Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| antifungal activity of hwa-138 and amphotericin b in experimental systemic candidiasis. | hwa-138, a pentoxifylline analog, has been shown to increase yeast urinary clearance and to reduce yeast counts in the kidneys of rats infected with candida albicans. furthermore, hwa-138 has also been shown to prevent amphotericin b-induced acute renal failure in rats. we report here on the effects of hwa-138 alone and in combination with amphotericin b in the treatment of systemic candidiasis in mice. when single doses of hwa-138 were administered intravenously (10, 25, or 50 mg/kg of body wei ... | 1991 | 1759826 |
| purification, characterization, cloning, and amino acid sequence of the bifunctional enzyme 5,10-methylenetetrahydrofolate dehydrogenase/5,10-methenyltetrahydrofolate cyclohydrolase from escherichia coli. | we have purified the enzyme 5,10-methylenetetrahydrofolate dehydrogenase (ec 1.5.1.5) from escherichia coli to homogeneity by a newly devised procedure. the enzyme has been purified at least 2,000-fold in a 31% yield. the specific activity of the enzyme obtained is 7.4 times greater than any previous preparation from this source. the purified enzyme is specific for nadp. the protein also contains 5,10-methenyltetrahydrofolate cyclohydrolase (ec 3.5.4.9) activity. sodium dodecyl sulfate-polyacryl ... | 1991 | 1748668 |
| striking structural and functional similarities suggest that intestinal sucrase-isomaltase, human lysosomal alpha-glucosidase and schwanniomyces occidentalis glucoamylase are derived from a common ancestral gene. | sequence comparison of the primary structure of the yeast schwanniomyces occidentalis glucoamylase (gam) with gams in different microorganisms did not reveal significant similarities. by contrast, striking similarities were, surprisingly, found with 3 mammalian secretory and integral membrane proteins: the 2 subunits of intestinal brush border sucrase-isomaltase and human lysosomal alpha-glucosidase. the similarities among these proteins are found as clusters of up to 8 amino acids and distribut ... | 1991 | 1743281 |
| mouse pulmonary cytochrome p-450 naphthalene hydroxylase: cdna cloning, sequence, and expression in saccharomyces cerevisiae. | we have isolated a cdna clone, nah-2, encoding the cytochrome p-450nah (naphthalene hydroxylase) from a mouse lung lambda zap cdna library using anti-cytochrome p-450nah igg as a probe. this same antibody selectively blocked [nagata, k., martin, b.m., gillette, j.r., & sasame, h.a. (1990) drug metab. dispos. 18, 557-564] the cytochrome p-450 in mouse lung microsomes that catalyzed the conversion of naphthalene to (1r,2s)-naphthalene 1,2-oxide, which has been postulated as a causative agent in th ... | 1991 | 1742282 |
| increased sterigmatocystin-induced mutation frequency in saccharomyces cerevisiae expressing cytochrome p450 cyp2b1. | 1992 | 1739423 | |
| engineering mammalian aspartyl-trna synthetase to probe structural features mediating its association with the multisynthetase complex. | aspartyl-trna synthetase from higher eukaryotes is a component of a multienzyme complex comprising nine aminoacyl-trna synthetases. the cdna encoding cytoplasmic rat liver aspartyl-trna synthetase was previously cloned and sequenced. this work reports the identification of structural features responsible for its association within the multisynthetase complex. mutant and chimeric proteins have been expressed in mammalian cells and their structural behavior analyzed. a wild-type rat liver aspartyl ... | 1992 | 1735430 |
| primary structure of the thermoplasma proteasome and its implications for the structure, function, and evolution of the multicatalytic proteinase. | the proteasome or multicatalytic proteinase is a high molecular mass multisubunit complex ubiquitous in eukaryotes but also found in the archaebacterial proteasome is made of two different subunits only, and yet the complexes are almost identical in size and shape. cloning and sequencing the gene encoding the small (beta) subunit of the t. acidophilum complex completes the primary structure of the archaebacterial proteasome. the similarity of the derived amino acid sequences of 233 (alpha) and 2 ... | 1992 | 1734972 |
| nucleotide sequence of murine pcna: interspecies comparison of the cdna and the 5' flanking region of the gene. | proliferating cell nuclear antigen (pcna) rna levels are regulated by transcription as well as changes in stability, in growing cells. we have cloned the murine pcna cdna and a fragment of the murine pcna gene flanking the transcription initiation site. comparison of the murine deduced amino acid sequence with the pcna sequence from rat, human, drosophila, saccharomyces cerevisiae, and higher plants, reveals extensive homology between species. the homology is likely to be related to the fundamen ... | 1991 | 1726365 |
| a triple staining procedure to evaluate phagocytic role of differentiated astrocytes. | to determine whether phagocytic activity is affected by a viral infection known to induce astrocyte differentiation, a triple procedure (pap labeling for gfap, pas reaction for added baker's yeast cells and hematoxylin for nuclear staining of the whole monolayer) was applied to junin virus-inoculated cultures, as well as matched controls. the three-step staining simplified yeast cell count for subsequent statistical analysis, which discerned preferential uptake by differentiated rather than imma ... | 1991 | 1724055 |
| alpha 2-macroglobulin is not an acute-phase protein in the rat testis. | earlier studies from this laboratory have shown that sertoli cells actively synthesize and secrete a nonspecific protease inhibitor in vitro; n-terminal sequence analysis, subunit structural analysis, and other biological studies revealed that this protein is the homolog of serum alpha 2-macroglobulin. we have now quantified the relative distribution of alpha 2-macroglobulin in the reproductive compartments and their comparison with nonreproductive organs. in serum and all nonreproductive tissue ... | 1991 | 1709856 |
| properties of a rat monoclonal antibody reactive with both the mannan of candida species and the o-antigen 6,7 polysaccharide of serogroup c1 salmonellae. | monoclonal antibody masc1-mr9 of isotype immunoglobulin m was generated in lou/c rats by immunization with heat-killed salmonella thompson (serogroup c1, o-antigen 6,7 lipopolysaccharide) bacteria which had been further enriched for o-antigen by coating with homologous lipopolysaccharide. eight monoclonal antibodies were selected by screening against the lipopolysaccharide of s. thompson. in a subsequent test against candida mannan, only one antibody, masc1-mr9, was reactive. immunofluorescence ... | 1991 | 1702760 |
| antigenic conservation of primary structural regions of s-adenosylmethionine synthetase. | although the physical and kinetic properties of s-adenosylmethionine (adomet) synthetases from different sources are quite different, it appears that these enzymes have structurally or antigenically conserved regions as demonstrated by studies with adomet synthetase specific antibodies. polyclonal anti-human lymphocyte adomet synthetase crossreacted with enzyme from rat liver (beta isozyme), escherichia coli and yeast. in addition, polyclonal anti-e. coli enzyme and antibodies to synthetic pepti ... | 1990 | 1698095 |
