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cytogenetic and molecular identification of a wheat-leymus mollis alien multiple substitution line from octoploid tritileymus x triticum durum.leymus mollis (trin.) pilger (nsnsxmxm, 2n = 28), a wild relative of common wheat, possesses many traits that are potentially valuable for wheat improvement. in order to exploit and utilize the useful genes of l. mollis, we developed a multiple alien substitution line, 10dm50, from the progenies of octoploid tritileymus m842-16 x triticum durum cv. d4286. genomic in situ hybridization analysis of mitosis and meiosis (metaphase i), using labeled total dna of psathyrostachys huashanica as probe, s ...201424938601
the effects of chronic gamma irradiation on oxidative stress response and the expression of anthocyanin biosynthesis-related genes in wheat (triticum aestivum).to investigate the mechanisms of adaptation and tolerance to ionizing radiation using chronic radiation in wheat.201424937373
peg-mediated osmotic stress induces premature differentiation of the root apical meristem and outgrowth of lateral roots in wheat.water stress is one of the major environmental stresses causing growth retardation and yield loss of plants. in the past decades, osmotic adjustment, antioxidant protection, and stomatal movement have been extensively studied, but much less attention has been paid to the study of root system reprogramming to maximize water absorption and survival under water stress. here, it is shown that polyethylene glycol (peg)-simulated mild and moderate osmotic stress induced premature differentiation of th ...201424935621
[cloning of tae-mir156 precursor gene and sequence polymorphisms of tae-mir156 targeted taspl17].squamosa-promoter binding protein (sbp)-box genes, encoding plant-specific transcription factors, play an important role in plant development. some members of the sbp-box gene family are regulated by mir156. in this study, we cloned the tae-mir156 precursor gene, which could form a stem loop after its transcription. sequence analysis showed that taspl3 and taspl17 had putative targets of tae-mir156 among the ten wheat sbp-box genes. the diploid donor species of hexaploid common wheat (triticum a ...201424929518
whole-genome qtl analysis for magic.an efficient whole genome method of qtl analysis is presented for multi-parent advanced generation integrated crosses. multi-parent advanced generation inter-cross (magic) populations have been developed for mice and several plant species and are useful for the genetic dissection of complex traits. the analysis of quantitative trait loci (qtl) in these populations presents some additional challenges compared with traditional mapping approaches. in particular, pedigree and marker information need ...201424927820
characterization of the multigene family tahkt 2;1 in bread wheat and the role of gene members in plant na(+) and k(+) status.a member of the tahkt2;1 multigene family was previously identified as a na(+) transporter with a possible role in root na(+) uptake. in the present study, the existing full-length cdna of this member was used as a basis to query the international wheat genome survey sequence to identify all members of the tahkt2;1 family. individual tahkt2;1 genes were subsequently studied for gene and predicted protein structures, promoter variability, tissue expression and their role in na(+) and k(+) status ...201424920193
biochemical and functional properties of wheat gliadins: a review.gliadins account for 40-50% of the total storage proteins of wheat and are classified into four subcategories, α-, β-, γ-, and ω-gliadins. they have also been classified as ω5-, ω1, 2-, α/β-, and γ-gliadins on the basis of their primary structure and molecular weight. cysteine residues of gliadins mainly form intramolecular disulfide bonds, although α-gliadins with odd numbers of cysteine residues have also been reported. gliadins are generally regarded to possess globular protein structure, tho ...201524915383
complex phylogeny and gene expression patterns of members of the nitrate transporter 1/peptide transporter family (npf) in wheat.npf (formerly referred to as low-affinity nrt1) and 'high-affinity' nrt2 nitrate transporter genes are involved in nitrate uptake by the root, and transport and distribution of nitrate within the plant. the npf gene family consists of 53 members in arabidopsis thaliana, however only 11 of these have been functionally characterized. although homologous genes have been identified in genomes of different plant species including some cereals, there is little information available for wheat (triticum ...201424913625
lcwrky5: an unknown function gene from sheepgrass improves drought tolerance in transgenic arabidopsis.the expression of lcwrky5 was induced significantly by salinity, mannitol and cutting treatments. arabidopsis- overexpressing lcwrky5 greatly increased dehydration tolerance by regulating the expression of multiple stress-responsive genes. based on the data of sheepgrass 454 high-throughout sequencing and expression analysis results, a drought-induced gene lcwrky5 was isolated and cloned, and the biological role of the gene has not been reported until now. bioinformatics analysis showed that lcw ...201424913125
differential expression of structural genes for the late phase of phytic acid biosynthesis in developing seeds of wheat (triticum aestivum l.).in cereals, phytic acid (pa) or inositol hexakisphosphate (ip6) is a well-known phosphate storage compound as well as major chelator of important micronutrients (iron, zinc, calcium, etc.). genes involved in the late phases of pa biosynthesis pathway are known in crops like maize, soybeans and barley but none have been reported from wheat. our in silico analysis identified six wheat genes that might be involved in the biosynthesis of inositol phosphates. four of the genes were inositol tetraphos ...201424908508
genotypic variation of zinc and selenium concentration in grains of brazilian wheat lines.exploration of genetic resources for micronutrient concentrations facilitates the breeding of nutrient-dense crops, which is increasingly seen as an additional, sustainable strategy to combat global micronutrient deficiency. in this work, we evaluated genotypic variation in grain nutrient concentrations of 20 brazil wheat (triticum aestivum l.) accessions in response to zinc (zn) and zn plus selenium (se) treatment. zn and se concentrations in grains exhibited 2- and 1.5-fold difference, respect ...201424908503
assessing the effects of architectural variations on light partitioning within virtual wheat-pea mixtures.predicting light partitioning in crop mixtures is a critical step in improving the productivity of such complex systems, and light interception has been shown to be closely linked to plant architecture. the aim of the present work was to analyse the relationships between plant architecture and light partitioning within wheat-pea (triticum aestivum-pisum sativum) mixtures. an existing model for wheat was utilized and a new model for pea morphogenesis was developed. both models were then used to a ...201424907314
water consumption characteristics and water use efficiency of winter wheat under long-term nitrogen fertilization regimes in northwest china.water shortage and nitrogen (n) deficiency are the key factors limiting agricultural production in arid and semi-arid regions, and increasing agricultural productivity under rain-fed conditions often requires n management strategies. a field experiment on winter wheat (triticum aestivum l.) was begun in 2004 to investigate effects of long-term n fertilization in the traditional pattern used for wheat in china. using data collected over three consecutive years, commencing five years after the exp ...201424905909
