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prevalence and antimicrobial resistance of salmonella enteritidis and other salmonellas in the eggs and egg-storing trays from retail markets of coimbatore, south india.a study was conducted to determine the incidence of salmonella enterica serovar enteritidis and other salmonella serovars on eggshell, egg contents and on egg-storing trays. a total of 492 eggs and 82 egg-storing trays were examined over a period of 1 year from different retail outlets of a residential area of coimbatore city, south india. salmonella contamination was recorded in 38 of 492 (7.7%) eggs out of which 29 was in eggshell (5.9%) and 9 in egg contents (1.8%). around 7.5% of the egg-sto ...200616943017
comparison of pathogenic and spoilage bacterial levels on refrigerated poultry parts following treatment with trisodium phosphate.this study was undertaken to determine whether trisodium phosphate decontamination of poultry could give a competitive advantage to pathogens and increase microbiological risk to consumers. chicken legs were co-inoculated with similar concentrations of pathogenic (salmonella enteritidis or listeria monocytogenes) and spoilage (pseudomonas fluorescens or brochothrix thermosphacta) bacteria. samples were dipped in tsp (12%, 15 min) or were non-treated (control). microbiological analyses were carri ...200616943004
[algorithm for prediction of reconvalescent salmonellosis carriage].of total of 181 patients with salmonella infection were examined by clinical and laboratory methods. as many as 124 indices characterizing biological properties of salmonellae, a large intestine microbiocenosis status, systemic and local immunity, were investigated as well. among these indices, the most revealing signs were defined to prognosticate the reconvalescent carrier state. conclusion was made on the prevalence of immune mechanisms in the development of reconvalescent salmonella carriage ...200616941887
adaptive resistance and differential protein expression of salmonella enterica serovar enteritidis biofilms exposed to benzalkonium chloride.the development of adaptive resistance of salmonella enterica serovar enteritidis atcc 4931 biofilms following exposure to benzalkonium chloride (bc) either continuously (1 microg ml(-1)) or intermittently (10 microg ml(-1) for 10 min daily) was examined. biofilms adapted to bc over a 144-h period could survive a normally lethal bc challenge (500 microg ml(-1) for 10 min) and then regrow, as determined by increases in biofilm thickness, total biomass, and the ratio of the viable biomass to the n ...200616940079
differential regulation of porcine beta-defensins 1 and 2 upon salmonella infection in the intestinal epithelial cell line ipi-2i.intestinal epithelial cells represent the first line of defence against pathogenic bacteria in the lumen of the gut. besides acting as a physical barrier, epithelial cells orchestrate the immune response through the production of several innate immune mediator molecules including beta-defensins. here, we establish the porcine intestinal cell line ipi-2i as a new model system to test the regulation of porcine beta-defensins 1 and 2. gene expression of both defensins was highly upregulated by foet ...200616938353
some probiotic properties of vaginal lactobacilli isolated from healthy women.lactobacilli have long been considered to be protective flora in the vagina. in this study, samples were taken from the lateral vaginal wall of 19 healthy women, and 58 strains of lactobacillus spp. were isolated from 10 of these women. the identification results revealed that lactobacillus gasseri was the predominant lactobacilli in healthy women. the amounts of lactic acid and hydrogen peroxide (h(2)o(2)) produced by lactobacillus spp. were determined. the vaginal ph levels induced by lactobac ...200616936344
cross-reactive cellular and humoral immune responses to salmonella enterica serovars typhimurium and enteritidis are associated with protection to heterologous re-challenge.chickens infected with salmonella enterica serovars typhimurium (st) and enteritidis (se) still represent a major source of human food poisoning via consumption of contaminated meat and eggs. vaccination represents a sustainable approach to control salmonella in the chicken and the serovar specificity of immunity has the potential to impact on the need for multivalent vaccines. the issue of cross-reactive immune responses and cross-serovar protection was examined in these experiments. cellular a ...200616935350
detection of egg yolk antibodies reflecting salmonella enteritidis infections using a surface plasmon resonance biosensor.a surface plasmon resonance (spr) biosensor assay was developed on the basis of a lipopolysaccharide antigen of salmonella enterica serovar enteritidis (s. enterica serovar enteritidis) to detect egg yolk antibodies against s. enterica serovar enteritidis. this biosensor assay was compared to two commercial elisa kits based on lps antigen and flagellar antigen. a number of 163 egg yolk and combined egg white and yolk samples from chickens experimentally infected with s. enterica serovar enteriti ...200616926019
survival of salmonella enteritidis phage type 30 on inoculated almonds stored at -20, 4, 23, and 35 degrees c.to evaluate the survival of salmonella on raw almond surfaces, whole almond kernels were inoculated with salmonella enteritidis phage type (pt) 30 collected from a 24-h broth culture or by scraping cells from an agar lawn. kernels inoculated with lawn-collected cells to 8, 5, 3, and 1 log cfu per almond after a 24-h drying period were stored for 161 days at 23 +/- 3 degrees c. calculated rates of reduction were similar for the four inoculum levels (0.22, 0.28, 0.29, and 0.22 log cfu/month, respe ...200616924909
salmonella enteritidis septic arthritis after allogeneic peripheral blood stem cell transplantation. 200616923588
ctx-m-producing salmonella spp. in hong kong: an emerging problem.two salmonella enterica serovar typhimurium isolates and one s. enterica serovar enteritidis isolate that were resistant to 4 microg cefotaxime ml-1 and produced ctx-m-type extended-spectrum beta-lactamases (esbls) were characterized from patients in hong kong during 2003-2004. the s. typhimurium strain isolated in 2003 produced a ctx-m-9 esbl and harboured a blactx-m-9 gene that was associated with a class i integron-containing gene cassette and orf513 similar to those of in60. the second s. ty ...200616914655
