Publications

isolation and sequencing of cdna clones encoding ethylene-induced putative peroxidases from cucumber cotyledons.	a cdna library from ethephon-treated cucumber cotyledons (cucumis sativus l. cv. poinsett 76) was constructed. two cdna clones encoding putative peroxidases were isolated by means of a synthetic probe based on a partial amino acid sequence of a 33 kda cationic peroxidase that had been previously shown to be induced by ethylene. dna sequencing indicates that the two clones were derived from two closely related rna species that are related to published plant peroxidase sequences. southern analysis ...	1990	2102850
the role of oxygen in thermo-photodynamic processes leading to sunscald-like damages in green tissues.	sunscald is a physiological disorder causing, in warm climates, severe damages to green tissues. earlier studies established the essential role of heat, light and chlorophyll in the development of the disorder. in the present work, the role of oxygen in the development of the injury was investigated. green cucumber fruit were exposed to heat and light under controlled atmosphere, and the development of the injury was followed. no damage occurred in the absence of either heat or light. tissues ex ...	1991	2060853
molecular cloning of the gene for indolepyruvate decarboxylase from enterobacter cloacae.	although indole-3-acetic acid (iaa) is a well-known plant hormone, the main iaa biosynthetic pathway from l-tryptophan (trp) via indole-3-pyruvic acid (ipya) has yet to be elucidated. previous studies have suggested that iaa is produced by enterobacter cloacae isolated from the rhizosphere of cucumbers and its biosynthetic pathway may possibly be the same as that in plants. to elucidate this pathway, the iaa biosynthetic gene was isolated from a genomic library of e. cloacae by assaying for the ...	1991	2034209
stearoyl-acyl-carrier-protein desaturase from higher plants is structurally unrelated to the animal and fungal homologs.	stearoyl-acyl-carrier-protein (acp) desaturase (ec 1.14.99.6) was purified to homogeneity from avocado mesocarp, and monospecific polyclonal antibodies directed against the protein were used to isolate full-length cdna clones from ricinus communis (castor) seed and cucumis sativus (cucumber). the nucleotide sequence of the castor clone prcd1 revealed an open reading frame of 1.2 kilobases encoding a 396-amino acid protein of 45 kda. the cucumber clone pcsd1 encoded a homologous 396-amino acid pr ...	1991	2006187
gas chromatographic determination of captan, folpet, and captafol residues in tomatoes, cucumbers, and apples using a wide-bore capillary column: interlaboratory study.	an interlaboratory study of the determination of captan, folpet, and captafol in tomatoes, cucumbers, and apples was conducted by 4 laboratories using wide-bore capillary column gas chromatography with electron capture detection. the 3 fungicides were determined using the luke et al. multiresidue method modified to include additional solvent elution in the optional florisil column cleanup step used with this method. the crops were fortified with each fungicide at 3 levels per crop. mean recoveri ...	2015	1783591
complex formation of nuclear proteins with the rna polymerase i promoter and repeated elements in the external transcribed spacer of cucumis sativus ribosomal dna.	complex repetitive structures are located downstream of the transcription initiation site in the intergenic spacer (igs) of the rrna genes in cucumis sativus (cucumber). in order to show that these repetitive elements of the 5'external transcribed spacer (ets) are probably involved in transcriptional regulation as protein binding sites dna-protein binding assays were carried out. the same proteins that recognize two binding sites in the promoter region analysed (upstream binding element between ...	1992	1641334
transformation of cucumber tissues by microprojectile bombardment: identification of plants containing functional and non-functional transferred genes.	the microprojectile bombardment method was used to transfer dna into embryogenic callus of cucumber (cucumis sativus), and stably transformed cucumber plant lines were obtained. a total of 107 independently regenerated cucumber plants were assayed for the presence and expression of the transferred nos-nptii gene (encoding nopaline synthase-neomycin phosphotransferase ii). genomic blot hybridization analyses showed that a high percentage (16%) of the cucumber plants were transformed with nos-npti ...	1992	1324874
fungicide residues. part v. determination of residues of chloraniformethan in grain and cucumbers by gas chromatography.		1976	1259174
