Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| specific detection of mycobacterium paratuberculosis dna associated with granulomatous tissue in crohn's disease. | the role of mycobacteria, specifically mycobacterium paratuberculosis, in crohn's disease has aroused considerable controversy for many years. using the ultra sensitive polymerase chain reaction some studies have reported detection of m paratuberculosis dna in as many as 65% of crohn's disease patients but also in patients without disease. other studies have been negative for both groups. we therefore designed a double blind control trial to investigate the presence of mycobacterial dna in age, ... | 1994 | 8174989 |
| herd prevalence and geographic distribution of, and risk factors for, bovine paratuberculosis in wisconsin. | a random sample of wisconsin dairy herds, stratified by herd size, were tested for paratuberculosis by use of an absorbed elisa procedure. the elisa was optimized for overall accuracy by means of receiver operating characteristic curve analysis, and had a sensitivity and specificity of 50.9 and 94.9%, respectively. herd prevalence was analyzed for correlation with responses to a management practices questionnaire completed by the herd owners. one hundred and fifty-eight herds and 4,990 cattle we ... | 1994 | 8163422 |
| [a farm with paratuberculosis]. | 1994 | 8134912 | |
| serologic reactivity against mycobacterium paratuberculosis antigens in patients with sarcoidosis. | although sarcoidosis has clinical and histopathologic similarities to some forms of tuberculosis and other mycobacterial infections, attempts to establish a mycobacterial etiology have not been successful. using cytoplasmic antigens derived from a wild strain of mycobacterium paratuberculosis in an enzyme-linked immunosorbent assay, patients with sarcoidosis were found to have immunoglobulin levels significantly higher than those found in a control population in the igg, but not in iga or igm an ... | 1993 | 8134712 |
| abolish mycobacterium paratuberculosis strain 18. | 1993 | 8123098 | |
| investigation of mycobacterium paratuberculosis in sheep by faecal culture, dna characterisation and the polymerase chain reaction. | 1993 | 8116172 | |
| detection of mycobacterium paratuberculosis by polymerase chain reaction in children with crohn's disease. | mycobacterium paratuberculosis, the causative agent of johne's disease (a chronic enteritis in ruminants), has been suspected to be involved in crohn's disease. in this study, polymerase chain reaction was used to detect the presence of is900 dna sequences specific to m. paratuberculosis genomes in biopsies and surgical resections from 53 children with various gastrointestinal diseases and disorders. is900 sequences were found in 13 of 18 samples from patients with crohn's disease (72%; p < .01 ... | 1994 | 8106782 |
| susceptibility of balb/c, c57/b6 and c57/b10 mice to infection with mycobacterium paratuberculosis. | balb/c, c57/b10 and c57/b6 mice were examined for their susceptibility to disseminated mycobacterium paratuberculosis infection after intraperitoneal inoculation with a suspension of organisms containing mineral oil. animals were examined monthly by histopathology and bacterial tissue counts of liver and spleen over a 6-month period. only balb/c mice maintained a steady infection with an average of 4.1 x 10(5) +/- 7.8 x 10(3) and 8.1 x 10(5) +/- 2.6 x 10(4) colony forming units (cfu) per gram of ... | 1993 | 8106664 |
| abdominal lymph-node granulomas and helicobacter pylori. | 1993 | 8101316 | |
| comparison of immunohistochemistry, acid-fast staining, and cultivation for detection of mycobacterium paratuberculosis in goats. | forty-seven paired specimens of ileum and mesenterial lymph nodes from goats originating from 2 herds with paratuberculosis were investigated. culture of the specimens for mycobacterium paratuberculosis was compared with ziehl-neelsen staining and with immunohistochemical tests on paraffin-embedded tissue sections, using a m. paratuberculosis immune serum and an avidin-biotin-alkaline phosphatase method. immunohistochemical techniques detected most positive samples and produced more clearly visi ... | 1994 | 8068751 |
| mycobacterium paratuberculosis infection in two llamas. | mycobacterium paratuberculosis was diagnosed in 2 llamas by bacteriologic culturing and dna probe testing of fecal and intestinal specimens. diarrhea and weight loss were observed in both animals. lesions in the gastrointestinal tracts of the 2 llamas were similar to pathologic changes reported in cattle infected with m paratuberculosis. | 1994 | 8063605 |
| histopathological and morphometrical comparison of granulomatous lesions in balb/c and c3h/hej mice inoculated with mycobacterium paratuberculosis. | susceptibility to mycobacterium paratuberculosis strain atcc 19698 infection was compared histologically and morphometrically in balb/c (bcgs) and c3h/hej (bcgr) mice. the granulomatous lesions, containing m. paratuberculosis, in the liver and spleen of balb/c mice increased progressively until 32 weeks after intraperitoneal inoculation. in contrast, both the granulomatous lesions and number of bacteria decreased drastically in c3h/hej mice beginning at 6 weeks after inoculation. there were hist ... | 1994 | 8056872 |
| [does crohn's disease an infectious origin?]. | 1993 | 8053758 | |
| distribution of anti-cd68 (ebm11) immunoreactivity in formalin-fixed, paraffin-embedded bovine tissues. | a commercially acquired anti-human macrophage antibody (anti-cd68; ebm11) was used in an immunocytochemical technique to detect macrophages in formalin-fixed, paraffin-embedded tissues from cattle, pigs, humans, rats, turkeys, dogs, and cats. in healthy cattle, the antibody labeled alveolar macrophages, pulmonary intravascular cells (presumably intravascular macrophages), and macrophage-like cells in other tissues. in bovine lungs infected with pasteurella haemolytica, ebm11 antibody labeled 95% ... | 1994 | 8053129 |
| characterization of is1110, a highly mobile genetic element from mycobacterium avium. | a highly mobile insertion sequence designated is1110 was detected in mycobacterium avium strain lr541 following an observed increase in size of the plasmid plr20. genomic libraries of m. avium strains carrying either parental plr20 or the modified plasmid (plr20') were constructed and the sequence of the relevant clones was determined to characterize the insertion sequence and the target region. is1110 is a 1457 bp element lacking terminal inverted repeats, and is related to is900 (from mycobact ... | 1994 | 8052124 |