genetic architecture of main effect qtl for heading date in european winter wheat.a genome-wide association study (gwas) for heading date (hd) was performed with a panel of 358 european winter wheat (triticum aestivum l.) varieties and 14 spring wheat varieties through the phenotypic evaluation of hd in field tests in eight environments. genotyping data consisted of 770 mapped microsatellite loci and 7934 mapped snp markers derived from the 90k iselect wheat chip. best linear unbiased estimations (blues) were calculated across all trials and ranged from 142.5 to 159.6 days af ...201424904613
a consensus map for ug99 stem rust resistance loci in wheat.this consensus map of stem rust genes, qtls, and molecular markers will facilitate the identification of new resistance genes and provide a resource of in formation for development of new markers for breeding wheat varieties resistant to ug99. the global effort to identify new sources of resistance to wheat stem rust, caused by puccinia graminis f. sp. tritici race group ug99 has resulted in numerous studies reporting both qualitative genes and quantitative trait loci. the purpose of our study w ...201424903979
transcriptome profiling of wheat differentially expressed genes exposed to different chemotypes of fusarium graminearum.the study is an overview of the behavior of the wheat transcriptome to the fusarium graminearum fungus using two different chemotypes. the transcriptome profiles of seven putative differentially expressed defense-related genes were identified by ssh and further examined using qpcr. fusarium head blight (fhb) of wheat (triticum aestivum l.), caused by several species of the fungus fusarium, is important in all wheat growing regions worldwide. the most dominant species in canada is fusarium gramin ...201424893796
a major locus for chloride accumulation on chromosome 5a in bread wheat.chloride (cl-) is an essential micronutrient for plant growth, but can be toxic at high concentrations resulting in reduced growth and yield. although saline soils are generally dominated by both sodium (na+) and cl- ions, compared to na+ toxicity, very little is known about physiological and genetic control mechanisms of tolerance to cl- toxicity. in hydroponics and field studies, a bread wheat mapping population was tested to examine the relationships between physiological traits [na+, potassi ...201424893005
how changing root system architecture can help tackle a reduction in soil phosphate (p) levels for better plant p acquisition.the readily available global rock phosphate (p) reserves may run out within the next 50-130 years, causing soils to have a reduced p concentration which will affect plant p uptake. using a combination of mathematical modelling and experimental data, we investigated potential plant-based options for optimizing crop p uptake in reduced soil p environments. by varying the p concentration within a well-mixed agricultural soil, for high and low p (35.5-12.5 mg l(-1) respectively using olsen's p index ...201524891045
transcript suppression of tagw2 increased grain width and weight in bread wheat.bread wheat (triticum aestivum l.) is a major staple crop in the world. grain weight is a major factor of grain yield in wheat, and the identification of candidate genes associated with grain weight is very important for high-yield breeding of wheat. tagw2 is an orthologous gene of rice osgw2 that negatively regulates the grain width and weight in rice. there are three tagw2 homoeologs in bread wheat, tagw2a, tagw2b, and tagw2d. in this study, a specific tagw2-rna interference (rnai) cassette wa ...201424890396
root exudation of phytosiderophores from soil-grown wheat.for the first time, phytosiderophore (ps) release of wheat (triticum aestivum cv tamaro) grown on a calcareous soil was repeatedly and nondestructively sampled using rhizoboxes combined with a recently developed root exudate collecting tool. as in nutrient solution culture, we observed a distinct diurnal release rhythm; however, the measured ps efflux was c. 50 times lower than ps exudation from the same cultivar grown in zero iron (fe)-hydroponic culture. phytosiderophore rhizosphere soil solut ...201424890330
cold priming drives the sub-cellular antioxidant systems to protect photosynthetic electron transport against subsequent low temperature stress in winter wheat.low temperature seriously depresses the growth of wheat through inhibition of photosynthesis, while earlier cold priming may enhance the tolerance of plants to subsequent low temperature stress. here, winter wheat plants were firstly cold primed (5.2 °c lower temperature than the ambient temperature, viz., 10.0 °c) at the zadoks growth stage 28 (i.e. re-greening stage, starting on 20th of march) for 7 d, and after 14 d of recovery the plants were subsequently subjected to a 5 d low temperature s ...201424887010
whole-genome discovery of mirnas and their targets in wheat (triticum aestivum l.).micrornas (mirnas) are small, non-coding rnas playing essential roles in plant growth, development, and stress responses. sequencing of small rnas is a starting point for understanding their number, diversity, expression and possible roles in plants.201424885911
a novel genetic map of wheat: utility for mapping qtl for yield under different nitrogen treatments.common wheat (triticum aestivum l.) is one of the most important food crops worldwide. wheat varieties that maintain yield (yd) under moderate or even intense nitrogen (n) deficiency can adapt to low input management systems. a detailed genetic map is necessary for both wheat molecular breeding and genomics research. in this study, an f6:7 recombinant inbred line population comprising 188 lines was used to construct a novel genetic map and subsequently to detect quantitative trait loci (qtl) for ...201424885313
wheat in the mediterranean revisited--tetraploid wheat landraces assessed with elite bread wheat single nucleotide polymorphism markers.single nucleotide polymorphism (snp) panels recently developed for the assessment of genetic diversity in wheat are primarily based on elite varieties, mostly those of bread wheat. the usefulness of such snp panels for studying wheat evolution and domestication has not yet been fully explored and ascertainment bias issues can potentially affect their applicability when studying landraces and tetraploid ancestors of bread wheat. we here evaluate whether population structure and evolutionary histo ...201424885044
differential expression of molybdenum transport and assimilation genes between two winter wheat cultivars (triticum aestivum).molybdenum (mo) is an essential trace element for higher plants. winter wheat cultivar 97003 has a higher mo efficiency than 97014 under mo-deficiency stress. mo efficiency is related to mo uptake, transfer and assimilation in plants. several genes are involved in regulating mo uptake, transfer and assimilation in plants. to obtain a better understanding of the aforementioned difference in mo uptake, we have conducted a hydroponic trail to investigate the expression of genes related to mo uptake ...201424880579
identification of quantitative trait loci for abscisic acid responsiveness in the d-genome of hexaploid wheat.in crop species such as wheat, abiotic stresses and preharvest sprouting reduce grain yield and quality. the plant hormone abscisic acid (aba) plays important roles in abiotic stress tolerance and seed dormancy. in previous studies, we evaluated aba responsiveness of 67 aegilops tauschii accessions and their synthetic hexaploid wheat lines, finding wide variation that was due to the d-genome. in this study, quantitative trait locus (qtl) analysis was performed using an f2 population derived from ...201424877675
