancer-preventive effects of tea produce inconsistent results, which could in part be attributed to the lack of a ...200616425282
short-term bioaccumulation of vanadium when ingested with a tea decoction in streptozotocin-induced diabetic rats.sodium orthovanadate suspended in a lichee black tea decoction effectively regulates blood glucose levels in rats with insulin-dependent, streptozotocin (stz)-induced diabetes. the primary advantage of vanadate delivery with the tea decoction over conventional systems that use water suspensions of vanadate is a significant reduction in the toxic side effects of vanadate. it is unknown if the tea alters the bioavailability of vanadate. male sprague-dawley rats were administered an intravenous inj ...200616423636
neuroprotective effects of green and black teas and their catechin gallate esters against beta-amyloid-induced toxicity.teas represent a large family of plants containing high amounts of polyphenols that may confer health benefits in various diseases. recently, it has been hypothesized that tea consumption may also reduce the risk of age-related neurodegenerative pathologies. considering the deleterious role of beta-amyloid (abeta) in the aetiology of alzheimer's disease (ad), we investigated green and black tea extracts and flavan-3-ols (present as monomers and dimers in green and black forms, respectively) agai ...200616420415
the role of tea and tea flavonoids in cardiovascular health.consumption of green or black tea has been inversely associated with the development and progression of cardiovascular diseases. in this review, the current knowledge about protective effects of tea and tea constituents, particularly flavonoids, on the cardiovascular system is summarized. underlying mechanisms for the beneficial effects of tea include vasculoprotective, antioxidative, antithrombogenic, anti-inflammatory, and lipid-lowering properties of tea flavonoids. although promising experim ...200616404706
inhibitory effects of black tea theaflavin derivatives on 12-o-tetradecanoylphorbol-13-acetate-induced inflammation and arachidonic acid metabolism in mouse ears.tea has been shown to possess several health beneficial properties primarily due to its polyphenolic content. the major polyphenolic compounds in black tea leaves are theaflavins (tfs) formed by oxidative coupling of catechins in tea leaves during its processing. in this paper, we report the characterization of 12-o-tetradecanoylphorbol-13-acetate (tpa)-induced mouse ear inflammatory model and the inhibitory effects of major black tea tfs derivatives on this inflammation. in addition, the effect ...200616404705
maximum residue limit and risk assessment of spiromesifen (bay bsn 2060; oberon 240sc) on tea (camellia sinensis (l) o' kuntze). 200516400559
inhibition of angiotensin converting enzyme activity by flavanol-rich foods.angiotensin converting enzyme (ace) activity was evaluated in the presence of flavanol-rich foods, i.e., wines, chocolates, and teas, and of purified flavonoids. all foods assayed inhibited ace activity, red wines being more effective than white wine, and green tea more effective than black tea. the inhibition of ace activity was associated with both phenolic and flavanol content in the foods. when isolated polyphenols were assayed, procyanidins (dimer and hexamer) and epigallocatechin significa ...200616390204
catechin transformation as influenced by aluminum.polyphenols (catechins) are vital biomolecules in tea plants (camellia sinensis), which are well-known as typical al accumulators. however, the interaction between al and catechin remains obscured. the objective of the present study was to investigate the effect of al on the transformation of (+)-catechin. solutions with oh/al molar ratios of 2.5 (ph 5.5) and 3.0 (ph 7.0) prepared at al/catechin molar ratios (r) of 0, 0.2, 0.4, 0.6, 0.8, and 1.0 were aged for 7 and 30 days, respectively. the pre ...200616390201
hepatotoxicity associated with supplements containing chinese green tea (camellia sinensis). 200616389263
inhibition of cwr22rnu1 tumor growth and psa secretion in athymic nude mice by green and black teas.cancer of the prostate gland (cap), the most common invasive malignancy and a major cause of cancer related deaths in male population in the usa, is an ideal candidate disease for chemoprevention because it is typically detected in elderly population with a relatively slower rate of growth and progression. many dietary phytochemicals are showing promising chemopreventive effects, at-least in pre-clinical models of cap. our published data in cell culture and animal studies, supported by the work ...200616387739
green tea consumption in everyday life and mental health.green tea has been widely acknowledged in japan to induce a pleasurable mental feeling. recent laboratory studies have suggested positive psychological effects as a result of consuming green tea. the present study examined whether green tea consumption in everyday life in japan is associated with positive mental health.200516372926
the effect of an herbal supplement containing black tea and caffeine on metabolic parameters in humans.the objective of this study was to test an herbal supplement containing black tea (the fully oxidized form of camellia sinensis) and caffeine for stimulation of thermogenesis.200516366740
consumption of coffee, but not black tea, is associated with decreased risk of premenopausal breast cancer.caffeine has been suggested as a possible risk factor for breast cancer, potentially through its effect of facilitating the development of benign breast disease. however, coffee and tea also contain polyphenols, which exhibit anticarcinogenic properties. a hospital-based, case-control study was conducted to evaluate the role of coffee, decaffeinated coffee, and black tea in breast cancer etiology. study participants included 1932 cases with primary, incident breast cancer and 1895 hospital contr ...200616365077
