Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| mechanisms of chloride secretion induced by thermostable direct haemolysin of vibrio parahaemolyticus in human colonic tissue and a human intestinal epithelial cell line. | thermostable direct haemolysin (tdh) produced by vibrio parahaemolyticus is thought to play an important role in the severe diarrhoea caused by this organism. this study investigated the enterotoxicity of tdh for human intestinal cells. addition of tdh to the mucosal side of human colonic tissue in ussing chambers caused increased short circuit currents (isc), a process that was inhibited by 4,4'-diisothiocyanatostilbene-2,2'-disulphonic acid (dids), an inhibitor of ca2+ -activated chloride (cl- ... | 2000 | 10966228 |
| cl(-) secretion in colonic epithelial cells induced by the vibrio parahaemolyticus hemolytic toxin related to thermostable direct hemolysin. | a hemolytic toxin related to thermostable direct hemolysin (tdh), tdh-related hemolysin (trh), produced by kanagawa-phenomenon-negative vibrio parahaemolyticus is suspected of playing an important, but yet-to-be-elucidated role in diarrhea caused by this organism. in cultured human colonic epithelial cells, trh increases cl(-) secretion, followed by elevation of intracellular calcium. | 2000 | 10948178 |
| the multiple identities of vibrio parahaemolyticus. | vibrio parahaemolyticus is a ubiquitous marine bacterium and human pathogen. the organism possesses multiple cell types appropriate for life under different circumstances. the swimmer cell, with a single polar flagellum, is adapted to life in liquid environments. the polar flagellum is powered by the sodium motive force and can propel the bacterium at fast speeds. the swarmer cell, propelled by many proton-powered lateral flagella, can move through highly viscous environments, colonize surfaces, ... | 1999 | 10941784 |
| isolation of vibrio and pseudomonas from brown shrimp (penaeus californiensis holmes) intestine. | bacteria of the genera vibrio, pseudomonas and aeromonas were isolated from the intestine of apparently healthy brown shrimp (penaeus californiensis holmes) cultured in a tidal pond. species from these genera of bacteria have been reported as shrimp pathogens and have been involved in human gastrointestinal disorders related to seafood consumption. isolation was done first in marine broth, then in selective media (tcbs, cetrimide and macconkey). the oxidase negative strains were discarded as ins ... | 1997 | 10932719 |
| analysis of gyrb and toxr gene sequences of vibrio hollisae and development of gyrb- and toxr-targeted pcr methods for isolation of v. hollisae from the environment and its identification. | isolation of vibrio hollisae strains, particularly from the environment, is rare. this may be due, in part, to the difficulty encountered when using conventional biochemical tests to identify the microorganism. in this study, we evaluated whether two particular genes may be useful for the identification of v. hollisae. the two genes are presumed to be conserved among the bacterial species (gyrb) or among the species of the genus vibrio (toxr). a portion of the gyrb sequence of v. hollisae was cl ... | 2000 | 10919814 |
| characterization of vibrio parahaemolyticus isolates obtained from foodborne illness outbreaks during 1992 through 1995 in taiwan. | vibrio parahaemolyticus is an important foodborne pathogen in taiwan and many other asian countries. a total of 371 isolates of v. parahaemolyticus collected from patients involved in foodborne illness outbreaks in taiwan from 1992 to 1995 were characterized. these isolates had typical biochemical characteristics and only 4% were urease positive. the most frequently isolated serovars were o5:k15 (18.5%), o4:k8 (16.2%), o3:k29 (12.5%), o1:k56 (8.3%), o2:k3 (6.5%), and o4:k12 (6.0%). most of the i ... | 2000 | 10914657 |
| [acute gastroenteritis caused by urease positive vibrio parahaemolyticus in an immunocompromised patient]. | 2000 | 10905019 | |
| vibrio parahaemolyticus, japan, 1996-1998. | 1999 | 10887775 | |
| the use of colony hybridization in the isolation of thermostable direct hemolysin-producing vibrio parahaemolyticus from foods implicated in an incidence of food poisoning. | 2000 | 10871921 | |
| characterization of the vibrio parahaemolyticus na+/glucose cotransporter. a bacterial member of the sodium/glucose transporter (sglt) family. | the vibrio parahaemolyticus sodium/glucose transporter (vsglt) is a bacterial member of the sglt gene family. wild-type and mutant vsglt proteins were expressed in escherichia coli, and transport activity was measured in intact cells and plasma membrane vesicles. two cysteine-less vsglt proteins exhibited sugar transport rates comparable with that of the wild-type protein. six residues in two regions of vsglt known to be of functional importance in sglt1 were replaced individually with cysteine ... | 2000 | 10852908 |
| antibacterial activity of a chitooligosaccharide mixture prepared by cellulase digestion of shrimp chitosan and its application to milk preservation. | the antibacterial activity of a chitooligosaccharide mixture prepared by digestion of shrimp chitosan with cellulase at 50 degrees c for 14 h was evaluated. sugars with 1 to 8 degrees of polymer (dp) were found in this chitooligosaccharide mixture, and the weight percentage of sugars with dp > or = 6 was 44.3%. minimal lethal concentrations of this mixture against aeromonas hydrophila, escherichia coli, listeria monocytogenes, pseudomonas aeruginosa, salmonella typhimurium, shigella dysenteriae, ... | 2000 | 10852568 |
| analysis of the polar flagellar gene system of vibrio parahaemolyticus. | vibrio parahaemolyticus has dual flagellar systems adapted for locomotion under different circumstances. a single, sheathed polar flagellum propels the swimmer cell in liquid environments. numerous unsheathed lateral flagella move the swarmer cell over surfaces. the polar flagellum is produced continuously, whereas the synthesis of lateral flagella is induced under conditions that impede the function of the polar flagellum, e.g., in viscous environments or on surfaces. thus, the organism possess ... | 2000 | 10850984 |
| molecular characterization of vibrio parahaemolyticus vsglt: a model for sodium-coupled sugar cotransporters. | the na(+)/galactose cotransporter (vsglt) of vibrio parahaemolyticus, tagged with c-terminal hexahistidine, has been purified to apparent homogeneity by ni(2+) affinity chromatography and gel filtration. resequencing the vsglt gene identified an important correction: the n terminus constitutes an additional 13 functionally essential residues. the mass of his-tagged vsglt expressed under its native promoter, as determined by electrospray ionization-mass spectrometry (esi-ms), verifies these 13 re ... | 2000 | 10835424 |
| a filamentous phage associated with recent pandemic vibrio parahaemolyticus o3:k6 strains. | a specific serotype, o3:k6, of vibrio parahaemolyticus has recently been causing epidemics of gastroenteritis in southeast asia, japan, and north america. to examine whether the new o3:k6 strains possess characteristics that may exacerbate outbreaks, we compared v. parahaemolyticus o3:k6 strains with non-o3:k6 strains using strains isolated from individuals with traveler's diarrhea at kansai airport quarantine station, osaka, japan. all 24 o3:k6 strains possessed a common plasmid, po3k6 (dna siz ... | 2000 | 10834969 |