| copolymers of glutamic acid and tyrosine are potent inhibitors of oocyte casein kinase ii. | polypeptides rich in glutamic acid are strong inhibitors purified from isolated nuclei of xenopus laevis oocytes of casein kinase ii. the presence of tyrosine in these peptides greatly enhances their inhibitory capacity. using casein as a substrate, copolyglu:tyr (4:1) has an i50 value of 20 nm, 250 fold lower than that of polyglutamic acid which is 5 microm. a similar large difference is observed when a synthetic peptide is used as substrate. the inhibition of copolyglu:tyr is competitive with ... | 1990 | 1694780 |
| expression of mammalian tyrosine aminotransferase in saccharomyces cerevisiae and escherichia coli. purification to homogeneity and characterization of the enzyme overproduced in the bacteria. | rat liver tyrosine aminotransferase has been expressed in saccharomyces cerevisiae and escherichia coli. in yeast, the extent of production is 20-fold higher than that in rat liver after induction by dexamethasone, and reaches 250-fold higher in an e. coli strain carrying the t7 rna polymerase transcription system. about 250 mg pure and homogeneous enzyme was obtained from 50 g transformed e. coli cells. determination of mr and pi, as well as analysis of n- and c-terminal amino acids, suggest th ... | 1991 | 1682148 |
| inhibitors of metabolic reactions. scope and limitation of acyl-coa-analogue coa-thioethers. | substrate and intermediate analogue inhibitors of enzymes were prepared in which the thioester oxygen of acyl-coa substrates is replaced by hydrogen with formation of coa-thioethers. experiments performed with atp citrate lyase and s-(3,4-dicarboxy-3-hydroxybutyl)-coa are consistent with citryl-coa but not with citryl-enzyme being the direct precursor of the products acetyl-coa and oxaloacetate. consistent with these results, a previously described isotopic exchange between acetyl-coa and [3h]co ... | 1991 | 1675605 |
| free radical scavenging action of bio-catalyzer alpha.rho no.11 (bio-normalyzer) and its by-product. | bio-catalyzer alpha.rho no.11 (bio-normalyzer) and its by-product are natural health products made by yeast fermentation of glucose, carica papaya linn., pennisetum pupureum schum., and sechium edule swartz. their effects on free radicals were examined by electron spin resonance spectrometry using spin trapping agent 5,5-dimethyl-1-pyrroline-1-oxide (dmpo). it was observed that both bio-catalyzer and its by-product scavenged 95% of dmpo-oh spin adducts (89 x 10(15) spins/ml) generated by feso4-h ... | 1991 | 1665836 |
| preparative isolation of polyphosphoinositides and other anionic phospholipids from natural sources using chromatography on adsorbents containing primary amino groups. | a new method for the preparative isolation of anionic phospholipids with the use of chromatography on adsorbents containing primary amino groups has been developed. the method combines elements of bioaffinity and ion-exchange chromatography. synthesis of adsorbents based on spheron and silica supports with immobilized neomycin, l-lysine, or aminoalkyl groups was carried out. optimal conditions for the separation of phospholipid mixtures on aminosorbents were determined. separation of rat brain b ... | 1991 | 1663766 |
| interferon-inducible mouse mx1 protein that confers resistance to influenza virus is gtpase. | the murine mx1 protein is an interferon-inducible nuclear protein and confers resistance to influenza virus infection even though the resistance mechanism is yet unclear. the mx1 protein contains a tripartite gtp-binding domain consisting of gxxxxgks, dxxg, and t/nkxd motifs. in the gtpase gene superfamily such as p21ras protein, signal-transducing g protein, and translation elongation factor, the gtpase activity plays a key role in each protein function. here we show that gtpase activity is ind ... | 1991 | 1657964 |
| liposomes and pulmonary alveolar macrophages: functional and morphologic interactions. | in vitro toxicity of liposomes and their functional and morphologic interactions with rat pulmonary alveolar macrophage (ams) were investigated using viability (trypan blue exclusion), phagocytic and killing activity (uptake and digestion of live s. cerevisiae), surface adherence, respiratory burst (nitro-blue tetrazolium reduction), and morphometry (computerized image analysis) as indicators. liposome stability in physiologic solutions and uptake of liposome-encapsulated carboxyfluorescein (cf) ... | 1991 | 1657589 |
| specificity of leaf mitochondrial and chloroplast processing systems for nuclear-encoded precursor proteins. | the specificity of the mitochondrial and chloroplast processing enzymes for the nuclear-encoded precursor proteins was investigated. mitochondrial precursor proteins of the nicotiana plumbaginifolia and the neurospora crassa beta subunits of f1-atpase and the neurospora rieske fes precursor protein were processed to the correct mature size by matrix extracts isolated from spinach leaves, yeast, rat liver and beef heart. the mitochondrial extracts failed to process chloroplast precursor proteins ... | 1991 | 1654154 |
| an inducible functional peripheral benzodiazepine receptor in mitochondria of steroidogenic granulosa cells. | granulosa cell lines, transformed by sv40 t-antigen and ha-ras oncogene, have recently been established that can produce progesterone at levels comparable to those of highly differentiated cultures of primary granulosa cells (1-4). here, the hypothesis that these cells contain a mitochondrial benzodiazepine receptor, and that stimulation of the receptor can trigger progesterone production in these cells, was tested. the agonist of the peripheral benzodiazepine receptor, ro5-4864, produced a 3- t ... | 1991 | 1647307 |
| the saccharomyces cerevisiae genes (cmp1 and cmp2) encoding calmodulin-binding proteins homologous to the catalytic subunit of mammalian protein phosphatase 2b. | saccharomyces cerevisiae genomic clones that encode calmodulin-binding proteins were isolated by screening a lambda gt11 expression library using 125i-labeled calmodulin as probe. among the cloned yeast genes, we found two closely related genes (cmp1 and cmp2) that encode proteins homologous to the catalytic subunit of phosphoprotein phosphatase. the presumed cmp1 protein (62,999 da) and cmp2 protein (68,496 da) contain a 23 amino acid sequence very similar to those identified as calmodulin-bind ... | 1991 | 1646387 |
| rat procathepsin b. proteolytic processing to the mature form in vitro. | expression of rat procathepsin b in yeast led to the secretion of both the latent and mature forms of the enzyme. culture in the presence of a cysteine proteinase inhibitor prevented this processing. we have expressed and purified a mutant form of rat procathepsin b whose active-site cysteine residue has been changed to a serine, and which also lacks the glycosylation site in the mature region of the protein. this non-active mutant protein was secreted essentially in an unprocessed form. the pur ... | 1992 | 1639824 |
| molecular mimicry: uveitis induced in macaca fascicularis by microbial protein having sequence homology with retinal s-antigen. | s-antigen (s-ag), a well characterized 45-kda protein in the photoreceptor cells, induces predominantly t-cell-mediated autoimmune uveitis when injected into experimental animals. recently, we have shown that native histone h3 protein derived from yeast (saccharomyces cerevisiae), or a synthetic peptide that is homologous with s-ag peptide m in having six consecutive amino acids, induces experimental autoimmune uveitis (eau) similar to that induced by native s-ag in the lewis rat. in this study, ... | 1992 | 1635290 |