estimation of endotoxin in infected bile from patients with biliary diseases.diseases of the biliary tract can get complicated by infection. endotoxin may theoretically be responsible for damage to the gall bladder due to its numerous pathophysiological effects. the aim of the present study was to detect and semi-quantitate the amount of endotoxin present in the bacteriologically positive bile samples and to correlate the endotoxin levels with the clinical profile of the patients. one hundred patients with gall bladder diseases and with infected bile constituted the popu ...200616910056
are happy chickens safer chickens? poultry welfare and disease susceptibility.1. contaminated chicken meat remains an internationally important vehicle for human infection with salmonella and campylobacter spp. in addition, the last 20 years has seen an international pandemic of human salmonellosis caused by the contamination of eggs with salmonella enteritidis. 2. it has been a long held scientific view that campylobacter spp. and most, if not all of the common zoonotic salmonella, are essentially commensal in chickens. they usually form part of the gut flora and contami ...200616905463
cyclosporine inhibits endotoxin-induced vasodilation of isolated rat resistance arterioles.an isolated arteriole fails to dilate in response to endotoxin unless a segment of aorta is included in the perfusion system. the unknown substance released by the aorta after exposure to endotoxin is dependent upon the nf-kappab pathway and induces inducible nitric oxide synthase (inos) in the arteriole. the purpose of this study was to determine if cyclosporine a (csa) that inhibits both nf-kappab and inos would prevent the vasodilatory response to endotoxin.200616904696
the effect of feed deprivation on tissue invasion by salmonella enteritidis.a tissue culture procedure was utilized to compare tissue cell invasion by salmonella enteritidis from molted and full feed hens. three identical trials were performed in which 80-wk-old active laying hens were divided into 2 groups of 6 birds each. the molted hen group was subjected to a 14-d feed withdrawal, and the full-fed hen group was administered a standard layer ration. after feed treatment, crop, ileum, cecum, and ovary (small and large yellow follicles removed) were collected, rinsed i ...200616903461
heat-treatment of bovine colostrum. ii: effects of heating duration on pathogen viability and immunoglobulin g.batches (30-l) of first-milking bovine colostrum, inoculated with mycoplasma bovis (10(8) cfu/ml), listeria monocytogenes (10(6) cfu/ml), escherichia coli o157:h7 (10(6) cfu/ml), salmonella enteritidis (10(6) cfu/ml), and mycobacterium avium subsp. paratuberculosis (map; 10(3) cfu/ml), were heat-treated at 60 degrees c for 120 min in a commercial on-farm batch pasteurizer system. duplicate 50-ml subsamples of colostrum were collected at 15-min intervals throughout the heat-treatment process for ...200616899682
antibacterial activity of the metabolic by-products of a veillonella species and bacteroides fragilis.a veillonella species and bacteroides fragilis were isolated from the cecal contents of adult chickens. when growth on an agar medium supplemented with 0.4% glucose and adjusted to ph 6.5, mixed cultures containing veillonella and b. fragilis inhibited the growth of salmonella typhimurium; salmonella enteritidis, escherichia coli 0157:h7 and pseudomonas aeruginosa. decreasing the glucose concentration of the agar decreased the inhibitory activity of the mixed culture. mixed cultures grown on aga ...199516887516
septic arthritis in patients with systemic lupus erythematosus: salmonella and nonsalmonella infections compared.to assess the clinical characteristics and outcome of systemic lupus erythematosus (sle) with septic arthritis.200616887469
development of an ultra rapid and simple multiplex polymerase chain reaction technique for detection of salmonella typhi.to make a rapid and definite diagnosis of salmonella enteritis, using an ultra rapid multiplex polymerase chain reaction (pcr) detection method for major salmonella serotypes, such as salmonella typhi, salmonella typhimurium and salmonella havana.200616883440
prevention of salmonella cross-contamination in an oilmeal manufacturing plant.the mechanisms of salmonella contamination in an oilmeal plant were investigated and the basic data were collected in order to achieve control of salmonella in oilmeal.200616882155
two separate outbreaks of salmonella enteritidis phage type 14b food poisoning linked to the consumption of the same type of frozen food.to ascertain, using a combination of epidemiological, environmental and microbiological methods of investigation, a possible link between two outbreaks of salmonella food poisoning.200616879847
cluster of invasive salmonellosis cases in a federal prison in colorado.we report a cluster of 3 cases of invasive salmonellosis without gastroenteritis among prisoners, in the absence of a gastroenteritis outbreak. raw foods illicitly taken from the prison kitchen and improperly stored and prepared were possible sources of infection. we describe the investigation of this cluster and discuss the unique implications of invasive, nongastroenteritis salmonellosis in the prison setting, both for clinicians and for public health agencies that seek to control infections i ...200616877102
antimicrobial activity of essential oils on salmonella enteritidis, escherichia coli, and listeria innocua in fruit juices.the antimicrobial properties of essential oils (eos) and their derivatives have been known for years. however, the information published about the minimal effective concentration of eos against microorganisms in fruit juices is scarce. in this study, both mic and mbc of six eos (lemongrass, cinnamon, geraniol, palmarosa, or benzaldehyde) against salmonella enteritidis, escherichia coli, and listeria innocua were determined by the agar and broth dilution methods, respectively. all of the six eos ...200616865889
influence of eggshell condensation on eggshell penetration and whole egg contamination with salmonella enterica serovar enteritidis.shells of agar-filled and whole eggs were inoculated with 10(3) to 10(4) cfu of salmonella enterica serovar enteritidis per eggshell. the agar-filled eggs were used to study bacterial eggshell penetration, and the whole egg results were used to characterize contamination of the egg contents. in each group, half of the eggs were stored for 21 days at 20 degrees c and 60% relative humidity (rh), and the other half was stored for 24 h at 6 degrees c and then for 20 days at 20 degrees c. the latter ...200616865883
