Publications

some biochemical changes in mouse after in-vivo irradiation of pancreas with ultrasound.	875 khz continuous wave of ultrasound at 2.5 w/cm2 intensity revealed certain biochemical and enzymological changes in mouse pancreas and liver following the irradiation of pancreas in-vivo for a total of 300 seconds spread over five days. the sacrifice of the animals was carried out on day 0, day 1, day 5 and day 10. blood glucose was reduced significantly with concomitant increase in liver glycogen. glucose-6-phosphatase in liver was decreased significantly while glycogen phosphorylase showed ...	1992	1319155
russell bodies consist of heterogenous glycoproteins in b-cell lymphoma cells.	the phenotypic expression of russell bodies (rb) in the tumor cells of two patients with different types of b-cell lymphoma and of one patient with plasma cell myeloma were examined. in both b-cell lymphomas, the rbs reacted consistently with anti-leu 8 and anti-immunoglobulin m. the rbs in one case also reacted with other monoclonal antibodies, including anti-cd5, cd19, cd22, and cd25. the membranes of most of the tumor cells containing rbs did not stain. in the myeloma, the rbs reacted only wi ...	2005	1317669
the clinical significance of in vitro cytomegalovirus susceptibility to antiviral drugs.		1992	1317603
sleep deprivation-induced hyperthermia following antigen challenge due to opioid but not interleukin-1 involvement.	eight hours total sleep deprivation does not affect colonic temperature. the combination of a subpyrogenic challenge of sheep red blood cells with sleep loss however, can produce a significant rise in colonic temperature that peaks during the third hour of the sleep deprivation vigil. the regulation of this increase in colonic temperature appears to be opioid in nature and not because of the release of the cytokine interleukin-1. it would appear that the combination of sleep loss and low dose an ...	1992	1317589
regulation of pregnenolone synthesis in c6-2b glioma cells by 4'-chlorodiazepam.	an experimental model to study synthesis of cholesterol and pregnenolone from the precursor mevalonolactone (mva) was developed in c6-2b glioma cells. the steroidogenic capability of this cell line and the regulation of pregnenolone production by 4'-chlorodiazepam (4'cd), a specific ligand for the mitochondrial diazepam binding inhibitor (dbi) receptor (mdr), were investigated. cells maintained in serum-free media were incubated with lovastatin (20 microm) and two inhibitors of pregnenolone meta ...	1992	1317582
cyclic nucleotide-dependent regulation of agonist-induced calcium increases in mouse megakaryocytes.	1. the regulatory effects of cyclic amp and cyclic gmp on adp- and thrombin-induced increases in [ca2+]i were studied in mouse bone marrow megakaryocytes. changes in [ca2+]i were continuously monitored in single fura-2-loaded cells using microspectrofluorometry, and cyclic nucleotides were directly introduced into the single cells using the whole-cell patch-clamp technique. 2. adp increased [ca2+]i in a concentration-dependent fashion, and its threshold concentration was in the order of 0.01 mic ...	1992	1317440
the occurrence of periodontitis-related microorganisms in relation to titanium implants.	the peri-implant space was examined for the presence of periodontitis-related microorganisms in 19 patients. all patients had been fitted with osseointegrated titanium implants which were clinically sound at the time of examination and all had been prescribed daily 0.2% chlorhexidine mouthrinse. in 17 patients, two contralateral fixtures in patients were selected, the remaining 2 patients had only one fixture, and associated mucosal clinical variables assessed for plaque accumulation, gingival i ...	1992	1317427
physical map locations of the trxb, htrd, cydc, and cydd genes of escherichia coli.		1992	1317384
cloning, sequencing, and transcriptional regulation of viua, the gene encoding the ferric vibriobactin receptor of vibrio cholerae.	a 74-kda iron-regulated outer membrane protein of vibrio cholerae acts as the receptor for the v. cholerae iron-siderophore complex, ferric vibriobactin. mbg14, a mutant of v. cholerae 0395 containing a tnphoa insertion in a gene designated viua, lacks this 74-kda outer membrane protein and is unable to bind or utilize exogenous ferric vibriobactin. introduction of a plasmid containing the complete viua coding sequence and 513 bp of upstream dna into mbg14 restored ferric vibriobactin utilizatio ...	1992	1317381
a possible role of free radicals in the transplantation of retinal pigment epithelial cells.	oxygen-derived free radicals have been implicated in tissue injury following ischemia and reperfusion (reoxygenation). it has been hypothesized that the radicals are produced during the early reperfusion stage. recently, submacular implantation of retinal pigment epithelium cells has been reported. it is probable that during the procedure, the transplant and the 180-degree folded outer retina underwent a period of ischemia, followed by reoxygenation. we, therefore, infer that free radicals were ...	2005	1317028
