Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| occurrence of an unusual hopanoid-containing lipid a among lipopolysaccharides from bradyrhizobium species. | the chemical structures of the unusual hopanoid-containing lipid a samples of the lipopolysaccharides (lps) from three strains of bradyrhizobium (slow-growing rhizobia) have been established. they differed considerably from other gram-negative bacteria in regards to the backbone structure, the number of ester-linked long chain hydroxylated fatty acids, as well as the presence of a tertiary residue that consisted of at least one molecule of carboxyl-bacteriohopanediol or its 2-methyl derivative. ... | 2014 | 25371196 |
| tyrosine 110 plays a critical role in regulating the allosteric inhibition of campylobacter jejuni dihydrodipicolinate synthase by lysine. | dihydrodipicolinate synthase (dhdps), an enzyme found in most bacteria and plants, controls a critical step in the biosynthesis of l-lysine and meso-diaminopimelate, necessary components for bacterial cell wall biosynthesis. dhdps catalyzes the condensation of pyruvate and (s)-aspartate-β-semialdehyde, forming an unstable product that is dehydrated to dihydrodipicolinate. the tetrameric enzyme is allosterically inhibited by l-lysine, and a better understanding of the allosteric inhibition mechan ... | 2014 | 25369463 |
| structural and mechanistic basis of zinc regulation across the e. coli zur regulon. | commensal microbes, whether they are beneficial or pathogenic, are sensitive to host processes that starve or swamp the prokaryote with large fluctuations in local zinc concentration. to understand how microorganisms coordinate a dynamic response to changes in zinc availability at the molecular level, we evaluated the molecular mechanism of the zinc-sensing zinc uptake regulator (zur) protein at each of the known zur-regulated genes in escherichia coli. we solved the structure of zinc-loaded zur ... | 2014 | 25369000 |
| crossroads between bacterial and mammalian glycosyltransferases. | bacterial glycosyltransferases (gt) often synthesize the same glycan linkages as mammalian gt; yet, they usually have very little sequence identity. nevertheless, enzymatic properties, folding, substrate specificities, and catalytic mechanisms of these enzyme proteins may have significant similarity. thus, bacterial gt can be utilized for the enzymatic synthesis of both bacterial and mammalian types of complex glycan structures. a comparison is made here between mammalian and bacterial enzymes t ... | 2014 | 25368613 |
| the biosynthesis of udp-d-fucnac-4n-(2)-oxoglutarate (udp-yelosamine) in bacillus cereus atcc 14579: pat and pyl, an aminotransferase and an atp-dependent grasp protein that ligates 2-oxoglutarate to udp-4-amino-sugars. | surface glycan switching is often observed when micro-organisms transition between different biotic and abiotic niches, including biofilms, although the advantages of this switching to the organism are not well understood. bacillus cereus grown in a biofilm-inducing medium has been shown to synthesize an unusual cell wall polysaccharide composed of the repeating subunit →6)gal(α1-2)(2-r-hydroxyglutar-5-ylamido)fuc2nac4n(α1-6)glcnac(β1→, where galactose is linked to the hydroxyglutarate moiety of ... | 2014 | 25368324 |
| interoceptive dysfunction: toward an integrated framework for understanding somatic and affective disturbance in depression. | depression is characterized by disturbed sleep and eating, a variety of other nonspecific somatic symptoms, and significant somatic comorbidities. why there is such close association between cognitive and somatic dysfunction in depression is nonetheless poorly understood. an explosion of research in the area of interoception-the perception and interpretation of bodily signals-over the last decade nonetheless holds promise for illuminating what have until now been obscure links between the social ... | 2014 | 25365763 |
| interoceptive dysfunction: toward an integrated framework for understanding somatic and affective disturbance in depression. | depression is characterized by disturbed sleep and eating, a variety of other nonspecific somatic symptoms, and significant somatic comorbidities. why there is such close association between cognitive and somatic dysfunction in depression is nonetheless poorly understood. an explosion of research in the area of interoception-the perception and interpretation of bodily signals-over the last decade nonetheless holds promise for illuminating what have until now been obscure links between the social ... | 2014 | 25365763 |
| prevalence of salmonella and campylobacter on broiler chickens from farm to slaughter and efficiency of methods to remove visible fecal contamination. | a study was conducted to investigate the prevalence of salmonella and campylobacter from farm to slaughter. the efficiency of trimming and water spray (490 to 588 kpa pressure) on the removal of visible fecal contamination from broiler carcasses before chilling was also investigated. drag swabs were used to sample litter from the farm houses. samples of ceca and carcasses without and with visible fecal contamination before and after trimming or spray washing of fecal contamination were taken dur ... | 2014 | 25364917 |
| bacterial etiologies of five core syndromes: laboratory-based syndromic surveillance conducted in guangxi, china. | under the existing national surveillance system in china for selected infectious diseases, bacterial cultures are performed for only a small percentage of reported cases. we set up a laboratory-based syndromic surveillance system to elucidate bacterial etiologic spectrum and detect infection by rare etiologies (or serogroups) for five core syndromes in the given study area. | 2014 | 25360596 |
| peripheral nervous system manifestations of infectious diseases. | infectious causes of peripheral nervous system (pns) disease are underrecognized but potentially treatable. heightened awareness educed by advanced understanding of the presentations and management of these infections can aid diagnosis and facilitate treatment. in this review, we discuss the clinical manifestations, diagnosis, and treatment of common bacterial, viral, and parasitic infections that affect the pns. we additionally detail pns side effects of some frequently used antimicrobial agent ... | 2014 | 25360209 |
| guillain-barré syndrome and severe infection following chemotherapy for peripheral t-cell lymphoma: a case report. | guillain-barré syndrome (gbs) is a rare complication of malignant lymphoma. the current study describes a case of gbs in a patient with peripheral t-cell lymphoma not otherwise specified (ptcl-nos). a 47-year-old male was admitted to the first affiliated hospital of zhengzhou university (zhengzhou, china) with systemic multiple subcutaneous nodules and was diagnosed with stage iv high-grade ptcl-nos (according to the revised european american lymphoma classification). during chemotherapy, severe ... | 2014 | 25360176 |
| composition of diet modifies colonization dynamics of campylobacter jejuni in broiler chickens. | to evaluate the impact of diet composition on colonization dynamics of camp. jejuni and on related physiological parameters in the chicken intestine. | 2015 | 25358748 |
