Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| some characteristics of hemagglutination of certain strains of "ibv-like" virus. | 1969 | 4310341 | |
| growth in suckling-mouse brain of "ibv-like" viruses from patients with upper respiratory tract disease. | 1967 | 4298953 | |
| avian infectious bronchitis: demonstration of serum igg- and igm-neutralizing antibody by sucrose density-gradient centrifugation and mercaptoethanol reduction. | 1974 | 4215411 | |
| neutralizing and precipitating antibodies against infectious bronchitis virus in chickens. | 1974 | 4208959 | |
| some observations on the propagation of avian infectious bronchitis virus in tissue culture. | 1974 | 4207765 | |
| serial passage of 3 strains of avian infectious bronchitis virus in african green monkey kidney cells (vero). | 1973 | 4203249 | |
| intracellular development of infectious bronchitis virus. | 1967 | 4167907 | |
| the nucleic acid of infectious bronchitis virus. | 1973 | 4129679 | |
| avian infectious bronchitis virus characteristics of an inhibitor found in serum. | 1973 | 4120845 | |
| presumptive diagnosis of subclinical infections utilizing computer-assisted analysis of sequential enzyme-linked immunosorbent assays against multiple antigens. | one-hundred-seventy-two serum samples, collected sequentially from four flocks of egg- and meat-type chickens, were evaluated for antibodies to multiple infectious agents by enzyme-linked immunosorbent assay (melisa). the melisa system used provided simultaneous measurement of antibody titers against avian infectious bronchitis (ib), infectious bursal disease (bd), newcastle disease, avian encephalomyelitis and reovirus infections, and mycoplasma gallisepticum. the use of computer-generated grap ... | 1985 | 4048057 |
| infectious bronchitis virus rna d encodes three potential translation products. | 1986 | 3960740 | |
| a historical account of the diagnosis and characterization of strains of mycoplasma gallisepticum of low virulence. | numerous chicken flocks were studied beginning in 1970 because of questionable results on their serologic tests for mycoplasma gallisepticum (mg). typically a low number of hens in the flocks were positive reactors to the rapid serum plate test and rarely had hemagglutination-inhibition (hi) titers over 1:80. usually no clinical signs were observed. isolates of mg eventually were cultured from most of the flocks that exhibited that type of marginal serologic pattern. in the laboratory, the mg is ... | 1986 | 3767812 |
| an efficient ribosomal frame-shifting signal in the polymerase-encoding region of the coronavirus ibv. | the polymerase-encoding region of the genomic rna of the coronavirus infectious bronchitis virus (ibv) contains two very large, briefly overlapping open reading frames (orf), f1 and f2, and it has been suggested on the basis of sequence analysis that expression of the downstream orf, f2, might be mediated through ribosomal frame-shifting. to examine this possibility a cdna fragment containing the f1/f2 overlap region was cloned within a marker gene and placed under the control of the bacteriopha ... | 1987 | 3428275 |
| cdna cloning and sequence analysis of the gene encoding the peplomer protein of feline infectious peritonitis virus. | the peplomer gene of feline infectious peritonitis virus (fipv) strain 79-1146 was isolated from a genomic cdna library by differential hybridization with rna 2 and 3 as probes. from the nucleotide sequence a primary translation product of 1452 residues (mr 160,472) was predicted, containing an n-terminal signal sequence, a c-terminal transmembrane segment and 35 potential n-linked glycosylation sites. by s1 nuclease analysis the 5' end of the presumptive rna 2 body was located at about 30 nucle ... | 1987 | 3312491 |
| an outbreak of avian urolithiasis on a large commercial egg farm. | a significant outbreak of avian urolithiasis was observed on a large commercial egg farm. from the initial outbreak site (a single laying house), the incidence of urolithiasis slowly spread in the ensuing months to numerous other laying houses. increasing mortality associated with urolithiasis commenced during late growout to early lay and then leveled off when egg production peaked. at the height of the outbreak, mortality was typically 0.5% per week; 75% of this mortality was due to urolithias ... | 1987 | 3039970 |
| antibodies to avian infectious bronchitis virus in pakistani chickens. | using the hemagglutination inhibition test, sera from 20 diseased and 3 apparently healthy chicken flocks in pakistan were examined for antibodies to four types of avian infectious bronchitis virus (aibv). these flocks were not vaccinated against aibv. of the 900 serum samples from diseased flocks, 78 (8.7%) had antibodies to the massachusetts (m41) type, 23 (2.6%) to the arkansas type and 20 (2.2%) to the connecticut type of aibv. none had antibodies to the jmk type. none of the sera (n = 100) ... | 1987 | 3039480 |
| effect of dietary acidification on kidney damage induced in immature chickens by excess calcium and infectious bronchitis virus. | experiments were designed to evaluate the effect of dietary acidification on the development of kidney lesions induced by excess dietary calcium (ca) and gray strain infectious bronchitis virus (ibv). specific pathogen-free (spf) chicks and spf chicks inoculated with gray strain ibv were fed one of three diets: a commercial pullet grower ration (1% ca); a commercial layer ration (3.25% ca); or layer ration plus .5% ammonium chloride (acidified layer ration). gray strain ibv significantly reduced ... | 1987 | 3039478 |
| cross-reactivity among infectious bronchitis viruses in enzyme-linked immunosorbent assay. | 1987 | 3039225 | |