protection conferred by a live salmonella enteritidis vaccine against fowl typhoid in laying hens.fowl typhoid is under control in poultry farms of developed countries, but it still endemically subsists in commercial laying hen farms of some countries. it has been demonstrated that salmonella live vaccines can elicit cross-immunity against members of the same kauffmann-white scheme serogroup. in this work, we explored the protection conferred by tad salmonella vac e, a live salmonella enterica serovar enteritidis vaccine, against fowl typhoid. three groups of laying hens were vaccinated with ...200616863082
preliminary evaluation of the use of the sefa fimbrial gene to elicit immune response against salmonella enterica serotype enteritidis in chickens.in the last 2 decades, the prevalence of salmonella enterica serotype enteritidis (salmonella enteritidis) has dramatically increased worldwide, becoming the leading cause of food-borne illnesses and an important public health issue. many studies have suggested the role of the sef14 fimbrial protein in the adhesion of salmonella enteritidis to the host. in the present study, the sefa gene, which encodes the main subunit of the sef14 fimbrial protein, was cloned into a temperature-sensitive expre ...200616863065
[bacterial contamination in an egg-breaking factory and its control].from january to november, 2003, bacterial contamination was surveyed in a small egg-breaking factory that produced non pasteurized liquid egg. test egg samples were taken at various stages of the egg processing operation. salmonella enteritidis was isolated from liquid egg yolk and liquid egg white on october, but was not found in any other samples (50 liquid egg samples, 21 containers and 94 attached production facilities and gloves). the data suggest that the contamination rate (3.8%) is lower ...200616862990
antibacterial activity of bioconverted eicosapentaenoic (epa) and docosahexaenoic acid (dha) against foodborne pathogenic bacteria.the antimicrobial activity of bioconversion extracts of epa and dha against a range of foodborne pathogenic bacteria was investigated. the bioconverted epa and dha exhibited antibacterial activities against four gram-positive bacteria, bacillus subtilis, listeria monocytogenes, staphylococcus aureus (atcc 6538) and s. aureus (kctc 1916) and seven gram-negative bacteria, enterobacter aerogenes, escherichia coli, e. coli o157:h7, e. coli o157:h7 (human), pseudomonas aeruginosa, salmonella enteriti ...200716860896
enhancement of mice susceptibility to infection with listeria monocytogenes by the treatment of morphine.the effect of morphine on the susceptibility of balb/c mice to diarrheagenic escherichia coli, shigella flexneri, listeria monocytogenes, salmonella enteritidis, yersinia enterocolitica, was examined via the intraperitoneal inoculation. morphine treatment increased the susceptibility to s. enteritidis and l. monocytogenes, resulting in bacteremia and central nervous system (cns) invasion (for l. monocytogenes), while the infection with other bacteria did not show the systemic dissemination in th ...200616858145
structure of a capsular polysaccharide isolated from salmonella enteritidis.salmonella enteritidis is a food-borne enteric human pathogen that can form a complex protective extracellular matrix. we describe here a component of this matrix which is distinct from other known salmonella extracellular polysaccharides such as cellulose and colanic acid. we have used glycosyl composition and linkage analysis, as well as 1d and 2d nmr spectroscopy to determine the structure of this polysaccharide. we propose that the primary saccharide in the s. enteritidis capsule has a branc ...200616857179
oral immunisation of laying hens with the live vaccine strains of tad salmonella vac e and tad salmonella vac t reduces internal egg contamination with salmonella enteritidis.eggs are a major source of human infections with salmonella. therefore controlling egg contamination in laying hen flocks is one of the main targets for control programmes. a study was carried out to assess the effect of oral vaccination with tad salmonella vac e, tad salmonella vac t and with both vaccines tad salmonella vac e and tad salmonella vac t, on colonization of the reproductive tract and internal egg contamination of laying hens with salmonella enteritidis. three groups of 30 laying h ...200616842890
salmonella enterica serotype enteritidis and eggs: a national epidemic in the united states.beginning in the 1970s, the incidence of salmonella enterica serotype enteritidis (se) infection and the number of related outbreaks in the united states has increased dramatically. by 1994, se was the most commonly reported salmonella serotype, with an incidence of >10 laboratory-confirmed infections per 100,000 population in the northeast. intensive epidemiologic and laboratory investigations identified shell eggs as the major vehicle for se infection in humans, and that the eggs had been inte ...200616838242
mechanical ventilation affects alveolar fibrinolysis in lps-induced lung injury.the aim of the present study was to determine the effects of mechanical ventilation on alveolar fibrin turnover in lipopolysaccharide (lps)-induced lung injury. in a randomised controlled trial, sprague-dawley rats (n = 61) were allocated to three ventilation groups after intratracheal lps (salmonella enteritidis) instillations. group i animals were subjected to 16 cmh(2)o positive inspiratory pressure (pip) and 5 cmh(2)o positive end-expiratory pressure (peep); group ii animals to 26 cmh(2)o pi ...200616837499
consumer-phase salmonella enterica serovar enteritidis risk assessment for egg-containing food products.we describe a one-dimensional probabilistic model of the role of domestic food handling behaviors on salmonellosis risk associated with the consumption of eggs and egg-containing foods. six categories of egg-containing foods were defined based on the amount of egg contained in the food, whether eggs are pooled, and the degree of cooking practiced by consumers. we used bootstrap simulation to quantify uncertainty in risk estimates due to sampling error, and sensitivity analysis to identify key so ...200616834632
effect of various combinations of alfalfa and standard layer diet on susceptibility of laying hens to salmonella enteritidis during forced molt.feed deprivation is commonly used by the poultry industry to induce molting and stimulate multiple egg-laying cycles. however, feed deprivation has been observed experimentally to increase susceptibility of poultry to salmonella infections. previous studies indicated that alfalfa was efficacious in reducing salmonella; the present investigation was designed to evaluate the efficacy of combined alfalfa and layer diets on salmonella colonization. leghorn hens over 50 wk of age were divided into 12 ...200616830850
