Publications
Title | Abstract | Year Filter | PMID(sorted descending) Filter |
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quantitative recovery of listeria monocytogenes and select salmonella serotypes from environmental sample media. | environmental sampling has become increasingly important in the food industry for monitoring the presence of specific pathogens such as listeria monocytogenes and salmonella enterica. several microbiological media are available for storage and transport of environmental samples from the processing plant to the test laboratory. in this study, we quantified the survival of l. monocytogenes, s. typhimurium, s. enteritidis, and s. typhi in environmental sampling media over several time and temperatu ... | 2007 | 17373457 |
fatal salmonella enteritidis septicaemia in a rheumatoid arthritis patient treated with a tnf-alpha antagonist. | we report a patient with a rare presentation of extra-intestinal salmonellosis after infliximab therapy for rheumatoid arthritis. we discuss the increasing incidence of primary infections and reactivation of intracellular microorganisms after treatment with tnf-alpha blockage, with emphasis on salmonellosis. | 2007 | 17366020 |
case reports of transient loss of vision and systemic lupus erythematosus. | neuropsychiatric manifestations can occur in up to two-thirds of patients with systemic lupus erythematosus (sle). the presentations as well as the underlying immunopathogenic mechanisms can be heterogeneous and therefore have an enormous impact on therapeutic options. | 2007 | 17364083 |
the chicken host peptides, gallinacins 4, 7, and 9 have antimicrobial activity against salmonella serovars. | the gallinacin genes clustered on chromosome 3 of the chicken (gallus gallus domesticus) genome encode a group of cationic antimicrobial peptides characteristic of the beta-defensins. in this study, gallinacins 4, 7, and 9, all predicted to contain the conserved pattern of cysteines typical of beta-defensins but differing in their charge and hydrophobicity, were characterised for their in vivo gene expression patterns and in vitro antimicrobial activities against salmonella serovars. reverse-tra ... | 2007 | 17346671 |
[lipopolysaccharides and lipoteichoic acids stimulate rat mast cells to cysteinyl leukotriene synthesis]. | we have investigated the ability of lipopolysaccharides (lps) and lipoteichoic acids (lta) to induce rat peritoneal mast cells to degranulation and histamine release, and to cysteinyl leukotriene (lt) generation. we have stated that lps salmonella enteritidis, lps escherichia coli o111:b4 and lps e. coli o55:b5 did not activate rat mast cells to degranulation and histamine release. however, lpss induced lt synthesis and secretion; the strongest stimulant to generation of lt was lps e. coli o55:b ... | 2006 | 17340997 |
effects of heat-degraded sugars on survival and growth of vibrio parahaemolyticus and other bacteria. | we studied the effects of autoclaved (121 degrees c, 15 min) sugar solutions on the survival and growth of vibrio parahaemolyticus and other bacteria. the growth and survival of v. parahaemolyticus in luria-bertani media and phosphate buffer, respectively, were inhibited by the addition of d-glucose autoclaved in ph 8.0 phosphate buffer. the bactericidal effect of autoclaved d-glucose was very small when autoclaved in ph 7.0 phosphate buffer, but larger effects were observed when autoclaved in t ... | 2007 | 17340871 |
multiple typing for the epidemiological study of the contamination of broilers with salmonella from the hatchery to the slaughterhouse. | eighteen belgian broiler flocks were followed from the hatchery to the slaughterhouse by a multiple typing approach (sero-, geno-, and phage types) for the investigation of the transmission of salmonella and its subtypes. for 12 of the 18 flocks, there was no correlation between the serotypes found preharvest and those isolated from the feces in the transport crates and on the carcasses in the slaughterhouse. serotypes found in the crates were usually also found on the carcasses. in 5 of the 10 ... | 2007 | 17340865 |
infantile meningitis due to salmonella enteritidis. | 2007 | 17337838 | |
milk fermented by lactobacillus helveticus r389 and its non-bacterial fraction confer enhanced protection against salmonella enteritidis serovar typhimurium infection in mice. | bacterial infections in the gastrointestinal tract represent a major global health problem, even in the presence of normally effective mucosal immune mechanisms. milk fermented by lactobacillus helveticus r389 (fm) or its non-bacterial fraction obtained by milk fermentation at controlled ph 6 (nbf) are able to activate the small intestine mucosal immune response according to previous studies. in this work we aimed at comparing their protection capacity against an infection by salmonella enteriti ... | 2007 | 17336831 |
[presternal abscess due to salmonella enterica serovar enteritidis]. | 2007 | 17335709 | |
glicentin inhibits internalization of enteric bacteria by cultured int-407 enterocytes. | glicentin, the main component of enteroglucagon, has trophic effects on intestinal mucosa. it may also have an inhibitory effect on extraintestinal invasion of enteric bacteria. we have established an in vitro bioassay system for determining the effects of recombinant human glicentin on bacterial internalization by confluent enterocytes. an int-407 cell line was serum-deprived for 2 days and was then treated on transwell filters for 24 h with a medium containing one of the following: glicentin 1 ... | 2007 | 17333210 |
kinetics of the human antibody response against salmonella enterica serovars enteritidis and typhimurium determined by lipopolysaccharide enzyme-linked immunosorbent assay. | two indirect enzyme-linked immunosorbent assays (elisas) were employed to measure levels of immunoglobulin g (igg), igm, and iga antibodies against salmonella in sera from 303 danish patients diagnosed by fecal culture with either salmonella enterica serovar enteritidis or salmonella enterica serovar typhimurium infections. the elisas were based on serovar enteritidis lipopolysaccharide (lps) and serovar typhimurium lps. the antibody levels were assessed approximately 1, 3, 6, and 12 months afte ... | 2007 | 17329442 |
salmonella enteritidis in broiler chickens, united states, 2000-2005. | us department of agriculture food safety and inspection service (fsis) data on salmonella enterica serotype enteritidis in broiler chicken carcass rinses collected from 2000 through 2005 showed the annual number of isolates increased >4-fold and the proportion of establishments with salmonella enteritidis-positive rinses increased nearly 3-fold (test for trend, p<0.0001). the number of states with salmonella enteritidis in broiler rinses increased from 14 to 24. the predominant phage types (pt) ... | 2006 | 17326935 |