| effects of mutations in three domains of the vesicular stomatitis viral glycoprotein on its lateral diffusion in the plasma membrane. | the lateral mobility of the vesicular stomatitis virus spike glycoprotein (g protein) and various mutant g proteins produced by site-directed mutagenesis of the g cdna has been measured. fluorescence recovery after photobleaching results for the wild type g protein in transfected cos-1 cells yielded a mean diffusion coefficient (d) of 8.5 (+/- 1.3) x 10(-11) cm2/s and a mean mobile fraction of 75% (+/- 3%). eight mutant proteins were also examined: dtm14, lacking six amino acids from the transme ... | 1987 | 3038931 |
| systemic and local antibody responses in chickens after infection and vaccination with infectious bronchitis virus. | 1987 | 3037564 | |
| the predicted primary structure of the peplomer protein e2 of the porcine coronavirus transmissible gastroenteritis virus. | the complete nucleotide sequence of cloned cdnas containing the e2 glycoprotein-encoding region of the genome of transmissible gastroenteritis virus (tgev) has been determined. a single large translatable frame of 4.3 kb starting at 8.2 kb from the 3' end of the genome was identified. its deduced amino acid sequence contains the characteristic features of a coronavirus peplomer protein: the precursor polypeptide of tgev e2 is 1447 residues long (i.e. 285 longer than the avian infectious bronchit ... | 1987 | 3037011 |
| sequence and n-terminal processing of the transmembrane protein e1 of the coronavirus transmissible gastroenteritis virus. | sequencing of part of a clone from a transmissible gastroenteritis virus genome cdna library led to the identification of the gene encoding the e1 matrix protein. the amino acid sequence of the primary translation product predicts a polypeptide of 262 residues which shares many features with the previously characterized murine hepatitis virus and infectious bronchitis virus e1 proteins. however, n-terminal amino acid sequencing revealed that a putative signal peptide of 17 residues was absent in ... | 1987 | 3035066 |
| serological differentiation of avian infectious bronchitis field isolates using an enzyme immunoassay: presence of dutch strains in west germany. | six field virus isolates were identified as the etiologic agent of avian infectious bronchitis (ib) in west germany. a serological comparison was carried out using the m-41 strain from the massachusetts serotype and the dutch variant strains d-207, d-1466, d-3128, and d-3896 in a cross indirect immunoperoxidase test. three isolates demonstrated similarity to the m-41 strain; the remainder showed a closer antigenic relationship to the dutch variant strains. these results correspond to differences ... | 1987 | 3034228 |
| comparison of two commercial enzyme-linked immunosorbent assays and conventional methods for avian serology. | sera tested for hemagglutination-inhibition (hi) activity against newcastle disease virus (ndv) and infectious bronchitis virus (ibv) and virus-neutralizing (vn) activity against infectious bursal disease virus (ibdv) and viral arthritis (va) virus were collected from a wide variety of accessions into the diagnostic services laboratory, poultry disease research center, university of georgia. the sera were then segregated according to hi or vn titer to ndv, ibv, ibdv, or va virus and stored froze ... | 1987 | 3034226 |
| incidence, characterisation and prophylaxis of nephropathogenic avian infectious bronchitis viruses. | 1987 | 3033872 | |
| comparison of the effect of live newcastle disease vaccine clone 30 in broilers administered at day 1 or at day 7 and the effect of h120 vaccination at 17 days of age: a field experiment. | to analyse the results of a vaccination on the first day of age against newcastle disease (nd) and on the 17th day of age against infectious bronchitis (ib) resp. with spray vaccines with clone 30 and h120 vaccine. these vaccinations are compared in field circumstances with other vaccination methods. a serological examination and challenge test were used to be informed about the response and protection. from the present study the following conclusions can be drawn: clear indications are obtained ... | 1987 | 3031867 |
| [incidence of serum neutralizing antibodies to various variant strains of the infectious bronchitis virus in chickens in south württemberg]. | 1986 | 3030260 | |
| prevalence of ibv antibodies in turkey breeding flocks in israel. | 1987 | 3029940 | |
| molecular epidemiology of infectious bronchitis virus in the netherlands. | twelve dutch isolates and the m41 strain of infectious bronchitis virus (ibv), a coronavirus of chickens, were characterized by cross-neutralization and t1 finger-printing to elucidate their evolutionary relationship. the t1 fingerprinting showed that the dutch isolates formed two clusters. the first cluster contained strains h52, h120, d387, v1259, v1385 and v1397; the estimated sequence homology is 99%. cluster two comprised strains d207, d274, d212, d1466, d3128 and d3896, which have about 95 ... | 1987 | 3029279 |
| serological profiles of commercial broiler breeders and their progeny. 1. infectious bronchitis virus. | serum samples were collected from broiler breeders and their 1-day-old, 2-week-old, and 5-week-old progeny from different regions of the united states. individual samples were tested by hemagglutination-inhibition (hi) against six infectious bronchitis virus (ibv) strains: massachusetts 41 (mass), h52, connecticut 46 (conn), arkansas 99, se17, and jmk. the use of multiple strains to test broiler flocks resulted in the detection of seroconversions to conn and jmk vaccination that were not detecte ... | 1986 | 3028356 |
