Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| animal models of nonthyroidal disease. | rats bearing transplantable walker 256 carcinoma provide an opportunity to assess thyroid function and activity during an interval of time when the tumor has not affected growth rate. rats with tumor have decreased serum t4 and t3 concentration and decreased serum ft4 and ft3 as well. these changes are due to a decrease in binding of iodothyronines by the serum binding proteins, an increase in the fractional rate of t4 metabolism and a decrease in thyroidal secretion. the decrease in activity of ... | 1985 | 3884330 |
| an immunohistological approach to persistent lymphadenopathy and its relevance to aids. | recent evidence has shown that not only aids but also the majority of 'unexplained' persistent, generalized lymphadenopathy (pgl) are related to htlv-iii/lav infections. the early detection how these changes may proceed to aids then become a prime interest. eleven patients with pgl (10 homosexual males and one heterosexual haemophiliac) have been studied by immunohistology using monoclonal antibodies to dendritic reticulum cells of the germinal centre, t and b lymphocyte subsets, plasma cells an ... | 1985 | 3884195 |
| subset specificity of antilymphocyte antibodies in systemic lupus erythematosus. preferential reactivity with cells bearing the t4 and autologous erythrocyte receptor phenotypes. | the relative specificity of systemic lupus erythematosus antilymphocyte antibodies for t cell subsets bearing the okt4, okt8, or autologous erythrocyte rosette (a-rfc) markers was examined in complement-dependent microcytotoxicity assays and by indirect immunofluorescence. target cells included normal okt4+ or okt8+ t cell clones established from mitogen-activated blasts, resting or phytohemagglutinin-activated peripheral t cells highly enriched for okt4+ cells or okt8+ cells, and a-rfc+ and a-r ... | 1985 | 3884018 |
| [thyroid function and amiodarone. consequences on dysthyroidism testing]. | amiodarone induces a decrease in serum t3 whereas t4 and rt3 increase. an increase of the thyroid iodine content (tic) is observed in all patients at the exception of those who develop hypothyroidism under treatment. actually, no method are available to predict an induced thyroid toxicosis (itt) and there is no reason to perform systematically thyroid function tests except if past of the patient or clinical or morphological thyroid examinations suggest thyroid abnormality. in case of suspicion o ... | 1985 | 3883881 |
| effects of epidural anesthesia on catecholamines, renin activity, and vasopressin changes induced by tilt in elderly men. | mean arterial pressure, heart rate, plasma catecholamines, renin activity, and vasopressin changes induced by a 30-degree head-up tilt were studied before and during epidural anesthesia with bupivacaine in eight elderly patients (ages 58-82 yr). the tilt performed before epidural anesthesia did not modify mean arterial pressure, heart rate, plasma catecholamines, renin activity, and vasopressin at 5 and 15 min. during epidural anesthesia, the superior level of analgesia ranged from t4 to t10. ep ... | 1985 | 3883849 |
| mechanism of bromocriptine-induced hyperglycaemia. | results reveal that bromocriptine at a dose of 6 mg/kg. i.p. in mice caused a significant hyperglycaemic effect which was accompanied by a marked increase in liver glycogen. after adrenalectomy, the effect of bromocriptine on serum glucose level was reduced whereas its effect on liver glycogen content was abolished. in rats, administration of bromocriptine (17.5 mg/kg i.p.) induced a significant rise in serum glucose level which was associated with marked reduction in serum insulin activity and ... | 1985 | 3883091 |
| location of the adenylylation site in t4 rna ligase. | the purification of the enzyme t4 rna ligase is described from an escherichia coli strain, kr54, in which the rna ligase gene (g63) has been inserted into the plasmid pdr540 for inducible expression of g63 from the tac promoter. adenylylation of the purified enzyme with [14c]ratp followed by digestion with chymotrypsin yielded an adenylylated peptide, the identity of which was determined by fast-atom-bombardment mass spectrometric analysis. the results show that the amp residue is bound covalent ... | 1985 | 3882425 |
| effect of thyroidectomy and thyroxine on 2,3,7,8-tetrachlorodibenzo-p-dioxin-induced immunotoxicity. | radiothyroidectomy protected against 2,3,7,8-tetrachlorodibenzo-p-dioxin (tcdd)-induced immunotoxicity in rats as assessed by the spleen anti-srbc plaque-forming cell assay. thyroxine (t4) replacement therapy partially reversed the effects of thyroidectomy on t4 and triiodothyronine (t3) serum levels, body weight and immune function as well as restored tcdd-induced immunotoxicity. thus, hypothyroidism induced by tcdd exposure can be viewed as a protective response of the organism to reduce the i ... | 1985 | 3881642 |
| response of a phage modification factor to enhanced production of its target molecule. | escherichia coli cells with plasmids bearing the vals gene were constructed from the clark-carbon collection. their increased valyl-trna synthetase (ec 6.1.1.9) activity was not matched by commensurate production of a modifying peptide during t4 infection. thus, phage vs gene expression is set to modify the normal amount of valyl-trna synthetase activity. | 1985 | 3881597 |
| effects of dietary energy supply on serum thyroxine, tri-iodothyronine and insulin concentrations in young horses. | the effects of meal ingestion on the circulating concentrations of the growth-regulating hormones thyroxine (t4), tri-iodothyronine (t3) and insulin were examined in weanling thoroughbreds fed 70% (diet a), 100% (diet b) or 130% (diet c) of their energy and protein requirements. peak insulin concentrations occurred 1, 2 and 3 h after the ingestion of diets c, b and a respectively. increases in plasma glucose concentrations preceded the increases in serum insulin concentrations. serum t4 concentr ... | 1985 | 3881553 |
| pulmonary sarcoidosis: flow cytometry measurement of lung t cell activation. | lung t cell activation is considered a major factor in the pathogenesis of pulmonary sarcoidosis. our study was designed to investigate several parameters of t cell activation among blood and alveolar cell populations, including expression of hla-dr or mlr antigens, increased cell size, and presence of dividing cells. blood sampling and bronchoalveolar lavages were performed in 20 patients with pulmonary sarcoidosis. cell populations were analyzed by flow cytometry using immunofluorescence label ... | 1985 | 3881544 |
| isolation of altered reca polypeptides and interaction with atp and dna. | in this paper we describe the partial proteolytic digestion of reca proteins from escherichia coli and proteus mirabilis and the production and isolation of truncated reca polypeptides. a proteolytic fragment of the p. mirabilis reca protein bound single-strand dna and atp normally but has altered duplex dna binding properties. this protein was shown to initiate but not complete dna strand transfer from a dna duplex to a complementary single strand. the product of the e. coli reca1 allele bound ... | 1985 | 3881430 |
| the t4 mot protein functions as part of a pre-replicative dna-protein complex. | middle-mode rna synthesis in t4-infected cells takes place before replication of phage dna commences. what distinguishes it from early-mode rna synthesis is that initiation of middle rna depends on t4-coded proteins, in particular on the mot gene product. mot protein is localized in a dna-protein complex which forms during the first few minutes of infection. all of the cell's mot protein is bound in this complex, and it continues to be bound long after the synthesis of mot protein has stopped. w ... | 1985 | 3880747 |