| serine and threonine catabolism in saccharomyces cerevisiae: the cha1 polypeptide is homologous with other serine and threonine dehydratases. | the catabolic l-serine (l-threonine) dehydratase of saccharomyces cerevisiae allows the yeast to grow on media with l-serine or l-threonine as sole nitrogen source. previously we have cloned the cha1 gene by complementation of a mutant, cha1, lacking the dehydratase activity. here we present the dna sequence of a 1,766-bp fragment of the cha1 region encompassing an open reading frame of 1080 bp. comparison of the predicted amino acid sequence of the cha1 polypeptide with that of other serine/thr ... | 1992 | 1628804 |
| phospholipid dependence of rat liver microsomal acyl:coa synthetase and acyl-coa:1-acyl-sn-glycero-3-phosphocholine o-acyltransferase. | investigations were performed on the influence of the phospholipid composition and physicochemical properties of the rat liver microsomal membranes on acyl-coa synthetase and acyl-coa:1-acyl-sn-glycero-3-phosphocholine o-acyltransferase activities. the phospholipid composition of the membranes was modified by incubation with different phospholipids in the presence of lipid transfer proteins or by partial delipidation with exogenous phospholipase c and subsequent enrichment with phospholipids. th ... | 1992 | 1625322 |
| rat cystathionine beta-synthase. gene organization and alternative splicing. | we elucidated the structure and alternative splicing patterns of the rat cystathionine beta-synthase gene. the gene is 20-25 kilobase pairs long, and its coding region is divided into 17 exons. these are alternatively spliced, forming four distinct mrnas (types i through iv). the predicted open reading frames encode proteins of 61.5, 39, 60, and 52.5 kda, respectively. exons 13 and 16 are used alternatively and mutually exclusively. exon 13 includes a stop codon and encodes the unique carboxyl-t ... | 1992 | 1597473 |
| [a comparative study of the effect of pyrazole on the activity of enzymes in the alcohol/polyol dehydrogenase family]. | a comparative study of the effect of pyrazol, an inhibitor of the coenzyme-binding site of alcohol dehydrogenases, on the activity of enzymes of the alcohol/polyol dehydrogenase group has been carried out. commercial preparations of alcohol dehydrogenases from the cytoplasm of horse liver cells and yeast cells, as well as the enzyme from the cytoplasm of trichosporon pullulans cells was completely inhibited by 1 mm pyrazol, while alcohol dehydrogenases from candida utilis and saccharomyces carls ... | 1992 | 1594544 |
| alanine and hyperosmolarity are responsible for the stimulation of cardiomyocyte glucose transport by samples containing a glucose tolerance factor. | low-molecular-weight, cationic samples, that were previously reported to contain a glucose tolerance factor, were obtained by partial purification from yeast extract. these samples increased the rate of glucose transport in isolated cardiomyocytes 2.0- to 2.5-fold. a further purification by gel filtration led to the separation of two active components that were identified as (i) l-alanine and (ii) an elevated osmolarity. moreover, the effect of partially purified fractions (before gel filtration ... | 1992 | 1593925 |
| elevated expression of the ribosomal protein s2 gene in human tumors. | differential screening of a cdna library was used to isolate genes differentially expressed in a nontumorigenic clone and a ras-transformed variant of the human teratocarcinoma cell line pa-1. the rna transcript for one of the cdna clones that we identified was expressed at a 25-fold higher level in the ras-transformed pa-1 cells than in the nontumorigenic pa-1 cells. dna sequence analysis of this clone showed that it had 86% nucleic acid homology to the mouse llrep3 gene and only differed at a ... | 1992 | 1586449 |
| metabolism of trans,trans-muconaldehyde by aldehyde and alcohol dehydrogenases: identification of a novel metabolite. | the metabolism of trans,trans-muconaldehyde (ma), a highly reactive alpha,beta-unsaturated dialdehyde, was examined in vitro using purified yeast alcohol and aldehyde dehydrogenases (adh and aldh, respectively). in the presence of nad(+)-fortified aldh, the mono-oxidation product (acid/aldehyde) was the primary metabolite formed with trace amounts of the dioxidation product (trans,trans-muconic acid). in nadh-fortified reactions with adh, both the mono- and direduction products (hydroxy/aldehyde ... | 1992 | 1585367 |
| specific in vitro o-glycosylation of human granulocyte-macrophage colony-stimulating-factor-derived peptides by o-glycosyltransferases of yeast and rat liver cells. | human granulocyte-macrophage colony-stimulating factor (hgm-csf) is o-glycosylated at residues ser9 and thr10 during secretion by yeast and cos-1 cells [ernst, j.f., mermod, j.-j. and richman, l.i. (1992) eur. j. biochem. 203, 663-667]. two types of octapeptides encompassing residues 4-11 (peptide 4-11) and variants thereof, or residues 8-15 (peptide 8-15) of hgm-csf were tested as substrates for in vitro o-glycosylation using dolichyl-phosphate- d-mannose: protein o-d-mannosyltransferase (man-t ... | 1992 | 1576999 |
| uroporphyrinogen decarboxylase in saccharomyces cerevisiae. hem12 gene sequence and evidence for two conserved glycines essential for enzymatic activity. | the hem12 gene from saccharomyces cerevisiae encodes uroporphyrinogen decarboxylase which catalyzes the sequential decarboxylation of the four acetyl side chains of uroporphyrinogen to yield coproporphyrinogen, an intermediate in protoheme biosynthesis. the gene was isolated by functional complementation of a hem12 mutant. sequencing revealed that the hem12 gene encodes a protein of 362 amino acids with a calculated molecular mass of 41,348 da. the amino acid sequence shares 50% identity with hu ... | 1992 | 1576986 |
| mechanism of assembly of the rna polymerase ii preinitiation complex. evidence for a functional interaction between the carboxyl-terminal domain of the largest subunit of rna polymerase ii and a high molecular mass form of the tata factor. | genetic evidence argues that the highly conserved carboxyl-terminal domain (ctd) of the largest subunit of rna polymerase ii functions directly in the regulation of transcription of many eukaryotic genes. the observation that partial deletion of the ctd of yeast rna polymerase ii reduces the ability of the enzyme to respond to signals from a variety of upstream activating sequences led to the proposal that the ctd plays a role in the dialogue between regulatory factors that bind upstream activat ... | 1992 | 1569096 |
| opposite responses of nuclear spermidine n8-acetyltransferase and histone acetyltransferase activities to regenerative stimuli in rat liver. | experiments performed in different models of hepatic regeneration at the time of maximal dna synthesis, determined by thymidine kinase activity assay, demonstrated that spermidine n8-acetyltransferase activity increased 48 hr after ccl4 administration (2-fold), 72 hr after ccl4 plus phenobarbital (3-fold) and 24 hr after partial hepatectomy (4.5-fold). on the contrary, at these times histone acetyltransferase activity diminished (approximately twofold) and was unchanged compared with control val ... | 1992 | 1568734 |