| development of species-specific enzyme-linked immunosorbent assay for diagnosis of johne's disease in cattle. | the previously described (m. de kesel, p. gilot, m.-c. misonne, m. coene, and c. cocito, j. clin. microbiol., 31:947-954, 1993) a362 recombinant polypeptide of mycobacterium paratuberculosis was used as reagent for an enzyme-linked immunosorbent assay (elisa). this elisa, which is endowed with species specificity with respect to the other mycobacteria, was applied to the analysis of bovine paratuberculosis (johne's disease), an endemic mycobacteriosis of cattle caused by m. paratuberculosis. the ... | 1994 | 8051246 |
| the role of mycobacteria in crohn's disease. | 1994 | 8046739 | |
| experimental infection of vaccinated and non-vaccinated lambs with mycobacterium paratuberculosis. | a study of the pathogenesis of paratuberculosis and the effects of vaccination was carried out on 17 rasa lambs, allocated to four groups. vaccination seemed to accelerate the progress of the infection, and led quickly to healing. the only site of early lesions was the interfollicular areas in the peyer's patches. this would suggest an explanation both for the location of paratuberculous lesions in the ileum in clinical cases, and for the lower susceptibility to infection of adult animals, in wh ... | 1994 | 8040384 |
| comparison of the 23s ribosomal rna genes and the spacer region between the 16s and 23s rrna genes of the closely related mycobacterium avium and mycobacterium paratuberculosis and the fast-growing mycobacterium phlei. | the 23s rdna sequences of mycobacterium paratuberculosis, m. avium and m. phlei and the sequences of the spacer regions between the 16s and 23s rrna genes were determined. the overall 23s rdna sequence identity between m. paratuberculosis and m. avium was 99.7% (nine mismatches), showing the very close relatedness of these mycobacteria. evolutionary distances between the five known mycobacterial 23s rrna/rdna sequences and those of other gram-positive g + c-rich bacteria were determined. the 23s ... | 1994 | 8025676 |
| evaluation of an enzyme-linked immunosorbent assay for diagnosis of paratuberculosis in goats. | a commercial rapid-absorbed elisa developed to detect antibodies to mycobacterium paratuberculosis in bovine serum was modified for use with goat serum. diagnostic sensitivity was evaluated, using a group of 163 goats from a herd with endemic paratuberculosis. blood and fecal samples were obtained simultaneously, and prevalence of shedding of m paratuberculosis in the feces was estimated by detection of dna of the mycobacterial insertion sequence, is900, using a commercial test kit. diagnostic s ... | 1994 | 8017689 |
| purification, characterization, gene sequence, and significance of a bacterioferritin from mycobacterium leprae. | the study of tissue-derived mycobacterium leprae provides insights to the immunopathology of leprosy and helps identify broad molecular features necessary for mycobacterial parasitism. a major membrane protein (mmp-ii) of in vivo-derived m. leprae previously recognized (hunter, s.w., b. rivoire, v. mehra, b.r. bloom, and p.j. brennan. 1990. j. biol. chem. 265:14065) was purified from extracts of the organism and partial amino acid sequence obtained. this information allowed recognition, within o ... | 1994 | 8006590 |
| comparison of cellular and extracellular proteins expressed by various isolates of mycobacterium paratuberculosis and other mycobacterial species. | protein expression profiles of 10 isolates of mycobacterium paratuberculosis, m avium 18 (formerly m paratuberculosis 18), and 1 isolate each of m avium serotype 2, m avium serotype 8, and m bovis bcg were examined. protein expression profiles of m paratuberculosis and m avium were similar. however, two-dimensional gel analysis of [35s]methionine-labeled cellular proteins resolved 4 proteins, with molecular mass of 28,000, 32,000, 32,000, and 42,000 daltons, which were expressed in greater amoun ... | 1994 | 7998697 |
| [humoral response to mycobacteria in patients with crohn disease]. | the recent recovery of mycobacterium paratuberculosis from tissues of patients with crohn's disease has highlighted the possible etiologic role of this microorganism in the disease. however, the immunological evidence generated by various groups supporting this hypothesis is as yet inconclusive. a specific antibody response might be masked in these patients by the wide antigenic homologies prevailing within the genus mycobacterium. the present study was undertaken with the purpose of exploring t ... | 1994 | 7997145 |
| diagnosis of paratuberculosis in dairy cattle, using enzyme-linked immunosorbent assay for detection of antibodies against mycobacterium paratuberculosis in milk. | an elisa containing lipoarabinomannan (lam) antigen was used to detect antibodies in milk and serum for diagnosis of mycobacterium paratuberculosis infection in dairy cattle. in experiment 1, milk and serum samples were obtained from 25 cows, and subjected to lam elisa testing immediately, and after 1 year of storage at -70 c. milk samples, with and without a commonly used chemical preservative, were tested. there was no significant difference in lam elisa results between fresh and frozen sample ... | 1994 | 7978627 |
| distribution and hybridization patterns of the insertion element is900 in clinical isolates of mycobacterium paratuberculosis. | reference strains and 31 clinical isolates of m. paratuberculosis, mainly from goats, were analysed for restriction fragment length polymorphism (rflp). restriction digests of bacterial dna were hybridized with a repetitive insertion sequence, is900, to obtain banding patterns for comparison of strains. twenty-five of the 31 field-strains hybridized with is900, and five hybridization patterns were identified. it was not possible to identify specific patterns for goat strains of m. paratuberculos ... | 1994 | 7941294 |
| [occurrence, economic significance and diagnosis of paratuberculosis]. | paratuberculosis is the most serious chronic bacterial disease of ruminants. regarding the increasing incidence of the disease in the czech republic in connection with changing situation in agriculture and, among other factors, with import of animals we are presenting a summary of the latest data. we assume that high economic losses and expected participation of mycobacterium paratuberculosis in crohn's disease in men will evoke a broad interest in the disease among people from various branches ... | 1994 | 7941227 |
| effect of combination therapy in mycobacterium paratuberculosis infected rabbits. | rabbits, infected with m paratuberculosis of caprine origin, were treated with streptomycin sulphate and rifampicin in combination with levamisole hydrochloride for a period of 2 months. marked clinical response including significant rise in total serum protein, albumin and globulin were recorded in treated groups. highly significant higher leucocyte migration inhibition indicating enhanced cmi reaction, occurred in rabbits treated for 4 weeks. complete elimination of m. paratuberculosis from fa ... | 1994 | 7927524 |
