Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| nucleotide sequence of the porcine transmissible gastroenteritis coronavirus matrix protein gene. | cdna clones mapping within the first 2601 bases of the 3' end of the tgev genome were sequenced completely or in part by the method of maxam and gilbert and open reading frames were examined. one reading frame yielding a protein having properties of the matrix (m) protein was identified. it is positioned at the immediate 5' side of the nucleocapsid (n) gene but is separated by an intergenic region of 12 bases. the deduced m protein is comprised of 262 amino acids, has a molecular weight of 29,54 ... | 1987 | 2829520 |
| a haemagglutination inhibition test for avian infectious bronchitis virus antibody. | 1987 | 2825628 | |
| enzyme-linked immunosorbent assay for the detection of infectious bronchitis virus antigens. | 1987 | 2824904 | |
| studies of avian urolithiasis associated with an infectious bronchitis virus. | avian urolithiasis syndrome was diagnosed in 14-to-25-week-old chickens from a multiple-age caged-layer complex housing more than 2.5 million chickens. losses from this syndrome ranged from 0.5 to 1.0% per week. seven-to-14-week-old pullets from this facility had multifocal renal tubular necrosis leading to interstitial fibrosis, tophus formation, and tubular dilation. a coronavirus was isolated in embryos inoculated with pooled samples of trachea, kidney, and cecal tonsil of 4-week-old pullets. ... | 1987 | 2823778 |
| serum antibody responses of chickens following sequential inoculations with different infectious bronchitis virus serotypes. | sequential inoculations of chickens with different live infectious bronchitis virus (ibv) antigenic types had major effects on virus-neutralization (vn) and hemagglutination-inhibition (hi) serum antibody responses. antibody production in ibv-inoculated chickens that were reinoculated 8 weeks later with heterologous virus was largely directed against the virus used for initial inoculation rather than the virus used for reinoculation. in addition, chickens inoculated sequentially with ibv produce ... | 1987 | 2823771 |
| comparison of serological tests for antibodies against newcastle disease virus and infectious bronchitis virus using immunocomb solid-phase immunoassay, a commercial enzyme-linked immunosorbent assay, and the hemagglutination-inhibition assay. | combscores determined using the immunocomb solid-phase immunoassay were compared with hemagglutination-inhibition (hi) titers specific for newcastle disease virus (ndv) and infectious bronchitis virus (ibv) and with mean enzyme-linked immunosorbent assay (elisa) titers determined using agritech systems, inc., elisa. combscores for ndv and ibv increased proportionately in a stepwise manner as hi titers increased. the immunocomb solid-phase immunoassay was ablt to produce endpoint titers on sera w ... | 1987 | 2823769 |
| determination of the antigenic relationships among six serotypes of infectious bronchitis virus using the enzyme-linked immunosorbent assay and serum-neutralization test. | the indirect enzyme-linked immunosorbent assay (elisa) was applied to detect antibody to infectious bronchitis (ib) virus in chickens. a cross-reactivity study was performed to determine the antigenic relationships among six strains: massachusetts, connecticut, beaudette, and three ontario field isolates. for comparison, the cross-reactivity study was performed in eggs using the serum-neutralization (sn) test for ib. the elisa proved to be more broadly cross-reactive than the ib-sn test. this su ... | 1987 | 2823768 |
| comparative nephropathogenicity of different strains of infectious bronchitis virus in chickens. | two-week-old white leghorn chickens were injected intravenously with australian t, holte, gray, and m 41 strains of infectious bronchitis virus. a total of 100, 90, 85, and 20% of the chickens infected with australian t, holte, gray, and m 41, respectively, had variable degrees of nephritis. the australian t strain induced the most rapid and the most severe kidney lesions followed by holte, gray, and m 41 in descending order. | 1987 | 2821527 |
| expression of the infectious bronchitis virus spike protein by recombinant vaccinia virus and induction of neutralizing antibodies in vaccinated mice. | a cdna clone of the infectious bronchitis virus (ibv) spike protein gene has been recombined into vaccinia virus. cells infected with the recombinant virus synthesized ibv spike antigen which was recognized by antibody raised against purified spike protein. immunofluorescence showed that the ibv spike antigen was transported to the infected cell surface membrane and immunoprecipitation showed the presence of the glycosylated 180k mol. wt. polypeptide precursor of the two spike subunits s1 and s2 ... | 1987 | 2821170 |
| a specific transmembrane domain of a coronavirus e1 glycoprotein is required for its retention in the golgi region. | the e1 glycoprotein of the avian coronavirus infectious bronchitis virus contains a short, glycosylated amino-terminal domain, three membrane-spanning domains, and a long carboxy-terminal cytoplasmic domain. we show that e1 expressed from cdna is targeted to the golgi region, as it is in infected cells. e1 proteins with precise deletions of the first and second or the second and third membrane-spanning domains were glycosylated, thus suggesting that either the first or third transmembrane domain ... | 1987 | 2821010 |
| re-excretion of an enterotropic infectious bronchitis virus by hens at point of lay after experimental infection at day old. | 1987 | 2820108 | |
| physiological evaluation of diuresis in commercial broiler breeders. | a physiological investigation on an outbreak of diuresis syndrome in commercial broiler breeder hens was carried out. daily water consumption increased 4-fold and daily manure wet weight increased two-fold in affected hens. 2. the syndrome did not have a genetic basis. it was associated with kidney dysfunction which, once acquired, was not alleviated by changing the diet, the drinking water, or the environment. diuresis ceased when water intake was restricted and returned when water was again ma ... | 1989 | 2765979 |
| virulence and transmissibility of mycoplasma gallisepticum. | the virulence of 4 low passage strains of mycoplasma gallisepticum obtained from different sources within australia was studied by experimental infection of chickens. strain ap3as, originally isolated from the air sac of a broiler chicken, produced severe air sac lesions following injection into the abdominal air sacs of 2-week or 3-week-old chickens, and adult hens. strain 80083 which was isolated from a clinically normal broiler breeder hen was also capable of producing gross air sac lesions f ... | 1989 | 2712773 |