[persistence of the salmonella enteritidis recombinant strain with expressed hbc antigen in mice and macrophage culture].the behavior of s. enteritidis recombinant strain e-23/pkdna(3.1) with hbc antigen expressed by the eukaryotic promoter (dna vaccine) in the body of mice after their infection per rectum and in the culture of macrophages was studied. the expression of hbc was shown to lead to the loss of the capacity of salmonellae for persistence in parenteral lymphoid tissue and for inducing the formation of anti-hbc antibodies. the study of the interaction of salmonellae with the macrophage culture revealed t ...200616830589
submandibular abscess caused by salmonella.salmonella species represent uncommon causative agents in focal infections of the head and neck. this article presents a rare case of salmonella submandibular abscess in a 59-year-old patient with diabetes mellitus, chronic hepatitis and liver cirrhosis. the patient was successfully treated with drainage of the abscess and oral administration of quinolone. an immunocompromised condition can be a critical predisposing factor for salmonella submandibular abscess.200616829030
unveiling contamination sources and dissemination routes of salmonella sp. in pigs at a portuguese slaughterhouse through macrorestriction profiling by pulsed-field gel electrophoresis.sixty-nine isolates of salmonella sp. isolated from the ileum, tonsils, carcass and mandibular and ileocolic lymph nodes of individual pigs slaughtered for consumption in one abattoir were analyzed using serotyping and macrorestriction profiling by pulsed-field gel electrophoresis (rflp-pfge), in order to identify clonal relationships. xbai macrorestriction was able to distinguish 18 genotypes among the eight identified serotypes: salmonella typhimurium (4 genotypes), salmonella rissen (3), salm ...200616828912
eggshell factors influencing eggshell penetration and whole egg contamination by different bacteria, including salmonella enteritidis.trans-shell infection routes and whole egg contamination of 7 selected bacterial strains; staphylococcus warneri, acinetobacter baumannii, alcaligenes sp., serratia marcescens, carnobacterium sp., pseudomonas sp. and salmonella enteritidis, recovered from egg contents, were studied. the first objective was to correlate bacterial eggshell penetration with various eggshell characteristics and bacterial strains. an agar approach was used to assess the eggshell penetration. the second objective was ...200616822571
fluorescence microscopy of nacl-stressed, elongated salmonella and listeria cells reveals the presence of septa in filaments.the cell morphology of salmonella enteritidis and listeria monocytogenes after the application of stress was examined. cells were stressed by plating the bacteria on tryptone soya agar (tsa) plates, with 5-10% nacl. the plates were subsequently incubated for 6 days at 25 degrees c. finally, the cells were harvested and subjected to different fluorescent probes in order to visualize the possible presence of septa in elongated cells. use of the stain 4',6-diamidino-2-phenylindole (dapi), which is ...200616806551
substantial reduction of human salmonella enteritidis infections in luxembourg in 2005. 200616801710
successful reduction of human salmonella enteritidis infection in england and wales. 200516794281
circulating gamma delta t cells in response to salmonella enterica serovar enteritidis exposure in chickens.gammadelta t cells are considered crucial to the outcome of various infectious diseases. the present study was undertaken to characterize gammadelta (t-cell receptor 1(+) [tcr1(+)]) t cells phenotypically and functionally in avian immune response. day-old chicks were orally immunized with salmonella enterica serovar enteritidis live vaccine or s. enterica serovar enteritidis wild-type strain and infected using the s. enterica serovar enteritidis wild-type strain on day 44 of life. between days 3 ...200616790770
effect of refrigeration on in vitro penetration of salmonella enteritidis through the egg yolk membrane.internally contaminated eggs have been implicated as leading sources of transmission of salmonella enteritidis (se) to humans. although se is not often deposited inside the nutrient-rich yolks of naturally contaminated eggs, penetration through the vitelline membrane to reach the yolk contents could result in rapid bacterial multiplication. in previous studies, such penetration has been observed occasionally at warm temperatures during experiments with in vitro egg contamination models. the pres ...200616786867
surveillance of foodborne disease outbreaks associated with consumption of eggs and egg products: spain, 2002-2003. 200516783100
a model of salmonella infection within industrial house hens.salmonella is one of the major sources of toxi-infection in humans. incidences of human salmonellosis have greatly increased over the past 20 years and this can largely be attributed to epidemics of salmonella enteritidis phage type 4 within poultry. the main concern with this bacterium is the existence of silent carriers, i.e. animals harbouring s. enteritidis without expressing any visible symptoms. in this article, we formulate a model for s. enteritidis transmission in hen houses, considerin ...200616780891
[a case of cervical abscess caused by salmonella enteritidis after intestinal infection].salmonella enteritidis often causes food poisoning. in this study, an extremely rare case of cervical abscess caused by s. enteritidis is reported. in january 2003, a 44-year-old man visited our hospital with swelling of the left submandibular region. he had been suffering from a severe diabetic condition but had neglected to seek medical attention. an incision was made at the abscess to drain the pus from which only s. enteritidis was isolated. this finding led to the discovery that he had suff ...200616780136
epididymo-orchitis and testicular abscess caused by salmonella enteritidis in immunocompromised patients in kuwait.to report relatively uncommon presentations of epididymo-orchitis and testicular abscess caused by salmonella spp. in 2 immunocompromised patients.200616763400
breed effect on early cytokine mrna expression in spleen and cecum of chickens with and without salmonella enteritidis infection.we examined mrna expression of 11 genes: bak, bcl-x, interferon [ifn]-gamma, interleukin [il]-1beta, il-6, il-10, il-12alpha, il-12beta, il-18, cxcli2 [il-8/caf], and a mip family chemokine, ccli2, in the spleen and cecum of day-old chicks after oral inoculation with salmonella enteritidis (se) or medium. three distinct chicken breeds (broiler, fayoumi, and leghorn) were evaluated for mrna expression levels at 2 and 18h post-inoculation using quantitative reverse transcriptase-polymerase chain r ...200716762413