kappa-opioid receptor stimulation abolishes mu- but not delta-mediated inhibitory control of spinal met-enkephalin release.	the possible opioid control through delta, mu and kappa receptors of the spinal release of met-enkephalin-like material (melm) was investigated in halothane-anaesthetized rats. the intrathecal perfusion of the delta agonist dtlet (10 microm) or the mu agonist dago (10 microm) resulted in a marked inhibition of melm release, which could be prevented by the selective antagonists naltrindole and naloxone, respectively. although the kappa agonist u 50488 h (10 microm) was inactive per se, it complet ...	1992	1317026
[prevalence of hepatitis c virus infection in a population of drug addicts and their heterosexual partners].	this study reports data concerning the prevalence of anti hcv in a population of 166 drug addicts and 25 partners (14 drug addicts and 11 with no history of substance abuse) of subjects proven anti hcv positive. the study was carried out in order to evaluate the importance of the haematic and sexual modes of transmission of hcv. the results obtained showed a prevalence of hcv infection in drug addicts with substantial statistic significance when compared with the prevalence in a population of do ...	1992	1317020
expression of yeast cytochrome c1 is controlled at the transcriptional level by glucose, oxygen and haem.	the nuclear gene for cytochrome c1 in saccharomyces cerevisiae (cyt1) was localized on chromosome xv. its upstream region was identified by functional complementation. fusion to the lacz reporter gene on a cen plasmid allowed study of the effect of carbon sources and of specific deletion mutations on expression of the gene in yeast transformants. detailed promoter analysis combined with expression studies in recipient strains defective in regulatory genes identified cis-acting sites and transcri ...	1992	1316998
effects of professional and media warnings about the association between aspirin use in children and reye's syndrome.	the media played a central role in changing the use of aspirin among children with viral illness following reports of its association with a rare but deadly disease, reye's syndrome (rs). it did so by alerting health professionals and parents about ways to prevent rs in children. indeed, by the time aspirin product labeling was required by the fda in 1986, most of the decline in rs incidence had already occurred. in the past, media-only health education campaigns have been relatively unsuccessfu ...	2005	1316991
leukotriene b4 omega-oxidation by human polymorphonuclear leukocytes is inhibited by pyocyanin, a phenazine derivative produced by pseudomonas aeruginosa.	human polymorphonuclear leukocytes (pmnl) metabolize the potent chemotaxin leukotriene b4 (ltb4) by omega-oxidation to 20-hydroxyl-ltb4 and 20-carboxy-ltb4. the ability of unstimulated human pmnl to metabolize exogenous ltb4 was found to be inhibited by pyocyanin, a phenazine derivative produced by pseudomonas aeruginosa, in a dose-dependent manner. 1-hydroxyphenazine (1-ohp), a metabolite of pyocyanin, was not inhibitory under identical conditions. the initial enzymic step in the conversion of ...	1992	1316878
identification of the vibriobactin receptor of vibrio cholerae.	vibrio cholerae produces the novel phenolate siderophore vibriobactin and several outer membrane proteins in response to iron starvation. to determine whether any of these iron-regulated outer membrane proteins serves as the receptor for vibriobactin, the classical v. cholerae strain 0395 was mutagenized by using tnphoa, and iron-regulated fusions were analyzed for vibriobactin transport. one mutant, mbg14, was unable to bind or utilize exogenous vibriobactin and did not grow in low-iron medium. ...	1992	1315733
rapid epidemiologic analysis of cytomegalovirus by using polymerase chain reaction amplification of the l-s junction region.	a technique based on polymerase chain reaction (pcr) amplification was developed to facilitate the study of the epidemiology of cytomegalovirus (cmv). consensus oligonucleotide primers from repetitive dna sequences were designed to amplify interspersed repetitive sequences in an area of heterogeneity within the l-s junction region of the cmv genome, and pcr products were detected by gel electrophoresis. purified cmv dnas from 25 cmv isolates, 13 from members of five families in which person-to-p ...	1992	1315336
the fortuitous diagnosis of cholera in a two-year-old girl.	we are reporting the fortuitous diagnosis of a case of cholera and the unusual failure of the commercial bacteriologic media that led to the unexpected isolation of vibrio cholerae. the case demonstrates the need for communication between the medical staff and laboratory personnel when an uncommon disease, such as cholera, is suspected. this case also alerts the clinician to the possibility of multiple enteric pathogens coinfecting a traveller.	1992	1315232
