Publications

serological cross-reactions between acinetobacter calcoaceticus and chlamydiae.	a cross-reaction between acinetobacter calcoaceticus and chlamydiae is described. a water-soluble, heat stable, non-dialyzable antigen was extracted from acinetobacter species by boiling. this antigen fixed complement in the presence of homologous hyperimmune sera from rabbits or guinea pigs and in the presence of heterologous human or hyperimmunized animal sera containing chlamydial antibodies. hyperimmune antisera to the extracted antigen, or to suspensions of live acinetobacters, also reacted ...	1979	521480
glucose dehydrogenase from acinetobacter calcoaceticus: a 'quinoprotein'.		1979	520586
a hand infection from a. calcoaceticus (m. polymorpha).	acinetobacter calcoaceticus (mima polymorpha) is not a well known organism, and its identification can be confusing due to its variable morphology and sensitivity pattern. in a joint infection, delay in identification or appropriate treatment can result in added morbidity. an a. calcoaceticus (mima) infection in the hand seems not to have been previously reported. the present case involving a finger joint infection illustrates the pathogenicity of a. calcoaceticus (mima) and problems of treatmen ...	1979	477074
community-acquired acinetobacter pneumonia.	acinetobacter calcoaceticus var anitratus, a nonfermentative grampnegative bacillus, has been infrequently reported as a cause of community-acquired pneumonia. in this paper we describe the course of six recent patients with community-acquired, bacteremic pneumonia due to this organism and review the six previously reported cases. our experience suggests this organism is a more common cause of community-acquired pneumonia than previously thought. acinetobacter pneumonia occurs in older persons w ...	1979	463916
ventriculitis from acinetobacter calcoaceticus variant anitratus.		1979	448387
acinetobacter calcoaceticus: a nosocomial pathogen with an unusual seasonal pattern.		1979	448188
aminoglycoside-modifying enzymes among clinical isolates of acinetobacter calcoaceticus subsp. anitratus (herellea vaginicola): explanation for high-level aminoglycoside resistance.	acinetobacter calcoaceticus subsp. anitratus (herellea vaginicola) is an important cause of nosocomial infection in our hospital where a. calcoaceticus subsp. anitratus is the most frequently isolated gram-negative species resistant to one or more of the aminoglycoside antibiotics. of 167 strains tested for susceptibility to aminoglycosides, only 6 strains were found that were resistant to >/=128 mug of kanamycin per ml; all others were susceptible to </=32 mug/ml. five of these six strains were ...	1979	426512
acinetobacter calcoaceticus variety anitratus: an increasing nosocomial problem.	acinetobacter calcoaceticus var anitratus is an increasing nosocomial problem in some institutions. duirng a recent 16-month period, we observed 15 patients with blood isolates of this organism. thirteen of these patients acquired the organism from the hospital environment. eleven of these represented real disease and concurrent growth of the organism from another site was predictive of infection (p less than 0.03). a review of laboratory records showed that there was a three-fold increase in no ...	1979	426000
identification of gram-negative non-fermenters and oxidase-positive fermenters by the oxi/ferm tube.	since the recent introduction of the roche oxi/ferm tube to the uk two identification schemes have been developed by the manufacturer for use with the kit. we evaluated the success of these two schemes in identifying 222 predominantly culture collection strains belonging to 45 taxa of non-fermenters and nine taxa of oxidase-positive fermenters. the strains were chosen to represent all the taxa included in the two identification schemes developed by the manufacturer and we have therefore been abl ...	1979	372251
nosocomial respiratory tract infection and colonization with acinetobacter calcoaceticus. epidemiologic characteristics.	nosocomial respiratory tract infection with acinetobacter calcoaceticus occurs frequently in many hospitals. an outbreak of respiratory tract infections in an intensive care unit provided an opportunity to study clinical and epidemiologic characteristics of such infections. retrospective studies demonstrated that a. calcoaceticus in sputum was significantly associated with endotracheal intubation (p = 0.03) and continuous positive pressure ventilation (p less than 0.02). after control measures h ...	1978	362918
