Publications

alkylated benzothiophene desulfurization by rhodococcus sp. strain t09.	a benzothiophene desulfurizing bacterium was isolated and identified as rhodococcus sp. strain t09. growth assays revealed that this strain assimilated, as the sole sulfur source, various organosulfur compounds that cannot be assimilated by the well-studied dibenzothiophene-desulfurizing rhodococcus sp. igts8. the cellular growth rate of strain t09 for the alkylated benzothiophenes depended on the alkylated position and the length of the alkyl moiety.	2000	10803960
evidence for diverse oxidations in the catabolism of toluene by rhodococcus rhodochrous strain ofs.	rhodococcus rhodochrous strain ofs grew on toluene as a sole source of carbon and energy with a maximum growth rate of 0.011 h(-1). initial reaction products were extracted, derivatized and identified by gc-ms. oxygen consumption studies indicated that ofs grown on an aliphatic substrate required an induction period before oxidizing toluene. ofs grown on toluene transformed an array of aromatic ground water pollutants including styrene, ethylbenzene and chlorobenzene. products of these transform ...	2000	10803902
resistance of rhodococcus equi to acid ph.	rhodococcus equi is an important gram-positive intracellular facultative pathogen in foals of less than 3 months of age, that causes suppurative bronchopneumonia, lymphadenitis and/or enteritis. the disease in young foals mainly occurs in spring and summer when weather conditions are favorable for survival and multiplication of the bacteria in the environment. r. equi is widespread in the environment of horsebreeding farms: it has been isolated from the soil of paddocks and from the feces of adu ...	2000	10791761
identification of fluoropyrogallols as new intermediates in biotransformation of monofluorophenols in rhodococcus opacus 1cp.	the transformation of monofluorophenols by whole cells of rhodococcus opacus 1cp was investigated, with special emphasis on the nature of hydroxylated intermediates formed. thin-layer chromatography, mass spectrum analysis, and (19)f nuclear magnetic resonance demonstrated the formation of fluorocatechol and trihydroxyfluorobenzene derivatives from each of three monofluorophenols. the (19)f chemical shifts and proton-coupled splitting patterns of the fluorine resonances of the trihydroxyfluorobe ...	2000	10788394
targeted disruption of the kstd gene encoding a 3-ketosteroid delta(1)-dehydrogenase isoenzyme of rhodococcus erythropolis strain sq1.	microbial phytosterol degradation is accompanied by the formation of steroid pathway intermediates, which are potential precursors in the synthesis of bioactive steroids. degradation of these steroid intermediates is initiated by delta(1)-dehydrogenation of the steroid ring structure. characterization of a 2.9-kb dna fragment of rhodococcus erythropolis sq1 revealed an open reading frame (kstd) showing similarity with known 3-ketosteroid delta(1)-dehydrogenase genes. heterologous expression of k ...	2000	10788377
nicotinoprotein (nadh-containing) alcohol dehydrogenase from rhodococcus erythropolis dsm 1069: an efficient catalyst for coenzyme-independent oxidation of a broad spectrum of alcohols and the interconversion of alcohols and aldehydes.	extracts from benzyl-alcohol-grown rhodococcus erythropolis dsm 1069 showed nad(p)-independent, n,n-dimethyl-4-nitrosoaniline (ndma)-dependent alcohol dehydrogenase activity. the enzyme exhibiting this activity was purified to homogeneity and characterized. it appears to be a typical nicotinoprotein as it contains tightly bound nadh acting as cofactor instead of coenzyme. other characteristics indicate that it is highly similar to the known nicotinoprotein alcohol dehydrogenase (np-adh) from amy ...	2000	10784035
haloalkane-utilizing rhodococcus strains isolated from geographically distinct locations possess a highly conserved gene cluster encoding haloalkane catabolism.	the sequences of the 16s rrna and haloalkane dehalogenase (dhaa) genes of five gram-positive haloalkane-utilizing bacteria isolated from contaminated sites in europe, japan, and the united states and of the archetypal haloalkane-degrading bacterium rhodococcus sp. strain ncimb13064 were compared. the 16s rrna gene sequences showed less than 1% sequence divergence, and all haloalkane degraders clearly belonged to the genus rhodococcus. all strains shared a completely conserved dhaa gene, suggesti ...	2000	10781539
effects of non-ionic surfactants on the uptake and hydrolysis of fluoresceindiacetate by alkane-oxidizing bacteria.	biological effects of non-ionic surfactants on alkane-oxidizing bacteria were studied by assessing their influence on the uptake of prefluorochrome fluoresceindiacetate (fda) and its intracellular hydrolysis to fluorescein. both decreasing and increasing rates of hydrolysis as a consequence of the presence of surfactants were observed. the surfactants influenced the uptake of fda, but not its intracellular hydrolysis. the effects of the surfactants on the uptake rate depended strongly on the str ...	2000	10779877
[the degree of halophily in rhodococcus erythropolis and halobacterium salinarum depends on the partial pressure of oxygen].		2000	10776634
mics of oxazolidinones for rhodococcus equi strains isolated from humans and animals.	eperezolid and linezolid are representatives of a new class of orally active, synthetic antimicrobial agents. the in vitro activity values (mics) of linezolid, eperezolid, and comparator antibiotics against 102 strains of rhodococcus equi isolated from humans and animals were determined. linezolid was more active than eperezolid against the strains tested; premafloxacin was the most active comparator antibiotic.	2000	10770781
pulmonary infection in human immunodeficiency virus-infected patients in taiwan.	pulmonary infection is a major cause of morbidity and mortality in human immunodeficiency virus (hiv)-infected patients.	2000	10770026
purification and characterization of a baeyer-villiger mono-oxygenase from rhodococcus erythropolis dcl14 involved in three different monocyclic monoterpene degradation pathways.	a baeyer-villiger mono-oxygenase (bvmo), catalysing the nadph- and oxygen-dependent oxidation of the monocyclic monoterpene ketones 1-hydroxy-2-oxolimonene, dihydrocarvone and menthone, was purified to homogeneity from rhodococcus erythropolis dcl14. monocyclic monoterpene ketone mono-oxygenase (mmkmo) is a monomeric enzyme of molecular mass 60 kda. it contains 1 mol of fad/monomer as the prosthetic group. the n-terminal amino acid sequence showed homology with many other nadph-dependent and fad ...	2000	10769172
synthesis and antifungal activity of rhodopeptin analogues. 2. modification of the west amino acid moiety.	structure-activity relationships of the west amino acid modified analogues of rhodopeptins, novel antifungal tetrapeptide isolated from rhodococcus species mer-n1033, have been investigated. among the analogues synthesized, 2,2-difluoro and 2-hydroxy derivatives retained the antifungal activity with better physical properties, i.e., solubility or acute toxicity.	2000	10768201