| identification and location of the structural glycoproteins of a tissue culture-adapted turkey enteric coronavirus. | the minnesota strain of turkey enteric coronavirus (tcv) was grown on a human rectal tumor (hrt-18) cell line in the presence of radiolabeled amino acids and glucosamine to analyse virion structural proteins. in addition to the 52,000 unglycosylated nucleocapsid protein, three major glycoprotein species were found to be associated with the viral envelope. a predominant glycosylated protein with a molecular weight of 22-24,000 represented the transmembrane matrix protein. larger glycoproteins wit ... | 1989 | 2673155 |
| comparisons of the structural proteins of avian infectious bronchitis virus as determined by western blot analysis. | the antigenic diversity of ten strains of avian infectious bronchitis virus (ibv) was examined by western blot analyses using polyclonal antisera specific for the massachusetts 41 (m41), gray, arkansas dpi (ark dpi), connecticut (conn) and australian t (aust t) serotypes. although antigenic variation was found in all three structural viral proteins, the matrix protein appeared to be antigenically the most highly variable. four distinct antigenic groups, which did not correspond to virulence or p ... | 1989 | 2560918 |
| molecular biology of avian infectious bronchitis virus. | 1989 | 2560396 | |
| an outbreak of nephropathogenic h13 infectious bronchitis in commercial broilers. | a nephropathogenic massachusetts strain of infectious bronchitis virus, designated h13-ib virus, was isolated from the kidneys of broilers in a commercial flock. respiratory distress, diarrhea, depression, and high mortality were present. gross renal lesions consisted of pale coloration, mottling, urate deposition, and swelling. the trachea contained a serous to catarrhal exudate. microscopically, renal changes were primarily associated with the medullary region. lesions included an interstitial ... | 1989 | 2559709 |
| protection afforded infectious bronchitis virus-vaccinated sentinel chickens raised in a commercial environment. | the protective efficacy of three infectious bronchitis virus (ibv) vaccines for sentinel chickens raised with commercial delmarva broiler chickens was evaluated during winter 1987. specific-pathogen-free leghorn sentinel chickens were vaccinated with massachusetts (mass) alone, mass and jmk, or mass and arkansas (ark) combination live vaccines, or they remained unvaccinated. four weeks post-vaccination, sentinels were placed on broiler farms at weekly intervals for 3 weeks corresponding to weeks ... | 1989 | 2559707 |
| pathogenicity of escherichia coli in aerosols for young chickens. | the relative pathogenicity of esherichia coli isolates from poultry was determined by aerosol exposure of young chickens. evidence of colisepticemia with airsacculitis and/or pericarditis and perihepatitis was evaluated. a system was devised that included the intratracheal (it) inoculation of strain se-17 infectious bronchitis virus (ibv) of chicks at 7 days of age followed by their aerosol exposure to e. coli culture suspensions 2 days later. each experiment was terminated 6 days later. for com ... | 1989 | 2559703 |
| comparison of egg-yolk and serum antibody titers to four avian viruses by enzyme-linked immunosorbent assay using paired field samples. | eggs and blood were collected from 11 hens in each of nine broiler-breeder flocks in quebec. serum and egg-yolk extracts were assayed for antibody titers to infectious bursal disease virus (ibdv), infectious bronchitis virus (ibv), newcastle disease virus (ndv), and reovirus (rv) by a commercial enzyme-linked immunosorbent assay (elisa) kit. comparison was made between egg-yolk and serum antibody titers by a regression analysis. a high correlation was observed between serum and yolk antibody tit ... | 1989 | 2559700 |
| practical application of nucleic acid techniques to avian disease problems. | a workshop in which 17 practicing scientists participated was intended to address primarily people who use or could use biotechnology in their work and was confined to five techniques. endonuclease fingerprinting and mapping involved cleaving nucleic acid with a specific restriction enzyme and separating the nucleic acid fragments by electrophoresis. field and vaccine isolates of pasteurella multocida could be distinguished; salmonella enteritidis could be divided into three groups; chlamydia co ... | 1989 | 2559697 |
| avian infectious bronchitis: cross-protection studies using different australian subtypes. | the cross-immunity of vaccinated chickens after challenge with some australian infectious bronchitis viruses was assessed by humoral antibody responses and by ciliary activity in tracheal rings of vaccinated chickens following challenge. four viruses were used for vaccination: vac 3, vac 4, both current infectious bronchitis vaccine viruses, and q1/76 and n2/62. ibv n1/62 (synonym t0 and infectious bronchitis virus n9/74 (synonym appin) were used to challenge the vaccinated chickens. results sho ... | 1989 | 2559688 |
| purification of infectious bronchitis coronavirus by sephacryl s-1000 gel chromatography. | a procedure was developed to purify infectious bronchitis virus (ibv) by gel chromatography (gc) with a sephacryl s-1000 column. virus samples concentrated by centrifugation were applied to a sephacryl s-1000 column and eluted by 0.02 m phosphate buffer (ph 7.2) containing 0.15 m nacl. virus particles were recovered mainly in the first peak. purity of the samples was evaluated by both sodium dodecyl sulfate-polyacrylamide gel electrophoresis and electron microscopy. using electron microscopy, it ... | 1989 | 2558440 |
| a fluorescence test in allantoic cells for the detection of infectious bronchitis virus. | 1989 | 2554867 | |
| order of exposure to high dietary calcium and gray strain infectious bronchitis virus alters renal function and the incidence of urolithiasis. | experiments were designed to determine if gray strain infectious bronchitis virus (ibv) infection increases the incidence of urolithiasis type kidney damage when the urine is already high in ca and relatively alkaline due to a high ca-low available p diet (i.e., layer ration). in addition, experiments were conducted to determine the effects of gray strain ibv on pullet renal function of 6 and 14-wk-old pullets at 2, 5, and 10 days postinoculation (pi). blood gas parameters were measured to deter ... | 1989 | 2554268 |