evaluation of the api 20e system for identification of nonfermentative gram-negative bacteria.	the api 20e system for enterobacteriaceae, recently broadened to include identification of nonfermentative gram-negative bacteria, was evaluated and compared with the conventional method for complete identification of 221 nonfermenters, which were well distributed into 48 species or biotypes and included organisms not listed in the api 20e data base. the results of 16 tests common to both systems were in close agreement. the api 20e system correctly identified 71 (43%) of the 165 organisms inclu ...	1978	353067
evaluation of the oxi/ferm tube system for identification of nonfermentative gram-negative bacilli.	the oxi/ferm tube system designed for identification of oxidative-fermentative gram-negative bacteria was evaluated for identification of nonfermentative gram-negative bacilli by comparing it with the conventional system. the nine biochemical reactions used as the initial tests in the oxi/ferm tube system showed a close agreement with the same conventional reactions. however, the system was only 41% accurate to species or biotype for complete identification of 239 isolates which were well distri ...	1978	353066
[acinetobacter calcoaceticus as the possible cause of bronchopneumonia in rabbits].		1978	352659
host factor for coliphage q beta rna replication: presence in procaryotes and association with the 30s ribosomal subunit in escherichia coli.	the host factor required for in vitro coliphage q beta rna replication, a heat-stable rna binding protein present in uninfected escherichia coli, has been detected by both immunological and functional tests in acinetobacter calcoaceticus, klebsiella pneumoniae, pseudomonas aeruginosa and pseudomonas putida. it was not detectable by these criteria in bacillus stearothermophilus, bacillus subtilis, caulobacter crescentus, micrococcus lysodeikticus, rhodopseudomonas capsulata or saccharomyces cerev ...	1977	329101
prosthetic valve endocarditis by opportunistic pathogens.	the incidence of endocarditis produced by the so-called "opportunists" as a complication of prosthetic valve surgery is progressively increasing in frequency and gradually transforming the clinical picture habitually associated with this disease. we report six cases of endocarditis produced by opportunistic microorganisms (two cases by candida, and the remaining by serratia, actinobacillus, acinetobacter calcoaceticus, and bacteroides fragilis, and by corynebacterium diphtheriae) in four male an ...	1977	319774
origins of metabolic diversity: evolutionary divergence by sequence repetition.	recurring patterns of primary structure have been observed in enzymes that mediate sequential metabolic reactions in bacteria. the enzymes, muconolactone delta-isomerase [(+)-4-hydroxy-4-carboxymethylisocrotonolactone delta(2)-delta(3)-isomerase, ec 5.3.3.4] and beta-ketoadipate enol-lactone hydrolase [4-carboxymethylbut-3-enolide(1,4)enol-lactone-hydrolase, ec 3.1.1.24], have been coselected in bacterial populations because the isomerase can confer no nutritional advantage in the absence of the ...	1979	291059
ecological effects of a deodorant and a plain soap upon human skin bacteria.	the effects of a commercial trichlorocarbanilide-containing deodorant soap and a commercial plain soap upon the cutaneous flora of individuals were compared. using a cross-over design, 21 volunteers (10 women and 11 men) washed their forearms at least once a day with one soap for 3 weeks and then switched soaps for another 4 weeks use. by analysis of variance no significant difference in total colony counts was noted among individuals in their use of the two soaps. with the exception of individu ...	1977	264493
outbreak of a multiply resistant acinetobacter in a surgical intensive care unit: epidemiology and control.	during january, 1976 seven patients in an sicu became colonized or infected with acinetobacter calcoaceticus (variation, anitratum) at an attack rate of 12.5 per cent. the organism showed a marked reduction in antimicrobial sensitivity from previous experience. comparison with 34 uninfected control patients indicated that intubation and continuous ventilatory assistance were significantly associated with acquiring the organism (p = 0.0154). acinetobacter was cultured from nurses' hands, ambu ada ...	1978	248372