cytoplasmic effects on dna methylation between male sterile lines and the maintainer in wheat (triticum aestivum l.).male sterile cytoplasm plays an important role in hybrid wheat, and three-line system including male sterile (a line), its maintainer (b line) and restoring (r line) has played a major role in wheat hybrid production. it is well known that dna methylation plays an important role in gene expression regulation during biological development in wheat. however, no reports are available on dna methylation affected by different male sterile cytoplasms in hybrid wheat. we employed a methylation-sensitiv ...201424875418
α-amylase from wheat (triticum aestivum) seeds: its purification, biochemical attributes and active site studies.glycosylated α-amylase from germinated wheat seeds (triticum aestivum) has been purified to apparent electrophoretic homogeneity with a final specific activity of 1,372 u/mg. the enzyme preparation when analysed on sds-page, displayed a single protein band with mr 33 kda; superdex 200 column showed mr of 32 kda and ms/ms analysis further provided support for these values. the enzyme displayed its optimum catalytic activity at ph 5.0 and 68 °c with an activation energy of 6.66 kcal/mol and q10 1. ...201424874349
hypervirulent diuraphis noxia (hemiptera: aphididae) biotype sam avoids triggering defenses in its host (triticum aestivum) (poales: poaceae) during feeding.in the molecular arms race between aphids and plants, both organisms rely on adaptive strategies to outcompete their evolutionary rival. in the current study, we investigated the difference in elicited defense responses of wheat (triticum aestivum l.) near-isogenic lines with different dn resistance genes, upon feeding by an avirulent and hypervirulent diuraphis noxia kurdjumov biotype. after measuring the activity of a suite of enzymes associated with plant defense, it became apparent that the ...201424874154
the wheat aquaporin gene taaqp7 confers tolerance to cold stress in transgenic tobacco.aquaporin proteins (aqps) have been shown to be involved in abiotic stress responses. however, the precise role of aqps, especially in response to cold stress, is not understood in wheat (triticum aestivum). in the present study, quantitative real time polymerase chain reaction (qrt-pcr) analysis revealed that taaqp7 expression increased in leaves, but decreased in roots after cold treatment. expression of taaqp7 in tobacco plants resulted in increased root elongation and better growth compared ...201424873035
alkaloids from marine sponges as stimulators of initial stages of development of agricultural plants.damirone a (1), damirone b (2), makaluvamine g (3), debromohymenialdisine (4), and dibromoagelaspongin (5) were examined for their ability to stimulate growth of seedling roots of barley (hordeum vulgare l.), buckwheat (fagopyrum esculentum moench), corn (zea mays l.), soy (glycine max (l.) merr.}, and wheat (triticum aestivum l.). it was shown that the stimulatory effects depend on the chemical structure of the alkaloids and on the plant species. compounds 1, 3, and 4 are efficient for growth o ...201424868854
genetic control of grain yield and grain physical characteristics in a bread wheat population grown under a range of environmental conditions.genetic analysis of the yield and physical quality of wheat revealed complex genetic control, including strong effects of photoperiod-sensitivity loci. environmental conditions such as moisture deficit and high temperatures during the growing period affect the grain yield and grain characteristics of bread wheat (triticum aestivum l.). the aim of this study was to map quantitative trait loci (qtl) for grain yield and grain quality traits using a drysdale/gladius bread wheat mapping population gr ...201424865506
potential for increased photosynthetic performance and crop productivity in response to climate change: role of cbfs and gibberellic acid.we propose that targeting the enhanced photosynthetic performance associated with the cold acclimation of winter cultivars of rye (secale cereale l.), wheat (triticum aestivum l.), and brassica napus l. may provide a novel approach to improve crop productivity under abiotic as well as biotic stress conditions. in support of this hypothesis, we provide the physiological, biochemical, and molecular evidence that the dwarf phenotype induced by cold acclimation is coupled to significant enhancement ...201424860799
relationship between lutein and mycotoxin content in durum wheat.levels of lutein and a number of mycotoxins were determined in seven varieties of durum wheat (triticum durum) and two varieties of common wheat (triticum aestivum) in order to explore possible relationships amongst these components. durum wheat cultivars always showed both higher lutein and mycotoxin contents than common wheat cultivars. the mycotoxins detected in both common and durum wheat cultivars were produced by the genera fusarium, claviceps, alternaria and aspergillus. fusarium was the ...201424844356
optimization of a biolistic transformation system for transfer of antifreeze gene kn2 and the bar herbicide resistance gene in common wheat.we studied the effects of different media for callus induction and differentiation, and pre-culture period of immature wheat embryo culture on biolistic transformation efficiency for including antifreeze gene kn2 and bar conferring resistance to the herbicide bialaphos. the percentage of plantlets generated from induction and differentiation media without cu2+ was lower than those cultured on differentiation media with cu2+ (71.15%) or induction media with cu2+ (68.45%) and both induction and di ...201424841792
structure-activity relationship studies of the phytotoxic properties of the diterpenic moiety of breviones.brevianes are a family of bioactive meroterpenoids originally described in fungi of the family penicillium. these compounds have attracted a great deal of interest not only because of their unusual skeleton, suggesting a mixed mevalonate and polyketide biogenetic pathway, and their unusual oxa-spiro ring fused to an α-pyrone, but also because of the bioactivities shown by many members of this family.201524839263
mrna and small rna transcriptomes reveal insights into dynamic homoeolog regulation of allopolyploid heterosis in nascent hexaploid wheat.nascent allohexaploid wheat may represent the initial genetic state of common wheat (triticum aestivum), which arose as a hybrid between triticum turgidum (aabb) and aegilops tauschii (dd) and by chromosome doubling and outcompeted its parents in growth vigor and adaptability. to better understand the molecular basis for this success, we performed mrna and small rna transcriptome analyses in nascent allohexaploid wheat and its following generations, their progenitors, and the natural allohexaplo ...201424838975
complementary epistasis involving sr12 explains adult plant resistance to stem rust in thatcher wheat (triticum aestivum l.).quantitative trait loci conferring adult plant resistance to ug99 stem rust in thatcher wheat display complementary gene action suggesting multiple quantitative trait loci are needed for effective resistance. adult plant resistance (apr) in wheat (triticum aestivum l.) to stem rust, caused by puccinia graminis f. sp. tritici (pgt), is desirable because this resistance can be pgt race non-specific. resistance derived from cultivar thatcher can confer high levels of apr to the virulent pgt race tt ...201424838645
