Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| immunodeficiency scoring index to predict poor outcomes in hematopoietic cell transplant recipients with rsv infections. | we developed an immunodeficiency scoring index for respiratory syncytial virus (isi-rsv) infection, based on a cohort of 237 allogeneic hematopoietic cell transplant (allo-hct) recipients, that can predict the risk of progression to lower respiratory tract infection (lrti) and rsv-associated mortality. a weighted index was calculated using adjusted hazard ratios for immunodeficiency markers. based on the isi-rsv (range, 0-12), patients were stratified into low (0-2), moderate (3-6), and high (7- ... | 2014 | 24700783 |
| recombinant bovine respiratory syncytial virus with deletion of the sh gene induces increased apoptosis and pro-inflammatory cytokines in vitro, and is attenuated and induces protective immunity in calves. | bovine respiratory syncytial virus (brsv) causes inflammation and obstruction of the small airways, leading to severe respiratory disease in young calves. the virus is closely related to human (h)rsv, a major cause of bronchiolitis and pneumonia in young children. the ability to manipulate the genome of rsv has provided opportunities for the development of stable, live attenuated rsv vaccines. the role of the sh protein in the pathogenesis of brsv was evaluated in vitro and in vivo using a recom ... | 2014 | 24700100 |
| relationships between a(h1n1)pdm09 influenza infection and infections with other respiratory viruses. | a(h1n1)pdm09, a new influenza pandemic virus emerged in 2009. the a(h1n1)pdm09 infection had several unique characteristics which included rapid transmissibility and high morbidity in obese individuals, pregnant women and individuals suffering from chronic diseases. | 2014 | 24698156 |
| [differentiation of influenza (flu) type a, type b, and respiratory syncytial virus (rsv) by quicknavi™-flu+rsv]. | because it is not easy to differentiate influenza virus (flu) from rs virus (rsv) just by clinical symptoms, to accurately diagnose those viruses in conjunction with patient's clinical symptoms, rapid diagnostic kits has been used separately for each of those viruses. in our new study, we have developed a new rapid diagnostic kit, quicknavi™-flu+rsv. the kit can detect flu a, flu b, and rsv antigens with a single sample collection and an assay. total of 2,873 cases (including nasopharyngeal swab ... | 2014 | 24694242 |
| authors' response re: clarifying costs and benefits of respiratory syncytial virus immunoprophylaxis. | 2014 | 24692038 | |
| clarifying costs and benefits of respiratory syncytial virus immunoprophylaxis. | 2014 | 24692037 | |
| examining strain diversity and phylogeography in relation to an unusual epidemic pattern of respiratory syncytial virus (rsv) in a long-term refugee camp in kenya. | a recent longitudinal study in the dadaab refugee camp near the kenya-somalia border identified unusual biannual respiratory syncytial virus (rsv) epidemics. we characterized the genetic variability of the associated rsv strains to determine if viral diversity contributed to this unusual epidemic pattern. | 2014 | 24690157 |
| the influence of virus infections on the course of copd. | chronic obstructive pulmonary disease (copd) is extensively influenced by viral infections. the mechanisms of how viral agents affect the pathogenesis and prognosis of copd are numerous. in general, patients with infectious exacerbations are characterized by longer hospitalization periods and greater impairment of several lung function parameters than those with non-infectious exacerbations. prodromal, clinical, and outcome parameters fail to distinguish virally from non-virally induced illnesse ... | 2012 | 24688763 |
| spectrum of viral infections in patients with cystic fibrosis. | this review explores the extensive influence of viral infections leading to chronic deterioration of lung function in patients with cystic fibrosis (cf). the mechanisms how viral agents affect the pathogenesis as well as the inflammatory and immune response of cf are discussed. viral infections of the upper and lower respiratory tract due to viruses in cf patients and methods for diagnosis of respiratory viruses are described in detail. the importance of respiratory and non-respiratory viral age ... | 2012 | 24688762 |
| comparison of levels and duration of detection of antibodies to bovine viral diarrhea virus 1, bovine viral diarrhea virus 2, bovine respiratory syncytial virus, bovine herpesvirus 1, and bovine parainfluenza virus 3 in calves fed maternal colostrum or a colostrum-replacement product. | colostrum-replacement products are an alternative to provide passive immunity to neonatal calves; however, their ability to provide adequate levels of antibodies recognizing respiratory viruses has not been described. the objective of this study was to compare the serum levels of igg at 2 d of age and the duration of detection of antibodies to bovine viral diarrhea virus 1 (bvdv-1), bovine viral diarrhea virus 2 (bvdv-2), bovine respiratory syncytial virus (brsv), bovine herpesvirus 1 (bhv-1), a ... | 2014 | 24688168 |
| distinct regulation of tonsillar immune response in virus infection. | the relationships between tonsillar immune responses, and viral infection and allergy are incompletely known. | 2014 | 24684577 |
| depletion of circulating regulatory t cells during severe respiratory syncytial virus infection in young children. | 2014 | 24684360 | |
| real-time light scattering tracking of gold nanoparticles- bioconjugated respiratory syncytial virus infecting hep-2 cells. | real-time tracking of virus invasion is crucial for understanding viral infection mechanism, which, however, needs simple and efficient labeling chemistry with improved signal-to-noise ratio. for that purpose, herein we investigated the invasion dynamics of respiratory syncytial virus (rsv) through dark-field microscopic imaging (idfm) technique by using au nanoparticles (aunps) as light scattering labels. rsv, a ubiquitous, non-segmented, pleiomorphic and negative-sense rna virus, is an importa ... | 2014 | 24681709 |
| comparison of urinary tract infection rates among 2- to 12-month-old febrile infants with rsv infections using 1999 and 2011 aap diagnostic criteria. | infants with rsv infections have been found to have a clinically significant rate of urinary tract infections (utis). the american academy of pediatrics (aap) published a revised clinical practice guideline on utis in 2011, which includes major changes in diagnostic criteria for utis. past research has been done using previous diagnostic criteria. the objective of the study is to assess the rate of utis in febrile infants with respiratory syncytial virus (rsv) infections according to the 2011 re ... | 2014 | 24681546 |