food poisoning outbreak caused by salmonella enteritidis in box lunches distributed by a restaurant. | 2007 | 17314437 | |
an outbreak of nalidixic acid-resistant salmonella enterica serovar enteritidis at a nursery school in kitakyushu city, japan. | 2007 | 17314431 | |
molecular and biochemical characterizations of human oral lactobacilli as putative probiotic candidates. | the objective of this study was to characterize the lactobacilli from the human oral cavity as a potential source of probiotic strains. | 2007 | 17311634 |
prevalence of qnr genes in salmonella in france. | to detect the qnra, qnrb and qnrs genes among salmonella isolates received at the french national reference centre for salmonella in paris, france. | 2007 | 17307773 |
relationship of pulsed-field profiles with key phage types of salmonella enterica serotype enteritidis in europe: results of an international multi-centre study. | salmonella is one of the most common causes of foodborne infection in europe with salmonella enterica serovar enteritidis (s. enteritidis) being the most commonly identified serovar. the predominant phage type for s. enteritidis is phage type (pt) 4, although pt 8 has increased in incidence. within these phage types, pulsed-field gel electrophoresis (pfge) provides a method of further subdivision. the international project, salm-gene, was established in 2001 to develop a database of pfge profile ... | 2007 | 17306053 |
immunomodulation by mercuric chloride in vitro: application of different cell activation pathways. | evidence is emerging that exposure to mercury (hg) may elicit many pathological manifestations, including immunomodulation. we tested whether changing cellular activation pathways may affect the immunomodulation by hg. human cell cultures were set up where isolated peripheral blood mononuclear cells, activated by monoclonal antibodies (moab: anti-cd3/-cd28/-cd40) or heat-killed salmonella enterica serovar enteritidis (hk-se), exposed to mercuric chloride (hgcl2) for 24 h. cell vitality was asses ... | 2007 | 17302730 |
intragastric immunization with recombinant lactobacillus casei expressing flagellar antigen confers antibody-independent protective immunity against salmonella enterica serovar enteritidis. | a recombinant lactobacillus casei expressing a flagellar antigen from salmonella enterica serovar enteritidis was constructed and evaluated as a mucosal vaccine. intragastric immunization of the recombinant strain conferred protective immunity against salmonella infection in mice. this immunization did not result in antigen-specific antibody in either feces or sera but induced the release of ifn-gamma on restimulation of primed lymphocytes ex vivo. the results suggested that the protective effic ... | 2007 | 17287050 |
induction of human beta-defensin 2 by the probiotic escherichia coli nissle 1917 is mediated through flagellin. | human beta-defensin 2 (hbd-2) is an inducible antimicrobial peptide synthesized by the epithelium to counteract bacterial adherence and invasion. proinflammatory cytokines, as well as certain bacterial strains, have been identified as potent endogenous inducers. recently, we have found that hbd-2 induction by probiotic escherichia coli nissle 1917 was mediated through nf-kappab- and ap-1-dependent pathways. the aim of the present study was to identify the responsible bacterial factor. e. coli ni ... | 2007 | 17283097 |
effects of probiotics and maternal vaccination on salmonella enteritidis infection in broiler chicks. | the effects of probiotics and maternal vaccination with an inactivated salmonella enteritidis (se) vaccine on day-old chicks challenged with se were evaluated. a 2 x 3 factorial arrangement was used (with or without probiotics; breeders nonvaccinated, vaccinated intramuscularly, or vaccinated intraperitoneally). three trials were conducted in isolation cabinets and se challenge was different between trials. the number of se organisms per chick and the time interval between housing and introducti ... | 2006 | 17274302 |
lessons learned from a salmonella enteritidis phage type 4 outbreak in austria, 2005. | an outbreak of gastroenteritis due to salmonella enteritidis phage type 4 occurred in people who attended a traditional hunting festivity in a small village in western austria 6 through 11 november 2005. of approximately 250 attendees, 227 had consumed dishes offered at the festival, and of these consumers 35 persons fulfilled the outbreak case definition (attack rate of 15.4%). spätzle (traditional pastalike side dish) was most likely the contaminated part of the incriminated main course (relat ... | 2007 | 17265857 |
a live salmonella enterica serovar enteritidis vaccine allows serological differentiation between vaccinated and infected animals. | three precisely defined deletion mutants of salmonella enterica serovar enteritidis were constructed, a guanine auxotrophic deltaguab mutant, a nonflagellated deltaflic mutant, and an auxotrophic and nonflagellated deltaguab deltaflic double mutant. all three mutants were less invasive than the wild-type strain in primary chicken cecal epithelial cells and the human epithelial cell line t84 and less efficiently internalized in the chicken macrophage cell line hd11. the deltaflic mutant was patho ... | 2007 | 17261603 |
[foodborne infections and intoxications in poland in 2004]. | a total of 19 870 bacterial foodborne infections and intoxications were registered in 2004 (incidence 52.0/100,000 population). these illnesses were less incident in 2004, compared both to 2003 figure (20 221 cases) and the median 1998-2002 (26 734 cases). unlike bacterial infections, the incidence of viral foodborne infections increased in the recent years (2004 - 27.2 vs. 2003 -21.1 and median 1998-2002 - 10.0). the parasitic foodborne infections did not change much during the recent years. a ... | 2006 | 17249166 |
[salmonellosis in poland in 2004]. | in 2004, 15958 salmonellosis cases were reported (16617 in previous year), the incidence rate was 41,8 per 100 000 population. seventy-one percent of patients were hospitalized, however the hospitalization ratio was higher for extraintestinal manifestations - 89%. as in previous years the seasonal peak was observed between june and august. the most frequently isolated type remained salmonella enteritidis--above 87% of cases. only four other serotypes (typhimurium, hadar, virchow and infantis) we ... | 2006 | 17249164 |