| t-lymphocyte-subsets in endocrine exophthalmos. | peripheral t-lymphocyte subsets were analyzed with monolateral antibodies in 40 patients with graves' ophthalmopathy. the 20 patients with untreated hyperthyroid exophthalmos showed a slight statistically not significant decrease in the percentage of total t-lymphocytes and a statistically significant decrease with percentage of the ok t8 cells. no significant changes were observed in the percentage of ok t3 and ok t4 and ok t8 cells in patients with euthyroid exophthalmos under the drug treatme ... | 1985 | 3879963 |
| [acute negative chronotropic effect of l-thyroxine on the everted sinus-atrium preparation of the bullfrog]. | an application of l-thyroxine (t4) to bullfrog everted sinus-atrium preparations showed an acute negative chronotropic effect instead of a positive one in the chronic phase. the acute effect was dose dependent (7-70 microm) and the maximal negative chronotropic effect (about + 20% interval prolongation) was observed at 40 microm t4. the acute t4 effect on the cardiac interval consisted of two stages: an initial peak (about 5 min) accompanied by a transient increase in contractility, and a delaye ... | 1985 | 3879500 |
| t lymphocyte subpopulations in progressive systemic sclerosis defined by monoclonal antibodies. | twenty two patients with progressive systemic sclerosis were studied by monoclonal antibodies to detect okt4 and okt8 positive cells. the absolute number of okt4 and okt8 cells was not altered as compared to control values. the ratio of t4/t8 cells slightly increased without statistical significance. the number of e-rosette forming t-cells was reduced in patients with progressive systemic sclerosis. six months later 11 patients were reinvestigated, and similar results were obtained for the t-cel ... | 1985 | 3879347 |
| human pregnancy serum inhibits proliferation of t8-depleted cells and their interleukin-2 synthesis in mixed lymphocyte cultures. | t8-depleted and unfractionated t lymphocytes allogeneically stimulated and cultured in the presence of pregnancy sera exhibit an inhibition of cellular proliferation and interleukin-2 synthesis, respectively. unfractionated t cells show a decrease in their cytotoxicity in the presence of these sera. the inhibition of cytotoxicity could be due to the deficit of il-2 observed since if exogenous il-2 is added to the cultures t4/leu3a-depleted allogeneically stimulated cells reach the same degree of ... | 1985 | 3879273 |
| acute changes of iodothyronine excretion by bile after a single and repeated administration of dexamethasone in rats. | bile was collected from cannulated bile duct under pentobarbiturate anesthesia and the excretion of several iodothyronines was estimated with the aid of radioimmunoassay as described previously. the excretion of triiodothyronine (t3) was significantly decreased between 4 and 6 h after a single injection of 20 and 40 mg kg-1 dexamethasone (dex), while no changes in the excretion of thyroxine (t4) and reverse triiodothyronine (rt3) were found. in another experiment a decrease of t3 excretion was o ... | 1985 | 3878778 |
| effect of cyclophosphamide on thyroid gland in mice and guinea pig. | male balb-/c mice and guinea pigs were given cyclophosphamide (cy)-either a single dose of 20 or 200 mg kg-1 or multiple doses of 2 or 20 mg kg-1 daily for 10 days. on the 1st, 3rd, 7th and 15th day after the last dose the serum levels of tsh, t4, t3 and rt3 were measured by ria. the thyroids were processed for light and transmission electron microscopy. both acute and chronic administration of cy resulted in a significant and prolonged decrease of serum t4 levels in mice and guinea pigs. in con ... | 1985 | 3878777 |
| pokeweed mitogen and staphylococcus aureus cowan i induced immunoglobulin a synthesis by lymphocytes of iga deficient blood donors. | in vitro iga synthesis induced by pokeweed mitogen (pwm), staphylococcus aureus cowan i (sta), and combinations of sta and pwm (sta/pwm) was studied in lymphocytes of iga deficient (sigad) blood donors. cultures of t-depleted (non-t) cells with autologous or allogeneic control t, irradiated t (t), or t4 cells suggested abnormalities in non-t cell fractions in most (12/22) sigad donors. t cell abnormalities in themselves, detectable in six donors, did not appear to account for the failure of iga ... | 1985 | 3878748 |
| cellular immunity against salmonella typhi after live oral vaccine. | seventeen adult volunteers were vaccinated orally with the live attenuated salmonella typhi mutant strain ty21a. their peripheral blood mononuclear cells were tested at different times after vaccination for direct cell-mediated activity against bacteria, employing a simple short-term in vitro assay. it was observed that 16/17 of the vaccinated subjects acquired the capacity to express specific cellular immunity against s. typhi which lasted from 15 days to at least 3 years. the effector cell of ... | 1985 | 3878744 |
| langerhans cells in sle skin. a role in lymphocyte migration and activation in situ. | langerhans cells in sle skin and mucosa were studied by using monoclonal anti-t6 and anti-ia antibodies and avidin-biotin-peroxidase complex staining. langerhans cells were present in cell infiltrates of natural sle skin lesions. in developing early skin lesions induced with test antigen, langerhans cells were the predominant inflammatory cells, suggesting that migration of langerhans cells into the inflammatory site in fact precedes lymphocyte migration. these findings agree with the hypothesis ... | 1985 | 3878583 |
| [proteins of the basal plate of bacteriophage t4 participating in the contractile impulse]. | reversible linking of proteins with cu2+ and ni2+ ions was used to study the topography of the structural proteins of bacteriophage t4 basal plate. gene products (gp) 9 and 10 were found to directly contact the proximal part of long fibrils (gp34). gp27, gp54 and gp5 interact with the lower disk of the contractive sheath (gp18), while gp48 and gp54 are in contact with the core (gp19). the proteins of the sheath (gp18) and the core (gp19) were found to have contact over the whole tail length. | 1985 | 3878456 |
| longitudinal study of htlv-iii-infected chimpanzees by lymphocyte subpopulation analysis. | following inoculation with plasma from human patients with aids, two chimpanzees demonstrated specific antihuman t-cell leukemia virus type 3 (htlv-iii) antibodies. one of the two chimpanzees also developed massive lymphadenopathy that persisted for 32 weeks and demonstrated a concurrent and more frequent depression of total t cells (t3) and t helper cells (t4) with a decrease in the ratios of t4 to t suppressor cells (t8). these results indicate that chimpanzees demonstrate a range of t-cell su ... | 1985 | 3878410 |
| anti-thymocyte globulin and prednisone immunotherapy of recent onset type 1 diabetes mellitus. | increasing evidence that type 1 (insulin dependent) diabetes mellitus is an autoimmune disease, together with successful cure/prevention in animal models of this disease (e.g. bb/w rat) has led to several trials of immunotherapy in recent onset type 1 diabetes of man. in this communication we report our experience with short courses of prednisone and antithymocyte globulin (atgam) plus prednisone. prednisone characteristically suppressed ia positive t lymphocytes into the normal range, but had n ... | 1985 | 3878262 |
| acute leukemia of hybrid phenotype: t lymphoid and myelomonocytic markers. | several unique phenotypical and functional characteristics were found together in a patient with acute poorly differentiated leukemia. the blast cells showed an unusual t-cell phenotype, forming spontaneous rosettes with sheep red blood cells and expressing the t11 antigen, but were negative for the other immature or mature t markers tested--leu-1, 3a1, t3, t4, t8, t9, t10, t6, and tdt--and reacted with the monoclonal antibody okm1 expressed by myeloid lineage cells and which is also present in ... | 1985 | 3878245 |
