Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
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| microwave-assisted synthesis of antimicrobial agents based on pyridazine moiety. | an efficient and simple microwave assisted synthesis of sulfonamide derivatives incorporating the pyridazine moiety has been developed. these sulfonamides were used for the preparation of new heterocyclic compounds via reaction with different reagents using a microwave irradiation technique. the structures of the newly synthesized compounds were confirmed on the basis of ftir, (1)h and (13)c-nmr, mass spectral techniques and elemental analyses. some of the new synthesized compounds were assayed ... | 2013 | 23163720 |
| impaired bactericidal but not fungicidal activity of polymorphonuclear neutrophils in patients with chronic lymphocytic leukemia. | we examined the qualitative polymorphonuclear neutrophil (pmn)-associated immune impairment in patients with chronic lymphocytic leukemia (cll) by characterizing phagocytic killing of key non-opsonized bacterial (staphylococcus aureus and pseudomonas aeruginosa) and fungal (candida albicans and aspergillus fumigatus) pathogens. neutrophils were collected from 47 non-neutropenic patients with cll (pmn count > 1000/mm(3)) and age-matched and young healthy controls (five each). a subset of patients ... | 2013 | 23163595 |
| current understanding of hog-mapk pathway in aspergillus fumigatus. | aspergillus fumigatus is an important opportunistic fungal pathogen that causes lethal systemic invasive aspergillosis. it must be able to adapt to stress in the microenvironment during host invasion and systemic spread. the high-osmolarity glycerol (hog) mitogen-activated protein kinase (mapk) signaling pathway is a key element that controls adaptation to environmental stress. it plays a critical role in the virulence of several fungal pathogens. in this review, we summarize the current knowled ... | 2013 | 23161019 |
| broad-spectrum antifungal-producing lactic acid bacteria and their application in fruit models. | a large-scale screen of some 7,000 presumptive lactic acid bacteria (lab), isolated from animal, human, or plant origin, identified 1,149 isolates with inhibitory activity against the food-spoilage mould penicillium expansum. in excess of 500 lab isolates were subsequently identified to produce a broad spectrum of activity against p. expansum, penicillium digitatum, penicillium notatum, penicillium roqueforti, rhizopus stolonifer, fusarium culmorum, aspergillus fumigatus and rhodotorula mucilagi ... | 2013 | 23160868 |
| nontuberculous mycobacteria, fungi, and opportunistic pathogens in unchlorinated drinking water in the netherlands. | the multiplication of opportunistic pathogens in drinking water supplies might pose a threat to public health. in this study, distributed unchlorinated drinking water from eight treatment plants in the netherlands was sampled and analyzed for fungi, nontuberculous mycobacteria (ntm), and several opportunistic pathogens by using selective quantitative pcr methods. fungi and ntm were detected in all drinking water samples, whereas legionella pneumophila, pseudomonas aeruginosa, stenotrophomonas ma ... | 2013 | 23160134 |
| radioiodinated peptide probe for selective detection of oxidized low density lipoprotein in atherosclerotic plaques. | despite the significant effort in developing radioprobes for atherosclerosis, few have low molecular weight. oxidized ldl (oxldl), a highly proinflammatory and proatherogenic factor that is abundant in atherosclerotic plaques, plays a pivotal role in plaque destabilization, which makes oxldl a relevant probe target. we developed a radioiodinated short peptide, ahp7, as a low molecular weight probe for specific oxldl imaging and evaluated its utility using myocardial infarction-prone watanabe her ... | 2013 | 23157986 |
| the interplay between vacuolar and siderophore-mediated iron storage in aspergillus fumigatus. | iron is an essential element for all eukaryotes but its excess has deleterious effects. aspergillus fumigatus produces extracellular siderophores for iron uptake and the intracellular siderophore ferricrocin (fc) for distribution and storage of iron. iron excess has previously been shown to increase the content of ferric fc and the expression of the putative vacuolar iron importer ccca (afua_4g12530), indicating a role of both the vacuole and fc in iron detoxification. in this study, we show tha ... | 2012 | 23151814 |
| biocidal activity of metalloacid-coated surfaces against multidrug-resistant microorganisms. | 2012 | 23148568 | |
| posaconazole pharmacodynamic target determination against wild-type and cyp51 mutant isolates of aspergillus fumigatus in an in vivo model of invasive pulmonary aspergillosis. | invasive pulmonary aspergillosis (ipa) is a devastating disease of immunocompromised patients. pharmacodynamic (pd) examination of antifungal drug therapy in ipa is one strategy that may improve outcomes. the current study explored the pd target of posaconazole in an immunocompromised murine model of ipa against 10 a. fumigatus isolates, including 4 cyp51 wild-type isolates and 6 isolates carrying cyp51 mutations conferring azole resistance. the posaconazole mic range was 0.25 to 8 mg/liter. fol ... | 2013 | 23147740 |
| the il-36 receptor pathway regulates aspergillus fumigatus-induced th1 and th17 responses. | il-1 drives th responses, particularly th17, in host defense. sharing the same co-receptor, the il-1 family member il-36 exhibits properties similar to those of il-1. in the present study, we investigated the role of il-36 in aspergillus fumigatus-induced human th responses. we observed that different morphological forms of a. fumigatus variably increase steady-state mrna of il-36 subfamily members. il-36α is not significantly induced by any morphological form of aspergillus. most strikingly, il ... | 2013 | 23147407 |
| ketoprofen impairs immunosuppression induced by severe sepsis and reveals an important role for prostaglandin e2. | the mechanism of immunosuppression induced by severe sepsis is not fully understood. the production of prostaglandin e2 (pge2) during sepsis is well known, but its role in long-term consequences of sepsis has not been explored. the current study evaluates the role of pge2 in the development of immunosuppression secondary to sepsis and its potential as therapeutic target. cecal ligation and puncture was used as an experimental model for sepsis induction in balb/c and c57bl/6 mice. immunosuppressi ... | 2012 | 23143054 |
| detection of aspergillus fumigatus pulmonary fungal infections in mice with (99m)tc-labeled morf oligomers targeting ribosomal rna. | invasive aspergillosis is a major cause of infectious morbidity and mortality in immunocompromised patients. the fungus aspergillus fumigatus (a. fumigatus) is the primary causative agent of invasive aspergillosis. however, a. fumigatus infections remain difficult to diagnose particularly in the early stages due to the lack of a rapid, sensitive and specific diagnostic approach. in this study, we investigated (99m)tc labeled morf oligomers targeting fungal ribosomal rna (rrna) for the imaging de ... | 2013 | 23142409 |