the enzymic formation of long chain aldehydes and alcohols by alpha-oxidation of fatty acids in extracts of cucumber fruit (cucumis sativus).	1. an enzyme system that catalyses the alpha-oxidation of fatty acids to shorter chain products is present in acetone powders of cucumber fruits. 2. in the absence of nad+, the predominant product from palmitic acid is pentadecanal. addition of nad+ gives rise to a homologous series of n-alkanals, the concentrations of which are in the same order as that reported in the volatile products formed on homogenization of cucumbers, i.e. c15 greater than c14 greater than c13 greater than c12. 3. pentad ...	1976	1252478
collaborative study of the determination of endosulfan, endosulfan sulfate, tetrasul, and tetradifon residues in fresh fruits and vegetables.	a method for the determination of endosulfan i, endosulfan ii, endosulfan sulfate, tetrasul, and tetradifon residues in fresh fruits and vegetables was studied collaboratively. the method consists of extraction of the product with acetonitrile, ether and elution from a florisil column with mixtures of hexane, methylene chloride, and acetonitrile, and determination of the residues by electron capture gas-liquid chromatography. this method was studied by 8 collaborators, using apples and cucumbers ...	1976	1249036
[on the phenolic acids of vegetables. ii. hydroxycinnamic acids and hydroxybenzoic acids of fruit and seed vegetables (author's transl)].	fruits of solanaceae (tomatoes, eggplant, and sweet peppers) almost exclusively contain hydroxycinnamic acid derivatives with caffeic acid dominating. fruits of cucurbitaceae (cucumbers, melons, pumpkins, and zucchini) are extraordinary, because they show very low concentrations of phenolic acids (up to 10 mg/kg) accumulated in the peels. peas and broad beans have relatively small contents of phenolic acids too. their husks show like beans considerable concentrations of hydroxycinnamic acid deri ...	1975	1224795
[influence of pre-sowing gamma-irradiation on the biology and fruitfulness of cucumbers].		2006	1208812
[electrophoretical differentiation and classification of proteins. vi. disc-electrophoresis and isoelectrical focossing of proteins and enzymes from tomatoes, cucumber, sugar-maize and onions (author's transl)].	acetone-dry powders which are excellently suited for the enrichment of proteins and partially also for enzymes, have been extracted with buffers. separation was accomplished using disc-electrophoresis and isoelectrical focusing. the protein distribution patterns of fleshy tomatoe varieties largly correspond to each other. in the protein distribution patterns of cucumbers, sugar-maize and onions, the protein pherograms indicate sort-dependency. the zymograms partially differ very much. this can n ...	1975	1202837
viability of vibrio cholerae biotype el tor and of cholera phage on vegetables.	different vegetables were contaminated with sewage water containing large amounts of el tor vibrios, and the viability of these vibrios was studied under defined conditions of storage. during the dry season (summer) in jerusalem, the el tor vibrios survived for up to 24 hr on parsley, 24 to 30 hr on tomatoes and carrots, 24 to 48 hr on cucumbers, peppers and okra and two to three days on lettuce, when the vegetables were stored at room temperature. the survival time was longer during the rainy s ...	1975	1184361
effects of tentoxin on enzymic activities in cucumber and cabbage cotyledons.	tentoxin, produced by alternaria alternata (fr.) keissl. causes severe variegated chlorosis in germinating seedlings of certain dicotyledonous species. however, it does not impair radicle and hypocotyl elongation or cotyledon expansion. effects of the toxin on the activity of selected enzymes from both chloroplasts and cytoplasm were determined. cucumber (cucumis sativus l.), which is highly sensitive to tentoxin and cabbage (brassica oleracea l.), which is resistant, were used as test plants. t ...	1975	1171367
localization and metabolites of aminotriazines and chlorophenoxyacetic acid in cucumber (cucumis sativus, l.).		1975	1063675
similarities among ribosomal rna's of angiospermae and gymnospermae.	competitive hybridization of ribosomal rnas was used to estimate similarities of nucleotide sequences between species of angiospermae and gymnospermae. similarities have been measured with respect to cucumis sativus. in angiospermae the nucleotide sequences are highly conserved except in the species of the family of compositae, where the percentages of similarity are clearly lower. in the gymnospermae the lowest similarity has been observed with torreya californica. a relationship is hypothesize ...	1976	1011263