of the major phenolic acids formed during human microbial fermentation of tea, citrus, and soy flavonoid supplements, only 3,4-dihydroxyphenylacetic acid has antiproliferative activity.dietary flavonoids are poorly absorbed from the gastrointestinal tract. colonic bacteria convert flavonoids into smaller phenolic acids (pa), which can be absorbed into the circulation and may contribute to the chemopreventive activity of the parent compounds. the purpose of our study was to determine whether flavonoids from green and black tea (gt, bt), citrus fruit with rutin (cf+r) and soy (s) supplements exposed to the same conditions in a dynamic in vitro model of the colon (tim-2) will for ...200616365058
safety studies on epigallocatechin gallate (egcg) preparations. part 1: genotoxicity.public interest in green tea has grown recently due to the potential health benefits from its consumption. epigallocatechin gallate (egcg), a principal polyphenolic component of green tea, is considered key to these healthful qualities. although numerous studies have evaluated the anti-cancer effects of green tea and egcg, few have examined the safety of egcg consumption. the genotoxic potential of a concentrated egcg preparation was tested in salmonella and l5178y tk+/- mouse lymphoma cell assa ...200616364532
genetic integrity of somaclonal variants in tea (camellia sinensis (l.) o kuntze) as revealed by inter simple sequence repeats.adoption of inter simple sequence repeats (issr) technique to analyze the genetic variability of somatic embryo derived tea plants was evaluated. morphological characterisation of the field grown plants revealed no identical character aligning with the parent, upasi-10. out of 40 primers, 15 exhibited concurrent polymorphism were selected for the study. genetic variability of somaclones derived from single line cotyledonary culture ranged from 33.0 to 55.0%. a unique fragment of 1.2kb was visibl ...200616360228
tealeaves may release or absorb fluoride, depending on the fluoride content of water.as the tea plant (camellia sinensis) is known to accumulate fluoride from the soil, the tealeaves may contain high concentrations of fluoride, which is easily released during infusion. in this study, we have tested the possible effect of original fluoride concentration in the water on the fluoride release from tea. moreover, we wanted to test the possible capacity of tealeaves (commercially available tea) to absorb fluoride from high-fluoride water. in low-fluoride water, fluoride is easily rele ...200616356534
theaflavin-3, 3'-digallate induces epidermal growth factor receptor downregulation.black tea is one of the most popular beverages worldwide and especially in western nations. theaflavins, a mixture of theaflavin (tf-1), theaflavin-3-gallate (tf-2a), theaflavin-3'-gallate (tf-2b), and theaflavin-3,3'-digallate (tf-3) are the major components of black tea. among these black tea components, theaflavin is generally considered to be the more effective component for the inhibition of carcinogenesis. recently, tf-3 has been shown to have an antiproliferative effect on tumor cells, bu ...200616353237
tea consumption and ovarian cancer risk in a population-based cohort.substantial evidence from laboratory studies indicates that green and black tea preparations may protect against various cancers. few epidemiologic studies, however, have examined the relationship specifically between tea consumption and risk of ovarian cancer.201616344429
copper(ii)-selective fluorimetric bulk optode membrane based on a 1-hydroxy-9,10-anthraquinone derivative having two propenyl arms as a neutral fluorogenic ionophore.a new optical chemical sensor has been developed for the selective determination of copper(ii) ions in aqueous solutions. the reversible sensing system was prepared by incorporating 1-hydroxy-2-(prop-2'-enyl)-4-(prop-2'-enyloxy)-9,10-anthraquinone (aq) as a neutral cu2+-selective fluoroionophore in the plasticized pvc membrane with potassium tetrakis(p-chlorophenyl borate) as an anionic additive. the response of the sensor is based on the fluorescence quenching of aq by cu2+ ions. at a ph 5.5, t ...200616344241
changing diversity of hymenopteran parasitoids from organically and conventionally managed tea-ecosystem of north bengal, india.tea, camellia sinensis (l.) o. kuntze, is grown conventionally (application of chemical fertilizer and pesticides) as well as organically in darjeeling hills and adjoining plains. studies on parasitic hymenoptera gain relevance due to their effective role as biocontrol agents for maintenance of ecological balance and as biological indicators of health of tea agro-ecosystem. diversity of the hymenopterans was studied using shannon-weaver index (1963) both at morphospecies and family levels. the a ...200516334289
stereoselective oxidation at c-4 of flavans by the endophytic fungus diaporthe sp. isolated from a tea plant.the microbial transformation of five flavans (1-5) by endophytic fungi isolated from the tea plant camellia sinensis was investigated. it was found that the endophytic filamentous fungus diaporthe sp. oxidized stereoselectively at c-4 position of (+)-catechin (1) and (-)-epicatechin (2) to give the correspondent 3,4-cis-dihydroxyflavan derivatives (6, 10), respectively. (-)-epicatechin 3-o-gallate (3) and (-)-epigallocatechin 3-o-gallate (4) were also oxidized by the fungus into 3,4-dihydroxyfla ...200516327190
bioactive saponins and glycosides. xxiii. triterpene saponins with gastroprotective effect from the seeds of camellia sinensis--theasaponins e3, e4, e5, e6, and e7.the saponin fraction from the seeds of the tea plant [camellia sinensis (l.) o. kuntze (theaceae)] was found to exhibit potent protective effects on ethanol- and indomethacin-induced gastric mucosal lesions in rats. five new triterpene saponins, theasaponins e3 (1), e4 (2), e5 (3), e6 (4), and e7 (5), were isolated together with 11 known saponins from the saponin fraction. the chemical structures of 1-5 were elucidated on the basis of chemical and physicochemical evidence. among the isolated sap ...200516327189
a clinical study: melaleuca, manuka, calendula and green tea mouth rinse.a novel mouthrinse (ind 61,164) containing essential oils and extracts from four plant species (melaleuca alternifolia, leptospermum scoparium, calendula officinalis and camellia sinensis) were tested. this study aimed to evaluate the safety, palatability and preliminary efficacy of the rinse. fifteen subjects completed the phase i safety study. seventeen subjects completed the phase ii randomized placebo-controlled study. plaque was collected, gingival and plaque indices were recorded (baseline ...200516317652