| the effect of lipoprotein-associated phospholipase a2 deficiency on pulmonary allergic responses in aspergillus fumigatus sensitized mice. | lipoprotein-associated phospholipase a2 (lp-pla2)/platelet-activating factor acetylhydrolase (paf-ah) has been implicated in the pathogenesis of cardiovascular disease. a therapeutic targeting of this enzyme was challenged by the concern that increased circulating platelet activating factor (paf) may predispose to or increase the severity of the allergic airway response. the aim of this study was to investigate whether lp-pla2 gene deficiency increases the risk of paf and ige-mediated inflammato ... | 2012 | 23140447 |
| biosynthesis of the fungal cell wall polysaccharide galactomannan requires intraluminal gdp-mannose. | fungal cell walls frequently contain a polymer of mannose and galactose called galactomannan. in the pathogenic filamentous fungus aspergillus fumigatus, this polysaccharide is made of a linear mannan backbone with side chains of galactofuran and is anchored to the plasma membrane via a glycosylphosphatidylinositol or is covalently linked to the cell wall. to date, the biosynthesis and significance of this polysaccharide are unknown. the present data demonstrate that deletion of the golgi udp-ga ... | 2012 | 23139423 |
| some southern african plant species used to treat helminth infections in ethnoveterinary medicine have excellent antifungal activities. | diseases caused by microorganisms and parasites remain a major challenge globally and particularly in sub-saharan africa to man and livestock. resistance to available antimicrobials and the high cost or unavailability of antimicrobials complicates matters. many rural people use plants to treat these infections. because some anthelmintics e.g. benzimidazoles also have good antifungal activity we examined the antifungal activity of extracts of 13 plant species used in southern africa to treat gast ... | 2012 | 23134805 |
| fungal infections associated with contaminated methylprednisolone in tennessee. | we investigated an outbreak of fungal infections of the central nervous system that occurred among patients who received epidural or paraspinal glucocorticoid injections of preservative-free methylprednisolone acetate prepared by a single compounding pharmacy. | 2012 | 23131029 |
| efficacy and pharmacodynamics of voriconazole combined with anidulafungin in azole-resistant invasive aspergillosis. | azole resistance is an emerging problem in the treatment of aspergillus fumigatus infections. combination therapy may be an alternative approach to improve therapeutic outcome in azole-resistant invasive aspergillosis (ia). the in vivo efficacy of voriconazole and anidulafungin was investigated in a non-neutropenic murine model of ia using voriconazole-susceptible and voriconazole-resistant a. fumigatus clinical isolates. | 2013 | 23129729 |
| differential proteomic analysis of aspergillus fumigatus morphotypes reveals putative drug targets. | aspergillus fumigatus is the main etiological agent of invasive aspergillosis, an important opportunistic infection for neutropenic patients. the main risk groups are patients with acute leukemia and bone marrow transplantation recipients. the lack of an early diagnostic test together with the limited spectrum of antifungal drugs remains a setback to the successful treatment of this disease. during invasive infection the inhaled fungal conidia enter the morphogenic cycle leading to angioinvasive ... | 2013 | 23128298 |
| stimulation of suicidal erythrocyte death by fumagillin. | fumagillin, a cyclohexane isolated from fungus aspergillus fumigatus, has anti-infective and anti-cancer potency. fumagillin is at least partially effective by inducing suicidal death or apoptosis. in analogy to apoptosis of nucleated cells, eryptosis is the suicidal death of erythrocytes characterized by cell shrinkage and cell membrane scrambling with phosphatidylserine exposure at the cell surface. stimulators of eryptosis include increase of cytosolic ca(2+)-activity ([ca(2+)](i)) and cerami ... | 2013 | 23121865 |
| incidence of fungal infections in chronic maxillary sinusitis. | fifty patients with chronic maxillary sinusitis were included in our study. antrat aspirate of chronically inflamed maxillary sinuses collected by antrat puncture were subjected to fungal culture. the study revealed the presence of fungi in 3 cases. aspergillus fumigatus was isolated in 2 cases while candida albicans was found in 1 case. the role of fungi, particularly aspergillus as pathogen is discussed in the context of antibiotics and immuno-suppressive therapy and local predisposing factors ... | 2000 | 23119648 |
| pharmacodynamics of anidulafungin against clinical aspergillus fumigatus isolates in a nonneutropenic murine model of disseminated aspergillosis. | azole resistance is an emerging increasing problem in aspergillus fumigatus that results in treatment failure. alternative treatments may improve the therapeutic outcome in patients with azole-resistant invasive aspergillosis (ia). little is known about the in vivo efficacy of the echinocandin anidulafungin (afg) in ia. the in vivo efficacy of 2.5, 5, 10, and 20 mg/kg of body weight afg was assessed against two clinical aspergillus fumigatus isolates with identical afg minimum effective concentr ... | 2013 | 23114773 |
| interaction of platelets and anidulafungin against aspergillus fumigatus. | the combination of platelets and anidulafungin at 0.03 μg/ml significantly (p < 0.05) reduced the germination rate and hyphal elongation in aspergillus fumigatus compared to those with either anidulafungin only or an untreated control. platelets decreased the expression of the fks gene, which plays an important role in cell wall synthesis. our results suggest that human platelets plus anidulafungin might contribute to defense against a. fumigatus. | 2013 | 23114752 |
| a controlled study to determine the efficacy of loxostylis alata (anacardiaceae) in the treatment of aspergillus in a chicken (gallus domesticus) model in comparison to ketoconazole. | the poultry industry due to intensive methods of farming is burdened with losses from numerous infectious agents, of which one is the fungus aspergillus fumigatus. in a preliminary study, the extracts of loxostylis alata a. spreng, ex rchb. showed good activity in vitro against a. fumigatus with a minimum inhibitory concentration of 0.07 mg/ml. for this study crude, a crude acetone extract of l. alata leaves was evaluated for its acute toxicity in a healthy chicken model and for efficacy in an i ... | 2012 | 23114185 |
| synthesis and antimicrobial activity of some novel cross-linked chitosan hydrogels. | four novel hydrogels based on chitosan were synthesized via a cross-linking reaction of chitosan with different concentrations of oxalyl bis 4-(2,5-dioxo-2h-pyrrol- 1(5h)-yl)benzamide. their structures were confirmed by fourier transform infrared x-ray (ftir), scanning electron microscopy (sem) and x-ray diffraction. the antimicrobial activities of the hydrogels against two crop-threatening pathogenic fungi namely: aspergillus fumigatus (a. fumigatus, rcmba 06002), and aspergillus niger (a. nige ... | 2012 | 23109847 |
| successful treatment of liver aspergilloma by caspofungin acetate first-line therapy in a non-immunocompromised patient. | aspergillosis remains to be a life-threatening complication in immunocompromised patients. however, aspergillus infection can be observed in non-immunocompromised individuals in rare cases. we report a case of liver aspergilloma in a chronic aplastic anemia patient under relatively intact immune status. therapeutic strategy for this rare condition was extensively discussed and caspofungin acetate single agent first-line therapy was applied after careful consideration. encouraging clinical and ra ... | 2012 | 23109838 |