| paratuberculosis. | paratuberculosis (johne's disease) is a chronic, wasting, widespread mycobacteriosis of ruminants. it involves extensive mycobacterial shedding, which accounts for the high contagiousness, and ends with a fatal enteritis. decreases in weight, milk production, and fertility produce severe economic loss. the dna of the etiological agent (mycobacterium paratuberculosis) has a base composition (66 to 67% g+c) within the range of that of mycobacteria (62 to 70% g+c), a size (4.4 x 10(6) to 4.7 x 10(6 ... | 1994 | 7923053 |
| identification and characterization of a putative serine protease expressed in vivo by mycobacterium avium subsp. paratuberculosis. | a putative serine protease expressed in vivo by mycobacterium avium subsp. paratuberculosis was isolated from a lambda gt11 genomic expression library by screening with serum from a naturally infected sheep. the gene was contained in two overlapping clones, which were shown by antibody elution to encode a protein of 34 kda in m. a. paratuberculosis. the clones were sequenced and database searches detected a motif identical to the active serine site in trypsin, and 30% homology to the putative se ... | 1994 | 7921248 |
| use of indicators for monitoring the reduction of pathogens in animal waste treated in biogas plants. | the possibility of including bacterial indicators in programmes for monitoring the disinfection of animal waste treated in biogas plants was examined. five full-scale plants receiving waste from several farms were monitored. of these, two used thermophilic and one mesophilic digestion exclusively whereas two employed a two stage process involving thermophilic pretreatment followed by mesophilic digestion. for each plant the reducing effect on bacterial pathogens present in the incoming waste and ... | 1994 | 7916882 |
| cloning and expression in escherichia coli of dna encoding a 60 kda stress protein of mycobacterium paratuberculosis, the causative agent of johne's disease. | polymerase chain reaction (pcr) was used to generate dna encoding a 60 kda stress protein of mycobacterium paratuberculosis using primers complementary to sequences at the 5' and 3' ends of 60 kda stress protein genes (encoding the '65 kda antigens') of m. leprae and m. tuberculosis. the predicted pcr product of 1.8 kb contained the entire coding sequence of an m. paratuberculosis 60 kda stress protein, with non-coding regions of 124 bp and 1 bp at the 5' and 3' ends, respectively. dna encoding ... | 1994 | 7881551 |
| mycobacterium paratuberculosis in intestinal tissue from patients with crohn's disease demonstrated by a nested primer polymerase chain reaction. | the etiology of crohn's disease remains unknown, but current research has concentrated on autoimmunity and/or mycobacterial infection. the polymerase chain reaction (pcr) enables the detection of genetic material even when very few microorganisms are present. | 1994 | 7839099 |
| putative functional domain within orf2 on the mycobacterium insertion sequences is900 and is902. | repeated dna sequences have been identified in a range of mycobacterial species and have been implicated in the increased virulence of some of these species, namely, mycobacterium paratuberculosis and m. avium subsp. silvaticum. here we present a case to suggest that the insertion sequences is900 and is902 encode a protein from a putative gene positioned on the complementary strand to their transposase genes. based on amino acid homology analyses, this open reading frame (orf2) could encode a tr ... | 1994 | 7835987 |
| mycobacterial cell wall components induce the production of tnf-alpha, il-1, and il-6 by bovine monocytes and the murine macrophage cell line raw 264.7. | johne's disease is characterized by a chronic enteritis that results in granulomatous inflammation, cachexia, and eventual death of cattle infected with mycobacterium paratuberculosis. the cytokines tumor necrosis factor-alpha (tnf-alpha), interleukin-1 (il-1), and interleukin-6 (il-6) have been associated with granuloma formation and wasting in other disease syndromes. the potential role of these cytokines in the development and progression of johne's disease has not been investigated. freshly ... | 1994 | 7830527 |
| mycobacteria in the intestine of japanese patients with inflammatory bowel disease. | it is still controversial whether or not a mycobacterial infection may be a cause of crohn's disease. mycobacterium paratuberculosis may be very difficult to detect using routine culture techniques. to clarify this, we detected mycobacterial dna in patients with inflammatory bowel disease. | 1995 | 7801954 |
| the effect of vaccination on the prevalence of paratuberculosis in large dairy herds. | eight hundred and sixty-six one-month-old female calves were vaccinated using heat-killed mycobacterium paratuberculosis in oil during a five-year period. seven hundred and twenty-one bull calves and 379 female calves served as nonvaccinated controls. two large herds were used in the study. the calves were tested by faecal culture and by serologic tests on the day of vaccination, at 3, 6, 9 and 12 months of age, at breeding and on the day of parturition. the results were evaluated by trend analy ... | 1994 | 7801515 |
| comparison of a complement fixation test, a gel diffusion test and two absorbed and unabsorbed elisas for the diagnosis of paratuberculosis in sheep. | a complement fixation test for paratuberculosis, a gel diffusion test and two enzyme-linked immunosorbent assays (elisa) were evaluated using sera from mycobacterium paratuberculosis infected and non-infected sheep. gross pathology and histopathology were used as parameters of infection. the two elisas, one of which is commercially available for testing cattle, were used before and after sera had been absorbed with a soluble sonicate of mycobacterium phlei. differences between the various tests ... | 1994 | 7801513 |
| [value of serological determinations in the differential diagnosis of ulcerative colitis and crohn's disease, and immunogenetic aspects]. | 1994 | 7800073 | |
| comparison of polymerase chain reaction tests and faecal culture for detecting mycobacterium paratuberculosis in bovine faeces. | a polymerase chain reaction (pcr) test for m. paratuberculosis was developed based on a 218 bp segment of a dna insertion sequence, is900, that is specific for this organism. the method involved two consecutive amplification reactions, with the second set of primers being nested inside the first set. the method reliably detected 50 organisms/g faeces. this pcr test was applied to 32 bovine faecal specimens containing high, moderate or low numbers of m. paratuberculosis organisms as determined by ... | 1993 | 7794290 |