organochlorine insectide residues in vegetables of the kitakyushu district, japan--1971-74.	the residue levels of organochlorine insecticides bhc, ddt, endrin, and dieldrin in the kitakyushu district, japan, were monitored from 1971 to 1974. agricultural uses of these insecticides were banned in 1970. bhc isomers, alpha, beta, gamma, and delta-bhc were detected in all vegetable samples taken; beta-bhc residue appeared in the highest levels. the proportions of each bhc isomer in total bhc residues were much different from those in the technical product. average residue levels of alpha, ...	1976	1005058
residue analysis of chlorflurenol in cucumbers.		2000	1002925
[determination of morestane in water, soil and cucumbers by thin-layer chromatography].		2000	969385
[signal trait of cucumbers].	a mutant of cucumber characterized by the absence of nappiness on all the organs of the plant was found at birjuchekutian station in 1970. this character is recessive and is manifested in the second generation showing a 3:1 ratio of nappy and plain plants. on the basis of this mutant, forms of cucumber without nappiness were obtained. plain plants are distinguished especially well at the initial stages of development when the first leaf appears. they are viable and productive, grow perfectly wel ...	2008	929676
extraction and gas-liquid chromatographic determination of hexachlorophene from several plant tissues.	conditions are described for extraction, cleanup, derivatization, detection, and quantitation of hexachlorophene (hcp) residues from several types of plant material. wet plant tissue was homogenized and extracted with ethyl ether, dried, and methylated with excess diazomethane. samples were precleaned by column chromatography on silica gel with benzene-petroleum ether (1 + 1), and then 1-10 microliter concentrated sample was chromatographed on a 6' column of 3% se-30 on 80-100 mesh gas-chrom q. ...	1977	893328
[thin-layer chromatographic determination of chlordimeform in cucumbers and apples].	the author describes two methods for the thin-layer chromatographic determination of chlordimeform residues in cucumbers and apples. chlordimeform is determined alone or together with its relevant metabolites after conversion into 4-chloro-o-toluidine. the limit of detection is 0.1 and 0.05 p.p.m., respectively. the rate of recovery is 76-85 and 90-105%, respectively. studies on the stability of the residues and on the metabolism and distribution of chlordimeform in cucumbers are discussed.	1977	876348
[patulin in foods of vegetable origin. 2. several kinds of fruit and vegetables and fruit and vegetable products (author's transl)].	patulin was found in fruit with spontaneous brown rot (bananas, pineapples, grapes, peaches, apricots) as well as in moldy compots and in sallow-thorn juice. fruit, vegetables and fruit and vegetable product were artificially infected with penicillium expansum, p. urticae and byssochlamys nivea; patulin was subsequently found in peaches, apricots, greengages, bananas, strawberries, honeydew melons, tomatoes, red and green paprika, cucumbers and carrots; in several kinds of compot, in tomato juic ...	1977	857490
[determination of patulin in foodstuffs part ii: determination of patulin in tomatoes, pears, apples cucumbers and plums (author's transl)].	an analytical method for the determination of patulin in tomatoes, pears, apples, cucumbers and plums is described. the determination limit of the method is 40 mug patulin/kg. with additions between 100-500 mug patulin/kg the recovery rates lie between 86--95%. after a pre-purification of the crude extract by column chromatography and liquid-liquid extraction the patulin is determined thin-layer chromatographically by reflectance measurement at 273 nm.	1977	855502
the enzymic cleavage of linoleic acid to c9 carbonyl fragments in extracts of cucumber (cucumis sativus) fruit and the possible role of lipoxygenase.	1. homogenates and acetone powders of cucumber fruits catalyse the enzymic conversion of linoleic acid to aldehyde and oxoacid fragments in high yield, up to 60% with acetone powder extracts. 2. the major products are trans2-nonenal--a major component of the characteristic odour of cucumber--and 9-oxononanoic acid. 3. the cleavage reaction is a heat-labile, aerobic process, optimal at ph 6 (approx.). 4. substrate specificity studies indicate that a lipoxygenase-type of reaction is involved in th ...	1976	820376