| immunodynamics of minimal change nephrotic syndrome in adults t and b lymphocyte subsets and serum immunoglobulin levels. | thirty-two adult patients with minimal change nephrotic syndrome (mcns) were studied in order to clarify the characteristics of the immune system in mcns and their relation to clinical activity. in the active phase (n = 17), serum immunoglobulin (ig) m and e levels, b lymphocytes (surface ig-positive cells) and their subsets, surface igg, igm and ige positive cells; b gamma, b mu and b epsilon, were increased, whereas the serum igg level and okt3-reactive cells, peripheral t lymphocytes; t3, wer ... | 1985 | 3878244 |
| role of the gene 17 protein of bacteriophage t4. | head-related particles of bacteriophage t4 were examined by using heat leakage scanning calorimetry. the 17- particles showed two endothermic peaks on thermograms during their thermal transition process, while 49- particles gave only a single sharp endothermic peak. thermograms of 17- particles treated with 23- defective lysate were different from those of nontreated 17- particles, and closely resembled thermograms of 49- particles. the 31- defective lysate was also capable of converting thermal ... | 1985 | 3878031 |
| t lymphocyte subpopulations in high-risk infants: influence of age and blood transfusions. | methodology was established to analyze t lymphocytes and t cell subsets with monoclonal antibodies on microsamples of blood obtained by heel puncture in infants. results in 39 high-risk infants indicated that during the early neonatal period they had a higher percentage of t4-bearing cells and a lower percentage of t8 antigen-positive cells as compared with adults. these values progressively approached adult values, and at 3 to 7 months the t cell subsets and the t4/t8 ratios were within the adu ... | 1985 | 3877911 |
| phenotypic and functional heterogeneity of human peripheral blood t lymphocytes producing colony stimulating factor. a clonal and precursor frequency analysis. | in this report we describe the precursor frequency and the subset distribution of peripheral blood human t cells producing lymphokine(s) acting on the proliferation and differentiation of bone marrow precursors of the granulocyte/macrophage series, as assessed in a liquid microculture assay. because the sensitivity of this system was similar to that of the classic colony formation assay in semi-solid (methylcellulose) medium, it is likely that the lymphokine activity measured in this assay corre ... | 1985 | 3877760 |
| persistent lymphadenopathy associated with hypertransfusion in sickle-cell disease. | we report the results of an immunologic evaluation of two hypertransfused (ht) patients with sickle-cell disease (scd) who have developed persistent generalized lymphadenopathy. in order to interpret the results of this evaluation, we studied seven other ht patients with scd and seven nonhypertransfused patients with scd. patients with scd had decreased percentages of t-lymphocytes to include both helper and suppressor subsets in their peripheral blood. these decreases resulted in t helper/suppr ... | 1985 | 3877748 |
| cutaneous pre-b leukaemia cells with a t-lymphocyte antigen (t4). | a patient with acute lymphocytic leukaemia (all) is described in whom the cells originated in the pre-b cell lineage but showed an aberrant expression of t4 antigen. a b-cell origin of the leukaemic cells was indicated by reactivity for several monoclonal antibodies specific for the b-cell lineage and by cytoplasmic staining with fitc-labelled anti human mu chains. | 1985 | 3877340 |
| cytolytic activity of t lymphocytes isolated from ovarian carcinoma ascitic fluid. analysis at the population and clonal level. | t lymphocytes were isolated from ascitic fluid of three patients with ovarian carcinoma at iii-iv stage. surface markers analysis of such purified t cells revealed that t8+ cells were well represented among ascitic t lymphocytes (from 35 to 56%). low percentages of activated t cells, as indicated by hla-dr and tac (interleukin-2 receptor) positivity, were also present. however, fresh ascitic t lymphocytes failed to lyse autologous tumor target cells in a 4-h 51cr release assay. furthermore, by a ... | 1985 | 3877335 |
| bacteriophage t4 rega protein. purification of a translational repressor. | the bacteriophage t4 rega protein translationally regulates its own synthesis and the synthesis of several other t4 early proteins. in order to study the mechanism of translational regulation, we have purified the rega protein. initially a mutant protein, incapable of autogenous repression, was placed under lambda pl transcriptional control and amplified to approximately 10% of total cell protein. the membrane-associated mutant protein was extracted with organic solvent mixtures and purified by ... | 1985 | 3877050 |
| neonatal hyperthyroidism alters submandibular gland epidermal growth factor response to thyroxine in the adult mouse. | neonatal hyperthyroidism (nh) in the rat is associated with permanent reductions in serum thyroxine (t4), triiodothyronine (t3), and thyroid-stimulating hormone (tsh) concentrations in the adult, changes suggestive of a hypothyroid state. in the adult nh rat, the thyrotroph appears to be more sensitive to the feedback effects of thyroid hormones. to determine whether thyroid hormone sensitive tissues retain their responsiveness to thyroid hormones, the long-term effects of nh on mouse submandibu ... | 1985 | 3876870 |
| the immunohistology of follicle lysis in lymph node biopsies from homosexual men. | follicle lysis is a characteristic alteration of b cell follicles described recently in lymph node biopsies from homosexual men. it consists of disruption of germinal centers by aggregates of small mature lymphocytes variably associated with erythrocyte extravasation. we studied the immunohistology of follicle lysis identified in lymph node biopsies from 11 homosexual men. the results indicate that follicle lysis has two principal immunohistologic features: (1) intrafollicular aggregates of smal ... | 1985 | 3876855 |
| phorbol ester induces a differential effect on the effector function of human allospecific cytotoxic t lymphocyte and natural killer clones. | six-day allosensitized human peripheral blood lymphocytes treated for 12-18 hr with phorbol 12-myristate 13-acetate (pma) were found to lose the capacity to kill the specific target in a standard cell-mediated lympholysis (cml) assay, but they were still effective in their ability to kill the tumor cell line k562. we investigated which antigens on the cell membrane involved in alloimmune recognition might be modified by pma, since it was found that in a lectin-dependent cml assay, the lytic mech ... | 1985 | 3876559 |
| anticlonotypic monoclonal antibodies induce proliferation of clonotype-positive t cells in peripheral blood human t lymphocytes. evidence for a phenotypic (t4/t8) heterogeneity of the clonotype-positive proliferating cells. | three previously selected monoclonal antibodies (mab) directed against the clonotypic structure of a variant (termed ja3) of the interleukin 2 (il-2)-producing jurkat leukemia cell line (anti-jti1-3 mab) were found to induce an adherent cell-dependent proliferation of peripheral blood t cells in 20 different donors. unlike the early cell proliferation induced by anti-t3 mab, anti-jti mab-induced proliferation was detectable at day 5-6 of culture and reached peak levels at day 7-9. less than 1% j ... | 1985 | 3876404 |