| nucleotide sequence of the gene coding for the peplomer protein (= spike protein) of infectious bronchitis virus, strain d274. | 1989 | 2550899 | |
| genetic differences in susceptibility to a mixture of avian infectious bronchitis virus and escherichia coli. | two-week-old chickens of 9 inbred and partially inbred lines of chickens were challenged intranasally with a mixed infection consisting of a pool of virulent strains of infectious bronchitis virus and a pool of pathogenic strains of escherichia coli. 2. wide differences in mortality were observed in the different lines, ranging from 3% in a brown leghorn line to 87% in white leghorn line 7(2). 3. experiments involving challenge with e. coli alone or virus alone suggested that this variation refl ... | 1989 | 2545316 |
| detection of turkey enteric coronavirus by enzyme-linked immunosorbent assay and differentiation from other coronaviruses. | a double-antibody elisa for the detection of coronaviruses in intestinal contents from turkey poults with diarrhea was developed. antibodies were raised in rabbits and guinea pigs against a minnesota isolate of turkey enteric coronavirus (tcv) propagated in embryonating turkey eggs and were purified by density-gradient centrifugation. the specificity of antisera was confirmed by hemagglutination-inhibition and immunoelectron microscopy. absorption of anti-tcv hyperimmune sera with egg extracts o ... | 1989 | 2541641 |
| immunohistological identification of both infectious bursal and marek virus antigens in the bursa of fabricius. | indirect immunoperoxidase (iip) and avidin biotin-peroxidase complex (abc) techniques were used for the detection of infectious bursal virus (ibv) and marek disease virus (mdv) antigens in alcohol and formalin-fixed, paraffin-embedded lymphoid tissues from broilers. both techniques appeared potentially useful for the diagnosis of both viral antigens in alcohol-fixed tissues, and allowed the observation of dual infection in the bursa of fabricius of the studied animals in a natural infection. | 1989 | 2540946 |
| antigenic relationship among strains of ibaraki virus and epizootic haemorrhagic disease virus studied with monoclonal antibodies. | twelve monoclonal antibodies against ibaraki virus (ibv) were established and preliminarily characterised by indirect immunoperoxidase (iip), haemagglutination inhibition (hi) and neutralisation (nt) tests. five antibodies reacted in the iip test with all ibv and epizootic haemorrhagic disease virus (ehdv) strains tested, and five antibodies reacted with ibv and alberta strain (serotype 2) but not with new jersey strain (serotype 1) of ehdv. two of 12 antibodies showed both hi and nt activities. ... | 1989 | 2539639 |
| coronavirus genome: prediction of putative functional domains in the non-structural polyprotein by comparative amino acid sequence analysis. | amino acid sequences of 2 giant non-structural polyproteins (f1 and f2) of infectious bronchitis virus (ibv), a member of coronaviridae, were compared, by computer-assisted methods, to sequences of a number of other positive strand rna viral and cellular proteins. by this approach, juxtaposed putative rna-dependent rna polymerase, nucleic acid binding ("finger"-like) and rna helicase domains were identified in f2. together, these domains might constitute the core of the protein complex involved ... | 1989 | 2526320 |
| comparative infectivity for axenic and specific-pathogen-free chickens of o2 escherichia coli strains with or without virulence factors. | adhesion to epithelial respiratory cells, iron acquisition, and production of k1 polysaccharide capsules have been proposed as potential virulence factors of avian escherichia coli. these factors were studied by inoculating groups of axenic or specific-pathogen-free (spf) chickens intratracheally with o2 e. coli strains after previous challenge with a wild strain of infectious bronchitis virus (ibv). in all experiments, the association between ibv and an e. coli strain endowed with the three vir ... | 1989 | 2522766 |
| analysis of an immunodominant region of infectious bronchitis virus. | we analyzed the antigenic fine-structure of an immunodominant region in the peplomer protein of infectious bronchitis virus. this region near the n-terminus of the s2 subunit is recognized by polyclonal antisera and by the majority of mab that cross-react with denatured protein. despite their involvement in neutralization, epitopes in this region were conserved in different serotypes. epitopes of four mab and two chicken antisera were localized by using prokaryotic expression of cdna fragments, ... | 1989 | 2477454 |
| characterization of infectious bronchitis virus using monoclonal antibodies. | three monoclonal antibodies (mabs) reactive against two structural proteins--the nucleoprotein (np) or the surface (s) protein--of avian infectious bronchitis virus (ibv) were produced and characterized. the mabs did not neutralize virus infectivity or inhibit hemagglutination. their reactivity patterns with the homologous strain and eight heterologous strains of ibv were determined using the indirect immunoperoxidase test, the indirect immunofluorescent test, transfer-immunoblotting of separate ... | 1989 | 2476112 |
| antigenicity of the peplomer protein of infectious bronchitis virus. | to study the antigenic structure of the peplomer protein of the avian coronavirus infectious bronchitis virus, fragments from the peplomer gene were generated by restriction-enzyme cleavage or by limited dnase digestion and inserted in the escherichia coli expression plasmid pex (stanley and luzio, 1984). the antigenicity of the expression products was tested using a number of polyclonal antisera and monoclonal antibodies. the polyclonal antisera recognized different sets of epitopes in the 1162 ... | 1989 | 2467199 |
| phylogeny of antigenic variants of avian coronavirus ibv. | the sequences of the peplomeric s1 protein of four serologically distinct strains of the infectious bronchitis virus (ibv), an avian coronavirus, have been determined. the s1 protein is thought to contain the serotype-specific neutralization epitopes and to be the main target of antigenic variation. an alignment with sequences of three strains published previously showed that from the 545 amino acid residues only 243 have been conserved. clustering of substitutions suggests that most serotype de ... | 1989 | 2466369 |