[incidence of reactive arthritis and other musculoskeletal sequelae following a foodborne outbreak of salmonella enteritidis phago type 14 b infection].we intended to estimate the prevalence of reactive arthritis (rea) and other musculoskeletal sequelae after a foodborne outbreak of salmonella enteritidis phago type 14 b in a banquet in castellón in june 5th, 2004.200616756904
re-assessment of risk factors for sporadic salmonella serotype enteritidis infections: a case-control study in five foodnet sites, 2002-2003.active surveillance for laboratory-confirmed salmonella serotype enteritidis (se) infection revealed a decline in incidence in the 1990s, followed by an increase starting in 2000. we sought to determine if the fluctuation in se incidence could be explained by changes in foodborne sources of infection. we conducted a population-based case-control study of sporadic se infection in five of the foodborne diseases active surveillance network (foodnet) sites during a 12-month period in 2002-2003. a to ...200716756692
comparison of heterophil functions of modern commercial and wild-type rio grande turkeys.the purpose of the present study was to measure any functional differences in peripheral blood heterophils isolated from a commercial turkey line to wild-type rio grande turkeys. the phagocytosis of salmonella enteritidis, oxidative burst (oxb) and degranulation (dgr) were used as parameters of heterophil functional efficiency in these studies. blood was collected and heterophils isolated from each line of turkeys at days 4, 7, and 14 post-hatch. on days 4 and 7 post-hatch, heterophils from rio ...200616753613
comparison of methods of extracting salmonella enterica serovar enteritidis dna from environmental substrates and quantification of organisms by using a general internal procedural control.this paper compares five commercially available dna extraction methods with respect to dna extraction efficiency of salmonella enterica serovar enteritidis from soil, manure, and compost and uses an escherichia coli strain harboring a plasmid expressing green fluorescent protein as a general internal procedural control. inclusion of this general internal procedural control permitted more accurate quantification of extraction and amplification of s. enterica serovar enteritidis in these samples a ...200616751492
[ventriculitis due to salmonella enteritidis]. 200616750083
antimicrobial resistance in non-typhoidal salmonella from human sources, spain, 2001-2003.to determine the current state of antimicrobial resistance among non-typhoidal salmonella strains isolated from humans in spain.200616735424
assessment of cleaning and disinfection in salmonella-contaminated poultry layer houses using qualitative and semi-quantitative culture techniques.salmonella infection of laying flocks in the uk is predominantly a problem of the persistent contamination of layer houses and associated wildlife vectors by salmonella enteritidis. methods for its control and elimination include effective cleaning and disinfection of layer houses between flocks, and it is important to be able to measure the success of such decontamination. a method for the environmental detection and semi-quantitative enumeration of salmonellae was used and compared with a stan ...200616730925
isolation and characterization of four antibacterial peptides from bovine hemoglobin.peptic digestion of bovine hemoglobin at low degree of hydrolysis yields several intermediate peptide fractions after separation by reversed phase hplc exhibiting antibacterial activity against micrococcus luteus a270, listeria innocua, escherichia coli, and salmonella enteritidis. from these fractions, four new antibacterial peptides were isolated and analyzed by esi-ms/ms. three of these peptides correspond to fragments of the alpha-chain of bovine hemoglobin: alpha107-141, alpha137-141, and a ...200616730859
acute renal failure with encephalopathy following salmonella enteritidis infection. 200616721591
multiplication and motility of salmonella enterica serovars enteritidis, infantis, and montevideo in in vitro contamination models of eggs.the invasive ability of salmonella enterica serovars enteritidis, infantis, and montevideo in eggs was examined. strains of these serovars originating from egg contents, laying chicken houses, and human patients were experimentally inoculated (0.1-ml dose containing 78 to 178 cells) onto the vitelline membrane of eggs collected from specific-pathogen-free chickens and incubated at 25 degrees c. the test strains were detected in 25 of 138 yolk contents by day 6, indicating the penetration of salm ...200616715797
graft replacement and muscle wrap for infected aneurysm of thoracic aorta.a 68-year-old man was admitted with high fever and enlargement of the distal aortic arch on chest radiography. computed tomography showed a huge proximal descending thoracic aortic aneurysm. graft replacement of the proximal descending thoracic aorta was performed on an emergency basis, and a pectoralis major muscle flap was wrapped around the graft. salmonella enteritidis was detected in the resected tissue. the patient recovered well, with no signs of infection.200616714706
analyses of five gallinacin genes and the salmonella enterica serovar enteritidis response in poultry.gallinacins in poultry are functional equivalents of mammalian beta-defensins, which constitute an integral component of the innate immune system. salmonella enterica serovar enteritidis is a gram-negative bacterium that negatively affects both human and animal health. to analyze the association of genetic variations of the gallinacin genes with the phenotypic response to s. enterica serovar enteritidis, an f1 population of chickens was created by crossing four outbred broiler sires to dams of t ...200616714567
large outbreak of salmonella enteritidis in north east london. 200516702640
salmonella carrier-state in hens: study of host resistance by a gene expression approach.salmonellosis is one of the main causes of food-borne poisoning due to the consumption of contaminated poultry products. in the flocks, salmonella is able to persist in the digestive tract of birds for weeks without triggering any symptom. in order to identify molecules and genes involved in the mechanism of host resistance to intestinal carrier-state, two different inbred lines of laying hens were orally inoculated with salmonella enteritidis. bacterial colonization and host gene expression wer ...200616702014