| standardization and application of the enzyme-linked immunosorbent assay for infectious bronchitis. | the indirect enzyme-linked immunosorbent assay (elisa) was used to detect antibody to infectious bronchitis virus in chickens. the serum-neutralization test for infectious bronchitis (snib) was used as a reference serologic test. the elisa proved to be useful for monitoring antibody responses following vaccination of leghorn chicks. the titers obtained with the elisa and snib showed an overall correlation, but results suggest the involvement of different antibody classes. | 1986 | 3028354 |
| effects of infectious bronchitis virus (arkansas strain) on laying chickens. | seventy-seven-week-old white leghorn layers were inoculated intraocularly with the arkansas strain of infectious bronchitis virus (aibv) to study the effects of the virus on egg production and on antibody response of the birds. infected hens laid fewer eggs than the controls, and those eggs weighed less than eggs laid by controls. further, the shell quality and internal quality of eggs laid by infected birds were inferior. the serum hemagglutination-inhibition (hi) titers of infected birds incre ... | 1986 | 3028350 |
| completion of the sequence of the genome of the coronavirus avian infectious bronchitis virus. | the nucleotide sequence determination of the genome of the beaudette strain of the coronavirus avian infectious bronchitis virus (ibv) has been completed. the complete sequence has been obtained from 17 overlapping cdna clones, the 5'-most of which contains the leader sequence (as determined by direct sequencing of the genome) and the 3'-most of which contains the poly(a) tail. approximately 8 kilobases at the 3' end of this sequence have already been published. these contain the sequences of mr ... | 1987 | 3027249 |
| [immunogenicity of inactivated, multivalent ib/nd oil emulsion vaccine in hybrid laying and fattening animal flocks]. | 1986 | 3026770 | |
| the application of the elisa to the diagnosis and control of avian encephalomyelitis. | an elisa for measuring serum antibody against avian encephalomyelitis virus (aev) was evaluated for its application to the diagnosis and control of avian encephalomyelitis (ae). a scoring system was developed for this elisa (ae elisa-index) so that the overall level of antibody in the flock could be presented in a single, convenient number. during suspected outbreaks of disease thought to have been caused by aev infection, the ae elisa-index increased in sequential serum samples. high levels of ... | 1986 | 3026302 |
| comparison of the spike precursor sequences of coronavirus ibv strains m41 and 6/82 with that of ibv beaudette. | the nucleotide sequences of the spike precursor genes of infectious bronchitis virus strains m41 and 6/82 have been determined and compared with that of the beaudette strain which we have previously sequenced. the two massachusetts strains, m41 and beaudette, were found to be remarkably similar, having only 3.7% of the amino acids different. the situation with 6/82, one of the new field isolates, is quite different and this strain had 13.8% of its amino acids different from beaudette. the differ ... | 1986 | 3025348 |
| development of in vitro tests for detection of extraneous agents. | the use of animals in tests to detect extraneous agents is not only undesirable from the ethical viewpoint but also because of the expense and length of time involved in such tests. we have carried out tests on a variety of potential contaminating avian pathogens to determine whether tests in chicks offer any advantage over tests in embryos or cell cultures. in many cases, but not all, in vitro tests were shown to be more sensitive. the use fluorescent antibody or enzyme-linked assays serves to ... | 1986 | 3025038 |
| the nucleotide sequence of the extreme 5' end of the avian coronavirus genome; implications for the discontinuous mrna synthesis. | 1986 | 3022236 | |
| comparison of the nephropathogenicity of four strains of infectious bronchitis virus. | experiments were conducted to characterize renal lesions in chickens induced by four strains of infectious bronchitis virus (ibv); each has been described as nephropathogenic. those strains were also compared in vaccinated and unvaccinated older chickens for nephropathogenicity. the younger birds were much more susceptible to the nephritogenic effects of the strains. all four strains produced acute renal changes consisting of tubular damage and interstitial inflammatory cell infiltration and ede ... | 1986 | 3021097 |
| the detection of antibody to avian infectious bronchitis virus by use of immunofluorescence with tissue sections of nephritic kidneys. | 1986 | 3017282 | |
| nucleotide sequence encoding the membrane protein of the ibv strain 6/82. | 1986 | 3016650 | |
| coronaviruses associated with outbreaks of transmissible enteritis of turkeys in quebec: hemagglutination properties and cell cultivation. | coronaviruses were observed by electron microscopy in the intestinal contents of turkeys in quebec flocks where repeated outbreaks of enteritis occurred. three isolates could be serially propagated in turkey embryos inoculated by the amniotic route with clarified intestinal contents. purification and concentration of viral particles contained in intestinal contents of infected embryos were achieved by precipitation with polyethylene glycol and ultracentrifugation on sucrose density gradients. th ... | 1986 | 3015116 |
| reversion to virulence of chicken-passaged infectious bronchitis vaccine virus. | serial passage of two infectious bronchitis virus (ibv) vaccine strains in chickens enhanced their capacity to increase the incidence and severity of mycoplasma synoviae (ms) airsacculitis. included in this report were the mild massachusetts-type connaught strain and the arkansas 99 vaccine strain of ibv. the connaught strain and one of two ark 99 vaccine strains passaged in chickens increased the incidence of airsacculitis markedly compared with nonpassaged virus. the other ark 99 vaccine virus ... | 1986 | 3015106 |