effect of polymeric black tea polyphenols on benzo(a)pyrene [b(a)p]-induced cytochrome p4501a1 and 1a2 in mice.the chemopreventive activity of green tea polyphenols (gtps) is, in part, due to modulation of cytochrome p450s (cyps). to investigate the enzyme modulatory properties of major black tea polyphenols, the effect of decaffeinated black tea extract (dbte) or polymeric black tea polyphenol (pbp) mix was studied on cyp1a1 and cyp1a2 in mouse tissues. animals receiving 2.5% dbte or 1% pbp mix or drinking water (15 days) were challenged with single oral benzo(a)pyrene (b(a)p) (1 mg/mouse) treatment on ...200516316927
anticlastogenic effects of black tea polyphenols theaflavins and thearubigins in human lymphocytes in vitro.black tea accounts for nearly 80% of total world tea production. it contains dimeric flavanols and polymeric polyphenols known as theaflavins (tf) and thearubigins (tr). tr is exclusively present in black tea. on the basis of our previous potent antimutagenic and anticlastogenic effects of tf and tr in vitro in bacterial system and in vivo in mouse bone marrow cells, we have decided to extend our study in human cells in vitro. this study investigated the anticlastogenic effects of black tea poly ...200616314069
green tea, black tea and breast cancer risk: a meta-analysis of epidemiological studies.experimental studies have shown that tea and tea polyphenols have anti-carcinogenic properties against breast cancer. a number of epidemiologic studies, both case-control and cohort in design, have examined the possible association between tea intake and breast cancer development in humans. this meta-analysis included 13 papers which examined populations in eight countries and provided data on consumption of either green tea or black tea, or both in relation to breast cancer risk. summary odds r ...200616311246
copper(ii)-selective fluorimetric bulk optode membrane based on a 1-hydroxy-9,10-anthraquinone derivative having two propenyl arms as a neutral fluorogenic ionophore.a new optical chemical sensor has been developed for the selective determination of copper(ii) ions in aqueous solutions. the reversible sensing system was prepared by incorporating 1-hydrpxy-2-(prop-2'-enyl)-4-(prop-2'-enyloxy)-9,10-anthraquinone (aq) as a neutral cu2+-selective fluoroionophore in the plasticized pvc membrane with potassium tetrakis(p-chlorophenyl borate) as an anionic additive. the response of the sensor is based on the fluorescence quenching of aq by cu2+ ions. at a ph 5.5, t ...200616307904
in vitro cytotoxicity of a theaflavin mixture from black tea to malignant, immortalized, and normal cells from the human oral cavity.the growth inhibitory effects of a theaflavin mixture from black tea were more pronounced to malignant (cal27; hsc-2; hsg1) and immortalized (s-g; gt1) cells than to normal (hgf-2) cells from the human oral cavity. studies with malignant carcinoma cal27 cells and immortalized gt1 fibroblasts showed that cytotoxicity of the theaflavin mixture was enhanced as the exposure time was increased, with the tumor cal27 cells more sensitive than the gt1 cells. hydrogen peroxide (h(2)o(2)) was detected in ...200616297594
antioxidant activity of various teas against free radicals and ldl oxidation.tea is a widely consumed beverage throughout the world. we assessed the antioxidant activity of six teas, including the aqueous extracts of green tea and oolong tea (camellia sinensis), tochu (eucommia ulmoides), gymnema sylvestre, japanese mugwort (artemisia princeps), and barley (hordeum vulgare), against 1,1-diphenyl-2-picrylhydrazyl (dpph) radicals and ldl oxidation, and examined the association of ldl oxidizability with the plasma catechin levels in 10 healthy volunteers with a single dose ...200516296404
aqueous extract of black tea (camellia sinensis) prevents ethanol+cholecystokinin-induced pancreatitis in a rat model.black tea extract (bte), a phytocompound has been attributed with a plethora of health-promoting actions. we have previously demonstrated that bte inhibits chronic hepatitis in a rat model induced with high-fat and ethanol (etoh). this study reports that bte prevents altered pancreatic acinar cell functions, oxidative stress, inflammatory changes and dna damage in the etoh+cholecystokinin (cck)-induced model of pancreatitis. the etoh+cck model rats were administered with bte, and were examined t ...200616289561
in vitro and in vivo antimicrobial action of tea: the commonest beverage of asia.the methanolic extract of leaves of camellia sinensis (l) o. kuntze was screened for antimicrobial property against 111 bacteria comprising 2 genera of gram positive and 7 genera of gram negative bacteria. most of these strains were inhibited by the compound at 10-50 microg/ml level and few strains were sensitive even at lower concentrations (5 microg/ml). the bacteria could be arranged in the decreasing order of sensitivity towards the compound in the following manner: staphylococcus aureus, vi ...200516272702
more tea for septic patients?--green tea may reduce endotoxin-induced release of high mobility group box 1 and other pro-inflammatory cytokines.despite recent advances in antibiotic therapy and intensive care, sepsis remains widespread problems in critically ill patients. the high mortality of sepsis is in part mediated by bacterial endotoxin, which stimulates macrophages/monocytes to sequentially release early (e.g., tnf, il-1, and ifn-gamma) and late (e.g., hmgb1) pro-inflammatory cytokines. in light of our recent discovery of hmgb1 as a late mediator of lethal systemic inflammation, and the observation that green tea (camellia sinens ...200616266789
oral administration of a decaffeinated green tea (camellia sinensis) extract did not alter urinary 8-epi-prostaglandin f(2 alpha), a biomarker for in-vivo lipid peroxidation.oxidative stress is involved in the pathogenesis of numerous chronic human diseases. the objective of this study was to determine whether administration of a decaffeinated green tea extract providing 844 mg flavonoids daily reduced the urinary excretion of 8-epi-prostaglandin f(2 alpha) (8-epi-pgf(2 alpha)), a product of lipid peroxidation in cellular membranes and of low-density lipoprotein (ldl). nine healthy male and female subjects were studied at baseline and after 14 days of green tea supp ...200516259767