synthesis and antifungal activity of rhodopeptin analogues. 1. modification of the east and south amino acid moieties.	structure-activity relationships of the east and south amino acid modified analogues of rhodopeptins, novel antifungal cyclic tetrapeptides isolated from rhodococcus species mer-n1033, have been investigated. it was observed that a basic amino acid moiety (lysine or ornithine) as the east amino acid and a hydrophobic and bulky neutral amino acid (i.e., gamma-methylleucine) as the south amino acid were indispensable structure motifs for antifungal activity of rhodopeptin analogues.	2000	10768200
heterologous expression of bacterial epoxyalkane:coenzyme m transferase and inducible coenzyme m biosynthesis in xanthobacter strain py2 and rhodococcus rhodochrous b276.	coenzyme m (com) (2-mercaptoethanesulfonic acid) biosynthesis is shown to be coordinately regulated with the expression of the enzymes of alkene and epoxide metabolism in the propylene-oxidizing bacteria xanthobacter strain py2 and rhodococcus rhodochrous strain b276. these results provide the first evidence for the involvement of com in propylene metabolism by r. rhodochrous and demonstrate for the first time the inducible nature of eubacterial com biosynthesis.	2000	10762269
characterization of the naphthalene-degrading bacterium, rhodococcus opacus m213.	bacterial strain m213 was isolated from a fuel oil-contaminated soil in idaho, usa, by growth on naphthalene as a sole source of carbon, and was identified as rhodococcus opacus m213 by 16s rdna sequence analysis and growth on substrates characteristic of this species. m213 was screened for growth on a variety of aromatic hydrocarbons, and growth was observed only on simple 1 and 2 ring compounds. no growth or poor growth was observed with chlorinated aromatic compounds such as 2,4-dichloropheno ...	2000	10754253
[storage of industrial microorganisms entrapped into polymer matrices].	our study of the techniques of long-term storage of the biomass of various strains of microorganisms, which cause breakdown or transformation of synthetic organic compounds, demonstrates that desiccated agar beads with immobilized microbial cells can be used for this purpose. in addition, the cells can be stored in desiccated matrices of agar or polyvinyl alcohol, coating synthetic cords. such dry biocatalysts may be used for quick starting of bioreactors and in other biotechnological processes. ...	2000	10752086
[adaptation of acrylamide producer rhodococcus rhodochrous m8 to change in ammonium concentration in medium].	the mechanism of adaptation of the acrylamide producing strain rhodococcus rhodochrous m8 to changes in ammonium concentrations in the medium was studied. an increase in the content of ammonium in the medium changed the activity of glutamine synthetase (gs) (ec 6.3.1.2) and glutamine dehydrogenase (gd) (ec 1.4.1.4), the enzymes of ammonium assimilation, as well as the activities of enzymes responsible for nitrile utilization: nitrile hydratase (ec 4.2.1.84) and amidase (ec 3.5.1.4). this also ca ...	2000	10752079
successful treatment of rhodococcus equi pulmonary infection in a renal transplant recipient.	the rhodococcus is a mycobacterium-like organism which is normally a pathogen in foals. it usually spreads by direct contact or by aerosol from horse faeces and causes pyogranulomatous pulmonary infections. occasionally, it acts opportunistically to infect immuno-compromised human hosts, most commonly those with the acquired immune deficiency syndrome (aids). here we report a pulmonary infection by rhodococcus equi in a renal transplant recipient who was successfully treated. the literature on t ...	2000	10743428
nitrile hydratase and amidase from rhodococcus rhodochrous hydrolyze acrylic fibers and granular polyacrylonitriles.	rhodococcus rhodochrous ncimb 11216 produced nitrile hydratase (320 nkat mg of protein(-1)) and amidase activity (38.4 nkat mg of protein(-1)) when grown on a medium containing propionitrile. these enzymes were able to hydrolyze nitrile groups of both granular polyacrylonitriles (pan) and acrylic fibers. nitrile groups of pan40 (molecular mass, 40 kda) and pan190 (molecular mass, 190 kda) were converted into the corresponding carbonic acids to 1.8 and 1.0%, respectively. in contrast, surfacial n ...	2000	10742253
characterization of the protocatechuic acid catabolic gene cluster from streptomyces sp. strain 2065.	protocatechuate 3,4-dioxygenase (ec 1.13.11.3) catalyzes the ring cleavage step in the catabolism of aromatic compounds through the protocatechuate branch of the beta-ketoadipate pathway. a protocatechuate 3,4-dioxygenase was purified from streptomyces sp. strain 2065 grown in p-hydroxybenzoate, and the n-terminal sequences of the beta- and alpha-subunits were obtained. pcr amplification was used for the cloning of the corresponding genes, and dna sequencing of the flanking regions showed that t ...	2000	10742233
mycolic acids from rhodococcus, gordonia, and dietzia.	the mycolic acids from 11 species of rhodococcus, seven species of gordonia, and one species of dietzia were analyzed using capillary gas chromatography and mass spectrometry (glc/ms). all strains tested in this study were divided into three groups according to the degree of double bonds and the average carbon number (av.nc.) of their mycolic acids. the genus gordonia belongs to the first group possessing an av.nc. in the upper 50s and 60s with 0 to 5 double bonds. some rhodococcus species posse ...	2000	10739337
production of isopropyl cis-6-hexadecenoate by regiospecific desaturation of isopropyl palmitate by a double mutant of a rhodococcus strain.	resting cells of a double mutant noted as ksm-mt66, derived from rhodococcus sp. strain ksm-b-3 by uv irradiation, were found to cis-desaturate isopropyl hexadecanoate, yielding isopropyl cis-6-hexadecenoate. addition of sodium glutamate (1.0%), mg so4 (2 mm), and thiamine (2 mm) increased the productivity of the unsaturated product in phosphate buffer. optimal temperature and ph for the reaction were around 26 degrees c and 7, respectively. under the optimized conditions, more than 50 g/l of is ...	2000	10737199
roles of horizontal gene transfer and gene integration in evolution of 1,3-dichloropropene- and 1,2-dibromoethane-degradative pathways.	the haloalkane-degrading bacteria rhodococcus rhodochrous ncimb13064, pseudomonas pavonaceae 170, and mycobacterium sp. strain gp1 share a highly conserved haloalkane dehalogenase gene (dhaa). here, we describe the extent of the conserved dhaa segments in these three phylogenetically distinct bacteria and an analysis of their flanking sequences. the dhaa gene of the 1-chlorobutane-degrading strain ncimb13064 was found to reside within a 1-chlorobutane catabolic gene cluster, which also encodes a ...	2000	10735862
isolation of rare opportunistic pathogens in hungary: case report and short review of the literature. rhodococcus equi.	rhodococcus equi is a well-established pathogen in foal pneumonia and is increasingly recognized as a pathogen in immunocompromised humans. we have isolated a gram-positive coccobacillus from 8 blood samples and lung tissues of a renal transplant patient. colony morphology, growth in lowenstein-jensen medium, 21 biochemical reactions, the characteristic morphological cycle (coccus-rod-coccus) and the camp test established the r. equi diagnosis. histological studies of 2 lung biopsy specimens rev ...	2000	10735185