| a severe case of persistent diarrhoea associated with arcobacter cryaerophilus but attributed to campylobacter sp. and a review of the clinical incidence of arcobacter spp. | although rarely, arcobacter spp. have been associated with diarrhoea and bacteraemia. we report a persistent case in a healthy 26-year-old spanish male of bloody diarrhoea, which was attributed to campylobacter but in fact was caused by arcobacter cryaerophilus, as determined by sequencing of the rpob gene. the isolate was re-identified by matrix-assisted laser desorption ionization time of flight (maldi-tof) and genotyped for five putative virulence genes and for seven genes included in the arc ... | 2014 | 25356338 |
| intestinal microbiota pathogenesis and fecal microbiota transplantation for inflammatory bowel disease. | the intestinal microbiota plays an important role in inflammatory bowel disease (ibd). the pathogenesis of ibd involves inappropriate ongoing activation of the mucosal immune system driven by abnormal intestinal microbiota in genetically predisposed individuals. however, there are still no definitive microbial pathogens linked to the onset of ibd. the composition and function of the intestinal microbiota and their metabolites are indeed disturbed in ibd patients. the special alterations of gut m ... | 2014 | 25356041 |
| evidence-based assessment of proton-pump inhibitors in helicobacter pylori eradication: a systematic review. | peptic ulcer disease continues to be issue especially due to its high prevalence in the developing world. helicobacter pylori (h. pylori) infection associated duodenal ulcers should undergo eradication therapy. there are many regimens offered for h. pylori eradication which include triple, quadruple, or sequential therapy regimens. the central aim of this systematic review is to evaluate the evidence for h. pylori therapy from a meta-analytical outlook. the consequence of the dose, type of proto ... | 2014 | 25356018 |
| a receptor-binding protein of campylobacter jejuni bacteriophage nctc 12673 recognizes flagellin glycosylated with acetamidino-modified pseudaminic acid. | bacteriophage receptor-binding proteins (rbps) confer host specificity. we previously identified a putative rbp (gp047) from the campylobacter lytic phage nctc 12673 and demonstrated that gp047 has a broader host range than its parent phage. while nctc 12673 recognizes the capsular polysaccharide (cps) of a limited number of campylobacter jejuni isolates, gp047 binds to a majority of c. jejuni and related campylobacter coli strains. in this study, we demonstrate that gp047 also binds to acapsula ... | 2015 | 25354466 |
| antimicrobial proteins in intestine and inflammatory bowel diseases. | mucosal surface of the intestinal tract is continuously exposed to a large number of microorganisms. to manage the substantial microbial exposure, epithelial surfaces produce a diverse arsenal of antimicrobial proteins (amps) that directly kill or inhibit the growth of microorganisms. thus, amps are important components of innate immunity in the gut mucosa. they are frequently expressed in response to colonic inflammation and infection. expression of many amps, including human β-defensin 2-4 and ... | 2014 | 25349560 |
| systematic review with meta-analysis: post-infectious functional dyspepsia. | the prevalence of functional dyspepsia (fd) following infectious gastroenteritis has not been systematically reviewed. | 2015 | 25348873 |
| differences in carbon source utilisation distinguish campylobacter jejuni from campylobacter coli. | campylobacter jejuni and c. coli are human intestinal pathogens that are the most frequent causes of bacterial foodborne gastroenteritis in humans in the uk. in this study, we aimed to characterise the metabolic diversity of both c. jejuni and c. coli using a diverse panel of clinical strains isolated from the uk, pakistan and thailand, thereby representing both the developed and developing world. our aim was to apply multi genome analysis and biolog phenotyping to determine differences in carbo ... | 2014 | 25348335 |
| salmonella enterica in the chicken: how it has helped our understanding of immunology in a non-biomedical model species. | salmonella infection of the chicken is important both as a source of foodborne human salmonellosis and as a source of disease in the chicken itself. vaccination and other control strategies require an understanding of the immune response and as such have been important in understanding both mucosal immunity and more generally the response to bacterial infection. in this review, we discuss the contribution the study of avian salmonellosis has made to understanding innate immunity including the fu ... | 2014 | 25346731 |
| [preparation and characterization of monoclonal antibodies against cytolethal distending toxin protein of campylobacter jejuni]. | to express campylobacter jejuni cytolethal distending toxin b protein (cdtb) in a prokaryote to prepare monoclonal antibodies (mabs) against the protein, and to study their antitoxic effects. | 2014 | 25345028 |
| identification of a virulence-related surface protein xf in piscine streptococcus agalactiae by pre-absorbed immunoproteomics. | since 2009, large-scale streptococcus agalactiae infections have broken out in cultured tilapia farms in china, resulting in considerable economic losses. screening of the surface proteins is required to identify virulence factors or protective antigens involved in piscine s.agalactiae infections in tilapia. pre-absorbed immunoproteomics method (paim) is a useful method previously established in our laboratory for identifying bacterial surface proteins. | 2014 | 25344337 |
| emerging rapid resistance testing methods for clinical microbiology laboratories and their potential impact on patient management. | atypical and multidrug resistance, especially esbl and carbapenemase expressing enterobacteriaceae, is globally spreading. therefore, it becomes increasingly difficult to achieve therapeutic success by calculated antibiotic therapy. consequently, rapid antibiotic resistance testing is essential. various molecular and mass spectrometry-based approaches have been introduced in diagnostic microbiology to speed up the providing of reliable resistance data. pcr- and sequencing-based approaches are th ... | 2014 | 25343142 |
| the genome of the intracellular bacterium of the coastal bivalve, solemya velum: a blueprint for thriving in and out of symbiosis. | symbioses between chemoautotrophic bacteria and marine invertebrates are rare examples of living systems that are virtually independent of photosynthetic primary production. these associations have evolved multiple times in marine habitats, such as deep-sea hydrothermal vents and reducing sediments, characterized by steep gradients of oxygen and reduced chemicals. due to difficulties associated with maintaining these symbioses in the laboratory and culturing the symbiotic bacteria, studies of ch ... | 2014 | 25342549 |
| human viruses and cancer. | the first human tumor virus was discovered in the middle of the last century by anthony epstein, bert achong and yvonne barr in african pediatric patients with burkitt's lymphoma. to date, seven viruses -ebv, kshv, high-risk hpv, mcpv, hbv, hcv and htlv1- have been consistently linked to different types of human cancer, and infections are estimated to account for up to 20% of all cancer cases worldwide. viral oncogenic mechanisms generally include: generation of genomic instability, increase in ... | 2014 | 25341666 |