comparison of map and tyrosine kinase signaling in heterophils from commercial and wild-type turkeys. | protein tyrosine and mitogen-activated kinases are crucial mediators of the host innate immune response, conferring signals from surface receptors on the host cell to the nucleus of the cell where gene expression occurs. heterophils were isolated from wild-type rio grande turkeys and a commercial line of turkeys (line a) on days 4 and 7 post-hatch. heterophils were stimulated for 1h with salmonella enteritidis (se) or opsonized se (opse). after stimulation, cells were lysed and lysates were test ... | 2007 | 17241661 |
[decrease in bactericidal activity of ciprofloxacin in salmonella mutants generated following repeated exposure to various fluorquinolones]. | salmonella mutants with reduced fluoroquinolone susceptibility were generated in vitro following repeated exposure to subinhibitory concentrations of the drugs and the alterations in the gyra gene were characterized. afterwards, the change in bactericidal activity exhibited by ciprofloxacin against the resulting mutants was determined. a decrease in the bactericidal activity of ciprofloxacin was found in all the mutants, but was more pronounced in mutants of nalidixic acid-resistant strains. thi ... | 2006 | 17235406 |
case report: parotid abscess due to salmonella enterica serovar enteritidis in an immunocompetent adult. | there are reports of increasing incidence of focal extra-intestinal infections from non-typhoidal salmonellae during the past two decades. we present the first case of a parotid abscess caused by salmonella enterica serovar enteritidis (s. enteritidis) in an apparently immunocompetent adult without other abnormality of the parotid glands. a 58-year-old man was admitted to our hospital because of a 3-day history of fever and painful swelling of the right parotid gland. his medical history was unr ... | 2007 | 17234452 |
evaluation of a modified single-enzyme amplified fragment length polymorphism (se-aflp) technique for subtyping salmonella enterica serotype enteritidis. | salmonella enterica subsp. enterica serotype enteritidis is not readily subtyped beyond the level of phage type (pt). pulsed field gel electrophoresis (pfge) is generally acknowledged to be the most discriminating typing method for salmonella, but only a restricted variety of pfge types has been described for s. enterica serotype enteritidis. in the present study, a modification of the se-aflp typing method was used to investigate both outbreak and apparently sporadic isolates of s. enterica ser ... | 2007 | 17224257 |
salmonella contamination in non-uk produced shell eggs on retail sale in some regions of england. | 2006 | 17213558 | |
an outbreak of salmonella enteritidis infections linked to a restaurant in riga, august 2006. | 2006 | 17213541 | |
non-enteric salmonella spontaneous peritonitis--a new complication of an old immunosuppressant? | 2007 | 17206683 | |
[postoperative meningitis caused by an unusual etiological agent: salmonella enteritidis]. | salmonella intracranial infections are unusual in adults, and particularly after neurosurgical procedures. we report a case of post-operative meningitis owing to salmonella enteritidis. a 72-year-old man with type 2 diabetes was admitted to our unit for postoperative evaluation and management after neoplasic intracranial surgery. on the third day of admission, the patient developed infectious signs and diagnosis of postoperative meningitis was evoked. the cultivation of cerebrospinal fluid revea ... | 2006 | 17203905 |
plasmid profiles and randomly amplified polymorphic dna analysis of salmonella enterica serotype enteritidis strains from outbreaks and sporadic cases in turkey. | the aim of the study was to investigate the characteristics of salmonella serotype enteritidis strains isolated from outbreaks and sporadic cases in turkey by plasmid profiles and randomly amplified polymorphic dna (rapd) patterns. a total of 64 s. enteritidis clinical strains were selected from the culture collection of the enterobacteria laboratory of ankara university medical school department of microbiology and clinical microbiology for molecular analysis using the plasmid profiles and rapd ... | 2006 | 17201091 |
overview and summary of the food safety and inspection service risk assessment for salmonella enteritidis in shell eggs, october 2005. | in 1998, the united states department of agriculture's food safety and inspection service (fsis) and the food and drug administration completed a risk assessment that indicated multiple interventions along the farm-to-table chain were needed to reduce the risk of human illness from salmonella enteritidis in shell eggs. based on newly available data and improved modeling techniques, fsis completed an updated risk assessment to examine the effect of pasteurization and refrigeration on reducing hum ... | 2006 | 17199522 |
rhabdomyolysis and acute renal failure in a child with salmonella enteritidis gastroenteritis. | we report on an 11-year-old boy who developed rhabdomyolysis and acute renal failure following salmonella enteritidis gastroenteritis. rhabdomyolysis should be considered as a potentially fatal complication in patients with salmonella gastroenteritis. | 2007 | 17195937 |
[time course changes in cytokines content and gas composition in blood of patients with salmonellosis and acute shigellosis]. | to study changes in serum concentrations of interleukines (il) and tumor necrosis factor alpha (tnfa) in the course of acute moderate and severe shigellosis and salmonellosis; to elicit their correlation with disorders of gas and electrolyte blood composition and acid-base balance (abb). | 2006 | 17195521 |
[pseudomembranous colitis due to salmonella infection]. | 2006 | 17194344 | |
searching for bacterial lysozyme inhibitors. | 2006 | 17191480 | |
use of linear, weibull, and log-logistic functions to model pressure inactivation of seven foodborne pathogens in milk. | survival curves of six foodborne pathogens suspended in ultra high-temperature (uht) whole milk and exposed to high hydrostatic pressure at 21.5 degrees c were obtained. vibrio parahaemolyticus was treated at 300 mpa and other pathogens, listeria monocytogenes, escherichia coli o157:h7, salmonella enterica serovar enteritidis, salmonella enterica serovar typhimurium, and staphylococcus aureus were treated at 600 mpa. all the survival curves showed a rapid initial drop in bacterial counts followe ... | 2007 | 17188197 |
food poisoning outbreak of salmonella enteritidis caused by box lunch in shiga prefecture, japan. | 2006 | 17186966 | |