| t8 antigen density on peripheral blood lymphocytes remains unchanged during exacerbations of multiple sclerosis. | fluorescence-activated cell sorter (facs) analysis was used to quantitate the antigen density of the lymphocyte markers t3, t4, t8, and leu 7 on the surface of peripheral blood mononuclear cells (mnc) from exacerbating/remitting multiple sclerosis (ms) patients. serial studies showed that the mean fluoresce intensity for all the markers studied did not change significantly during exacerbations. there was a tendency for a generalized decline in t-cells, evidenced by a drop in both t8+- and t4+-ce ... | 1985 | 3876353 |
| quantitative and functional abnormalities of total t lymphocytes in relatives of patients with hodgkin's disease. | seven patients, long-term survivors of hodgkin's disease, and 24 of their relatives (parents, siblings and children), together with normal controls were studied for percentages, absolute counts and mitogen-proliferative responses by means of monoclonal antibodies, e rosette technique and in vitro cultures with pha, cona and pwm. the aim of the study was to ascertain whether the impaired cell-mediated immunity of hodgkin's patients was also present in relatives in order to elucidate the still deb ... | 1985 | 3876218 |
| thyroid hormones in rats during long-term cold exposure and hypometabolic effect of reverse triiodothyronine on adrenaline induced thermogenesis. | one-day cold exposure to 5 degrees c induced temporal increase of reverse-triiodothyronine (rt3), triiodothyronine (t3) and thyroxine (t4) concentrations in the blood of rats (by 151%, 91% and 107%, respectively). the levels of all thyroid hormones remain elevated for at least 10 days of cold exposure. the injection of rt3 (330 micrograms kg-1 s.c.) inhibited the resting and adrenaline stimulated metabolism 1-3 days after application independently from the thyroid status of the animals. since th ... | 1985 | 3876207 |
| acquisition of urine, kidney and submandibular gland epidermal growth factor responsiveness to thyroxine administration in neonatal mice. | thyroxine (t4) administration to newborn mice on days 0-6 produced no measurable changes of submandibular gland (smg) or kidney epidermal growth factor (egf) concentrations on day 7, compared with vehicle-treated control pups. by contrast, this regimen caused a large increase in urine egf levels. the effects of three t4 injection regimens (on days 0-6, 7-14 and 0-14) were then studied on day 15. the 0-14 and 7-14 day-regimens elicited large increments in urine, smg and kidney egf concentration, ... | 1985 | 3875958 |
| involvement of t44 molecules in an antigen-independent pathway of t cell activation. analysis of the correlations to the t cell antigen-receptor complex. | prior studies indicate that the 9.3 monoclonal antibody (mab) which defines a 44 kd t lineage-specific glycoprotein (t44) enhances the proliferative response of peripheral blood t lymphocytes to phytohemagglutinin (pha) or allogeneic cells. the t44 molecule was expressed in both resting and activated t lymphocytes and in a subset of thymocytes, as assessed by indirect immunofluorescence and flow cytofluorometry. in view of the potential importance of t44 in t cell activation, we investigated the ... | 1985 | 3875683 |
| clonal analysis of t lymphocytes isolated from ovarian carcinoma ascitic fluid. phenotypic and functional characterization of t-cell clones capable of lysing autologous carcinoma cells. | t lymphocytes isolated from ascitic fluid of patients with stage iii-iv ovarian carcinoma were cloned by means of a microculture system that allows cloning of virtually all peripheral blood human t lymphocytes. under these experimental conditions, 15% to 42% of ascitic t cells gave rise to clonal progenies that were analyzed for different functional capabilities. of the clones obtained, 36%-70% had cytolytic activity in a pha-dependent assay (using p815 as target cells) that allowed detection of ... | 1985 | 3875569 |
| phenotypic characteristics of human natural killer cells. | surface antigen analysis of human natural killer (nk) cells using anti-lymphocyte monoclonal antibodies identifies nk cells as a discrete cell type (average 15% of peripheral blood lymphocytes) distinct from t, b, and myelomonocytic cells. virtually all nk cells bear low-affinity fc receptors for igg, as detected by antibody b73.1 and n901 antigen; 80-90% of nk cells express the e receptor (okt11), the c3bi receptor (okm1), and the t10 antigen; 30-50% are t8 (+), and 30-70% are leu7 (+). this ph ... | 1985 | 3875369 |
| mitogen responses and t4/t8 ratios in asymptomatic hemophiliac patients. | lymphocyte responses to mitogen stimulation were studied in healthy hemophiliac patients. to study kinetics and dose response, three different concentrations of the mitogens phytohemagglutinin (pha) and pokeweed mitogen (pwm) were used, and proliferation was measured on each of 6 days. patients with t4/t8 ratios of less than 1.00 showed decreased proliferation compared to normal controls and to hemophiliac patients with ratios greater than 1.00. these findings were more pronounced with pwm than ... | 1985 | 3875171 |
| immunohistological analysis of lung tissue from patients with cryptogenic fibrosing alveolitis suggesting local expression of immune hypersensitivity. | immunohistological analysis using monoclonal antibodies in conjunction with histochemical techniques has been applied to lung biopsy material from patients with cryptogenic fibrosing alveolitis. subsets of lymphoid and non-lymphoid cells have been identified in situ. this analysis showed that the inflammatory cells present were predominantly mononuclear. most of the lymphoid cells were b lymphocytes, organised into follicles with occasional germinal centre formation. igm was the major class of i ... | 1985 | 3875162 |
| recurrent diffuse pulmonary hemorrhage with minor kidney lesions. | this case report concerns a 14-year-old boy with a 3 month history of dyspnea and iron deficiency anemia. on admission he had hemoptysis and bilateral pulmonary shadows. transbronchial lung biopsies showed linear deposits of igg and c3 in the alveolar basement membrane, but no anti-gbm antibodies were observed in serum or kidney biopsy. the ratio of the t cell subpopulations t4/t8 in peripheral blood was in the early stage, 5 and, thus, elevated. the patient was given prednisolone 1 to 0.25 mg/k ... | 1985 | 3874785 |
| a case of graves' disease with anti-triiodothyronine antibodies. | a case of graves' disease with high serum thyroxine (t4) and low triiodothyronine (t3) levels which was therefore initially diagnosed as a t4-thyrotoxicosis is reported. examination of the serum from the patient showed the presence of unusual protein which bound t3. it was later confirmed as igg class anti-t3 antibodies. in addition to treatment with methylmercaptoimidazole (mmi), the patient was treated with prednisolone for 30 days (total amount 500 mg). titers of anti-t3 antibodies in the ser ... | 1985 | 3874769 |
| lpf-induced t cell colony formation: effect of pma and interleukin-2, and surface marker analysis. | we demonstrated that a primary exposure to the lymphocytosis promoting factor (lpf) of bordetella pertussis-induced t cell colony formation. colony formation was observed when mononuclear cells (mnc) were cultured at concentrations of more than 1 x 10(6)/ml, and reached a peak on day 8. however, the number of colonies generated with lpf was about one-third induced with phytohaemagglutinin (pha). removal of monocytes from mnc or t cells resulted in the failure of colony formation, but colony grow ... | 1985 | 3874728 |