| identification of the verruculogen prenyltransferase ftmpt3 by a combination of chemical, bioinformatic and biochemical approaches. | previous studies showed that verruculogen is the end product of a biosynthetic gene cluster for fumitremorgin-type alkaloids in aspergillus fumigatus and neosartorya fischeri. in this study, we isolated fumitremorgin a from n. fischeri. this led to the identification of the responsible gene, ftmpt3, for o-prenylation of an aliphatic hydroxy group in verruculogen. this gene was found at a different location in the genome of n. fischeri than the identified cluster. the coding sequence of ftmpt3 wa ... | 2012 | 23109474 |
| triazole fungicides and the selection of resistance to medical triazoles in the opportunistic mould aspergillus fumigatus. | azole resistance is an emerging problem in the opportunistic mould aspergillus fumigatus. the triazoles are the most important agents for the management of aspergillus diseases in humans. selection for acquired resistance may occur in the hospital setting through exposure to high doses of azoles during azole therapy, but evidence is accumulating that a. fumigatus may become resistant to medical triazoles through environmental exposure to fungicides. the recovery of a. fumigatus isolates resistan ... | 2013 | 23109245 |
| toxicity, antimicrobial and anthelmintic activities of vernonia guineensis benth. (asteraceae) crude extracts. | this study examined the antibacterial, antifungal, and anthelmintic properties of extracts obtained from the plant vernonia guineensis, a plant commonly used in traditional cameroonian medicine. | 2012 | 23107821 |
| the fungal α-aminoadipate pathway for lysine biosynthesis requires two enzymes of the aconitase family for the isomerization of homocitrate to homoisocitrate. | fungi produce α-aminoadipate, a precursor for penicillin and lysine via the α-aminoadipate pathway. despite the biotechnological importance of this pathway, the essential isomerization of homocitrate via homoaconitate to homoisocitrate has hardly been studied. therefore, we analysed the role of homoaconitases and aconitases in this isomerization. although we confirmed an essential contribution of homoaconitases from saccharomyces cerevisiae and aspergillus fumigatus, these enzymes only catalysed ... | 2012 | 23106124 |
| prenylation of flavonoids by using a dimethylallyltryptophan synthase, 7-dmats, from aspergillus fumigatus. | production of typical plant metabolites by a fungal enzyme: fungal prenyltransferases of the dmats superfamily are mainly involved in the biosynthesis of prenylated indole alkaloids, but also catalyze the prenylation of tyrosine and naphthalene derivatives. in this study, nine prenylated flavonoids were produced by using the recombinant dimethylallyltryptophan synthase 7-dmats from aspergillus fumigatus. | 2011 | 23106077 |
| expressed sequence tags of aspergillus fumigatus: extension of catalogue and their evaluation as putative drug targets and/or diagnostic markers. | aspergillus fumigatus a fungal pathogen is implicated in a spectrum of allergic and invasive disorders in humans. validation of transcriptome of pathogen is essential for understanding its virulence mechanism and to identify new therapeutic targets/diagnostic markers. in order to rapidly identify genes of aspergillus fumigatus we adopted sequencing of cdna clones. our earlier effort has lead to identification of 68 expressed sequence tags of aspergillus fumigatus. present study describes 52 more ... | 2009 | 23105821 |
| immune response to n-terminal and c-terminal deletion mutants of aspergillus fumigatus major allergen asp f 3. | the ubiquitous fungus aspergillus fumigatus causes allergic rhinitis, asthma, sinusitis and allergic bronchopulmonary aspergillosis. a number of major allergens from a. fumigatus are purified, but their structure-function role in the pathogenesis of disease is not known. such information is essential for devising alternative therapy of fungal allergic diseases. in the present study, n-terminal and c-terminal deletion mutants ofasp f 3 were constructed and their immunopathological responses studi ... | 2006 | 23105608 |
| immunoproteomic analysis of secretory proteins ofaspergillus fumigatus with specific ige immunoreactivity. | allergenic/antigenic proteins are known to induce type i and type iii hypersensitivity reactions leading to allergic bronchopulmonary aspergillosis (abpa) in immunocompetent host. the common structural features or intrinsic properties of the allergens/antigens leading to allergenicity in a host are not well understood. in the current report, comparative analysis of proteins on two dimensional gel electrophoresis (2d-3) and specific ige immunoblots ofa. fumigatus secretory proteins (1(st), 2(nd) ... | 2006 | 23105607 |
| identification and characterization of polyubiquitin gene from cdna library of aspergillus fumigatus. | aspergillus fumigatus (afu) causes allergic and invasive forms of diseases in humans. in order to identify genes relevant for pathogenesis, a total of 235 cdna clones were randomly selected and sequenced from cdna library of afu. one of the partially sequenced cdna clones was homologous to polyubiquitin. sequencing of the complete cdna clone showed an open reading frame of 912 bases. comparison with genomic sequence of afu using blastn program, revealed that polyubiquitin gene comprises of 992 b ... | 2005 | 23105524 |
| biochemical characterization of an immunodominant allergen/antigen ofaspergillus fumigatus. | an 18kda protein was identified as a major immunodominant allergen/antigen secreted by a wild type isolate and various clinical isolates ofa. fumigatus. the protein was purified to homogeneity and the n-terminal amino acid was found to be alanine. the n-terminal 20 amino acid sequence of 18kda was found to be similar to restrictocin, a cytotoxin secreted byaspergillus restrictus. mass spectroscopic analysis of the purified allergen revealed a molecular size of 17.01 kda. immunoreactivity of the ... | 1999 | 23105213 |
| detection ofaspergillus fumigatus based on asp fl gene in clinical specimens. | oligonucleotide primers were synthesised based on the gene sequence of an 18 kda allergen/antigen ofa. fumigatus isolated from a pathogenic strain. polymerase chain reaction (pcr) was carried out using the forward and reverse primers and genomic dna ofa. fumigatus, a. flavus anda. niger as template. this resulted in a pcr product of 480 bp with onlya. fumigatus. the absence of pcr product ina. flavus anda. niger with the primers of asp fl facilitated use of these primers for detection ofa. fumig ... | 1999 | 23105210 |
| dsc orthologs are required for hypoxia adaptation, triazole drug responses, and fungal virulence in aspergillus fumigatus. | hypoxia is an environmental stress encountered by aspergillus fumigatus during invasive pulmonary aspergillosis (ipa). the ability of this mold to adapt to hypoxia is important for fungal virulence and genetically regulated in part by the sterol regulatory element binding protein (srebp) srba. srba is required for fungal growth in the murine lung and to ultimately cause lethal disease in murine models of ipa. here we identified and partially characterized four genes (dsca, dscb, dscc, and dscd, ... | 2012 | 23104569 |