| solid-phase hybridization capture of low-abundance target dna sequences: application to the polymerase chain reaction detection of mycobacterium paratuberculosis and mycobacterium avium subsp. silvaticum. | polymerase chain reaction (pcr) has been widely applied to the detection of microorganisms. overall sensitivity of pcr tests may be substantially reduced due to a large excess of nontarget dna and inhibitory substances in the sample. we used a 5'-biotinylated 513-bp probe from the 3' region of the is 900 element specific for mycobacterium paratuberculosis (mptb) to capture target mptb dna from crude sample dna extracts. captured target dna was separated using streptavidin-coated magnetic particl ... | 1995 | 7793635 |
| phage infection, transfection and transformation of mycobacterium avium complex and mycobacterium paratuberculosis. | mycobacterium avium complex strains and mycobacterium paratuberculosis are closely related intracellular pathogens affecting humans and animals. m. avium complex infections are a leading cause of morbidity and mortality in aids patients, and m. paratuberculosis is the agent of johne's disease in ruminants. genetic manipulation of these micro-organisms would facilitate the understanding of their pathogenesis, the construction of attenuated vaccine strains and the development of new drugs and trea ... | 1995 | 7773411 |
| identification and characterization of mycobacterium paratuberculosis recombinant proteins expressed in e. coli. | mycobacterium paratuberculosis is the causative agent of johne's disease, a chronic enteritis in ruminants, and it has also been isolated and identified from patients with crohn's disease, an inflammatory bowel disease. the control of johne's disease has been hampered by the lack of a reliable diagnostic test because of the large degree of antigenic cross-reactivity between mycobacterial and non-mycobacterial species. to help identify specific antigen(s) or epitope(s), an m. paratuberculosis exp ... | 1994 | 7765093 |
| [the prevalence of paratuberculosis in the plateau de diesse region]. | we analysed 595 cows in 20 herds for serological evidence of paratuberculosis with an elisa procedure for which we had recently determined a sensitivity of 50% and a specificity of 98%, and we obtained an overall calculated real prevalence of 5.99%. in two herds with clinical cases of paratuberculosis, the calculated true prevalence of seroreactors was 21.47%. in 9 herds with clinical suspicion of johne's disease, the rate was 4.23%, 9 herds without any clinical suspicion showed a mean prevalenc ... | 1995 | 7716481 |
| the role of restricted food intake in the pathogenesis of cachexia in severe combined immunodeficient beige mice infected with mycobacterium paratuberculosis. | a paired feeding experiment was conducted to investigate if reduced food intake is a reason for the body weight loss previously observed in severe combined immunodeficient beige (scid bg) mice infected with mycobacterium paratuberculosis. mice were paired on the basis of age, litter and sex. one of each pair was injected intraperitoneally with 10(5) viable m. paratuberculosis organisms. the remainder served as uninfected pairfed mates. each uninfected mouse was restricted to the amount of food ( ... | 1995 | 7704841 |
| specificity of the immunohistochemical demonstration of mycobacterial antigens. | the specificity of immunohistological reactions of commercial polyclonal antibodies to antigens of various mycobacteria (mycobacterium bovis, mycobacterium duvalii and mycobacterium paratuberculosis) was tested in connection with a number of pathogens and with the help of the indirect immunoperoxidase technique. positive reactions occurred with various mycobacterial antigens and some gram-positive bacteria and fungi. these investigations indicate that when the above antibodies are used in histop ... | 1994 | 7701868 |
| incidence of paratuberculosis after vaccination against m. paratuberculosis in two infected dairy herds. | vaccination against paratuberculosis of all newborn animals has been performed since april 1984 in two dairy herds with a high incidence of clinical cases of paratuberculosis, using a vaccine containing heat-inactivated m. paratuberculosis in a water/mineral oil emulsion. animals slaughtered between april 1984 and january 1991 were included in the study. histology, bacterioscopy and culture on smith and modified löwenstein-jensen media were performed using jejunum, ileum and draining lymph nodes ... | 1994 | 7701865 |
| rapid detection and identification of mycobacterium avium by amplification of 16s rrna sequences. | an assay that is based on the amplification of 16s rrna sequences and that was initially developed to detect mycobacterium paratuberculosis in cattle was used to test 20 serotypes of the mycobacterium avium complex (mac) and atypical mycobacterial species not belonging to mac. only serotypes 1 to 6 and 8 to 11, designated m. avium, were detected by the assay, indicating that it can be used for the rapid detection and identification of m. avium. the results of the assay for clinical samples from ... | 1993 | 7691877 |
| cloning and expression of portions of the 34-kilodalton-protein gene of mycobacterium paratuberculosis: its application to serological analysis of johne's disease. | paratuberculosis (johne's disease), an endemic mycobacteriosis of cattle that is caused by mycobacterium paratuberculosis, is characterized by incoercible diarrhea and fecal shedding of bacteria. the present work aimed at developing a specific serological test for this disease. we have recently shown that a 34-kda protein belonging to the major antigen complex a36 of m. paratuberculosis is immunodominant and contains epitopes specific with respect to all mycobacteria tested, including mycobacter ... | 1993 | 7681851 |
| temporal effects of tumor necrosis factor-alpha on intracellular survival of mycobacterium paratuberculosis. | the causative agent in johne's disease is mycobacterium paratuberculosis, an intracellular pathogen which causes enteritis in ruminants. little is known about interactions between the host cell (macrophage) and m. paratuberculosis; however, this bacterium is able to evade normal host immune defenses and cause a chronic infective state. in the present study, we evaluated whether activation of a murine macrophage cell line (j774.16) by pretreatment with recombinant murine tumor necrosis factor alp ... | 1995 | 7676613 |