black tea may be as good as green. 200516259081
[rapd analysis on genetic diversity of the preconcentrated core germplasms of camellia sinensis in china].the study was to evaluate the genetic diversity of 69 tea cultivars of the preconcentrated core germplasms of camellia sinensis in china by the random amplified polymorphic dna (rapd). among 50 arbitrary primers, 32 primers could generate enough amplified bands for all the strains in this study. among a total of 348 bands observed, 328 (94.3%)bands were polymorphic in the 69 cultivars tested except additional 20 cultivars. genetic distances between the cultivars varied from 0.223 to 0.723. the s ...200516257906
induction of apoptosis in human leukemia cells by black tea and its polyphenol theaflavin.treatment of human leukemic cell lines hl-60 and k-562 with extracts of green and black tea and their polyphenols epigallocatechin gallate and theaflavins, respectively, showed a dose dependent inhibition of growth as a result of cytotoxicity and suppression of cell proliferation. based on the ic50 values obtained from cytotoxicity data it was clearly evident that black tea was as efficient as green tea. analysis of polyphenol contents of tea extracts revealed that not only epigallocatechin gall ...200516253767
flavor characteristics of lapsang souchong and smoked lapsang souchong, a special chinese black tea with pine smoking process.the major volatile constituents of lapsang souchong, smoked lapsang souchong, and smoked souchong, a group of special black teas in china, were analyzed with gc and gc-ms analyses. forty-nine constituents were identified. longifolene and alpha-terpineol were the most abundant compounds in the aroma. due to its special production process, the compounds longifolene, longicyclene, guaiacol, 4-methylguaiacol, 4-ethylguaiacol, etc., were identified only in this kind of black tea. the aroma constituen ...200516248572
puerins a and b, two new 8-c substituted flavan-3-ols from pu-er tea.pu-er tea is a special treated fermented tea produced from crude green tea, which is prepared from the leaves of camellia sinensis var. assamica. it is a traditional beverage having been used in china, particularly the southern areas, for a long time. chemical investigation led to the identification of two new 8-c substituted flavan-3-ols, puerins a (1) and b (2), and two known cinchonain-type phenols, epicatechin-[7,8-bc]-4alpha-(4-hydroxyphenyl)-dihydro-2(3h)-pyranone (3) and cinchonain ib (4) ...200516248561
analytical separation of tea catechins and food-related polyphenols by high-speed counter-current chromatography.high-speed counter-current chromatography (hsccc) using the type-j coil planet centrifuge was applied to compositional analysis of tea catechins and separation of other food-related polyphenols. the hsccc separation of nine different standard compounds and those from extracts of commercial tea leaves was performed with a two-phase solvent system composed of tert-butyl methyl ether-acetonitrile-0.1% aqueous trifluoroacetic acid (tfa) (2:2:3, v/v/v) by eluting the upper organic phase at a flow rat ...200616239007
camellia sinensis: historical perspectives and future prospects.as the source of brewed tea preparations, camellia sinensis has operated for centuries at the interface of agriculture, politics, and biology. the purpose of this review is to present an inter-disciplinary survey of issues surrounding cultivation, processing, and consumption of the tea plant. particular attention is given to the biologic effects of tea: first, assessing at the level of human physiology its value as a chemopreventive and chemotherapeutic agent; second, examining effects of produc ...200516236672
antifungal activity of plant extracts against arthrinium sacchari and chaetomium funicola.various plant extracts were examined for antifungal activity with the objective of improving the commercial sterility of aseptically filled tea beverage products in pet bottles. when the hot water extract and the methanol extract of 29 samples were measured for their antifungal activity against arthrinium sacchari m001 and chaetomium funicola m002 strains, five samples, acer nikoense, glycyrrhiza glabra, lagerstroemia speciosa, psidium guajava and thea sinensis, showed high activity. of these, t ...200016232887
arsenic removal from groundwater by pretreated waste tea fungal biomass.arsenic contamination in ground water poses a serious threat on human health. the tea fungus, a waste produced during black tea fermentation has been examined for its capacity to sequester the metal ions from ground water samples. autoclaved tea fungal mat and autoclaving followed by fecl3 pretreated tea fungal mat were exploited for removal of as(iii), as(v) and fe(ii) from ground water sample collected from kolkata, west bengal, india. the biosorption rate tends to increase with the increase i ...200616216732
keemun black tea extract contains potent fatty acid synthase inhibitors and reduces food intake and body weight of rats via oral administration.the inhibitory effects of a black tea extract on fatty acid synthase were measured through inhibition kinetics. the keemun black tea extract showed more potent inhibitory activity on fatty acid synthase than green tea extract. additionally, the inhibitory ability of the black tea extract depended on the extracting solvent and the conditions used. only 10-23% of the inhibitory activity from the black tea was extracted by the general method of boiling with water. the results suggested that the mai ...200516206829
dose-dependent levels of epigallocatechin-3-gallate in human colon cancer cells and mouse plasma and tissues.epigallocatechin-3-gallate (egcg; molecular formula: c22h18011)is the most abundant catechin in green tea (camellia sinensis theaceae). both egcg and green tea have been shown to have cancer-preventive activity in a number of animal models, and numerous mechanisms have been proposed based on studies with human cell lines. egcg has been shown to undergo extensive biotransformation to yield methylated and glucuronidated metabolites in mice, rats, and humans. in the present study, we determined the ...200616204466