identification of salmonella spp. isolates from chicken abattoirs by multiplex-pcr.the present study was carried out to report the occurrence salmonella spp., salmonella enteritidis, and salmonella typhimurium in chicken abattoirs. samples of feces; feathers; scald, evisceration, and chiller water; and rinse water of non-eviscerated, eviscerated, and chilled carcass were collected from six chicken abattoirs. salmonella isolates were identified by a multiplex-pcr using three sets of primers targeting the inva, pefa, and sefa gene sequences from salmonella spp., s. typhimurium a ...200616701750
differences in gene expression levels and in enzymatic qualities account for the uneven contribution of superoxide dismutases sodci and sodcii to pathogenicity in salmonella enterica.most salmonella enterica serovars produce two periplasmic [cu,zn] superoxide dismutases, sodci, which is prophage encoded, and sodcii, encoded by a conserved chromosomal gene. both enzymes were proposed to enhance salmonella virulence by protecting bacteria against products of macrophage oxidative burst. however, we previously found sodci, but not sodcii, to play a role during mouse infection by s. enterica serovar typhimurium. here we have extended these findings to another serovar of epidemiol ...200616697686
[antiphospholipid syndrome in a patient with salmonella enteritidis bacteremia]. 200616680859
rapid and simple detection of food poisoning bacteria by bead assay with a microfluidic chip-based system.a rapid bead assay for detecting pathogenic bacteria with a simple microfluidic chip-based system was developed. five oligonucleotide probes corresponding to the 16s rrna of the targeted bacteria were coupled covalently to fluorescent beads. four species of bacteria (escherichia coli, salmonella enterica subsp. enterica serovar enteritidis, yersinia enterocolitica, and bacillus cereus) were used as representative food-borne pathogenic bacteria. the rnas extracted from pure cultures of these micr ...200616678290
salmonella challenge affects the antibody isotype profile of bile in hens differing in metabolic efficiency.gel precipitation reactions determined antibody isotypes in bile from hens differing in dietary efficiency. ouchterlony double diffusion employing alpha-chain specific goat-anti-chicken iga, rabbit anti-chicken igg, goat anti-chicken igm, black turtle bean (btb), and jacalin lectins as precipitating reagents detected bile iga, igg, and igm from salmonella exposed and nonexposed hens. the iga was present in 1 of 3 forms designated by reagent and frequency: igab (precipitated by btb lectin) 100%; ...200616673763
salmonella serovars in the herpetofauna of indiana county, pennsylvania.herpetofaunal salmonella enterica serovars have not been fully examined in any u.s. region. thirty-three salmonella serovars were isolated from 156 samples from 34 species, all within indiana county, pennsylvania. results suggest that herpetofaunas could potentially pose a threat to humans. further understanding of salmonella in herpetofaunas may prevent future human cases.200616672533
mannan-binding lectin activates c3 and the alternative complement pathway without involvement of c2.lectin pathway activation of c3 is known to involve target recognition by mannan-binding lectin (mbl) or ficolins and generation of classical pathway c3 convertase via cleavage of c4 and c2 by mbl-associated serine protease 2 (masp-2). we investigated c3 activation in c2-deficient human sera and in sera with other defined defects of complement to assess other mechanisms through which mbl might recruit complement. the capacity of serum to support c3 deposition was examined by elisa using microtit ...200616670774
the anti-helicobacter pylori flavones in a brazilian plant, hyptis fasciculata, and the activity of methoxyflavones.two known flavonoids were isolated from a tropical medicinal plant, hyptis fasciculata (labiatae), found in brazil. flavonoids were identified as cirsilineol (1) and cirsimaritin (2) by spectroscopic means and were exhibited potent antibacterial activity against helicobacter pylori, and cirsilineol (1) had weak antibacterial activity against escherichia coli and salmonella enteritidis. following up on the relationship between anti-h. pylori activity and flavonoids with methoxy groups, several me ...200616651742
survival characteristics of salmonella enterica serovar enteritidis in chicken egg albumen.salmonella enterica serovar enteritidis (se) is a major foodborne pathogen primarily causing human infection through contaminated chicken eggs. to understand how se survives in chicken egg albumen, we systematically and quantitatively analysed the survival properties of se in egg albumen and identified factors affecting its survival. survival assays of se in egg indicate that egg albumen restricted the growth of se. a major factor that controlled se's growth in egg albumen was iron restriction, ...200616650332
salmonella enterica serovar enteritidis colonization of the chicken caecum requires the hila regulatory protein.invasion of salmonella into intestinal epithelial cells is believed to be essential for the pathogenesis of salmonella infections. invasion is mediated by genes located on the salmonella pathogenicity island i (spi-1), which are needed for assembling a type three secretion system, that mediates injection of bacterial proteins into the cytosol of epithelial cells, resulting in cytoskeletal rearrangements and as a consequence invasion. hila is the key regulator of the salmonella pathogenicity isla ...200616647227
immunity induced with a salmonella enterica serovar enteritidis live vaccine is regulated by th1-cell-dependent cellular and humoral effector mechanisms in susceptible balb/c mice.the objective of this study was to characterize the immune response induced by a live attenuated salmonella enteritidis (se; ade(-)/his(-)) vaccine using an intraperitoneal immunization/challenge model in susceptible wild-type and cytokine-deficient balb/c mice. in wild-type mice, inoculation of the se live vaccine induced a protective immune response characterized by both cellular (production of interleukin(il)-12 and interferon(ifn)-gamma, granuloma formation in liver and spleen, dth response) ...200616647169
salmonellosis outbreak among factory workers--huizhou, guangdong province, china, july 2004.during july 9--14, 2004, an outbreak of gastroenteritis occurred among workers at an electronics factory in huizhou, guangdong province, china; 199 cases were reported. a case-control investigation was initiated to identify the agent and the mode of transmission.200616645581
[salmonella prosthetic knee septic arthritis successful retention of the prosthesis with prolonged suppressive therapy].a 75 years old woman suffered from salmonella enteritidis infected prosthetic knee joint septic arthritis. maintenance of the prosthesis was achieved by surgical drainage and debridement of the joint followed by 3 weeks of intravenous ceftriaxone and 3 months of oral ciprofloxacin therapy.200616642625