| amino acids within hypervariable region 1 of avian coronavirus ibv (massachusetts serotype) spike glycoprotein are associated with neutralization epitopes. | the spike glycoprotein (s) gene of ibv codes for a precursor protein which is cleaved into the n-terminal s1 and c-terminal s2 glycopolypeptides. the s1 glycopolypeptide, which induces neutralizing antibody, comprises approximately 520 amino acid residues. we have determined the nucleotide sequence of s1 of seven strains of the massachusetts (mass) serotype and the first 337 bases of two additional mass strains. despite the fact that the strains had been isolated over three decades in europe and ... | 1988 | 2462314 |
| interferon induction by several strains of avian infectious bronchitis virus, a coronavirus, in chickens. | eight u.s. and japanese strains of avian infectious bronchitis virus (ibv) were tested for their interferon-inducing ability in chickens. all strains induced interferon (ifn) in several organs of six-week-old spf chickens. however, the extent of ifn formation in these chickens was not necessarily the same from one strain to another. | 1988 | 2454022 |
| comparison of two strains of avian infectious bronchitis virus for their interferon induction, viral growth and development of virus-neutralizing antibody in experimentally-infected chickens. | two field strains of avian infectious bronchitis virus were tested in specific pathogen-free chickens for interferon induction, growth and stimulation of virus-neutralizing antibody. both strains induced interferon in lungs and trachea and neither strain induced it in kidneys. strain kagoshima-34, isolated from the kidneys of a chicken that had died of nephrosis/nephritis, induced interferon earlier than strain tottori-2, which was isolated from the trachea of a chicken with respiratory disease. ... | 1987 | 2449761 |
| properties of coronavirus ibv after removal of the s1 subunit of the spike glycoprotein. | 1987 | 2449046 | |
| the neutralization epitopes on the spike protein of infectious bronchitis virus and their antigenic variation. | 1987 | 2449045 | |
| epitopes on the peplomer protein of infectious bronchitis virus strain m41 as defined by monoclonal antibodies. | sixteen monoclonal antibodies (mcabs) were prepared against infectious bronchitis virus strain m41, all of them reacting with the peplomer protein. one of them, mcab 13, was able to neutralize the virus and to inhibit hemagglutination. competition binding assays allowed the definition of five epitopes, designated as a, b, c, d, and e, of which epitopes a and b are overlapping. furthermore, the binding of mcab 13 (epitope e) could be enhanced by the addition of mcabs from group b, c, and d. a dot ... | 1987 | 2446423 |
| strain specific antibodies revealed by immunoabsorption studies with avian infectious bronchitis virus. | rabbit antisera prepared against the massachusetts 41 (m41) strain of avian infectious bronchitis virus (ibv) and absorbed with chick embryo immunoabsorbent produced multiple precipitin lines in immunodouble-diffusion (idd) tests with homologous or heterologous strains of virus. these precipitin lines were all removed by absorption with concentrated m41 virus preparations, but repeated absorption with concentrated, purified preparations of ibv strains: t, holte, connecticut, beaudette or h120 fa ... | 1987 | 2438843 |
| the peplomer protein sequence of the m41 strain of coronavirus ibv and its comparison with beaudette strains. | the amino acid sequence of the gene for the peplomer protein of the vaccine strain m41 and the beaudette laboratory strain m42-salk of avian infectious bronchitis virus (ibv) have been derived from cdna sequences. as found with other coronaviruses, the peplomer protein carries the epitopes eliciting neutralizing antibodies. the gene encodes a primary translation product of 1162 amino acids with a molecular weight of 128,079. the use of a recent algorithm to predict membrane-protein interactions ... | 1986 | 2429473 |
| [antigenic and pathogenic properties of 3 isolates of infectious bronchitis virus recovered from inoculated birds]. | 1986 | 2422852 | |
| haemagglutination inhibition antibody titres in chickens vaccinated with infectious bronchitis virus vaccines. | 1985 | 2409660 | |
| nucleotide sequence of the gene encoding the spike glycoprotein of human coronavirus hcv 229e. | the gene encoding the spike glycoprotein of the human coronavirus hcv 229e has been cloned and sequenced. this analysis predicts an s polypeptide of 1173 amino acids with an mr of 128,600. the polypeptide has 30 potential n-glycosylation sites. a number of structural features typical of coronavirus s proteins can be recognized, including a signal sequence, a membrane anchor, heptad repeat structures and a carboxy-terminal cysteine cluster. a detailed, computer-aided comparison with the s protein ... | 1990 | 2345367 |
| pathogenesis of h13 nephropathogenic infectious bronchitis virus. | a nephropathogenic massachusetts strain of infectious bronchitis virus (ibv) designated h13-ibv was isolated from the kidneys of commercial broilers. h13-ibv caused respiratory distress, depression, and diarrhea in specific-pathogen-free chickens. gross renal lesions included pale coloration, swelling, and urate deposition. histologic renal changes were interstitial mononuclear cell infiltration and degeneration and necrosis of tubular epithelial cells. lesions in respiratory tissues included th ... | 1990 | 2177977 |
| serological and molecular characterization of three enteric isolates of infectious bronchitis virus of chickens. | three coronaviruses isolated from the intestines of laying chickens were partially characterized. serological and molecular assays indicated that the enteric coronaviruses are infectious bronchitis virus (ibv) isolates. although the three isolates were recovered from three unrelated chicken flocks, their rnase t1-resistant oligonucleotide fingerprints were almost identical. the three isolates were not neutralized by antisera specific to ibv serotype connecticut, but their rnase t1-resistant olig ... | 1990 | 2177976 |
| a monoclonal antibody-based immunoperoxidase procedure for rapid detection of infectious bronchitis virus in infected tissues. | a monoclonal antibody (mab)-based indirect immunoperoxidase (ip) procedure was applied to detect infectious bronchitis virus (ibv) antigen in frozen tissue sections. with this procedure, ibv antigen could be detected in chorioallantoic membranes (cams) within 15 hr postinoculation and in the respiratory tissues of chickens within 96 hr postinoculation. endogenous peroxidases in chicken tissues were removed by treatment with periodic acid. the ip technique was highly sensitive, convenient, and ec ... | 1990 | 2177975 |