| nonspecific reactions in an enzyme-linked immunosorbent assay caused by binding of immunoglobulins in situ to egg-propagated infectious bronchitis virus. | high levels of nonspecific background absorbance and increased variability were found in a previously optimized enzyme-linked immunosorbent assay (elisa) for infectious bronchitis virus (ibv) antibody after changing to commercially available non-pathogen-free eggs for viral antigen production. an increase in bound viral antigen in the assay caused a proportionate increase in the nonspecific binding of the conjugate, independent of other variables, in the absence of serum. virus was propagated in ... | 1986 | 3015101 |
| rapid serological profiling by enzyme-linked immunosorbent assay. iv. association of infectious bursal disease serology with broiler flock performance. | breeder and broiler flocks were serologically evaluated using a multiple enzyme-linked immunosorbent assay (m-elisa). the serologic status of two commercial broiler-breeder flocks and their progeny was monitored, and 840 sera were promptly assessed for antibodies against six infectious agents using the m-elisa. breeder flocks were sampled at lay, and broiler chicks were hatched from fertile eggs collected on the scheduled lay date of the breeders. the broiler chicks were placed for growout as ei ... | 1986 | 3015100 |
| coronavirus ibv: removal of spike glycopolypeptide s1 by urea abolishes infectivity and haemagglutination but not attachment to cells. | urea has been used to remove the s1 spike glycopolypeptide from avian infectious bronchitis virus (ibv) strains m41 and beaudette, without removing the s2 spike-anchoring glycopolypeptide. reduction of the ph to 2.9 did not cause release of s1 although some s1 was released spontaneously from ibv beaudette at ph 7.4. virus that lacked s1 was no longer infectious or able to cause haemagglutination (ha). however, radiolabelled ibv that lacked s1 attached to erythrocytes and chick kidney cells to th ... | 1986 | 3014054 |
| coronavirus ibv: virus retaining spike glycopolypeptide s2 but not s1 is unable to induce virus-neutralizing or haemagglutination-inhibiting antibody, or induce chicken tracheal protection. | avian infectious bronchitis coronavirus (ibv) inactivated by beta-propiolactone induced partial protection of the trachea in up to 40% of chickens following one intramuscular inoculation 4 to 6 weeks prior to challenge. retention of an intact tracheal ciliated epithelium 4 days after challenge was the criterion of protection. there was no correlation between protection and serum titres of virus-neutralizing (vn) and haemagglutination-inhibiting (hi) antibody, which were maximal at about 4 weeks ... | 1986 | 3014053 |
| infectious bronchitis immunity: its study in chickens experimentally infected with mixtures of infectious bronchitis virus and escherichia coli. | the live infectious bronchitis (ib) vaccine, h120, protected chickens against intranasal challenge with a mixture of escherichia coli strains (e. coli pool) and ib virus (ibv) strains of the same (massachusetts) serotype as h120; it usually also protected against challenge with the e. coli pool and ibv strains of other serological types. when these challenge strains were themselves used as vaccines they usually protected against challenge with a mixture of the e. coli pool and an ibv strain of t ... | 1986 | 3014052 |
| cytoplasmic domains of cellular and viral integral membrane proteins substitute for the cytoplasmic domain of the vesicular stomatitis virus glycoprotein in transport to the plasma membrane. | oligonucleotide-directed mutagenesis was used to construct chimeric cdnas that encode the extracellular and transmembrane domains of the vesicular stomatitis virus glycoprotein (g) linked to the cytoplasmic domain of either the immunoglobulin mu membrane heavy chain, the hemagglutinin glycoprotein of influenza virus, or the small glycoprotein (p23) of infectious bronchitis virus. biochemical analyses and immunofluorescence microscopy demonstrated that these hybrid genes were correctly expressed ... | 1986 | 3011809 |
| coronavirus ibv glycopolypeptides: locational studies using proteases and saponin, a membrane permeabilizer. | [35s]methionine-labelled avian infectious bronchitis virus (ibv) (strain 41) and its purified protein components and virions of ibv-beaudette were incubated with 10 proteases. several proteases hydrolysed all or some of the membrane glycopolypeptide (m; mr 30k) and removed about 1.3k of peptide from the amino-(n-)-terminus plus both glycans, as determined by sds-polyacrylamide gel electrophoresis. n-terminal analysis of [3h]isoleucine-labelled m after hydrolysis by bromelain revealed that the fi ... | 1986 | 3010596 |
| coronavirus ibv: partial amino terminal sequencing of spike polypeptide s2 identifies the sequence arg-arg-phe-arg-arg at the cleavage site of the spike precursor propolypeptide of ibv strains beaudette and m41. | the spike protein of avian infectious bronchitis coronavirus comprises two glycopolypeptides s1 and s2 derived by cleavage of a proglycopolypeptide so, the nucleotide sequence of which has recently been determined for the beaudette strain (binns, m.m. et al., 1985, j. gen. virol. 66, 719-726). the order of the two glycopolypeptides within so is aminoterminus(n)-s1-s2-carboxyterminus(c). to locate the n-terminus of s2 we have performed partial amino acid sequencing on s2 from ibv-beaudette labell ... | 1986 | 3010595 |
| in ovo interference of embryo non-lethal avian infectious bronchitis viruses (ibv) with velogenic newcastle disease virus and embryo adapted ibv. | avian infectious bronchitis virus (ibv) interfered with the lethal effects of velogenic newcastle disease virus (ndv) and embryo adapted ibv in eggs previously inoculated with non-lethal ibv. greater interference was noted in eggs superinfected with embryo adapted ibv than velogenic ndv. the interference could be eliminated by treating the initial ibv with homologous anti-ibv serum. | 1986 | 3010408 |