influence of commercial sanitizers on lipopolysaccharide production by salmonella enteritidis atcc 13076. | the effect of typical sanitizers on the composition and toxicity of lipopolysaccharides (lpss) produced by salmonella enteritidis atcc 13076 was analyzed. salmonella enteritidis was propagated up to the late exponential phase in the presence of commercial sanitizing solutions. lps was extracted and derivatized with trifluoroacetylation, and gas chromatography-mass spectrometry analysis and the chromogenic limulus amoebocyte lysate assay were used to assess the ultrastructure and toxicity of the ... | 2006 | 17186655 |
response of two salmonella enterica strains inoculated in model cheese treated with high hydrostatic pressure. | the aim of this work was to determine the response to high hydrostatic pressure and the ability for survival, recovery, and growth of 2 strains of salmonella enterica (salmonella enteritidis and salmonella typhimurium) inoculated in a washed-curd model cheese produced with and without starter culture. inoculated samples were treated at 300 and 400 mpa for 10 min at room temperature and analyzed after treatment and after 1, 7, and 15 d of storage at 12 degrees c to study the behavior of the salmo ... | 2007 | 17183079 |
characterisation of community acquired non-typhoidal salmonella from bacteraemia and diarrhoeal infections in children admitted to hospital in nairobi, kenya. | in sub-saharan africa community-acquired non-typhoidal salmonella (nts) is a major cause of high morbidity and death among children under 5 years of age especially from resource poor settings. the emergence of multidrug resistance is a major challenge in treatment of life threatening invasive nts infections in these settings. | 2006 | 17173674 |
the disaccharide trehalose inhibits proinflammatory phenotype activation in macrophages and prevents mortality in experimental septic shock. | proinflammatory phenotype activation in macrophages (mphis) after sepsis orchestrates an inflammatory response leading to multiple organ dysfunction. trehalose preserves cell viability during exposure to a range of environmental stresses. we investigated whether trehalose may inhibit endotoxin-induced activation of the inflammatory phenotype in mphis. rat peritoneal mphis were stimulated with 50 microg/ml of salmonella enteritidis lipopolysaccharide (lps). stimulated mphis were coincubated with ... | 2007 | 17172986 |
[occurence of salmonellae in laying hens in different housing systems and inferences for control]. | as eggs represent now as ever the most important source for salmonella infection in human beings and because of the currently occurring shift in housing conditions for laying hens from conventional cages to alternative systems it was studied whether the salmonella prevalence in layers is influenced by the housing system. following systems were considered: organic farming with free range management systems, floor management systems with free range, floor management systems without free range, con ... | 2006 | 17172134 |
immunological and gene expression responses to a salmonella infection in the chicken intestine. | besides infection in humans, salmonella enteritidis can also cause serious illness in young chickens. however, the genetic and immunological parameters important for the disease in chickens are not well characterized. in this study, processes in the chicken intestine in response to a salmonella infection were investigated in two different chicken lines. one-day-old chickens were orally infected with salmonella. t-cell subpopulations, phagocytic properties of intestinal mononuclear cells and rna ... | 2007 | 17156737 |
parotid abscess due to salmonella enteritidis: a case report. | salmonella infection of the parotid gland is rare. | 2006 | 17152282 |
infective parotitis. | 2006 | 17152268 | |
comparison of multiple-locus variable-number tandem repeat analysis, pulsed-field gel electrophoresis, and phage typing for subtype analysis of salmonella enterica serotype enteritidis. | strain subtyping is an important tool for detection of outbreaks caused by salmonella enterica serotype enteritidis. current subtyping methods, however, yield less than optimal subtype discrimination. in this study, we describe the development and evaluation of a multiple-locus variable-number tandem repeat analysis (mlva) method for subtyping salmonella serotype enteritidis. the discrimination ability and epidemiological concordance of mlva were compared with those of pulsed-field gel electroph ... | 2007 | 17151203 |
necrotizing fasciitis caused by salmonella enteritidis. | a 57-year-old immunocompromized female developed a necrotizing fasciitis with sepsis a few days after abdominal complaints and diarrhoea. surgery was performed because of progressive worsening of the patient's situation and during surgery the decision was made to perform an amputation. after surgery the patient was brought to the intensive care department for a few days. she recovered from her sepsis within a few days. cultures showed salmonella enteritidis. | 2006 | 17148088 |
comparison of the reveal test, the u.s. food and drug administration culture method, and selective media for recovery of salmonella enteritidis from commercial egg layer flock environments. | salmonella is the leading cause of foodborne illnesses in the united states, and salmonella enteritidis (se) is the second most frequently isolated salmonella serovar. egg products are most often associated with outbreaks of se infection. to prevent se contamination of eggs, many producers are implementing flock inspections for se at their facilities. a rapid and simple method for detecting se in poultry environmental samples is critical for effective control of se. in this study, the reveal tes ... | 2006 | 17133825 |
heat tolerance of salmonella enterica serovars agona, enteritidis, and typhimurium in peanut butter. | recent large foodborne outbreaks caused by salmonella enterica serovars have been associated with consumption of foods with high fat content and reduced water activity, even though their ingredients usually undergo pasteurization. the present study was focused on the heat tolerance of salmonella enterica serovars agona, enteritidis, and typhimurium in peanut butter. the salmonella serovars in the peanut butter were resistant to heat, and even at a temperature as high as 90 degrees c only 3.2-log ... | 2006 | 17133812 |