a serosurvey of pathogens associated with shellfish: prevalence of antibodies to vibrio species and norwalk virus in the chesapeake bay region.	recent concerns regarding the safety of shellfish consumption have focused on the risk posed by naturally occurring marine bacteria such as vibrio species and by viruses such as norwalk and related agents. despite the widespread environmental presence of vibrio species in the chesapeake bay, the rate of reported infections remains low; there have also been no reports of major norwalk outbreaks associated with shellfish in this area. as infections with these agents may not always be recognized be ...	1992	1312771
inhibin and inhibin alpha-chain precursors are produced by immature rat sertoli cells in culture.	stimulation of sertoli cells from immature rats with dibutyryl cyclic (dbc) amp resulted in a decrease in the ratio of inhibin biological (b):immunological (i) activities in vitro. to establish the basis for this decrease, culture medium from sertoli cells stimulated with dbcamp was fractionated by dye-affinity chromatography, reverse-phase hplc, and preparative page. two peaks of inhibin activity were identified: a predominantly bioactive 29-kda peak i material (b:i ratio = 5.0) and a bio-inact ...	1992	1312362
in situ hybridization of calcium/calmodulin dependent protein kinase ii and tau mrnas; species differences and relative preservation in alzheimer's disease.	abnormal phosphorylation of the microtubule associated protein tau component of neurofibrillary tangles (nfts) in alzheimer's disease (ad) may result from alterations in protein kinase expression. calcium/calmodulin dependent protein kinase ii (cam kinase ii) has been shown to phosphorylate tau in vitro in such a way to decrease its electrophoretic mobility. a68, apparently a modified form of tau in ad brain, also shows abnormal phosphorylation and slower mobility than tau. to further examine th ...	1992	1312209
regulation of interleukin-1 receptors on att-20 mouse pituitary tumour cells.	to study the cellular mechanisms of interleukin-1 (il-1) in the pituitary corticotroph, we studied the properties of il-1 receptors on a mouse pituitary acth-producing cell line, att-20. scatchard plot analysis revealed a single type of receptor with a kd (dissociation constant) of 93 pm, and 482 binding sites/cell. [125i]il-1 alpha binding competed with il-1 alpha and il-1 beta in an equimolar fashion. a 24 h pre-incubation with either crh, epinephrine or nor-epinephrine increased the [125i]il- ...	1992	1312014
regulation of interleukin-1 receptors on att-20 mouse pituitary tumour cells.	to study the cellular mechanisms of interleukin-1 (il-1) in the pituitary corticotroph, we studied the properties of il-1 receptors on a mouse pituitary acth-producing cell line, att-20. scatchard plot analysis revealed a single type of receptor with a kd (dissociation constant) of 93 pm, and 482 binding sites/cell. [125i]il-1 alpha binding competed with il-1 alpha and il-1 beta in an equimolar fashion. a 24 h pre-incubation with either crh, epinephrine or nor-epinephrine increased the [125i]il- ...	1992	1312014
non-o1 vibrio cholerae nrt36s produces a polysaccharide capsule that determines colony morphology, serum resistance, and virulence in mice.	non-o1 vibrio cholerae produced two distinct colony types, designated as opaque and translucent. nrt36s, a clinical isolate shown to be virulent in volunteers, produced predominantly opaque colonies, but translucent colonies appeared on subculture. opaque variants were recovered exclusively following exposure to normal human serum or animal passage. a nonreverting translucent mutant of nrt36s, jvb52, was isolated following mutagenesis with the transposon tn5 is50l::phoa (tnphoa). only translucen ...	1992	1311706
inhibitory role of gamma-amino-butyric acid in the rabbit urinary bladder.	gamma-amino-butyric acid (gaba) is an established inhibitory neurotransmitter in the central nervous system (cns) and it has also been identified in the bladder. we have investigated in the rabbit the effect of gaba on detrusor activity. rabbit detrusor muscle strips were made to contract by electrical stimulation of their autonomic nerves or by the addition of carbachol. the addition of gaba caused substantial inhibition of muscle contraction. gaba acts on 2 classes of receptors-gabaa and gabab ...	1992	1310630
carcinoma of the male breast metastatic to the mandible.	a case is reported of male breast carcinoma which, among other bony sites, metastasized to the mandible. this case and the review of the literature illustrate the need to include the mandible in bone scans for metastatic disease in breast cancer.	2005	1310607
[a chemical necrosis].		2005	1308898
an in vitro analysis of a sustained release system for the treatment of denture stomatitis.	this study investigated the feasibility of a sustained-release delivery system for the treatment of denture stomatitis using four antifungal agents (chlorhexidine, clotrimazole, fluconazole, and nystatin) incorporated into a tissue conditioner (lynal) at zero (control), low, medium, and high concentrations. hardness of all experimental groups increased with both concentration and time, but remained within the range of clinical acceptability (shore a < or = 49). mean tensile strength increased in ...	1992	1308323