| envelope proteins of avian infectious bronchitis virus: purification and biological properties. | immunoadsorbents, made with monoclonal antibodies, were used to purify the spike and membrane proteins of infectious bronchitis virus (ibv). the purified proteins were inoculated into rabbits to produce antisera. the rabbit anti-spike sera neutralized the infectivity of the virus whereas the anti-membrane sera did not. ibv-infected chickens produced antibodies to both the spike and membrane proteins. both these antibodies were at their highest concentration about 9-11 days after inoculation, whe ... | 1985 | 3009515 |
| predicted membrane topology of the coronavirus protein e1. | the structure of the envelope protein e1 of two coronaviruses, mouse hepatitis virus strain a59 and infectious bronchitis virus, was analyzed by applying several theoretical methods to their amino acid sequence. the results of these analyses combined with earlier data on the orientation and protease sensitivity of e1 assembled in microsomal membranes lead to a topological model. according to this model, the protein is anchored in the lipid bilayer by three successive membrane-spanning helices pr ... | 1986 | 3008826 |
| chicken embryonal vaccination with avian infectious bronchitis virus. | a commercial infectious bronchitis virus (ibv) vaccine of the massachusetts 41 strain was injected in embryonating chicken eggs on embryonation day (ed) 18. the ibv vaccine was pathogenic for embryos, and it was passaged in chicken kidney tissue culture to reduce the pathogenicity. at the 40th tissue culture passage (p40-ibv), the virus became apathogenic for the embryos. maternal antibody-positive or -negative chicks hatching from eggs injected with p40-ibv developed antibody to ibv and were pr ... | 1986 | 3008613 |
| sequence analysis of the porcine transmissible gastroenteritis coronavirus nucleocapsid protein gene. | the 3' end of the 20-kb genome of the purdue strain of porcine transmissible gastroenteritis coronavirus (tgev) was copied into cdna after priming with oligo(dt) and the double-stranded product was cloned into the psti site of the puc9 vector. one clone of 2.0-kb contained part of the poly(a) tail and was sequenced in its entirety using the chemical method of maxam and gilbert. another clone of 0.7 kb also contained part of the poly(a) tail and was sequenced in part to confirm the primary struct ... | 1986 | 3008432 |
| field observations on serological responses to an oil emulsion vaccine against avian infectious bronchitis. | 1986 | 3008406 | |
| appearance of haemagglutinability of infectious bronchitis virus following in vitro and in vivo tracheal passages. | 1985 | 3006407 | |
| an enzyme-linked immunosorbent assay method for the simultaneous measurement of antibody titer to multiple viral, bacterial or protein antigens. | a model enzyme-linked immunosorbent assay (elisa) system was developed which permits the user to evaluate replicate single sera dilutions of 46 test and control serum samples for the presence of specific antibody against up to eight different antigens in one assay. the system makes use of a transplanting device for the simultaneous transfer of aliquots of independently diluted replicate test samples along with conjugate and substrate from a serum reservoir plate or from a reagent reservoir onto ... | 1985 | 3004010 |
| cloning and sequencing of 5' terminal sequences from avian infectious bronchitis virus genomic rna. | the subgenomic rnas of the fowl coronavirus infectious bronchitis virus (ibv) form a 3' co-terminal or 'nested' set. the presence of non-contiguous (leader) sequences fused to the 5' termini of murine hepatitis virus mrnas has been demonstrated using rnase t1 oligonucleotide mapping and sequencing. the presence of a leader sequence on ibv mrna a has been demonstrated previously. in this paper the presence of a leader identical to that present on the 5' terminus of ibv mrna a is demonstrated to b ... | 1986 | 3003236 |
| pathogenesis of infectious bronchitis nephritis. 1. morphometric analysis of kidney proximal tubular epithelium in chickens. | the ultrastructure of the proximal tubular epithelial cells in chicken kidneys was examined throughout the course of an experimental infection with infectious bronchitis virus. a quantitative assessment of the structural changes in the cells was related to these in normal cells. significant alterations were detected in the membrane structures and in the mitochondria. there was a reduction in surface area of the microvillus membrane, the basolateral membrane and the apical tubular membrane. there ... | 1986 | 3003169 |
| [associated vaccination of poultry against infectious bronchitis, newcastle disease and infectious bursitis]. | the effectiveness of immunity was studied following a mixed vaccination with live vaccines against infectious bronchitis (strains h120 and h52), newcastle disease (strain la sota), and infectious bursitis (strain th75vn82). the three vaccines were applied simultaneously via the drinking water, through the spray method, and nasally. experiments were carried out with a total of 31,466 birds: broilers, growing layers, broiler parents--all without preliminary treatment with biopreparations. immunity ... | 1985 | 3002010 |