the influence of changes in the environment on twitching motility.	by examining medium composition and cultural conditions quantitatively it was possible to define conditions suitable to bring twitching motility about. such conditions include the use of freshly poured, relatively thick and only slightly dried plates of a dilute medium in which the agar concentration is not too high. incubation should take place in a humid atmosphere. in fact, everything points to the humidity as a factor of the utmost importance. using strains of acinetobacter calcoaceticus, it ...	1975	239520
nadh and nadph-viologen reductases from acinetobacter calcoaceticus.	three pyridine nucleotide-dependent diaphorases have been isolated from acinetobacter calcoaceticus cells and partially characterized. two of them, with molecular weights of 165,000 and 57,000, utilize nadph as electron donor whereas the third one (mw = 57,000) is specific for nadh. oxidized viologen dyes, flavin nucleotides, dichlorophenol indophenol and ferricyanide can act with efficiency as acceptors in the reaction mediated by these diaphorases. the diaphorase activities have been character ...	1979	223203
[kinetic and regulatory properties of malate dehydrogenase from acinetobacter calcoaceticus].		1978	216197
phosphatase activity of aerobic and facultative anaerobic bacteria.	1115 strains of aerobic and facultatively anaerobic bacteria were tested for phosphatase activity by a conventional plate method and a microtest. the microtest was devised to allow results to be read after 4 h cultivation. phosphatase activity was found in wide range of species and strains. besides staphylococci, where the test for phosphatase is successfully used, it may be applied as one of the valuable tests for the differentiation of the following species: bacillus cereus, b. licheniformis, ...	1978	216188
regulation of synthesis of benzyl alcohol dehydrogenase in acinetobacter calcoaceticus ncib8250.	specific activity of benzyl alcohol dehydrogenase in carbon-limited continuous cultures was at a maximum at a specific growth rate of 0.2 h-1, but fell off at lower and higher growth rates. the specific activity in nitrogen-limited cultures was always lower and was inversely proportional to growth rate. there was severe repression of benzyl alcohol dehydrogenase during metabolism of l(+)-mandelate or phenylglyoxylate in batch cultures. synthesis of benzyl alcohol dehydrogenase was followed in ex ...	1977	201725
solubilization studies and properties of particulate nad-independent malate dehydrogenase of acinetobacter calcoaceticus (n.c.i.b. 8250).		1977	196960
in vitro and in vivo activities of minocycline and other antibiotics against acinetobacter (herellea-mima).	minocycline was the most active of six antibiotics tested against 65 clinical isolates of acinetobacter calcoaceticus (syn.: herellea, mima) received from six medical centers. in the bauer-kirby disk susceptibility test, all isolates were rated susceptible to minocycline, gentamicin, and polymyxin; 25% were resistant to tetracycline. in agar dilution tests, minocycline was two to four times more potent than gentamicin or polymyxin and eight times more potent than tetracycline. ampicillin and cep ...	1976	176936
the behaviour of nad+ and nadh in acinetobacter calcoaceticus during n-alkane assimilation.	the behaviour of the nicotinamide adenine dinucleotides nad+ and nadh in acinetobacter calcoaceticus during n-alkane assimilation was studied, acetate and succinate being used as reference carbon sources. the intracellular concentration of the two nucleotides was found to increase during the exponential growth phase, reaching its maximum in the phase of decreasing growth rates. in the exponential phase, the nad+/nadh quotients were less than 1 and showed only unimportant variations. in the phase ...	1975	170172
[infection by glucose-nonfermenting, gram-negative rods--infection by acinetobacter calcoaceticus].		1977	101606
microbial and chemical transformations of some 12,13-epoxytrichothec-9,10-enes.	resting cells of streptomyces griseus, mucor mucedo, and a growing culture of acinetobacter calcoaceticus when mixed with compounds related to 12,13-epoxytrichothec-9-ene-4beta,15-diacetoxy-3alpha-ol(anguidine) produced a series of derivatives that were either partially hydrolyzed or selectively acylated. these derivatives showed marked differences in activities as assayed by antifungal and tissue culture cytotoxicity tests.	1978	100053