| conserved residues flanking the thiol/disulfide centers of protein disulfide isomerase are not essential for catalysis of thiol/disulfide exchange. | protein disulfide isomerase (pdi) catalyzes the oxidative folding of proteins containing disulfide bonds by increasing the rate of disulfide bond rearrangements which normally occur during the folding process. the amino acid sequences of the n- and c-terminal redox active sites (pwcghck) in pdi are completely conserved from yeast to man and display considerable identity with the redox-active center of thioredoxin (ewcgpck). available data indicate that the two thiol/disulfide centers of pdi can ... | 1992 | 1567868 |
| inhibition kinetics of hepatic microsomal long chain fatty acid-coa ligase by 2-arylpropionic acid non-steroidal anti-inflammatory drugs. | microsomal long chain fatty acid coa ligase (ec 6.2.1.3) has been implicated in the formation of coa thioesters of xenobiotics containing a carboxylic acid moiety. in this study we have demonstrated that the microsomal enzyme from rat liver exhibits biphasic kinetics for the formation of palmitoyl-coa, i.e. there are high affinity low capacity kmhigh, 1.6 microm, vmaxhigh, 12.9 nmol/mg/min) and low affinity high capacity (kmlow, 506 microm, vmaxlow, 58.3 nmol/mg/min) components. inhibition of th ... | 1992 | 1567471 |
| amino-terminal octapeptides function as recognition signals for the mitochondrial intermediate peptidase. | we have shown previously that cleavage of a number of precursors by the mitochondrial processing peptidase (mpp) requires an intermediate octapeptide (fxxsxxxx) between the mpp cleavage site and the mature protein amino terminus. we show now that these octapeptides, present at the amino termini of the intermediates, direct recognition of these substrates by the mitochondrial intermediate peptidase (mip), leading to formation of mature proteins. synthetic peptides, corresponding to the intermedia ... | 1992 | 1560019 |
| a ribosomal protein is specifically recognized by saporin, a plant toxin which inhibits protein synthesis. | many plants express enzymes which specifically remove an adenine residue from the skeleton of the 28 s rna in the major subunit of the eukaryotic ribosome (ribosome inactivating proteins, rips). the site of action of rips (a4324 in the rrna from rat liver) is in a loop structure whose nucleotide sequence all around the target adenine is also conserved in those species which are completely or partially insensitive to rips. in this paper we identify a covalent complex between saporin (the rip extr ... | 1992 | 1544437 |
| identification of ras and ras-related low-molecular-mass gtp-binding proteins associated with rat lung lamellar bodies. | recent evidence from genetic experiments in yeast and from studies using guanosine triphosphate (gtp) analogues in mammalian cells suggests a key role for low-molecular-mass gtp-binding proteins (lmm-gbps) (mr 19 to 28 kd) in processes of intracellular vesicular sorting and secretion. assembly and exocytosis of the lamellar body (lb), the secretory organelle of the pulmonary alveolar type 2 pneumocyte, may be regulated by lmm-gbps. we used [alpha-32p]gtp binding to western blotted proteins, ultr ... | 1992 | 1540390 |
| 17 beta-estradiol hydroxylation catalyzed by human cytochrome p450 1a1: a comparison of the activities induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin in mcf-7 cells with those from heterologous expression of the cdna. | exposure of mcf-7 breast cancer cells to 2,3,7,8-tetrachlorodibenzo-p-dioxin (tcdd) causes an elevated cytochrome p450 content and a marked increase in the microsomal hydroxylation of 17 beta-estradiol (e2) at the c-2, c-4, c-15 alpha, and c-6 alpha positions. in this study we investigated the involvement of cytochromes p450 of the 1a gene subfamily in this metabolism of e2. hydroxylation at each of these four positions of e2 was inhibited by p450 1a-subfamily inhibitors, alpha-naphthoflavone, b ... | 1992 | 1536570 |
| functional consequences of mutation of highly conserved serine residues, found at equivalent positions in the n- and c-terminal domains of mammalian hexokinases. | despite the extensive sequence similarity between the n- and c-terminal halves of the 100-kda molecular weight mammalian hexokinases (atp:d-hexose 6-phosphotransferase, ec 2.7.1.1), reflecting their evolutionary origin by duplication and fusion of a gene coding for a smaller ancestral hexokinase, there is evidence for a functional division, with the c-terminal domain retaining a catalytic role while the n-terminal domain serves a regulatory function [binding of the product inhibitor, glucose 6-p ... | 1992 | 1524437 |
| affinity labeling of vertebrate oxidosqualene cyclases with a tritiated suicide substrate. | pig and rat liver oxidosqualene cyclase (osc) enzymes were purified to homogeneity and showed single bands on sds-polyacrylamide gel electrophoresis with molecular masses of 75 kda (pig) and 78 kda (rat). pig liver osc was purified for the first time (441-fold with a yield of 39%). chemical affinity labeling of pure or crude preparations of the liver cyclases using the mechanism-based irreversible inhibitor of osc, [3h]29-methylidene-2,3-oxidosqualene ([3h]29-mos), showed a single radioactive ba ... | 1992 | 1520315 |
| [the yeast test: an alternative method for determination of acute toxicity of drugs and environmental chemicals]. | starting point for this study was the urgent need for replacement of the contemporary mode of acute toxicity testing of chemicals in vertebrates (ld50-test) by an experimental model with equal power that can be performed on non-pain sensitive matter. for this purpose, a testing procedure ought to be developed using a non-pathogenic microorganism as testing object that is available at any time, easy to cultivate, and in its indicative power equivalent to the ld50 test in mice, rats, and other lab ... | 1992 | 1518900 |
| oscillations in glycolysis: multifactorial quantitative analysis in muscle extract. | a multifactorial quantitative analysis of oscillations in glycolysis was conducted in the postmicrosomal supernatant of rat muscle homogenates incubated in the presence of yeast hexokinase. oscillations in adenine nucleotides, d-fructose 1,6-bisphosphate, triose phosphates, l-glycerol 3-phosphate, 3hoh generation from d-[5-3h]glucose, nadh and l-lactate production were documented. the occurrence of such oscillations were found to depend mainly on the balance between the consumption of atp associ ... | 1992 | 1518503 |
| antibodies to rat procathepsin b recognize the active mature enzyme. | use of mature cathepsin b for immunization invariably yields antisera that react with the denatured protein but not with the native enzyme. this is thought to be due to spontaneous denaturation of the immunizing antigen on introduction into the animal. recombinant rat procathepsin b has been expressed in yeast as a secreted product. a procathepsin b mutant (cys29ser), where autoprocessing is prevented, has been purified and used to raise a rabbit polyclonal antiserum. both immunodiffusion analys ... | 1992 | 1515070 |
| investigation of structure function relationships in cathepsin b. | previous suggestions from sequence alignment studies and examination of the recently determined x-ray crystal structures of cathepsin b point to roles for several specific residues in substrate binding and catalysis. the role of these groups is being examined by studying cathepsin b mutants produced using a yeast expression system. the substitutions gly198asp, arg202ala, his111gln and glu245gln provide a mechanistic basis for the exopeptidase activity of cathepsin b and the ability of this cyste ... | 1992 | 1515068 |