| uneven genetic robustness of hiv-1 integrase. | genetic robustness (tolerance of mutation) may be a naturally selected property in some viruses, because it should enhance adaptability. robustness should be especially beneficial to viruses like hiv-1 that exhibit high mutation rates and exist in immunologically hostile environments. surprisingly, however, the hiv-1 capsid protein (ca) exhibits extreme fragility. to determine whether fragility is a general property of hiv-1 proteins, we created a large library of random, single-amino-acid mutan ... | 2014 | 25339768 |
| uneven genetic robustness of hiv-1 integrase. | genetic robustness (tolerance of mutation) may be a naturally selected property in some viruses, because it should enhance adaptability. robustness should be especially beneficial to viruses like hiv-1 that exhibit high mutation rates and exist in immunologically hostile environments. surprisingly, however, the hiv-1 capsid protein (ca) exhibits extreme fragility. to determine whether fragility is a general property of hiv-1 proteins, we created a large library of random, single-amino-acid mutan ... | 2014 | 25339768 |
| identification of antigenic proteins of the nosocomial pathogen klebsiella pneumoniae. | the continuous expansion of nosocomial infections around the globe has become a precarious situation. key challenges include mounting dissemination of multiple resistances to antibiotics, the easy transmission and the growing mortality rates of hospital-acquired bacterial diseases. thus, new ways to rapidly detect these infections are vital. consequently, researchers around the globe pursue innovative approaches for point-of-care devices. in many cases the specific interaction of an antigen and ... | 2014 | 25333280 |
| characterization of the genetic environment of the ribosomal rna methylase gene erm(b) in campylobacter jejuni. | 2015 | 25331057 | |
| flooding and emergency room visits for gastrointestinal illness in massachusetts: a case-crossover study. | floods and other severe weather events are anticipated to increase as a result of global climate change. floods can lead to outbreaks of gastroenteritis and other infectious diseases due to disruption of sewage and water infrastructure and impacts on sanitation and hygiene. floods have also been indirectly associated with outbreaks through population displacement and crowding. | 2014 | 25329916 |
| bioluminescence atp monitoring for the routine assessment of food contact surface cleanliness in a university canteen. | atp bioluminescence monitoring and traditional microbiological analyses (viable counting of total mesophilic aerobes, coliforms and escherichia coli) were used to evaluate the effectiveness of sanitation standard operating procedures (ssop) at a university canteen which uses a haccp-based approach. to that end, 10 cleaning control points (cps), including food contact surfaces at risk of contamination from product residues or microbial growth, were analysed during an 8-month monitoring period. ar ... | 2014 | 25329534 |
| evaluation of a real-time multiplex pcr for the simultaneous detection of campylobacter jejuni, salmonella spp., shigella spp./eiec, and yersinia enterocolitica in fecal samples. | conventional diagnosis of infectious diarrhea caused by bacteria is time-consuming, labor-intensive, and has a suboptimal sensitivity. we have therefore developed a multiplex real-time polymerase chain reaction (pcr) for the simultaneous detection of campylobacter jejuni, salmonella spp., shigella spp./enteroinvasive escherichia coli (eiec), and yersinia enterocolitica in fecal samples. no cross reactivity between the different pathogens was observed, and the multiplex setup of the assay did not ... | 2015 | 25326870 |
| epidemiology of human parechovirus, aichi virus and salivirus in fecal samples from hospitalized children with gastroenteritis in hong kong. | emerging human picornaviruses, including human parechovirus (hpev), aichi virus (aiv) and salivirus (salv) were found to be associated with gastroenteritis, but their roles in enteric infections are not fully understood. in addition, no report on the circulation of these viruses in hong kong is available. the objective of this study was to investigate the prevalence and genetic diversity of hpev, aiv and salv in fecal samples from hospitalized children with gastroenteritis in hong kong. | 2014 | 25326707 |
| siderophore-mediated iron acquisition influences motility and is required for full virulence of the xylem-dwelling bacterial phytopathogen pantoea stewartii subsp. stewartii. | iron is a key micronutrient for microbial growth but is often present in low concentrations or in biologically unavailable forms. many microorganisms overcome this challenge by producing siderophores, which are ferric-iron chelating compounds that enable the solubilization and acquisition of iron in a bioactive form. pantoea stewartii subsp. stewartii, the causal agent of stewart's wilt of sweet corn, produces a siderophore under iron-limiting conditions. the proteins involved in the biosynthesi ... | 2014 | 25326304 |
| siderophore-mediated iron acquisition influences motility and is required for full virulence of the xylem-dwelling bacterial phytopathogen pantoea stewartii subsp. stewartii. | iron is a key micronutrient for microbial growth but is often present in low concentrations or in biologically unavailable forms. many microorganisms overcome this challenge by producing siderophores, which are ferric-iron chelating compounds that enable the solubilization and acquisition of iron in a bioactive form. pantoea stewartii subsp. stewartii, the causal agent of stewart's wilt of sweet corn, produces a siderophore under iron-limiting conditions. the proteins involved in the biosynthesi ... | 2014 | 25326304 |
| identification of ypqp as a new bacillus subtilis biofilm determinant that mediates the protection of staphylococcus aureus against antimicrobial agents in mixed-species communities. | in most habitats, microbial life is organized in biofilms, three-dimensional edifices sustained by extracellular polymeric substances that enable bacteria to resist harsh and changing environments. under multispecies conditions, bacteria can benefit from the polymers produced by other species ("public goods"), thus improving their survival under toxic conditions. a recent study showed that a bacillus subtilis hospital isolate (ndmed) was able to protect staphylococcus aureus from biocide action ... | 2014 | 25326298 |
| identification of ypqp as a new bacillus subtilis biofilm determinant that mediates the protection of staphylococcus aureus against antimicrobial agents in mixed-species communities. | in most habitats, microbial life is organized in biofilms, three-dimensional edifices sustained by extracellular polymeric substances that enable bacteria to resist harsh and changing environments. under multispecies conditions, bacteria can benefit from the polymers produced by other species ("public goods"), thus improving their survival under toxic conditions. a recent study showed that a bacillus subtilis hospital isolate (ndmed) was able to protect staphylococcus aureus from biocide action ... | 2014 | 25326298 |
| defining the metabolic requirements for the growth and colonization capacity of campylobacter jejuni. | during the last decade campylobacter jejuni has been recognized as the leading cause of bacterial gastroenteritis worldwide. this facultative intracellular pathogen is a member of the epsilonproteobacteria and requires microaerobic atmosphere and nutrient rich media for efficient proliferation in vitro. its catabolic capacity is highly restricted in contrast to salmonella typhimurium and other enteropathogenic bacteria because several common pathways for carbohydrate utilization are either missi ... | 2014 | 25325018 |