survival, elongation, and elevated tolerance of salmonella enterica serovar enteritidis at reduced water activity. | growing microorganisms on dry surfaces, which results in exposure to low water activity (a(w)), may change their normal morphology and physiological activity. in this study, the morphological changes and cell viability of salmonella enterica serovar enteritidis challenged to low a(w) were analyzed. the results indicated that exposure to reduced a(w) induced filamentation of the cells. the amount of filamentous cells at a(w) 0.94 was up to 90% of the total number of cells. surviving filamentous c ... | 2006 | 17133811 |
in silico model as a tool for interpretation of intestinal infection studies. | in nutrition research the number of human in vivo experiments is limited because of the many restrictions and the high costs of testing in humans. up to now predictive computer models aiming to enhance research have been rare or too complex, with many nonmeasurable adjustable parameters. this study aimed to develop a basic physicochemical computer model for a first quantitative interpretation of results obtained from in vivo intestinal experiments with bacteria. this new modeling approach is val ... | 2007 | 17122404 |
infection and excretion of salmonella enteritidis in two different chicken lines with concurrent ascaridia galli infection. | studies on the impact of interaction of salmonella enterica serovar enteritidis and the parasitic nematode ascaridia galli with the avian host were undertaken with particular emphasis on infection and excretion of these pathogens in two different layer lines. a total of 148 salmonella-free 1-day-old chickens (73 hellevad and 75 lohmann brown) were randomly divided into five groups for each line. group 1 served as an uninoculated control group. groups 2 and 3 were infected with a. galli and s. en ... | 2006 | 17121738 |
the feathering gene is linked to degranulation and oxidative burst not cytokine/chemokine mrna expression levels or salmonella enteritidis organ invasion in broilers. | in the past, we showed differences in heterophil function between parental broilers (a [fast feathering] > b [slow feathering]) and their f1 reciprocal crosses (d [fast feathering] > c [slow feathering]). in the present study, we evaluated the linkage of the feathering gene to heterophil function, pro-inflammatory cytokine/chemokine mrna expression levels, and resistance to salmonella enteritidis organ invasion. heterophils were isolated from 2-day-old chickens (c and d) separated into males and ... | 2006 | 17121735 |
a new monoterpene glycoside and antibacterial monoterpene glycosides from paeonia suffruticosa. | antibacterial activity-guided fractionation of the chci3-meoh (1:1) extract of paeonia suffruticosa root bark furnished three monoterpene glycosides, 6-o-vanillyoxypaeoniflorin (1), mudanpioside-h (2), and galloyl-oxypaeoniflorin (3). of the isolated compounds, compound 1 is a new compound. all isolated compounds showed broad, but moderate, antibacterial activity with minimum inhibitory concentration (mic) values in the range of 100 to 500 microg/ml against eighteen pathogenic microorganisms of ... | 2006 | 17121173 |
synthesis and biological study of medicinally important mannich bases derived from 4-(dimethylamino)-1,4,4a,5,5a,6,11,12a-octahydro-3,6,10,12,12a pentahydroxy naphthacene carboxamide. | the paper describes synthesis and antibacterial study of biologically active mannich bases of carboxamide derivative employing mannich reaction of 4-(dimethylamino)-1,4,4a,5,5a,6,11,12a-octahydro-3,6,10,12,12a pentahydroxy naphthacene carboxamide with various sulfonamides/secondary amines . they were analysed by elemental analysis and characterized by uv, ir and (1)h nmr spectroscopic studies. the mannich bases were screened for antibacterial activity against various gram-negative bacteria at va ... | 2007 | 17110110 |
isolation of salmonella enteritidis phage type 21b from a eurasian eagle-owl (bubo bubo). | a case of fatal salmonellosis in a eurasian eagle-owl (bubo bubo) from bursa province (northwestern turkey) is described. the organs of the bird were examined histopathologically and microbiologically. macroscopic and microscopic findings were consistent with a salmonella infection. salmonella enterica subspecies enterica serovar enteritidis (s. enteritidis) phage type (pt) 21b was isolated from the liver and spleen in pure culture and from the intestine. the isolate was susceptible to amoxycill ... | 2006 | 17092905 |
bacteraemia among severely malnourished children infected and uninfected with the human immunodeficiency virus-1 in kampala, uganda. | to establish the magnitude of bacteraemia in severely malnourished children, and describe the types of bacteria and antimicrobial sensitivity by hiv status. | 2006 | 17090299 |
joint spatial analysis of gastrointestinal infectious diseases. | a major obstacle in the spatial analysis of infectious disease surveillance data is the problem of under-reporting. this article investigates the possibility of inferring reporting rates through joint statistical modelling of several infectious diseases with different aetiologies. once variation in under-reporting can be estimated, geographic risk patterns for infections associated with specific food vehicles may be discerned. we adopt the shared component model, proposed by knorr-held and best ... | 2006 | 17089949 |
a novel multiplex pcr assay for the detection of salmonella enterica serovar enteritidis in human faeces. | to develop a multiplex pcr assay for the detection of salmonella enterica serovar enteritidis in human faeces. | 2006 | 17083715 |
salmonella produces an o-antigen capsule regulated by agfd and important for environmental persistence. | in this study, we show that salmonella produces an o-antigen capsule coregulated with the fimbria- and cellulose-associated extracellular matrix. structural analysis of purified salmonella extracellular polysaccharides yielded predominantly a repeating oligosaccharide unit similar to that of salmonella enterica serovar enteritidis lipopolysaccharide o antigen with some modifications. putative carbohydrate transport and regulatory operons important for capsule assembly and translocation, designat ... | 2006 | 17079680 |
home or away? investigation of salmonella enteritidis pfge pattern senxai.0003 and senbni.0003, phage type 8, the in the maritimes, 2005. | 2006 | 17076033 | |
comparison of the adhesion ability of different salmonella enteritidis serotypes to materials used in kitchens. | contamination of kitchen surfaces due to bacteria present in foodstuffs is one of the main causes of foodborne outbreaks. salmonella infections are an important cause of foodborne disease, and salmonella enteritidis is the most common isolate in the past few years. in this study, the adhesion ability of four salmonella enteritidis isolates to different materials (polyethylene, polypropylene, and granite) used in kitchens was compared. the results indicated that the two plastic materials were gen ... | 2006 | 17066912 |