carbaryl toxicity prediction to soil organisms under high and low temperature regimes.many studies on risk assessment of pesticides on non-target organisms have been performed based on standardized protocols that reflect conditions in temperate climates. however, the responses of organisms to chemical compounds may differ according to latitude and thus predicting the toxicity of chemicals at different temperatures is an important factor to consider in risk assessment. the toxic effects of the pesticide carbaryl were evaluated at different temperature regimes, which are indicative ...201524836932
generation of a toxa knockout strain of the wheat tan spot pathogen pyrenophora tritici-repentis.the necrotrophic fungal pathogen pyrenophora tritici-repentis causes tan spot, a major disease of wheat, throughout the world. the proteinaceous effector toxa is responsible for foliar necrosis on toxa-sensitive wheat genotypes. the single copy toxa gene was deleted from a wild-type race 1 p. tritici-repentis isolate via homologous recombination of a knockout construct. expression of the toxa transcript was found to be absent in transformants (toxa), as was toxa protein production in fungal cult ...201424831982
identification of a hidden resistance gene in tetraploid wheat using laboratory strains of pyricularia oryzae produced by backcrossing.in the process (bc3f1 generation) of backcrossing an avena isolate of pyricularia oryzae with a triticum isolate, color mutants with white mycelia were obtained. these white mutants lacked virulence on all (31/31) hexaploid and most (28/32) tetraploid wheat lines tested. in a bc4f1 population, white and black cultures segregated in a 1:1 ratio, suggesting that the mutant phenotype is controlled by a single gene. furthermore, the mycelial color was perfectly linked with avirulence in the bc4f1 po ...201424824421
readily available phosphorous and nitrogen counteract for arsenic uptake and distribution in wheat (triticum aestivum l.).elevated arsenic content in food crops pose a serious human health risk. apart from rice wheat being another main food crop is possibly cultivated on contaminated sites. but for wheat uptake mechanisms are not entirely understood especially with regard to nutrient fertilization and different moisture regimes taking into account heavy rainfall events due to climate change. here we show that especially higher p-fertilization under changing redox conditions may enhance arsenic uptake. this countera ...201424821134
spatial x-ray fluorescence micro-imaging of minerals in grain tissues of wheat and related genotypes.wheat and its related genotypes show distinct distribution patterns for mineral nutrients in maternal and filial tissues in grains. x-ray-based imaging techniques are very informative to identify genotypes with contrasting tissue-specific localization of different elements. this can help in the selection of suitable genotypes for nutritional improvement of food grain crops. understanding mineral localization in cereal grains is important for their nutritional improvement. spatial distribution of ...201424817589
biolistic transformation of wheat with centrophenoxine as a synthetic auxin.cereal crops, including bread wheat (triticum aestivum l.), are an important staple food worldwide. with a growing global population, it is evident that current crop production will not meet the rising demands being placed on modern agriculture. efforts to improve crop yield and stress-tolerance by traditional breeding are labor intensive, time consuming, and highly dependent upon the ability to capture existing and novel genetic variation from a restricted genetic pool. genetic engineering of c ...201424816669
whole-genome profiling and shotgun sequencing delivers an anchored, gene-decorated, physical map assembly of bread wheat chromosome 6a.bread wheat (triticum aestivum l.) is the most important staple food crop for 35% of the world's population. international efforts are underway to facilitate an increase in wheat production, of which the international wheat genome sequencing consortium (iwgsc) plays an important role. as part of this effort, we have developed a sequence-based physical map of wheat chromosome 6a using whole-genome profiling (wgp™). the bacterial artificial chromosome (bac) contig assembly tools fingerprinted cont ...201424813060
interactive effect of ultraviolet-b and mineral nutrients on accumulation and translocation of trace elements in wheat crop.field study was conducted in two wheat cultivars (triticum aestivum l. cv. hd 2329 and huw 234) by supplimenting uv-b irradiation with different levels of mineral nutrients in order to evaluate the accumulation and translocation of trace elements. suv-b significantly affected accumulation and translocation of most of the metals studied. application of nutrients at higher doses enhanced the accumulation of trace elements in plants and grains of both cultivars. a higher dose of nutrient along with ...201424813006
morphological, anatomical, and ultrastructural changes (visualized through scanning electron microscopy) induced in triticum aestivum by pb²⁺ treatment.lead (pb) causes severe damage to crops, ecosystems, and humans, and alters the physiology and biochemistry of various plant species. it is hypothesized that pb-induced metabolic alterations could manifest as structural variations in the roots of plants. in light of this, the morphological, anatomical, and ultrastructural variations (through scanning electron microscopy, sem) were studied in 4-day-old seedlings of triticum aestivum grown under pb stress (0, 8, 16, 40, and 80 mg pb(2+) l(-1); mil ...201424810229
a simple and versatile 2-dimensional platform to study plant germination and growth under controlled humidity.we describe a simple, inexpensive, but remarkably versatile and controlled growth environment for the observation of plant germination and seedling root growth on a flat, horizontal surface over periods of weeks. the setup provides to each plant a controlled humidity (between 56% and 91% rh), and contact with both nutrients and atmosphere. the flat and horizontal geometry of the surface supporting the roots eliminates the gravitropic bias on their development and facilitates the imaging of the e ...201424806462
comparative functional analysis of wheat (triticum aestivum) zinc finger-containing glycine-rich rna-binding proteins in response to abiotic stresses.although the functional roles of zinc finger-containing glycine-rich rna-binding proteins (rzs) have been characterized in several plant species, including arabidopsis thaliana and rice (oryza sativa), the physiological functions of rzs in wheat (triticum aestivum) remain largely unknown. here, the functional roles of the three wheat rz family members, named tarz1, tarz2, and tarz3, were investigated using transgenic arabidopsis plants under various abiotic stress conditions. expression of tarzs ...201424800811
decay of enteric microorganisms in biosolids-amended soil under wheat (triticum aestivum) cultivation.there is a growing need for better assessment of health risks associated with land-applied biosolids. this study investigated in-situ decay of seeded human adenovirus (hadv), salmonella enterica, escherichia coli, and bacteriophage (ms2) in biosolids-amended soil under wheat cultivation. the biosolids seeded with microorganisms were placed in decay chambers which were then placed in the topsoil (10 cm depth) at three different sites. sites were selected in arid wheat-growing regions of australia ...201424793663