studies on the oxidation of octane by acinetobacter calcoaceticus and flavobacterium devorans strains.		1979	94750
community-acquired acinetobacter calcoaceticus var anitratus pneumonia.	two patients had community-acquired acinetobacter calcoaceticus var anitratus pneumonia. both patients were alcoholic and one was cirrhotic. one patient died and the other received two weeks of gentamicin therapy and survived. misinterpretation of the sputum gram stain delayed diagnosis and institution of proper therapy in both cases. in addition to organisms sensitive to penicillins such as neisseria or haemophilus, acinetobacter must be considered in the differential diagnosis of community-acq ...	1977	70540
catechol 1,2-dioxygenase from acinetobacter calcoaceticus: purification and properties.	procedures for the purification of catechol 1,2-dioxygenase from extracts of acinetobacter calcoaceticus strain adp-96 are described. the purified enzyme was homogeneous as judged by ultracentrifugation and acrylamide gel electrophoresis. the enzyme contained 2 g-atoms of iron per mol of protein. the enzyme had a broad substrate specificity and catalyzed the oxidation of catechol, 4-methylcatechol, 3-methylcatechol, and 3-isopropyl catechol. the activity of the enzyme was inhibited by heavy meta ...	1976	58860
fine structure and distribution of extracellular polymer surrounding selected aerobic bacteria.	the structure and distribution of extracellular polymer surrounding bacillus circulans, diplococcus (streptococcus) pneumoniae, streptococcus salivarius, staphylococcus aureus, klebsiella pneumoniae, pseudomonas aeruginosa, herella vaginacola (acinetobacter calcoaceticus), and agrobacterium tumefaciens were studied by electron microscopy. a modified ruthenium red staining procedure was used to examine the fine structure of capsule and slime. freeze-etching and critical-point drying were used to ...	1975	46774
[properties of rubredoxin reductase from the alkane-assimilating bacterium acinetobacter calcoaceticus].		1979	44771
[oxidation of homologous aliphatic aldehydes by membrane-bound and solubilized aldehyde dehydrogenase from acinetobacter calcoaceticus].		1978	32669
purification and various properties of nadp+-dependent alcohol dehydrogenase from acinetobacter calcoaceticus.	the constitutive nadp+-dependent alcohol dehydrogenase from acinetobacter calcoaceticus can be accumulated about 50 fold in 3 purification steps. the end-product shows in the analytical polyacrylamide gel electrophoresis only one active enzyme band. the molecular weight of the enzyme was determined to be 235,000 by gel chromatography on sephadex g 200, the smallest subunit shows a molecular weight of 61 000 on sds electrophoresis. the isoelectric point is at 5.84. the km values determined with p ...	1976	14464
inhibition of the malic enzyme from acinetobacter calcoaceticus by nadph and nadh.	the malic enzyme enriched from acinetobacter calcoaceticus is inhibited by nadph and nadh. the inhibition afforded by the reduced coenzymes is not affected by nad+, amp and 3'.5'-amp. against l-malate, nadph inhibits the enzyme in a noncompetitive linear fashion (ki = 1.5 x 10(-4) m), against nadp+, competitively linearly (ki = 5.0 x 10(-5) m). while nadph acted as a product inhibitor, nadh seems to be an allosteric effector of the malic enzyme, because with l-malate as the variable substrate in ...	1975	9762
preparations and properties of ribonucleic acid polymerase from acinetobacter calcoaceticus.	deoxyribonucleic acid (dna)-dependent ribonucleic acid (rna) polymerase (ec 2.7.7.6) from acinetobacter calcoaceticus was purified to apparent homogeneity and its properties were compared with those of the escherichia coli b enzyme. the molecular weights of the two native active enzymes as well as their alpha and beta subunits appeared to be similar. no subunit corresponding to that of sigma from e. coli was found, and furthermore no separation between the beta subunits could be detected by gel ...	1976	1380
pyridine nucleotide-independent oxidation of long chain aliphatic alcohols by an enzyme of acinetobacter calcoaceticus.		1975	850
properties of the nadp+-dependent isocitrate dehydrogenases of acinetobacter calcoaceticus.		1975	849