| invasive aspergillosis: drug-dispensing systems as a source of filamentous fungal contamination in high-risk units? | high-risk units with air-control measures at grenoble university hospital are equipped with automated dispensing systems that are filled daily using drug trolleys routed from the pharmacy to the ward. the aim of this study was to evaluate the level of filamentous fungi (ff) contamination present in trolleys under usual conditions and after cleaning with aniosurf(®) (fungicidal disinfectant). ff were detected in all samples, and 83.3% of samples were contaminated with aspergillus fumigatus. clean ... | 2012 | 23102819 |
| diversity and exploration of bioactive marine actinomycetes in the bay of bengal of the puducherry coast of india. | the present study was designed to investigate the puducherry coast of the bay of bengal, india for the diversity of bioactive actinomycetes. a total of 50 actinomycete strains were isolated from the marine sediments and most of the strains were belongs to streptomyces. these strains were identified by means of morphological physiological, biochemical and cultural characteristics. the isolates were subjected to shake flask fermentation and the secondary metabolites were extracted with ethyl aceta ... | 2010 | 23100811 |
| isolation and characterisation of phosphate solubilising microorganisms from the cold desert habitat of salix alba linn. in trans himalayan region of himachal pradesh. | phosphate solubilising microorganisms (psm) (bacteria and fungi) associated with salix alba linn. from lahaul and spiti valleys of himachal pradesh were isolated on pikovskaya (pvk), modified pikovskaya (mpvk) and national botanical research institute agar (nbrip) media by spread plating. the viable colony count of p-solubilising bacteria (psb) and fungi (psf) was higher in rhizosphere than that of non-rhizosphere. the frequency of psm was highest on mpvk followed by nbrip and pvk agar. the maxi ... | 2008 | 23100719 |
| bronchial colonisation in patients with lung cancer: a prospective study. | bronchial colonisation is frequently reported in patients with lung cancer, and has a potential impact on therapeutic management and prognosis. we aimed to prospectively define the prevalence and nature of bronchial colonisation in patients at the time of diagnosing lung cancer. 210 consecutive patients with lung cancer underwent a flexible bronchoscopy for lung cancer. the type and frequency of bacterial, mycobacterial and fungal colonisation were analysed and correlated with the patients' and ... | 2013 | 23100491 |
| [frequency of pulmonary mycoses determined by analyzing lung secretion samples]. | the frequency of pulmonary mycoses has increased in the past few years specially in immunocompromised patients. | 2012 | 23096664 |
| [cancer, febrile neutropenia and pulmonary images: findings in bronchoalveolar lavage in children]. | lung infections are a serious complication in children with cancer. bronchoalveolar lavage (bal) has been demonstrated to be an effective procedure for achieving etiologic diagnosis. | 2012 | 23096474 |
| breaking the regioselectivity of indole prenyltransferases: identification of regular c3-prenylated hexahydropyrrolo[2,3-b]indoles as side products of the regular c2-prenyltransferase ftmpt1. | the prenyltransferase ftmpt1 from aspergillus fumigatus is involved in the biosynthesis of fumitremorgin-type alkaloids and catalysed the regular c2-prenylation of brevianamide f (cyclo-l-trp-l-pro). it has been shown that ftmpt1 also accepted a number of other tryptophan-containing cyclic dipeptides and prenylated them, in the presence of dimethylallyl diphosphate, at c-2 of the indole nucleus. detailed analysis of the incubation mixtures of ftmpt1 with these cyclic dipeptides revealed the pres ... | 2012 | 23090579 |
| in vivo molecular microimaging of pulmonary aspergillosis. | the early diagnosis of invasive pulmonary aspergillosis (ipa) is challenging. fibered confocal fluorescence microscopy (fcfm) is a new technique that allows in vivo imaging of the lung microstructure during bronchoscopy. in this study, we investigated the ability of fcfm to detect a fluorescent peptide-tracer bound to aspergillus fumigatus in experimental ipa in 13 immunosuppressed, non-neutropenic rats. subpleural ipa microabscesses were imaged through a transthoracic window using fcfm in vivo ... | 2013 | 23088299 |
| vea regulates conidiation, gliotoxin production, and protease activity in the opportunistic human pathogen aspergillus fumigatus. | invasive aspergillosis by aspergillus fumigatus is a leading cause of infection-related mortality in immunocompromised patients. in this study, we show that vea, a major conserved regulatory gene that is unique to fungi, is necessary for normal morphogenesis in this medically relevant fungus. although deletion of vea results in a strain with reduced conidiation, overexpression of this gene further reduced conidial production, indicating that vea has a major role as a regulator of development in ... | 2012 | 23087369 |
| successful resumption of peritoneal dialysis after aspergillus fumigatus peritonitis. | 2012 | 23084928 | |
| the function and evolution of the aspergillus genome. | species in the filamentous fungal genus aspergillus display a wide diversity of lifestyles and are of great importance to humans. the decoding of genome sequences from a dozen species that vary widely in their degree of evolutionary affinity has galvanized studies of the function and evolution of the aspergillus genome in clinical, industrial, and agricultural environments. here, we synthesize recent key findings that shed light on the architecture of the aspergillus genome, on the molecular fou ... | 2013 | 23084572 |
| the index case for the fungal meningitis outbreak in the united states. | persistent neutrophilic meningitis presents a diagnostic challenge, because the differential diagnosis is broad and includes atypical infectious causes. we describe a case of persistent neutrophilic meningitis due to aspergillus fumigatus in an immunocompetent man who had no evidence of sinopulmonary or cutaneous disease. an epidural glucocorticoid injection was identified as a potential route of entry for this organism into the central nervous system, and the case was reported to the state heal ... | 2012 | 23083311 |
| aspergillus nidulans galactofuranose biosynthesis affects antifungal drug sensitivity. | the cell wall is essential for fungal survival in natural environments. many fungal wall carbohydrates are absent from humans, so they are a promising source of antifungal drug targets. galactofuranose (galf) is a sugar that decorates certain carbohydrates and lipids. it comprises about 5% of the aspergillus fumigatus cell wall, and may play a role in systemic aspergillosis. we are studying aspergillus wall formation in the tractable model system, a. nidulans. previously we showed single-gene de ... | 2012 | 23078837 |
| the role of dendritic cells in the innate recognition of pathogenic fungi (a. fumigatus, c. neoformans and c. albicans). | dendritic cells (dcs) are the bridge between the innate and adaptive immune system. dcs are responsible for sensing and patrolling the environment, initiating a host response and instructing the proper adaptive immune response against pathogens. recent advances in medical treatments have led to increased use of immunosuppressive drugs, leading to the emergence of fungal species that cause life-threatening infections in humans. three of these opportunistic fungal pathogens: aspergillus fumigatus, ... | 2012 | 23076328 |
