Publications

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differentiation of potyviruses and their strains by hybridization with the 3' non-coding region of the viral genome.nucleic acid hybridization with the 3' non-coding region of the potyvirus genome as the probe was shown to be a relatively simple means of distinguishing between distinct potyviruses and their strains. comparisons of the nucleotide sequences of potyvirus genomes (ignoring gaps) showed that the degree of identity between equivalent genes of strains was greater than 96%, while between distinct potyviruses the identity ranged from 42% to 65%, suggesting that any extended sequence could be considere ...19921551936
fauna, flora, fowl, and fruit: effects of the columbian exchange on the allergic response of new and old world inhabitants.the columbian exchange has been described as "the most important event in human history since the end of the ice age." this interchange of many species of fauna, flora, fowl, and fruits resulted in new encounters between new and old world inhabitants. prominent among these were manifestations of allergic reactions to many of the new substances. little imagination is required to reflect on what these substances, added to or detracted from both the new and old world lifestyles, habits, and diets. ...19921490627
present status of the sugarcane mosaic subgroup of potyviruses.until recently, sugarcane mosaic virus (scmv) was believed to be a single potyvirus consisting of a large number of strains, differing from each other in certain biological and antigenic properties. the use of affinity-purified polyclonal antibodies directed towards the surface-located, virus-specific amino termini of the coat proteins showed that 17 strains from australia and the united states represented four distinct potyviruses, namely johnsongrass mosaic virus (jgmv), maize dwarf mosaic vir ...19921450763
differentiation of the four viruses of the sugarcane mosaic virus subgroup based on cytopathology.a cytological comparison has been made of representative isolates of johnsongrass mosaic (jgmv), maize dwarf mosaic (mdmv), sorghum mosaic (srmv) and sugarcane mosaic (scmv) viruses. these four viruses now encompass the complex of virus strains which were formerly considered as strains of sugarcane mosaic and/or maize dwarf mosaic viruses. the structure of the cytoplasmic cylindrical inclusions induced by these viruses, together with other cytological alterations, allow the four viruses to be di ...19921450762
pcr amplification of a specific double-stranded rna region of fiji disease virus from diseased sugarcane.a 450-bp region from one species of the segmented dsrna genome of fiji disease virus (fdv) was amplified from total nucleic acid extracts of diseased plants by reverse transcription with mmlv, followed by amplification with taq dna polymerase (rt-pcr). other fdv-specific regions (c 150 bp and c 270 bp) were also amplified from the dsrna template. fdv cdna was only synthesised when the viral dsrna template was boiled and quenched with fdv-specific or random hexamer primers. the reverse transcript ...19921385465
fungal protein enrichment of residual liquor from a sugar-cane waste saccharum officinarum.the use of residual liquor obtained from cane-thrash, a sugar-cane waste saccharum officinarum, l. , as a protein source was studied. the cane-thrash was available in local sugar-mills and the fungus utilized, the aspergillus niger iz-9, was provided by the university of são paulo and derived from a strain under the number nrrl-337. the liquor was previously hydrolyzed and physicochemical analyses were performed. total nitrogen and amino acid contents were measured in the final product. the synt ...19921342170
effect of alkaline hydrogen peroxide treatment on cell wall composition and digestion kinetics of sugarcane residues and wheat straw.our objective was to characterize changes in cell wall composition and digestibility of sugarcane bagasse, pith from bagasse, and wheat straw after treatment with alkaline hydrogen peroxide (ahp). the ahp treatment solution contained 1% h2o2 (wt/vol) maintained at ph 11.5 with naoh. the h2o2 in solution amounted to 25% of the quantity of substrate treated. after treatment, residues were washed and dried. detergent fiber composition, total fiber components (neutral sugars, uronic acids, klason li ...19921328129
deficiency of toxin-binding protein activity in mutants of sugarcane clone h54-775 as it relates to disease resistance.three mutants selected from a population of sugarcane clone h54-775 that had been irradiated with 3 kr gamma-radiation all lacked toxin-binding protein activity. this activity previously had been shown to be essential for eye spot disease susceptibility and was demonstrated in the susceptible parent clone h54-775. in one mutant, the biochemical, immunochemical, and electrophoretic mobilities of the toxin-binding protein were all modified.19751203055
transfer of toxin susceptibility to plant protoplasts via the hemlmintosporoside binding protein of sugarcane. 19751169066
on the occurrence of p-type plastids in sieve elements of sugarcane short communication. 2003187392
the relationship between membrane atpase activity in sugarcane and heat-induced resistance to helminthosporoside.1. heating of susceptible sugarcane leaves (4 h at 35 degrees c) renders them resistant, for 24 h, to the effects of helminthosporoside. membrane atpase activity is reduced by 50% as a result of the heat treatment. when the leaves again become susceptible (after 24 h), membrane. atpase activity is fully restored. 2. inhibitors of membrane atpase activity protect susceptible leaves from the effects of helminthosporoside (kf, edta, and octylguanidine). 3. helminthosporoside activates (30%) membran ...1979158388
enzymatic hydrolysis of cellulosic materials by sclerotium rolfsii culture filtrate for sugar production.the hydrolysis of purified celluloses (cotton, avicel, cellulose-123, solka floc sw40) and cellulosic wastes (rice straw, sugarcane bagasse, wood powders, paper factory effluents) by sclerotium rolfsii cpc 142 culture filtrate was studied. factors which effect saccharification such as ph, temperature, enzyme concentration, substrate concentration, produce inhibition, adsorption, and inactivation of enzyme and particle size were studied. virtually no inhibition (less than 3%) of cellulose hydroly ...197938898
effects of citrus pulp in high urea rations for steers.effects of pelleted and conventional citrus pulp as a replacement for corn, with soybean meal added to keep protein comparable, were tested in rations with 5% urea and 33.33% sugarcane bagasse for fistulated steers. thus, all rations were low in readily fermented carbohydrates other than those of corn or citrus pulp. evaluation criteria were concentrations of urea in blood and of ph, ammonia, and volatile fatty acids of rumen fluid. citrus pulp for diets 1, 2, 3, and 4 was 0, 19, 38, or 55%. rum ...19766495
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