operon structure and functional analysis of the genes encoding thermophilic desulfurizing enzymes of paenibacillus sp. a11-2.	paenibacillus a11-2 can efficiently cleave two carbon&bond;sulfur bonds in dibenzothiophene (dbt) and alkyl dbts, which are refractory by conventional petroleum hydrodesulfurization, to remove sulfur atom at high temperatures. an 8.7-kb dna fragment containing the genes for the dbt desulfurizing enzymes of a11-2 was cloned in escherichia coli and characterized. heterologous expression analysis of the deletion mutants identified three open reading frames that were required for the desulfurization ...	2000	10733908
complement activation by mycoloyl glycolipids from mycobacterium tuberculosis and rhodococcus ruber.	in this study, we examined complement activation by mycoloyl glycolipids (mgl) such as trehalose-6,6'-dimycolate (tdm), often termed cord factor, and trehalose-6-monomycolate from mycobacterium tuberculosis and rhodococcus ruber, and also examined the effect of complement binding to mgl on phagocytosis by human monocytes. tdm and tmm, but not glucose mycolate, mannose mycolate or fructose mycolate which differ from tmm only in carbohydrate moiety, exhibited complement activation. tdm and tmm of ...	1999	10730082
cloning of an orf with homology to mycobacterium echa1, encoding the enoyl-coa hydratase, in rhodococcus fascians.	an open reading frame encoding a polypeptide of significant homology (55.7% identity) with the enoyl-coa hydratase encoded by the gene echa1 from mycobacterium tuberculosis has been found in the genome of the plant-pathogen bacteria rhodococcus fascians strain nrrl-b-15096. sequence alignments showed that it possesses several conserved blocks common to e. coli, m. tuberculosis and human mitochondria. one of such blocks includes a glutamate residue located at position 149, corresponding to the gl ...	1999	10727085
trehalose 6,6'-dimycolate (cord factor) enhances neovascularization through vascular endothelial growth factor production by neutrophils and macrophages.	trehalose 6,6'-dimycolate (tdm) plays important roles in the development of granulomatous inflammation during infection with mycobacterium spp., rhodococcus spp., etc. to reveal the augmenting effect of tdm on vascular endothelial growth factor (vegf) production and neovascularization, we investigated murine granulomatous tissue air pouches induced by rhodococcus sp. strain 4306 tdm dissolved in freund's incomplete adjuvant (fia), comparing them to pouches treated with fia alone. histologically, ...	2000	10722600
characterization of the gene cluster involved in isoprene metabolism in rhodococcus sp. strain ad45.	the genes involved in isoprene (2-methyl-1,3-butadiene) utilization in rhodococcus sp. strain ad45 were cloned and characterized. sequence analysis of an 8.5-kb dna fragment showed the presence of 10 genes of which 2 encoded enzymes which were previously found to be involved in isoprene degradation: a glutathione s-transferase with activity towards 1,2-epoxy-2-methyl-3-butene (isoi) and a 1-hydroxy-2-glutathionyl-2-methyl-3-butene dehydrogenase (isoh). furthermore, a gene encoding a second gluta ...	2000	10715003
antimicrobial activity of o-carboranylalanine.	functionalized polyhedral carboranes, including amino acid analogs, have unique physicochemical properties and are used as experimental anticancer agents. however, our current knowledge on their effect in nonmammalian biological systems is limited. we investigated the activity spectrum in vitro of o-carboranylalanine (o-cba), considered to be a highly lipophilic analog of phenylalanine, against representative plant pathogenic bacteria and fungi of various taxonomic position. the antibacterial ef ...	1999	10707765
evaluation of mini-vidas rapid test for detection of listeria monocytogenes from production lines of fresh to cold-smoked fish.	this study was conducted to evaluate the efficacy of the mini-vidas listeria monocytogenes (lmo) system (biomérieux vitek, inc., missouri, usa) for detection of l. monocytogenes in environmental and fish samples from three portuguese cold-smoking plants and from their fresh fish suppliers. mini-vidas-lmo is a fully automated system that uses fluorescent elfa (enzyme linked fluorescent assay) technology for detection of listeria monocytogenes antigens in food. it can be a rapid screening method a ...	2000	10699670
gene cloning and nucleotide sequencing and properties of a cocaine esterase from rhodococcus sp. strain mb1.	a strain of rhodococcus designated mb1, which was capable of utilizing cocaine as a sole source of carbon and nitrogen for growth, was isolated from rhizosphere soil of the tropane alkaloid-producing plant erythroxylum coca. a cocaine esterase was found to initiate degradation of cocaine, which was hydrolyzed to ecgonine methyl ester and benzoate; both of these esterolytic products were further metabolized by rhodococcus sp. strain mb1. the structural gene encoding a cocaine esterase, designated ...	2000	10698749
[conjugative transfer of a plasmid from escherichia coli to various strains of the order actinomycetales].	the conjugal transfer of autonomous and integrative plasmids from the donor strain escherichia coli s17-1 to strains of genera actinomadura, arthrobacter, kitasatoa, micromonospora, nocardia, rhodococcus, saccharopolyspora, and to 16 strains of the genus streptomyces was demonstrated. the status of plasmids in recipient strains and the stability of their inheritance were analyzed. plasmids constructed for strains of the genus streptomyces were shown to function in a large number of strains belon ...	1999	10687092
the rhodococcus fascians-plant interaction: morphological traits and biotechnological applications.	rhodococcus fascians is a gram-positive bacterium that infects dicotyledonous and monocotyledonous plants, leading to an alteration in the normal growth process of the host. the disease results from the modulation of the plant hormone balances, and cytokinins are thought to play an important role in the induction of symptoms. generally, on the aerial parts of the plants, existing meristems were found to be most sensitive to the action of r. fascians, but, depending on the infection procedure, di ...	2000	10664130
characterization of the 20s proteasome from the actinomycete frankia.	frankia is an actinomycete that fixes atmospheric nitrogen in symbiotic association with the root systems of a variety of non-leguminous plants, denominated actinorhizal plants. information on the biology of proteolysis in frankia is almost non-existent as it is extremely difficult to grow this organism. we have purified 20s proteasomes from frankia strain acn14a/ts-r. it is composed of one alpha-subunit and one beta-subunit, which assemble into the canonical structure of four rings of seven sub ...	2000	10652097
[bacteremic pneumonia due to rhodococcus equi in a patient with human immunodeficiency virus infection and visceral leishmaniasis].		1999	10650653
[pulmonary infection from rhodococcus equi after renal transplantation. review of the literature].	rhodococcus equi, a strictly aerobic gram positive coco-bacillus, is a pathogen for horses and foals. it may induce opportunistic infections and is described in aids infected patients. we report the case of a 47-year old man, breeder of horses, with kidney transplant who has presented, 8 years after his graft, an impairment of health, a fever and evidence of pulmonary disease. the pulmonary biopsy under scanner guidance and microbiology study, has displayed the diagnosis of rhodococcus equi infe ...	1999	10642987