| the human metapneumovirus small hydrophobic protein has properties consistent with those of a viroporin and can modulate viral fusogenic activity. | human metapneumovirus (hmpv) encodes three glycoproteins: the glycoprotein, which plays a role in glycosaminoglycan binding, the fusion (f) protein, which is necessary and sufficient for both viral binding to the target cell and fusion between the cellular plasma membrane and the viral membrane, and the small hydrophobic (sh) protein, whose function is unclear. the sh protein of the closely related respiratory syncytial virus has been suggested to function as a viroporin, as it forms oligomeric ... | 2014 | 24672047 |
| the thr205 phosphorylation site within respiratory syncytial virus matrix (m) protein modulates m oligomerization and virus production. | human respiratory syncytial virus (rsv) is the most common cause of bronchiolitis and pneumonia in infants and the elderly worldwide; however, there is no licensed rsv vaccine or effective drug treatment available. the rsv matrix (m) protein plays key roles in virus assembly and budding, but the protein interactions that govern budding of infectious virus are not known. in this study, we focus on m protein and identify a key phosphorylation site (thr205) in m that is critical for rsv infectious ... | 2014 | 24672034 |
| animal model of respiratory syncytial virus: cd8+ t cells cause a cytokine storm that is chemically tractable by sphingosine-1-phosphate 1 receptor agonist therapy. | the cytokine storm is an intensified, dysregulated, tissue-injurious inflammatory response driven by cytokine and immune cell components. the cytokine storm during influenza virus infection, whereby the amplified innate immune response is primarily responsible for pulmonary damage, has been well characterized. now we describe a novel event where virus-specific t cells induce a cytokine storm. the paramyxovirus pneumonia virus of mice (pvm) is a model of human respiratory syncytial virus (hrsv). ... | 2014 | 24672024 |
| lung function by impulse oscillometry at age 5-7 years after bronchiolitis at age 0-6 months. | viral bronchiolitis in infancy has been associated with increased bronchial reactivity and reduced lung function in later childhood and even in adulthood. however, lung function at preschool age is less studied, mainly due to technical difficulties. the purpose of the study was to evaluate lung function and bronchial reactivity at preschool age in children who were hospitalized for bronchiolitis in early infancy. | 2015 | 24668616 |
| viral coinfection in childhood respiratory tract infections. | the introduction of molecular techniques has enabled better understanding of the etiology of respiratory tract infections in children. the objective of the study was to analyze viral coinfection and its relationship to clinical severity. | 2015 | 24666712 |
| retrospective serology study of respiratory virus infections in captive great apes. | great apes are extremely sensitive to infections with human respiratory viruses. in this study, we retrospectively analyzed sera from captive chimpanzees, gorillas and orang-utans. more than 1000 sera (403 chimpanzee, 77 gorilla, and 535 orang-utan sera) were analyzed for antibodies to the human respiratory viruses rsv (respiratory syncytial virus, hmpv (human metapneumovirus), h1n1 and h3n2 influenza a viruses, and influenza b virus. in all ape species high seroprevalences were found for rsv, h ... | 2014 | 24662675 |
| performance of a rapid multi-analyte 2-photon excitation assay in children with acute respiratory infection. | the purpose of this study is to evaluate the diagnostic performance of the novel 2-photon excitation-based maripoc© assay (arcdia laboratories, turku, finland) for antigen detection of respiratory viruses versus real-time polymerase chain reaction (pcr). the maripoc assay and 2 multiplex real-time pcr techniques were performed on nasopharyngeal samples from pediatric patients with suspicion of acute respiratory infection admitted to a children's hospital in spain during october 2011 to january 2 ... | 2014 | 24661686 |
| in vitro antiviral activity of a series of wild berry fruit extracts against representatives of picorna-, orthomyxo- and paramyxoviridae. | wild berry species are known to exhibit a wide range of pharmacological activities. they have long been traditionally applied for their antiseptic, antimicrobial, cardioprotective and antioxidant properties. the aim of the present study is to reveal the potential for selective antiviral activity of total methanol extracts, as well as that of the anthocyanins and the non-anthocyanins from the following wild berries picked in bulgaria: strawberry (fragaria vesca l.) and raspberry (rubus idaeus l.) ... | 2014 | 24660461 |
| a new diterpenoid from the aerial parts of andrographis paniculata. | one new and seven known diterpenoids were isolated from the aerial parts of andrographis paniculata, and their structures elucidated on the basis of spectroscopic and spectrometric analysis, as well as chemical methods. the in vitro antiviral activities of these compounds against respiratory syncytial virus (rsv) were assayed. | 2014 | 24660450 |
| axl receptor blockade ameliorates pulmonary pathology resulting from primary viral infection and viral exacerbation of asthma. | viruses use tyro3, axl, and mertk (tam) receptor tyrosine kinases to infect and modulate the immune properties of various cell types, which led us to investigate whether tam receptor activation affected primary viral infection and viral exacerbation of asthma in experimental models. in these lung-specific models, we observed that axl was the most abundantly induced tam receptor protein. during primary respiratory syncytial virus (rsv) infection, anti-axl mab treatment significantly increased the ... | 2014 | 24659691 |
| evaluation of fast technology analysis (fta) cards as an improved method for specimen collection and shipment targeting viruses associated with bovine respiratory disease complex. | in order to improve the analytic quality of respiratory specimens collected from cattle for nucleic acid-based diagnosis, a study was undertaken to verify realtime pcr efficiency of specimens collected and stabilized on fta cards™, filter paper which is treated chemically. nucleic acids collected using fta cards without the need for a cold-chain or special liquid media handling provided realtime pcr results consistent (96.8% agreement, kappa 0.923 [95% ci=0.89-0.96]) with the same specimens coll ... | 2014 | 24657552 |