| evaluation of natural killer cell activity in patients with persistent generalized lymphadenopathy and acquired immunodeficiency syndrome. | natural killer (nk) cell activity was quantitated using 51cr release from the human erythroleukemia cell line k562 in 39 heterosexual males, 60 asymptomatic homosexuals, 39 patients with persistent generalized lymphadenopathy (pgl), and 16 patients with acquired immunodeficiency syndrome (aids). pgl and aids patients showed a slight decrease in nk cell activity compared to control groups. absolute numbers of leu 11a-positive cells were decreased in pgl and aids patients, and this decrease correl ... | 1985 | 3874030 |
| abnormal in vitro proliferation and differentiation of t colony forming cells in aids patients and clinically normal male homosexuals. | t cell colonies were generated from the peripheral blood and bone marrow of 11 patients with acquired immune deficiency syndrome (aids), 17 normal male and female heterosexuals and seven clinically normal male homosexuals. mononuclear cells were cultured in methylcellulose both in the absence and presence of interleukin-2 (il-2) containing conditioned medium. clinically normal homosexuals showed a low number of t4+ (p less than 0.01) but not t8+ cells. the number of t cell colony forming cells ( ... | 1985 | 3874021 |
| immunoregulation of the in vitro anti-hbs antibody synthesis in chronic hbsag carriers and in recently boosted anti-hepatitis b vaccine recipients. | we report a study on immunoregulation of in vitro antibody to hepatitis b surface antigen (anti-hbs) synthesis induced by pokeweed mitogen (pwm) from peripheral blood mononuclear cells (pbmc) in chronic hepatitis b surface antigen (hbsag) carriers and in 'high responders', (anti-hbs ria ratio greater than or equal to 20 in serum), recently boosted with anti-hepatitis b vaccine. anti-hbs was detected in 11 days pbmc supernatants (sn) from 24 out of 36 'high responders', but in none from 31 chroni ... | 1985 | 3874020 |
| t lymphocytes in infectious mononucleosis. ii. response in vitro to interleukin-2 and establishment of t cell lines. | the addition of 20% interleukin-2 (il-2) significantly reduced the percentage of t lymphocytes dying in vitro after being isolated from the peripheral blood of acute infectious mononucleosis (im) patients. moreover, the immediate addition of 20% il-2 to freshly isolated blood allowed im t cell lines to be readily established from the peripheral blood of acute im patients. characterization of seven of these im t cell lines showed them to be t3+, t11+, t4-, t9- and generally t10-. over half of the ... | 1985 | 3874018 |
| evaluation of t cell subsets in behçet's syndrome using anti-t cell monoclonal antibodies. | several immunological abnormalities have been described in behçet's syndrome, the multisystem disease characterized by the triad of relapsing iridocyclitis with recurrent oral and genital ulcerations. in the present study we have evaluated t cell subsets in the peripheral blood of patients suffering from behçet's syndrome, using a panel of anti-t cell monoclonal antibodies. when compared with normal subjects, patients with behçet's syndrome show a discrepancy between the number of t3+ cells and ... | 1985 | 3874016 |
| t4 lymphopenia in patients with active pulmonary tuberculosis. | the numbers of cells bearing the t3 (pan-t cell), the t4 (putative helper/inducer cells), the t8 (putative suppressor/cytotoxic cells) and b cell phenotypic markers were counted in venous blood samples from 26 newly diagnosed pulmonary tuberculosis patients and 29 healthy controls from east java. the absolute t cell count was lower in the patients and t4 cells were fewer in patients (mean 748/mm3) than in controls (mean 1,043/mm3), but there were no significant differences in total t8 cell and b ... | 1985 | 3874015 |
| effects of recombinant leukocyte interferon (rifn-alpha a) on tumour growth and immune responses in patients with metastatic melanoma. | studies were initiated to assess the response of patients with disseminated melanoma to recombinant alpha interferon (rifn-alpha a) and to monitor effects of rifn-alpha a on several tests of immune function. twenty patients were treated with rifn-alpha a given by i.m. injection in escalating doses from 15 to 50 x 10(6) um-2. the responses of two patients were considered unevaluable. of the remainder there was complete remission of tumour in two and stable disease in two. subsequent progression o ... | 1985 | 3873953 |
| somatic gene mutations in vivo as indicated by the 6-thioguanine-resistant t-lymphocytes in human blood. | the clonal and the autoradiographic assays for 6-thioguanine-resistant (tgr) t-lymphocytes (t-lys) in human blood are reviewed. studies of tgr colonies recovered from clonal assays show that the mutant t-lys (i) are either helper (t4) or suppressor (t8) cells, (ii) possess stable tgr phenotypes, (iii) are deficient in hypoxanthine guanine phosphoribosyltransferase (hprt), and (iv) have structural alterations in the hprt gene. tgr t-ly mutant frequencies (mfs) determined by clonal assays are of t ... | 1985 | 3873612 |
| hydrodynamic studies of a dna-protein complex. dimensions of the complex of single-stranded 145 base dna with gene 32 protein of phage t4 deduced from quasi-elastic light scattering. | the translational diffusion coefficient of the saturated complex of single-stranded 145 base dna and the helix-destabilizing protein of phage t4, gp32, can be measured at equilibrium by means of quasi-elastic light scattering. if the complex is considered as a rigid rod one can estimate its dimensions by combining the translational diffusion coefficient with earlier data on rotational diffusion. it was found that the average base-base distance of the 145 base dna in the complex is between 4.3 an ... | 1985 | 3873355 |
| lymphocyte subpopulations in graves' ophthalmopathy. | we assessed the percentages of t-lymphocyte subsets, b lymphocytes, monocytes, and granulocytes in the mononuclear cell preparations of euthyroid patients with minimal and severe graves' ophthalmopathy. patients with active werner class 4-5 and class 6 ophthalmopathy had statistically significant elevations of suppressor/cytotoxic t8+ lymphocytes. during successful corticosteroid therapy, the number of t8+ lymphocytes returned to the normal range. mirroring these results, the t4/t8 ratio was ini ... | 1985 | 3873236 |
| implantation site in complete molar pregnancy: a study of immunologically competent cells with monoclonal antibodies. | the nature and intensity of inflammatory cellular infiltrate in the implantation sites of 10 complete molar pregnancies were evaluated by immunohistochemical staining of frozen tissue sections with monoclonal antibodies. as compared to the implantation site in normal pregnancy, there was an increase in the number of infiltrating inflammatory cells in the molar implantation site. most of the inflammatory cells were t cells with predominance of t4+ (leu-3a+) cells over t8+ cells. inflammatory cell ... | 1985 | 3873176 |
| enhancement of mitogen response and surface marker analysis of lymphocytes from young and old donors after preliminary incubation in vitro. | we have examined the detailed kinetics of pha induced proliferation of freshly isolated mononuclear cells from young and old human donors. our studies confirm that donors over the age of 60 years may have a decreased number of pha responsive cells and that these cells have a significantly diminished rate of entry into the first cell cycle. age of the donor does not affect the time at which significant numbers of cells appear in first s-phase, the duration of first s-phase, the doubling time in e ... | 1985 | 3872977 |