humus bacteria of norway spruce stands: plant growth promoting properties and birch, red fescue and alder colonizing capacity.	we studied the potential of the humus layer of the norway spruce stands to supply beneficial rhizobacteria to birch (betula pendula), alder (alnus incana) and fescue grass (festuca rubra), representatives of pioneer vegetation after clear-cutting of the coniferous forest. axenically grown seedlings of these species were inoculated with the acid spruce humus, ph 3.7-5.3. actinorhizal propagules, capable of nodulating alder, were present in high density (10(3) g(-1)) in humus of long-term limed pl ...	2000	10640667
biochemical identification and biophysical characterization of a channel-forming protein from rhodococcus erythropolis.	organic solvent extracts of whole cells of the gram-positive bacterium rhodococcus erythropolis contain a channel-forming protein. it was identified by lipid bilayer experiments and purified to homogeneity by preparative sodium dodecyl sulfate (sds)-polyacrylamide gel electrophoresis (page). the pure protein had a rather low molecular mass of about 8.4 kda, as judged by sds-page. sds-resistant oligomers with a molecular mass of 67 kda were also observed, suggesting that the channel is formed by ...	2000	10633112
cobalt-substituted fe-type nitrile hydratase of rhodococcus sp. n-771.	when the genes encoding alpha and beta subunits of fe-type nitrile hydratase (nhase) from rhodococcus sp. n-771 were expressed in escherichia coli in co-supplemented medium without co-expression of the nhase activator, the nhase specifically incorporated not fe but co ion into the catalytic center. the produced co-substituted enzyme exhibited rather weak nhase activity, initially. however, the activity gradually increased by the incubation with an oxidizing agent, potassium hexacyanoferrate. the ...	2000	10631329
cloning and characterization of a novel cis-naphthalene dihydrodiol dehydrogenase gene (narb) from rhodococcus sp. ncimb12038.	rhodococcus sp. ncimb112038 can utilize naphthalene as its sole carbon and energy source. the gene encoding cis-naphthalene dihydrodiol dehydrogenase (narb) of this strain has been cloned and sequenced. expression of ncimb12038 cis-naphthalene dihydrodiol dehydrogenase was demonstrated in escherichia coli cells. narb encodes a putative protein of 271 amino acids and shares 39% amino acid identity with the cis-naphthalene dihydrodiol dehydrogenase from pseudomonas putida g7. comparison of narb wi ...	2000	10620687
rhodococcus equi nosocomial meningitis cured by levofloxacin and shunt removal.		2000	10619769
analysis of the relative abundance of different types of bacteria capable of toluene degradation in a compost biofilter.	a microbial community of a compost biofilter treating toluene vapors was investigated using serum-bottle assays and mineral-agar plates. toluene was not consumed in the absence of oxygen. however, filter-bed extracts exposed to toluene vapor as the only carbon source produced distinct colony types (phenotypic groups) that were counted separately. strains from each group were isolated and checked for toluene-degradation activity in serum bottles. only 15% of colonies were true toluene degraders. ...	1999	10616721
an extractive membrane biofilm reactor for degradation of 1,3-dichloropropene in industrial waste water.	a bacterial biofilm, capable of mineralising a technical mixture of cis- and trans-1,3-dichloropropene (dcpe), was enriched on the biomedium side of an extractive membrane biofilm reactor (embr). the membrane separates the biomedium from the industrial waste water, in terms of ph, ionic strength and the concentration of toxic chemicals. the biofilm, attached to a silicone membrane, is able to mineralize dcpe after its diffusion through the membrane. five bacterial strains with degradation capabi ...	1999	10616720
characterization of is1676 from rhodococcus erythropolis sq1.	to develop a transposable element-based system for mutagenesis in rhodococcus, we used the sacb gene from bacillus subtilis to isolate a novel transposable element, is1676, from r. erythropolis sq1. this 1693 bp insertion sequence is bounded by imperfect (10 out of 13 bp) inverted repeats and it creates 4 bp direct repeats upon insertion. comparison of multiple insertion sites reveals a preference for the sequence 5'-(c/t)ta(a/g)-3' in the target site. is1676 contains a single, large (1446 bp) o ...	1999	10616714
light inhibits rifampicin inactivation and reduces rifampicin resistance due to a cloned mycobacterial adp-ribosylation gene.	rifampicin is a principal drug used to combat infections by mycobacteria and related organisms. most strains of mycobacterium are able to inactivate this antibiotic by ribosylation via an adp-ribosylated intermediate. we found that this inactivation was inhibited by light at levels similar to those prevailing in laboratory environments. rifampicin resistance arising from the cloned adp-ribosyl transferase was also greatly diminished at these light levels. the cloned rhodococcus equi monooxygenas ...	2000	10612740
search for an antibody profile of rhodococcus equi infection in aids patients despite the diversity of isolates and patient immune dysfunction.	diversity of virulence-associated antigens of rhodococcus equi was detected among thirteen strains isolated from aids patients on two continents. one out of four brazilian isolates presented the virulence-associated antigen of 15- to 17-kda, and the other three isolates had the 20-kda virulence-associated antigen. in contrast, only three out of nine italian isolates were positive for virulence-associated antigens - two for the 15- to 17-kda antigen and one for the 20-kda antigen. in four other i ...	1999	10611743
effect of prophylactic administration of hyperimmune plasma to prevent rhodococcus equi infection on foals from endemically affected farms.	the effect on foals of prophylactic administration of hyperimmune plasma to prevent r. equi infection was investigated on three farms at which r. equi infection was endemic. sixteen foals between 10 and 39 days of age were intravenously given 1-21 of hyperimmune plasma. elisa antibody titres against r. equi were significantly increased and maintained at high levels for over 30 days in most of the recipient foals. the prevalence of r. equi infection was 6.3% (1/16) in the foals that received the ...	1999	10605374
a pentacoordinated di-n-carboxamido-dithiolato-o-sulfinato-iron(iii) complex related to the metal site of nitrile hydratase.	postcoordination oxidation by dioxygen of one of the thiolate groups in a pentadentate n(2)s(3) ligand results in an iron(iii) complex with two n-carboxamido, two thiolato, and one o-sulfinato ligands (see the cameron representation). this novel mixed coordination is similar to that determined for the inactive form of the nitrile hydratase from rhodococcus sp. n-771, but differs by the o versus s binding of the sulfinato ligand.	1999	10602230