| fatal broncho-pneumonia of an infant with arthrogryposis multiplex congenita (amc). | arthrogryposis multiplex congenita (amc) is a heterogenous group of disorders characterized by multiple joint contractures with an estimated frequency of 1 in 3000-5100 livebirths. the authors present a case of an 11-month-old infant with amc who died due to severe pneumonia. the deceased had no significant anomaly except for the stated orthpaediatric deformities, and the long-term outcome was expected to be positive. the only remarkable past medical history was respiratory syncytial virus (rs) ... | 2014 | 24657039 |
| small interfering rnas targeted to interleukin-4 and respiratory syncytial virus reduce airway inflammation in a mouse model of virus-induced asthma exacerbation. | asthma exacerbations are caused primarily by viral infections. antisense and small interfering rna (sirna) technologies have gained attention as potential antiasthma and antiviral approaches. in this study we analyzed whether gene silencing of interleukin (il)-4 expression and respiratory syncytial virus (rsv) replication by rna interference is able to suppress allergen- and virus-induced responses in a mouse model of virus-induced asthma exacerbation. knockdown efficacy of il-4 sirna molecules ... | 2014 | 24655063 |
| prevalence and molecular characterization of human metapneumovirus in influenza a negative sample in thailand. | human metapneumovirus (hmpv) causes respiratory tract infection in influenza-like illness. the role of hmpv infections in all age groups in thailand has not yet been investigated. thus, the objective of this study was to determine prevalence of hmpv infection in all age groups in thailand during 2011. | 2014 | 24652781 |
| another case of respiratory syncytial virus-related limbic encephalitis. | 2014 | 24652531 | |
| macrophages are required for dendritic cell uptake of respiratory syncytial virus from an infected epithelium. | we have previously shown that the respiratory syncytial virus [rsv] can productively infect monocyte derived dendritic cells [modc] and remain dormant within the same cells for prolonged periods. it is therefore possible that infected dendritic cells act as a reservoir within the airways of individuals between annual epidemics. in the present study we explored the possibility that sub-epithelial dcs can be infected with rsv from differentiated bronchial epithelium and that in turn rsv from dcs c ... | 2014 | 24651119 |
| elevated th17 response in infants undergoing respiratory viral infection. | il-17 and t-helper (th)17 cells contribute to viral airway pathology in human newborns. because umbilical cord blood t cells fail to differentiate toward the th17 lineage in the presence of autologous antigen-presenting cells, we asked whether th17 cells are present in young infants that experience respiratory viral infection. to this end, we analyzed tracheal aspirate samples from infant patients suffering from acute respiratory syncytial virus (rsv) infection and healthy infant controls. acute ... | 2014 | 24650560 |
| alpha/beta interferon receptor signaling amplifies early proinflammatory cytokine production in the lung during respiratory syncytial virus infection. | type i interferons (ifns) are produced early upon virus infection and signal through the alpha/beta interferon (ifn-α/β) receptor (ifnar) to induce genes that encode proteins important for limiting viral replication and directing immune responses. to investigate the extent to which type i ifns play a role in the local regulation of inflammation in the airways, we examined their importance in early lung responses to infection with respiratory syncytial virus (rsv). ifnar1-deficient (ifnar1(-/-)) ... | 2014 | 24648449 |
| infants 1-90 days old hospitalized with human rhinovirus infection. | human rhinovirus (hrv) is a common cause of respiratory illness in children. the impact of hrv infection on 1- to 90-day-old infants is unclear. we hypothesized that hrv infection would be clinically similar to respiratory syncytial virus (rsv) infection in the hospitalized infants. | 2014 | 24648198 |
| [development and validation of the real-time pcr assay for the identification of viral agents causing acute respiratory infections in humans]. | the real time pcr assay targeting influenza a and b virus, 5 subtypes of influenza a virus (seasonal h1n1, pandemic h1n1 (2009), seasonal h3n2, pathogenic for human subtypes of avian influenza h5 and h7), respiratory syncytial virus, and adenovirus was developed. the analytical sensitivity of the developed assay was 1 x 10(3) genome equivalents per ml. the diagnostic sensitivity of the method was 1 x l0(3)-10(4) viral particles per ml. experiments with human dna/cdna and viral cdna showed a mark ... | 2013 | 24645276 |
| sucrose stabilization of respiratory syncytial virus (rsv) during nebulization and experimental infection. | respiratory syncytial virus (rsv) is a common respiratory pathogen that can cause severe pneumonia. in vivo studies of rsv can be difficult due to variation in viral infection and disease severity in some animal models. factors that may contribute to the variation are decreases in viral titer due to preparation and storage and method of virus administration. nebulization is one method of rsv administration that provides even distribution of virus to all lung lobes; however, the exact quantity of ... | 2014 | 24642084 |
| [nosocomial respiratory syncytial virus bronchiolitis in preterm infants: characteristics of the course, treatment and prevention in clinical practice]. | to study the clinical, laboratory and radiological features of the course of nosocomial bronchiolitis respiratory syncytial viral (rsv) etiology and effectiveness of the therapy in preterm infants in the neonatal hospital conditions. | 2013 | 24640732 |
| influenza and respiratory syncytial viral infections in malaysia: demographic and clinical perspective. | respiratory infections represent a major public health problem worldwide. the study aimed to determine the prevalence of respiratory syncytial and influenza virus infections and analyzed in respect to demography and clinical perspective. methods : the specimens were processed by cell culture and immunofluorescent assay (ifa) and real-time reverse transcriptase-pcr (rrt-pcr) for detection of respiratory viruses. results : out of 505 specimens 189 (37.8%) were positive, in which rsv was positive i ... | 2014 | 24639853 |