| tissue tropism of three cloacal isolates and massachusetts strain of infectious bronchitis virus. | three virus isolates (ecv-1, -2, and -3) recovered from cloacae of chickens in flocks that experienced drops in egg production were identified as infectious bronchitis virus (ibv), based on characteristic embryo lesions, chloroform sensitivity, coronavirus morphology, and serology. because these isolates were recovered from the cloacae of the hens, their tissue tropism was compared with the prototype strain of ibv, massachusetts-41 (m-41), in experimentally inoculated chickens. during the 39-day ... | 1990 | 2177974 |
| early pathogenesis in chicks of infection with an enterotropic strain of infectious bronchitis virus. | one-day-old specific-pathogen-free chicks were inoculated intranasally and intraocularly with infectious bronchitis virus (strain g). at days 1, 3, 5, 7, 10, and 14 postinfection, three birds were euthanatized, and the virus contents of both enteric tissues and some non-enteric tissues were assayed. immunofluorescence and histopathological studies were also conducted. six of 30 chicks died of nephritis between days 5-10 postinfection. gross kidney lesions were the major pathological abnormalitie ... | 1990 | 2177973 |
| a method for the rapid purification of serum igm for the diagnosis of recent viral infections of chickens. | the rapid purification of chicken igm from serum was achieved by affinity chromatography. igm immunoadsorbent gels were prepared using monoclonal antibodies specific to chicken igm. five different eluting agents were compared for the dissociation of the adsorbed igm; the most convenient for routine purposes was 2 m nacl, tris-hcl, edta (nte), as this enabled direct assay of eluents by elisa without requiring the intermediate step of dialysis, which the other eluting agents did. eluents prepared ... | 1990 | 2176659 |
| detection of avian infectious bronchitis viral infection using in situ hybridization and recombinant dna. | a recombinant dna probe with specificity for the 3' end of genomic rna from the ark 99 strain of infectious bronchitis virus (ibv) was found to hybridize with extracted rna of three strains with the ark serotype, as well as the mass41, holl52, gray, jmk, conn, fla and se17 strains of ibv. viral infection was detected in the cytoplasm of chicken embryo kidney cells inoculated with mass41, ark99, se17 or two recent field isolates of ibv using in situ cytohybridization and a biotinylated probe. in ... | 1990 | 2175524 |
| examination of sera from game birds for antibodies against avian viruses. | 1990 | 2171182 | |
| the e1 glycoprotein of an avian coronavirus is targeted to the cis golgi complex. | it was previously reported that the e1 protein of an avian coronavirus was targeted to the juxtanuclear region in cos cells expressing the protein from cloned cdna, suggesting that the protein contains information for targeting to the golgi complex. the first of three membrane-spanning domains was required for intracellular targeting, because a mutant e1 (delta m1,2) lacking this domain was delivered to the plasma membrane. we have used immunoelectron microscopy to localize the wild-type e1 prot ... | 1990 | 2169615 |
| enzyme-linked immunosorbent assay for the detection of infectious bronchitis virus (ibv) antigen with monoclonal antibody. | 1990 | 2166855 | |
| screening for antibodies against infectious bronchitis virus: specificity of the haemagglutination inhibition test. | a screening of antibodies against strain m-41 and the dutch variant strains d-274 and d-1466 of infectious bronchitis virus (ibv) using the haemagglutination inhibition test (hi) was carried out in chile. the presence of these variant strains has not been reported yet and therefore the probability of finding positive flocks is low. on this basis some statistical analysis were made with those data obtained in order to contribute to the problem of the specificity of the hi test for ibv diagnosis. ... | 1990 | 2166411 |
| comparisons of the genomic rna of arkansas dpi embryonic passages 10 and 100, australian t, and massachusetts 41 strains of infectious bronchitis virus. | the genomic ribonucleic acid (rna) of arkansas dpi at embryonic passages 10 and 100, australian t, and massachusetts 41 strains of infectious bronchitis virus (ibv) were compared using two-dimensional, 32ppcp-labeled oligonucleotide fingerprinting. the arkansas dpi strain embryonic passage 10 was pathogenic for chickens and had one oligonucleotide not present in the attenuated passage 100. the remaining arkansas dpi oligonucleotides had identical electrophoretic mobility. the fingerprint pattern ... | 1990 | 2164385 |
| detection of infectious bronchitis virus antigen from experimentally infected chickens by indirect immunofluorescent assay with monoclonal antibody. | infectious bronchitis virus (ibv) was detected by indirect immunofluorescent assay with a monoclonal antibody (mab-ifa). the monoclonal antibody was specific for the nucleocapsid protein of ibv strain m41. the mab-ifa clearly detected ibv with high specificity in infected chicken kidney cells. the assay furthermore detected ibv in tracheal smears and sliced tracheas from experimentally infected chickens. the positive reaction was found to be longer than that in the virus recovery test. these res ... | 1990 | 2164384 |
| a model for determining immunogenic relationships between avian infectious bronchitis viruses. | a model for determining immunogenic relationships between strains of infectious bronchitis virus is described that is based upon the vaccinating dose required to induce prevention of multiplication of a standard challenge dose of an homologous strain in the lungs of specific-pathogen-free chickens. the model has been used to demonstrate that: (1) approximately 100 times the vaccinating dose must be used to produce immunity from one compared with two doses; (2) differences of immunogenicity of gr ... | 1990 | 2162161 |
| nucleotide and amino acid sequence of the s1 subunit of the spike glycoprotein of avian infectious bronchitis virus, strain d3896. | 1990 | 2161519 | |
| detection of antibody to avian viruses in human populations. | the ability of three avian viruses to elicit antibody response in humans was surveyed for the purpose of identifying zoonotic diseases. antibody levels in people associated with poultry were compared to those in people having limited poultry association. antibody levels to three avian viruses: infectious bursal disease virus, a birnavirus; newcastle disease virus, a paramyxovirus; and avian infectious bronchitis virus, a coronavirus were determined by enzyme-linked immunosorbent assays (elisa). ... | 1990 | 2161349 |