| induction of urolithiasis in single comb white leghorn pullets: effect on glomerular number. | urolithiasis was induced in an experimental group of single comb white leghorn pullets by feeding them layer ration and exposing them to nephrotrophic gray strain infectious bronchitis virus (ibv). gray strain ibv was recovered from kidney and cloacal swabs for up to 26 days after exposure to the virus. control pullets fed pullet grower ration and not exposed to gray strain ibv did not develop urolithiasis. the experimental design did not allow differentiation between the roles of layer ration a ... | 1985 | 2995958 |
| sequencing of coronavirus ibv genomic rna: three open reading frames in the 5' 'unique' region of mrna d. | the nucleotide sequence of a genomic cdna clone corresponding to the 5' terminal domain of mrna d of the beaudette strain of infectious bronchitis virus (ibv) has been determined. this region contains three open reading frames which predict polypeptides of molecular weights 6700 (6.7k), 7.4k and 12.4k. the predicted 12.4k polypeptide has a codon usage very similar to that predicted for the products of the ibv nucleocapsid, membrane and spike genes. the sequence also predicts a hydrophobic, poten ... | 1985 | 2995560 |
| the use of a random priming procedure to generate cdna libraries of infectious bronchitis virus, a large rna virus. | an efficient method for the generation of gene banks from large rna viruses is described using infectious bronchitis virus as an example. randomly primed clones have been characterized and found to be representative of the viral genome including 5' leader sequences. | 1985 | 2993338 |
| factors influencing changes in moisture content during storage of freeze-dried vaccines in vials. | previous studies have demonstrated that the water content of freeze-dried vaccines increases during storage. the reasons for these variations in water content are discussed in this paper. different possible mechanisms have been considered: microleakages at the closure sealing point, water vapour transfer through the closure (permeation, loss and uptake of water by the stopper). several types of vials and closures were studied, under different conditions of storage. regardless of the vial form se ... | 1985 | 2993309 |
| [replication characteristics and cytopathic effect of southern german field isolates of chicken infectious bronchitis virus in cell cultures and the effect of trypsin]. | 1985 | 2992447 | |
| comparative use of direct organ cultures of infected chicken tracheas in isolating avian infectious bronchitis virus. | five trials were conducted to compare four in vitro methods of isolating avian infectious bronchitis virus (ibv)-direct organ culture of infected tracheal rings (doc), inoculation of tracheal organ culture (oc), inoculation of chicken embryo, and inoculation of cultured cells. doc was prepared from tracheas of chickens experimentally inoculated with field samples. in the other methods, pooled tracheal and kidney suspensions were used to inoculate oc, chicken embryos, and cultured cells. ibv was ... | 1985 | 2992442 |
| isolation of infectious bronchitis virus from intestine and reproductive organs of laying hens with dropped egg production. | a total of 52 samples (22 cloacal swabs and 30 pooled ovaries and oviducts) from hens with a history of a 25 to 40% drop in egg production from 30 flocks were examined for the isolation of infectious bronchitis virus in embryonated chicken eggs. five isolates were obtained: three from cloacal swabs and two from reproductive organs. these isolates were identified and characterized on the basis of agar-gel-precipitation, hemagglutination, and neutralization tests and other physicochemical characte ... | 1985 | 2992441 |
| identification of field isolates of infectious bronchitis virus by interference with the la sota of strain of newcastle disease virus. | the interference phenomenon of infectious bronchitis virus (ibv) with growth of newcastle disease virus (ndv) in embryonating chicken eggs (ece) was used as a diagnostic method. fifteen field isolates obtained from presumptively infectious-bronchitis-affected chickens were analyzed by the ibv-ndv interference test. eight isolates were capable of interfering with the growth of the la sota strain of ndv, as measured by hemagglutination (ha) activity when ibv was inoculated 10 hr before ndv into ec ... | 1985 | 2992435 |
| efficacy of experimental inactivated mycoplasma gallisepticum oil-emulsion bacterin in egg-layer chickens. | six groups of white leghorn pullets were studied to determine the ability of beta-propiolactone-inactivated mycoplasma gallisepticum (mg) oil-emulsion bacterins to counteract reductions in egg production caused by mg infection. the pullets were inoculated with 0.5 ml of mg bacterin subcutaneously in the neck at about 20 weeks of age and were challenged with mg near 28 weeks of age, when they were in peak egg production. various challenge schemes with infectious bronchitis virus were used at the ... | 1985 | 2992434 |
| isolation of avian infectious bronchitis viruses in morocco including an enterotropic variant. | 1985 | 2988180 | |
| the experimental infection of chickens with mixtures of infectious bronchitis virus and escherichia coli. | by inoculating chickens intranasally with a collection of strains of infectious bronchitis virus (ibv) of the massachusetts serotype and of escherichia coli of different serotypes, a pool of viral and bacterial strains was selected which, on inoculation, consistently produced a highly lethal disease closely resembling the natural disease produced by these two organisms. the conditions for reproducing the experimental disease were not rigorous in that, within broad limits, the size of the viral a ... | 1985 | 2984320 |
| cloning and sequencing of the gene encoding the spike protein of the coronavirus ibv. | rna sequences encoding the surface projection (spike) of the coronavirus infectious bronchitis virus, strain beaudette, have been cloned into pbr322 using cdna primed with a specific oligonucleotide. a 5.3 kilobase viral insert in the clone pmb179 has been identified. the region of this clone coding for the spike gene has been sequenced by the chain termination method, and we present here the first report of dna sequence data for a coronavirus spike protein, the protein which forms the character ... | 1985 | 2984314 |