| chemical and serological properties of lipopolysaccharides from vibrio parahaemolyticus o-untypeable strains isolated from patients. | chemical and serological studies have been carried out on the o-antigenic lipopolysaccharides (lps) of six strains, u-6443, w-90144, x-3972, ad-7999, 90a-6611 and kx-v212, of vibrio parahaemolyticus isolated from patients. the o-serotypes of these strains have not been identified because they were not agglutinated by any diagnostic antisera against known o-serotype strains. a compositional sugar analysis of their lps revealed that out of the six o-untypeable (out) strains, u-6443, w-90144 and ad ... | 2000 | 10832965 |
| isolation of a pandemic o3:k6 clone of a vibrio parahaemolyticus strain from environmental and clinical sources in thailand. | application of an immunomagnetic enrichment method selective for vibrio parahaemolyticus serovar k6 allowed isolation of a strain belonging to the pandemic o3:k6 clone of v. parahaemolyticus from fresh shellfish not implicated in a clinical case in southern thailand. arbitrarily primed pcr profiles of this strain, clinical o3:k6 strains isolated from sporadic diarrhea cases in the same area, and a standard pandemic o3:k6 strain were indistinguishable. | 2000 | 10831459 |
| vibrio parahaemolyticus infections in the united states, 1973-1998. | vibrio parahaemolyticus infections are associated with consumption of raw or undercooked shellfish, contaminated food, and exposure of wounds to warm seawater. foodborne outbreaks and sporadic infections from vibrio species in 4 gulf coast states are reported routinely to the centers for disease control and prevention (cdc). between 1988 and 1997, 345 sporadic v. parahaemolyticus infections were reported: 59% were gastroenteritis, 34% were wound infections, 5% were septicemia, and 2% were from o ... | 2000 | 10823766 |
| enterotoxicity and cytotoxicity of vibrio parahaemolyticus thermostable direct hemolysin in in vitro systems. | vibrio parahaemolyticus is a marine bacterium known to be a common cause of seafood gastroenteritis worldwide. the thermostable direct hemolysin (tdh) has been proposed to be a major virulence factor of v. parahaemolyticus. tdh causes intestinal fluid secretion as well as cytotoxicity in a variety of cell types. in this study, we investigated the interplay between the hemolysin's enterotoxic and cytotoxic effects by using both human and rat cell monolayers. as revealed by microspectrofluorimetry ... | 2000 | 10816461 |
| some emerging food and water borne pathogens. | emerging pathogens are those infective organisms whose incidence has recently increased or is likely to increase during the next two decades due to changes in demography, food habits, food technology, commerce, water sources and environmental factors. some important emerging food and water borne bacterial pathogens include listeria monocytogenes, campylobacter jejuni, yersinia enterocolitica, salmonella enteritidis, escherichia coli o157: h7, vibrio cholerae biotype e1 tor serotype 0139, vibrio ... | 1999 | 10810592 |
| application of pulsed-field gel electrophoresis to the investigation of a nosocomial outbreak of vibrio parahaemolyticus. | the method of pulsed-field gel electrophoresis (pfge) for vibrio parahaemolyticus was first published in 1996. since then, its application has been seldom reported in the literature. a food poisoning outbreak due to v. parahaemolyticus occurred in five wards of a hospital was investigated using this method. twenty-five patients were involved and all of them had eaten food supplied by the hospital. of the 15 cases whose stools were available for culture, only four cultures yielded v. parahaemolyt ... | 2000 | 10806961 |
| the marine pathogen vibrio vulnificus encodes a putative homologue of the vibrio harveyi regulatory gene, luxr: a genetic and phylogenetic comparison. | vibrio vulnificus is an opportunistic pathogen that exhibits numerous virulence factors, including the secretion of a zinc metalloprotease and the production of a capsule. we have cloned and sequenced a gene from v. vulnificus that is a homologue of the positive transcriptional regulator, luxr, of the lux operon in vibrio harveyi. this gene encodes a putative, single complete open reading frame designated smcr, which shares greater than 75% nucleotide identity with luxr of v. harveyi. the deduce ... | 2000 | 10806366 |
| nhaa, an na(+)/h(+) antiporter involved in environmental survival of vibrio cholerae. | vibrio cholerae, the agent of cholera, is a normal inhabitant of aquatic environments, in which it survives under a wide range of conditions of ph and salinity. in this work, we identified the nhaa gene in a wild-type epidemic strain of v. cholerae o1. nhaa encodes a protein of 382 amino acids that is very similar to the proteins nhaa of vibrio parahaemolyticus, vibrio alginolyticus ( approximately 87% identity), and escherichia coli (56% identity). v. cholerae nhaa complements an e. coli nhaa m ... | 2000 | 10781565 |
| resuscitation of viable but nonculturable cells of vibrio parahaemolyticus induced at low temperature under starvation. | vibrio parahaemolyticus is known to exist in a viable but nonculturable state when incubated at low temperature under starvation. it has long been debated whether the culturable cells which appear after temperature upshift are the result of true resuscitation or regrowth of a few residual culturable cells. starved v. parahaemolyticus cells at 4 degrees c reached the nonculturable stage in about 12 days. the true resuscitation of nonculturable cells of v. parahaemolyticus occurred after spreading ... | 2000 | 10779722 |
| proteomics on full-length membrane proteins using mass spectrometry. | a general technique has been developed that allows rapid mass spectrometric analysis of full-length membrane proteins [whitelegge, j. p., le coutre, j., et al. (1999) proc. natl. acad. sci. u.s.a. 96, 10695-10698]. using in-line hplc electrospray ionization mass spectrometry (lc-ms), different native and recombinant bacterial membrane proteins of up to 61 kda are characterized. mass spectrometric data of four entirely different membrane proteins from three bacterial organisms, two transporters, ... | 2000 | 10757971 |
| effect of mg(2+) ion in protein secretion by magnesium-resistant strains of pseudomonas aeruginosa and vibrio parahaemolyticus isolated from the coastal water of haldia port. | a rapidly growing industrial complex including oil refineries and chemical industries has developed around the coastal area of haldia port in the district of midnapore, west bengal, india. the coastal water is highly polluted with industrial wastes along with petroleum hydrocarbons. the bacteria isolated from the different sites of the coastal waters were escherichia coli, alcaligenes, acinetobacter, klebsiella spp., micrococcus spp., vibrio spp., pseudomonas aeruginosa and vibrio parahaemolytic ... | 2000 | 10754240 |