[molecular-genetic polymorphism of wheat cell lines resistant to metabolites of g. graminis var. tritici and osmotic stress].it was analyzed polymorphism of dna loci, flanked by inverted repeats of ltr retrotransposon cassandra, in cell lines of bread wheat, resistant to the metabolites of ophiobolus root rot (g. graminis var. tritici), under osmotic stress and induced from them plant-regenerants. the differences in the polynucleotide sequences of dna at the direct and step cell selection it was identified. assessment of the level of genetic divergence showed that calluses obtained at the direct selection and calluses ...201424791474
[alleles at storage protein loci in triticum spelta l. accessions and their occurrence in related wheats].variation at eight storage protein loci was analyzed in the collection of t. spelta accessions from the national centre of plant genetic resources of ukraine, most of which are european spelts. the analysis allowed identification of seven alleles at the gli-b1 locus, five alleles at the gli-a1 and glu-b1 loci, three alleles at the gli-a3 locus, two at the gli-d1, gli-b5, glu-a1, and glu-d1 loci. the majority of alleles are encountered among common wheat cultivars, only five alleles were specific ...201424791472
comparison of the morphogenesis of three genotypes of pea (pisum sativum) grown in pure stands and wheat-based intercrops.cereal-legume intercrops represent a promising way of combining high productivity and agriculture sustainability. the benefits of cereal-legume mixtures are highly affected by species morphology and functioning, which determine the balance between competition and complementarity for resource acquisition. studying species morphogenesis, which controls plant architecture, is therefore of major interest. the morphogenesis of cultivated species has been mainly described in mono-specific growing cond ...201424790127
dissection of rye chromosomes by the gametocidal system.chromosome mutations occur in common wheat carrying a monosome of gametocidal (gc) chromosomes 2c and 3c(sat). these gc chromosomes have been known to induce chromosomal breakage in a rye chromosome 1r added to common wheat. we attempted to introduce the two gc chromosomes into the other six rye chromosome (2r to 7r) addition or substitution lines of common wheat to establish a set of chromosomal rearrangement-inducing lines for rye chromosomes. we obtained critical plants that had a pair of rye ...201324789968
new artificial diet for continuous rearing of the bean pod borer, maruca vitrata.the bean pod borer, maruca vitrata fabricius (lepidoptera: crambidae), is a serious pantropical pest of grain legumes. a suitable artificial diet is desirable for producing uniform insects for commercial purposes or research. three previously described artificial diets, 1 newly-developed artificial diet, and cowpea (vigna unguiculata (l.) walp. (fabales: fabaceae)), the natural hostplant of m. vitrata, were used for rearing m. vitrata, and the life parameters were examined. the results indicated ...201324785903
is the multicolored asian ladybeetle, harmonia axyridis, the most abundant natural enemy to aphids in agroecosystems?the multicolored asian ladybeetle, harmonia axyridis pallas (coleoptera: coccinellidae), was introduced into western europe in the late 1990s. since the late 2000s, this species has been commonly considered one of the most abundant aphid predators in most western european countries. in spite of the large amount of research on h. axyridis, information concerning its relative abundance in agroecosystems is lacking. this study aims to evaluate the abundance of h. axyridis within the aphidophage com ...201324785375
toward an understanding of mechanisms involved in non-polyphenol oxidase (non-ppo) darkening in yellow alkaline noodles (yan).asian noodles prepared from bread wheat flour darken over time due to a combination of polyphenol oxidase (ppo) activity and non-ppo effects. although the enzymatic mechanism associated with the ppo reaction is well established, the non-ppo component consists of both physical (e.g., changes in surface properties) and chemical reactions. variations in ph and solvents were used to gain a quantitative estimate of the contribution of physical and chemical components to non-ppo darkening in yellow al ...201424784975
genome-wide identification, evolution, and expression analysis of rna-binding glycine-rich protein family in maize.the rna-binding glycine-rich protein (rb-grp) family is characterized by the presence of a glycine-rich domain arranged in (gly)n-x repeats and an rna-recognition motif (rrm). rb-grps participate in varied physiological and biochemical processes especially in the stress response of plants. in this study, a total of 23 rb-grps distributed on 10 chromosomes were identified in maize (zea mays l.), and they were divided into four subgroups according to their conserved domain architecture. five pairs ...201424783971
enhanced root and shoot growth of wheat (triticum aestivum l.) by trichoderma harzianum from turkey.it is well known that trichoderma species can be used as biocontrol and plant growth promote agent. in this study, trichoderma harzianum isolates were evaluated for their growth promotion effects on wheat in greenhouse experiments. two isolates of t. harzianum were used. the experimental design was a randomized complete block with three replications. seeds were inoculated with conidial suspensions of each isolate. wheat plants grown in steriled soil in pots. t. harzianum t8 and t15 isolates incr ...201424783790
oligonucleotides replacing the roles of repetitive sequences pas1, psc119.2, pta-535, pta71, ccs1, and pawrc.1 for fish analysis.hybrids derived from wheat (triticum aestivum l.) × rye (secale cereale l.) have been widely studied because of their important roles in wheat cultivar improvement. repetitive sequences pas1, psc119.2, pta-535, pta71, ccs1, and pawrc.1 are usually used as probes in fluorescence in situ hybridization (fish) analysis of wheat, rye, and hybrids derived from wheat × rye. usually, some of these repetitive sequences for fish analysis were needed to be amplified from a bacterial plasmid, extracted from ...201424782110
resistance of callose synthase activity to free fatty acid inhibition as an indicator of fusarium head blight resistance in wheat.the fungal pathogen fusarium graminearum is the causal agent of fusarium head blight (fhb); a devastating crop disease resulting in heavy yield losses and grain contamination with mycotoxins. we recently showed that the secreted lipase fgl1, a virulence factor of f. graminearum, targets plant defense-related callose biosynthesis during wheat head infection. this effector-like function is based on a fgl1-mediated release of polyunsaturated free fatty acids (ffa) that can inhibit callose synthase ...201424781155
molecular cytogenetic characterization of a wheat - leymus mollis 3d(3ns) substitution line with resistance to leaf rust.leymus mollis (trin.) pilger (nsnsxmxm, 2n = 28), a wild relative of common wheat, possesses many potentially valuable traits that could be transferred to common wheat during breeding programs. in this study, the karyotypic constitution of a wheat - l. mollis 3d(3ns#1) disomic substitution line isolated from the f5 progeny of octoploid tritileymus m842-16 × triticum durum cv. d4286, which was designated as 10dm57, was determined using genomic in situ hybridization (gish), fluorescent in situ hyb ...201424780618