| immortalization and characterization of bovine peritoneal macrophages transfected with sv40 plasmid dna. | a transformed bovine peritoneal macrophage cell line was developed and characterized. primary peritoneal macrophages were transformed by calcium-phosphate transfection with sv40 plasmid dna. the transformed cell line retained the morphology of resident peritoneal macrophages as determined by light microscopy and histochemical analysis showed non-specific esterase activity. in addition, immunohistochemical staining of transformed peritoneal macrophages for lysozyme activity was positive. transfor ... | 1995 | 7676607 |
| johne's disease in a herd of farmed red deer. | an outbreak of johne's disease in a herd of farmed red deer was studied for four years. serological, histopathological and cultural techniques were used to monitor the progress of the disease, and delayed type hypersensitivity skin tests were also applied. the results of the serological tests showed that they were poor predictors of future clinical cases and did not consistently identify animals harbouring mycobacteria. the histopathological methods provided a sensitive and specific means of con ... | 1995 | 7661952 |
| pathogenesis of crohn's disease. | 1995 | 7648238 | |
| johne's disease in alpacas (lama pacos) in australia. | johne's disease was diagnosed in 10 alpacas (lama pacos) in australia between february 1993 and may 1994. eight of the animals were between 12 and 24 months of age, one was a 6-year-old female, and one was a 4-year-old male. five, including the 6-year-old and the 4-year-old alpacas, showed weight loss and diarrhoea before death or slaughter. the other cases showed no clinical signs of johne's disease but 4 gave a positive result on faecal culture and one gave a positive result on testing with th ... | 1995 | 7646382 |
| mycobacterium paratuberculosis and crohn's disease. | 1995 | 7615290 | |
| pathogenesis of crohn's disease: the environment revisited. | 1995 | 7614097 | |
| johne's disease in red deer. | 1995 | 7604515 | |
| diagnosis of infection with mycobacterium paratuberculosis. | 1995 | 7600511 | |
| glycosylation of igg during potentially arthritogenic lentiviral infections. | agalactosyl igg [gal(0)] was first discovered in patients with rheumatoid arthritis (ra). however, the proportion of this glycoform is also raised in tuberculosis and leprosy. this has helped reinforce the suggestion that ra may be triggered by a mycobacterium-like slow bacterial infection. on the other hand, arthritis can occur in mycobacterial diseases, so raised gal(0) could be associated with a tendency to arthritis, rather than with a particular type of infection. therefore, we wished to fi ... | 1995 | 7597380 |
| effect of intestinal resection on serum antibodies to the mycobacterial 45/48 kilodalton doublet antigen in crohn's disease. | interest in the role of mycobacterial infection in crohn's disease has been revived by the cultural detection of mycobacterium paratuberculosis in patients with crohn's disease. this hypothesis was examined serologically using assays with high specificity for crohn's disease. the effect of intestinal resection on serum antibodies specific for crohn's disease was investigated with an immunoblot assay and an enzyme linked immunosorbent assay using the 45/48 kilodalton doublet antigen of mycobacter ... | 1995 | 7590431 |
| immunoaffinity chromatographic isolation of a high molecular weight seroreactive protein from mycobacterium leprae cell sonicate. | the purpose of this study was to isolate mycobacterium leprae antigen(s) by immunoaffinity chromatography using immunoglobulins from leprosy patients and from rabbit anti-m. leprae hyperimmune serum coupled to cnbr-sepharose 4b. a high molecular weight (m(r)) m. leprae protein (mlp) with a subunit m(r) of 22,000 was isolated. mlp was recognized by monoclonal antibody mmpii1g4 which is known to react with mmpii, a 22 kda protein of m. leprae. the n-terminal sequence of the 22 kda subunit (met-gln ... | 1995 | 7581267 |
| evaluation of an enzyme-linked immunosorbent assay licensed by the usda for use in cattle for diagnosis of ovine paratuberculosis. | a commercially available mycobacterium phlei-absorbed enzyme-linked immunosorbent assay (elisa) approved to detect antibodies to mycobacterium paratuberculosis in cattle was evaluated for its applicability in sheep. the potential for interference with elisa results from cross-reacting antibodies to corynebacterium pseudotuberculosis was also investigated. serum samples were randomly selected from a collection of samples obtained in 1986-1991 from 6 infected and 5 noninfected sheep flocks varying ... | 1995 | 7578450 |
| lack of mycobactin dependence of mycobacteria isolated on middlebrook 7h11 from clinical cases of ovine paratuberculosis. | mycobacterium avium subsp. paratuberculosis is distinguished from other mycobacterial species by its dependence on the iron-chelator factor mycobactin and its prolonged incubation period when grown in vitro. traditionally, a very low rate of isolation has been considered characteristic of sheep strains of this mycobacterium species. in the present study, a comparison was made of the performance of two media, löwenstein-jensen and middlebrook 7h11/oadc both with and without mycobactin, on the iso ... | 1995 | 7571372 |
| novel glycolipids of mycobacterium avium and related m. paratuberculosis strains of relevance to aids and crohn's disease. | the polar glycolipid fractions of several mycobacterial strains of the closely related species m. avium and m. paratuberculosis have been analysed by thin layer chromatography (tlc), high ph anion exchange chromatography (hpaec), gas-liquid chromatography (gc) and nuclear magnetic resonance (nmr) spectroscopy. the upper phase of a folch partitioning (rather than the lower phase analysed by others) was subjected to tlc in solvent system chloroform-methanol-water 50:40:10 v/v/v. a major band was p ... | 1995 | 7554817 |
| recombinant bcg expressing the leishmania surface antigen gp63 induces protective immunity against leishmania major infection in balb/c mice. | we have cloned and expressed the gp63 gene of leishmania major in bcg to develop a recombinant vaccine against zoonotic cutaneous leishmaniasis. two different expression systems were investigated. the first system consists of pan, a mycobacterium paratuberculosis promoter, which drives expression of orf2, an open reading frame in is900. this system allows the production of heterologous polypeptides as hybrids with the orf2 gene product. the second expression system relies on the production of an ... | 1995 | 7551026 |
| detection of specific t cell reactivity in sheep infected with mycobacterium avium subspecies silvaticum and paratuberculosis using two defined mycobacterial antigens. | a 30 kda antigen (p30) from mycobacterium avium ssp. paratuberculosis (m. a. paratuberculosis) and a 40 kda (p40) antigen from mycobacterium avium ssp. silvaticum (m. a. silvaticum) were employed in two different assays to measure the cell-mediated immune reactivity of ovine peripheral blood lymphocytes. in lymphocyte stimulation assays, proliferative responses to the p30 were observed only with lymphocytes from sheep inoculated with live m. a. paratuberculosis or m. a. silvaticum. although this ... | 1995 | 7545852 |