| distribution of vibrio cholerae virulence genes among different vibrio species isolated in sardinia, italy. | the members of the genus vibrio include harmless aquatic strains as well as strains capable of causing epidemics of cholera. diarrhoea caused by vibrio cholerae is attributed to cholerae enterotoxin (ct) codified by the ctx operon and regulated by a number of virulence genes such as toxt, toxr and toxs. fifty-two vibrio strains were isolated from different aquatic environments in and around sardinia and searched by pcr for the presence of ctxa, zot, ace, toxr, toxs, toxt, tcpa and vpi virulence ... | 2000 | 10747228 |
| emergence and prevalence of a novel vibrio parahaemolyticus o3:k6 clone in japan. | 1999 | 10738364 | |
| incidence of highly genetically diversified vibrio parahaemolyticus in seafood imported from asian countries. | vibrio parahaemolyticus is an important pathogen of humans and aquacultured animals, especially in asian countries. in this study, we examined 686 samples of seafood imported from hong kong, indonesia, thailand and vietnam for v. parahaemolyticus. v. parahaemolyticus was recovered from 315 (45.9%) samples. the incidence of v. parahaemolyticus in products from hong kong and thailand was markedly higher than the incidence in products from indonesia and vietnam. the incidence rates in shrimp, crab, ... | 1999 | 10733249 |
| cloning and characterization of manganese superoxide dismutase gene from vibrio parahaemolyticus and application to preliminary identification of vibrio strains. | the soda gene coding for manganese superoxide dismutase (mn-sod) from the marine microorganism vibrio parahaemolyticus was cloned, sequenced, and overexpressed in escherichia coli by use of the pet20b (+) expression vector. the full-length gene consisted of a 588-bp open reading frame and encoded a polypeptide of 196 amino acid residues, with a calculated molecular mass of 21,713 da. the recombinant enzyme was efficiently purified from crude e. coli cell lysate by metal ion affinity chromatograp ... | 1999 | 10690650 |
| pandemic spread of an o3:k6 clone of vibrio parahaemolyticus and emergence of related strains evidenced by arbitrarily primed pcr and toxrs sequence analyses. | vibrio parahaemolyticus o3:k6 strains responsible for the increase in the number of cases of diarrhea in calcutta, india, beginning in february 1996 and those isolated from southeast asian travelers beginning in 1995 were shown to belong to a unique clone characterized by possession of the tdh gene but not the trh gene and by unique arbitrarily primed pcr (ap-pcr) profiles (j. okuda, m. ishibashi, e. hayakawa, t. nishino, y. takeda, a. k. mukhopadhyay, s. garg, s. k. bhattacharya, g. b. nair, an ... | 2000 | 10655349 |
| bactericidal activities of essential oils of basil and sage against a range of bacteria and the effect of these essential oils on vibrio parahaemolyticus. | basil and sage essential oils were examined for bactericidal activity against a range of gram-positive and gram-negative bacteria by viable count determinations. generally, gram-positive bacteria showed higher resistance to basil and sage essential oils than gram-negative bacteria. vibrio species showed a high sensitivity to both essential oils. stationary growth phase cells of selected bacteria showed higher resistance to these essential oils than exponential growth phase cells. basil-resistant ... | 1999 | 10652788 |
| insertional inactivation of genes encoding components of the sodium-type flagellar motor and switch of vibrio parahaemolyticus. | vibrio parahaemolyticus possesses two types of flagella, polar and lateral, powered by distinct energy sources, which are derived from the sodium and proton motive forces, respectively. although proton-powered flagella in escherichia coli and salmonella enterica serovar typhimurium have been extensively studied, the mechanism of torque generation is still not understood. molecular knowledge of the structure of the sodium-driven motor is only now being developed. in this work, we identify the swi ... | 2000 | 10648530 |
| fulminant necrotising fasciitis caused by vibrio parahaemolyticus. | we report a patient with septicaemia and fulminant necrotising fasciitis caused by vibrio parahaemolyticus. this organism is strongly associated with seawater exposure and seafood ingestion. the patient recovered due to expedient management, prompt recognition of the organism, appropriate antimicrobial cover and surgical debridement. the lesson to be learned is that this organism should be clinically suspected and recognised from its typical history of injury and fulminant clinical progress as a ... | 1999 | 10628251 |
| [characterization of haemolysis of the vibrio parahaemolyticus no.93]. | vibrio parahaemolyticus is a causative bacterium of food poisoning, and the haemolysin produced by this organism has been considered as one of the important virulence factors. in order to understand the pathogenic mechanism of this bacterium, the characteristics of haemolysin from vibrio parahaemolyticus isolated from taiwan were studied. one of the clinical strains, v. parahaemolyticus no.93, presents a weak hemolytic zone on 7% nacl-wagatsuma medium. the dna hybridization results show that v. ... | 1997 | 10592808 |
| [k-serotype analyses of vibrio parahaemolyticus isolated in northern taiwan, 1983 through 1993]. | from 1983 through 1993, 786 strains of vibrio parahaemolyticus were collected from food-borne disease outbreaks and sporadic cases of diarrheal illness in northern taiwan, involving 42 k-serotypes. five top leading serotypes were k8 (36.8%), k15 (10.8%), k12 (8.7%), k56 (7.9%) and k63 (4.7%). however, a variation of k-serotypes was found during this study period. from 112 food-borne outbreaks associated with this microorganism, only 54 (48.2%) outbreaks were caused by a single serotype, while 58 ... | 1996 | 10592803 |
| survey on the distribution of vibrionaceae at the seaport areas in taiwan, 1991-1994. | a monthly survey on the distribution of human-pathogenic vibrionaceae of the seawater from five principal harbors in taiwan was conducted by national quarantine service from july, 1991 to february, 1994. of the total 1,167 vibrionaceae isolates, strains of vibrio alginolyticus (449 strains) were the most frequently isolated, followed by vibrio parahaemolyticus (262) , aeromonas hydrophila (153), vibrio cholerae non-o1 (86), and vibrio vulnificus (67). none of vibrio cholerae o1 was isolated. the ... | 1996 | 10592802 |
| vibrio parahemolyticus bacteremia: case report. | vibrio parahemolyticus (v. parahemolyticus) is a halophilic gram-negative bacillus that lives in the ocean. it is the leading cause of infectious diarrhea in taiwan and sometimes produces soft tissue infections, but it is rarely a cause of bacteremia. there have been only 11 cases reported in the literature. most of the cases involved a history of ingestion of seafood or exposure to seawater. in addition, those patients were all immunosuppressed, especially with leukemia and cirrhosis. we report ... | 1999 | 10584427 |
| [analysis of thermostable direct hemolysin-producing vibrio parahaemolyticus by pulsed-field gel electrophoresis]. | we investigated the source of thermostable direct hemolysin-producing vibrio parahaemolticus infection (positive strains) that causes vibrio parahaemolticus food poisoning. | 1999 | 10565185 |