effects of brine treatment on pesticide residues during recycling of pickling brine.	the effects of 2 pickle brine recycling treatments on residues of selected insecticides, herbicides, and fungicides are presented. residues were determined in raw and brined cucumbers, in untreated brine, and in brine following pasteurization or chemical (naoh) treatment. the samples were extracted with acetone, partitioned into methylene chloride, cleaned up by gel permeation chromatography, and quantitatted by gas-liquid chromatography using nitrogen/phosphorus, electron capture, and flame pho ...	1978	730651
[mode of action and inhibition of polygalacturonase covalently bound to polysaccharide and glass carriers].	endo-polygalacturonase (ec 3.2.1.15.) from aspergillus spec. is much changed as far as its mode of action and the interaction with vegetable inhibitors of pectinase (from green beans and cucumbers) are concerned when it is covalently bound to insoluble carriers (sepharose, cellulose powder, macroporous glass and nonporous ballotinis). whereas a 2% degradation of substrate by the soluble enzyme caused a 50% decrease of viscosity of citrus pectic acid, the comparable degradation of substrate was i ...	1978	692641
analysis of plant genomes. iv. isolation and characterization of satellite dna components from two dicotyledons cucumber (cucumis sativus) and radish (raphanus sativus).	satellite dna fractions from cucumber and radish, two plants having low dna contents and relatively small chromosomes, were isolated and characterized. reassociation studies of satellite and total nuclear dna showed that the satellite fractions in these two plants contain most of the rapidly reassociating dna. cucumber satellite i was found to contain one major component (70% of the total satellite) having a density of 1.706 g/cm3 and a tm of 90.5 degrees c and a minor component with a density o ...	1978	688067
absence of parallelism between polyamine and nucleic acid contents during induced growth of cucumber cotyledons.	the cytokinins (benzyladenine or benzyladenosine) decreased spermidine and spermine contents despite increasing putrescine content, when administered to isolated cotyledons of cucumis sativus l. var. guntur in organ culture. kcl decreased putrescine contents, although marginally increasing polyamine contents. the cytokinins and/or kcl augmented nucleic acid biosynthesis and accumulation, resulting in enhanced growth and differentiation of the isolated cotyledons. these observations show that pol ...	1978	656071
[possible use of ultraviolet fluorescence for detecting protein conformational transitions in intact tissues].	spectra of fluorescence (sf) excited by the light with the wave length of 260 nm were measured with the aim of revealing the character of structural reconstructions in water-dificient plant cells. hypocotyls of cucumis sativus l. seedlings during soil draught served as the objects of study. with an increase of water deficiency the sf maximum is shifted to 5-8 nm to the long wave region. in wilted plant tissues free triptophane was accumulated whose sf maximum was shifted to the long wave region ...	2013	623823
occurrence of patulin in fruit and vegetables.	in different varieties of apples and pears suffering from brown rot, patulin was found in about 50 p. 100 of samples investigated (about 120). patulin levels as high as 1 g/kg rotten material were found 2-3 days after the fruit was removed from the cold store where it had been stored for 5 months. a significant diffusion into the healthy plant tissue was not observed in apples, but in peaches, tomatoes and pears. vegetable products with natural patulin content: apples, pears, peaches, apricots, ...	1977	613910
[interaction of ascorbate oxidase with inorganic anions].	from the peelings of cucumber cucumis sativus and marrow squash cucurbita pepo var. giramontia highly purified ascorbate oxidase preparations were obtained. molecular weights, optical and epr spectra, total copper contents and different type copper contents of the both proteins were similar. the effects of nan3, kcn, i- and f- on the optical and epr spectra of the proteins were studied. the incubation of ascorbate oxidase with these anions lead to the partial reduction of the copper. the data ob ...	1977	588636
a lectin from the exudate of the fruit of the vegetable marrow (cucurbita pepo) that has a specificity for beta-1,4-linked n-acetylglucosamine oligosaccharides.	lectins are present in the exudate (presumably from the phloem) of the fruits of three species of the cucurbitaceae, namely vegetable marrow (cucurbita pepo), melon (cucumis melo) and cucumber (cucumis sativus). they are all strongly inhibited in their activities by chitin oligosaccharides, but only weakly by n-acetylglucosamine. glycopeptides from soya-bean agglutinin and fetuin are also strong inhibitors of cucurbita pepo lectin, indicating that it interacts with internal n-acetylglucosamine r ...	1979	534476