green tea leaf extract improves lipid and glucose homeostasis in a fructose-fed insulin-resistant hamster model.the present study evaluated the effect of green tea (camellia sinensis l.) leaf extract on triglyceride and glucose homeostasis in a fructose-fed hypertriglyceridemic, insulin-resistant hamster model. there was a significant decrease in plasma triglyceride levels following supplementation of the green tea epigallocatechin gallate-enriched extract (42% at 150 mg/(kg day) to 62% at 300 mg/(kg day) for 4 weeks). compared to baseline, the fructose control group at the end of the study showed elevate ...200616202550
hepatoprotective effect of green tea (camellia sinensis) extract against tamoxifen-induced liver injury in rats.tamoxifen citrate (tam), is widely used for treatment of breast cancer. it showed a degree of hepatic carcinogenesis. the purpose of this study was to elucidate the antioxidant capacity of green tea (camellia sinensis) extract (gte) against tam-induced liver injury. a model of liver injury in female rats was done by intraperitoneal injection of tam in a dose of 45mg kg(-1) day(-1), i.p. for 7 successive days. gte in the concentration of 1.5 %, was orally administered 4 days prior and 14 days aft ...200516202236
cytotoxic and apoptogenic effect of tea (camellia sinensis var. assamica) root extract (tre) and two of its steroidal saponins ts1 and ts2 on human leukemic cell lines k562 and u937 and on cells of cml and all patients.the anticancer activity of di- and tri-terpenes and other polyphenolic compounds present in tea is already reported. we evaluated the cytotoxic and apoptogenic effect of tea root extract (tre) and two of its steroidal saponins named as ts1 and ts2, on human cell lines and on cells from leukemic patients. it was found that tre, ts1 and ts2 significantly decreased cell count and that tre caused apoptosis, as confirmed morphologically by confocal microscopy and by flow-cytometric analysis using ann ...200616197994
modulation of mpp+ uptake by tea and some of its components in caco-2 cells.the entry of most xeno/endobiotics into the organism is limited by their intestinal absorption. the interference of certain foods with the therapeutic efficacy of drugs or with chemical toxicity is becoming evident and growing attention is being given to these subjects. the aim of this work was to study the effect of green tea (gt) and black tea (bt), as well as some of their components, on the transport of organic cation molecules. for this purpose, 3h-mpp+ (radiolabeled 1-methyl-4-phenylpyridi ...200516193318
oxidative deamination of benzylamine and lysine residue in bovine serum albumin by green tea, black tea, and coffee.oxidative deamination by various polyphenolic compounds is presumed to be due to the oxidative conversion of polyphenols to the corresponding quinones through autoxidation. here we examined the oxidative deamination by the polyphenol-rich beverages green tea, black tea, and coffee at a physiological ph and temperature. green tea, black tea, and coffee extracts oxidatively deaminated benzylamine and the lysine residues of bovine serum albumin to benzaldehyde and alpha-aminoadipic delta-semialdehy ...200516190665
creaming in black tea.tea cream is the precipitate formed as tea cools. its formation has been studied by x-ray scattering, and it is shown that a higher tea concentration leads to earlier onset of creaming and larger particles and that addition of theaflavin and calcium promotes creaming. association constants between the major components of black tea have been obtained using nmr and show that calcium and glucose enhance the self-association of caffeine, polyphenols, and theaflavin but have little effect on hetero-a ...200516190662
[chemical and sensory characterization of tea (thea sinensis) consumed in chile].by means of descriptive analysis four varieties of tea (thea sinensis) were assesed: argentinean op (orange pekoe) tea (black), brazilian op tea (black), ceylan op tea (black) and darjeeling op tea (green). the appearance of dry tea leaves were qualitatively characterized comparing with dry leaves standard. the attributes: colour, form, regularity of the leaves, fibre and stem cutting were evaluated the differences obtained were related to the differences produced by the effect of the fermentati ...200516187684
floratheasaponins a-c, acylated oleanane-type triterpene oligoglycosides with anti-hyperlipidemic activities from flowers of the tea plant (camellia sinensis).the methanolic extract and its n-butanol-soluble fraction from the flowers of the tea plant (camellia sinensis) were found to suppress serum triglyceride elevation in olive oil-treated mice. from the n-butanol-soluble fraction, three new acylated oleanane-type triterpene oligoglycosides, floratheasaponins a-c (1-3), were isolated together with several flavonol glycosides and catechins. the structures of 1-3 were elucidated on the basis of chemical and physicochemical evidence as 21-o-angeloyl-22 ...200516180814
effect of herbal polyphenols on atherogenic transcriptome.the ancient indian system of medicine supports the antiatherogenic properties of some herbs. the crosstalk amongst the genes coding for ldlr, lxralpha, ppars (alpha,gamma), cd-36 and c-myc may be important in atherogenesis because these genes control lipid metabolism, cytokine production and cellular activity within the arterial wall. hence, we attempted for the first time to explore whether or not the polyphenols extracted from medicinal herbs had any effect on the transcription of these genes. ...200516180103
novel treatments for obesity and osteoporosis: targeting apoptotic pathways in adipocytes.obesity and osteoporosis have grave consequences for human health, quality of life, and even the efficiency of the labor force and economy. however, these pathologies share a common cell progenitor, revealing a surprising target for drug research and development. recent findings show that high adipocyte count in bone marrow is directly related to bone loss, as fat cells replace osteoblasts (or bone-forming cells). the objective of this review is to examine the importance of adipocyte apoptosis i ...200516178781