| phorbol ester-induced differentiation of chronic b lymphocytic leukaemia cells--regulatory impact of autologous and allogeneic accessory cells. | phorbol ester (tpa) induction of chronic lymphocytic leukaemia (cll) cells can be used as a model system for the study of human b cell differentiation. we have analysed the role of accessory cells and the correlation to target cell surface ig phenotype in tpa-induced morphological and functional differentiation of 12 cll populations. a more than three-fold increase in secreted igm was seen in 10 of 12 cases, with the strongest responses in patients having monoclonal serum igm. cll populations ne ... | 1985 | 3872753 |
| disproportionate lymphoid cell subsets in thalassaemia major: the relative contributions of transfusion and splenectomy. | the relative proportions of t-cell (okt3-positive, okt4-positive and okt8-positive) and b-cell (sig-positive) populations in peripheral blood obtained from 29 chronically transfused patients with beta-thalassaemia major were compared with those of 17 healthy controls. changes attributable to blood transfusion and/or splenectomy are described. the percentage of okt8-positive (t-suppressor) cells found in the thalassaemic patients increased linearly (p less than 0.001) with the number of units tra ... | 1985 | 3872679 |
| epidermal growth factor in mouse urine: non-blood origin, and increase by sialoadenectomy and t4 therapy. | to study the origin and regulation of urinary epidermal growth factor (u-egf), we measured egf in adult female mouse plasma, urine and kidney tissue. we also studied the effects of excision of the submandibular salivary gland (smg) and, after smg excision and sham operation, 10-day therapies with t4 (0.4 microgram/g daily), testosterone propionate (tp, 12.5 micrograms/g every 48 h), and smg extract (25 micrograms egf daily). we measured egf with a specific liquid phase radioimmunoassay. the mean ... | 1985 | 3872550 |
| cloning, nucleotide sequence, and overexpression of the bacteriophage t4 rega gene. | the bacteriophage t4 rega gene codes for a regulatory protein that controls the expression of a number of t4 early genes, apparently at the level of translation. restriction fragments containing the rega structural gene have been cloned into phage m13, and the nucleotide sequence has been determined. translation of the dna sequence predicted that rega protein contains 122 amino acids, with a mr of 14,620. a dna fragment carrying 85% of the coding sequence of rega has been cloned into the phage l ... | 1985 | 3872458 |
| epidermal growth factor in mouse ocular tissue: effects of thyroxine and exogenous epidermal growth factor. | using a specific and sensitive epidermal growth factor (egf) radioimmunoassay, we identified radioimmunoassayable egf both in developing and adult mouse ocular tissues. in neonatal animals ocular egf concentrations increase during the first 4-9 days and then decline between 9 and 15 days. thyroxine (t4) administration (0.4 micrograms/g body weight/day from day 0) increased local egf concentrations in eye and skin of 7-day-old neonatal mouse pups. however, this treatment did not affect submandibu ... | 1985 | 3872442 |
| effect of chemotherapy on t-lymphocyte subsets in b-cell proliferative disorders. | the t-cell subset distribution and the activity markers of 41 patients with multiple myeloma, waldenström's macroglobulinaemia and benign monoclonal gammopathy were repeatedly analysed using monoclonal antibodies (t3, t4, t8, anti-tac, anti dr) and rosetting techniques. in myeloma and macroglobulinaemia the relative and absolute numbers of t4+ cells were diminished while the absolute number of t8+ cells was not decreased. no significant difference between stage i and iii of the myeloma disease w ... | 1985 | 3872145 |
| in vitro expansion and analysis of cloned cytotoxic t cells derived from patients with chronic t gamma lymphoproliferative disorders. | patients with t gamma lymphocytosis are a heterogeneous group, clearly distinguishable from other patients with chronic t lymphoproliferative disorders and usually without proven malignancy. we have attempted in vitro cloning of lymphocytes from three patients with an expansion of phenotypically and functionally different types of t gamma cells. one had t3+ b73.1+ t4 t8+ okm1+ t gamma cells exerting antibody-dependent cellular cytotoxicity (adcc) and natural killer (nk) cell cytotoxicity; anothe ... | 1985 | 3872139 |
| a longitudinal evaluation of thyroid function in critically ill surgical patients. | thyroid hormone alterations (known as the "sick-euthyroid syndrome") are common following major surgery, but the time course for appearance and recovery from these alterations has not previously been longitudinally studied in a large group of surgical patients. the authors prospectively studied 59 patients undergoing major surgery (coronary artery bypass grafting, pneumonectomy, or subtotal colectomy). compared with preoperative values, the mean serum t4, t3, free t3, and tsh concentrations decr ... | 1985 | 3872103 |
| correlation between immunologic function and clinical subpopulations of patients with the acquired immune deficiency syndrome. | the present study was designed to determine whether there was a significant correlation between the clinical presentation of patients with aids or aids-related illnesses and the degree of their underlying immunologic abnormalities. in 17 patients who presented with opportunistic infections, the mean number of t4 lymphocytes was 34/mm3 and the mean proliferative response to phytohemagglutinin 26,000 cpm; in 12 patients who presented with kaposi's sarcoma alone, the mean number of t4 cells was 231 ... | 1985 | 3872068 |
| human t-cell clones from autoimmune thyroid glands: specific recognition of autologous thyroid cells. | the thyroid glands of patients with autoimmune diseases such as graves' disease and certain forms of goiter contain infiltrating activated t lymphocytes and, unlike cells of normal glands, the epithelial follicular cells strongly express histocompatibility antigens of the hla-dr type. in a study of such autoimmune disorders, the infiltrating t cells from the thyroid glands of two patients with graves' disease were cloned in mitogen-free interleukin-2 (t-cell growth factor). the clones were expan ... | 1985 | 3871967 |
| [hla and myasthenia. subdivision in 3 categories]. | genetic susceptibility (hla types), clinical and pathological findings, amount of acetylcholine receptor antibodies and t lymphocyte subpopulations were studied in 63 patients with myasthenia gravis (mg). the frequency of hla-dr5 was increased among patients (0.50 versus 0.23 in controls, pc less than 0.01, relative risk 3.3) and that of hla-dr3 previously described as associated with mg was slightly increased (0.31 versus 0.20 in controls). the relative frequencies of two t cell subpopulations ... | 1985 | 3871932 |
| heterogeneity of human natural killer cells. | the cell surface antigens and specificity of cultures of human natural killer (nk) cells, propagated in the presence of interleukin 2, were analyzed at the single-cell level. with the use of a micropipette isolation procedure, clones were initiated from cells of defined phenotype. the cell surface markers on the resultant clones were not stable and often diverged from those on the cells selected for initiation. clones manifesting nk activity had low expression of t4 and t8 and most had some expr ... | 1985 | 3871873 |
| agar human t cell colony growth promoted by a b + null cell-derived lymphokine distinct from il 2. | human t cell agar colonies can be grown under pha stimulation from either mature t cells or their e rosette-negative (e-), okt3- peripheral blood and bone marrow precursors. colonies comprise a majority of mature e+, okt3+ cells and a minor (5 to 10%) population of immature e-, t3-, t8-, t4-, dr+, t10+, rfb1+ cells, which upon replating in subculture, can generate secondary colonies of okt3+, e+, okt4+, okt8+ cells. secondary colony formation can serve as a test for growth requirement of colony ... | 1985 | 3871813 |