| application of the haemagglutination inhibition test to typing of infectious bronchitis virus. | 1985 | 2983480 | |
| humoral antibody response and assessment of protection following primary vaccination of chicks with maternally derived antibody against avian infectious bronchitis virus. | commercially bred chicks with maternally derived antibody to avian infectious bronchitis virus (ibv) were hatched in isolated conditions and a number vaccinated within the first three weeks of life with live ibv strain h120. humoral antibody responses were assayed by haemagglutination inhibition (hi) or neutralisation (sn) tests, and the degree of protection against challenge with the virulent massachusetts m41 strain assessed on the basis of tracheal ciliary activity four days after challenge. ... | 1985 | 2983406 |
| sequences of the nucleocapsid genes from two strains of avian infectious bronchitis virus. | cdnas prepared from viral genomic rna purified from two strains of infectious bronchitis virus (ibv) (beaudette and m41) have been cloned into pbr322. three of these clones, which contain the complete sequences of mrna a for both strains, except for the leader sequences which are only present on the subgenomic messenger rnas, have been sequenced using the dideoxy method. the sequences are similar for both strains, each containing a single long open reading frame of 1227 bases which predicts a po ... | 1985 | 2983001 |
| serological relationship among ten strains of avian infectious bronchitis virus. | ten strains of avian infectious bronchitis virus (ibv) were studied serologically by cross-neutralization test using rabbit and chicken immune sera. with the chicken sera all 10 ibv strains were antigenically related. in particular, anti-kh serum neutralized all heterologous strains except of the ishida strain; nerima strain was neutralized by all antisera except of anti-ishida serum. most cross-reactions were less or more heterologous, thus all 10 ibv strains seemed to belong to one serological ... | 1987 | 2886022 |
| infectious bronchitis: safety and protection in chickens with maternal antibody. | 1988 | 2851970 | |
| efficacy of infectious bronchitis virus vaccines against heterologous challenge. | twenty-four-week-old white leghorn layers were inoculated subcutaneously with a killed newcastle disease-infectious bronchitis (massachusetts type) virus (mibv) vaccine. twenty-eight weeks after vaccination, the birds were challenged intraocularly with the arkansas strain of infectious bronchitis virus (aibv) to determine the effects of heterologous virus exposure on egg production, egg quality and serum antibody response of the birds. the challenged hens laid significantly (p less than 0.005) f ... | 1988 | 2851866 |
| a method to rapidly convert newcastle disease virus and infectious bronchitis virus immunocomb scores into conventional hemagglutination-inhibition titers. | immunocomb scores are highly correlated to hemagglutination-inhibition (hi) titers against infectious bronchitis virus and newcastle disease virus. statistical calculations permit using an individual combscore to predict the corresponding hi titer value. tables are presented to facilitate the transformation of combscores into hi titers. | 1988 | 2848485 |
| a standardized enzyme-linked immunosorbent assay for infectious bronchitis virus: comparison with hemagglutination-inhibition and virus-neutralization assays for measuring protective antibody levels in chickens. | an enzyme-linked immunosorbent assay (elisa) was developed to measure antibodies to infectious bronchitis virus (ibv) in chickens. the results are reported in ibv standard elisa values calculated by comparing antibody levels in test sera with antibody levels in a series of standard reference sera. the ibv standard elisa values were good indicators of responses to vaccination and the immune status of experimentally challenged birds. although the assay was not serotype-specific, the sensitivity ma ... | 1988 | 2848483 |
| effect of gray strain infectious bronchitis virus and high dietary calcium on renal function of single comb white leghorn pullets at 6, 10, and 18 weeks of age. | experiments were designed to evaluate the effects of gray strain infectious bronchitis virus (ibv) and high dietary calcium (ca), alone and in combination, on renal function in pullets. eight hundred female single comb white leghorn chicks were raised on starter ration. five hundred chicks were inoculated intravenously with gray strain ibv at 4 wk of age; the remaining chicks were not exposed to ibv. at 6 wk of age, ibv-inoculated and uninoculated chicks were randomly divided into two diet treat ... | 1988 | 2847130 |
| [scanning electron microscopic studies of changes in the respiratory tract of chicks caused by an infection with the agent of infectious bronchitis]. | 1988 | 2846257 | |
| isolation of infectious bronchitis virus from a flock of racing pigeons. | 1988 | 2844141 | |
| avian infectious bronchitis diagnosis using an enzyme immunoassay on nitrocellulose. | 1988 | 2844040 | |
| cloning and sequencing of genes encoding structural proteins of avian infectious bronchitis virus. | 1988 | 2841803 | |
| the amino-terminal signal peptide on the porcine transmissible gastroenteritis coronavirus matrix protein is not an absolute requirement for membrane translocation and glycosylation. | cdna clones mapping within the first 2601 bases of the 3' end of the porcine transmissible gastroenteritis corona-virus (tgev) genome were sequenced by the method of maxam and gilbert and an open reading frame yielding a protein having properties of the matrix (m or e1) protein was identified. it is positioned at the 5' side of the nucleocapsid (n) gene from which it is separated by an intergenic stretch of 12 bases. the deduced m protein comprises 262 amino acids, has a molecular weight of 29,5 ... | 1988 | 2841792 |