| viral infections in asthma and copd. | airway viral infections are associated with the pathogenesis of asthma and copd. it has been argued that respiratory syncytial virus (rsv) infection in infancy is a probable causal factor in the development of pediatric asthma. rsv infections tend to induce th2-biased immune responses in the host airways. rsv infection, atopy, and low pulmonary function in neonates may work synergistically toward the development of pediatric asthma. human rhinovirus (hrv) is a representative virus associated wit ... | 2014 | 24636264 |
| respiratory syncytial virus and palivizumab: where are we in 2014? | 2014 | 24634681 | |
| gene sequence variability of the three surface proteins of human respiratory syncytial virus (hrsv) in texas. | human respiratory syncytial virus (hrsv) has three surface glycoproteins: small hydrophobic (sh), attachment (g) and fusion (f), encoded by three consecutive genes (sh-g-f). a 270-nt fragment of the g gene is used to genotype hrsv isolates. this study genotyped and investigated the variability of the gene and amino acid sequences of the three surface proteins of hrsv strains collected from 1987 to 2005 from one center. sixty original clinical isolates and 5 prototype strains were analyzed. seque ... | 2014 | 24625544 |
| respiratory syncytial virus disease in preterm infants in the u.s. born at 32-35 weeks gestation not receiving immunoprophylaxis. | the respiratory syncytial virus (rsv) respiratory events among preterm infants outcomes and risk tracking (report) study evaluated rsv disease burden in u.s. preterm infants 32-35 weeks gestational age (wga) not receiving rsv prophylaxis. | 2014 | 24622396 |
| the role of influenza and parainfluenza infections in nasopharyngeal pneumococcal acquisition among young children. | animal models suggest that influenza infection favors nasopharyngeal acquisition of pneumococci. we assessed this relationship with influenza and other respiratory viruses in young children. | 2014 | 24621951 |
| oral ribavirin therapy for respiratory syncytial virus infections in moderately to severely immunocompromised patients. | respiratory syncytial virus (rsv) infections may be fatal in immunocompromised patients. aerosolized ribavirin is used for treatment, but it is very costly, teratogenic, and inconvenient. we aimed to assess the outcome of oral ribavirin treatment, with or without intravenous immunoglobulin (ivig), for rsv infections in moderately to severely immunocompromised patients. | 2014 | 24621016 |
| respiratory virus infections among children in south china. | acute respiratory tract infection is an important cause of morbidity and mortality with a worldwide disease burden. this study aimed to determine the prevalence and clinical characteristics of children with viral-induced acute respiratory tract infection, in southern china. nasopharyngeal aspirate samples from 1,980 pediatric patients with suspected acute respiratory tract infection, and 82 samples from healthy subject controls were collected for routine examination at the second affiliated hosp ... | 2014 | 24619492 |
| the evolving role of the pediatric nurse practitioner in hospital medicine. | this program evaluation sought to compare cost and pediatric patient outcomes among a pediatric nurse practitioner (pnp) hospitalist team, a combined pnp/doctor of medicine (md) team, and 2 resident teams without pnps. | 2014 | 24616251 |
| infection of differentiated airway epithelial cells from caprine lungs by viruses of the bovine respiratory disease complex. | bovine respiratory syncytial virus (brsv), bovine parainfluenza virus type 3 (bpiv3) and bovine herpesvirus type 1 (bhv-1) are important pathogens associated with the bovine respiratory disease complex (brdc). non-bovine ruminants such as goats may also be infected and serve as a virus reservoir to be considered in the development of control strategies. to evaluate the susceptibility of caprine airway epithelial cells to infection by viruses of brdc, we established a culture system for different ... | 2014 | 24612951 |
| viral etiology of influenza-like illnesses in huizhou, china, from 2011 to 2013. | little information is available on the etiology and prevalence of viruses other than influenza viruses causing influenza-like illnesses (ilis) in china. this study was conducted for simultaneous detection and identification of 14 respiratory viruses in huizhou using real-time pcr. in total, viruses were detected in 48.66 % of ili patient samples, in which influenza virus (19.98 %) was the most commonly detected, followed by rhinovirus (7.46 %), human coronaviruses (3.63 %), human metapneumovirus ... | 2014 | 24610554 |
| nosocomial transmission of respiratory syncytial virus in an outpatient cancer center. | respiratory syncytial virus (rsv) outbreaks in inpatient settings are associated with poor outcomes in cancer patients. the use of molecular epidemiology to document rsv transmission in the outpatient setting has not been well described. we performed a retrospective cohort study of 2 nosocomial outbreaks of rsv at the seattle cancer care alliance. subjects included patients seen at the seattle cancer care alliance with rsv detected in 2 outbreaks in 2007-2008 and 2012 and all employees with resp ... | 2014 | 24607551 |
| viral aetiology and clinical outcomes in hospitalised infants presenting with respiratory distress. | to determine the prevalence of various types of viruses in infants hospitalised due to respiratory distress, compare molecular diagnostic tests and evaluate symptom severity. | 2014 | 24606114 |
| [the treatment of influenza and acute respiratory viral infections]. | acute respiratory viral infections (arvi) and influenza take a leading place in the structure of the general morbidity of the population in most countries of the world. of etiotropic treatment of influenza and other arvi include a wide spectrum of antiviral drugs, among which are the drugs with a restricted spectrum, basically used in influenza, and a general spectrum used in influenza and other arvi as a prophylactic or therapeutic intention. at present time, there is a widespread antiviral dru ... | 2013 | 24605619 |
| improving influenza surveillance in portuguese preschool children by parents' report. | influenza surveillance is usually based on nationally organized sentinel networks of physicians and on hospital reports. this study aimed to test a different report system, based on parents' phone contact to the research team and in home collection of samples by a dedicated team. the identification of influenza and other respiratory viruses in children who attended a hospital emergency department was also recorded. real-time pcr and reverse transcription pcr were performed for influenza a and b, ... | 2014 | 24599798 |