| frequency and surface phenotype of human t lymphocytes producing interleukin 2. analysis by limiting dilution and cell cloning. | in this study we have determined the frequency and distribution of interleukin 2 (il2)-producing cells and their precursors (il2-p) in the two major subsets of human t cells. the two subsets were identified on the basis of their reactivity (or lack thereof) with anti-t4 or anti-t8 monoclonal antibodies. t4+t8- and t4-t8+ cells were first isolated from peripheral blood t cell populations by positive or negative selection using the fluorescence-activated cell sorter, and then analyzed for total il ... | 1985 | 3871697 |
| the effects of hyperthyroidism on experimental autoimmune thyroiditis in the rat. | the effects of hyperthyroidism on experimental autoimmune thyroiditis were investigated in the rat. rats were given t4 twice daily by sc injection in amounts sufficient to raise circulating hormone levels 10-fold 4 h after administration. thyroiditis was induced by immunization with rat thyroglobulin (tg) in complete freund's adjuvant, and the severity of the disease was assessed by comparison with saline-treated controls. thymic and splenic hypertrophy were found in t4-treated animals, whereas ... | 1985 | 3871692 |
| glucocorticoids enhance the in vitro ig synthesis of pokeweed mitogen-stimulated human b cells by inhibiting the suppressive effect of t8+ t cells. | the in vitro effects of three potent glucocorticoids (gc) (dexamethasone, prednisolone, cortisone) on human lymphocyte functions were investigated. in pharmacological concentrations gc strongly suppressed lymphocyte transformation induced by t-cell mitogen (concanavalin a and phytohaemagglutinin). after pokeweed mitogen (pwm) stimulation gc enhanced the number of immunoglobulin (ig)-producing cells without affecting the proliferative response. mineralocorticoid aldosterone showed no effect. addi ... | 1985 | 3871537 |
| ontogeny of the t-cell antigen receptor within the thymus. | the expression of t-cell antigen receptors during t-cell ontogeny is an important issue that bears directly on such questions as where t-cell tolerance is acquired, at what stage t cells become susceptible to repertoire selection, and why most thymocytes die within the thymus. the thymus rudiment is colonized during days 11 and 12 of gestation, but it is not until day 19 that significant numbers of functional thymocytes are present. although much is known about the ontogeny of function- and spec ... | 1985 | 3871511 |
| purification and properties of the bacteriophage t4 gene 61 rna priming protein. | the bacteriophage t4 gene 61 protein is required, together with the gene 41 protein and single-stranded dna, for the synthesis of the pentaribonucleotides that are used as primers for the start of each new okazaki dna fragment during t4 dna replication. using this priming activity as an assay, we have purified the 61 protein to essential homogeneity in milligram amounts. the priming activity was identified with the product of t4 gene 61 by using two-dimensional polyacrylamide gel electrophoresis ... | 1985 | 3871437 |
| pregnancy-associated growth factor. i. a proliferative agent which expands adult and cord t4 cells in unfractionated cultures. | experiments were designed to examine the effects of pregnancy-associated growth factor (pagf), a substance found in commercial preparations of crude human chorionic gonadotropin (hcg), on unfractionated human cord blood cells (cbc) and adult peripheral blood lymphocytes (pbl) cultured in 5% fetal calf serum (fcs). comparisons of pagf-induced [3h]tdr incorporation in nine pairs of simultaneously cultured cbc and pbl with phytohemagglutinin (pha) and tetanus toxoid (tt) showed that all cbc and pbl ... | 1985 | 3871378 |
| modification of the contractile activity of isolated rat atria by lectin-activated t-lymphocyte subsets. | we had previously shown that human t-lymphocytes (erfc) that had been activated for a short time with phytohemagglutinin (pha) produced positive inotropic and chronotropic effects on spontaneously beating rat atria (sterin-borda, l., et al., naunyn scmiedeberg's arch. pharmacol. 324, 58, 1983). in this study, we first prepared t4-rich (t4) and t8-rich (t8) cells from erfc by selective lysis with okt4 and okt8 monoclonal antibodies and rabbit complement. then, we tested the effect of pha-stimulat ... | 1985 | 3871361 |
| the isolation and sequence of the gene encoding t8: a molecule defining functional classes of t lymphocytes. | the t cell surface glycoproteins t4 and t8 are thought to mediate efficient cell-cell interactions in the immune system and in this way may be responsible for the appropriate targeting of subpopulations of t cells. we have used gene transfer combined with subtractive hybridization to isolate both cdna and functional genomic clones encoding the t8 protein. the sequence of the cdna reveals that t8 is a transmembrane protein with an n-terminal domain which shares significant homology to immunoglobu ... | 1985 | 3871356 |
| atopic asthma: t-cell response to corticosteroids. | atopic asthma is associated with diminished cell-mediated immunity and elevated levels of ige, both of which may be caused by imbalances of t-lymphocyte subsets. we analyzed the response of peripheral blood t-cell subsets to two commonly used corticosteroid preparations as a probe of t-cell subset regulation. we administered prednisone (p) 60 mg or 20 mg, beclomethasone dipropionate (bdp) aerosol, 336 micrograms, placebo, or bdp vehicle in a double-blind protocol to 15 atopic asthmatic patients ... | 1985 | 3871188 |
| t cell chronic lymphocytic leukemia with lymphocytes of unusual immunologic phenotype and function. | this report discusses a case of t cell chronic lymphocytic leukemia (t-cll) in an elderly white man whose lymphocytes expressed a post-thymic phenotype except for the coexpression of t4 and t8 on 80% to 95% of the cells. because of the uncommon phenotype, in vitro functional assays were performed that showed decreased mitogenic responses but normal helper activity for b cell immunoglobulin secretion and normal suppressor activity of lectin-induced mitogenesis. morphologic evaluation by both ligh ... | 1985 | 3871160 |
| plain-film criteria for excluding aortic rupture in blunt chest trauma. | emergency room supine chest films of 86 consecutive patients with blunt chest trauma and possible aortic rupture were reviewed. sixteen radiographic signs were analyzed independently and in combination. only two signs associated with aortic rupture were statistically significant: deviation of the nasogastric tube to the right at the t4 level and depression of the left main-stem bronchus below 40 degrees from the horizontal. false positives and false negatives occurred with each individual radiog ... | 1985 | 3871138 |
| identification and characterization of a b cell growth inhibitory factor (bif) on bcgf-dependent b cell proliferation. | the culture supernatants of con a-activated human peripheral blood mononuclear cells (pbm) contained at least two regulatory factors upon b cell proliferation. one was b cell growth factor (bcgf), which activated antigen-stimulated b cells to proliferation and clonal expansion, and the other was its inhibitory factor, arbitrarily named b cell growth inhibitory factor (bif). this bif inhibited the effect of bcgf on anti-mu-stimulated b cells or the monoclonal mature b cell line (cll-t.h.) obtaine ... | 1985 | 3871108 |