| multistate outbreak of fungal infection associated with injection of methylprednisolone acetate solution from a single compounding pharmacy - united states, 2012. | on september 18, 2012, the tennessee department of health was alerted by a clinician regarding a patient with culture-confirmed aspergillus fumigatus meningitis diagnosed 46 days after epidural steroid injection at a tennessee ambulatory surgical center. by september 27, the initial investigation, carried out by the tennessee department of health in collaboration with cdc and the north carolina department of health and human services, had identified an additional eight patients with clinically d ... | 2012 | 23076093 |
| bacterial inactivation by a singlet oxygen bubbler: identifying factors controlling the toxicity of (1)o2 bubbles. | a microphotoreactor device was developed to generate bubbles (1.4 mm diameter, 90 μl) containing singlet oxygen at levels toxic to bacteria and fungus. as singlet oxygen decays rapidly to triplet oxygen, the bubbles leave behind no waste or byproducts other than o(2). from a comparative study in deaerated, air saturated, and oxygenated solutions, it was reasoned that the singlet oxygen bubbles inactivate escherichia coli and aspergillus fumigatus, mainly by an oxygen gradient inside and outside ... | 2012 | 23075418 |
| effect of phosphatidylcholine on pld gene expression level of aspergillus fumigatus by the real time pcr method and investigations of these genes using bioinformatics analysis. | phospholipases are a group of enzymes that breakdown phospholipid molecules producing second products. these second products play a diverse role in the cell such as signal transduction and digestion in humans. in this study, the effect of phospholipids on the expression of pld genes of a. fumigatus was investigated. the pld genes of this fungus were also investigated using bioinformatics studies. | 2012 | 23066489 |
| interaction of the pathogenic mold aspergillus fumigatus with lung epithelial cells. | aspergillus fumigatus is an opportunistic environmental mold that can cause severe allergic responses in atopic individuals and poses a life-threatening risk for severely immunocompromised patients. infection is caused by inhalation of fungal spores (conidia) into the lungs. the initial point of contact between the fungus and the host is a monolayer of lung epithelial cells. understanding how these cells react to fungal contact is crucial to elucidating the pathobiology of aspergillus-related di ... | 2012 | 23055997 |
| expression and characterization of a novel enantioselective lipase from aspergillus fumigatus. | a 1,080-bp cdna (cgmcc 2873) encoding of a cold-active lipase of aspergillus fumigatus (afl67) was cloned and expressed in escherichia coli for the first time. the new lipase, afl67, was one-step purified by 8.30 folds through ni-nta affinity chromatography with a recovery of 86.8 %. the specific activity of purified afl67 was 449 u mg(-1) on p-np hexanoate. afl67 preferentially hydrolyzed p-nitrophenyl esters of short- and medium-chain fatty acids, with p-nitrophenyl hexanoate the maximum. the ... | 2012 | 23054817 |
| optimization of decolorization of palm oil mill effluent (pome) by growing cultures of aspergillus fumigatus using response surface methodology. | the conventional treatment process of palm oil mill effluent (pome) produces a highly colored effluent. colored compounds in pome cause reduction in photosynthetic activities, produce carcinogenic by-products in drinking water, chelate with metal ions, and are toxic to aquatic biota. thus, failure of conventional treatment methods to decolorize pome has become an important problem to be addressed as color has emerged as a critical water quality parameter for many countries such as malaysia. aspe ... | 2013 | 23054764 |
| aspergillus fumigatus and other thermophilic fungi in nests of wetland birds. | a study was performed on the numbers and species diversity of thermophilic fungi (growing at 45 °c in vitro) in 38 nests of 9 species of wetland birds, taking into account the physicochemical properties of the nests and the bird species. it was found that in nests with the maximum weight (nests of mute swan), the number and diversity of thermophilic fungi were significantly greater than in other nests, with lower weight. the diversity of the thermophilic biota was positively correlated with the ... | 2013 | 23054328 |
| synthesis, characterization, biological evaluation and qsar studies of 11-p-substituted phenyl-12-phenyl-11a,12-dihydro-11h-indeno[2,1-c][1,5]benzothiazepines as potential antimicrobial agents. | a novel series of 11-p-substituted phenyl-12-phenyl-11a,12-dihydro-11h-indeno[2,1-c][1,5]benzothiazepines (3) has been synthesized and screened for their antimicrobial activity against gram-positive (bacillus subtilis and staphylococcus aureus) and gram-negative (escherichia coli and pseudomonas aeruginosa) bacteria and fungi (aspergillus fumigates and candida albicans). the antimicrobial evaluation data indicated that the compounds, 3d, 3g, 3h, 3k-3p exhibited very promising antibacterial activ ... | 2012 | 23047232 |
| superoxide scavenging and antimicrobial activities of novel transition metal complexes of oxydiacetate dianion as primary ligand: spectral characterization, cyclic voltammetric investigations and crystal structure. | complexes of stoichiometries [m(oda)(4-pic)h(2)o]·xh(2)o [m = cu(1), x = 2; co (2), x = 4; ni (3), x = 2; ] [fe(oda)(4-pic)]·cl (4) and {[cu(2-pa)(2)]·2h(2)o}(n) (5) (h(2)oda = oxydiacetic acid, 4-pic = 4-picoline and 2-pah = 2-picolinic acid) were prepared to explore their uses as possible models for mono- and poly-nuclear metallo-pharmaceuticals. the chemical structures were elucidated by ir, fab-mass, (1)h, (13)c nmr, epr and uv-visible (ligand field) spectral analyses. electrochemical invest ... | 2012 | 23047227 |
| method for identifying heat-resistant fungi of the genus neosartorya. | species of the genus neosartorya are heat-resistant fungi that cause the spoilage of heat-processed acidic foods due to the formation of heat-resistant ascospores, and they produce mycotoxins, such as fumitremorgins and gliotoxin. their anamorphs are phylogenetically and morphologically very close to aspergillus fumigatus, which has never been reported as a spoilage agent in heat-processed food products. therefore it is important to discriminate between the species of neosartorya and a. fumigatu ... | 2012 | 23043829 |
| identification of the nad(p)h binding site of eukaryotic udp-galactopyranose mutase. | udp-galactopyranose mutase (ugm) plays an essential role in galactofuranose biosynthesis in microorganisms by catalyzing the conversion of udp-galactopyranose to udp-galactofuranose. the enzyme has gained attention recently as a promising target for the design of new antifungal, antitrypanosomal, and antileishmanial agents. here we report the first crystal structure of ugm complexed with its redox partner nad(p)h. kinetic protein crystallography was used to obtain structures of oxidized aspergil ... | 2012 | 23036087 |
| disseminated trichosporon mycotoxinivorans, aspergillus fumigatus, and scedosporium apiospermum coinfection after lung and liver transplantation in a cystic fibrosis patient. | trichosporon mycotoxinivorans is a novel pathogen recently found in cystic fibrosis patients. we report the first case of a disseminated fatal infection with t. mycotoxinivorans associated with invasive aspergillus fumigatus and scedosporium apiospermum infection after lung and liver transplantation in a cystic fibrosis patient. | 2012 | 23035187 |