nitrilase of rhodococcus rhodochrous j1. conversion into the active form by subunit association.	nitrilase-containing resting cells of rhodococcus rhodochrous j1 converted acrylonitrile and benzonitrile to the corresponding acids, but the purified nitrilase hydrolyzed only benzonitrile, and not acrylonitrile. the activity of the purified enzyme towards acrylonitrile was recovered by preincubation with 10 mm benzonitrile, but not by preincubation with aliphatic nitriles such as acrylonitrile. it was shown by light-scattering experiments, that preincubation with benzonitrile led to the assemb ...	2000	10601860
mediastinitis due to gordona sputi after cabg.	genus gordona is included in mycolic acid containing bacteria. this genus infection is very rare and occurs classically in immuno-compromised patients. we report a patient who developed mediastinitis due to gordona sputi after coronary artery bypass grafting (cabg) using left internal mammary artery. immunocompromised factors were not noticed in this case but postoperative bleeding, the most important risk factor of mediastinitis, was found in his course. the treatment was antibiotic therapy, su ...	1999	10597001
haloalkane dehalogenases: structure of a rhodococcus enzyme.	the hydrolytic haloalkane dehalogenases are promising bioremediation and biocatalytic agents. two general classes of dehalogenases have been reported from xanthobacter and rhodococcus. while these enzymes share 30% amino acid sequence identity, they have significantly different substrate specificities and halide-binding properties. we report the 1.5 a resolution crystal structure of the rhodococcus dehalogenase at ph 5.5, ph 7.0, and ph 5.5 in the presence of nai. the rhodococcus and xanthobacte ...	1999	10587433
nad(+)-dependent (s)-specific secondary alcohol dehydrogenase involved in stereoinversion of 3-pentyn-2-ol catalyzed by nocardia fusca aku 2123.	an nad(+)-dependent alcohol dehydrogenase was purified to homogeneity from nocardia fusca aku 2123. the enzyme catalyzed (s)-specific oxidation of 3-pentyn-2-ol (pyoh), i.e., part of the stereoinversion reaction for the production of (r)-pyoh, which is a valuable chiral building block for pharmaceuticals, from the racemate. the enzyme used a broad variety of secondary alcohols including alkyl alcohols, alkenyl alcohols, acetylenic alcohols, and aromatic alcohols as substrates. the oxidation was ...	1999	10586501
analysis of the reaction mechanism and substrate specificity of haloalkane dehalogenases by sequential and structural comparisons.	haloalkane dehalogenases catalyse environmentally important dehalogenation reactions. these microbial enzymes represent objects of interest for protein engineering studies, attempting to improve their catalytic efficiency or broaden their substrate specificity towards environmental pollutants. this paper presents the results of a comparative study of haloalkane dehalogenases originating from different organisms. protein sequences and the models of tertiary structures of haloalkane dehalogenases ...	1999	10585505
regiospecific internal desaturation of aliphatic compounds by a mutant rhodococcus strain.	a mutant rhodococcus strain lacking the ability to utilize 1-chlorohexadecane was found to cis-desaturate aliphatic compounds, such as 1-chlorohexadecane, n-hexadecane, and heptadecanonitrile, yielding corresponding products with a double bond mainly at the ninth carbon from the terminal methyl groups. a new oxidative pathway involving the cis-desaturation step was suggested for alkane utilization by rhodococcus spp.	1999	10584034
identification and environmental detection of rhodococcus species by 16s rdna-targeted pcr.	bacteria of the genus rhodococcus can degrade a wide range of organic pollutants and catalyse many useful biotransformations. there is a need for improved tests to identify rhodococcus species. pcr-based methods for species identification offer advantages in terms of speed and accuracy over traditional methods and can allow direct detection of microbes in environmental samples., pcr tests, using primers targeted at species-specific sequences in the 16s rrna gene, were successfully developed for ...	1999	10583674
bacterial degradation of hydrocarbons as evidenced by respirometric analysis.	the microbial biodegradability of mineral oil and other hydrocarbons, namely hexane, decane and tetradecane was determined using the warburg constant volume respirometer. results of oxygen uptake indicated that hexane and tetradecane were more degradable than mineral oil and decane. rhodococcus erythropolis and erwinia cancerogena showed the highest (0.866) and lowest (0.115) oxygen quotient (qo2) values, respectively, when exposed to mineral oil. staphylococcus warneri and enterobacter cloacae ...	1999	10582377
rhodopeptins, novel cyclic tetrapeptides with antifungal activities from rhodococcus sp. iii. synthetic study of rhodopeptins.	total syntheses of cyclo (-gly-l-lys-l-val-(r)-3-aminododecanoyl-); lv9na and its diastereomer cyclo (-gly-l-lys-l-val-(s)-3-aminododecanoyl-); lv9nb, congeners of rhodopeptin b5 on beta-amino acid moiety, were achieved. the beta-amino acid moiety was prepared as a racemate by the thermal michael addition of an amine to alpha,beta-unsaturated ester. the racemic beta-amino acids were converted to their l-valylamide derivatives and the obtained diastereomers were separated. coupling of both diaste ...	1999	10580384
rhodopeptins, novel cyclic tetrapeptides with antifungal activities from rhodococcus sp. ii. structure elucidation.	the structures of rhodopeptins, novel antifungal peptides, were determined on the basis of physico-chemical analyses of the intact molecules and their acid hydrolysates. the structures of rhodopeptins c1, c2, c3, c4 and b5 were determined to be cyclo (-gly-l-orn-l-val-3-amino-10-methyldodecanoyl-), cyclo (-gly-l-orn-l-ile-3-amino-10-methyldodecanoyl-), cyclo (-gly-l-orn-l-val-3-amino-12-methyltridecanoyl-), cyclo (-gly-l-orn-l-val-3-amino- 12-methyltetradecanoyl-) and cyclo (-gly-l-lys-l-val-3-a ...	1999	10580383
rhodopeptins (mer-n1033), novel cyclic tetrapeptides with antifungal activity from rhodococcus sp. i. taxonomy, fermentation, isolation, physico-chemical properties and biological activities.	five novel cyclic tetrapeptides, named rhodopeptin c1, c2, c3, c4 and b5, were isolated from a strain named rhodococcus sp. mer-n1033. they are a novel type of cyclic tetrapeptide composed of a beta-amino acid and three usual alpha-amino acids. rhodopeptins show high in vitro antifungal activity against candida albicans and cryptococcus neoformans, whereas they show no activity against bacteria.	1999	10580382
in vitro activity of moxifloxacin, a new 8-methoxyquinolone, against gram-positive bacteria.	the in vitro activity of moxifloxacin, formerly bay 12-8039, against gram-positive bacteria was tested by the agar dilution method. a total of 189 isolates that included staphylococcus aureus, enterococcus faecalis, enterococcus faecium, streptococci, rhodococci, leuconostocs, pediococci, lactobacilli, and diphtheroids were tested. moxifloxacin showed greater potency than ciprofloxacin against s. aureus, streptococci, and enterococci, having minimal inhibitory concentrations (mics) lower than th ...	1999	10579093