| inflammation-related carcinogenesis: current findings in epidemiological trends, causes and mechanisms. | inflammation is a definite cancer-causing factor as revealed by cumulative basic, clinical and epidemiological studies. it is mostly induced by infectious agents. for instance, infection with papillomaviruses associates with anogenital cancers, especially cervical cancers; helicobacter pylori infection of the stomach tends to increase the risk of stomach cancer; chronic hepatitis b & c viruses and fluke infections of the liver increase liver cancers; autoimmune diseases, e.g., inflammatory bowel ... | 2014 | 25324587 |
| an assessment of risk posed by a campylobacter-positive puppy living in an australian residential aged-care facility. | in april and june 2012, two outbreaks of campylobacter gastroenteritis were investigated in an australian aged-care facility (acf); a campylobacter-positive puppy was identified as a potential source of infection. | 2014 | 25320673 |
| purification and characterization of bacteriocin produced by lactobacillus brevis un isolated from dhulliachar: a traditional food product of north east india. | a bacteriocin producing strain lactobacillus brevis un isolated from dulliachar-a salted pickle and identified by biochemical and molecular methods. l. brevis un was found to produce bacteriocin with broad spectrum activity against spoilage causing/food borne pathogens viz. l. monocytogenes, c. perfringens, s. aureus, l. mesenteroides, l. plantarum and b. cereus. bacteriocin production was optimized through classical one variable at a time method. the isolate showed maximum bacteriocin productio ... | 2013 | 25320420 |
| purification and characterization of bacteriocin produced by lactobacillus brevis un isolated from dhulliachar: a traditional food product of north east india. | a bacteriocin producing strain lactobacillus brevis un isolated from dulliachar-a salted pickle and identified by biochemical and molecular methods. l. brevis un was found to produce bacteriocin with broad spectrum activity against spoilage causing/food borne pathogens viz. l. monocytogenes, c. perfringens, s. aureus, l. mesenteroides, l. plantarum and b. cereus. bacteriocin production was optimized through classical one variable at a time method. the isolate showed maximum bacteriocin productio ... | 2013 | 25320420 |
| hybrid alkali-hydrodynamic disintegration of waste-activated sludge before two-stage anaerobic digestion process. | the first step of anaerobic digestion, the hydrolysis, is regarded as the rate-limiting step in the degradation of complex organic compounds, such as waste-activated sludge (was). the aim of lab-scale experiments was to pre-hydrolyze the sludge by means of low intensive alkaline sludge conditioning before applying hydrodynamic disintegration, as the pre-treatment procedure. application of both processes as a hybrid disintegration sludge technology resulted in a higher organic matter release (sol ... | 2014 | 25318422 |
| hybrid alkali-hydrodynamic disintegration of waste-activated sludge before two-stage anaerobic digestion process. | the first step of anaerobic digestion, the hydrolysis, is regarded as the rate-limiting step in the degradation of complex organic compounds, such as waste-activated sludge (was). the aim of lab-scale experiments was to pre-hydrolyze the sludge by means of low intensive alkaline sludge conditioning before applying hydrodynamic disintegration, as the pre-treatment procedure. application of both processes as a hybrid disintegration sludge technology resulted in a higher organic matter release (sol ... | 2014 | 25318422 |
| detection of zoonotic pathogens and characterization of novel viruses carried by commensal rattus norvegicus in new york city. | norway rats (rattus norvegicus) are globally distributed and concentrate in urban environments, where they live and feed in closer proximity to human populations than most other mammals. despite the potential role of rats as reservoirs of zoonotic diseases, the microbial diversity present in urban rat populations remains unexplored. in this study, we used targeted molecular assays to detect known bacterial, viral, and protozoan human pathogens and unbiased high-throughput sequencing to identify ... | 2014 | 25316698 |
| unacylated tridecaptin a₁ acts as an effective sensitiser of gram-negative bacteria to other antibiotics. | a derivative of the linear cationic lipopeptide tridecaptin a₁missing the n-terminal lipophilic acyl group, termed h-tria₁, is devoid of antimicrobial activity but is extremely effective at sensitising gram-negative bacteria to certain antibiotics. h-tria₁has low cytotoxicity compared with the natural peptide and in low concentrations it can substantially lower the minimum inhibitory concentration (mic) of some antibiotics against strains of escherichia coli, campylobacter jejuni and klebsiella ... | 2014 | 25315408 |
| a structural mechanism for bacterial autotransporter glycosylation by a dodecameric heptosyltransferase family. | a large group of bacterial virulence autotransporters including aida-i from diffusely adhering e. coli (daec) and tiba from enterotoxigenic e. coli (etec) require hyperglycosylation for functioning. here we demonstrate that tibc from etec harbors a heptosyltransferase activity on tiba and aida-i, defining a large family of bacterial autotransporter heptosyltransferases (bahts). the crystal structure of tibc reveals a characteristic ring-shape dodecamer. the protomer features an n-terminal β-barr ... | 2014 | 25310236 |
| mode of action of lactoperoxidase as related to its antimicrobial activity: a review. | lactoperoxidase is a member of the family of the mammalian heme peroxidases which have a broad spectrum of activity. their best known effect is their antimicrobial activity that arouses much interest in in vivo and in vitro applications. in this context, the proper use of lactoperoxidase needs a good understanding of its mode of action, of the factors that favor or limit its activity, and of the features and properties of the active molecules. the first part of this review describes briefly the ... | 2014 | 25309750 |
| the amazing transglycosylation activity of endo-β-n-acetylglucosaminidases. | major advances have been made in exploring the transglycosylation activity of endo-β-n-acetylglucosaminidases (engases) for synthetic purpose. the exploration of synthetic sugar oxazolines as donor substrates for the engase-catalyzed transglycosylation has expanded the substrate availability and significantly enhanced the overall transglycosylation efficiency. on the other hand, site-directed mutagenesis in combination with activity screening has led to the discovery of the first generation enga ... | 2011 | 25309039 |
| interaction between campylobacter and intestinal epithelial cells leads to a different proinflammatory response in human and porcine host. | campylobacter jejuni and campylobacter coli are recognized as the leading causes of human diarrheal disease throughout the development world. unlike human beings, gastrointestinal tract of pigs are frequently colonized by campylobacter to a high level in a commensal manner. the aim of this study was to identify the differences underlying the divergent outcome following campylobacter challenge in porcine versus human host. in order to address this, a comparative in vitro infection model was combi ... | 2014 | 25307769 |