clonal structure and variation in virulence of salmonella enteritidis isolated from mice, chickens, and humans.the clonal analysis of a diverse collection of salmonella enteritidis indicates that most strains belong to a single multilocus genotype (i.e., et-3) regardless of phage type, geographic origin, or time of isolation that spanned over 2 decades (1978 to 2004). attachment and invasion assays, however, indicate that, among et-3 isolates, there is a distinct invasive bacterial subpopulation that is more readily recovered from eggs and clinical cases in humans than from chicken cecal samples. these o ...200616640300
risk factors for salmonella enteritidis and typhimurium (dt104 and non-dt104) infections in the netherlands: predominant roles for raw eggs in enteritidis and sandboxes in typhimurium infections.since 1996 salmonella typhimurium dt104 salmonellosis has increased in the netherlands. this prompted a case-control study of risk factors for salmonellosis to inform transmission routes for this phage type. cases were laboratory-confirmed patients with a salmonella infection and controls were selected from population registries by frequency matching for age, sex, degree of urbanization and season. cases and controls received a questionnaire on risk factors. of the 1171 cases, 573 (49%) responde ...200616638166
effect of environmental stresses on antibody-based detection of escherichia coli o157:h7, salmonella enterica serotype enteritidis and listeria monocytogenes.to study the reaction patterns of selected antibodies to escherichia coli o157:h7, salmonella enterica serotype enteritidis and listeria monocytogenes cells exposed to various environmental stresses.200616630002
growth of salmonella enteritidis phage type 30 in almond hull and shell slurries and survival in drying almond hulls.traceback investigation of a 2000 to 2001 outbreak of salmonellosis associated with consumption of raw almonds led to isolation of the outbreak strain salmonella enterica serovar enteritidis phage type (pt) 30 on three geographically linked almond farms. interviews with these growers revealed that significant rain fell during the 2000 harvest when many almonds were drying on the ground. the objectives of this study were to document weather conditions during the 2000 harvest, determine the potent ...200616629010
salmonella enterica serovar enteritidis producing a tem-52 beta-lactamase: first report in spain.tem-52 is an extended-spectrum beta-lactamase (esbl) that is being increasingly reported in enterobacteria. in spain, tem-52 had been reported before only in escherichia coli. here we report the first finding of a tem-52 esbl in salmonella in spain, associated to a tn3 transposon.200616626913
influence of slc11a1 on the outcome of salmonella enterica serovar enteritidis infection in mice is associated with th polarization.genetic analyses identified ses1 as a significant quantitative trait locus influencing the carrier state of 129s6 mice following a sublethal challenge with salmonella enterica serovar enteritidis. previous studies have determined that slc11a1 was an excellent candidate gene for ses1. kinetics of infection in 129s6 mice and slc11a1-deficient (129s6-slc11a1(tm1mcg)) mice demonstrated that the wild-type allele of slc11a1 contributed to the s. enterica serovar enteritidis carrier state as early as 7 ...200616622216
functional characterization of the fimh adhesin from salmonella enterica serovar enteritidis.salmonella enterica serovar enteritidis has emerged during the last 20 years as the major causative agent of food-borne gastroenteritis in humans and as the major infectious agent on poultry farms, replacing salmonella enterica serovar typhimurium as the dominant pathogenic serovar. because adhesion to gut tissues and colonization of the alimentary tract, mediated in large part by the fimh adhesins located on type 1 fimbriae, is an important stage in the pathogenesis of both serovars, the bindin ...200616622051
cutaneous leukocytoclastic vasculitis in a child with interleukin-12 receptor beta-1 deficiency.we report a patient with complete interleukin-12 receptor beta-1 deficiency associated with cutaneous leukocytoclastic vasculitis. the patient experienced bacille calmette guérin, mycobacterium chelonae, and salmonella enteritidis infection. vasculitis affecting both small arteries and postcapillary venules due to deposition of immune complexes was probably caused by s. enteritidis and/or m. chelonae infection.200616615980
antimicrobial resistance in salmonella enteritidis from foods involved in human salmonellosis outbreaks in southern brazil.the antimicrobial resistance of salmonella enteritidis (n = 79) isolated from foods involved in human salmonellosis outbreaks in southern brazil during the period of 2001 to 2002 was analysed. the isolates were individually tested using the disc diffusion method against 10 antimicrobial agents. most isolates were susceptible to all drugs tested. no s. enteritidis isolates were resistant to sulfamethoxazole/trimethoprim or sulfazotrim and only one was resistant to chloramphenicol. the predominant ...200616608125
genomic diversity within phage types of salmonella enterica ssp. enterica serotypes enteritidis and typhimurium.the diversity among 1354 strains of salmonella enterica subsp. enterica, serotype enteritidis (n = 847) and typhimurium (n = 507) isolated in finland in 1991-2002 (n = 608) and in 2003 (n = 746) were studied. the former strains were studied retrospectively by phage typing and pulsed-field gel electrophoresis (pfge) harmonized in the european salm-gene project. the latter strains were studied prospectively, and the results correlated to their antimicrobial susceptibility and association with trav ...200616602985
selection of potentially probiotic lactobacillus strains towards their inhibitory activity against poultry enteropathogenic bacteria.lactobacilli were isolated from chicken gastrointestinal tract and examined for their potentially probiotic properties towards their inhibitory activity against poultry enteropathogenic bacteria. biochemical tests, its-pcr and cell wall protein analysis were used to characterize the lactobacillus isolates. the identification of isolated lactobacillus strains based on phenotypic properties was not always satisfactory. its-pcr together with protein profile were found to be helpful in strain identi ...200516599299