| identification of recent infectious bronchitis virus isolates that are serologically different from current vaccine strains. | three 1986 infectious bronchitis virus (ibv) isolates were serotyped by a hemagglutination-inhibition method and were found to be serologically different from the seven strains currently used in regular and specially licensed vaccines in the united states (mass 41, h52, h120, conn 46, fla 18288, jmk, and ark 99) and from 13 other reference ibv strains. the recent isolates were from layer flocks that had histories of egg-production declines and shell-quality problems. two of the isolates, one eac ... | 1988 | 2840885 |
| a comparison of infectious bronchitis virus hemagglutination-inhibition test procedures. | replicate hemagglutination-inhibition (hi) tests of selected convalescent and hyperimmune infectious bronchitis virus (ibv) antisera were run against five ibv antigens: massachusetts 41, connecticut 46, h52, arkansas 99, and jmk. six different hi test procedures were included in this comparison to evaluate the effect of procedural differences on hi titers. the results were similar among procedures to the extent that positive and negative reactions were differentiated by each procedure with each ... | 1988 | 2840883 |
| comparative nephropathogenicity of infectious bronchitis virus in bursectomized and nonbursectomized chickens. | a study was conducted to compare the responses of bursectomized (bx) and nonbursectomized (non-bx) specific-pathogen-free male chickens to australian t strain of infectious bronchitis virus. four chickens from each group were killed at 3, 5, 7, 14, 21, and 28 days after conjunctival inoculation of the virus. clinical signs and gross and microscopic lesions were more severe in bx than in non-bx chickens. the non-bx chickens had linear igg deposits along the renal tubules. virus-neutralizing titer ... | 1988 | 2840842 |
| a dot-immunobinding assay for infectious bronchitis virus. | common whatman filter paper grade 1 and nitrocellulose membrane were compared for their sensitivity in a dot-immunobinding assay for detection of serum antibody titers to arkansas avian infectious bronchitis virus (aibv). for a blue to purple color detection, serum antibodies were bound to aibv antigen adsorbed on the filter-paper discs or nitrocellulose membrane. rabbit anti-chicken igg horseradish-peroxidase (hrp) conjugate and hydrogen peroxide with 4-chloro-1-naphthol (hrp-color development ... | 1988 | 2838014 |
| a study of breeder vaccination programs and problems in the broiler progeny in saskatchewan utilizing enzyme-linked immunosorbent assay. | a survey of antibodies against infectious bursal disease virus (ibdv), infectious bronchitis virus (ibv), newcastle disease virus (ndv), and reovirus (rv) was conducted in broiler-breeder flocks and selected progeny broiler flocks utilizing the enzyme-linked immunosorbent assay. marked differences in antibody titers between different breeder flocks were related to differences in vaccination programs. poor performance in some progeny broiler flocks was related to low antibody titers against ibdv ... | 1988 | 2838013 |
| effect of repeated infections of chickens with infectious bronchitis viruses on the specificity of their antibody responses. | 1988 | 2837864 | |
| evolution of avian coronavirus ibv: sequence of the matrix glycoprotein gene and intergenic region of several serotypes. | we have sequenced 200 to 240 bases of the matrix (m) glycoprotein gene of 23 strains of infectious bronchitis virus (ibv) representing the a (d207), b (d3896), c (d3128), d (d212), massachusetts (mass), uk11 and uk12 serotypes. the bases examined code for the external, hydrophilic region and the first membrane-embedded hydrophobic region of m, both regions comprising approximately 20 amino acids. as predicted from protein mr studies the a/d and b/c serotypes had two and one potential glycosylati ... | 1988 | 2832526 |
| an infectious bronchitis virus isolated from chickens experiencing a urolithiasis outbreak. i. in vitro characterization studies. | an infectious bronchitis virus (ibv) strain isolated from commercial layers experiencing urolithiasis was 50-100 nm in size and possessed widely spaced, club-shaped surface projections. it was sensitive to lipid solvents and exhibited responses characteristic of ibv when exposed to heat, divalent cations, and trypsin. reciprocal virus-neutralization tests demonstrated it to be closely related to gray, jmk, delaware 2868, and delaware 2897 strains of ibv. | 1987 | 2831871 |
| effects of avian infectious bronchitis virus (arkansas strain) on vaccinated laying chickens. | twenty-four-week-old white leghorn layers were inoculated subcutaneously with a killed newcastle-infectious bronchitis (massachusetts type) virus (mibv) vaccine. the birds were challenged 194 days later intraocularly with arkansas strain of infectious bronchitis virus (aibv). the challenged hens laid significantly (p less than 0.005) fewer eggs than the unchallenged layers, and the eggs laid by the challenged groups weighed significantly less (p less than 0.001) than those laid by the unchalleng ... | 1987 | 2831869 |
| identification of a new gene product encoded by mrna d of infectious bronchitis virus. | 1987 | 2829563 | |
| expression of ibv spike protein by a vaccinia virus recombinant. | 1987 | 2829523 | |
| the complete nucleotide sequence of avian infectious bronchitis virus: analysis of the polymerase-coding region. | 1987 | 2829522 |