a novel black tea pigment and two new oxidation products of epigallocatechin-3-o-gallate.during tea fermentation, oxidation-reduction dismutation of a number of quinone metabolites of tea catechins yields numerous minor products, which make it difficult to separate and purify black tea polyphenols. in this study, epigallocatechin-3-o-gallate was enzymatically oxidized and then the unstable quinone metabolites in the oxidation mixture were hydrogenated with 2-mercaptoethanol to reduce production of inseparable minor dismutation products. as a result, three new oxidation products incl ...200516159188
inhibitory effects of epigallocatechin gallate on compound 48/80-induced mast cell activation and passive cutaneous anaphylaxis.epigallocatechin gallate (egcg) is a principle phenolic antioxidant found in a variety of plants, including green and black tea. the anti-allergic effect of egcg is unknown. the purpose of this study is to investigate the effects of egcg on compound 48/80-induced mast cell activation and passive cutaneous anaphylaxis. for this, the influences of egcg on the compound 48/80-induced cutaneous reaction were measured in vivo and the effects of egcg on the compound 48/80-induced mast cell activations ...200516155406
fulminant hepatitis during self-medication with hydroalcoholic extract of green tea.despite an ancient reputation for potential phytotherapeutic effects and innocuity, traditional herbal medicine has previously been implicated in severe adverse events. exolise is an 80% ethanolic dry extract of green tea (camellia sinensis) standardized at 25% catechins expressed as epigallocatechin gallate, containing 5-10% caffeine. it has been available in france, belgium, spain and the united kingdom since 1999, as an adjuvant therapy for use in weight loss programmes. in various studies, g ...200516148563
[acute hepatitis induced by camellia sinensis (green tea)]. 200516145729
medicinal benefits of green tea: part ii. review of anticancer properties.currently there is wide interest in the medicinal benefits of green tea (camellia sinensis). tea is one of the most widely consumed beverages in the world, and extracts of tea leaves are also sold as dietary supplements. green tea extracts contain a unique set of catechins that possess biologic activity in antioxidant, antiangiogenesis, and antiproliferative assays that are potentially relevant to the prevention and treatment of various forms of cancer. with the increasing interest in the health ...200516131288
hplc analysis of naturally occurring methylated catechins, 3' '- and 4' '-methyl-epigallocatechin gallate, in various fresh tea leaves and commercial teas and their potent inhibitory effects on inducible nitric oxide synthase in macrophages.(-)-epigallocatechin-3-gallate (egcg), a major polyphenol of green tea, undergoes substantial biotransformation to species that includes the methylated compounds. recent studies have demonstrated that the methylated egcg has many biological activities. in this study, we have investigated the composition of the three o-methylated egcg derivatives, (-)-epigallocatechin-3-o-(3-o-methyl)gallate (3' '-me-egcg), (-)-epigallocatechin-3-o-(4-o-methyl)gallate (4' '-me-egcg) and (-)-4'-methyl epigallocate ...200516131108
risk factors for kidney cancer in a japanese population: findings from the jacc study.the incidence of kidney cancer is high in western and northern europe and north america, and low in asia. although the incidence of kidney cancer in japan is lower than the rates in the other industrialized countries, there is no doubt that it is increasing.200516127235
influence of tea drinking on manganese intake, manganese status and leucocyte expression of mnsod and cytosolic aminopeptidase p.since black tea contains high levels of manganese (mn), we investigated the relationship between dietary mn intake, circulating mn levels and leucocyte expression of two mn-dependent enzymes in tea drinkers and non-tea drinkers.200616118651
protective effects of black tea extract on testosterone induced oxidative damage in prostate.since ancient times, antipyretic, anti-inflammatory, antimicrobial and antioxidative properties of tea have been recognized. black tea (camellia sinensis) contains a variety of polyphenolic ingredients including the theaflavins (tf), thearubigins (tg) and catechins. components from black tea have been accounted to play an important role in scavenging free radicals generated by mutagens and carcinogens. androgens are the key factors in either the initiation or progression of prostate cancer (pca) ...200516112415
synergistic antimicrobial activity of tea & antibiotics.tea leaves are known for its antibacterial activity against many microorganisms. in this study we attempted to describe the synergistic antimicrobial activity of tea and antibiotics against enteropathogens. antimicrobial activity of boiled water tea extract and organic solvent extract were studied against salmonella typhimurium 1402/84, s. typhi, s. typhi ty2a, shigella dysenteriae, yersinia enterocolitica c770, and escherichia coli (epec p2 1265) determining minimum inhibitory concentration (mi ...200516106094
comparative evaluation of the chemopreventive efficacy of green and black tea polyphenols in the hamster buccal pouch carcinogenesis model.to evaluate the comparative chemopreventive efficacy of green tea polyphenols (polyphenon-e) and black tea polyphenols (polyphenon-b) on 7,12-dimethylbenz[a]anthracene (dmba)-induced hamster buccal pouch (hbp) carcinogenesis.200516098960
associations between black tea and coffee consumption and risk of lung cancer among current and former smokers.although cigarette smoking is a clear risk factor for lung cancer, the other determinants of lung cancer risk among smokers are less clear. tea and coffee contain catechins and flavonoids, which have been shown to exhibit anticarcinogenic properties. conversely, caffeine may elevate cancer risk through a variety of mechanisms. the current study investigated the effects of regular consumption of black tea and coffee on lung cancer risk among 993 current and former smokers with primary incident lu ...200516090999