| comparison of transcriptome technologies in the pathogenic fungus aspergillus fumigatus reveals novel insights into the genome and mpka dependent gene expression. | the filamentous fungus aspergillus fumigatus has become the most important airborne fungal pathogen causing life-threatening infections in immuno-compromised patients. recently developed high-throughput transcriptome and proteome technologies, such as microarrays, rna deep-sequencing, and lc-ms/ms of peptide mixtures, are of enormous value for systematically investigating pathogenic organisms. in the field of infection biology, one of the priorities is to collect and standardise data, in order t ... | 2012 | 23031507 |
| evaluation of serum galactomannan detection for diagnosis of feline upper respiratory tract aspergillosis. | measurement of serum galactomannan (gm), a polysaccharide fungal cell-wall component, is a non-invasive test for early diagnosis of invasive aspergillosis in humans. feline upper respiratory tract (urt) aspergillosis is an emerging infectious disease in cats. diagnosis requires biopsy for procurement of tissue specimens for cytological or histological detection of fungal hyphae and for fungal culture. the aim of this study was to evaluate serum gm measurement as a non-invasive diagnostic test fo ... | 2013 | 23031342 |
| aspergillus-pcr in bronchoalveolar lavage for detection of invasive pulmonary aspergillosis in immunocompromised patients. | invasive fungal disease (ifd) is a frequent and serious infectious complication in immunocompromised patients. culture and cytology in bronchoalveolar lavage (bal) have a high specificity but low sensitivity for the diagnosis of ifd as assessed by histology. molecular methods are expected to allow a rapid diagnosis of ifd with a high sensitivity. we evaluated the diagnostic accuracy of conventional nested pcr in the bronchoalveolar fluid to diagnose ifd in severely immunocompromised patients. | 2012 | 23031334 |
| differential strain-related tissue immune response to sublethal systemic aspergillus fumigatus infection in mice. | using a nonlethal systemic aspergillus fumigatus infection, we have recently shown that similarly efficient elimination of fungus from spleens of prototypic th1 (c57bl/6) and prototypic th2 (balb/c) mice is associated with differential immune responses. in light of these data and given the disseminated character of infection, the aim of the present study is to explore whether there are also strain-dependent differences in antifungal responses in peripheral tissues of infected mice. although simi ... | 2013 | 23030850 |
| the aspergillus fumigatus dihydroxyacid dehydratase ilv3a/ilvc is required for full virulence. | dihydroxyacid dehydratase (dhad) is a key enzyme in the branched-chain amino acid biosynthetic pathway that exists in a variety of organisms, including fungi, plants and bacteria, but not humans. in this study we identified four putative dhad genes from the filamentous fungus aspergillus fumigatus by homology to saccharomyces cerevisiae ilv3. two of these genes, afua_2g14210 and afua_1g03550, initially designated afilv3a and afilv3b for this study, clustered in the same group as s. cerevisiae il ... | 2012 | 23028460 |
| biosorption of mercury (ii) from aqueous solutions onto fungal biomass. | the biosorption of mercury (ii) on 14 fungal biomasses, aspergillus flavus i-v, aspergillus fumigatus i-ii, helminthosporium sp., cladosporium sp., mucor rouxii mutant, m. rouxii im-80, mucor sp 1 and 2, and candida albicans, was studied in this work. it was found that the biomasses of the fungus m. rouxii im-80, m. rouxii mutant, mucor sp1, and mucor sp 2 were very efficient removing the metal in solution, using dithizone, reaching the next percentage of removals: 95.3%, 88.7%, 80.4%, and 78.3% ... | 2012 | 23028382 |
| detection of biofilm-grown aspergillus fumigatus by means of atomic force spectroscopy: ultrastructural effects of alginate lyase. | aspergillus fumigatus has become a leading cause of fungal morbidity and mortality, especially in immunocompromised patients. this fungus is able to grow as a multicellular community and produce a hydrophobic extracellular matrix (ecm), mainly composed of galactomannan and α-1,3 glucans, to protect itself from host defenses and antimicrobial drugs. this matrix envelops the fungus hyphae, binding them into a contiguous sheath on the colony surface, forming a biofilm and increasing the fungal resi ... | 2012 | 23026204 |
| protective effector cells of the recombinant asp f3 anti-aspergillosis vaccine. | an aspergillus fumigatus vaccine based on recombinant asp f3-protein has the potential to prevent aspergillosis in humans, a devastating fungal disease that is the prime obstacle to the success of hematopoietic cell transplantation. this vaccine protects cortisone acetate (ca)-immunosuppressed mice from invasive pulmonary aspergillosis via cd4(+) t cell mediators. aside from these mediators, the nature of downstream fungicidal effectors is not well understood. neutrophils and macrophages protect ... | 2012 | 23024640 |
| nosa, a transcription factor important in aspergillus fumigatus stress and developmental response, rescues the germination defect of a laea deletion. | aspergillus fumigatus is an increasingly serious pathogen of immunocompromised patients, causing the often fatal disease invasive aspergillosis (ia). one a. fumigatus virulence determinant of ia is laea, a conserved virulence factor in pathogenic fungi. to further understand the role of laea in ia, the expression profile of δlaea was compared to wild type, and several transcription factors were found significantly misregulated by laea loss. one of the transcription factors up-regulated over 4-fo ... | 2012 | 23022264 |
| unraveling the antifungal activity of a south american rattlesnake toxin crotamine. | crotamine is a highly basic peptide from the venom of crotalus durissus terrificus rattlesnake. its common gene ancestry and structural similarity with the β-defensins, mainly due to an identical disulfide bond pattern, stimulated us to assess the antimicrobial properties of native, recombinant, and chemically synthesized crotamine. antimicrobial activities against standard strains and clinical isolates were analyzed by the colorimetric microdilution method showing a weak antibacterial activity ... | 2013 | 23022146 |
| occurrence of household mold and efficacy of sodium hypochlorite disinfectant. | the occurrence and distribution of mold on household surfaces and the efficacy of bleach-based (sodium hypochlorite, naocl) disinfectants on mold viability and allergenicity was documented. household microenvironments prone to increased moisture were specifically targeted. using the sticky tape method, 1330 samples were collected from non-porous indoor surfaces of 160 homes across the united states, and analyzed for mold. homes were randomly selected and recruited via phone interviews. culture a ... | 2012 | 23016564 |