| siderophore production of vibrio parahaemolyticus strains from different sources. | vibrio parahaemolyticus strains isolated from different sources were assayed for their ability to produce a siderophore, vibrioferrin, under iron-limited growth conditions. the mean value +/- standard error of mean (microm vibrioferrin in spent culture supernatant/optical density at 660 nm) was 832.3 +/- 66.9 for clinical isolates (n=44), which was significantly higher (p<0.01) than those for food isolates (461.0 +/- 66.5; n=37) and coastal isolates (378.8 +/- 37.2; n=26). this suggests that gre ... | 1999 | 10553685 |
| vibriophage kvp40 and coliphage t4 genomes share a homologous 7-kbp region immediately upstream of the gene encoding the major capsid protein. | vibriophage kvp40, a large tailed dna phage morphologically similar to t-even coliphages, has a major capsid protein (mcp) homologous to the equivalent protein, gp23(*), of coliphage t4. the sequence analysis was extended to a 7-kbp region immediately upstream of the mcp gene encoding the precursor of mcp. the region as a whole was fairly homologous to the corresponding region of the t4 genome and contained 8 orfs homologous to t4 genes 17, 18, 19, 20, 67, 68, 21, and 22 in the same order as in ... | 1999 | 10550672 |
| update on emerging infections from the centers for disease control and prevention. outbreak of vibrio parahaemolyticus infection associated with eating raw oysters and clams harvested from long island sound--connecticut, new jersey, and new york, 1998. | this article is part of a regular series on emerging infections from the centers for disease control and prevention (cdc) and the emergency id net, an emergency department-based and cdc-collaborative surveillance network. important infectious disease public health information with relevance to emergency physicians is reported. the goal of this series is to advance knowledge about communicable diseases in emergency medicine, and foster cooperation between the front line of clinical medicine and p ... | 1999 | 10533020 |
| ribotyping of vibrio parahaemolyticus isolates obtained from food poisoning outbreaks in taiwan. | vibrio parahaemolyticus is a prevalent food-borne pathogen in taiwan, japan and other asian countries. this work presents a novel ribotyping method for the molecular epidemiological examination of this pathogen. genomic dna was fragmented by hindiii digestion and hybridized with cdna probe for escherichia coli 16s and 23s rna genes. a total of 121 isolates obtained from outbreaks during 1992 and 1994 in taiwan were characterized by this ribotyping method. four to seventeen restricted fragments w ... | 1999 | 10529103 |
| on the in-vitro antimicrobial activity of oleuropein and hydroxytyrosol. | secoiridoides (oleuropein and derivatives), one of the major classes of polyphenol contained in olives and olive oil, have recently been shown to inhibit or delay the rate of growth of a range of bacteria and microfungi but there are no data in the literature concerning the possible employment of these secoiridoides as antimicrobial agents against pathogenic bacteria in man. in this study five atcc standard bacterial strains (haemophilus influenzae atcc 9006, moraxella catarrhalis atcc 8176, sal ... | 1999 | 10504039 |
| comparison of effects of wasabia japonica and allyl isothiocyanate on the growth of four strains of vibrio parahaemolyticus in lean and fatty tuna meat suspensions. | lean tuna meat suspensions (lean), with a fat content of 0.006%, and fatty tuna meat suspension (fatty), with a fat content of 3.0% were inoculated with four strains of vibrio parahaemolyticus and wasabi (wasabia japonica matsumura) or allyl isothiocyanate (ait) was added before incubation at 37 degrees c. during the incubation, viable vibrio counts were determined on tcbs agar plates. both lean and fatty suspensions were inoculated with v. parahaemolyticus aoto-81, (1.28+/-0.20) x 10(2) cfu/ml, ... | 1999 | 10477067 |
| geographical features of estuaries for neritid gastropods including clithon retropictus to preserve thermostable direct hemolysin-producing vibrio parahaemolyticus. | thermostable direct hemolysin-producing strain of vibrio parahaemolyticus was not detected from the alimentary tract of 7 neritid gastropods including clithon retropictus at 9 estuaries of southwest islands in japan in the present study. the strain has been detected from c. retropictus at 2 estuaries facing the sea of japan but not at 2 estuaries facing the seto inland sea and the pacific ocean in western japan in our previous studies. in comparison with geographical features of the estuaries wh ... | 1999 | 10423701 |
| antibacterial activity of marine-derived fungi. | a total of 227 marine isolates of ubiquitous fungi were cultivated on different media and the secondary metabolite content of the extracts (ethyl acetate/chloroform/methanol 3:2:1) characterized by hplc. the fungi were secured from animals, plants and sediments of venezuelan waters (0-10 m) including mangroves and lagoonal areas. the extracts were tested for antibacterial activity. a total of 7 were active towards vibrio parahaemolyticus and 55 towards staphylococcus aureus, representing 18 diff ... | 1998 | 10409050 |
| comparison of molecular methods for typing vibrio parahaemolyticus. | an outbreak of vibrio parahaemolyticus gastroenteritis on canada's west coast in 1997 emphasized the need to develop molecular methods for differentiation and typing of these organisms. isolates were analyzed by enterobacterial repetitive intergenic consensus sequence (eric) pcr, detection of restriction fragment length polymorphisms (rflp) in rrna genes (ribotyping), pulsed-field gel electrophoresis (pfge), and rflp analysis of the genetic locus encoding the polar flagellum (fla locus rflp anal ... | 1999 | 10405387 |
| factors influencing in vitro killing of bacteria by hemocytes of the eastern oyster (crassostrea virginica). | a tetrazolium dye reduction assay was used to study factors governing the killing of bacteria by oyster hemocytes. in vitro tests were performed on bacterial strains by using hemocytes from oysters collected from the same location in winter and summer. vibrio parahaemolyticus strains, altered in motility or colonial morphology (opaque and translucent), and listeria monocytogenes mutants lacking catalase, superoxide dismutase, hemolysin, and phospholipase activities were examined in winter and su ... | 1999 | 10388697 |
| a filamentous phage of vibrio parahaemolyticus o3:k6 isolated in laos. | a filamentous phage, 'lvpf5,' of vibrio parahaemolyticus o3:k6 strain lvp5 was isolated and characterized. the host range was not restricted to serotype o3:k6, but 7 of 99 v. parahaemolyticus strains with a variety of serotypes were susceptible to the phage. the phage was inactivated by heating at 80 c for 10 min and by treating with chloroform. sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the phage exhibited a 3.8 kda protein. the amino-terminal amino acid sequence of the coat p ... | 1999 | 10385206 |