tetracycline resistant el tor vibrios in loni area.		1992	1308032
[isolation of vibrio cholerae no-01 in the gulf of nicoya, costa rica].	in a bacteriological study on samples of bivalves, mud and surface waters from the gulf of nicoya, costa rica, 18 strains of non-01 vibrio cholerae and 50 of v. mimicus were isolated. the samples were enriched in alkaline peptone water, and streaked on macconkey and inositol-brilliant green bile agars. biochemical and serological tests were used for their identification. both species were isolated from all sampling sites (lepanto, jicaral and puntarenas) with either of the two agar media, even t ...	1992	1307117
demonstration of atrioventricular dissociation due to complete heart block by pulsed doppler echocardiography of hepatic veins.		2005	1307094
comparison of dental dimensions between rhinopithecus bieti and rhinopithecus roxellanae.		1992	1307079
[rheumatic manifestations of acquired immunodeficiency syndrome (aids)].	120 aids patients (mean age 33 +/- 9 years, 108 males) were evaluated regarding rheumatic manifestations. according to cdc's classification, 18.3% belonged to group ii, 28.3% to group iii, and 53.4% to group iv. arthralgia was present in 33 patients (27.5%), and in only 8 could be associated with infections other than hiv (5 cases of tuberculosis, 3 p. carinii, and 1 gonococcal infection). incidence of arthralgia was equal in either sex. arthritis was present in 8 patients, 2 of them with reiter ...	1992	1307073
[medical records--ethical aspects].		2005	1307070
[the chemical sensitivity of neurons in the medial vestibular nucleus to regulator peptides].		1992	1307049
study of lipid changes in freeze-dried fish during storage. i. the interaction of relative humidity and tissue lipids.	several studies on lipid stability of freeze-dried fish pointed out the relationship between relative humidity (rh) and autoxidation of highly unsaturated fatty acids. the present study shows the changes of tissue lipids in two different fish, freeze-dried and stored under various rh conditions. fillets of scomber scomber l and sardina pilchardus sardina were freeze-dried and stored for 1, 3, 6 and 9 months. periodically, the sample of both fish were analyzed to evaluate the autoxidation of lipi ...	2005	1307019
cytochrome p450-dependent metabolism of dextromethorphan: fetal and adult studies.	dextromethorphan undergoes o-demethylation to dextrorphan and n-demethylation to 3-methoxymorphinan. 3-hydroxymorphinan, a didemethylated compound, is secondarily formed. the o-demethylation pathway to dextrorphan is polymorphic and under cyp2d6 genetic control. adult and fetal studies were performed to characterize the cytochrome p450 families involved in dextromethorphan metabolism and their ontogeny. in adult volunteers in vivo and in vitro studies demonstrate that the o-demethylation pathway ...	1992	1306804
hypoxic-ischemic damage in the neonatal brain: excitatory amino acids.	perinatal brain damage is a major clinical problem. recent studies suggest that excitatory amino acids (eaas) may be important for the development of hypoxic-ischemic brain injury in the newborn. experimental work demonstrates that the immature brain is hypersensitive to the toxic effects eaa ('excitotoxicity'), hypoxic-ischemia is accompanied by an extracellular overflow of eaas and hypoxic-ischemic brain damage is reduced by eaa receptor antagonists. clinical investigations demonstrate the pre ...	1992	1306803
expression and release of neuroregulators during development: monoamines and neuropeptides.	the turnover of catecholamines (ca) was determined in the adrenal medulla and brain of rat fetuses and pups. in general we found a considerable increase soon after birth. the expression of mrna for ca-synthesizing enzymes was also considerably enhanced in the adrenals shortly after birth. furthermore, we demonstrated increased expression of neuropeptides after birth, increased synthesis of mrna encoding for neuropeptide y in the adrenals 24 h after birth; and considerable activation of the subst ...	1992	1306802
[in vivo and in vitro antitumoral effects of an extract from vibrio cholerae in different murine models. ii. antitumoral effect in vitro of an extract from vibrio cholerae: inhibition of 3ll cell growth and stimulation of murine splenocytes].	a bacterial extract from vibrio cholerae, the dgz, has been separated into two fractions by gel filtration. the effects of these two fractions and that of the crude extract are investigated and compared by two different in vitro tests. it appears that these extracts exert direct inhibition over the growth of 3ll cultured cells and that they trigger splenocytes in vitro proliferation.	1992	1305896
[a midwife in paraguay--indian children].		2005	1305231