| the primary structure and expression of the second open reading frame of the polymerase gene of the coronavirus mhv-a59; a highly conserved polymerase is expressed by an efficient ribosomal frameshifting mechanism. | sequence analysis of a substantial part of the polymerase gene of the murine coronavirus mhv-a59 revealed the 3' end of an open reading frame (orf1a) overlapping with a large orf (orf1b; 2733 amino acids) which covers the 3' half of the polymerase gene. the expression of orf1b occurs by a ribosomal frameshifting mechanism since the orf1a/orf1b overlapping nucleotide sequence is capable of inducing ribosomal frameshifting in vitro as well as in vivo. a stem-loop structure and a pseudoknot are pre ... | 1990 | 2159623 |
| antigenic and genomic relationships among turkey and bovine enteric coronaviruses. | antigenic and genomic relationships among tissue culture-adapted turkey enteric coronavirus (tcv) isolates, three strains of avian infectious bronchitis virus (ibv), and mammalian coronaviruses were investigated. immunoblotting and immunoprecipitation experiments using polyclonal antisera showed that the four major structural proteins of tcv cross-reacted with the four homologous proteins of bovine enteric coronavirus (bcv), the n and m proteins of mouse hepatitis virus serotype 3, and the n pro ... | 1990 | 2159566 |
| immune response to oil-emulsion vaccines with single or mixed antigens of newcastle disease, avian influenza, and infectious bronchitis. | inactivated newcastle disease virus (ndv), avian influenza virus (aiv), and infectious bronchitis virus (ibv) antigens were evaluated for immunological efficacy in monovalent and polyvalent vaccines. vaccinated broilers were bled for hemagglutination-inhibition (hi) tests at 1- or 2-week intervals. half of the chickens were challenged with the largo isolate of velogenic viscerotropic (vv) ndv at 8 weeks post-vaccination, and the remainder were challenged with the massachusetts 41 strain ibv at 9 ... | 1990 | 2157390 |
| identification of a new membrane-associated polypeptide specified by the coronavirus infectious bronchitis virus. | nucleotide sequences from the third open reading frame of mrna d (d3) of infectious bronchitis virus (ibv) were expressed in bacteria as part of a fusion protein with beta-galactosidase. antiserum raised in rabbits against this fusion protein immunoprecipitated from ibv-infected chick kidney or vero cells a polypeptide of 12.4k, the size expected for a d3-encoded product. the d3 polypeptide is apparently non-glycosylated, and appears to be associated with the membrane fraction of infected cells, ... | 1990 | 2154538 |
| safety of temperature sensitive mutant mycoplasma gallisepticum vaccine. | a temperature sensitive (ts) vaccine strain designated ts-11 was selected after exposure of a low passage culture of the immunogenic australian field isolate (strain 80083) of mycoplasma gallisepticum to 100 mg/ml of n-methyl-n-nitro-n-nitrosoguanidine. viable counts (assayed as colour changing units (ccu)/25 microliters) of a thawed stock culture of ts-11 were typically log10 3 to log10 5 higher when incubated at 33 degrees c (the permissive temperature) than duplicate viable counts incubated a ... | 1990 | 2143067 |
| comparison of persistence of seven bovine viruses on bovine embryos following in vitro exposure. | the ability of seven cytopathic strains of bovine viruses to adhere to the zona pellucida of six-to-eight day-old bovine embryos were compared. embryos were exposed to virus by placing them either in virus suspensions or by culturing them on infected bovine turbinate cultures for 18-24 h. after exposure to bovine virus diarrhea virus (bvdv), infectious bovine rhinotracheitis virus (ibv), bluetongue virus (btv), pseudorabies virus (prv), vesicular stomatitis virus (vsv), parainfluenza 3 virus (pi ... | 1990 | 2107070 |
| investigations into resistance of chicken lines to infection with infectious bronchitis virus. | although nine inbred and partially inbred lines of chickens were equally susceptible initially to infection with infectious bronchitis virus (ibv), the outcome of that infection varied considerably between the lines. a more detailed study of a line which was resistant (line c) and one which was highly susceptible (line 15i) confirmed that both lines could be infected initially with ibv but showed that the respiratory tract epithelium of line c chicks recovered more rapidly than that of line 15i ... | 1990 | 1966442 |
| selection of variants of avian infectious bronchitis virus showing tropism for different organs. | avian infectious bronchitis virus strain kagoshima-34 isolated from the kidneys of a chicken that died of nephrosis/nephritis lost its nephropathogenicity during intratracheal passage in spf chickens. the resultant virus acquired stronger respirotropism but reduced tropism for kidneys. on the other hand strain tottori-2 isolated from the trachea of a chicken suffering from severe respiratory disease did not lose its respirotropism after serial intravenous passage in spf chickens. the serological ... | 1990 | 1966427 |
| sequence comparisons of the 3' end of the genomes of five strains of avian infectious bronchitis virus. | 1990 | 1966426 | |
| molecular basis of the variation exhibited by avian infectious bronchitis coronavirus (ibv). | ibv serotype-specific, virus neutralising (vn) antibody is induced by the s1 subunit of the spike (s) glycoprotein. a portuguese isolate (port/322/85) is considered to be an in vivo recombinant. vn tests showed that it belongs to the massachussetts (mass) serotype. correspondingly, the sequence of s1 and s2 was extremely similar to that of older mass isolates. however, the matrix (m) glycoprotein and upstream gene sequences were atypical of these mass strains and more closely resembled other ser ... | 1990 | 1966424 |
| role of ph in syncytium induction and genome uncoating of avian infectious bronchitis coronavirus (ibv). | syncytium formation induced in vero cells by ibv-beaudette was optimal at ph 6.7 and absent at ph 7.5. reduction of ibv-beaudette replication by ammonium chloride, which raises the ph in endosomes, further indicated that uncoating of the virus genome probably occurred within the acidic environment of endosomes. however, this would not appear to apply to all ibv strains, since one was unaffected by the drug. it would appear that some strains of ibv have the capacity to fuse with the plasma membra ... | 1990 | 1966419 |