| detection of total and hemolysin-producing vibrio parahaemolyticus in shellfish using multiplex pcr amplification of tl, tdh and trh. | vibrio parahaemolyticus is an important human pathogen which can cause gastroenteritis when consumed in raw or partially-cooked seafood. a multiplex pcr amplification-based detection of total and virulent strains of v. parahaemolyticus was developed by targeting thermolabile hemolysin encoded by tl, thermostable direct hemolysin encoded by tdh, and thermostable direct hemolysin-related trh genes. following optimization using oligonucleotide primers targeting tl, tdh and trh genes, the multiplex ... | 1999 | 10379807 |
| clonal diversity among recently emerged strains of vibrio parahaemolyticus o3:k6 associated with pandemic spread. | the genomes of the o3:k6 strains of vibrio parahaemolyticus which abruptly emerged in calcutta, india, in february 1996 and which demonstrated an unusual potential to spread and an enhanced propensity to cause infections were examined by different molecular techniques to determine clonality. no restriction fragment length polymorphism (rflp) in the gene encoding the thermostable direct hemolysin was observed among the o3:k6 isolates of v. parahaemolyticus. clonal diversity among the o3:k6 strain ... | 1999 | 10364615 |
| [food poisonings caused by halophilic vibrios in the city of vladivostok: their epidemiological characterization]. | 1999 | 10356733 | |
| sequence of the v. parahaemolyticus gene for cytoplasmic n, n'-diacetylchitobiase and homology with related enzymes. | the nucleotide sequence of the gene encoding the cytoplasmic n, n'-diacetylchitobiase [ec 3.2.1.14] from vibrio parahaemolyticus (atcc #27969) has been determined. the deduced peptide sequence of this unusual beta-hexosaminidase surprisingly shows minimum evolutionary relationship to two other reported n, n'-diacetylchitobiases from vibrios, except in highly conserved regions which are also homologous to lysosomal beta-hexosaminidases from eukaryotes including humans. in contrast, the two other ... | 1999 | 10348911 |
| comparison of the heme iron utilization systems of pathogenic vibrios. | vibrio alginolyticus, vibrio fluvialis, and vibrio parahaemolyticus utilized heme and hemoglobin as iron sources and contained chromosomal dna similar to several vibrio cholerae heme iron utilization genes. a v. parahaemolyticus gene that performed the function of v. cholerae huta was isolated. a portion of the tonb1 locus of v. parahaemolyticus was sequenced and found to encode proteins similar in amino acid sequence to v. cholerae hutw, tonb1, and exbb1. a recombinant plasmid containing the v. ... | 1999 | 10348876 |
| response of pathogenic vibrio species to high hydrostatic pressure. | vibrio parahaemolyticus atcc 17802, vibrio vulnificus atcc 27562, vibrio cholerae o:1 atcc 14035, vibrio cholerae non-o:1 atcc 14547, vibrio hollisae atcc 33564, and vibrio mimicus atcc 33653 were treated with 200 to 300 mpa for 5 to 15 min at 25 degrees c. high hydrostatic pressure inactivated all strains of pathogenic vibrio without triggering a viable but nonculturable (vbnc) state; however, cells already existing in a vbnc state appeared to possess greater pressure resistance. | 1999 | 10347079 |
| occurrence, diversity, and pathogenicity of halophilic vibrio spp. and non-o1 vibrio cholerae from estuarine waters along the italian adriatic coast. | the occurrence, diversity, and pathogenicity of vibrio spp. were investigated in two estuaries along the italian adriatic coast. vibrio alginolyticus was the predominant species, followed by vibrio parahaemolyticus, non-o1 vibrio cholerae, and vibrio vulnificus. by using a biochemical fingerprinting method, all isolates were grouped into nine phenotypes with similarity levels of 75 to 97.5%. the production of toxins capable of causing cytoskeleton-dependent changes was detected in a large number ... | 1999 | 10347072 |
| characterization of the antiseptic-resistance gene qace delta 1 isolated from clinical and environmental isolates of vibrio parahaemolyticus and vibrio cholerae non-o1. | the nucleotide sequence and mechanism of action were examined on the antiseptic-resistance gene qace delta 1 that had been isolated from pseudomonas aeruginosa, vibrio parahaemolyticus and vibrio cholerae non-o1. the nucleotide sequences of qace delta 1 genes isolated from environmental isolates of v. cholerae non-o1 and v. parahaemolyticus differed by one base from that of the gene from p. aeruginosa. escherichia coli c600 that harbored qace delta 1 genes from several strains of vibrio spp. exh ... | 1999 | 10339831 |
| disappearance of glyceraldehyde 3-phosphate dehydrogenase from erythrocyte membrane by hemolysis with thermostable direct hemolysin of vibrio parahaemolyticus or vibrio cholerae el tor hemolysin. | it is believed that the thermostable direct hemolysin (tdh) of vibrio parahaemolyticus and el tor hemolysin (eth) of vibrio cholerae damage erythrocytes and other cells by acting as pore-forming toxins. in this study, we found that a protein band with a molecular weight of 37,000 daltons specifically disappeared from erythrocyte membrane after hemolysis by tdh and eth, but not after hypotonic hemolysis. the 37 kda band was identified as glyceraldehyde 3-phosphate dehydrogenase (g3pd), a glycolyt ... | 1999 | 10338202 |
| molecular typing of vibrio parahaemolyticus isolates, obtained from patients involved in food poisoning outbreaks in taiwan, by random amplified polymorphic dna analysis. | vibrio parahaemolyticus is one of the most important food-borne pathogens in taiwan, japan, and other countries with long coastlines. this paper reports on the development of a new random amplified polymorphic dna (rapd) method for the molecular typing of this pathogen. the 10-mer primer 284 (5'-cag gcg cac a-3') was selected to generate polymorphic amplification profiles of the genomic dna at an annealing temperature of 38 degrees c. a total of 308 clinical isolates of v. parahaemolyticus colle ... | 1999 | 10325328 |
| [a study on the distribution and etiology of pathogenic vibrio in the 5 coastal provinces of china]. | in order to understand the distribution and pathogenicity of vibrio in the coastal areas of china a study consisted of 51 water samples and foodstuff from 10 different areas in 5 provinces was conducted microbiologically and etiologically. results showed that 45 pathogenic vibrio were identified, with majority of a. caviae (26.7%), v. alginolyticus (22.2%) and v. parahaemolyticus (11.1%), same kinds of vibrio found in feces from patients with acute infectious diarrhea. prevalence rates of pathog ... | 1998 | 10322692 |
| mutations conferring resistance to phenamil and amiloride, inhibitors of sodium-driven motility of vibrio parahaemolyticus. | the bacterial flagellum is powered by a rotary motor capable of turning the helical flagellar propeller at very high speeds. energy to drive rotation is derived from the transmembrane electrochemical potential of specific ions. ions passing through a channel component are thought to generate the force to power rotation. two kinds of motors, dependent on different coupling ions, have been described: proton-driven and sodium-driven motors. there are four known genes encoding components of the sodi ... | 1999 | 10318954 |