the structure of the specific capsular polysaccharide of rhodococcus equi serotype 4.	the specific capsular polysaccharide produced by rhodococcus equi serotype 4 was found to be a high-molecular-weight acidic polymer composed of d-glucose, d-mannose, pyruvic acid and a previously unidentified 5-amino-3,5-dideoxynonulosonic (rhodaminic) acid in the proportions 2:1:1:1. structural analysis, employing a combination of microanalytical methods, nuclear magnetic resonance spectroscopy, and mass spectrometric techniques, established that the polysaccharide consisted of linear repeating ...	1999	10573859
characterization of activity and expression of isocitrate lyase in mycobacterium avium and mycobacterium tuberculosis.	analysis by two-dimensional gel electrophoresis revealed that mycobacterium avium expresses several proteins unique to an intracellular infection. one abundant protein with an apparent molecular mass of 50 kda was isolated, and the n-terminal sequence was determined. it matches a sequence in the m. tuberculosis database (sanger) with similarity to the enzyme isocitrate lyase of both corynebacterium glutamicum and rhodococcus fascians. only marginal similarity was observed between this open readi ...	1999	10572116
establishment of a gene transfer system for rhodococcus opacus pd630 based on electroporation and its application for recombinant biosynthesis of poly(3-hydroxyalkanoic acids).	a gene transfer system for rhodococcus opacus pd630 based on electroporation was established and optimized employing the escherichia coli-rhodococcus shuttle vectors pnc9501 and pnc9503 as well as the e. coli-corynebacterium glutamicum shuttle vector pjc1 as suitable cloning vectors for r. opacus pd630, resulting in transformation efficiencies up to 1.5 x 10(5) cfus/microgram plasmid dna. applying the optimized electroporation protocol to the pnc9501-derivatives pak68 and pak71 harboring the ent ...	1999	10570798
alpha5 subunit in trypanosoma brucei proteasome can self-assemble to form a cylinder of four stacked heptamer rings.	the proteasomes have a central role in catalysing protein degradation among both prokaryotes and eukaryotes. the 20 s proteasome constitutes their catalytic core. in studying the structure of trypanosoma brucei 20 s proteasomes, we isolated by two-dimensional (2d) gel electrophoresis a 27 kda subunit protein with an estimated pi of 4.7 and subjected it to mass spectrometric analysis. a tryptic peptide sequence from the protein was found identical with that of the rat alpha5 subunit. with the use ...	1999	10567215
sequence analysis of the oxidase/reductase genes upstream of the rhodococcus erythropolis aldehyde dehydrogenase gene thca reveals a gene organisation different from mycobacterium tuberculosis.	the sequence of the dna region upstream of the thiocarbamate-inducible aldehyde dehydrogenase gene thca of rhodococcus erythropolis ni86/21 was determined. most of the predicted orfs are related to various oxidases/reductases, including short-chain oxidases/reductases, gmc oxidoreductases, alpha-hydroxy acid oxidases (subfamily 1 flavin oxidases/dehydrogenases), and subfamily 2 flavin oxidases/dehydrogenases. one orf is related to enzymes involved in biosynthesis of pqq or molybdopterin cofactor ...	1999	10565547
preferential oxidative dehalogenation upon conversion of 2-halophenols by rhodococcus opacus 1g.	the regiospecificity of hydroxylation of c2-halogenated phenols by rhodococcus opacus 1g was investigated. oxidative defluorination at the c2 position ortho with respect to the hydroxyl moiety was preferred over hydroxylation at the non-fluorinated c6 position for all 2-fluorophenol compounds studied. initial hydroxylation of 2,3, 5-trichlorophenol resulted in the exclusive formation of 3, 5-dichlorocatechol. these results indicate that, in contrast to all other phenol ortho-hydroxylases studied ...	1999	10564791
otomastoiditis caused by rhodococcus equi in a patient with aids.	rhodococcus equi is a well-recognized pathogen in veterinary medicine and a rare but well-documented cause of cavitary pneumonia in immunocompromised patients. most cases of rhodococcus equi infections in these patients involve the lungs. otomastoiditis due to rhodococcus equi is rare, and disseminated rhodococcus equi with otomastoiditis has never been reported. we report a case of otomastoiditis with systemic dissemination due to rhodococcus equi in a patient with aids.	1999	10547875
microbial desulfurization of alkylated dibenzothiophenes from a hydrodesulfurized middle distillate by rhodococcus erythropolis i-19.	rhodococcus erythropolis i-19, containing multiple copies of key dsz genes, was used to desulfurize alkylated dibenzothiophenes (cx-dbts) found in a hydrodesulfurized middle-distillate petroleum (md 1850). initial desulfurization rates of dibenzothiophene (dbt) and md 1850 by i-19 were 5.0 and 2.5 micromol g dry cell weight(-1) min(-1), more than 25-fold higher than that for wild-type bacteria. according to sulfur k-edge x-ray absorption near-edge structure (xanes) analysis, thiophenic compounds ...	1999	10543810
evaluation of macromolecular electron-density map quality using the correlation of local r.m.s. density.	it has recently been shown that the standard deviation of local r.m. s. electron density is a good indicator of the presence of distinct regions of solvent and protein in macromolecular electron-density maps [terwilliger & berendzen (1999). acta cryst. d55, 501-505]. here, it is demonstrated that a complementary measure, the correlation of local r.m.s. density in adjacent regions on the unit cell, is also a good measure of the presence of distinct solvent and protein regions. the correlation of ...	1999	10531485
reciprocal-space solvent flattening.	solvent flattening is a powerful tool for improving crystallographic phases for macromolecular structures obtained at moderate resolution, but uncertainties in the optimal weighting of experimental phases and modified phases make it difficult to extract all the phase information possible. solvent flattening is essentially an iterative method for maximizing a likelihood function which consists of (i) experimental phase information and (ii) information on the likelihood of various arrangements of ...	1999	10531484
changing epidemiology of infections in patients with neutropenia and cancer: emphasis on gram-positive and resistant bacteria.	over the past 3 decades, considerable changes have occurred in the types of bacteria causing infection in febrile patients with neutropenia and cancer. twenty years ago, gram-negative bacteria caused approximately 70% of bloodstream infections. as a probable consequence of long-dwelling intravascular devices, fluoroquinolone prophylaxis, and high-dose chemotherapy-induced mucositis, there has been a shift toward gram-positive coccal bacteremia. in most centers today, approximately 70% of bactere ...	1999	10530434
relationships between demethylase activity, formaldehyde and oxygen during incubation of rhodococcus erythropolis with veratrate.	relationships between demethylase activity, formaldehyde and oxygen were investigated. demethylase activity was measured against the following substrates: veratric, vanillic, and isovanillic acids, as well as in the presence of guaiacol. the influence of atp and gtp on demethylase activity was also checked. demethylase activity was found to be dependent on the capability of the cells for endogenous oxygen uptake. in some cases atp produced the opposite effect: instead of being taken up, oxygen w ...	1998	10526986