| [adaptation of rats to unusual protein products during transition to definitive nutrition]. | the authors studied the adaptation of initial and final links of the proteolytic chain, and intermediate nitrogen metabolism, to unusual products--protein concentrate from yeast-saccharomyces and anchovy. a number of enzymes in the organs-producers were found to be activated due to intensification of a limited proteolytic reaction. it was established that the changes in the functioning of the proteolytic stage of the digestive-transport conveyor led to shifts in the amino-acid pool in the blood ... | 1992 | 1514254 |
| distinct and specific gap activities in rat pancreas act on the yeast gtp-binding proteins ypt1 and sec4. | previous studies have demonstrated that sec4, a 23.5 kda guanine nucleotide-binding protein of the ras superfamily is required for exocytosis in the budding yeast saccharomyces cerevisiae. ypt1, another ras-like 23 kda guanine nucleotide-binding protein in yeast has been found to be involved in er-golgi transport. a mammalian homologue of ypt1 called rab1 has also been identified. recent studies using purified sec4 protein have identified a component of yeast lystate that specifically stimulates ... | 1992 | 1511744 |
| 2,3-epoxy-10-aza-10,11-dihydrosqualene, a high-energy intermediate analogue inhibitor of 2,3-oxidosqualene cyclase. | 2,3-epoxy-10-aza-10,11-dihydrosqualene, a high-energy intermediate analogue inhibitor of 2,3-oxidosqualene (so) cyclase was obtained by total synthesis. this involved the preparation of three main building blocks: (1) c17 squalenoid n-methylamine, (2) 3-(diphenylphosphinoyl)propanal, and (3) 5,6-epoxy-6-methylheptan-2-one. the final stages of the reconstruction of the 6e double bond were obtained by a wittig-horner reaction which was modified for poorly reactive systems. this compound was design ... | 1992 | 1501233 |
| determination of free n-acetylamino acids in biological samples and n-terminal acetylamino acids of proteins. | n-acetylamino acids were derivatized with 9-anthryldiazomethane to the corresponding esters. the anthryl esters were separated by high-performance liquid chromatography and detected fluorimetrically (excitation at 365 nm; fluorescence emission measured at 412 nm). n-acetyl derivatives of asn, gln, ser, thr, gly, ala, tyr, pro, met, val, ile and leu as well as n-formyl-met could be separated and identified in the same chromatographic run. the detection limit was from 0.10 pmol for acgln to 5.5 pm ... | 1992 | 1500458 |
| regulatory consequences of organization of citric acid cycle enzymes. | 1992 | 1499336 | |
| evidence for a conserved 95-120 kda subunit associated with and essential for activity of v-atpases. | vacuoles purified from saccharomyces cerevisiae bearing the vph1-1 mutation had no detectable bafilomycin-sensitive atpase activity or atp-dependent proton pumping. furthermore, the vacuolar h(+)-atpase (v-atpase) nucleotide binding subunits were no longer associated with vacuolar membranes yet were present at wild-type levels in yeast whole-cell extracts. the vph1 gene was cloned by screening a lambda gt11 expression library with antibodies directed against a 95 kda vacuolar integral membrane p ... | 1992 | 1491220 |
| effect of nitrite ingestion on the bioavailability of folate in the rat. | the effect of nitrite ingestion on the intestinal deconjugation and absorption of folic acid (pga) and brewers yeast folate was investigated using a rat bioassay and liver folate uptake as the response parameter. male weanling sprague dawley rats were depleted on a low folate ain-76a formulated basal diet for 21 days. during a 14 day repletion period, folic acid (pga) and brewers yeast were added to provide 0.25, 0.5 and 1.0 mg of folate per kg of diet. potassium nitrite was administered as part ... | 1992 | 1473906 |
| cloning, sequencing, and expression of the fadd gene of escherichia coli encoding acyl coenzyme a synthetase. | in the enteric bacterium, escherichia coli, acyl coenzyme a synthetase (fatty acid:coa ligase (amp-forming) ec 6.2.1.3) activates exogenous long-chain fatty acids concomitant with their transport across the inner membrane into metabolically active coa thioesters. these compounds serve as substrates for acyl-coa dehydrogenase in the first step in the process of beta-oxidation. the acyl-coa synthetase structural gene, fadd, has been identified on clone 6d1 of the kohara e. coli gene library and by ... | 1992 | 1460045 |
| characterization and purification of a novel datp-binding protein in eukaryotes. | we characterized and purified an acidic datp-binding protein, which, in its active form, resides in the nuclear fraction of a range of cells from mammals (including pig liver) and baker's yeast (saccharomyces cerevisiae). this protein exhibits a high degree of specificity for the deoxy form of the naturally occurring nucleoside triphosphates and shows a marked preference for the purine deoxynucleoside triphosphates datp and dgtp. the protein cleaves the terminal phosphate of datp and appears to ... | 1992 | 1445246 |
| leu2 gene homolog in kluyveromyces lactis. | a dna fragment that can complement the leu2 mutation of saccharomyces cerevisiae was cloned from the genomic library of kluyveromyces lactis. the nucleotide sequence revealed an open reading frame of 362 codons, 75% homologous to s. cerevisiae leu2 gene. the upstream region contained a ccggaaccgg sequence identical to the site of leucine-specific control of leu2. further upstream, there is a partial open reading frame homologous to rat ribosomal protein l7. | 1992 | 1441757 |
| studies of the catalytic activities and substrate specificities of saccharomyces cerevisiae myristoyl-coenzyme a: protein n-myristoyltransferase deletion mutants and human/yeast nmt chimeras in escherichia coli and s. cerevisiae. | saccharomyces cerevisiae myristoyl-coa:protein n-myristoyltransferase (nmt1p) is an essential, 455-residue, monomeric enzyme. amino- and carboxyl-terminal deletion mutants of nmt1p were genetically engineered to determine the minimal domain necessary to maintain catalytic activity. enzyme activity was assessed by (i) sequentially inducing nmt1p or its mutant derivatives and one of two eukaryotic substrates for the wild type enzyme (s. cerevisiae gpa1p and rat go alpha) in escherichia coli, a bac ... | 1992 | 1429724 |
| identification of breakpoints in t(8;21) acute myelogenous leukemia and isolation of a fusion transcript, aml1/eto, with similarity to drosophila segmentation gene, runt. | we have developed a restriction map of the chromosome 21 breakpoint region involved in t(8;21)(q22;q22.3) acute myelogenous leukemia (aml) and have isolated a genomic junction clone containing chromosome 8 and 21 material. using probes from these regions, rearrangements have been identified in each of nine cases of t(8;21) aml examined. in addition, we have isolated cdna clones from a t(8;21) aml cdna library that contain fused sequences from chromosome 8 and 21. the chromosome 8 component, refe ... | 1992 | 1391946 |