a comparison of contents of group a and b trichothecenes and microbial counts in different cereal species.concentrations of trichothecenes and the amounts of microbial biomass were compared in grain of bread wheat, durum wheat, triticale, rye, oat and barley. grain samples came from lines regionalised in poland grown under identical climatic and agricultural conditions in 2007. among the six analysed cereals, the highest mean concentration of toxic metabolites of 151.89 µg/kg was found for grain of triticum durum, whereas the lowest was for barley grain (25.56 µg/kg). the highest contamination with ...024779779
qtl conferring fusarium crown rot resistance in the elite bread wheat variety ega wylie.fusarium crown rot (fcr) is one of the most damaging cereal diseases in semi-arid regions worldwide. the genetics of fcr resistance in the bread wheat (triticum eastivum l.) variety ega wylie, the most resistant commercial variety available, was studied by qtl mapping. three populations of recombinant inbred lines were developed with this elite variety as the resistant parent. four qtl conferring fcr resistance were detected and resistance alleles of all of them were derived from the resistant p ...201424776887
species composition and diversity of parasitoids and hyper-parasitoids in different wheat agro-farming systems.insect communities depend on both their local environment and features of the surrounding habitats. diverse plant communities may enhance the abundance and species diversity of local natural enemies, which is possible due to a higher abundance and species diversity in complex landscapes. this hypothesis was tested using cereal aphid parasitoids and hyper-parasitoids by comparing 18 spring wheat fields, triticum aestivum l. (poales: poaceae), in structurally-complex landscapes (dominated by semi- ...201324773471
high-frequency generation and characterization of intergeneric hybrids and haploids from new wheat-barley crosses.hybrid plants and a high frequency of maternal haploids were obtained using an efficient wheat-barley hybridization system (with new genotype combinations) and confirmed by several cytological and molecular tools. an efficient hybridization system between wheat (triticum aestivum l.) and barley (hordeum vulgare l.) is presented on the basis of three new genotype combinations. a particularly high, 14% frequency of plant regeneration per florets was achieved in the wheat-barley genotype combinatio ...024770442
homologous haplotypes, expression, genetic effects and geographic distribution of the wheat yield gene tagw2.tagw2-6a, cloned in earlier research, strongly influences wheat grain width and tkw. here, we mainly analyzed haplotypes of tagw2-6b and their effects on tkw and interaction with haplotypes at tagw2-6a.201424766773
the role of climatic variables in winter cereal yields: a retrospective analysis.this study examined the effects of observed climate including [co2] on winter cereal [winter wheat (triticum aestivum), barley (hordeum vulgare) and oat (avena sativa)] yields by adopting robust statistical analysis/modelling approaches (i.e. autoregressive fractionally integrated moving average, generalised addition model) based on long time series of historical climate data and cereal yield data at three locations (moree, dubbo and wagga wagga) in new south wales, australia. research results s ...201524763659
chilling to zero degrees disrupts pollen formation but not meiotic microtubule arrays in triticum aestivum l.throughout the wheat-growing regions of australia, chilling temperatures below 2 °c occur periodically on consecutive nights during the period of floral development in spring wheat (triticum aestivum l.). in this study, wheat plants showed significant reductions in fertility when exposed to prolonged chilling temperatures in controlled environment experiments. among the cultivars tested, the australian cultivars kite and hartog had among the lowest levels of seed set due to chilling and their re ...201424762030
[cloning and analysis of reverse transcriptase(rt) of ty1-copia retrotransposons in dendrobium officinale].using universal primer ty1-copia retrotransposon rt,43 ty1-copia like retrotransposon rt with high heterogeneity, stop codon mutation and frameshift mutation were amplified by pcr from genomic dna of zhejiang lin'an (c15) and yunnan guangnan (a39) of dendrobium officinale. the length of these sequences varied from 260 to 266 bp, and was rich in at and consistency ranged from 47.1% to 97.7%. different c/s-acting regulatory elements induced by low temperature, heat, light, all kinds of plant growt ...201424761633
a comprehensive genome-wide study on tissue-specific and abiotic stress-specific mirnas in triticum aestivum.productivity of wheat crop is largely dependent on its growth and development that, in turn, is mainly regulated by environmental conditions, including abiotic stress factors. mirnas are key regulators of gene expression networks involved in diverse aspects of development and stress responses in plants. using high-throughput sequencing of eight small rna libraries prepared from diverse abiotic stresses and tissues, we identified 47 known mirnas belonging to 20 families, 49 true novel and 1030 ca ...201424759739
impact of wheat-leymus racemosus added chromosomes on wheat adaptation and tolerance to heat stress.adaptation of wheat (triticum aestivum l.) to high temperatures could be improved by introducing alien genes from wild relatives. we evaluated the responses of wheat-leymus racemosus chromosome introgression lines to high temperature to determine their potentiality for developing improved wheat cultivars. introgression lines and their parent chinese spring were evaluated in a growth chamber at the seedling stage and in the field at the reproductive stage in two heat-stressed environments in suda ...201424757384
contribution of predator identity to the suppression of herbivores by a diverse predator assemblage.studies manipulating predator diversity and measuring the impact on herbivore abundance have found that enhancing predator species richness often increases the strength of prey suppression. this relationship may be due to mechanisms such as complementarity or facilitation, which are considered "true" benefits of diversity because greater prey suppression is an emergent property of the multispecies predator community. or it may be due to an identity effect, an "apparent" benefit of diversity that ...201424755243
studies on wheat bran arabinoxylan for its immunostimulatory and protective effects against avian coccidiosis.wheat (triticum aestivum) bran derived polysaccharides, arabinoxylans (axs), were evaluated for their immunostimulatory and protective efficacy against eimeria infection in chickens. humoral response revealed significantly higher (p<0.05) total igs, igg and igm titers at days 7th and 14th post primary and secondary injections of sheep red blood cells in the experimental chickens administered with axs as compared to those of control group. the percent protection and daily weight gains were signif ...201224751049
the phosphotransferase system gene ptsi in the endophytic bacterium bacillus cereus is required for biofilm formation, colonization, and biocontrol against wheat sharp eyespot.natural resistance of wheat plants to wheat sharp eyespot is inadequate, and new strategies for controlling the disease are required. biological control is an alternative and attractive way of reducing the use of chemicals in agriculture. in this study, we investigated the biocontrol properties of endophytic bacterium bacillus cereus strain 0-9, which was isolated from the root systems of healthy wheat varieties. the phosphotransferase system is a major regulator of carbohydrate metabolism in ba ...201424750250