| recombinant bcg strains expressing the sivmac251nef gene induce proliferative and ctl responses against nef synthetic peptides in mice. | ctl responses are known to be important for the control of hiv and siv infections. such responses are targeted against various components of these viruses including regulatory proteins like nef. the sivmac251nef gene was cloned in mycobacterium bovis bcg under the control of p(an), a promoter from mycobacterium paratuberculosis. nef was expressed as a fused polypeptide with orf2, an open reading frame adjacent to p(an). mice inoculated with rbcg(sivmac251nef) exhibited proliferative and cd8+ cyt ... | 1995 | 7543715 |
| [antigen-binding lymphocytes to the mycobacterium paratuberculosis antigen in crohn's disease and nonspecific ulcerative colitis]. | 1993 | 7521551 | |
| mycobacterium paratuberculosis and escherichia coli share common antigenic determinants. | paratuberculosis (johne's disease) is a chronic enteritis of ruminants, which is due to infection by mycobacterium paratuberculosis. by comparative analysis of western blots of m. paratuberculosis components incubated with sera from paratuberculous cows (either pre-absorbed or not on an escherichia coli sonicate), we have shown the presence of cross-reactive antigens between m. paratuberculosis and e. coli. components in the range of 22 to 75 kda are recognized by sera from paratuberculous cows, ... | 1994 | 7518988 |
| nucleotide sequence and secondary structures of precursor 16s rrna of slow-growing mycobacteria. | slow-growing mycobacteria have a single ribosomal rna (rrn) operon, with the genes for 16s, 23s and 5s rrna being present in that order. the transcription start site of the rrn operon of mycobacterium tuberculosis was identified in escherichia coli. pcr methodology was used to amplify parts of the rrn operon, namely the leader region and the spacer-1 region separating the 16s rrna and 23s rrna genes of mycobacterium avium, mycobacterium paratuberculosis, mycobacterium intracellulare, 'mycobacter ... | 1994 | 7512868 |
| identification of mycobacteria from animals by restriction enzyme analysis and direct dna cycle sequencing of polymerase chain reaction-amplified 16s rrna gene sequences. | two methods, based on analysis of the polymerase chain reaction-amplified 16s rrna gene by restriction enzyme analysis (rea) or direct cycle sequencing, were developed for rapid identification of mycobacteria isolated from animals and were compared to traditional phenotypic typing. bactec 7h12 cultures of the specimens were examined for "cording," and specific polymerase chain reaction amplification was performed to identify the presence of tubercle complex mycobacteria. combined results of sepa ... | 1993 | 7508456 |
| occurrence, in crohn's disease, of antibodies directed against a species-specific recombinant polypeptide of mycobacterium paratuberculosis. | sera from patients with crohn's disease and control were analyzed by an enzyme-linked immunosorbent assay based on the mycobacterium paratuberculosis-specific recombinant polypeptide a362. anti-a362 immunoglobulin g (igg) (p < 0.05) and iga (p < 0.001) titers were higher in patients with crohn's disease than in controls. a monomodal gaussian distribution of anti-a362 iga levels were found for controls, and a bimodal distribution was found for patients with crohn's disease. an m. paratuberculosis ... | 1994 | 7496954 |
| histopathology of c57bl/6 mice inoculated orally with mycobacterium paratuberculosis. | the susceptibility of c57bl/6 mice to oral inoculation with myobacterium paratuberculosis was evaluated histopathologically. granulomatous lesions containing acid-fast bacteria developed in the mesenteric lymph nodes in over 50% of the mice by 11 months after inoculation. the results suggest that c57bl/6 mice may be useful for studying infection, pathogenesis, and other aspects of paratuberculosis. | 1995 | 7490340 |
| characterization by restriction endonuclease analysis and dna hybridization using is900 of bovine, ovine, caprine and human dependent strains of mycobacterium paratuberculosis isolated in various localities. | dna of 90 mycobactin-dependent strains of mycobacterium paratuberculosis, isolated in 9 countries, was digested with restriction endonuclease psti and hybridized with a dna fragment containing insertion sequence is900. bovine strains (n = 73) were isolated from 61 animals in 17 herds, ovine strains (n = 15) from 13 animals in 3 herds and the set was completed by 1 caprine and 1 human (linda) strain. three types, tentatively designated a (n = 37), b (n = 51) and c (n = 2) were differentiated by r ... | 1995 | 7483244 |
| antibodies to mycobacterium paratuberculosis in intestinal fluids of sheep with johne's disease (paratuberculosis). | 1980 | 7445831 | |
| agglutinins to bacteria in crohn's disease. | sera from patients with crohn's disease were tested for antibodies against organisms which are thought to cause inflammatory bowel disease in animals, or have been implicated in human crohn's disease. control sera were collected from healthy individuals and patients with ulcerative colitis. sera from crohn's disease and controls failed to agglutinate clostridium colinum or campylobacter sputorum subsp. mucosalis and two strains of mycobacterium paratuberculosis (m26 and m27). most of the sera ag ... | 1980 | 7429299 |
| bovine paratuberculosis ii. a comparison of fecal culture and the antibody response. | fecal culture for mycobacterium paratuberculosis and a complement fixing serological test using a carbohydrate antigen were compared for diagnostic efficiency in cattle naturally infected with m. paratuberculosis. serological reactivity was associated with the persistent fecal shedding of large numbers of bacteria and in the absence of false positives in the population studied, was considered an efficient method for the identification of this segment of infected cattle. minimally infected cattle ... | 1980 | 7407691 |
| bovine paratuberculosis i. a herd study using complement fixation and intradermal tests. | a dairy herd (102 cattle) which had been enrolled under a paratuberculosis control program for two years utilizing a complement fixation test (carbohydrate antigen) and intradermal skin test (johnin ppd) was subjected to two further herd tests and followed to slaughter to determine infection status by culture and histology. mycobacterium paratuberculosis infection was demonstrated in 37 of the animals of which only five were considered reactors on the basis of the last two herd tests applied. cu ... | 1980 | 7407690 |