immunomodulation of low dose streptozocin diabetes in mice reveals that insulitis is not obligatory for b cell destruction.	twenty male c57bl/j mice were injected with 50 microliters complete freund's adjuvant (cfa) 1 wk before and 1 wk after induction of diabetes with 45 mg streptozocin (stz)/kg body weight i.p. over 5 d. cfa administration prevented islet infiltration. inflammatory cells were not seen within any of the islets observed. however, islet b cell destruction still occurred. these cells showed evidence of considerable damage, containing swollen mitochondria, contracted nuclei and areas of vacuolation and ...	2004	1304577
tests of pulmonary function--i.		2004	1304271
[leu31-pro34] neuropeptide y identifies a subtype of 125i-labeled peptide yy binding sites in the rat brain.	subtypes of the neuropeptide y (npy) receptor in the rat brain were identified by the use of the selective y-1 analog, [leu34-pro34] npy. in rat brain homogenate binding studies, [leu31-pro34] npy was found to produce a partial inhibition of 100 pm 125i-labeled peptide yy (pyy) binding with a plateau at 50-1000 nm [leu31-pro34] npy resulting in a 70% inhibition of binding. the c-terminal fragment npy 13-36, a putative y-2 agonist, exhibited very little selectivity in rat brain homogenates. scatc ...	1992	1303142
[a guide for the study and treatment of the cadaver donor of organs for transplant].		2003	1302735
[the evaluation of the vibriocidal antibody test in establishing a diagnosis of cholera or vibrio carriage].	on the basis of the serological survey of cholera patients, vibrio carriers and persons having had contacts with the source or reservoir of vibrio cholerae the conclusion has been made that the test for the presence of vibriocidal antibodies, together with the bacteriological study of the patient, is of diagnostic importance in the diagnosis of cholera or vibrio carriership. the detection of vibriocidal antibodies, especially in the study of paired sera, permits the detection of cholera cases wh ...	1992	1301665
[in vivo and in vitro antitumoral effects of an extract from vibrio cholerae in different murine models. i. effect in vivo of bacterial extract from vibrio cholerae on the development of different murine tumoral grafts].	a bacterial extract from vibrio cholerae, so called dgz, is shown to prevent transplantation of sarcoma 180 and lewis lung carcinoma. a 4 days dgz treatment (4 x 100 micrograms) triggers reject of the graft by 40% of the mice. the mice which have rejected a graft once after treatment, cannot be grafted lately or, at least, the tumor growth is delayed. the association with cisplatinum (4 x 25 micrograms) allows to protect 90 to 95% of the mice.	1992	1301227
[dynamics of drug resistance of vibrio cholerae isolated from surface water reservoirs in siberia and the soviet far east in 1976-1990].	in the study on antibiotic resistance 1383 strains of el tor vibrio cholerae isolated from surface water reservoirs in 12 administrative territories of the siberia and far east within a period of 15 years were tested. the following antibiotics were used: ampicillin, streptomycin, monomycin, polymyxin, tetracycline, chloramphenicol, rifampicin and nalidixic acid. the resistance was unstable and its pattern was wave-like according to annual changes in the biological cycle. it was especially eviden ...	1992	1300926
non-o1 vibrio cholerae enterocolitis in quebec tourists returning from the dominican republic.		1992	1299428
diabetes in time of armed conflict: the croatian experience.	during the summer of 1991, armed conflict broke out in the republic of croatia. this resulted in many deaths and the displacement of many persons, with population loss in some communities and additional population burden in others. registration of diabetic persons has been compulsory in croatia since 1975 and from available statistics compiled by the vuk vrhovac institute for diabetes in zagreb it was known that in 1991 there were 111,096 diabetic persons in croatia, 16% requiring insulin, 46% t ...	2004	1299072
lose weight and win: a church-based weight loss program for blood pressure control among black women.	the baltimore church high blood pressure program (chbpp) offers a behaviorally oriented weight control program consisting of eight weekly 2-h diet counseling/exercise sessions. pre- and post-program weight and blood pressure measurements were analyzed for 184 black and 3 white women aged 18-81 years (mean 51) who participated in the program in 1984-1986: 88 were taking antihypertensive medication (rx) and 99 were not (no rx). mean weight loss was 6 lb in both groups: -18 to +7 lb in the rx group ...	1992	1298945
lose weight and win: a church-based weight loss program for blood pressure control among black women.	the baltimore church high blood pressure program (chbpp) offers a behaviorally oriented weight control program consisting of eight weekly 2-h diet counseling/exercise sessions. pre- and post-program weight and blood pressure measurements were analyzed for 184 black and 3 white women aged 18-81 years (mean 51) who participated in the program in 1984-1986: 88 were taking antihypertensive medication (rx) and 99 were not (no rx). mean weight loss was 6 lb in both groups: -18 to +7 lb in the rx group ...	1992	1298945