| sequence analysis of the gyra and parc homologues of a wild-type strain of vibrio parahaemolyticus and its fluoroquinolone-resistant mutants. | vibrio parahaemolyticus causes seafood-borne gastroenteritis in humans. it is particularly important in japan, where raw seafood is frequently consumed. fluoroquinolone is one of the current drugs of choice for treating patients infected by v. parahaemolyticus because resistant strains are rarely found. to study a possible fluoroquinolone resistance mechanism in this organism, nucleotide sequences that are homologous to known gyra and parc genes have been cloned from v. parahaemolyticus aq3815 a ... | 1999 | 10223929 |
| macromolecular antimicrobial glycoprotein, achacin, expressed in a methylotrophic yeast pichia pastoris. | a cdna encoding achacin, an antimicrobial glycoprotein from the body surface mucus of giant african snail achacina fulica férussac, was expressed in a methylotrophic yeast, pichia pastoris, and recombinant achacin (rach) was secreted in yeast minimal medium in a polyglycosylated form with 80 kda. carbohydrate analysis revealed that the glycosylated moiety of rach was composed of 50 mol mannose and 2 mol n-acetylglucosamine residues. antimicrobial activity using escherichia coli and staphylococcu ... | 1999 | 10217406 |
| [comparison of epidemiological markers for vibrio parahaemolyticus isolated from food poisoning]. | vibrio parahaemolyticus food poisoning, is the most prevalent among bacterial food poisoning in japan. study of epidemiologic markers is important in an attempt to trace the source of contamination. the purpose of this study was to compare seven different typing methods (serotyping, plasmid profile, antibiogram, phage susceptibility. tdh production, tdh and trh gene and pulsed-field gel electrophoresis [pfge]) for v. parahaemolyticus. outbreaks of v. parahaemolyticus food poisoning which occurre ... | 1999 | 10213996 |
| [bacteriological studies of traveller's diarrhoea (6). analysis of enteropathogenic bacteria at kansai airport quarantine station from september 4th, 1994 through december 1996]. | during the period of investigation from sept. 4, 1994 to dec, 1996, a total of 11,446,534 overseas travellers were quarantined at kansai airport quarantine station, and 22,187 voluntarily reported of episodes suffering from diarrhoea. bacteriological examination of the stools a total of 9,299 individuals' was performed, and the following results were obtained. 1) various enteropathogenic bacteria were isolated from 33.3% of the stools examined. bacterial species isolated were as follows: plesiom ... | 1999 | 10213987 |
| expression and characterization of the prtv gene encoding a collagenase from vibrio parahaemolyticus in escherichia coli. | the prtv gene, encoding a collagenase of vibrio parahaemolyticus, was expressed in escherichia coli and purified by affinity chromatography. the transformant e. coli bl21(de3)(pprt2) secreted the recombinant prtv, and the highest enzyme activity was detected in the culture supernatant after 5 h iptg induction. the molecular mass of purified prtv was 62 kda as determined by gel filtration, which was similar to that obtained by sds-page (64 kda). this suggested that prtv was a monomer protein havi ... | 1999 | 10206692 |
| physical and genetic map of the genome of vibrio parahaemolyticus: presence of two chromosomes in vibrio species. | we constructed a physical map of the genomic dna (5.1 mb) for vibrio parahaemolyticus strain aq4673 by combining 17 adjacent noti fragments. this map shows two circular replicons of 3.2 and 1.9 mb. pulsed-field gel electrophoresis (pfge) of undigested genomic dna revealed two bands of corresponding sizes. analysis both by noti digestion and by southern blot of the two isolated bands confirmed the existence of two replicons. the presence of genes for 16s rrna on both the replicons indicates that ... | 1999 | 10200969 |
| [vibrio parahaemolyticus enteritis]. | 1999 | 10088453 | |
| identification of vibrio parahaemolyticus strains at the species level by pcr targeted to the toxr gene. | the dna colony hybridization test with the polynucleotide probe for vibrio parahaemolyticus toxr gene was performed. all 373 strains of v. parahaemolyticus gave positive results, and the strains belonging to four other vibrio species including vibrio alginolyticus gave weakly positive results, suggesting that toxr sequence variation may reflect the phylogenetic relationships of vibrio species. we then established a toxr-targeted pcr protocol for the specific detection of v. parahaemolyticus. | 1999 | 10074546 |
| isolation and characterization of vibrio parahaemolyticus causing infection in iberian toothcarp aphanius iberus. | high mortality among laboratory cultured iberian toothcarp aphanius iberus occurred in february 1997 in valencia (spain). the main signs of the disease were external haemorrhage and tail rot. bacteria isolated from internal organs of infected fish were biochemically homogeneous and identified as vibrio parahaemolyticus. the bacteria were haemolytic against erythrocytes from eel anguilla anguilla, amberjack seriola dumerili, toothcarp a. iberus and humans, and were kanagawa-phenomenon-negative. i ... | 1999 | 10073316 |
| randomly amplified polymorphic dna analysis of clinical and environmental isolates of vibrio vulnificus and other vibrio species. | vibrio vulnificus is an estuarine bacterium that is capable of causing a rapidly fatal infection in humans. a randomly amplified polymorphic dna (rapd) pcr protocol was developed for use in detecting v. vulnificus, as well as other members of the genus vibrio. the resulting rapd profiles were analyzed by using rflpscan software. this rapd method clearly differentiated between members of the genus vibrio and between isolates of v. vulnificus. each v. vulnificus strain produced a unique band patte ... | 1999 | 10049874 |
| evaluation of alkaline phosphatase- and digoxigenin-labelled probes for detection of the thermolabile hemolysin (tlh) gene of vibrio parahaemolyticus. | the biochemical identification and enumeration of vibrio parahaemolyticus as described in the fda bacteriological analytical manual is expensive and labour-intensive. to reduce the time and effort necessary to verify the identity of v. parahaemolyticus, the use of a thermolabile haemolysin (tlh) gene probe is proposed. an alkaline phosphatase (ap)-labelled probe was evaluated for specificity against 26 strains of v. parahaemolyticus, 88 strains of other vibrio species and 10 strains of non-vibri ... | 1999 | 10030035 |
| from the centers for disease control and prevention. outbreak of vibrio parahaemolyticus infection associated with eating raw oysters and clams harvested from long island sound--connecticut, new jersey, and new york, 1998. | 1999 | 10029109 | |
| vibrio parahaemolyticus thermostable direct hemolysin modulates cytoskeletal organization and calcium homeostasis in intestinal cultured cells. | vibrio parahaemolyticus is a marine bacterium known to be the leading cause of seafood gastroenteritis worldwide. a 46-kda homodimer protein secreted by this microorganism, the thermostable direct hemolysin (tdh), is considered a major virulence factor involved in bacterial pathogenesis since a high percentage of strains of clinical origin are positive for tdh production. tdh is a pore-forming toxin, and its most extensively studied effect is the ability to cause hemolysis of erythrocytes from d ... | 1999 | 10024554 |