evaluation of the reveal quick test for salmonella detection in raw chicken meat. | salmonella belongs to the enterobacteriaceae family, and is the cause of illnesses such as enteric fevers, enteritis and septicemia. accordingly, a necessity exists of researching and developing new techniques,--which must be sensible, specific, economic and fast--for genus determination; all this related to the culture technique (official technique). in the present work, 80 samples of chicken were analyzed for salmonella both by the reveal equipment and by the culture technique with and without ... | 2001 | 17061491 |
the yfgl lipoprotein is essential for type iii secretion system expression and virulence of salmonella enterica serovar enteritidis. | salmonella enterica, like many gram-negative pathogens, uses type three secretion systems (ttss) to infect its hosts. the three ttss of salmonella, namely, ttss-1, ttss-2, and flagella, play a major role in the virulence of this bacterium, allowing it to cross the intestinal barrier and to disseminate systemically. previous data from our laboratory have demonstrated the involvement of the chromosomal region harboring the yfgl, enga, and yfgj open reading frames in s. enterica serovar enteritidis ... | 2007 | 17060472 |
involvement of phosphatidylinositol-phospholipase c in immune response to salmonella lipopolysacharide in chicken macrophage cells (hd11). | the activation of phospholipases is one of the earliest key events in receptor-mediated cellular responses to a number of extracellular signaling molecules. lipopolysaccharide (lps) is a principle component of the outer membrane of gram-negative bacteria and a prime target for recognition by the innate immune system. in the present study, we evaluated the role of specific phospholipase in the activation of a chicken macrophage cell line hd11 by lps. activation of hd11 cells by lps results in ind ... | 2006 | 17052668 |
eggshell penetration of hen's eggs by salmonella enterica serovar enteritidis upon various storage conditions. | 1. the survival and penetration of salmonella enterica serovar enteritidis (se) inoculated on the eggshell was examined upon storage for up to 20 d at real-life conditions (15 to 25 degrees c and 45 to 75% relative humidity (rh)). 2. penetration was assessed by emptying the egg contents and filling the eggs with a selective medium that allowed visualising salmonella growth on the inside of the shell and membrane complex. 3. the study of survival on the eggshells was based on viable counts and sh ... | 2006 | 17050098 |
characterization of a nonfimbrial mannose-sensitive hemagglutinin (msh) produced by salmonella enterica serovar enteritidis. | a nonfimbrial mannose-sensitive hemagglutinin (msh) with adhesive properties produced by salmonella enterica serovar enteritidis was characterized. the msh was characterized as glycoprotein and consisted of three noncovalently bound subunits of m(r) 28, 33 and 40 kda determined by sds-page. the hemagglutinin was heat-stable and resistant to alkaline (high) or acid (low) ph, however, it was inhibited by proteolytic enzymes, by edta and by sodium periodate. mouse antiserum raised against msh react ... | 2006 | 17049985 |
inhibition of salmonella-induced il-8 synthesis and expression of hsp70 in enterocyte-like caco-2 cells after exposure to non-starter lactobacilli. | oral administration of lactobacilli as probiotics is gaining importance in the treatment of intestinal inflammations. we investigated the effect of non-starter lactobacilli lactobacillus casei subsp casei 2756, lactobacillus curvatus 2775, and lactobacillus plantarum 2142 as well as their spent culture supernatants (scs) on salmonella enteritidis 857 growth, interleukin (il)-8 and heat shock protein 70 (hsp70) synthesis in undifferentiated crypt-like and differentiated villus-like caco-2 cells. ... | 2006 | 17045688 |
[liver abscess by salmonella within a metastases of müllerian uterin cancer]. | 2006 | 17043011 | |
comparison of salmonella enterica serovar enteritidis levels in crops of fed or fasted infected hens. | long-term feed withdrawal has been shown to increase ileocecal intestinal colonization and fecal shedding of salmonella enterica serovar enteritidis in challenged hens. less information is available regarding effects of fasting on crop colonization. two trials were conducted to compare effects of 14-day feed withdrawal vs. full feed on crop colonization in hens challenged with salmonella enteritidis. the levels of salmonella enteritidis in the crops of fasted hens were significantly higher than ... | 2006 | 17039844 |
effects of nonimmunized egg yolk powder-supplemented feed on salmonella enteritidis prevention and elimination in broilers. | chicken consumption is a newly identified risk factor in salmonella enterica serovar enteritidis (se) infection in humans. se is widely distributed in commercial chicken flocks and high levels of cecal carriage and shedding may lead to broiler meat contamination. in the present study, the preventive and eliminative effect of nonimmunized freeze-dried egg yolk powder (eyp) on se in broilers was investigated. in the prevention trial, reduced se counts were observed in liver (p < or = 0.05), cecal ... | 2006 | 17039835 |
cytokine expression in chicken peripheral blood mononuclear cells after in vitro exposure to salmonella enterica serovar enteritidis. | cytokines are secreted proteins involved with cell recruitment and regulation of both innate and adaptive immune responses. they are essential for an effective host immune response to pathogens. the objective of this study was to determine the effect of salmonella enterica serovar enteritidis (s. enteritidis) exposure and genetic line on cytokine mrna expression level of cultured chicken peripheral blood mononuclear cells (pbmc). interleukin-2, interleukin-6 (il-6), cxcli2, and transforming grow ... | 2006 | 17032822 |
assessment of factors contributing to changes in the incidence of antimicrobial drug resistance in salmonella enterica serotypes enteritidis and typhimurium from humans in england and wales in 2000, 2002 and 2004. | an investigation into changes in the occurrence of antimicrobial resistance in salmonella enterica serotypes enteritidis and typhimurium from human infection in england and wales in 2000, 2002 and 2004 has shown that the incidence of strains of s. enteritidis with resistance to nalidixic acid coupled with decreased susceptibility to ciprofloxacin has more than doubled between 2000 and 2004, whereas the overall levels of resistance in s. typhimurium have fallen by ca. 25%. in relation to publishe ... | 2006 | 17029756 |