| epidemiological changes of respiratory syncytial virus (rsv) infections in israel. | rsv is the leading cause of lower respiratory-tract infections in infants and therefore demands in-depth epidemiological characterization. we investigated here the distribution of rsv types in israel between the years 2005-2012. clinical samples were collected from 11,018 patients hospitalized due to respiratory illnesses and were evaluated for the presence of various respiratory viruses, including rsv a and rsv b. until 2008, each year was characterized by the presence of one dominant type of r ... | 2014 | 24594694 |
| characteristic systemic cytokine responses in children with human bocavirus-positive lower respiratory tract infection. | to investigate systemic cytokine responses in human bocavirus (hbov)-associated lower respiratory tract infection, serum cytokine profiles were analyzed in hbov positive-children (n=14) using multiplex immunoassay. concentrations of tnf-a, il-2, il-5 and il-8 on admission were significantly different from those of respiratory syncytial virus-positive children (n=28). this unique cytokine response might partly explain some characteristic clinical features of hbov-associated respiratory infection. | 2014 | 24593186 |
| respiratory syncytial virus infections enhance cigarette smoke induced copd in mice. | respiratory syncytial viral (rsv) infections are a frequent cause of chronic obstructive pulmonary disease (copd) exacerbations, which are a major factor in disease progression and mortality. rsv is able to evade antiviral defenses to persist in the lungs of copd patients. though rsv infection has been identified in copd, its contribution to cigarette smoke-induced airway inflammation and lung tissue destruction has not been established. here we examine the long-term effects of cigarette smoke e ... | 2014 | 24587397 |
| using mathematical transmission modelling to investigate drivers of respiratory syncytial virus seasonality in children in the philippines. | we used a mathematical transmission model to estimate when ecological drivers of respiratory syncytial virus (rsv) transmissibility would need to act in order to produce the observed seasonality of rsv in the philippines. we estimated that a seasonal peak in transmissibility would need to occur approximately 51 days prior to the observed peak in rsv cases (range 49 to 67 days). we then compared this estimated seasonal pattern of transmissibility to the seasonal patterns of possible ecological dr ... | 2014 | 24587222 |
| population-based incidence of severe acute respiratory virus infections among children aged <5 years in rural bangladesh, june-october 2010. | better understanding the etiology-specific incidence of severe acute respiratory infections (saris) in resource-poor, rural settings will help further develop and prioritize prevention strategies. to address this gap in knowledge, we conducted a longitudinal study to estimate the incidence of saris among children in rural bangladesh. | 2014 | 24587163 |
| evaluation of the advansure™ real-time rt-pcr compared with culture and seeplex rv15 for simultaneous detection of respiratory viruses. | recently, advansure™ kit based on multiplex real-time pcr was developed for simultaneous detection of 14 respiratory viruses (rvs). we compared the performance of advansure with those of seeplex® rv 15 ace and culture by determining their sensitivities and specificities against a composite reference standard. four hundred thirty-seven respiratory samples were tested by modified shell vial culture method, rv 15 ace, and advansure. one hundred fourteen samples (26.2%) out of 437 samples were posit ... | 2014 | 24582583 |
| the clinical utility of a near patient care rapid microarray-based diagnostic test for influenza and respiratory syncytial virus infections in the pediatric setting. | we evaluated the potential clinical utility of an automated near patient molecular assay verigene respiratory virus plus (rv+) and rapid immunochromatographic antigen tests (riat) in the pediatric setting for diagnosis of influenza and respiratory syncytial virus infections when testing was performed by the pediatrician seeing the patient. overall, with respect to influenza virus, sensitivity and specificity for riat were 70.8% and 100%, respectively, compared to 100% and 96.2%, respectively, fo ... | 2014 | 24582577 |
| prevalence of respiratory viral infection in children hospitalized for acute lower respiratory tract diseases, and association of rhinovirus and influenza virus with asthma exacerbations. | in this study, we aimed to investigate the prevalence of year-round respiratory viral infection in children with lower respiratory tract infection (lrti) and the relationship between respiratory viral infection and allergen sensitization in exacerbating asthma. | 2014 | 24578714 |
| characterization of human coronavirus oc43 and human coronavirus nl63 infections among hospitalized children <5 years of age. | multiplex molecular assays now make it possible for clinical laboratories to detect human coronaviruses (hcovs). we investigated the clinical characteristics of hcov-oc43 and hcov-nl63 in patients <5 years of age during a recent coronavirus season. | 2014 | 24577040 |
| multicenter study of viral etiology and relapse in hospitalized children with bronchiolitis. | it is unclear whether the infectious etiology of severe bronchiolitis affects short-term outcomes, such as posthospitalization relapse. we tested the hypothesis that children hospitalized with rhinovirus (rv) bronchiolitis, either as a sole pathogen or in combination with respiratory syncytial virus (rsv), are at increased risk of relapse. | 2014 | 24577039 |
| enzyme-linked immunospot assay for detection of human respiratory syncytial virus f protein-specific gamma interferon-producing t cells. | respiratory syncytial virus (rsv) causes significant disease in elderly adults, and we have previously reported that individuals 65 years of age and older have reduced rsv f protein-specific gamma interferon (ifn-γ)-producing t cells compared to healthy younger adults. to measure rsv f-specific memory t cell responses in the elderly following infection or vaccination, we optimized and qualified an ifn-γ enzyme-linked immunospot (elispot) assay. since peripheral blood mononuclear cells (pbmc) fro ... | 2014 | 24574540 |