[rett's syndrome: description of a case with abnormal respiratory pattern].	rett' syndrome is a progressive disorder that occurs in females and is characterized by autistic behavior, dementia, ataxia, loss of purposeful use of the hands, and seizures. patients with rett' syndrome have been observed to have stereotyped hand movements (hand-washing) and to exhibit intermittent hyperventilation. to characterize more precisely the respiratory pattern associated with this disorder, sao2 and ph studies were made during sleep and wakefulness in a girl with this syndrome. these ...	2005	1298942
[rett's syndrome: description of a case with abnormal respiratory pattern].	rett' syndrome is a progressive disorder that occurs in females and is characterized by autistic behavior, dementia, ataxia, loss of purposeful use of the hands, and seizures. patients with rett' syndrome have been observed to have stereotyped hand movements (hand-washing) and to exhibit intermittent hyperventilation. to characterize more precisely the respiratory pattern associated with this disorder, sao2 and ph studies were made during sleep and wakefulness in a girl with this syndrome. these ...	2005	1298942
[hemolysin from vibrio cholerae eltor: cloning and expression of genes in escherichia coli].	a 6.56-kb v. cholerae eltor dna fragment encoding hemolysin synthesis was cloned in puc18. the resultant recombinant plasmid pes4h (9.25 kb) was mapped by restriction analysis and shown to express in different e. coli strains as well as in nonhemolytic v. cholerae strains. application of the cloned fragment as a molecular probe revealed homologous sequences in all v. cholerae strains tested independently on their biotypes, hemolytic activity and presence of vct-genes in their genomes while none ...	1992	1298873
hemolytic properties of some aeromonas strains.	considering the possible correlation between hemolytic and enterotoxigenic properties of aeromonas strains mentioned in the literature, in the present work we studied the practical value of the hemolysis tests in the diagnosis of aeromonas strains by using comparatively the hemolysis tube tests (with goat and sheep erythrocytes suspensions) as well as the technique on blood agar in aerobic conditions. there were studied comparatively 230 aeromonas strains (different species: a. hydrophila, a. so ...	1992	1297462
[a strain of aeromonas veronii isolated from a patient suspected of cholera].	investigations were carried on a strain isolated from patient hospitalized in poland. the patient returned from odessa, a region with cholera epidemic. the strain was suspected to be vibrio cholerae, but it did not agglutinate in v. cholerae 01 serum. the aim of this study was establishment of taxonomical classification of this strain, in order to demonstrate whether it is a representative of the non-cholera vibrio group (not agglutinating) or another representative of the family vibrionaceae. t ...	1992	1297030
isolation of enterotoxigenic vibrio cholerae non-01 from the buriganga river and two ponds of dhaka, bangladesh.	vibrio cholerae 01 is usually considered the most toxigenic member of the vibrionaceae and v. cholerae non-01 isolated from the environment is non-toxigenic. in our survey of the pollution of some aquatic environments in and around dhaka, bangladesh, we wanted to investigate the toxigenicity of v. cholerae non-01 isolated from water and sediment samples of the buriganga river and two ponds in dhaka, in the rabbit ileal loop (ril) model. fluid accumulation was induced by 18 of 28 live cultures an ...	1992	1296941
[the first cholera case diagnosed early in the clinical laboratory by dna probe method].	an alkaline-phosphatase-labelled synthetic oligonucleotide probe was applied to detect the structural gene of cholera enterotoxin (ctx). this dna probe has a complementary base sequence to 30 base of the ct-a subunit. this method was, for the first time, applied to diagnosis of a diarrheal patient. the probe detected ctx rapidly and simply as compared with reversed passive latex agglutination (rpla) and beads-elisa. the cfu minimal dose for detection with the probe was about 10(6-7)/ml. this met ...	1992	1294670
features of cholera and vibrio parahaemolyticus diarrhoea endemicity in calabar, nigeria.	the clinical and epidemiological features of acute vibrio diarrhoeal disease were studied in 881 patients seen at the university of calabar teaching hospital (ucth), calabar, nigeria, between january and december 1989. stools and rectal swabs of patients and randomly-selected control subjects were microscopically and culturally examined for the presence of enteric pathogens. households of vibrio diarrhoea cases and matched controls were visited for ecologic studies. of a total of 108 (12.3%) cul ...	1992	1294392
a common source foodborne outbreak of e1 tor cholera following the consumption of uncooked beef.	in july 1988, an outbreak of cholera with 71 culture-confirmed cases of biotype el tor, serotype ogawa, occurred in a non-endemic area in mae sot district, tak province. fifty-two cases had diarrhea and 19 had asymptomatic cholera infection. no cases died. epidemiological investigation revealed a significant association between cholera infection and the consumption of uncooked beef. increased risk of cholera infection was observed when the incriminated beef was kept at room temperature and consu ...	1992	1293258