improved diagnostic and real-time pcr in rapid screening for salmonella in the poultry food chain. | the goal of this study was to improve the diagnostic applicability of genus- and serovar- (s. enteritidis and s. typhimurium) specific pcr systems in screening faecal and caecal samples of poultry, poultry feed and poultrymeat for salmonella, by keeping the opportunity to obtain salmonella cultures from positive samples. peptone broth pre-enrichment cultures of the samples were tested by pcr. in faecal and caecal samples from broiler chicks a strong inhibitory action was frequently observed. thi ... | 2006 | 17020134 |
[erythema nodosum in a child with salmonellosis]. | we report a case of 4-years-old girl who suffered one episode of clinically typical erythema nodosum and arthralgia of short duration. stool culture showed growth of salmonella enteritidis. levels of serum anti-salmonella antibodies and immunocomplexes lowered during resolution of skin nodules. erythema nodosum probably results from the formation of immunocomplexes and their deposition in and around blood vessels of the skin. salmonellosis should be considered in patients presenting with erythem ... | 2006 | 17017494 |
energy partitioning and thyroid hormone levels during salmonella enteritidis infections in pullets with high or low residual feed intake. | this experiment was conducted to investigate whether feed efficiency, as measured by residual feed intake as a phenotypic trait, affects energy partitioning in pullets that have received salmonella inoculation as an immune challenge. in each of 8 trials, energy partitioning was measured during 5 wk in 15-wk-old efficient (r-) and nonefficient (r+) pullets, which were housed per efficiency group in 2 identical climate respiration chambers. after 1 wk of adaptation, the pullets in 4 trials were or ... | 2006 | 17012168 |
screening for anti-infective properties of several medicinal plants of the mauritians flora. | several plants of the mauritian flora alleged to possess anti-infective properties were studied against different strains of pathogenic bacteria and fungi. the grounded dried plant materials were extracted with different extractants and screened for anti-microbial activity using the disk diffusion and the micro-dilution techniques. preliminary screening revealed that the methanol extracts were most active. salmonella enteritidis, enterobacter cloacae and bacillus subtilis were the three test org ... | 2007 | 17011733 |
interference of salmonella enteritidis and lactobacillus spp. with il-8 levels and transepithelial electrical resistance of enterocyte-like caco-2 cells. | caco-2 cells (exhibiting characteristics of mature villus enterocytes) were used to determine bacteria (salmonella enteritidis causing human gastroenteritis)-intestinal cell interactions. the interference of bacteria with the transepithelial electrical resistance (teer) of filter-grown caco-2 cells and the production of il-8 after exposure of the cells to s. enteritidis 857 and/or lactobacillus strains (l. gasseri lf221 and l. rhamnosus bgt10) was evaluated. the strain 857 decreased teer of filt ... | 2006 | 17007422 |
illegal use of nitrofurans in food animals: contribution to human salmonellosis? | recent observations in portugal of a remarkable incidence (65%) of salmonella isolates from several sources (predominantly human and poultry) with decreased susceptibility to nitrofurantoin (mic > or =64 mg/l), mostly comprising serogroup d isolates of salmonella enteritidis belonging to different phage types, suggest that illegal use of nitrofurans, especially in the poultry industry, might have contributed to the selection and prevalence of s. enteritidis in food animals, and consequently to h ... | 2006 | 17002603 |
activity of brassica oleracea leaf juice on foodborne pathogenic bacteria. | many vegetables of the cruciferae family have been found to possess antimicrobial properties against several microorganisms of clinical importance. in this study, we reported the antibacterial effect of brassica oleracea juice on several food-borne pathogens. the juice was found to be effective in inhibiting the growth of salmonella enteritidis, verotoxigenic escherichia coli o157:h7, e. coli hb producing thermolabile toxin, nontoxigenic e. coli, and listeria monocytogenes, but not enterococcus ... | 2006 | 16995537 |
inhibitory effects of enterococcus strains obtained from a probiotic product on in vitro growth of salmonella enterica serovar enteritidis strain ifo3313. | enterococcus faecium and enterococcus gallinarum strains were isolated from a commercial probiotic product and the effects of these strains on the growth of salmonella enterica serovar enteritidis strain ifo3313 were investigated. viable cell counts of salmonella enteritidis in mixed cultures with the probiotic product isolate of e. faecium were significantly (p < 0.05) lower than those in pure cultures after 6, 8, and 24 h when the cultures were incubated in heart infusion broth at 37 and 41 de ... | 2006 | 16995534 |
inactivation of salmonella serovars in liquid whole egg by heat following irradiation treatments. | salmonella is a frequent contaminant on eggs and is responsible for foodborne illnesses in humans. ionizing radiation and thermal processing can be used to inactivate salmonella in liquid whole egg, but when restricted to doses that do not affect egg quality, these technologies are only partially effective in reducing salmonella populations. in this study, the effect of ionizing radiation in combination with thermal treatment on the survival of salmonella serovars was investigated. of the six sa ... | 2006 | 16995507 |
thermal injury and recovery of salmonella enterica serovar enteritidis in ground chicken with temperature, ph, and sodium chloride as controlling factors. | cells of salmonella enterica serovar enteritidis were grown at 25 and 35 degrees c, heat injured (55, 60, and 62.5 degrees c), and recovered in tryptic soy broth (tsb) at various nacl concentrations (2.0 and 3.5%) and ph levels (5.5 and 6.5). to assess the interactions of growth temperature, heating temperature, nacl concentration and ph on the thermal injury and recovery of salmonella enteritidis in ground chicken, a randomized design with each experimental combination was used. when a logistic ... | 2006 | 16995506 |