| development of an adenovirus-based respiratory syncytial virus vaccine: preclinical evaluation of efficacy, immunogenicity, and enhanced disease in a cotton rat model. | the lack of a vaccine against respiratory syncytial virus (rsv) is a challenging and serious gap in preventive medicine. herein, we characterize the immunogenicity of an adenovirus serotype 5-based rsv vaccine encoding the fusion (f) protein (ad5.rsv-f) and the protection provided following immunization with ad5.rsv-f and assess its potential for producing enhanced disease in a cotton rat (cr) model. animals were immunized intranasally (i.n.) and/or intramuscularly (i.m.) and subsequently challe ... | 2014 | 24574396 |
| detection of common respiratory viruses in tonsillar tissue of children with obstructive sleep apnea. | early life viral infection is associated with neurogenic inflammation that is present in lymphoid tissues of the upper airway in children with obstructive sleep apnea (osa). we hypothesized that viral genomic material is present in tonsils of children with osa. therefore, we examined tonsils for the presence of respiratory viruses' nucleic acids in children with osa, and in children without osa (undergoing surgery for recurrent throat infections (ri)). | 2015 | 24574078 |
| respiratory syncytial virus-related encephalitis: magnetic resonance imaging findings with diffusion-weighted study: response to a "letter to the editor". | 2014 | 24573325 | |
| vaccination with the rsv fusion protein formulated with a combination adjuvant induces long-lasting protective immunity. | respiratory syncytial virus (rsv) is one of the primary causative agents of upper and lower respiratory tract infections in young children, in particular infants. recently, we reported the protective efficacy of a rsv vaccine formulation consisting of a truncated version of the fusion (f) protein formulated with a toll-like receptor (tlr) agonist and an immunostimulatory peptide in a carrier system (δf/triadj). to evaluate the duration of immunity induced by this vaccine candidate, we carried ou ... | 2014 | 24572813 |
| the epidemiology and aetiology of infections in children admitted with clinical severe pneumonia to a university hospital in rabat, morocco. | scarce and limited epidemiological, clinical and microbiological data are available regarding paediatric respiratory tract infections in the kingdom of morocco, a middle-income country in northwestern africa. the results of hospital-based surveillance aiming at describing the aetiology and epidemiology of respiratory distress among children <5 years of age are presented. | 2014 | 24570343 |
| early-life viral infections and the development of asthma: a target for asthma prevention? | to discuss the recent insights into the relationships between viral respiratory infections and asthma inception in the context of a long-term goal of moving toward prevention strategies for childhood asthma. | 2014 | 24569522 |
| innate immune recognition of respiratory syncytial virus infection. | respiratory syncytial virus (rsv) is the leading cause of respiratory infection in infants and young children. severe clinical manifestation of rsv infection is a bronchiolitis, which is common in infants under six months of age. recently, rsv has been recognized as an important cause of respiratory infection in older populations with cardiovascular morbidity or immunocompromised patients. however, neither a vaccine nor an effective antiviral therapy is currently available. moreover, the interac ... | 2014 | 24568879 |
| single, dual and multiple respiratory virus infections and risk of hospitalization and mortality. | respiratory virus infections cause a significant number of hospitalization and deaths globally. this study investigated the association between single and multiple respiratory virus infections and risk of admission to a general ward, intensive care unit or death in patients aged 0-105 years (mean ± s.d. = 24·4 ± 24·1 years), from north west england, that were tested for respiratory virus infections between january 2007 and june 2012. the majority of infections were in children aged ⩽5 years. dua ... | 2015 | 24568719 |
| mortality associated with seasonal and pandemic influenza and respiratory syncytial virus among children <5 years of age in a high hiv prevalence setting--south africa, 1998-2009. | there are few published data describing the mortality burden associated with influenza and respiratory syncytial virus (rsv) infection in children in low- and middle-income countries and particularly from africa and settings with high prevalence of human immunodeficiency virus (hiv). | 2014 | 24567249 |
| clinical utility of pcr for common viruses in acute respiratory illness. | acute respiratory illness (ari) accounts for a large proportion of all visits to pediatric health facilities. quantitative real-time polymerase chain reaction (qpcr) analyses allow sensitive detection of viral nucleic acids, but it is not clear to what extent specific viruses contribute to disease because many viruses have been detected in asymptomatic children. better understanding of how to interpret viral findings is important to reduce unnecessary use of antibiotics. | 2014 | 24567027 |
| human bocavirus respiratory infection in infants in córdoba, argentina. | it has been suggested that human bocavirus (hbov) is related to acute respiratory infection (ari) in children (prevalence: 0.9% to 33%) although clinical characteristics have not been clearly established yet. | 2014 | 24566786 |
| evaluation of respiratory syncytial virus group a and b genotypes among nosocomial and community-acquired pediatric infections in southern brazil. | respiratory syncytial virus (rsv) is the main cause of lower respiratory tract illness in children worldwide. molecular analyses show two distinct rsv groups (a and b) that comprise different genotypes. this variability contributes to the capacity of rsv to cause yearly outbreaks. these rsv genotypes circulate within the community and within hospital wards. rsv is currently the leading cause of nosocomial respiratory tract infections in pediatric populations. the aim of this study was to evaluat ... | 2014 | 24564922 |
| infection with respiratory syncytial virus influences fasl-mediated apoptosis of pulmonary γδ t cells in a murine model of allergen sensitization. | it has been reported that adoptive transfer of γδ t cells increases the cellular infiltration, especially eosinophils, in the lungs of allergic mice, suggesting that γδ t cells may play a proinflammatory role in allergic airway inflammation. respiratory syncytial virus (rsv) infection can decrease the number of th2-type γδ t cells. however, the underlying mechanisms remain unknown. | 2014 | 24564286 |