| the vph1 gene encodes a 95-kda integral membrane polypeptide required for in vivo assembly and activity of the yeast vacuolar h(+)-atpase. | yeast vacuolar acidification-defective (vph) mutants were identified using the ph-sensitive fluorescence of 6-carboxyfluorescein diacetate (preston, r. a., murphy, r. f., and jones, e. w. (1989) proc. natl. acad. sci. u.s.a. 86, 7027-7031). vacuoles purified from yeast bearing the vph1-1 mutation had no detectable bafilomycin-sensitive atpase activity or atp-dependent proton pumping. the peripherally bound nucleotide-binding subunits of the vacuolar h(+)-atpase (60 and 69 kda) were no longer ass ... | 1992 | 1385813 |
| effects of supercypermethrin, a synthetic developmental pyrethroid, on four biological test systems. | the genotoxic potential of the insecticide supercypermethrin, a second-generation pyrethroid, was studied on four different test systems. it was non-mutagenic to salmonella typhimurium strains ta1535, ta100, ta1538, ta98 and ta97 in the presence and absence of s9 mixture. it induced gene conversion at the tryptophan locus and induced point mutations at the isoleucine locus in saccharomyces cerevisiae cells. a slight increase in the frequency of aberrant anaphases and telophases in root tips of h ... | 1992 | 1381478 |
| evaluation of genotoxicity of tert.-butylhydroquinone in an hepatocyte-mediated assay with v79 chinese hamster lung cells and in strain d7 of saccharomyces cerevisiae. | tert.-butylhydroquinone (tbhq) has been reported to be genotoxic in some short-term assays but non-genotoxic in others. we have examined cytotoxicity and genotoxicity of tbhq, a principal metabolite of the phenolic antioxidant 2(3)-tert.-butyl-4-hydroxyanisole (bha), in an hepatocyte-mediated assay with v79 chinese hamster lung cells including both sister-chromatid exchange (sce) and thioguanine-resistance (tgr) endpoints. the ability of bha and of tbhq to elicit a genotoxic response in saccharo ... | 1992 | 1377341 |
| induction by fungal elicitor of s-adenosyl-l-methionine synthetase and s-adenosyl-l-homocysteine hydrolase mrnas in cultured cells and leaves of petroselinum crispum. | treatment of cultured parsley (petroselinum crispum) cells with fungal elicitor rapidly activates transcription of many genes encoding specific steps in pathogen defense-related pathways. we report evidence that three cdnas corresponding to such genes represent two key enzymes of the activated methyl cycle. two cdnas are derived from distinct members of the s-adenosyl-l-methionine synthetase gene family, based on extensive similarity of the deduced polypeptides with authentic enzymes from arabid ... | 1992 | 1374911 |
| evaluation of heat sterilization of commercial rat diets for use in fda toxicological studies. | certified commercial rat diets, control and fortified, in the form of pellets and meal, were evaluated in a simulated subchronic rat feeding study. the diets were analyzed before and after autoclaving to determine nutrient integrity and loss, as well as the efficiency of autoclaving for removal of microbiological contaminants. sterilization reduced the level of heat-labile vitamins, but protein level was minimally reduced. sterilization eliminated most of the bacterial contaminants and virtually ... | 1992 | 1371894 |
| a rapid method for measuring the steady state levels of mitochondrial rna in whole mitochondria. | 1992 | 1371187 | |
| the gene encoding the biotin carboxylase subunit of escherichia coli acetyl-coa carboxylase. | we report the molecular cloning and dna sequence of the gene encoding the biotin carboxylase subunit of escherichia coli acetyl-coa carboxylase. the biotin carboxylase gene encodes a protein of 449 residues that is strikingly similar to amino-terminal segments of two biotin-dependent carboxylase proteins, yeast pyruvate carboxylase and the alpha-subunit of rat propionyl-coa carboxylase. the deduced biotin carboxylase sequence contains a consensus atp binding site and a cysteine-containing sequen ... | 1992 | 1370469 |
| comparison of the catalytic and inhibitory properties of pachysolen tannophilus xylose reductase to rat lens aldose reductase. | the catalytic and inhibitory profiles of xylose reductase isolated from the yeast pachysolen tannophilus (ptxr) are compared to those of aldose reductase (ar) obtained from rat lens. while both ptxr and rat lens ar are nadph-specific enzymes and have an affinity for a variety of substrates such as d-xylose, d,l-glyceraldehyde, and 4-nitrobenzaldehyde, the enzymes differ in their substrate affinity profiles. also, ptxr is not inhibited by standard inhibitors of ar thus supporting a hypothesis tha ... | 1992 | 1368496 |
| peroxisomal acetoacetyl-coa thiolase of an n-alkane-utilizing yeast, candida tropicalis. | two genes encoding acetoacetyl-coa thiolase (thiolase i; ec 2.3.1.9), whose localization in peroxisomes was first found with an n-alkane-utilizing yeast, candida tropicalis, were isolated from the lambda embl3 genomic dna library prepared from the yeast genomic dna. nucleotide sequence analysis revealed that both genes contained open reading frames of 1209 bp corresponding to 403 amino acid residues with methionine at the n-terminus, which were named as thiolase ia and thiolase ib. the calculate ... | 1992 | 1362382 |
| isolation and analysis of the fission yeast gene encoding polymerase delta accessory protein pcna. | five monoclonal antibodies raised against rat pcna cross-reacted with a similar protein in the fission yeast schizosaccharomyces pombe. one of these was used to screen an s.pombe cdna expression library. an incomplete cdna was isolated and used to screen a genomic library, identifying a single gene, designated pcn1+ (proliferating cell nuclear antigen). the gene encodes a protein of 260 amino acids, with a deduced sequence 52% identical to human and rat pcnas, which are 98.5% identical to each o ... | 1992 | 1361173 |
| organella-targeted expression of rat liver cytochrome p450c27 in yeast. genetically engineered alteration of mitochondrial p450 into a microsomal form creates a novel functional electron transport chain. | a modified rat cytochrome p450c27, whose mitochondrial targeting signal had been replaced by a possible microsomal targeting signal of bovine cytochrome p450c17, was expressed in yeast. the modified p450c27 hemoprotein was correctly localized on yeast microsomes and exhibited the p450c27-dependent monooxygenase activity by addition of bovine adrenodoxin (adx) and nadph-adrenodoxin reductase (adr). considering the previous observation that p450c27 with its own mitochondrial targeting signal was i ... | 1992 | 1322905 |
| the yeast fbp26 gene codes for a fructose-2,6-bisphosphatase. | sequencing of an open reading frame 450 bp downstream from the yeast vps35 gene revealed a putative peptide of 452 amino acids and 52.7 kda. the predicted amino acid sequence has 45% identity with the 55-kda subunit of the 6-phosphofructo-2-kinase/fructose-2,6- bisphosphatase (ec 2.7.1.105/ec 3.1.3.46) from rat liver and 42% identity with 480 amino acids in the center of the recently reported 93.5-kda subunit of yeast 6-phosphofructo-2-kinase (ec 2.7.1.105). the product of the new yeast gene is ... | 1992 | 1322693 |
| comprehensive sequence analysis of the 182 predicted open reading frames of yeast chromosome iii. | with the completion of the first phase of the european yeast genome sequencing project, the complete dna sequence of chromosome iii of saccharomyces cerevisiae has become available (oliver, s. g., et al., 1992, nature 357, 38-46). we have tested the predictive power of computer sequence analysis of the 176 probable protein products of this chromosome, after exclusion of six problem cases. when the results of database similarity searches are pooled with prior knowledge, a likely function can be a ... | 1992 | 1304897 |