nadph oxidases in the gastrointestinal tract: a potential role of nox1 in innate immune response and carcinogenesis. | the gastrointestinal epithelium functions as physical and innate immune barriers against commensal or pathogenic microbes. nadph oxidase 1 (nox1) and dual oxidase 2 (duox2), highly expressed in the colon, are suggested to play a potential role in host defense. guinea-pig gastric pit cells and human colonic epithelial cells (t84 cells) express nox1. with regard to activation of nox1, the gastric epithelial cells are primed with helicobacter pylori lipopolysaccharide, whereas t84 cells preferentia ... | 2006 | 16987012 |
novel method for estimating viable salmonella cell counts using real-time pcr. | a novel method for estimating viable salmonella enteritidis cell counts with 5'-nuclease real-time pcr was developed in this study. our method was based on the increase kinetics of the target dna region (inva) of the microorganism growing in a food/clinical sample in a culture medium during incubation. the index of increase in the target dna region studied here was threshold cycle, ct. a test salmonella strain was grown in buffered peptone water at the optimal temperature (39 degrees c). as salm ... | 2006 | 16984034 |
cxcl16 regulates cell-mediated immunity to salmonella enterica serovar enteritidis via promotion of gamma interferon production. | cxcl16 is a recently discovered multifaceted chemokine that has been shown not only to recruit activated t lymphocytes but also to play a direct role in the binding and phagocytosis of bacteria by professional antigen-presenting cells. in this study, we investigated the role of cxcl16 in vivo in the regulation of the immune response using a murine model of salmonella enterica serovar enteritidis infection. the expression of cxcl16 was strongly upregulated in the spleens and livers of animals dev ... | 2006 | 16982830 |
gene expression responses to a salmonella infection in the chicken intestine differ between lines. | poultry products are an important source of salmonella enterica. an effective way to reduce food poisoning due to salmonella would be to breed chickens more resistant to salmonella. unfortunately host responses to salmonella are complex with many factors involved. to learn more about responses to salmonella in young chickens, a cdna microarray analysis was performed to compare gene expression profiles between two chicken lines under control and salmonella infected conditions. newly hatched chick ... | 2006 | 16978708 |
the presence of major world-wide clones for phage type 4 and 8 salmonella enterica serovar enteritidis and the evaluation of their virulence levels by invasiveness assays in vitro and in vivo. | seventy-seven animal isolates of salmonella enterica serovar enteritidis (s. enteritidis) obtained from the united states were analyzed by phage typing and pulsed field gel electrophoresis (pfge). thirty-nine strains were found with phage types (pt) 4, 8, and 13a. when the chromosomal dna of these 39 isolated strains with pt4, 8, and 13a were digested with xbai, spei and noti, followed by pfge analysis, 28 strains were found with a pattern combination of x4s4n4, which was the major subtype. when ... | 2006 | 16978349 |
in vitro study of salmonella enteritidis and salmonella typhimurium definitive type 104: survival in egg albumen and penetration through the vitelline membrane. | salmonella enteritidis and salmonella typhimurium definitive type 104 (dt104) have been detected in the chicken oviduct, and their survival in egg albumen at the chicken body temperature of 42 degrees c may be important in oviductal and transovarian contamination of intact shell eggs. eight s. enteritidis and 24 s. typhimurium dt104 strains were tested for their in vitro survival in egg albumen. the concentration of the organisms declined more rapidly when incubated at 42 degrees c than at 37 de ... | 2006 | 16977857 |
demographic risk factors and incidence of salmonella enteritidis infection in michigan. | salmonella enteritidis is a major cause of foodborne disease worldwide. in the united states, this serotype has been associated with significant morbidity and mortality accounting for about 17% of all human salmonella infections. data on risk factors associated with serotype-specific salmonella infections have not been reported in michigan before. we carried out this study using surveillance data to identify high-risk groups and regions for s. enteritidis infection in michigan. results of this s ... | 2006 | 16972775 |
[phage types and virulence markers of clinical isolates of salmonella enteritidis]. | a set of 487 clinical isolates of salmonella enteritidis were phage typed and analyzed for virulence markers, i.e. bacterial cell surface hydrophobicity, motility, biofilm formation and the presence of a 60 kb serovar-specific virulence plasmid. the most frequent phage type was pt8 (48.3%), followed by pt13a (7.2%), pt15 (6.4%), and pt4 (4.5%). thirty-one (6.4%) strains were non-typeable. as many as 128 (26.3%) strains showed hydrophobicity in the hydrocarbon xylene adherence assay, with the hig ... | 2006 | 16970071 |
outbreak of salmonella enteritidis infections in people attending a village event in latvia. | 2006 | 16966783 | |
preventing salmonella infection. | 2006 | 16951596 | |
the adherence of salmonella enteritidis pt4 to stainless steel: the importance of the air-liquid interface and nutrient availability. | biofilm formation on stainless steel by salmonella enterica serovar enteritidis pt4 during growth in three different nutritious conditions was studied. the ability of micro-organisms to generate biofilms on the stainless steel surfaces was studied for a total period of 18 days at 20 degrees c, under three different experimental treatments: (i) growth medium (tryptone soy broth) was not refreshed (no further nutrients were provided) during the incubation period, (ii) growth medium was renewed eve ... | 2006 | 16943077 |
effect of controlled atmosphere storage, modified atmosphere packaging and gaseous ozone treatment on the survival of salmonella enteritidis on cherry tomatoes. | in recent years, outbreaks of infections associated with raw and minimally processed fruits and vegetables have been reported. the objective of this study was to analyse the growth/survival of salmonella enteritidis at spot-inoculated or stem-injected cherry tomatoes during passive modified atmosphere packaging (map), controlled atmosphere (ca) and to compare the results with those of air storage at 7 and 22 degrees c. during map, the gas composition equilibrated to 6% o2/4% co2. co2 level was m ... | 2006 | 16943034 |