separation of catechin constituents from five tea cultivars using high-speed counter-current chromatography.catechins were extracted from five different tea (camellia sinensis l.) cultivars. high-speed counter-current chromatography was found to be an efficient method for the separation of seven catechins from the catechin extracts. high-performance liquid chromatography was used to assess the purity of the catechins isolated. epigallocatechin gallate (egcg), epicatechin gallate (ecg) and epigallocatechin (egc) of high purity (91-99%) were isolated in high yield after a single high-speed counter-curre ...200516078712
inhibitory effects of various beverages on ritodrine sulfation by recombinant human sulfotransferase isoforms sult1a1 and sult1a3.ritodrine is known to undergo extensive presystemic sulfation in the intestinal mucosa, and its bioavailability is as low as 30%. accordingly, inhibition of intestinal sulfation may lead to an increase in the bioavailability of ritodrine. in this study, we aimed to investigate the activities of ritodrine sulfation by sult1a1, which is expressed predominantly in the liver, and sult1a3, which is expressed predominantly in the intestine, as well as the inhibitory effects of beverages on their activ ...200516078151
xanthine oxidase activity in vitro: effects of food extracts and components.there is significant interest in the direct antioxidant activities of dietary polyphenols, due to associations between consumption of polyphenol-rich foods, such as fruits and vegetables, and decreased incidence of oxidative-stress related disease. however, indirect antioxidant action, such as the inhibition of ros-producing enzymes, may be equally relevant to health benefits through a general reduction in oxidative stress in vivo. to this end, the effects of food extracts and individual compoun ...200516076142
seasonal variations of phenolic compounds in australia-grown tea (camellia sinensis).seasonal variations of phenolic compounds in fresh tea shoots grown in australia were studied using an hplc method. three principal tea flavanols [epigallocatechin gallate (egcg), epicatechin gallate (ecg), and epigallocatechin (egc)] and four grouped phenolics [total catechins (cs), total catechin gallates (cgs), total flavanols (fla), and total polyphenols (pps)] in fresh tea shoots were analyzed and compared during the commercial harvest seasons from april 2000 to may 2001. the levels of egcg ...200516076137
supplementation of waste tea fungal biomass as a dietary ingredient for broiler chicks.the waste tea fungal biomass produced during black tea fermentation was investigated as a dietary ingredient in poultry feeds. a small portion of fungal mat was used as starter culture for the next cycle while the major portion is discarded as waste. hence a trial study was carried out to utilize the waste fungal biomass as a supplementary diet for broiler chicks. the fungal biomass contained 179.38 g of crude protein, 120 g crude fibre, 4.82 g phosphorus, 6.56 g of calcium and 8.92 mj metaboliz ...200516051080
black tea extract can modulate protein expression of h-ras, c-myc, p53, and bcl-2 genes during pulmonary hyperplasia, dysplasia, and carcinoma in situ.lung cancer has emerged as one of the leading causes of cancer death in most developed and many developing countries of the world. in the absence of effective screening and early detection methods of lung cancer and overall poor prognosis, the 5-year survival following treatment has not improved significantly over the last two decades. it is hoped that the risk of the disease can be minimized by preventive measures. one aspect of lung cancer prevention emphasizes the cessation of tobacco smoking ...200516050805
use of black tea in modulating clastogenic effects of arsenic in mice in vivo.the concentration of arsenic in drinking water has far exceeded the permissible limit of 0.001 mg/l and has reached epidemic proportions, with a maximum of 3.7 mg/l in several districts of west bengal and in the adjoining bangladesh. because inorganic arsenic is a documented human carcinogen, arsenic in drinking water may cause 200,000-270,000 deaths per year from cancer in india alone. tea has a protective effect against the clastogenicity of arsenic. we investigated whether tea extracts could ...200516050804
[tea and coffee consumption among russian population].aim of present study was the getting data on black and green tea and coffee consumption among russian population in 1995-2003. the consumption of beverages tea and coffee was estimated by 24-hour recall of dietary intake in course of russian longitudinal monitoring surveys of economics and health. about 82% of population have consumed the black tea and only 2.2%--the green tea. mean daily black and green tea intake among all population were 446 ml and 10 ml, correspondingly. the prevalence of bl ...200516044841
cosmeceuticals containing herbs: fact, fiction, and future.modern medicine is rooted in ethnobotanical traditions using indigenous flora to treat symptoms of human diseases or to improve specific aspects of the body condition. herbal medicine is now used by over half of the american population. yet the american medical community generally lacks knowledge of the function, metabolism, interaction, adverse reactions, and preparation of herbal products.200516029681
stability of black tea polyphenol, theaflavin, and identification of theanaphthoquinone as its major radical reaction product.in the current study, we have focused on isolation and detection of major radical oxidation products from theaflavin in order to better understand antioxidation mechanisms of this compound. theanaphthoquinone was identified as a major oxidation product of theaflavin from two different oxidant model systems: dpph and peroxidase/hydrogen peroxide. this result indicated that the benzotropolone moiety in theaflavin may play an important role in its antioxidant properties. the stability of theaflavin ...200516029009