| reaginic antibodies from horses with recurrent airway obstruction produce mast cell stimulation. | reaginic antibodies (ige and some igg subclasses) and mast cells play important roles in the induction of type i immediate hypersensitivity reactions. these antibodies bind through their fc fragment to high affinity receptors (fcεri) present in the membrane of mast cells and basophils. the cross-linking of the receptor initiates a coordinated sequence of biochemical and morphological events that results in exocytosis of secretory granules containing pre-formed inflammatory mediators, secretion o ... | 2012 | 23011757 |
| loss of caveolin-1 from bronchial epithelial cells and monocytes in human subjects with asthma. | caveolin-1 has emerged as a critical regulator of signaling pathways involved in lung fibrosis and inflammation. | 2012 | 23004679 |
| the putative guanine nucleotide exchange factor rica mediates upstream signaling for growth and development in aspergillus. | heterotrimeric g proteins (g proteins) govern growth, development, and secondary metabolism in various fungi. here, we characterized rica, which encodes a putative gdp/gtp exchange factor for g proteins in the model fungus aspergillus nidulans and the opportunistic human pathogen aspergillus fumigatus. in both species, rica mrna accumulates during vegetative growth and early developmental phases, but it is not present in spores. the deletion of rica results in severely impaired colony growth and ... | 2012 | 23002107 |
| efficacy of liposomal amphotericin b for prophylaxis of acute or reactivation models of invasive pulmonary aspergillosis. | the efficacy of antifungal prophylaxis for prevention of invasive aspergillosis (ia) may depend on whether ia results from recent inhalation of spores or reactivation of latent colonisation. compare the efficacy of liposomal amphotericin b (lamb) for prophylaxis in acute and reactivation models of ia. in the acute model, mice immunosuppressed from day 0 were challenged at day 3 with an aerosol of aspergillus fumigatus. lamb (15 mg kg(-1) ) was administered at day 0 or at challenge. in the reacti ... | 2013 | 22998025 |
| development and application of mold antigen-specific enzyme-linked immunosorbent assays (elisa) to quantify airborne antigen exposure. | the aim of our study was to develop specific enzyme-linked immunosorbent assays (elisas) and apply these to assess mold antigen exposure in composting plants. sandwich elisas based on polyclonal antibodies to aspergillus fumigatus (af), penicillium chrysogenum (pc), and cladosporium herbarum (ch) antigens were developed and validated. reactivity to 18 different mold species was tested. to optimize extraction procedure, inhalable dust samples taken by a parallel sampler were extracted with or wit ... | 2012 | 22994572 |
| fatal outcome after heart transplantation caused by aspergillus lentulus. | opportunistic invasive fungal infections are a major cause of mortality in immunocompromised patients. early diagnosis of invasive aspergillosis and proper identification of the causative agent is crucial for guidance of therapy. accurate differentiation of aspergillus lentulus, a filamentous fungus often misidentified as atypical aspergillus fumigatus, is of concern as multiple antifungal drugs show a reduced susceptibility. this is the first report, to our knowledge, of a proven pulmonary inva ... | 2012 | 22988985 |
| breakthrough pulmonary aspergillus fumigatus infection with multiple triazole resistance in a spanish patient with chronic myeloid leukemia. | an allogeneic hematopoietic cell transplantation (allo-hct) patient presented with chronic pulmonary aspergillosis associated to pulmonary graft versus host disease (gvhd) and was treated for a long time with several antifungal agents that were administered as prophylaxis, combination therapies, and maintenance treatment. the patient suffered from a breakthrough invasive pulmonary aspergillosis due to aspergillus fumigatus after long-term antifungal therapy. | 2013 | 22986228 |
| validation and application of hplc-esi-ms/ms method for the quantification of rbbr decolorization, a model for highly toxic molecules, using several fungi strains. | a novel analytical method using hplc-ms/ms operating in selected reaction monitoring (srm) for evaluation of fungi efficacy to decolorize remazol brilliant blue r (rbbr) dye solution was developed, validated and applied. the method shows high sensibility allowing the detection of 4.6 pm of rbbr. four fungal strains were tested in liquid medium, three strains of aspergillus (aspergillus aculeatus, aspergillus flavus and aspergillus fumigatus) and phanerochaete chrysosporium. all fungi were able t ... | 2012 | 22985849 |
| effect of allergy and inflammation on eicosanoid gene expression in cftr deficiency. | allergic bronchopulmonary aspergillosis (abpa) is a complicating factor in cystic fibrosis (cf), affecting 2-15% of patients. we hypothesized that sensitization/challenge of cftr(-/-) mice with an aspergillus fumigatus (af) extract will affect eicosanoid pathway gene expression, impacting abpa and cf. | 2013 | 22985691 |
| ultraviolet irradiation: an effective inactivation method of aspergillus spp. in water for the control of waterborne nosocomial aspergillosis. | invasive aspergillosis is the second most common cause of nosocomial fungal infections and occurring mainly by aspergillus fumigatus, aspergillus flavus, and aspergillus niger. there is evidence that nosocomial aspergillosis may be waterborne. this study was conducted to evaluate the ultraviolet (uv) irradiation efficiency in terms of inactivating the most important aspergillus species in water since these are potential sources for nosocomial aspergillosis. a continuous flow uv reactor which cou ... | 2012 | 22985523 |
| highlights on molecular identification of closely related species. | the term "complex" emerged in the literature at the beginning of the genomic era associated to taxonomy and grouping organisms that belong to different species but exhibited similar patterns according to their morphological, physiological and/or other phenotypic features. dna-dna hybridization values ~70% and high identity on 16s rrna gene sequences were recommended for species delineation. electrophoretic methods showed in some cases to be useful for species identification and population struct ... | 2013 | 22982158 |
| microsatellite typing of aspergillus flavus from clinical and environmental avian isolates. | aspergillosis is one of the most common causes of death in captive birds. aspergillus fumigatus accounts for approximately 95 % of aspergillosis cases and aspergillus flavus is the second most frequent organism associated with avian infections. in the present study, the fungi were grown from avian clinical samples (post-mortem lung material) and environmental samples (eggs, food and litter). microsatellite markers were used to type seven clinical avian isolates and 22 environmental isolates of a ... | 2013 | 22977077 |
| isolation of the volatile fraction from apium graveolens l. (apiaceae) by supercritical carbon dioxide extraction and hydrodistillation: chemical composition and antifungal activity. | apium graveolens l. (wild celery), belonging to the family of apiaceae, is a scaposus hemicryptophyte. instead, the cultivate plant is an annual or biennial herb widely used as a spice and seasoning in food. a broad range of biological activities have been attributed to a. graveolens. these include antimicrobial activity, larvicidal activity, hepatoprotective activity, nematicidal and mosquito repellent potential and antihyperlipidaemic properties.in this study, the authors compare the compositi ... | 2013 | 22974401 |