heterophil cytokine mrna profiles from genetically distinct lines of chickens with differential heterophil-mediated innate immune responses.previously we demonstrated that increased in-vitro heterophil function translates to increased in-vivo resistance to salmonella enteritidis infections in broilers (line a > b). heterophils produce cytokines and modulate acute protection against salmonella in neonatal poultry. we hypothesized that heterophils from s. enteritidis-resistant chickens produce an up-regulated pro-inflammatory cytokine/chemokine response compared with s. enteritidis-susceptible chickens. in this study, heterophils were ...200616595301
invasive multidrug-resistant non-typhoidal salmonella infections in africa: zoonotic or anthroponotic transmission?in africa, multidrug-resistant non-typhoidal salmonellae (nts) are one of the leading causes of morbidity and high mortality in children under 5 years of age, second in importance only to pneumococcal disease. the authors studied nts isolates from paediatric admissions at two hospitals in nairobi, kenya, and followed the index cases to their homes, where rectal swabs and stools from parents and siblings, and from animals in close contact, were obtained. the majority of nts obtained from cases we ...200616585646
molecular analysis of salmonella enteritidis and typhimurium clinical and food isolates by pulsed-field gel electrophoresis in bogotá, colombia.in 1997, the microbiology group of the colombian instituto nacional de salud set up a surveillance program with the national public health laboratories to monitor the principal etiological agents responsible for acute diarrheal disease. the main goal of this study was to determine the xbai dna digestion patterns of clinical and food salmonella spp. isolates recovered in bogotá from 1997 to 2002, and related them to the susceptibility patterns to antimicrobial agents. two hundred and twenty-four ...200616584312
[rapid identification of riemerella anatipestifer on the basis of specific pcr amplifying 16s rdna].riemerella anatipestifer (ra) infection is the main disease causing severe losses in duck production. because ra is characterized more by the absence than by the presence of specific phenotypic properties and different scholar had the different results of biochemical detection, it can't always be identified quickly and correctly only by the phenotypic properties or biochemical characteristics. the research object was to develop a species specific pcr method for ra detection. because of the conse ...200616579457
evaluation of isopathic treatment of salmonella enteritidis in poultry.salmonellosis is a common problem worldwide in commercially reared poultry. it is associated with human salmonellosis. no fully satisfactory method of control is available.200616569625
isolation of a soluble resistance-enhancing factor from mycobacterium phlei.fox, alfred e. (warner-lambert research institute, morris plains, n.j.), joachim anschel, george l. evans, raam r. mohan, and benjamin s. schwartz. isolation of a soluble resistance-enhancing factor from mycobacterium phlei. j. bacteriol. 92:285-290. 1966.-extraction of a crude cell wall preparation from mycobacterium phlei with 20% urea yielded a fraction which induced a state of enhanced resistance to microbial challenge. the resulting soluble extract, after removal of the urea, represented a ...196616562108
experimental salmonellosis vi. in vitro transfer of cellular immunity of mouse mononuclear phagocytes.saito, kazuko (gunma university, maebashi, japan), and susumu mitsuhashi. experimental salmonellosis. vi. in vitro transfer of cellular immunity of mouse mononuclear phagocytes. j. bacteriol. 90:629-634. 1965.-a culture medium of the mononuclear phagocytes (monocytes) of mice immunized with live vaccine of salmonella enteritidis contains the transfer agent (ta) of cellular immunity from immune to non-immune monocytes. the ta is of ribonucleic acid nature, is nondialyzable through cellophane, and ...196516562059
antibiotic interrelationships among the enteric group of bacteria. 194716561421
the effect of the reaction of the medium on the characteristics of bacteria: i. general presentation of the problem, and results obtained with bacillus coli-communior, salmonella enteritidis and pseudomonas pyocyanea. 193316559648
enhancement of bacterial infections in mice by newcastle disease virus.this report describes an attempt to define the factors which incite secondary bacterial pneumonias. groups of mice were given bacteria intraperitoneally and, at various intervals, newcastle disease virus intravenously. there was an increase in the number of deaths and in the rates of death in these groups, when compared with a control group which was given only bacteria. these results were obtained with streptococcus pneumoniae (diplococcus pneumoniae), pseudomonas aeruginosa, and salmonella ent ...197116558000
oral immunization in experimental salmonellosis iii. behavior of virulent and temperature-sensitive mutant strains in the intestinal tissues of rats.infection of rats via a peyer's patch has been used as a means of studying the behavior of salmonella enteritidis in intestinal tissues. the course of infection in the peyer's patch and draining mesenteric lymph node is characterized by multiplication of the organism over a period of 4 days followed by a gradual decline in numbers; the organism also passes to the liver and spleen and may be isolated from these organs as well as the intestinal tissues for at least 4 weeks. temperature-sensitive m ...197016557819
oral immunization in experimental salmonellosis ii. characteristics of the immune response to temperature-sensitive mutants given by oral and parenteral routes.a temperature-sensitive mutant of salmonella enteritidis, selected because of its inability to proliferate normally at 37 c, has been used as a living vaccine in mice. when given parenterally or orally, it confers a high degree of resistance against otherwise lethal s. enteritidis infections given intraperitoneally or by mouth. in contrast to most other effective living salmonella vaccines, the temperature-sensitive mutant survives for only short periods in mouse tissues. although the vaccine pr ...197016557818
subcellular localization of salmonella enteritidis endotoxin in liver and spleen of mice and rats.salmonella enteritidis(14)c-endotoxin was recovered predominantly from the nuclear and mitochondrial subcellular fractions of livers and spleens of mice and rats, 3.5 hr and 3 days after intravenous administration. of the recovered radioactivity, 10 to 20% was present in the liver mitochondrial fraction as high-molecular-weight, biologically active material, suggesting the presence of intact endotoxin. autoradiographic studies demonstrated nuclear and cytoplasmic labeling in the liver and at lea ...197016557755
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