| bovine paratuberculosis iii. an evaluation of a whole blood lymphocyte transformation test. | a whole blood lymphocyte transformation test was used to examine cattle with varying degrees of infection with mycobacterium paratuberculosis. minimally infected animals characteristically responded to johnin purified protein derivative in the lymphocyte transformation test but did not routinely react on serological and/or skin testing. heavily infected animals showed considerable variation in their lymphocyte transformation responses to antigen and some of them were consistently unresponsive. a ... | 1981 | 7340913 |
| isolation of mycobacterium paratuberculosis from the milk of a cow with johne's disease. | 1981 | 7340090 | |
| in vitro responses of lymphocytes from cattle with advanced mycobacterium paratuberculosis infection to homologous and heterologous antigens. | the accuracy with which the purified lymphocyte and whole blood modifications of the lymphocyte stimulation test were able to detect animals with clinical johne's disease was compared with that of the complement fixation test and microscopic faecal examination. confirmatory diagnosis of johne's disease was based upon histopathological examination of intestinal tissue. false positive results were obtained only with the complement fixation test. the only test not giving rise to false negative resu ... | 1981 | 7313327 |
| effects of physiol and chemical factors on the viability of mycobacterium paratuberculosis. | alternative methods of storing bovine fecal specimens for delayed culture of mycobacterium paratuberculosis was studied. significant (p less than 0.05) losses of viability were sustained by storage at 23 degrees c for 10 days and by treatment with 0.03 zephiran for 4 and 10 days. losses were moderate after storage at -70 degrees c for 15 weeks but significant (p greater than 0.05) after storage at 23 degrees c for 4 days. the alternative methods were compared with the standard method of processi ... | 1981 | 7309854 |
| comparative lymphocyte stimulation studies on whole blood from vaccinated and nonvaccinated cattle with paratuberculosis. | the lymphocyte stimulation test was evaluated in the diagnosis of bovine paratuberculosis, using whole blood samples from cattle. these samples were incubated with a purified protein derivative of mycobacterium paratuberculosis. samples from vaccinated and nonvaccinated cattle from paratuberculosis-free herds and from paratuberculous herds were used. vaccinated and nonvaccinated groups from the paratuberculous herds each included fecal culture-positive cattle, fecal culture-negative cattle, and ... | 1981 | 7271016 |
| mycobacterium paratuberculosis in the semen and genital organs of a semen-donor bull. | mycobacterium paratuberculosis, the cause of johne's disease, was isolated from the feces of a donor bull in an artificial insemination stud. during isolation and observation for 21 months, the organism was recovered from all of 26 fecal samples and from 8 of 31 semen samples. at necropsy, it was isolated from the intestine and adjacent lymph nodes, lung, spleen, seminal vesicles, and prostate gland but not from the testicles. we concluded that routine fecal cultures at bull studs will reveal in ... | 1981 | 7263470 |
| isolation of mycobacteria inducing cross-reactions in the complement fixation test for johne's disease. | eight bulls which gave positive reactions in the complement fixation test for johne's disease were identified in a group of 29 bulls which had previously given only negative reactions at repeated annual tests. one reacting bull was confirmed to be infected with mycobacterium paratuberculosis at autopsy, but another reactor which was slaughtered had no visible lesions and mycobacteria were not recovered in culture. faecal samples from seven further bulls (six reactors and one non-reactor) yielded ... | 1981 | 7255925 |
| lack of intracellular degradation of mycobacterium paratuberculosis by bovine macrophages infected in vitro and in vivo: light microscopic and electron microscopic observations. | blood-derived macrophages from clinically healthy cows and cows infected with mycobacterium paratuberculosis were cultured in vitro and inoculated with heat-killed m paratuberculosis. five weeks after infection, acid-fast bacteria continued to be demonstrated in the macrophages. macrophage cultures also were infected with living m paratuberculosis and were examined by electron microscopy. four weeks after infection, intact bacteria were found in all cultures. electron microscopic examinations of ... | 1981 | 7224304 |
| an improved medium for culture of mycobacterium paratuberculosis from bovine faeces. | 1982 | 7180779 | |
| intrauterine inoculation of mycobacterium paratuberculosis into guinea pigs and cattle. | mycobacterium paratuberculosis was inoculated intrauterinely into guinea pigs and cattle near the time of insemination to assess the effect and subsequent distribution of the organisms. guinea pigs inoculated intrauterinely with 1 of 3 concentrations of m paratuberculosis or not inoculated were caged with male guinea pigs for 10 days. guinea pigs given the largest dose had the lowest rate of pregnancy when examined. at necropsy, tissues were cultured to determine extent of infection. abortion fo ... | 1982 | 7073089 |
| pathology of spontaneous and experimental infection of north american wild ruminants with mycobacterium paratuberculosis. | spontaneous paratuberculosis was studied in free-ranging and captive bighorn sheep (ovis canadensis), and rocky mountain goats (oreamnos americanus). lesions of paratuberculosis in these species resembled the disease in domestic sheep and goats. mycobacterium paratuberculosis cultured from bighorn sheep was used to orally infect bighorn x mouflon (ovis musimon) hybrid sheep, elk (cervus elaphus nelsoni), mule deer (odocoileus hemionus), and white-tailed deer (odocoileus virginianus). clinical pa ... | 1983 | 6879954 |
| diagnosis of johne's disease in sheep by counter-immunoelectrophoresis. | the use of counter-immunoelectrophoresis (ciep) in the diagnosis of johne's disease was investigated in flocks of sheep in iran and libya. in 50 sheep with no history of paratuberculosis none had antibodies to mycobacterium paratuberculosis. animals vaccinated as neonates were either negative or had reciprocal titres (rt) of less than 4 at 18 months of age unless exposed to infection. among 105 non-vaccinated adults in an infected flock 91% had antibodies. twenty-one animals that either died or ... | 1983 | 6845428 |