transcriptome-wide identification of bread wheat wrky transcription factors in response to drought stress.the wrky superfamily of transcription factors was shown to be involved in biotic and abiotic stress responses in plants such as wheat (triticum aestivum l.), one of the major crops largely cultivated and consumed all over the world. drought is an important abiotic stress resulting in a considerable amount of loss in agronomical yield. therefore, identification of drought responsive wrky members in wheat has a profound significance. here, a total of 160 tawrky proteins were characterized accordin ...201424748053
space allowance and barriers influence cow competition for mixed rations fed on a feed-pad between bouts of grazing.the objective of this experiment was to evaluate how feeding space allowance and provision of feed barriers interact to affect feeding and social behavior of dairy cows fed a partial mixed ration on a feed-pad. the treatments were factorial with 3 feeding space allowances (0.6, 0.75, or 1.0m of trough space per cow) and feed troughs that were either open or had head barriers that physically separated adjacent cows to reduce interactions during feeding. one hundred and forty-four holstein-friesia ...201424746131
communities of endophytic sebacinales associated with roots of herbaceous plants in agricultural and grassland ecosystems are dominated by serendipita herbamans sp. nov.endophytic fungi are known to be commonly associated with herbaceous plants, however, there are few studies focusing on their occurrence and distribution in plant roots from ecosystems with different land uses. to explore the phylogenetic diversity and community structure of sebacinales endophytes from agricultural and grassland habitats under different land uses, we analysed the roots of herbaceous plants using strain isolation, polymerase chain reaction (pcr), transmission electron microscopy ...201424743185
three-dimensional modeling and diversity analysis reveals distinct avr recognition sites and evolutionary pathways in wild and domesticated wheat pm3 r genes.the pm3 gene confers resistance against wheat powdery mildew. studies of pm3 diversity have shown that pm3 alleles isolated from southern populations of wild emmer wheat located in lebanon, jordan, israel, and syria are more diverse and more distant from bread wheat alleles than alleles from the northern wild wheat populations located in turkey, iran, and iraq. therefore, southern populations from israel were studied extensively to reveal novel pm3 alleles that are absent from the cultivated gen ...201424742072
quality characteristics of gluten free cookies prepared from different flour combinations.the present investigation was undertaken on the utilization of alternate flours/meals (rice (oryza sativa), maize (zea mays), sorghum (sorghum vulgare) and pearl millet (pennisetum glaucum) for the preparation of gluten free cookies as compared to conventional wheat (triticum aestivum) flour cookies. the physicochemical parameters, sensory qualities and functional properties of flours/cookies were studied and compared with control cookies. the blend of maize and pearl millet had best pasting qua ...201424741176
introgression of a 4d chromosomal fragment into durum wheat confers aluminium tolerance.aluminium (al(3+)) inhibits root growth of sensitive plant species and is a key factor that limits durum wheat (triticum turgidum) production on acid soils. the aim of this study was to enhance the al(3+) tolerance of an elite durum cultivar by introgression of a chromosomal fragment from hexaploid wheat (triticum aestivum) that possesses an al(3+) tolerance gene.201424737716
micrornas involving in cold, wounding and salt stresses in triticum aestivum l.micrornas (mirnas) play critical roles in post-transcriptional regulation and act as important endogenous regulators to various stresses. cold, wounding and high-salinity are three common environmental stress stimuli influencing crops growth and development. in this study, we identified 31 known mirnas and 3 novel mirnas in wheat. moreover, 19 stress-regulated mirnas using rt-qpcr data in which the effects of three stresses were surveyed from the known mirnas. among them, 16, 12 and 8 mirnas wer ...201424735552
nutrient variability in phloem: examining changes in k, mg, zn and fe concentration during grain loading in common wheat (triticum aestivum).in wheat, nutrients are transported to seeds via the phloem yet access to this vascular tissue for exudate collection and quantitative analysis of elemental composition is difficult. the purest phloem is collected through the use of aphid stylectomy with volumes of exudate collected normally in the range of 20-500 nl. in this work a new method using inductively coupled plasma mass spectroscopy (icp-ms) was developed to measure the concentration of k, mg, zn and fe in volumes of wheat (triticum a ...201424735095
identification and characterization of micrornas in the flag leaf and developing seed of wheat (triticum aestivum l.).micrornas (mirnas) regulate various biological processes in plants. considerable data are available on mirnas involved in the development of rice, maize and barley. in contrast, little is known about mirnas and their functions in the development of wheat. in this study, five small rna (srna) libraries from wheat seedlings, flag leaves, and developing seeds were developed and sequenced to identify mirnas and understand their functions in wheat development.201424734873
efficient genome-wide detection and cataloging of ems-induced mutations using exome capture and next-generation sequencing.chemical mutagenesis efficiently generates phenotypic variation in otherwise homogeneous genetic backgrounds, enabling functional analysis of genes. advances in mutation detection have brought the utility of induced mutant populations on par with those produced by insertional mutagenesis, but systematic cataloguing of mutations would further increase their utility. we examined the suitability of multiplexed global exome capture and sequencing coupled with custom-developed bioinformatics tools to ...201424728647
exogenous jasmonic acid can enhance tolerance of wheat seedlings to salt stress.jasmonic acid (ja) is regarded as endogenous regulator that plays an important role in regulating stress responses, plant growth and development. to investigate the physiological mechanisms of salt stress mitigated by exogenous ja, foliar application of 2mm ja was done to wheat seedlings for 3days and then they were subjected to 150mm nacl. our results showed that 150mm nacl treatment significantly decreased plant height, root length, shoot dry weight, root dry weight, the concentration of gluta ...201424726929
patterns of homoeologous gene expression shown by rna sequencing in hexaploid bread wheat.bread wheat (triticum aestivum) has a large, complex and hexaploid genome consisting of a, b and d homoeologous chromosome sets. therefore each wheat gene potentially exists as a trio of a, b and d homoeoloci, each of which may contribute differentially to wheat phenotypes. we describe a novel approach combining wheat cytogenetic resources (chromosome substitution 'nullisomic-tetrasomic' lines) with next generation deep sequencing of gene transcripts (rna-seq), to directly and accurately identif ...201424726045
genetic diversity and population structure analysis of european hexaploid bread wheat (triticum aestivum l.) varieties.progress in plant breeding is facilitated by accurate information about genetic structure and diversity. here, diversity array technology (dart) was used to characterize a population of 94 bread wheat (triticum aestivum l.) varieties of mainly european origin. in total, 1,849 of 7,000 tested markers were polymorphic and could be used for population structure analysis. two major subgroups of wheat varieties, gri and grii, were identified using the program structure, and confirmed by principal com ...201424718292