| azole-resistant invasive aspergillosis in a patient with acute myeloid leukaemia in germany. | we report the first culture-proven case of invasive aspergillosis (ia) caused by azole-resistant aspergillus fumigatus in a patient with acute myeloid leukaemia in germany. ia presented as breakthrough infection under posaconazole prophylaxis. analysis of the resistance mechanism revealed the tr/l98h mutation in the cyp51a gene, which indicates an environmental origin of the strain. this case underscores the need for monitoring azole resistance in aspergillus spp. and for routine susceptibility ... | 2012 | 22971327 |
| what is the current place of azoles in allergic bronchopulmonary aspergillosis and severe asthma with fungal sensitization. | evaluation of: chishimba l, niven rm, cooley j, denning dw. voriconazole and posaconazole improve asthma severity in allergic bronchopulmonary aspergillosis and severe asthma with fungal sensitization. j. asthma 49(4), 423-433 (2012). allergic bronchopulmonary aspergillosis (abpa) is a pulmonary disorder caused by hypersensitivity to aspergillus fumigatus that usually complicates the disease course of patients with asthma and cystic fibrosis. oral corticosteroids are currently the treatment of c ... | 2012 | 22971061 |
| characterization of the velvet regulators in aspergillus fumigatus. | fungal development and secondary metabolism is intimately associated via activities of the fungi-specific velvet family proteins. here we characterize the four velvet regulators in the opportunistic human pathogen aspergillus fumigatus. the deletion of afuvosa, afuvea and afuvelb causes hyperactive asexual development (conidiation) and precocious and elevated accumulation of afubrla during developmental progression. moreover, the absence of afuvosa, afuvea or afuvelb results in the abundant form ... | 2012 | 22970834 |
| effects of aspergillus fumigatus colonization on lung function in cystic fibrosis. | aspergillus fumigatus is frequently isolated from cystic fibrosis (cf) patients and is notorious for its role in the debilitating condition of allergic bronchopulmonary aspergillosis (abpa). although cf patients suffer from perpetual microorganism-related lung disease, it is unclear whether a. fumigatus colonization has a role in causing accelerated lung function decline and whether intervention is necessary. | 2012 | 22965276 |
| chitin synthases with a myosin motor-like domain control the resistance of aspergillus fumigatus to echinocandins. | aspergillus fumigatus has two chitin synthases (csma and csmb) with a myosin motor-like domain (mmd) arranged in a head-to-head configuration. to understand the function of these chitin synthases, single and double csm mutant strains were constructed and analyzed. although there was a slight reduction in mycelial growth of the mutants, the total chitin synthase activity and the cell wall chitin content were similar in the mycelium of all of the mutants and the parental strain. in the conidia, ch ... | 2012 | 22964252 |
| expression and secretion of aspergillus fumigatus proteases are regulated in response to different protein substrates. | the ubiquitous filamentous fungus aspergillus fumigatus secretes a number of allergens with protease activity and has been linked to a variety of allergic conditions such as severe asthma with fungal sensitization (safs) and allergic bronchopulmonary aspergillosis (abpa). however, it is unclear which allergen proteases are being secreted during fungal invasion and whether the local biological environment regulates their expression. understanding the dynamic expression of allergen proteases durin ... | 2012 | 22954343 |
| clinical features of allergic bronchopulmonary aspergillosis in korea. | allergic bronchopulmonary aspergillosis (abpa) is a complex disease, triggered by a hypersensitivity reaction to the allergen aspergillus fumigatus. this disease occurs frequently in patients with cystic fibrosis and severe asthma in western countries, with a prevalence of 2%-15%. however, there have been only a few case reports in korea. we investigated the clinical and immunological features of patients with abpa. ten adult patients diagnosed with abpa, according to greenberger's criteria, wer ... | 2012 | 22950037 |
| identification of the urinary metabolites of glionitrin a in rats using ultra-performance liquid chromatography combined with quadrupole time-of-flight mass spectrometry. | glionitrin a (gn a) is a new diketopiperazine disulfide with an aromatic nitro group, which is isolated from the coculture of an aspergillus fumigatus fungal strain and a sphingomonas bacterial strain. after intravenous administration of gn a in rats, 13 urinary metabolites of gn a were identified using ultra-performance liquid chromatography/quadrupole time-of-flight mass spectroscopy (uplc-qtop-ms) analysis in conjunction with data processing programs such as metabolynx™ and massfragnent™. red ... | 2012 | 22947415 |
| [severe contact lens-associated fungal keratitis]. | a 23-year-old man presented with severe contact lens-associated keratitis and descemetocele with pre-existing drug therapy. after 1 week of intensive antibiotic treatment aspergillus fumigatus was identified. despite adjusted antimycotic treatment a corneal perforation occurred. due to peripheral scleral infiltration the cornea was primarily closed with a double layer amniotic membrane in order to avoid a sclerokeratoplasty with a bad prognosis. after 2 weeks the peripheral corneal situation sta ... | 2012 | 22940808 |
| nucleotide oligomerization domain 2 contributes to the innate immune response in thce cells stimulated by aspergillus fumigatus conidia. | to investigate the expression of nucleotide oligomerization domain 2 (nod2) in the immortalized human corneal epithelial cell line (thce), and its role in the innate immune response triggered by inactive aspergillus fumigatus (af) conidia. | 2012 | 22937496 |
| the β-glucan receptor dectin-1 promotes lung immunopathology during fungal allergy via il-22. | sensitization to fungi, such as the mold aspergillus fumigatus, is increasingly becoming linked with asthma severity. we have previously shown that lung responses generated via the β-glucan receptor dectin-1 are required for lung defense during acute, invasive a. fumigatus infection. unexpectedly, in an allergic model of chronic lung exposure to live a. fumigatus conidia, β-glucan recognition via dectin-1 led to the induction of multiple proallergic (muc5ac, clca3, ccl17, ccl22, and il-33) and p ... | 2012 | 22933634 |
| aspergillus tracheobronchitis: report of 8 cases and review of the literature. | aspergillus tracheobronchitis (at) is an infrequent but severe form of invasive pulmonary aspergillosis in which the fungal infection is entirely or predominantly confined to the tracheobronchial tree. we reviewed 8 cases of at diagnosed in our tertiary care center during an 18-year period, as well as 148 cases previously reported in the english literature from 1985 to july 2011. the demographic, clinical, imaging, bronchoscopic, and outcome characteristics of every eligible patient were excerpt ... | 2012 | 22932790 |
| [effects of chronic aspergillus fumigatus exposure on the expression of mucin 5ac in the airways of asthmatic rats]. | to explore the effects of chronic aspergillus fumigatus (af) exposure on the expression of mucin 5ac (muc5ac) in the airways of asthmatic rats. | 2012 | 22931723 |