| 4h-thieno[3,4-c]pyrazole derivatives with antiinflammatory, analgesic, antipyretic and platelet antiaggregating activities. | the synthesis of a series of 1-aryl-1,6-dihydro-4h-thieno[3,4-c]pyrazol-4-ones by cyclization of 3-[(2-arylhydrazino)methylene]thiophene-2,4(3h,5h)-diones, prepared by reacting 3-dimethylaminomethylenethiophene-2,4(3h,5h)-dione with arylhydrazines, is described. the 4-fluorophenyl derivative showed remarkable analgesic, antiinflammatory and antipyretic activities in mice or rats, as well as a platelet antiaggregating activity in vitro comparable to that of acetylsalicylic acid. | 1992 | 1294166 |
| insulin activity: stimulatory effects of cinnamon and brewer's yeast as influenced by albumin. | cinnamon and brewer's yeast extracts have been shown to potentiate the action of insulin in isolated adipocytes. in this study, isolated rat epididymal adipocytes were used to evaluate the influence of bovine serum albumin on insulin activity as affected by cinnamon and brewer's yeast extracts. albumin at 0.01-0.1% decreased the insulin stimulatory effects of cinnamon from 11.8- to 5.3-fold and 2% albumin decreased this effect to near control levels. conversely, the insulin-enhancing properties ... | 1992 | 1292975 |
| antibodies directed against a yeast carboxyl-terminal peroxisomal targeting signal specifically recognize peroxisomal proteins from various yeasts. | the carboxyl-terminal tripeptide ala-lys-ile is essential for targeting candida tropicalis trifunctional enzyme (hydratase-dehydrogenase-epimerase) to peroxisomes of both candida albicans and saccharomyces cerevisiae (aitchison,j.d., murray, w.w. and rachubinski, r. a. (1991).j. biol. chem. 266, 23197-23203). we investigated the possibility that this tripeptide may act as a general peroxisomal targeting signal (pts) for other proteins in the yeasts c. tropicalis, c. albicans, yarrowia lipolytica ... | 1992 | 1279909 |
| rat liver cytoplasmic glucose-6-phosphate dehydrogenase. steady-state kinetic properties and circular dichroism. | steady-state kinetic studies including initial velocity, nadph product inhibition, dead-end inhibition, and combined dead-end and product inhibition measurements with purified rat liver glucose-6-phosphate dehydrogenase indicate a sequential and obligatory addition of substrates in the order of nadp+, glucose-6-p for the catalytic pathway at ph 8.0. although instability of 6-phosphoglucono-delta-lactone precluded product inhibition experiments which might directly exclude an enzyme-6-phosphogluc ... | 1976 | 1247537 |
| phenylalanyl-trna synthetases of rat liver: differential effects of thyroid hormone. | thyroxine and analogues inhibit rat liver aminoacyl-trna synthetase activity for phenylalanine and tyrosine. a high yield purification of the major cytoplasmic form of phenylalanyl-trna synthetase (c1) and its characterization is reported. polyribosome-bound and other sedimentable forms are found to be indistinguishable from soluble enzyme by immunoprecipitation. mitochondrial phenylalanyl-trna synthetase (m) and cytoplasmic activity (c2) resistant to anti-c1 antibody have been partially purifie ... | 1976 | 1247520 |
| safety evaluation of yeast grown on hydrocarbons. iv. two-year feeding and multigeneration study in rats with yeast grown on pure n-paraffins. | 1975 | 1205436 | |
| [comparative study of the influence of yeast mannans on the rate of catalase synthesis in the liver of rats]. | the effect of polysaccharides from rh. rubra and rh. mucilaginosa was studied as applied to the rate of catalase synthesis in rat liver. the constants were calculated for the rates of catalase synthesis and destruction. yeast's mannan from rh. mucilaginosa does not practically affect the rate of catalase synthesis. the mannan from rh. rubra produces an almost three-fold inhibition on the catalase synthesis and the degree of the enzyme synthesis recovery lowers by 20% as compared the control valu ... | 1975 | 1202694 |
| lead poisoning in vitamin e-deficient rats. | weanling male rats were fed either a vitamin e-deficient torula yeast diet or the same diet supplemented with 100 ppm vitamin e for a period of 3 months. one group of animals fed each diet received 250 ppm lead in the drinking water, whereas another group of animals fed each diet received no lead in the water. vitamin e deficiency per se had little or no effect on hematocrit value, reticulocyte count, spleen weight, or erythrocyte mechanical fragility in rats not poisoned with lead. on the other ... | 1975 | 1185284 |
| chemistry and pharmacology of naproxen. | the need for a nonsteroidal anti-inflammatory agent effective in rheumatoid arthritis, osteoarthritis, gout, ankylosing spondylitis and related diseases with reduced side effects when compared to existing drugs led us to develop naproxen: d-2-(6'-methoxy-2'-naphthyl)-propionic acid. this new agent is a highly effective anti-inflammatory, analgetic, and antipyretic agent in the rodent administered orally. in a rat paw edema test for anti-inflammatory activity naproxen was 55 times more active tha ... | 1975 | 1173774 |
| candida ingens as a potential fodder protein. | pig wastes were fermented and the supernatant was inoculated at the surface with candida ingens which grew as a pellicle. this was harvested, dried and incorporated with wheat into 2 rations of 20% and 17.9% crude protein as were 2 similar rations using casein and wheat. using rats the rations were compared for 43 d. there was no significant difference in weight gain or feed conversion between the yeast and casein fed rats at 20% crude protein nor was there a difference in weight gain at 17.9% c ... | 1975 | 1167139 |
| structural properties of pyruvate carboxylases from chicken liver and other sources. | varieties of pyruvate carboxylase [pyruvate: co2 ligase (adp-forming), ec 6.4.1.1] obtained from the livers of several species of vertebrates, including humans, all show the same basic structure. they are composed of large polypeptide chains of molecular weights ranging from 1.2 to 1.3 x 10(5) for the different varieties of the enzyme. the native form of the enzyme appears to be a tetramer with a molecular weight of about 5 x 10(5). in the case of pyruvate carboxylase from chicken liver each pol ... | 1975 | 1105579 |
| comparison of membrane organization in mitochondria from yeast and rat liver by nuclear magnetic resonance spectroscopy. | proton magnetic resonance (pmr) and carbon-13 magnetic resonance (cmr) spectra of intact, unsonicated yeast and rat liver motochondria show differences which may be correlated with the composition of the membranes. high resolution pmr and cmr signals in intact yeast mitochondria have been assigned to regions of fluid lipid-lipid interaction on the basis of spectra of extracted lipid and protein, and the temperature dependence of nmr signals from the intact membrane. pmr spectra suggest that abou ... | 1975 | 1104837 |