snp discovery for mapping alien introgressions in wheat.monitoring alien introgressions in crop plants is difficult due to the lack of genetic and molecular mapping information on the wild crop relatives. the tertiary gene pool of wheat is a very important source of genetic variability for wheat improvement against biotic and abiotic stresses. by exploring the 5mg short arm (5mgs) of aegilops geniculata, we can apply chromosome genomics for the discovery of snp markers and their use for monitoring alien introgressions in wheat (triticum aestivum l).201424716476
genetic diversity in australian wheat varieties and breeding material based on rflp data.restriction fragment length polymorphisms (rflps) have been used to characterise the genetic diversity of wheat (triticum aestivum) germplasm. one hundred and twenty-four accessions comprising all major australian wheat varieties and lines important for breeding purposes were assayed for rflps with clones of known genetic location and selected to give uniform genome coverage. the objectives of this study were to determine rflp-based genetic similarity between accessions and to derive association ...199824710883
cloning and characterization of tapp2abb"-α, a member of the pp2a regulatory subunit in wheat.protein phosphatase 2a (pp2a), a major serine/threonine protein phosphatase, consists of three subunits; a highly conserved structural subunit a, a catalytic subunit c, and a highly variable regulatory subunit b which determines the substrate specificity. although the functional mechanism of pp2a in signaling transduction in arabidopsis is known, their physiological roles in wheat remain to be characterized. in this study, we identified a novel regulatory subunit b, tapp2abb"-α, in wheat (tritic ...201424709994
characterization of a wheat pathogenesis-related protein, tabwpr-1.2, in seminal roots in response to waterlogging stress.we examined the role of pathogenesis-related protein tabwpr-1.2 in the context of molecular and physiological responses of wheat (triticum aestivum) seminal roots under waterlogging stress. two cdnas corresponding to the tabwpr-1.2 gene, tabwpr-1.2#2 and tabwpr-1.2#13 were cloned from seminal roots. these cdnas were predicted to encode proteins of 173 and 172 amino acids, respectively. in a time-course experiment, tabwpr-1.2 gene expression was highest in whole seminal roots after 1 day of water ...201424709151
central role of the flowering repressor zcct2 in the redox control of freezing tolerance and the initial development of flower primordia in wheat.as both abiotic stress response and development are under redox control, it was hypothesised that the pharmacological modification of the redox environment would affect the initial development of flower primordia and freezing tolerance in wheat (triticum aestivum l.).201424708599
overexpression of a wheat phospholipase d gene, tapldα, enhances tolerance to drought and osmotic stress in arabidopsis thaliana.phospholipase d (pld) is crucial for plant responses to stress and signal transduction, however, the regulatory mechanism of pld in abiotic stress is not completely understood; especially, in crops. in this study, we isolated a gene, tapldα, from common wheat (triticum aestivum l.). analysis of the amino acid sequence of tapldα revealed a highly conserved c2 domain and two characteristic hkd motifs, which is similar to other known pld family genes. further characterization revealed that tapldα e ...201424705986
development and discrimination of 12 double ditelosomics in tetraploid wheat cultivar dr147.as an important group in triticum, tetraploid wheat plays a significant role in the research of wheat evolution. several complete aneuploid sets of common wheat have provided valuable tools for genetic and breeding studies, while similar aneuploids of tetraploid wheat are still not well developed. here, 12 double ditelosomics developed in triticum turgidum l. var. durum cultivar dr147 (excluding ddt2b and ddt3a) were reported. hybrids between dr147 and the original double-ditelosomic ddt2b of la ...201424702066
increased micronutrient content (zn, mn) in the 3m(b)(4b) wheat - aegilops biuncialis substitution and 3m(b).4bs translocation identified by gish and fish.3m(b) triticum aestivum l. (mv9kr1) - aegilops biuncialis vis. (mvgb642) addition lines were crossed with the chinese spring ph1b mutant genotype (csph1b) to produce 3m(b)-wheat chromosome rearrangements. in the f3 generation, 3m(b)(4b) substitution lines and 3m(b).4bs centric fusions were identified with in situ hybridization using repetitive and genomic dna probes, and with ssr markers. grain micronutrient analysis showed that the investigated ae. biuncialis accession mvgb382 and the parental ...201424702063
acute toxicity test of a natural iron chelator and an antioxidant, extracted from triticum aestivum linn. (wheat grass).triticum aestivum (wheat grass) is widely used in traditional medicine to treat various diseases. previously the purified compounds and crude extract of t. aestivum were established to have iron chelation potency and antioxidant activity. so it is necessary to evaluate the toxic properties of any compound isolated from plant extract to prevent any untoward side effects. the aim of this study was to determine the acute oral toxicity level of our purified compounds, i.e. mugineic acids and methylp ...201424697628
development of a self-fertile ditelosomic line for the long arm of chromosome 4b and its characterization using ssr markers.the ditelosomic line for the long arm of chromosome 4b (4bl) of chinese spring (cs) wheat is not available because it is completely male sterile. since all deletions in the 4b short arm (4bs) cause male sterility in the homozygous condition, a male-fertility gene should be located in a distal region of 4bs. among the selfed progeny of a hybrid between a male-sterile 4bs deletion plant (4bs-8) and a japanese common wheat cultivar norin 61 (n61), we obtained self-fertile 4bs-8 homozygous deletion ...201324694394
enrichment of provitamin a content in wheat (triticum aestivum l.) by introduction of the bacterial carotenoid biosynthetic genes crtb and crti.carotenoid content is a primary determinant of wheat nutritional value and affects its end-use quality. wheat grains contain very low carotenoid levels and trace amounts of provitamin a content. in order to enrich the carotenoid content in wheat grains, the bacterial phytoene synthase gene (crtb) and carotene desaturase gene (crti) were transformed into the common wheat cultivar bobwhite. expression of crtb or crti alone slightly increased the carotenoid content in the grains of transgenic wheat ...201424692648
comparative analysis of physio-biochemical responses to cold stress in tetraploid and hexaploid wheat.the cellular changes induced by cold stress (cs) include responses that lead to oxidative stress and limits plant growth, metabolism, and productivity. in this study, responses of physio-biochemical to cs phases were comparatively studied in three genotypes of bread and durum wheats differing in sensitivity, two of them (norstar, bread wheat and gerdish, durum wheat) were tolerant to cs and the other one, srn (durum wheat) was sensitive to cs. 14-day-old seedlings were subjected to cs (12 and 24 ...201424691928
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