[bacteriological study of traveller's diarrhoea. 4) isolation of enteropathogenic bacteria from patients with traveller's diarrhoea at osaka airport quarantine station during 1984-1991].	during the last 8 years (1984 to 1991), 16,639,233 overseas travellers were quarantined at osaka airport quarantine station and 38,326 travellers reported that they were (or had been) suffering from diarrhoea. bacteriological examination of stools from 12,573 persons revealed the following results. 1) various enteropathogenic bacteria were isolated from 3,669 cases (29.2%) examined. the predominant species of bacteria isolated were as follows: salmonella, 1049 cases; plesiomonas shigelloides, 10 ...	1992	1293222
effect of vibrio cholerae-l-asparaginase on surface structure of splenic lymphocytes.	the surface ultrastructure of splenic lymphocytes and rosetting properties of lymphocytes of swiss mice were studied under scanning electron microscope following transplantation of dalton's lymphoma and ascites fibrosarcoma tumour cells and administration of vibrio cholerae-l-asparaginase. the results were compared to those obtained with the standard escherichia coli-l-asparaginase. the surface structure of the lymphocytes (t cells) following l-asparaginase administration was not so different fr ...	1992	1291463
living with lasers.		2005	1291351
[stable and efficient expression of human interleukin-6 cdna in mammalian cells after gene transfer].	following reverse transcription and pcr amplification, the human interleukin-6 (il-6) cdna was cloned from total rna of activated human tonsillar mononuclear cells. southern blot showed the presence of specific band, and its dna sequence demonstrated that the fragment was 650 bp in length, spanning the complete coding region and part of 5' and 3'-untranslated regions. the human il-6 sequence seemed to be well conserved. one nucleotide change at 429 position was observed, but this change did not ...	1992	1291290
[immunotherapy of human retinoblastoma with rgntf-monoclonal antibody using nude mice as model].	retinoblastoma in nude mice as an animal model was developed by injecting cultured human retinoblastoma cell line(rb) y79 into the anterior chamber of eyeball. it was treated by direct injection of rgntf-mcab into the rb tumor. ten out of 30 eyeballs with rb inoculation did not develop tumor (32% inhibition) if rb cell suspension was mixed with the rgntf-mcab before injection; whereas in the control, all 40 but 3 eyeballs developed tumor (7%) when rb cell suspension alone had been injected. this ...	1992	1291287
feeling good affects your work. a team approach to health and productivity.		2005	1291285
getting your point across. communication wins over patients.		2005	1291279
dental school provisions for hiv-positive patients: should students have a choice?		1992	1291258
cholera associated with international travel, 1992--united states.		1992	1291009
recent progress in cholera vaccination.	cholera disease remains an important cause of morbidity and mortality in the third world. the parenteral cholera vaccine actually used offers only a 50% protection during 6 months. as vibrio cholerae and its toxin don't cross the gut wall, the aim of new vaccines is to prevent the colonization and growth of the vibrio in the jejuno-ileum and to inhibit the fixation of cholera toxin (ct) on its enterocyte membrane receptor. this can be afforded by stimulation of the gut local immune system mainly ...	1992	1288041
shape discrimination and the judgement of perfect symmetry: dissociation of shape from size.	we measured the accuracy with which subjects judged that a square or circle was perfectly symmetrical i.e. that aspect ratio (a/b) was exactly unity (where a and b were, respectively, the vertical and horizontal dimensions). errors were remarkably small, ranging from 0.7 to 0.4% for the judgement of squareness and from 1.4 to < 0.1% for the judgement of circularity. precision in judging aspect ratio was measured by requiring subjects to judge whether the aspect ratio (a/b)test of a test rectangl ...	2005	1287983
specific chemotherapy of chagas disease: a comparison between the response in patients and experimental animals inoculated with the same strains.	eleven strains of trypanosoma cruzi were isolated from patients with chagas disease in central brazil by xenodiagnosis and inoculation into newborn mice. biological characterization and isoenzyme analysis showed that 6 strains were type ii (zymodeme 2) and 5 were type iii (zymodeme 1). patients were treated with benznidazole or benznidazole plus nifurtimox. mice infected with each isolated strain were treated for comparison with the results obtained in the respective patient. evaluation of cure ...	1992	1287919
delayed cerebellar ataxia following falciparum malaria: lack of evidence for antibody mediation.		1992	1287911