| sublingual administration of a helper-dependent adenoviral vector expressing the codon-optimized soluble fusion glycoprotein of human respiratory syncytial virus elicits protective immunity in mice. | sublingual (s.l.) immunization has been described as a convenient and safe way to induce mucosal immune responses in the respiratory and genital tracts. we constructed a helper-dependent adenoviral (hdad) vector expressing a condon-optimized soluble fusion glycoprotein (sfsyn) of respiratory syncytial virus (hdad-sfsyn) and explored the potential of s.l. immunization with hdad-sfsyn to stimulate immune responses in the respiratory mucosa. the rsv specific systemic and mucosal immune responses we ... | 2014 | 24560779 |
| respiratory syncytial virus (rsv) infection in elderly mice results in altered antiviral gene expression and enhanced pathology. | elderly persons are more susceptible to rsv-induced pneumonia than young people, but the molecular mechanism underlying this susceptibility is not well understood. in this study, we used an aged mouse model of rsv-induced pneumonia to examine how aging alters the lung pathology, modulates antiviral gene expressions, and the production of inflammatory cytokines in response to rsv infection. young (2-3 months) and aged (19-21 months) mice were intranasally infected with mucogenic or non-mucogenic ... | 2014 | 24558422 |
| ct-morphological characterization of respiratory syncytial virus (rsv) pneumonia in immune-compromised adults. | characterization and follow-up evaluation of chest ct of rsv pneumonia in immune-compromised adults during a seasonal epidemic. | 2014 | 24557598 |
| respiratory syncytial virus-related encephalitis: magnetic resonance imaging findings with diffusion-weighted study. | 2014 | 24553789 | |
| quantitative and qualitative deficits in neonatal lung-migratory dendritic cells impact the generation of the cd8+ t cell response. | cd103+ and cd11b+ populations of cd11c+mhciihi murine dendritic cells (dcs) have been shown to carry antigens from the lung through the afferent lymphatics to mediastinal lymph nodes (mln). we compared the responses of these two dc populations in neonatal and adult mice following intranasal infection with respiratory syncytial virus. the response in neonates was dominated by functionally-limited cd103+ dcs, while cd11b+ dcs were diminished in both number and function compared to adults. infectin ... | 2014 | 24550729 |
| three viruses of the bovine respiratory disease complex apply different strategies to initiate infection. | bovine respiratory disease complex (brdc) is the major cause of serious respiratory tract infections in calves. the disease is multifactorial, with either stress or reduced immunity allowing several pathogens to emerge. we investigated the susceptibility of bovine airway epithelial cells (baec) to infection by the three major viruses associated with the brdc: bovine respiratory syncytial virus (brsv), bovine herpesvirus type 1 (bhv-1) and bovine parainfluenza virus type 3 (bpiv3). for this purpo ... | 2014 | 24548739 |
| clinical and epidemiologic factors related to subsequent wheezing after virus-induced lower respiratory tract infections in hospitalized pediatric patients younger than 3 years. | most wheezing episodes in infants are caused and exacerbated by virus-induced lower respiratory tract infections. however, there are few reports of epidemiologic and clinical virus-specific research with a focus on virus-induced wheezing. the purpose of the current study was to characterize the clinical presentation of virus-induced wheezing in pediatric patients <3 years of age who were hospitalized with lower respiratory tract infections. of the 412 patients in the study, 216 were followed for ... | 2014 | 24535712 |
| lower number of plasmacytoid dendritic cells in peripheral blood of children with bronchiolitis following respiratory syncytial virus infection. | dendritic cells (dcs) are key mediators of allergic airway inflammation. thus, it is important to understand the relationship between respiratory syncytial virus (rsv) infection and dcs, especially in children with rsv bronchiolitis. | 2014 | 24528606 |
| hospital outbreak of human respiratory syncytial virus (hrsv) illness in immunocompromised hospitalized children during summer. | the human respiratory syncytial virus (hrsv) is a community-acquired virus that mainly causes acute respiratory tract infection in infants and children. hrsv is increasingly recognized as an important nosocomial pathogen causing morbidity in immunocompromised patients. here, we are reporting a hospital outbreak of hrsv during summer in children receiving chemotherapy for haematological malignancies. prompt detection and timely preventive measures could abort the devastating outbreak. | 2015 | 24521518 |
| effects of anti-g and anti-f antibodies on airway function after respiratory syncytial virus infection. | respiratory syncytial virus (rsv) is the leading cause of lower respiratory tract illnesses in infants worldwide. both rsv-g and rsv-f glycoproteins play pathogenic roles during infection with rsv. the objective of this study was to compare the effects of anti-rsv-g and anti-rsv-f monoclonal antibodies (mabs) on airway hyperresponsiveness (ahr) and inflammation after primary or secondary rsv infection in mice. in the primary infection model, mice were infected with rsv at 6 weeks of age. anti-rs ... | 2014 | 24521403 |
| molecular detection and phylogenetic analysis of kenyan human bocavirus isolates. | the commonly expected causative agents associated with flu-like symptoms in kenya are the classical viral pathogens identifiable as influenza virus, adenovirus, parainfluenza virus, enteroviruses, respiratory syncytial virus (rsv) and rhinovirus. however, newer agents have been identified globally that present with illnesses clinically indistinguishable from those caused by the classical pathogens; one of them is human bocavirus. | 2014 | 24518633 |
| mortality of live export cattle on long-haul voyages: pathologic changes and pathogens. | the cause of death in 215 cattle on 20 long-haul live export voyages from australia to the middle east, russia, and china was investigated between 2010 and 2012 using gross, histologic, and/or molecular pathology techniques. a quantitative reverse transcription polymerase chain reaction (qrt-pcr) assay was used to detect nucleic acids from viruses and bacteria known to be associated with respiratory disease in cattle: bovine coronavirus (betacoronavirus 1), bovine herpesvirus 1, bovine viral dia ... | 2014 | 24518275 |