| murine anti-swine t4 and t8 monoclonal antibodies: distribution and effects on proliferative and cytotoxic t cells. | two murine monoclonal antibodies reactive with porcine nylon wool-nonadherent t cells were examined by flow microfluorometry analysis for cellular distribution, and by functional analysis for their effect on mlr and ctl assays. the two determinants recognized, called pt4 and pt8, were shown to be expressed on almost mutually exclusive cell populations, with only 6 to 15% of t cells expressing both antigens. among the 15% of nylon wool-nonadherent cells that did not express either pt4 or pt8, 5% ... | 1985 | 3871107 |
| plasma concentrations of thyroid hormones in dogs: influence of sampling hour, breed and age. | thyroxine (t4) and triiodothyronine (t3) plasma concentrations have been determined during 24-hr sampling periods in six mongrels (age 12-36 months), six beagles (age 35-37 months), three labradors (age 3.5 months) and three beagles (age 5 months). the mean t4 levels of the labradors were significantly lower than the values found for mongrels or older beagles (p less than 0.05), whereas t3 was higher in the 5 month old beagles compared to the mongrels (p less than 0.001), young beagles (p less t ... | 1985 | 3870842 |
| a study on antisperm antibody in homosexual men. | using a hemagglutination assay we surveyed antibody distribution to human sperm antigen in a sexually transmitted disease (std) clinic population and in an aids study group. we found no significant difference for the mean antisperm antibody titres between sexually active heterosexual men, women or homosexual men, homosexual men with or without aids, and no correlation between t4/t8 lymphocyte ratios and antibody titres in homosexual men. we conclude that levels of antibody to sperm antigens are ... | 1985 | 3868684 |
| lack of maternal metabolic, endocrine, and environmental influences in the etiology of cleft lip with or without cleft palate. | we studied metabolic, endocrine, and environmental factors in 59 women who had delivered a child with cleft lip with or without cleft palate (cl +/- cp) and compared these values with those of 56 mothers of unaffected children. there was no significant difference between the two groups with respect to race, age, weight, height, education, parity, menstrual history, medical illnesses, or the use of contraceptives, tobacco, alcohol, or caffeine. all patients had a normal xx karyotype confirmed by ... | 1985 | 3868431 |
| alterations in vitamin a and thyroid hormone status in anorexia nervosa and associated disorders. | vitamin a and thyroid hormone status was investigated in 27 patients with anorexia nervosa. subjects were divided into three groups based on eating behavior and serum carotene concentrations: anorexic (dietary restriction), normal carotene; anorexic, elevated serum carotene; bulimic, elevated serum carotene. all bulimic subjects fulfilling selection criteria were hypercarotenemic (weight loss and reduced metabolic rate). data were compared to normal healthy volunteers. serum retinol and retinol- ... | 1985 | 3865529 |
| ion etching bacteriophage t4: support for a spiral-fold model of packaged dna. | ion etching of bacteriophage t4 erodes virus components progressively from the outside to the inside while preserving the overall structure. the terminal portion of the t4 dna molecule packaged can be specifically radiolabeled and was found to be eroded more rapidly than the remainder of the dna. this strongly suggests that the first dna to enter the prohead is condensed in the center of the capsid and is therefore shielded from the ion beam by the surrounding last packaged dna. the results supp ... | 1985 | 3865208 |
| genetic complementation of uv-induced dna repair in chinese hamster ovary cells by the denv gene of phage t4. | the denv gene of phage t4, encoding the pyrimidine dimer-specific dna repair enzyme endonuclease v, has been introduced by dna transfection into the uv-sensitive dna repair-deficient chinese hamster ovary (cho) cell line uv5. transformants were first selected for resistance to the antibiotic g418 conferred by the neo gene from tn5 carried by the same plasmid. a majority of the isolated g418-resistant uv5 clones also showed an increased resistance to 254-nm uv light. one clone, designated i-a1, w ... | 1985 | 3865186 |
| multiple class i and class ii major histocompatibility complex allospecificities are generated with t cell receptor variable (v) domains created by a single ti beta v gene family. | 10 alloreactive cytotoxic t lymphocytes using rex ti beta variable region (v) gene segments in formation of their antigen/major histocompatibility complex (mhc) t3-ti receptor were selected, cloned, and characterized in an effort to examine the extent of receptor diversity created by this one v gene family. multiple and distinct class ii as well as class i allospecificities were generated from the formation of different ti beta v domains. five allospecificities were directed at various class i e ... | 1985 | 3862747 |
| perturbation of maintenance and de novo dna methylation in vitro by uvb (280-340 nm)-induced pyrimidine photodimers. | the effect of pyrimidine photodimers on transmethylation reactions catalyzed by a highly purified rat liver dna (cytosine-5-)-methyltransferase (ec 2.1.1.37) that exhibits maintenance and de novo methylation activities was studied in vitro, using the viral substrates m13 mp9 replicative form (rf) dna and the hemimethylated analog formed from primed synthesis of phage dna in the presence of 2'-deoxy-5-methylcytidine 5'-triphosphate. these dnas were irradiated with uvb (280-340 nm) at 900-3600 j/m ... | 1985 | 3862117 |
| interaction between intestinal and peripheral blood mononuclear cells. | effective methods are available to isolated immunocytes from human intestinal mucosa. isolated mononuclear cells from the mucosa of patients with crohn's disease and normal controls were classified according to ultrastructure and cell-surface profile by the use of a panel of monoclonal antibodies. in both compartments of the mucosa, i.e., the lamina propria and the epithelial layer, no differences were found between intestinal tissue from control patients and unaffected mucosa of patients with c ... | 1985 | 3861037 |
| [combination effects of cddp, acr and hcfu on progressive urothelial tumors]. | nine patients with progressive urothelial tumors treated with combination chemotherapy were evaluated. the median age of the patients was fifty-four; 6 men and 3 women; 7 of the tumors were of the renal pelvis and/or ureter and at stage c or d at the time of initial chemotherapy. another of the tumors was triple cancer (bladder, prostate, sigmoid) and the remaining case was of bladder cancer. the stage of the two bladders cancers was t4. all patients were treated with cddp (cis-platinum), acr (a ... | 1985 | 3859251 |
| immunologic alterations in monkeys with simian acquired immunodeficiency syndrome (saids). | levels of lymphocyte responsiveness to t- and b-cell-specific mitogens and expressions of ia, t4, t8, and t11 surface markers were monitored during the course of simian acquired immunodeficiency syndrome (saids) in four rhesus macaques that either died or became ill and survived. the monkey that died showed progressively suppressed responses to concanavalin a (con a), phytohemagglutinin (pha), pokeweed mitogen (pwm), and staphylococcus aureus cowan i strain (sac) through the time of death (5 1/2 ... | 1985 | 3857635 |
| a genetic screen for mutations that increase the thermal stability of phage t4 lysozyme. | a method has been developed to screen for mutants of phage t4 lysozyme that are more stable than the wild-type enzyme. using an assay that detects lysozyme activity on petri plates [streisinger, g., okada, y., emrich, j., newton, j., tsugita, a., terzaghi, e. & inouye, m. (1966) cold spring harbor symp. quant. biol. 31, 77-84], protein synthesized during the formation of phage plaques at a permissive temperature (33 degrees c) was tested for its ability to withstand incubation at a temperature t ... | 1985 | 3856227 |