albumins, glyoxysomal enzymes and globulins in dry seeds of cucumis sativus: qualitative and quantitative analysis.	1) albumins and globulins were prepared from dry seeds of cucumbers (cucumis sativus) by differential extraction. the globulin fraction was analyzed by gel electrophoresis under denaturing conditions in the presence and absence of mercaptoethanol. the subunit (mr = 54000) of the tetramer (mr = 240000) was shown to be composed of two different peptides. microheterogeneity rendered the exact interpretation of the analysis difficult. 2) glyoxysomal proteins were already present in dry seeds: malate ...	1978	422126
plant plasma membrane. correlation between glucan synthase ii activity and a mannosyl transferase activity.	in cotyledons of germinating cucumber seeds (cucumis sativus), plasma membranes were investigated biochemically and partially characterized. glucan synthease ii was utilized as a marker to locate plasma membrane vesicles within fractions obtained by differential centrifugation or within sucrose gradients used either in zonal centrifugations or in sedimentations to equilibrium density. thorough homogenization led to a homogeneous population of plasma membrane vesicles which could be clearly separ ...	1979	116951
plant microbody proteins. purification and glycoprotein nature of glyoxysomal isocitrate lyase from cucumber cotyledons.	1. isocitrate lyase from cotyledons of cucumber seedlings (cucumis sativus) has been purified 100-fold. two methods of preparing the soluble glyoxylate cycle enzyme are described: an elaborated method which used crude extracts of cucumber cotyledons, and another procedure which started with purified glyoxysomes from 4-day-old cotyledons and included a separation of glyoxysomal matrix enzymes by zonal centrifugation. the product behaved as a single species when tested by (a) polyacrylamide gel el ...	1978	103713
influence of nitrogen supply on host and multiplication of cucumber mosaic virus.	the effect of several concentrations of nitrogen (0, 21, 70, 210, 420 and 630 ppm) on the growth and multiplication of cucumber mosaic virus in cucumber (cucumis sativus l. barshati) grown in sand culture was studied. the effect of nitrogen nutrition on the concentration of virus appeared to be directly related to vegetative growth of the host. 420 ppm level yielding the best growth also showed the highest virus concentration and total nitrogen percentage at 30th day of infection.	1977	67769
effect of acid and salt concentration in fresh-pack pickles on the growth of clostridium botulinum spores.	the addition of various amounts of acetic acid to pureed cucumbers inoculated with clostridium botulinum spores has shown that outgrowth is inhibited at ph 4.8 but not at ph 5.0. inoculation experiments with whole cucumbers showed that as little as 0.9% acetic acid in the brine was sufficient to prevent outgrowth from spore inocula as high as 10(6)/cucumber. it was further shown that the rapid rate of acetic acid penetration into fresh-pack pickles prevents the growth of any c. botulinum spores ...	1976	9898
the formation of cis-3-nonenal, trans-2-nonenal and hexanal from linoleic acid hydroperoxide isomers by a hydroperoxide cleavage enzyme system in cucumber (cucumis sativus) fruits.	1. a particulate enzyme fraction and an acetone powder preparation from cucumber fruits cleaved 9- and 13-hydroperoxyoctadecadienoic acids to form volatile aldehydes and oxoacid fragments. 2. from the 9-hydroperoxide, the major volatile fragments were cis-3-nonenal and trans-2-nonenal using particulate enzyme and acetone powder preparations, respectively. 3. hexanal was the only significant volatile fragment from the 13-hydroperoxide. 4. the particulate enzyme system was equally effective on bot ...	1976	8126
some changes in the composition of the fruit of the glasshouse cucumber (cucumis sativus) during growth, maturation and senescence.		1976	5628
p-hydroxybenzoate synthase: a complex associated with mitochondrial membranes of roots of cucumis sativus.		1975	5305
isolation and characterization of indole-3-acetaldehyde reductases from cucumis sativus.	in a continuing study of the biosynthetic pathway and regulatory mechanisms governing indole-3-acetic acid (auxin) formation, we report the isolation and initial characterization of three distinct indole-3-acetaldehyde reductases from cucumber seedlings. these enzymes catalyze the reduction of indole-3-acetaldehyde to indole-3-ethanol with the concomitant oxidation of nad(p)h to nad(p)+. two of the reductases are specific for nadph as second substrate, while the third is specific for nadh. the e ...	1976	2607