| the as87_04050 gene is involved in bacterial lipopolysaccharide biosynthesis and pathogenicity of riemerella anatipestifer. | riemerella anatipestifer is reported worldwide as a cause of septicemic and exudative diseases of domestic ducks. in this study, we identified a mutant strain ra2640 by tn4351 transposon mutagenesis, in which the as87_04050 gene was inactivated by insertion of the transposon. southern blot analysis indicated that only one insertion was found in the genome of the mutant strain ra2640. sds-page followed by silver staining showed that the lipopolysaccharide (lps) pattern of mutant strain ra2640 was ... | 2014 | 25303276 |
| developmentally-regulated excision of the spβ prophage reconstitutes a gene required for spore envelope maturation in bacillus subtilis. | temperate phages infect bacteria by injecting their dna into bacterial cells, where it becomes incorporated into the host genome as a prophage. in the genome of bacillus subtilis 168, an active prophage, spβ, is inserted into a polysaccharide synthesis gene, spsm. here, we show that a rearrangement occurs during sporulation to reconstitute a functional composite spsm gene by precise excision of spβ from the chromosome. spβ excision requires a putative site-specific recombinase, spra, and an acce ... | 2014 | 25299644 |
| bacteremia due to campylobacter in renal transplantation: a case report and review of literature. | campylobacter species are the leading cause of acute bacterial diarrhea in industrialized countries. however, bacteremia is detected in <1% of patients with campylobacter enteritis and is most likely to occur in patients who are immunocompromised or of older age. to our knowledge, only 2 cases of campylobacter jejuni bacteremia have been reported in renal transplant recipients (rtrs). we present a case of an rtr with c. jejuni bacteremia presenting as self-limiting diarrhea followed by fever and ... | 2014 | 25296529 |
| outbreaks of acute gastroenteritis transmitted by person-to-person contact--united states, 2009-2010. | 2014 | 25295609 | |
| [thrombotic thrombocytopenic purpura in a child with low adamts13 enzyme level]. | thrombotic thrombocytopenic purpura (ttp) is a rare condition, but important to consider in case of thrombocytopenia and haemolysis. it is imperative to proceed with the correct treatment, in order to ensure a satisfactory outcome. ttp is either acquired or idiopathic. this case report shows that a 14-year-old boy has acquired ttp due to an infection with campylobacter jejuni and samonella szentes. plasma exchange plays an essential role in the treatment of ttp. | 2014 | 25293573 |
| bacterial clpb heat-shock protein, an antigen-mimetic of the anorexigenic peptide α-msh, at the origin of eating disorders. | the molecular mechanisms at the origin of eating disorders (eds), including anorexia nervosa (an), bulimia and binge-eating disorder (bed), are currently unknown. previous data indicated that immunoglobulins (igs) or autoantibodies (auto-abs) reactive with α-melanocyte-stimulating hormone (α-msh) are involved in regulation of feeding and emotion; however, the origin of such auto-abs is unknown. here, using proteomics, we identified clpb heat-shock disaggregation chaperone protein of commensal gu ... | 2014 | 25290265 |
| the interaction of human enteric pathogens with plants. | there are an increasing number of outbreaks of human pathogens related to fresh produce. thus, the growth of human pathogens on plants should be explored. human pathogens can survive under the harsh environments in plants, and can adhere and actively invade plants. plant-associated microbiota or insects contribute to the survival and transmission of enteric pathogens in plants. human enteric pathogens also trigger plant innate immunity, but some pathogens-such as salmonella-can overcome this def ... | 2014 | 25288993 |
| production, crystallization and preliminary crystallographic analysis of allochromatium vinosum thiosulfate dehydrogenase tsda, an unusual acidophilic c-type cytochrome. | the ability to perform the very simple oxidation of two molecules of thiosulfate to tetrathionate is widespread among prokaryotes. despite the prevalent occurrence of tetrathionate formation and its well documented significance within the sulfur cycle, little is known about the enzymes that catalyze the oxidative condensation of two thiosulfate anions. to fill this gap, the thiosulfate dehydrogenase (tsda) enzyme from the purple sulfur bacterium allochromatium vinosum was recombinantly expressed ... | 2014 | 25286955 |
| crystallization and preliminary x-ray diffraction analysis of cyclolavandulyl diphosphate synthase, a new member of the cis-isoprenyl diphosphate synthase superfamily. | cyclolavandulyl diphosphate synthase (clds; estimated molecular weight 23.1 kda) from the soil bacterium streptomyces sp. cl190 is an enzyme that catalyzes both the condensation of two molecules of c5 dimethylallyl diphosphate (dmapp) and the subsequent cyclization. clds was crystallized in the absence and the presence of the substrate dmapp. diffraction data were collected at a synchrotron source and the crystals diffracted to 2.00 and 1.73 å resolution, respectively. the crystal obtained in th ... | 2014 | 25286951 |
| crystallographic study of the phosphoethanolamine transferase eptc required for polymyxin resistance and motility in campylobacter jejuni. | the foodborne enteric pathogen campylobacter jejuni decorates a variety of its cell-surface structures with phosphoethanolamine (petn). modifying lipid a with petn promotes cationic antimicrobial peptide resistance, whereas post-translationally modifying the flagellar rod protein flgg with petn promotes flagellar assembly and motility, which are processes that are important for intestinal colonization. eptc, the petn transferase required for all known petn cell-surface modifications in c. jejuni ... | 2014 | 25286856 |
| method-dependent variability in determination of prevalence of campylobacter jejuni and campylobacter coli in canadian retail poultry. | campylobacter is the most frequent cause of bacterial gastroenteritis in canada, and the illness is commonly associated with poultry consumption. whereas canadian retail poultry is often contaminated with campylobacters, studies on the prevalence of this organism are inconsistent due to variability in sampling and microbiological methodology. to determine the current microbiological status of canadian poultry, and to evaluate two commonly used microbiological methods, 348 raw poultry samples wer ... | 2014 | 25285484 |
| selective microbial genomic dna isolation using restriction endonucleases. | to improve the metagenomic analysis of complex microbiomes, we have repurposed restriction endonucleases as methyl specific dna binding proteins. as an example, we use dpni immobilized on magnetic beads. the ten minute extraction technique allows specific binding of genomes containing the dpni gm6atc motif common in the genomic dna of many bacteria including γ-proteobacteria. using synthetic genome mixtures, we demonstrate 80% recovery of escherichia coli genomic dna even when only femtogram qua ... | 2014 | 25279840 |