| nucleotide sequence of the e2-peplomer protein gene and partial nucleotide sequence of the upstream polymerase gene of transmissible gas gastroenteritis virus (miller strain). | the e2-peplomer protein gene of the virulent miller strain of transmissible gastroenteritis virus (tgev) was sequenced from cdna clones and compared to the e2 gene sequence of the avirulent purdue strain. sequence comparisons indicate that most amino acid differences occur in the n-terminal half of the e2-peplomer which represents the most exposed region of the protein. in addition, analysis of an incompletely sequenced open reading frame (orf) to the immediate 5' side of the e2 gene indicates e ... | 1990 | 1966416 |
| products of the polymerase-encoding region of the coronavirus ibv. | 1990 | 1966413 | |
| a ribosomal frameshift signal in the polymerase-encoding region of the ibv genome. | 1990 | 1966412 | |
| the effects of three reproductive hormones and cortisone on the replication of avian infectious bronchitis virus in vitro. | the in-vitro effects of three reproductive hormones (progesterone, oestrogen testosterone), and cortisone on the replication of infectious bronchitis (ib) virus strain g were investigated over a period of 36 hours using tracheal organ cultures as the culture system. the non-toxic concentration of each hormone for the culture system was first determined. these were found to be 3 micrograms/ml for progesterone, testosterone, and cortisone, and 1 micrograms/ml for oestrogen. the results were based ... | 1990 | 1966009 |
| isolation and characterization of new infectious bronchitis virus variants in hungary. | two agents not agglutinating chicken erythrocytes were isolated, one in each of two flocks, from organ samples and tracheal swabs taken from 4- to 7-week-old chicks of 8 broiler flocks experiencing respiratory signs. virus isolation was done in embryonated spf hen's eggs. morphological changes of the embryos, appearing as dwarfing or curling into a spherical form, usually occurred in the 3rd or 4th passage on postinoculation (pi) days 5-9. some embryos had swollen kidneys covered with urate. ele ... | 1990 | 1965995 |
| sequence of the s gene from a virulent british field isolate of transmissible gastroenteritis virus. | subgenomic mrna from a virulent field isolate of porcine transmissible gastroenteritis virus (tgev), strain fs772/70, was used to produce cdna. the cdna from three overlapping clones was sequenced by the chain termination method and two open reading frames (orfs) were identified. the largest orf, 4350bp, encoded a polypeptide of 1449 amino acids of relative molecular mass (mr) 159811, which contained 33 potential n-linked glycosylation sites, a cysteine-rich region, and a potential transmembrane ... | 1990 | 1964522 |
| association of the infectious bronchitis virus 3c protein with the virion envelope. | a highly purified radiolabeled preparation of the coronavirus infectious bronchitis virus (ibv) was analyzed, by immunoprecipitation with monospecific antisera, for the presence of a series of small virus proteins recently identified as the products of ibv mrnas 3 and 5. one of these, 3c, a 12.4k protein encoded by the third open reading frame of the tricistronic mrna3 was clearly detectable and was found to cofractionate with virion envelope proteins on detergent disruption of virus particles. ... | 1991 | 1962461 |
| health survey of backyard poultry and other avian species located within one mile of commercial california meat-turkey flocks. | a survey was conducted to characterize domestic and exotic bird populations, estimate seroprevalence to selected disease agents, and describe health management practices on 62 premises containing "backyard" flocks located within one mile of 22 commercial california meat-turkey flocks participating in national animal health monitoring system (nahms). chickens were present on 56 backyard premises and turkeys on seven. antibodies were identified against mycoplasma gallisepticum, m. synoviae, m. mel ... | 1991 | 1854324 |
| variant serotypes of infectious bronchitis virus isolated from commercial layer and broiler chickens. | twenty infectious bronchitis virus (ibv) field isolates obtained from commercial layer and broiler chickens in 1987 and 1988 were serotyped using the virus-neutralization (vn) test. six different previously unrecognized variant serotypes were identified from a total of seven isolates from layer chickens. only two isolates, both from maine, were the same variant serotype. variant serotypes also were recovered from layer flocks in illinois and washington and the province of ontario, canada. two di ... | 1991 | 1851422 |
| polymerase chain reaction amplification of the genome of infectious bronchitis virus. | the polymerase chain reaction (pcr) was used to amplify a portion of the genome of infectious bronchitis virus (ibv). two synthetic primers that annealed to segments of the ibv genome in the matrix and nucleocapsid genes facilitated pcr amplification of a 1020-base sequence. amplification was successful using template dna from an ibv sequence-containing plasmid and using copy dna created by reverse transcription of ibv genomic rna. the pcr product was the expected size and had the expected nucle ... | 1991 | 1851417 |
| efficacy of coarse-spray administration of a reovirus vaccine in young chickens. | coarse-spray (cs) administration of a commercial s1133 reovirus vaccine in chickens for prevention of clinical viral tenosynovitis (vt) infection was evaluated. in expt. 1, one-day-old specific-pathogen-free (spf) white leghorns were vaccinated with a combination of reovirus, newcastle disease (nd), and infectious bronchitis (ib) vaccines by cs and infectious bursal disease vaccine by the subcutaneous (sq) route. in expt. 2, one-day-old commercial broilers were vaccinated by cs with reovirus vac ... | 1991 | 1851416 |
| mhc class ii-restricted t-cell hybridomas recognizing the nucleocapsid protein of avian coronavirus ibv. | mice were immunized with purified infectious bronchitis virus (ibv), strain m41. spleen cells, expanded in vitro by stimulation with m41, were immortalized by fusion to obtain t-cell hybridomas, and two major histocompatability complex (mhc) class ii (i-e)-restricted t-cell hybridomas were selected with specificity for ibv. both hybridomas selectively recognized the internal nucleocapsid protein. the responses to 12 different strains of ibv varied markedly. this demonstrates antigenic variation ... | 1991 | 1847691 |