severe otitis and mastoiditis due to rhodococcus equi in a patient with aids. case report.	we report a case of otitis media associated with pneumonia due to rhodococcus equi. a 31-year-old patient with aids presented with cough and right facial palsy. imaging revealed right otitis media and severe temporal bone destruction, associated with pneumonia. r. equi was isolated from ear secretions, blood, and sputum. the radiologic findings are described. this unusual pathogen should be included in the differential diagnosis of the immunocompromised patient with aggressive otitis.	1999	10525774
bacteremia due to dietzia maris in an immunocompromised patient.		1999	10524995
production and partial characterization of antibody to cord factor (trehalose 6,6'-dimycolate) in mice.	antibody production against the trehalose 6,6'-dimycolate (tdm, cord factor) of rhodococcus ruber, a non-pathogenic species of the actinomycetales group, was investigated in mice by repeated intraperitoneal injection of tdm in water-in-oil-in-water micelles without carrier protein. the antigenic tdm was isolated and purified chromatographically from the chloroform-methanol extractable lipids of r. ruber. the hydrophobic moiety of this tdm was composed of two molecules of monoenoic or dienoic alp ...	1999	10524797
utilization of trihalogenated propanes by agrobacterium radiobacter ad1 through heterologous expression of the haloalkane dehalogenase from rhodococcus sp. strain m15-3.	trihalogenated propanes are toxic and recalcitrant organic compounds. attempts to obtain pure bacterial cultures able to use these compounds as sole carbon and energy sources were unsuccessful. both the haloalkane dehalogenase from xanthobacter autotrophicus gj10 (dhla) and that from rhodococcus sp. strain m15-3 (dhaa) were found to dehalogenate trihalopropanes to 2,3-dihalogenated propanols, but the kinetic properties of the latter enzyme are much better. broad-host-range dehalogenase expressio ...	1999	10508091
essential tyrosine residues in 3-ketosteroid-delta(1)-dehydrogenase from rhodococcus rhodochrous.	tetranitromethane treatment of 3-ketosteroid-delta(1)-dehydrogenase of rhodococcus rhodochrous caused loss of the catalytic activity in a time- and concentration-dependent manner. peptides (p-81) and (pn-83) were isolated from tryptic digests of the native and tetranitromethane-treated enzyme proteins, respectively. pn-83 was the nitrated form of p-81. the amino acid sequence was ggaplidylesdddlefmvypwpdyfgk (positions 97-124 of the dehydrogenase sequence). pn-83 showed a low yield of pth-tyr of ...	1999	10502672
purification and characterization of a novel naphthalene dioxygenase from rhodococcus sp. strain ncimb12038.	we report here the characterization of the catalytic component (isp(nar)) of a new naphthalene dioxygenase from rhodococcus sp. strain ncimb12038. the genes encoding the two subunits of isp(nar) are not homologous to their previously characterized counterparts in pseudomonas. the deduced amino acid sequences have only 33 and 29% identity with the corresponding subunits in pseudomonas putida ncib 9816-4, for which the tertiary structure has been reported.	1999	10498739
modulation of cytokine response of pneumonic foals by virulent rhodococcus equi.	the ability of rhodococcus equi to induce pneumonia in foals depends on the presence of an 85- to 90-kb plasmid. in this study, we evaluated whether plasmid-encoded products mediate virulence by modulating the cytokine response of foals. foals infected intrabronchially with a virulence plasmid-containing strain of r. equi had similar gamma interferon (ifn-gamma) and interleukin-12 (il-12) p35 but significantly higher il-1beta, il-10, il-12 p40, and tumor necrosis factor alpha (tnf-alpha) mrna ex ...	1999	10496876
nitrile hydratase from rhodococcus erythropolis: metabolization of steroidal compounds with a nitrile group.	the progestin dienogest (17alpha-cyanomethyl-17beta-hydroxy-estra-4,9-dien-3-one) was metabolized by the nitrile hydratase-containing microorganism rhodococcus erythropolis. an enzymatic hydrolysis of the nitrile group at the 17alpha-side chain was intended to obtain novel derivatives and to test them for progesterone receptor affinity. in contrast to the rapid enzymatic hydrolysis of nonsteroidal nitriles, the nitrile group of dienogest was cleaved very slowly. the dominant reaction was an arom ...	1999	10493599
purification and partial characterization of caffeine oxidase--a novel enzyme from a mixed culture consortium.	cell-free extract prepared from a mixed culture consisting of strains belonging to the genera klebsiella and rhodococcus grown in the presence of caffeine contains a novel enzyme, caffeine (1,3, 7-trimethylxanthine) oxidase which catalyzes the oxidation of caffeine at the c-8 position to produce 1,3,7-trimethyluric acid. the enzyme was purified to homogeneity by a combination of ion-exchange and hydrophobic column chromatographies. both native and sds/page of the purified enzyme showed a single ...	1999	10491316
tnf receptor p55 is required for elimination of inflammatory cells following control of intracellular pathogens.	the elimination of lymphocytes within inflammatory lesions is a critical component in the resolution of disease once pathogens have been cleared. we report here that signaling through the tnf receptor p55 (tnfrp55) is required to eliminate lymphocytes from lesions associated with intracellular pathogens. thus, tnfrp55-/- mice, but not fas-deficient mice, maintained inflammatory lesions associated with either leishmania major or rhodococcus equi infection, although they developed a th1 response a ...	1999	10490988
restriction fragment length polymorphisms of virulence plasmids in rhodococcus equi.	virulent rhodococcus equi, which is a well-known cause of pyogranulomatous pneumonia in foals, possesses a large plasmid encoding virulence-associated 15- to 17-kda antigens. foal and soil isolates from five countries-argentina, australia, canada, france, and japan-were investigated for the presence of 15- to 17-kda antigens by colony blotting, using the monoclonal antibody 10g5, and the gene coding for 15- to 17-kda antigens by pcr. plasmid dnas extracted from positive isolates were digested wi ...	1999	10488224
[pulmonary malacoplakia caused by rhodococcus equi in aids: a case report].	we describe the observation of a right upper lobe consolidation with cavitation produced by rhodococcus equi in a patient suffering from aids. the inefficacy of a prolonged antimicrobial therapy adapted against r. equi led to a right upper lobectomy. the histopathology showed a pseudotumoral mass, with dense infiltration of macrophages containing michaelis-gutmann bodies, which was positive for the culture of r. equi. pulmonary malacoplakia with rhodococcus equi was diagnosed. this pathology sho ...	1999	10486839
expression of a functional antibody fragment in the gut of rhodnius prolixus via transgenic bacterial symbiont rhodococcus rhodnii.	expression within insects of foreign antiparasitic gene products via microbial symbionts could be used to prevent transmission of vector-borne pathogens to vertebrate hosts. genetically transformed symbiotic bacteria rhodococcus rhodnii expressed functional antibody fragments (rdb3 encoding murine v(h)/k which binds progesterone) that were exported into the gut lumen of the triatomine bug rhodnius prolixus (hemiptera: reduviidae), a vector of chagas disease. transgenic symbionts were maintained ...	1999	10484156