| bacteria in enteric lesions of cattle. | thirty-nine species of bacteria were isolated from or demonstrated in the abomasal and small and large intestinal mucosa of 23 adult cattle and 41 calves and identified. the bacteria isolated were related to the gross and microscopical lesions. campylobacters, clostridium perfringens type a, c sordellii, actinobacillus lignieresii, fusobacterium necrophorum, mycobacterium paratuberculosis, aeromonas hydrophila and escherichia coli were all associated with specific lesions. the relationship of ot ... | 1983 | 6829137 |
| eastern white-tailed deer as a reservoir of ruminant paratuberculosis. | ten white-tailed deer were shot and killed at a connecticut farm that had a 6-year history of bovine paratuberculosis, and organs from these animals were examined for evidence of paratuberculosis. mycobacterium paratuberculosis was isolated from the cecal lymph node, terminal ileum, and ileocecal valve of 1 deer and from the cecal lymph node of another. characteristic lesions and acid-fast bacilli were not observed. it was concluded that infected deer could serve as sources of infection for dome ... | 1983 | 6826436 |
| comparison of agar gel immunodiffusion and fecal culture for identification of goats with paratuberculosis. | a large goat herd naturally infected with mycobacterium paratuberculosis has been monitored for new infections for approximately 21/2 years, using both agar gel immunodiffusion and bacterial culture of feces. on the basis of postmortem confirmation of infection status, both methods had equal diagnostic sensitivity and specificity. the agar gel immunodiffusion test offered distinct advantages in ease of performance and rapidity of test results. it was concluded that serious consideration should b ... | 1980 | 6777351 |
| diagnosis of paratuberculosis in sheep. | paratuberculosis in sheep usually is manifested as emaciation and decreased wool production. diarrhea occurred in only 18% of affected animals. significant hematologic changes included decreased rbc count, hemoglobin level and hematocrit. necropsy revealed pallor, cachexia and serous fluid in body cavities. staining of intestinal mucosal scrapings and mesenteric lymph node impression smears for acid-fast organisms revealed bright-red clumps of mycobacterium paratuberculosis bacilli. fecal examin ... | 1984 | 6727847 |
| isolation of specific peptides from mycobacterium paratuberculosis protoplasm and their use in an enzyme-linked immunosorbent assay for the detection of paratuberculosis (johne's disease) in cattle. | an antigen was isolated from the protoplasm of mycobacterium paratuberculosis by a combination of gel filtration, ion exchange, and affinity chromatography. the purified antigen constituted 7.8% of the total protein in the protoplasm. the specificity and sensitivity of the enzyme-linked immunosorbent assay (elisa) for paratuberculosis, using the purified antigen, were evaluated with sera from 104 cattle which were examined (surveyed) for m paratuberculosis infection by fecal cultural technique. ... | 1983 | 6660610 |
| experimental infection of some north american wild ruminants and domestic sheep with mycobacterium paratuberculosis: clinical and bacteriological findings. | mycobacterium paratuberculosis originally isolated from bighorn sheep (ovis canadensis) with spontaneous paratuberculosis was used to orally inoculate rocky mountain elk (cervus elaphus nelsoni) calves, mule deer (odocoileus hemionus) fawns, white-tailed deer (odocoileus virginianus) fawns, bighorn x mouflon (ovis musimon) hybrid lambs, and domestic lambs. all experimentally exposed animals became infected. during the first year of infection, hybrid and domestic sheep were able to control the in ... | 1983 | 6644916 |
| mycolic acid patterns of some species of mycobacterium. | representative strains of some species of mycobacterium were degraded by both acid and alkaline methanolysis. two-dimensional thin-layer chromatography was used to determine the patterns of mycolic acids and other long-chain components in these methanolysates. patterns composed of alpha-, methoxy- and ketomycolates were found in mycobacterium asiaticum, mycobacterium bovis, mycobacterium gastri, mycobacterium gordonae, mycobacterium kansasii, mycobacterium marinum and mycobacterium tuberculosis; ... | 1984 | 6517656 |
| characteristics of an unclassified mycobacterium species isolated from patients with crohn's disease. | the characteristics of an unclassified mycobacterium sp. isolated from three patients with crohn's disease are presented. the organism is extremely fastidious and mycobactin dependent and may require up to 18 months of incubation for primary isolation. colony morphology is rough. characteristics are unlike those of any presently defined species. the isolates produced postive niacin, catalase, and 2-week arylsulfatase reactions and were susceptible to neotetrazolium chloride (1:40,000), streptomy ... | 1984 | 6511878 |
| possible role of mycobacteria in inflammatory bowel disease. i. an unclassified mycobacterium species isolated from patients with crohn's disease. | a previously unrecognized mycobacterium species was isolated from two patients with crohn's disease. the organism is an acid-fast, mycobactin-dependent mycobacterium that has characteristics which do not conform to any of the presently recognized species. it belongs to the runyon group iii mycobacteria and is most closely related to mycobacterium paratuberculosis. animal inoculation revealed pathogenicity for mice when injected intravenously or intraperitoneally, but not for rats, guinea pigs, r ... | 1984 | 6499624 |
| possible role of mycobacteria in inflammatory bowel disease. ii. mycobacterial antibodies in crohn's disease. | an unclassified mycobacterium species has been isolated from two patients with crohn's disease (cd). antibodies to the unclassified mycobacteria cross-reacted with mycobacterium paratuberculosis. because of this cross-reactivity, an enzyme-linked immunosorbent assay (elisa) was used to examine the sera of inflammatory bowel disease (ibd) patients, both cd (n = 56), and ulcerative colitis (uc) (n = 34), for antibodies to m. paratuberculosis, mycobacterium kansasii, and mycobacterium tuberculosis. ... | 1984 | 6437775 |
| agar gel immunodiffusion test for diagnosis of clinical paratuberculosis in cattle. | during a 19-month period, the agar gel immunodiffusion (agid) test was used as an aid in differential diagnosis of paratuberculosis in 51 cattle with a history of chronic weight loss and/or chronic diarrhea. thirty-three cattle were agid test-positive for paratuberculosis. twenty-eight cattle (87.5%) yielded mycobacterium paratuberculosis from bacterial culture of feces. four cattle were confirmed as having paratuberculosis on necropsy. one had a negative fecal culture but was lost to follow-up. ... | 1984 | 6430850 |