| outbreak of vibrio parahaemolyticus infection associated with eating raw oysters and clams harvested from long island sound--connecticut, new jersey, and new york, 1998. | during july-september 1998, an outbreak of vibrio parahaemolyticus infections associated with consumption of oysters and clams harvested from long island sound occurred among residents of connecticut, new jersey, and new york. this is the first reported outbreak of v. parahaemolyticus linked to consumption of shellfish harvested from new york waters. this report summarizes the investigation of this outbreak. | 1999 | 9935142 |
| a mechanism of resistance to hydrogen peroxide in vibrio rumoiensis s-1. | a possible mechanism of resistance to hydrogen peroxide (h2o2) in vibrio rumoiensis, isolated from the h2o2-rich drain pool of a fish processing plant, was examined. when v. rumoiensis cells were inoculated into medium containing either 5 mm or no h2o2, they grew in similar manners. a spontaneous mutant strain, s-4, derived from v. rumoiensis and lacking catalase activity did not grow at all in the presence of 5 mm h2o2. these results suggest that catalase is inevitably involved in the resistanc ... | 1999 | 9872762 |
| crayfish: a newly recognized vehicle for vibrio infections. | we conducted a 1-year case-control study of sporadic vibrio infections to identify risk factors related to consumption of seafood products in two coastal areas of louisiana and texas. twenty-six persons with sporadic vibrio infections and 77 matched controls were enrolled. multivariate analysis revealed that crayfish (p < 0.025) and raw oysters (p < 0.009) were independently associated with illness. species-specific analysis revealed an association between consumption of cooked crayfish and vibr ... | 1998 | 9825776 |
| [factors of persistence and (or) pathogenicity in vibrios and aeromonads belonging to different ecotopes]. | factors of persistence and/or pathogenicity in vibrio parahaemolyticus and aeromonas hydrophila (hemolytic, lipase, lecithin, dnaase, rnaase, antilysozyme, "anti-interferon", anticomplementary activities and capacity for absorbing congo red) were studied. the study revealed the interspecific and subpopulation (hospital and extraorganismal parts of the population) differences in the activity of the manifestation of these factors. strong dependence of the whole complex of persistence and pathogeni ... | 1998 | 9825495 |
| manifestation of the kanagawa phenomenon, the virulence-associated phenotype, of vibrio parahaemolyticus depends on a particular single base change in the promoter of the thermostable direct haemolysin gene. | thermostable direct haemolysin of vibrio parahaemolyticus has been shown to be a major virulence factor. the kanagawa phenomenon (kp), haemolysis induced by this haemolysin on a special blood agar medium, is strongly associated with clinical strains. we have been studying the expressions of various tdh genes encoding this haemolysin to elucidate the significance of the tdh genes possessed by kp-negative strains isolated from patients. we examined the importance of the promoter sequence variation ... | 1998 | 9822816 |
| bacterial enteric pathogens in uncooked foods in thai markets. | in developing countries, the morbidity and mortality rates of gastrointestinal tract infections from food borne bacteria have been difficult to establish. most studies have only been able to gather data prospectively from isolated geographic sources, rather than from large point-source epidemics. this study investigates the types of bacterial enteric pathogens found in food that was collected in a community in western thailand, where sporadic cases of hemolytic uremia syndrome and cholera have b ... | 1994 | 9815313 |
| cloning and sequencing of major capsid protein (mcp) gene of a vibriophage, kvp20, possibly related to t-even coliphages. | a large, tailed, prolate-headed vibriophage designated kvp20 was isolated from seawater. kvp20 was morphologically very similar to the previously described vibriophage, kvp40 (matsuzaki, s., inoue, t., tanaka, s., 1998. virology, 242, 314-318). however, they showed entirely different host specificities and could easily be differentiated from each other by their patterns of dna restriction fragments. the major capsid protein (mcp) gene of kvp20 encoding the precursor of major capsid protein (pro- ... | 1998 | 9813227 |
| role of outer membrane proteins on the adherence of vibrio parahaemolyticus to rabbit intestinal epithelial cell in vitro. | antiserum against outer membrane preparation of a kanagawa phenomenon-positive strain of vibrio parahaemolyticus were raised in rabbits and absorbed with their lipopolysaccharide. the anti-outer membrane protein serum and its fab (igg) fragment inhibited the adherence of kanagawa-positive strains to rabbit intestinal epithelial cells in vitro. preincubation of rabbit intestinal epithelial cells with outer membrane preparation also inhibited the adherence of these bacteria. anti-lipopolysaccharid ... | 1995 | 9810668 |
| detection of tdh and trh genes in a urea-hydrolysing environmental isolate of vibrio parahaemolyticus from the andamans. | co-existence of trh gene and urea-hydrolysing property in one of 44 marine water isolates of vibrio parahaemolyticus correlates strongly with both genotypic and phenotypic characteristics of the bacterium. thus, urease-producing phenotype can be considered a marker of virulence for the production of thermostable direct haemolysin-related haemolysin (trh) (i.e. possession of trh gene). the same isolate also possessed the tdh gene. an environmental isolate possessing all the characteristics of a p ... | 1998 | 9805414 |
| survival of vibrio cholerae 01 strains in shrimp subjected to freezing and boiling. | this research was undertaken to assess the resistance of vibrio cholerae 01 strains inoculated into white shrimp, penaeus schimitti, to heating and freezing treatments. shrimp samples with and without carapace were obtained from sao luis, brazil. microbial analysis revealed the presence of marine vibrios including vibrio alginolyticus, vibrio parahaemolyticus, and other vibrios and aerobic gram-negative and gram-positive bacteria that grew on selective medium, thiosulfate-citrate-bile salt-sucro ... | 1998 | 9798148 |
| the world's mine oyster. | 1998 | 9787608 | |
| close proximity of the tdh, trh and ure genes on the chromosome of vibrio parahaemolyticus. | the distribution and location of the virulence-factor genes of vibrio parahaemolyticus, tdh and trh, and the structural gene of urease, urec, were examined on the genomic dnas of 115 clinical isolates of v. parahaemolyticus. the majority of strains (81%) had two copies of tdh on the chromosome, and no copies of trh or ure. southern hybridization with a tdh probe, after pulsed-field gel electrophoresis of notl-digested genomic dna of each strain revealed only single bands, suggesting that the two ... | 1998 | 9782499 |