| the relationship between respiratory syncytial virus and asthma. | asthma is a chronic inflammatory disease of the lung that is a leading cause of morbidity and mortality in children worldwide. most infants who experience wheezing episodes also exhibit evidence of an ongoing respiratory viral infection. respiratory syncytial virus (rsv) is the leading cause of lower respiratory tract infection in children and is a common cause of wheezing in infants and young children. in the past several decades, a number of studies have demonstrated a relationship between inf ... | 2015 | 24513802 |
| cell-mediated immune responses to respiratory syncytial virus infection: magnitude, kinetics, and correlates with morbidity and age. | we evaluated the cell-mediated immune (cmi) response to rsv acute infection including the magnitude, kinetics and correlates with morbidity and age. twenty-nine rsv-infected patients with mean ± sd age of 15 ± 14 months were enrolled during their first week of disease. th1, th2, th9, th17 and th22 responses were measured at entry and 2 and 6 weeks later. all subjects were hospitalized for a median (range) of 5 (3-11) days. rsv-specific effector and memory th1 cmi measured by lymphocyte prolifera ... | 2014 | 24513666 |
| respiratory syncytial virus- and human metapneumovirus-associated emergency department and hospital burden in adults. | determine the burden of illness associated with respiratory syncytial virus (rsv) and human metapneumovirus (hmpv) in adults, especially young adults. | 2014 | 24512531 |
| hospitalization for respiratory syncytial virus bronchiolitis and disease severity in twins. | respiratory syncytial virus (rsv) is a common cause of lower respiratory tract disease and hospitalization in infants and young children. infants of multiple births, who are often premature, might be more susceptible to developing a more severe rsv infection than singletons. | 2013 | 24511652 |
| clonotype-specific avidity influences the dynamics and hierarchy of virus-specific regulatory and effector cd4(+) t-cell responses. | a key component of immunity against viruses, cd4(+) t cells expand and differentiate into functional subsets upon primary infection, where effector (teff) cells facilitate infection control and regulatory (treg) cells mitigate immunopathology. after secondary infection, teff cells mount a robust response from the memory pool. here, we show that treg-cell responses are diminished upon secondary infection, and treg-cell response dynamics are associated more with t-cell receptors (tcrs) repertoire ... | 2014 | 24510524 |
| children with celiac disease are more likely to have attended hospital for prior respiratory syncytial virus infection. | the purpose of this study was to examine the association between celiac disease (cd) and prior respiratory syncytial virus (rsv) infection or any viral bronchiolitis. | 2014 | 24510390 |
| respiratory syncytial virus persistence in macrophages upregulates fcgamma receptors expression. | viruses can persist in differentiated cells (i.e., macrophages) over long periods of time, altering host cells functions but not inducing their death. we had previously reported that, in early passages (14-40) of a murine macrophage-like cell line persistently infected with respiratory syncytial virus (rsv) (mfp), fcgr-mediated phagocytosis and expression of fcgriib/riii on the cell membrane were increased with respect to mock-infected macrophages (mfn). in this work, we explored the mechanism u ... | 2014 | 24509813 |
| influenza virus vaccine expressing fusion and attachment protein epitopes of respiratory syncytial virus induces protective antibodies in balb/c mice. | respiratory syncytial virus (rsv) is an important viral pathogen that causes life-threatening respiratory infections in both infants and the elderly; no vaccines are at present available. in this report, we examined the use of influenza virus as a vehicle for production of an experimental rsv vaccine. we used reverse genetics to generate a recombinant influenza a virus with epitopes from the rsv fusion (f) and attachment (g) proteins (rflu/rsv/f+g) in the influenza virus nonstructural (ns1) prot ... | 2014 | 24509239 |
| proof of principle for epitope-focused vaccine design. | vaccines prevent infectious disease largely by inducing protective neutralizing antibodies against vulnerable epitopes. several major pathogens have resisted traditional vaccine development, although vulnerable epitopes targeted by neutralizing antibodies have been identified for several such cases. hence, new vaccine design methods to induce epitope-specific neutralizing antibodies are needed. here we show, with a neutralization epitope from respiratory syncytial virus, that computational prote ... | 2014 | 24499818 |
| respiratory viruses within homeless shelters in marseille, france. | homeless shelters are identified as places where humans are at high risk of acquiring respiratory disease. we previously reported the prevalence of the main respiratory diseases affecting a population of homeless in marseille, france. here, we investigated the prevalence of 10 respiratory viruses in a similar homeless population during 2 successive winter seasons. | 2014 | 24499605 |
| blood collected on filter paper for wildlife serology: evaluating storage and temperature challenges of field collections. | filter-paper (fp) blood sampling can facilitate wildlife research and expand disease surveillance. previous work indicated that nobuto fp samples from caribou and reindeer (rangifer tarandus subspecies) had comparable sensitivity and specificity to serum samples (≥ 80% for both) in competitive enzyme-linked immunosorbent assays (celisas) for brucella spp., neospora caninum, and west nile virus. the same sensitivity and specificity criteria were met in indirect elisas for brucella spp., bovine he ... | 2014 | 24499329 |
| clinical epidemiology of bocavirus, rhinovirus, two polyomaviruses and four coronaviruses in hiv-infected and hiv-uninfected south african children. | advances in molecular diagnostics have implicated newly-discovered respiratory viruses in the pathogenesis of pneumonia. we aimed to determine the prevalence and clinical characteristics of human bocavirus (hbov), human rhinovirus (hrv), polyomavirus-wu (wupyv) and -ki (kipyv) and human coronaviruses (cov)-oc43, -nl63, -hku1 and -229e among children hospitalized with lower respiratory tract infections (lrti). | 2014 | 24498274 |
| increased risk of wheeze and decreased lung function after respiratory syncytial virus infection. | a relationship between hospitalization for respiratory syncytial virus (rsv) bronchiolitis and asthma development has been suggested in case-control studies. | 2014 | 24498037 |