| endoscopic features of gastro-intestinal lymphomas: from diagnosis to follow-up. | many progresses have been done in the management of gastrointestinal (gi) lymphomas during last decades, especially after the discovery of helicobacter pylori-dependent lymphoma development. the stepwise implementation of new endoscopic techniques, by means of echoendoscopy or double-balloon enteroscopy, enabled us to more precisely describe the endoscopic features of gi lymphomas with substantial contribution in patient management and in tailoring the treatment strategy with organ preserving ap ... | 2014 | 25278693 |
| beyond the stomach: an updated view of helicobacter pylori pathogenesis, diagnosis, and treatment. | helicobacter pylori (h. pylori) is an extremely common, yet underappreciated, pathogen that is able to alter host physiology and subvert the host immune response, allowing it to persist for the life of the host. h. pylori is the primary cause of peptic ulcers and gastric cancer. in the united states, the annual cost associated with peptic ulcer disease is estimated to be $6 billion and gastric cancer kills over 700000 people per year globally. the prevalence of h. pylori infection remains high ( ... | 2014 | 25278678 |
| emerging and reemerging neglected tropical diseases: a review of key characteristics, risk factors, and the policy and innovation environment. | in global health, critical challenges have arisen from infectious diseases, including the emergence and reemergence of old and new infectious diseases. emergence and reemergence are accelerated by rapid human development, including numerous changes in demographics, populations, and the environment. this has also led to zoonoses in the changing human-animal ecosystem, which are impacted by a growing globalized society where pathogens do not recognize geopolitical borders. within this context, neg ... | 2014 | 25278579 |
| microbiological analysis of environmental samples collected from child care facilities in north and south carolina. | children cared for outside the home are at an increased risk of enteric disease. microbiological analyses were performed on environmental samples collected from child care facilities in north and south carolina. | 2014 | 25278392 |
| association between human leukocyte antigen-dr and demylinating guillain-barre syndrome. | to find an association between human leukocyte antigen (hla) class ii drb1, drb3, drb4, and drb5 alleles frequencies in a sample of iraqi patients with guillain-barre syndrome (gbs) and compare with a healthy control group. | 2014 | 25274590 |
| stress response and virulence of heat-stressed campylobacter jejuni. | thermotolerant campylobacter spp. frequently cause bacterial gastroenteritis in humans commonly infected through the consumption of undercooked poultry meat. we examined campylobacter jejuni heat-stress responses in vitro after exposure to 48°c and 55°c. the in vivo modulation of its pathogenicity was also investigated using balb/c mice intravenously infected with stressed c. jejuni. regardless of the bacterial growth phase, the culturability and viability of c. jejuni in vitro was reduced after ... | 2014 | 25273228 |
| natural control of bacteria affecting meat quality by a neem (azadirachta indica a. juss) cake extract. | the antibacterial activity of an ethylacetate neem cake extract (nce) against bacteria that affect meat quality, namely campylobacter jejuni, carnobacterium spp., lactobacillus curvatus, lactobacillus sakei and leuconostoc sp., is reported. the antibacterial activity was detected using standardised disc diffusion and macrodilution methods. the bacterial growth inhibition zone ranged from 11.33 ± 0.58 to 22.67 ± 0.58 mm (100 μl nce). there is significant difference between the growth inhibition z ... | 2015 | 25272067 |
| prevalence of arcobacter species among humans, belgium, 2008-2013. | we examined fecal samples from 6,774 patients with enteritis in belgium, 2008-2013. members of the genus arcobacter were the fourth most common pathogen group isolated, and the isolation rate was higher than previously reported. culturing arcobacter in a microbiology laboratory is feasible and should thus be tested for in cases of diarrheal disease. | 2014 | 25271569 |
| cell factories for insulin production. | the rapid increase in the number of diabetic patients globally and exploration of alternate insulin delivery methods such as inhalation or oral route that rely on higher doses, is bound to escalate the demand for recombinant insulin in near future. current manufacturing technologies would be unable to meet the growing demand of affordable insulin due to limitation in production capacity and high production cost. manufacturing of therapeutic recombinant proteins require an appropriate host organi ... | 2014 | 25270715 |
| detection and characterization of cancer cells and pathogenic bacteria using aptamer-based nano-conjugates. | detection and characterization of cells using aptamers and aptamer-conjugated nanoprobes has evolved a great deal over the past few decades. this evolution has been driven by the easy selection of aptamers via in vitro cell-selex, permitting sensitive discrimination between target and normal cells, which includes pathogenic prokaryotic and cancerous eukaryotic cells. additionally, when the aptamer-based strategies are used in conjunction with nanomaterials, there is the potential for cell target ... | 2014 | 25268922 |
| formate dehydrogenase localization and activity are dependent on an intact twin arginine translocation system (tat) in campylobacter jejuni 81-176. | the oxidation of formate constitutes a main energy source for campylobacter jejuni, an important foodborne pathogen. formate occurs in the hosts' gut as a byproduct of fermentation, which may contribute to c. jejuni's adaptation to that niche. c. jejuni possesses a periplasmic formate dehydrogenase (fdh) that facilitates the metabolism of formate. in c. jejuni nctc-11168, a poorly invasive strain, fdh localizes to the periplasm via the twin arginine translocation (tat) system, and the fdha encod ... | 2014 | 25268895 |
| inflammasome activation by campylobacter jejuni. | the gram-negative pathogen campylobacter jejuni is the most common cause of bacterial foodborne disease worldwide. the mechanisms that lead to bacterial invasion of eukaryotic cells and massive intestinal inflammation are still unknown. in this study, we report that c. jejuni infection of mouse macrophages induces upregulation of pro-il-1β transcript and secretion of il-1β without eliciting cell death. immunoblotting indicated cleavage of caspase-1 and il-1β in infected cells. in bone marrow-der ... | 2014 | 25267974 |
| bordetella parapertussis survives inside human macrophages in lipid raft-enriched phagosomes. | bordetella parapertussis is a human pathogen that causes whooping cough. the increasing incidence of b. parapertussis has been attributed to the lack of cross protection induced by pertussis vaccines. it was previously shown that b. parapertussis is able to avoid bacterial killing by polymorphonuclear leukocytes (pmn) if specific opsonic antibodies are not present at the site of interaction. here, we evaluated the outcome of b. parapertussis innate interaction with human macrophages, a less aggr ... | 2014 | 25267839 |