| characterization of the manganese-resistant mutants derived from vibrio parahaemolyticus. | the virulence and some related factors of vibrio parahaemolyticus are regulated by the level of iron. in this study, five mn-resistant mutants were selected after n-methyl-n'-nitrosoguanidine treatment and two transfers in medium containing high levels of manganese chloride. production of siderophores and the 77-kda iron-regulated outer-membrane protein and the bacterial growth in these mn-resistants were deregulated, as compared with the wild-type strain. in addition, the regulation of these ph ... | 1995 | 9775003 |
| the drosophila pax gene eye gone is required for embryonic salivary duct development. | what are the developmental mechanisms required for conversion of an undifferentiated, two-dimensional field of cells into a patterned, tubular organ? in this report, we describe the contribution of the drosophila pax gene eye gone to the development of the embryonic salivary glands and ducts. eye gone expression in salivary tissues is controlled by several known regulators of salivary fate. after the initial establishment of the salivary primordium by sex combs reduced, fork head excludes eye go ... | 1998 | 9753671 |
| filamentous bacteriophages of vibrio parahaemolyticus as a possible clue to genetic transmission. | we have previously reported the isolation and characterization of two filamentous bacteriophages of vibrio parahaemolyticus, designated vf12 and vf33. in this study, to understand the potential of these phages as tools for genetic transmission, we investigated the gene structures of replicative-form (rf) dnas of their genomes and the distribution of these dnas on chromosomal and extrachromosomal dnas. the 7,965-bp nucleotide sequences of vf12 and vf33 were determined. an analysis of the overall ... | 1998 | 9748441 |
| investigation of the 1994-5 ukrainian vibrio cholerae epidemic using molecular methods. | thirty-seven vibrio cholerae and four non-cholera vibrio isolates from ukraine, including strains from the epidemic of 1994-5, were analysed by molecular methods. results from pfge and ribotyping indicated that all ukrainian toxigenic v. cholerae were closely related to each other and to an isolate from a patient from pakistan. a non-toxigenic river water strain obtained during the height of the epidemic was more distantly related to these v. cholerae strains, while the vibrio parahaemolyticus i ... | 1998 | 9747752 |
| identification and characterization of a protease produced by vibrio parahaemolyticus in iron-limited medium. | two proteolytic proteins (about 43 and 90 kda) were produced by clinical strains of vibrio parahaemolyticus cultured in iron-limited medium. the 43 kda-protease was partially purified by ammonium sulfate precipitation, ultrafiltration fractionation and deae-sephacel chromatography. this protease had an optimum ph range of 7 to 8, and an optimum reaction temperature of about 40 degrees c. it was heat-labile, being partially inactivated by heat-treatment at 60 or 90 degrees c for 10 min. the prote ... | 1994 | 9747347 |
| note: molecular cloning of chitinase genes from vibrio anguillarum and v. parahaemolyticus. | chitinase genes from vibrio anguillarum kv9001 and v. parahaemolyticus atcc17802 were cloned into escherichia coli. open reading frames of chitinase genes from v. anguillarum (vac) and v. parahaemolyticus (vpc) are 1755 bp and 1890 bp, respectively. the deduced amino acid sequences of these genes have 71.6% identity. there are two consensus sequence regions in the vac and vpc proteins. the vac gene was highly prevalent in v. anguillarum, and the dna probe of the vac gene hybridized to v. alginol ... | 1998 | 9717305 |
| [bacteriological and virological studies on the cause of sporadic acute gastroenteritis in tama, tokyo (1991-1996)]. | between may 1991 and december 1996, bacteriological and virological investigations on the fecal samples from patient of infantile diarrhea (155 cases), or infectious gastroenteritis (1,409 cases) diagnosed with 9 medical institutions in tama of tokyo were carried out. of 1,564 samples, 722 (46.2%) were enteropathogen positive cases, and mixed infection was also observed in about 15% of the positive cases. among 13 different kinds of enteropathogens identified, the most prevalent one was pathogen ... | 1998 | 9695470 |
| from the centers for disease control and prevention. outbreak of vibrio parahaemolyticus infections associated with eating raw oysters--pacific northwest, 1997. | 1998 | 9669774 | |
| norm, a putative multidrug efflux protein, of vibrio parahaemolyticus and its homolog in escherichia coli. | we found that cells of vibrio parahaemolyticus possess an energy-dependent efflux system for norfloxacin. we cloned a gene for a putative norfloxacin efflux protein from the chromosomal dna of v. parahaemolyticus by using an escherichia coli mutant lacking the major multidrug efflux system acrab as the host and sequenced the gene (norm). cells of e. coli transformed with a plasmid carrying the norm gene showed elevated energy-dependent efflux of norfloxacin. the transformants showed elevated res ... | 1998 | 9661020 |
| [a study on chemical and immunochemical properties and chemotaxonomy of lipopolysaccharides of vibrionaceae, in particular vibrio cholerae and v. parahaemolyticus]. | 1998 | 9654940 | |
| [wound infection caused by vibrio parahaemolyticus]. | 1998 | 9646572 | |
| outbreak of vibrio parahaemolyticus infections associated with eating raw oysters--pacific northwest, 1997. | during july-august 1997, the largest reported outbreak in north america of culture-confirmed vibrio parahaemolyticus infections occurred. illness in 209 persons was associated with eating raw oysters harvested from california, oregon, and washington in the united states and from british columbia (bc) in canada; one person died. this report summarizes the investigations of the outbreak, which suggest that elevated water temperatures may have contributed to increased cases of illness and highlight ... | 1998 | 9639367 |
| effect of mild acid treatment on the survival, enteropathogenicity, and protein production in vibrio parahaemolyticus. | vibrio parahaemolyticus is an important food-borne enteropathogen that encounters various adverse conditions in its native environment or during infection. effects of mild acid treatment on survival under stress conditions, enteropathogenicity, and protein production in this pathogen were investigated. logarithmically grown cells, at ph 7.5 shifted to ph 5.0 for 30 min, were more resistant to subsequent acid challenge at ph 4.4. a two-phase adaptive procedure (ph 5.8 for 30 min; ph 5.0 for 30 mi ... | 1998 | 9632567 |
| opar, a homolog of vibrio harveyi luxr, controls opacity of vibrio parahaemolyticus. | vibrio parahaemolyticus is an organism well adapted to communal life on surfaces. when grown on a surface or in a viscous layer, the bacterium induces a large gene system and differentiates to swarmer cells capable of movement over and colonization of surfaces. v. parahaemolyticus displays additional phenotypic versatility manifested as variable colony morphology, switching between translucent and opaque colony types. although not itself luminescent, v. parahaemolyticus produces autoinducer mole ... | 1998 | 9620967 |