| site of aminoacylation of trnas from escherichia coli with respect to the 2'- or 3'-hydroxyl group of the terminal adenosine. | a method is presented by which the site of primary attachment of the amino acids with respect to the 2'- or 3'-hydroxyl group of the terminal adenosine of e. coli trnas can be determined. it is found that the aminoacyl-trna synthetases (ec 6.1.1.-) with specificity for arg, asn, ile, leu, met, phe, thr, trp, and val attach the amino acid to the 2'-position; those with specificity for gly, his, lys, and ser attach the amino acid to the 3'-position; and that tyr and cys can be enzymatically attach ... | 1975 | 1103137 |
| a glyoxalase i inhibitor of a new structural type produced by streptomyces. | many streptomyces strains produced an inhibitor of crude glyoxalase prepared from rat liver which did not inhibit glyoxalase i prepared from yeast. another inhibitor, c11h14o6, which inhibited glyoxalases prepared from both rat liver and yeast was obtained from a cultured broth of streptomyces griseosproeus and crystallized. preincubation of this inhibitor with reuduced glutathione increased its inhibitory activity, which suggested its reaction with reduced glutathione. it showed a strong inhibi ... | 1975 | 1102510 |
| mast cell sensitizing antibody (mcsab) response in experimental candidiasis: chromatographic studies. | a single dose of the yeast-like fungus candida albicans (ca), mixed with complete freund's adjuvant (cfa), and inoculated into guinea-pig foot-pads, provoked the productions of homologous mast cell sensitizing antibodies (mcsab) present in the psii-globulin fraction only, and of agglutinins localized in the same fraction. heterologous mcsab and skin-sensitizing antibodies detectable by passive cutaneous anaphylaxis (pca) were constantly absent. these facts are in contradiction to results of prev ... | 1975 | 1098354 |
| synthesis of a mixed disulfide of egg white lysozyme and glutathione - a model substrate for enzymatic reduction of protein mixed disulfides. | 1975 | 1095406 | |
| the isolation of large polysomes in high yield from unfractionated tissue homogenates. | it was found that if large quantities of both exogenous rna and mg-2+ were present during gentle tissue homogenization, the subsequent addition of deoxycholate to the whole homogenate produced a viscous mass from which polysomes could be isolated in large yields. these polysomes were substantially less degraded than those isolated by previous methods. in the case of rat liver, 15 ribosomes per mrna was the species present in highest concentration. the parameters of this method were investigated ... | 1975 | 1095068 |
| active site-specific reagents and transition-state analogs for enolase. | 1975 | 1092960 | |
| sequence homology of nuclear and mitochondrial dnas of different yeasts. | 1. both nuclear and mtdna of four different yeasts show approximately 10% homology as measured by dna-dna filter hybridization. these homologous sequences are mainly attributable to the ribosomal cistrons. 2. melting curve analysis shows that the heterologous mitochondrial dna-dna hybrids contain several times more mismatching than the nuclear dna-dna hybrids. 3. dna-rrna hybridization shows that the sequences of the ribosomal cistrons in both the nuclear and the mitochondrial genome have been c ... | 1975 | 1092349 |
| structure of the fluorescent nucleoside of yeast phenylalanine transfer ribonucleic acid. | 1975 | 1092270 | |
| taxonomical examination and characterization of a methanol-utilizing yeast. | the morphological, cultural, and physiological characteristics are described of a yeast, li70, which uses methanol as its source of energy and carbon; these characteristics have made it possible to identify the strain as candida boidinii ramirez. the identification was confirmed by a dna-dna genetic homology of 99.43% with the type strain of c. boidinii. strain li70 is not pathogenic. | 1976 | 1087866 |
| nutritional evaluation of the protein of dried tomato pomace in the rat. | 1. nutritional evaluation of dried tomato pomace (dtp-20) as a source of protein was carried out using weanling rats. comparisons were made with casein (cs), soya-bean meal (som-45) and the hydrocarbon-grown yeast toprina (bp-t). the growth-promoting effects of the diets were evaluated over a period of 28 d of ad lib. feeding. 2. the unsupplemented dpt-20 had a protein efficiency ratio (per) of 2-18 +/- 0-13 and a net protein utilization (npu) of 0-55. the addition of dl-methionine (5 g/kg) resu ... | 1976 | 1009071 |
| structural studies on rna from bombyx mori l. i. nucleoside composition of enriched trna species from the posterior silkgland purified by coutercurrent distribution. | a large scale fractionation of trna from the posterior silkgland of the silkworm bombyx mori l. by countercurrent distribution is described. one single 1,500 transfer distribution carried out with phosphate buffer-fromamide-isopropanol (pfi) solvent system yields highly enriched isoaccepting species with increasing mobility order: trna1gly, trna1-2ala, trnatyr, trna2gly, trna1ser and trna2ser with 75%, 70%, 90%, 60%, 60%, and 90% purities respectively. nucleosides fingerprint analysis of each is ... | 1976 | 999951 |
| nutritive value of geotricum candidum yeast prepared from decaying fruits in egypt. | 1976 | 987656 | |
| fatty acid composition of adipose tissue and liver fats of the rats fed yeast grown on n-alkanes. | n-alkane assimilating yeast, candida tropicalis yo-148, was grown on an n-alkane-containing medium. a synthetic diet containing 6.8% of dried yeast was fed to rats. the fatty acid composition of adipose tissue and liver fats was determined after a two week feeding period. the percentage of odd-numbered acids increased in the animals fed the yeast diet. furthermore, it was shown that heptadecenoic acid, the major odd-numbered acid in yeast, was accumulated in neutral lipid fraction of adipose tis ... | 1976 | 966078 |
| catalytic activation of transfer ribonucleic acid by a mammalian protein. | a trna activator has been isolated from mammalian organs which increases the capability of trna to accept certain amino acids through the action of mammalian aminoacyl-trna synthetases. this activity may be separated from the aminoacyl-trna synthetases for isoleucine, lysine, serine, and methionine by fractionation of liver or pancreas cytosol with ammonium sulfate or by chromatography over sephadex g-200. the trna activating material is nondialyzable and is destroyed by trypsin or short heating ... | 1976 | 963009 |
| [problem of nitrogen metabolism in monogastric animals]. | a new method has been developed for determining the proportion of endogenic and exogenic faecal n by measuring the degree of specific labelling in the feeding-stuff (administered orally) and in the excreta (faeces and urine). the method has also been developed to find out whether the endogenic excretion of intestinal n is actually independent of the n intake. the present technique had been worked out on the basis of the model that had been originally established by czarnetzki et al. in 1969 with ... | 1976 | 962585 |
| osmotic and peroxidative fragilities of erythrocytes from vitamin e-deficient lead-poisoned rats. | weanling male rats were fed either a vitamin e-deficient torula yeast diet fortified with selenium or the same diet supplemented with 100 ppm vitamin e. one group of rats fed each diet received plain distilled water, whereas another group received 250 ppm lead as lead acetate in the drinking water. after a 3 month feeding period, erythrocyte osmotic and peroxidative fragilities were determined in an osmotic test recorder. dietary vitamin e had little or no effect on the osmotic fragility of red ... | 1977 | 845674 |