addition of milk does not alter the antioxidant activity of black tea.tea is a polyphenol-rich beverage like wine and catechins are its chief polyphenols. catechins have cardio-protective effects as they can scavenge free radicals and inhibit lipid peroxidation. epidemiological studies indicate an inverse relation between tea consumption and the risk of cardiovascular and other chronic diseases. addition of milk to black tea has been reported to adversely affect its beneficial effects, but the data are not unequivocal. therefore, we assessed the effect of the addi ...200516020939
epigallocatechin gallate (egcg) (teavigo) does not impair nonhaem-iron absorption in man.a number of studies have shown that tea catechins can inhibit intestinal iron absorption, mostly iron in the nonhaem form. this randomized, double-blind, placebo-controlled, 3-periods cross-over study examined the degree of inhibition of nonhaem iron absorption by pure crystalline epigallocatechin gallate (egcg). the study was designed to show the maximum inhibitory action of egcg by selecting 30 healthy women with low iron stores. treatments were 150 mg, 300 mg egcg and placebo each for 8 conse ...200516008116
phytoestrogenic effects of black tea extract (camellia sinensis) in an oophorectomized rat (rattus norvegicus) model of osteoporosis.the adverse side effects of currently available anti-osteoporotic agents warrant the search for compounds with less toxic effects. in this study, we assessed the phytoestrogenic potentiality of whole aqueous extract of black tea (bte) in a bilaterally oophorectomized rat model (2.5%, 1 ml/100 g body weight/day for 28 days). although the supplementation was given for 28 days but, sign of revival of copulation period (estrous stage) from non-receptive diestrous stage was first noticed after 21 day ...200515996685
medicinal benefits of green tea: part i. review of noncancer health benefits.tea, in the form of green or black tea, is one of the most widely consumed beverages in the world. extracts of tea leaves also are sold as dietary supplements. however, with the increasing interest in the health properties of tea and a significant rise in scientific investigation, this review covers recent findings on the medicinal properties and noncancer health benefits of both green and black tea. in part ii, a review of anticancer properties of green tea extracts is presented. green tea cont ...200515992239
protective effect of black tea extract on the levels of lipid peroxidation and antioxidant enzymes in liver of mice with pesticide-induced liver injury.sub-acute hepatotoxicity was induced in mice by exposure to pesticides. the effect of pretreatment with aqueous black tea extract on lipid peroxidation and antioxidants in the liver was investigated. administering a combination dose of chlorpyriphos and cypermethrin (20 mg kg(-1) each) on alternate days over a 15-day period to male mice resulted in induction of sub-acute toxicity as reflected by elevated levels of liver damage marker enzymes alkaline phosphatase(alp), aspartate transaminase(ast) ...200615991261
all teas are not created equal: the chinese green tea and cardiovascular health.tea is one of the most widely consumed beverages in the world, next only to water. it can be categorized into three types, depending on the level of fermentation, i.e., green (unfermented), oolong (partially fermented) and black (fermented) tea. in general, green tea has been found to be superior to black tea in terms of antioxidant activity owing to the higher content of (-)-epigallocatechin gallate. the processes used in the manufacture of black tea are known to decrease levels of the monometr ...200615978686
about the cover illustration: thea sinensis - the common tea plant. 200515976762
molecular definition of black tea taste by means of quantitative studies, taste reconstitution, and omission experiments.recently, bioresponse-guided fractionation of black tea infusions indicated that neither the high molecular weight thearubigens nor the theaflavins, but a series of 14 flavon-3-ol glycopyranosides besides some catechins, might be important contributors to black tea taste. to further bridge the gap between pure structural chemistry and human taste perception, in the present investigation 51 putative taste compounds have been quantified in a black tea infusion, and their dose-over-threshold (dot) ...200515969522
[the green tea, a good choice for cardiovascular disease prevention?].tea (camellia sinensis) has been used for centuries as a medical drink. around two-thirds of the world's population drink tea. it is originated from southern china and entensive cultivated in asia and in central african countries. tea can be grouped into three main types, black, oolong, and green tea. green tea is not fermented and is a major beverage consumed in asian countries. green tea is produced from freshly harvest leaves of the tea plant and they contain water, proteins, carbohydrates, m ...200415969262
determination of some organochlorine compounds in herbal colouring agent henna (lawsonia inermis) and in tea (thea sinensis).henna (lawsonia inermis) has been used for centuries as a herbal hair and skin dye, but very little is known about its additives and contaminants that could adversely affect human health. an analytical method was developed to determine organochlorine compounds in henna, as they are still widely used in the areas where henna is grown. samples were sonicated with n-hexane, extracts cleansed on florisil sorbent and analysed using gas chromatography with electron capture detection. the overall recov ...200515969202
purification and partial characterization of beta-glucosidase from fresh leaves of tea plants (camellia sinensis (l.) o. kuntze).beta-glucosidases are important in the formation of floral tea aroma and the development of resistance to pathogens and herbivores in tea plants. a novel beta-glucosidase was purified 117-fold to homogeneity, with a yield of 1.26%, from tea leaves by chilled acetone and ammonium sulfate precipitation, ion exchange chromatography (cm-sephadex c-50) and fast protein liquid chromatography (fplc; superdex 75, resource s). the enzyme was a monomeric protein with specific activity of 2.57 u/mg. the mo ...200515944750
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