| the complete sequence (22 kilobases) of murine coronavirus gene 1 encoding the putative proteases and rna polymerase. | the 5'-most gene, gene 1, of the genome of murine coronavirus, mouse hepatitis virus (mhv), is presumed to encode the viral rna-dependent rna polymerase. we have determined the complete sequence of this gene of the jhm strain by cdna cloning and sequencing. the total length of this gene is 21,798 nucleotides long, which includes two overlapping, large open reading frames. the first open reading frame, orf 1a, is 4488 amino acids long. the second open reading frame, orf 1b, overlaps orf 1a for 75 ... | 1991 | 1846489 |
| monoclonal antibodies to three structural proteins of avian infectious bronchitis virus: characterization of epitopes and antigenic differentiation of australian strains. | ten monoclonal antibodies (mabs) directed against three structural proteins of infectious bronchitis viruses (ibv), the peplomer (s), membrane (m) and nucleocapsid (n) proteins, were characterized and used to determine the antigenic relationship between australian ibv strains. one mab (mab 5) was directed against an epitope on the s1 subunit of the peplomer, another (mab 2) against an epitope on the m glycoprotein and eight mabs (mabs 1, 7, 9, 16, 24, 26, 27 and 51) were directed against seven n ... | 1991 | 1722501 |
| localization of a t-cell epitope within the nucleocapsid protein of avian coronavirus. | in a previous study, two murine t-cell hybridomas generated after immunization with infectious bronchitis virus (ibv) were shown to be responsive to the internally localized viral nucleocapsid protein. in the present study, the antigenic determinants were mapped using recombinant expression products and synthetic peptides. both hybridomas recognized the region spanning amino acid residues 71 to 78 of the nucleocapsid protein. the experimentally determined epitope corresponded with predicted moti ... | 1991 | 1718856 |
| the nucleocapsid protein of ibv comprises immunodominant determinants recognized by t-cells. | 1990 | 1715661 | |
| binding of antibodies that strongly neutralise infectious bronchitis virus is dependent on the glycosylation of the viral peplomer protein. | 1990 | 1715656 | |
| sequence evidence for rna recombination in field isolates of avian coronavirus infectious bronchitis virus. | under laboratory conditions coronaviruses were shown to have a high frequency of recombination. in the netherlands, vaccination against infectious bronchitis virus (ibv) is performed with vaccines that contain several life-attenuated virus strains. these highly effective vaccines may create ideal conditions for recombination, and could therefore be dangerous in the long term. this paper addresses the question of the frequency of recombination of avian coronavirus ibv in the field. a method was s ... | 1990 | 1708184 |
| antigenic domains on the peplomer protein of avian infectious bronchitis virus: correlation with biological functions. | monoclonal antibodies (mabs) directed against structural proteins of infectious bronchitis virus (ibv) were produced to analyse the antigenic structure of this virus. competitive binding of enzyme-labelled and unlabelled mabs to ibv peplomer protein was analysed in an antibody binding assay to test the relatedness of the epitopes defined by the mabs. based on the competition groups, eight epitope clusters were defined (s-a to s-h); six of these clusters (s1-a to s1-f) were located on the s1 subu ... | 1990 | 1698920 |
| typing of recent infectious bronchitis virus isolates causing nephritis in chicken. | 1991 | 1681795 | |
| a new typing method for the avian infectious bronchitis virus using polymerase chain reaction and restriction enzyme fragment length polymorphism. | two primers with the length of 22 bases each and 400 bases apart on the spike protein gene of avian infectious bronchitis virus (ibv) were prepared. using these primers, the genome rna from twelve strains of the various serotypes were reverse-transcribed to cdna and amplified by polymerase chain reaction (pcr). with all strains, 400 base dna was amplified, indicating that there were no apparent insertions or deletions in this region. however, the amplified dna showed different cleavage patterns ... | 1991 | 1672064 |
| pathobiology of cryptosporidiosis (c. baileyi) in broiler chickens. | pathologic and clinicopathologic changes were examined in broiler chickens inoculated with cryptosporidium baileyi (cb) alone or in combination with infectious bronchitis virus (ibv), infectious bursal disease virus (ibdv) or escherichia coli (ec). concurrent infections with cb and either ibv or ec resulted in a greater respiratory inflammatory response than either agent given alone. concurrent cb, ibv or ec infections resulted in a decreased density of respiratory cryptosporidial stages. no int ... | 1991 | 1667932 |
| the effects of oestrogen and progesterone on re-excretion of infectious bronchitis virus strain g (correction of straing) in spf chickens. | the effects of oestrogen and progesterone on the re-excretion of ib virus strain g in chickens infected at day-old was evaluated in this study. following infection of the chickens at day-old, the birds were swabbed regularly from the trachea and cloaca until no more virus was isolated from either site. between 10 and 14 weeks of age oestrogen and progesterone were administered by intramuscular injection to infected or control chickens. an infected but non-hormone injected group was maintained. a ... | 1991 | 1666637 |
| serological response in broiler chicks to different commercial newcastle disease and infectious bronchitis vaccines. | broiler chicks were administered vaccines against newcastle disease and infectious bronchitis (both arkansas and massachusetts strains) at 2 weeks of age as either primary or secondary vaccinations. the vaccine was administered as a spray at 2 weeks of age to chicks that had received newcastle disease vaccine alone, bronchitis vaccine alone, both vaccines in combination, or no vaccine at day 1 in the hatchery. the newcastle disease hemagglutination-inhibition response was significantly lower in ... | 1991 | 1664724 |
| attenuation of avian infectious bronchitis virus by cold-adaptation. | avian infectious bronchitis virus (ibv) arkansas-type dpi strain was passaged 10 times in specific-pathogen-free (spf) chicken embryos incubated at 28 c and 37 c. virus grown at 28 c acquired cold-adapted (ca) and temperature-sensitive (ts) characteristics based on more-rapid growth at 28 c and a reduced ability to grown at 41 c, respectively, compared with non-cold-adapted (non-ca) virus grown at 37 c. the pathogenicity and immunogenicity were determined for ca and non-ca ibv in 1-day-old spf c ... | 1991 | 1664720 |
| effects of trypsin and sodium tauroglycocholate on an enterotropic variant of ib virus. | 1991 | 1664553 |