| fibronectin-degrading activity of trypanosoma cruzi cysteine proteinase plays a role in host cell invasion. | trypanosoma cruzi, the agent of chagas disease, binds to diverse extracellular matrix proteins. such an ability prevails in the parasite forms that circulate in the bloodstream and contributes to host cell invasion. whether this also applies to the insect-stage metacyclic trypomastigotes, the developmental forms that initiate infection in the mammalian host, is not clear. using t. cruzi cl strain metacyclic forms, we investigated whether fibronectin bound to the parasites and affected target cel ... | 2014 | 25267835 |
| enhanced transmission of antibiotic resistance in campylobacter jejuni biofilms by natural transformation. | campylobacter jejuni is a leading food-borne pathogen, and its antibiotic resistance is of serious concern to public health worldwide. c. jejuni is naturally competent for dna transformation and freely takes up foreign dna harboring genetic information responsible for antibiotic resistance. in this study, we demonstrate that c. jejuni transfers antibiotic resistance genes more frequently in biofilms than in planktonic cells by natural transformation. | 2014 | 25267685 |
| small-molecule inhibitors of the pseudaminic acid biosynthetic pathway: targeting motility as a key bacterial virulence factor. | helicobacter pylori is motile by means of polar flagella, and this motility has been shown to play a critical role in pathogenicity. the major structural flagellin proteins have been shown to be glycosylated with the nonulosonate sugar, pseudaminic acid (pse). this glycan is unique to microorganisms, and the process of flagellin glycosylation is required for h. pylori flagellar assembly and consequent motility. as such, the pse biosynthetic pathway offers considerable potential as an antivirulen ... | 2014 | 25267679 |
| global transcriptional start site mapping using differential rna sequencing reveals novel antisense rnas in escherichia coli. | while the model organism escherichia coli has been the subject of intense study for decades, the full complement of its rnas is only now being examined. here we describe a survey of the e. coli transcriptome carried out using a differential rna sequencing (drna-seq) approach, which can distinguish between primary and processed transcripts, and an automated prediction algorithm for transcriptional start sites (tss). with the criterion of expression under at least one of three growth conditions ex ... | 2014 | 25266388 |
| global transcriptional start site mapping using differential rna sequencing reveals novel antisense rnas in escherichia coli. | while the model organism escherichia coli has been the subject of intense study for decades, the full complement of its rnas is only now being examined. here we describe a survey of the e. coli transcriptome carried out using a differential rna sequencing (drna-seq) approach, which can distinguish between primary and processed transcripts, and an automated prediction algorithm for transcriptional start sites (tss). with the criterion of expression under at least one of three growth conditions ex ... | 2014 | 25266388 |
| case report: brucella induced guillain-barré syndrome. | neurobrucellosis is relatively uncommon. in a prospective study of 530 patients with brucellosis, neurologic involvement was reported in only 1.7% of the patients. unlike campylobacter jejuni, the commonest infection implicated in guillain-barré syndrome, there are very few reports in the literature of guillain-barré syndrome in association with brucellosis. out of 1,028 cases of brucellosis, polyneuritis was reported in only 2 out of 58 patients with neurological involvement. | 2014 | 25266352 |
| letter from the editor. | 2014 | 25264687 | |
| incidence of and factors associated with false positives in laboratory diagnosis of norovirus infection by amplification of the rna-dependent rna polymerase gene. | conventional reverse transcription-polymerase chain reaction (rt-pcr) amplification of the rna-dependent rna polymerase (rdrp) gene remains a used method for the rapid detection of norovirus (nv) in clinical laboratories. the incidence of and factors associated with false positives in this assay have not been previously evaluated. | 2014 | 25264621 |
| lactobacillus gasseri sbt2055 reduces infection by and colonization of campylobacter jejuni. | campylobacter is a normal inhabitant of the chicken gut. pathogenic infection with this organism in humans is accompanied by severe inflammation of the intestinal mucosal surface. the aim of this study was to evaluate the ability of lactobacillus gasseri sbt2055 (lg2055) to inhibit the adhesion and invasion of campylobacter jejuni in vitro and to suppress c. jejuni colonization of chicks in vivo. pretreatment with lg2055 significantly reduced adhesion to and invasion of a human epithelial cell l ... | 2014 | 25264604 |
| epithelial il-22ra1-mediated fucosylation promotes intestinal colonization resistance to an opportunistic pathogen. | our intestinal microbiota harbors a diverse microbial community, often containing opportunistic bacteria with virulence potential. however, mutualistic host-microbial interactions prevent disease by opportunistic pathogens through poorly understood mechanisms. we show that the epithelial interleukin-22 receptor il-22ra1 protects against lethal citrobacter rodentium infection and chemical-induced colitis by promoting colonization resistance against an intestinal opportunistic bacterium, enterococ ... | 2014 | 25263220 |
| glycoarrays with engineered phages displaying structurally diverse oligosaccharides enable high-throughput detection of glycan-protein interactions. | glycan microarrays have become a powerful platform to investigate the interactions of carbohydrates with a variety of biomolecules. however, the number and diversity of glycans available for use in such arrays represent a key bottleneck in glycan array fabrication. to address this challenge, we describe a novel glycan array platform based on surface patterning of engineered glycophages that display unique carbohydrate epitopes. specifically, we show that glycophages are compatible with surface i ... | 2014 | 25263089 |
| glycoarrays with engineered phages displaying structurally diverse oligosaccharides enable high-throughput detection of glycan-protein interactions. | glycan microarrays have become a powerful platform to investigate the interactions of carbohydrates with a variety of biomolecules. however, the number and diversity of glycans available for use in such arrays represent a key bottleneck in glycan array fabrication. to address this challenge, we describe a novel glycan array platform based on surface patterning of engineered glycophages that display unique carbohydrate epitopes. specifically, we show that glycophages are compatible with surface i ... | 2014 | 25263089 |
| antimicrobial susceptibility profiles and molecular typing of campylobacter jejuni and campylobacter coli isolates from ducks in south korea. | campylobacter is a food-borne zoonotic pathogen that causes human gastroenteritis worldwide. campylobacter bacteria are commensal in the intestines of many food production animals, including ducks and chickens. the objective of the study was to determine the prevalence of campylobacter species in domestic ducks, and the agar dilution method was used to determine resistance of the isolates to eight antibiotics. in addition, multilocus sequence typing (mlst) was performed to determine the sequence ... | 2014 | 25261524 |