stereoselective carveol dehydrogenase from rhodococcus erythropolis dcl14. a novel nicotinoprotein belonging to the short chain dehydrogenase/reductase superfamily.	a novel nicotinoprotein, catalyzing the dichlorophenolindophenol-dependent oxidation of carveol to carvone, was purified to homogeneity from rhodococcus erythropolis dcl14. the enzyme is specifically induced after growth on limonene and carveol. dichlorophenolindophenol-dependent carveol dehydrogenase (cdh) is a homotetramer of 120 kda with each subunit containing a tightly bound nad(h) molecule. the enzyme is optimally active at ph 5.5 and 50 degrees c and displays a broad substrate specificity ...	1999	10473585
rhodococcus equi infections: clinical features and laboratory diagnosis.		1999	10472487
nitrile hydratase involved in aldoxime metabolism from rhodococcus sp. strain yh3-3 purification and characterization.	nitrile hydratase responsible for aldoxime metabolism from the e-pyridine-3-aldoxime degrading bacterium, rhodococcus sp. strain yh3-3 was purified and characterized. addition of cobalt ion was necessary for the formation of enzyme. the enzyme activity was highly induced not only by nitriles and amides but also by several aldoxime compounds. the enzyme was purified approximately 108-fold with a 16% yield from the cell-free extract of the strain. the native enzyme had a mr of approximately 130 00 ...	1999	10469129
steroid monooxygenase of rhodococcus rhodochrous: sequencing of the genomic dna, and hyperexpression, purification, and characterization of the recombinant enzyme.	steroid monooxygenase of rhodococcus rhodochrous is a baeyer-villigerase catalyzing the insertion of an oxygen atom between the c(17)- and c(20)-carbons of progesterone to produce testosterone acetate. the 5.1-kbp-long bamhi dna fragment containing the steroid monooxygenase gene, smo, was cloned from the chromosomal dna and sequenced. the smo gene is 1,650 nucleotides long, starts with a ttg codon, and ends with a tga codon. the deduced amino acid sequence indicates that the enzyme protein consi ...	1999	10467180
batch culture biodegradation of methylhydrazine contaminated nasa wastewater.	the batch culture degradation of nasa wastewater containing mixtures of citric acid, methylhydrazine, and their reaction product was studied. the organic contaminants present in the nasa wastewater were degraded by achromobacter sp., rhodococcus b30 and rhodococcus j10. while the achromobacter sp. showed a preference for the degradation of the citric acid, the rhodococcus species were most effective in reducing the methylhydrazine and the reaction product. removals of more than 50% were observed ...	1999	10466199
developments in destructive and non-destructive pathways for selective desulfurizations in oil-biorefining processes	biocatalytic desulfurization is still not a commercial technology, but conceptual engineering and sensitivity analyses have shown that the approach is very promising. the purpose of this paper is to investigate further some aspects of the biodesulphurization pathways, discussing the non-destructive pathway with the well-known rhodococcus rhodochrous igts8. findings revealed byproducts, such as 2'-hydroxybiphenyl (hbp), sulfite and sulfate, obtained by the desulfurization of dibenzothiophene (dbt ...	1999	10461376
biosynthesis of polyhydroxyalkanoates from low-rank coal liquefaction products by pseudomonas oleovorans and rhodococcus ruber	a screening identified several bacteria that were able to use chemically heterogeneous low-rank coal liquefaction products as complex carbon sources for growth. pseudomonas oleovorans and rhodococcus ruber accumulated polyhydroxyalkanoic acids (pha) amounting to 2%-8% of the cell dry weight when the cells were cultivated on these liquefaction products in the absence of any other carbon source. r. ruber accumulated, in addition to pha, small amounts of triacylglycerols. the accumulated pha consis ...	1999	10461375
degradation of alicyclic molecules by rhodococcus ruber cd4.	the present work describes investigations on the bacterial degradation of the alicyclic molecule cyclododecane. it represents a structure where the initial degradative steps have to be similar to a "subterminal" attack as there is no "terminal" part of the molecule. we were able to show that the gram-positive bacterium rhodococcus ruber cd4 dsm 44394 oxidizes cyclododecane to the corresponding alcohol and ketone, the latter being subject to ring fission by a baeyer-villiger oxygenase. this key e ...	1999	10461374
requirement for a different hydrophobic moiety and reliable chromogenic substrate for endo-type glycosylceramidases.	a series of synthetic lactosides with aglycones that differed in length and structure were used to determine the substrate specificity of endo-type glycosylceramidases. endoglycoceramidases (egcase) from bacteria preferred lactosides with an acylamide structure over simple n-alkyl lactosides. while ceramide glycanase (cgase) from leech did not show preference. n -acylaminoethyl beta-lactosides and n -alkyl lactosides were substrates for both egcase and cgase, but n-acylaminobutyl beta-lactosides ...	1999	10460837
pulmonary malacoplakia associated with rhodococcus equi infection in patients with aids: case report and review.		1999	10451186
cometabolic oxidation of phenanthrene to phenanthrene trans-9,10-dihydrodiol by mycobacterium strain s1 growing on anthracene in the presence of phenanthrene.	mycobacterium strain s1, originally described as rhodococcus strain s1 by chemotaxonomic criteria, was isolated by growth on anthracene, and is unable to use any of nine other polycyclic aromatic compounds as carbon source. metabolism of phenanthrene during growth on anthracene as sole carbon source results in the accumulation of traces of a dihydrodiol metabolite in the growth medium, which, by comparison with authentic standards, has been tentatively identified as phenanthrene trans-9,10-dihyd ...	1999	10446712
differential detection of key enzymes of polyaromatic-hydrocarbon-degrading bacteria using pcr and gene probes.	bacteria with ability to degrade polyaromatic hydrocarbons (pahs), isolated from wastewater and soil samples, were investigated for their taxonomic, physiological and genetic diversity. eighteen isolates able to metabolize naphthalene or phenanthrene as sole carbon source were taxonomically affiliated to different subclasses of the proteobacteria (sphingomonas spp., acidovorax spp., comamonas spp. and pseudomonas spp.) and to phyla of gram-positive bacteria with low and high dna g + c content (p ...	1999	10439412
degradation of trichloroethene by a linear-plasmid-encoded alkene monooxygenase in rhodococcus corallinus (nocardia corallina) b-276.	rhodococcus corallinus (formerly nocardia corallina) b-276, isolated with propene as sole carbon and energy source, is able to oxidize trichloroethene (tce). glucose- or propene-grown r. corallinus b-276 cells exhibited no difference in tce degradation efficiency. tce degradation was found to be growth-phase-dependent and maximum rates were monitored with stationary-phase cells. k(m) and vmax values for tce degradation of r. corallinus b-276 grown in nutrient broth medium in the presence of gluc ...	1999	10439411