| campylobacter jejuni motility is required for infection of the flagellotropic bacteriophage f341. | previous studies have identified a specific modification of the capsular polysaccharide as receptor for phages that infect campylobacter jejuni. using acapsular kpsm mutants of c. jejuni strains nctc11168 and nctc12658, we found that bacteriophage f341 infects c. jejuni independently of the capsule. in contrast, phage f341 does not infect c. jejuni nctc11168 mutants that either lack the flagellar filaments (δflaab) or that have paralyzed, i.e., nonrotating, flagella (δmota and δflgp). complement ... | 2014 | 25261508 |
| lymphoma caused by intestinal microbiota. | the intestinal microbiota and gut immune system must constantly communicate to maintain a balance between tolerance and activation: on the one hand, our immune system should protect us from pathogenic microbes and on the other hand, most of the millions of microbes in and on our body are innocuous symbionts and some can even be beneficial. since there is such a close interaction between the immune system and the intestinal microbiota, it is not surprising that some lymphomas such as mucosal-asso ... | 2014 | 25257357 |
| microbial imbalance and intestinal pathologies: connections and contributions. | microbiome analysis has identified a state of microbial imbalance (dysbiosis) in patients with chronic intestinal inflammation and colorectal cancer. the bacterial phylum proteobacteria is often overrepresented in these individuals, with escherichia coli being the most prevalent species. it is clear that a complex interplay between the host, bacteria and bacterial genes is implicated in the development of these intestinal diseases. understanding the basic elements of these interactions could hav ... | 2014 | 25256712 |
| structural and biochemical characterization of chlamydia trachomatis hypothetical protein ct263 supports that menaquinone synthesis occurs through the futalosine pathway. | the obligate intracellular human pathogen chlamydia trachomatis is the etiological agent of blinding trachoma and sexually transmitted disease. genomic sequencing of chlamydia indicated this medically important bacterium was not exclusively dependent on the host cell for energy. in order for the electron transport chain to function, electron shuttling between membrane-embedded complexes requires lipid-soluble quinones (e.g. menaquionone or ubiquinone). the sources or biosynthetic pathways requir ... | 2014 | 25253688 |
| genome-wide metabolic re-annotation of ashbya gossypii: new insights into its metabolism through a comparative analysis with saccharomyces cerevisiae and kluyveromyces lactis. | ashbya gossypii is an industrially relevant microorganism traditionally used for riboflavin production. despite the high gene homology and gene order conservation comparatively with saccharomyces cerevisiae, it presents a lower level of genomic complexity. its type of growth, placing it among filamentous fungi, questions how close it really is from the budding yeast, namely in terms of metabolism, therefore raising the need for an extensive and thorough study of its entire metabolism. this work ... | 2014 | 25253284 |
| uracil-dna glycosylases-structural and functional perspectives on an essential family of dna repair enzymes. | uracil-dna glycosylases (udgs) are evolutionarily conserved dna repair enzymes that initiate the base excision repair pathway and remove uracil from dna. the udg superfamily is classified into six families based on their substrate specificity. this review focuses on the family i enzymes since these are the most extensively studied members of the superfamily. the structural basis for substrate specificity and base recognition as well as for dna binding, nucleotide flipping and catalytic mechanism ... | 2014 | 25252105 |
| the structure and regulation of flagella in bacillus subtilis. | bacterial flagellar motility is among the most extensively studied physiological systems in biology, but most research has been restricted to using the highly similar gram-negative species escherichia coli and salmonella enterica. here, we review the recent advances in the study of flagellar structure and regulation of the distantly related and genetically tractable gram-positive bacterium bacillus subtilis. b. subtilis has a thicker layer of peptidoglycan and lacks the outer membrane of the gra ... | 2014 | 25251856 |
| prevention of bacterial foodborne disease using nanobiotechnology. | foodborne disease is an important source of expense, morbidity, and mortality for society. detection and control constitute significant components of the overall management of foodborne bacterial pathogens, and this review focuses on the use of nanosized biological entities and molecules to achieve these goals. there is an emphasis on the use of organisms called bacteriophages (phages: viruses that infect bacteria), which are increasingly being used in pathogen detection and biocontrol applicati ... | 2014 | 25249756 |
| variation in the oc locus of acinetobacter baumannii genomes predicts extensive structural diversity in the lipooligosaccharide. | lipooligosaccharide (los) is a complex surface structure that is linked to many pathogenic properties of acinetobacter baumannii. in a. baumannii, the genes responsible for the synthesis of the outer core (oc) component of the los are located between ilve and asps. the content of the oc locus is usually variable within a species, and examination of 6 complete and 227 draft a. baumannii genome sequences available in genbank non-redundant and whole genome shotgun databases revealed nine distinct n ... | 2014 | 25247305 |
| hijacking bacterial glycosylation for the production of glycoconjugates, from vaccines to humanised glycoproteins. | glycosylation or the modification of a cellular component with a carbohydrate moiety has been demonstrated in all three domains of life as a basic post-translational process important in a range of biological processes. this review will focus on the latest studies attempting to exploit bacterial n-linked protein glycosylation for glycobiotechnological applications including glycoconjugate vaccine and humanised glycoprotein production. the challenges that remain for these approaches to reach full ... | 2014 | 25244672 |
| hijacking bacterial glycosylation for the production of glycoconjugates, from vaccines to humanised glycoproteins. | glycosylation or the modification of a cellular component with a carbohydrate moiety has been demonstrated in all three domains of life as a basic post-translational process important in a range of biological processes. this review will focus on the latest studies attempting to exploit bacterial n-linked protein glycosylation for glycobiotechnological applications including glycoconjugate vaccine and humanised glycoprotein production. the challenges that remain for these approaches to reach full ... | 2014 | 25244672 |
| sensitive and rapid detection of campylobacter species from stools of children with diarrhea in japan by the loop-mediated isothermal amplification method. | we detected campylobacter spp. in 5% (20/380) of diarrheal stool samples collected at an outpatient clinic in kyoto using a commercial loop-mediated isothermal amplification (lamp) kit with a fluorescent detection reagent after dna extraction. the sensitivity and specificity were 100% in comparison with those of semi-nested pcr for the differentiation of campylobacter jejuni and campylobacter coli. fourteen of the 20 samples were already determined as c. jejuni by the culture method. all 20 samp ... | 2014 | 25241688 |