[respiratory inductive plethysmography in the functional evaluation of patients undergoing coronary by-pass surgery].	the functional respiratory changes following coronary by-pass surgery were previously evaluated with the traditional spirometric method. aim of this work is to introduce the respiratory inductive plethismography (rip), a well known non-invasive technique, into the study of those volumetric and ventilatory modifications. eight cardioischemic patients were selected: all but one were free from pulmonary disorders. the tidal volume, the respiratory rate, the minute ventilation, the vital capacity an ...	2005	1287752
changes in burying behavior during the estrous cycle: effect of estrogen and progesterone.	this study demonstrates changes in cumulative burying behavior during various phases of the rat estrous cycle. low levels of burying behavior were observed during late proestrus. similar burying behavior levels among females tested in early proestrus, metestrus or diestrus and ovariectomized females were found. in ovariectomized females, treatment with estrogen (e) produced a slight, non-significant reduction in burying behavior. in contrast, progesterone (p) treatment resulted in a dose-depende ...	1992	1287686
cytotoxic and cytostatic effects of antitumor agents induced at the plasma membrane level.	a variety of antitumor agents inhibit cell proliferation by interacting with the plasma membrane. they act as growth factor antagonists, growth factor receptor blockers, interfere with mitogenic signal transduction or exert direct cytotoxic effects. the p-glycoprotein encoded by the mdr1 gene represents a transmembrane protein which catalyzes the efflux of various antitumor agents. this membrane protein is the target of compounds acting as multi-drug resistance (mdr)-modulators. finally, several ...	2005	1287673
relationships between sequence and structure for the four-alpha-helix bundle tertiary motif in proteins.	the sequences of four-alpha-helical bundle proteins are characterized by a pattern of hydrophilic and hydrophobic amino acids which is repeated every seven residues. at each position of the heptad repeat there are specific constraints on the amino acid properties which result from the topology of the tertiary motif. these constraints give rise to patterns of amino acid distribution which are distinct from those of other proteins. the distributions in each of the heptad positions have been determ ...	2005	1287654
prenatal diagnosis of distal arthrogryposis type i by ultrasonography.	two consecutive pregnancies in a woman with initially undiagnosed type i distal arthrogryposis (da) are reported. a prenatal diagnosis of the condition was made by ultrasound in the 17th week of gestation in one of the pregnancies, whereas in the subsequent pregnancy the disorder was excluded as early as 13 weeks' gestation. the diagnoses were verified at birth. the feasibility of prenatal diagnosis of da type i in the second trimester is thus confirmed and its possibility in the late first trim ...	2005	1287640
melanin standard method: titrimetric analysis.	melanin isolated from the ink sac of sepia officinalis (sepia melanin) has been proposed as a standard for natural eumelanin, and a standard mild isolation and purification protocol for sepia melanin has been developed (zeise, doctoral dissertation, johns hopkins university, 1991). the goal of the present work, developed using sepia melanin, was to quantify the bioavailable carboxylic acid groups present in melanin particles. bioavailability is governed by the accessibility of carboxy groups to ...	2005	1287626
[vibrio cholerae can be encountered even at home].		1991	1285425
travel-associated cholera.		1992	1285192
myelin specific, autoaggressive t cell clones in the normal immune repertoire: their nature and their regulation.		2005	1285063
[the agglutinability of cholera o-monoclonal immunoglobulins].	a set of 10 monoclonal antibodies specific for vibrio species and of 5 monoclonal antibodies specific for serovar (ogawa) was studied. these monoclonal antibodies were directed toward v. cholerae o1 antigen in agglutination reaction and on slide plates. monoclonal antibodies agglutinating typical strains of cholera vibrios with titration range from 1: 6000 to 1: 25,000 were selected. ma were revealed to interact with cholera vibrio strains with reduced agglutinability. the set of agglutinating o ...	1992	1284089
brain filament proteins in primary cultures derived from chick embryos early in development.	brain filament expression and neurofilament post-translational modifications (phosphorylations) were studied in primary cultures derived from whole 3-4 day chick embryos. after 2-3 days in culture, neurofilament-positive cells formed neuronal aggregates connected by bundles of neurites in a distinctive pattern similar to that observed in cultures derived from embryonal rat brain and neonatal rat cerebellum. aggregates and neuritic bundles were stained with several monoclonal antibodies reacting ...	1992	1283802
serological cross-reaction between intact and chemically modified lipopolysaccharides of o1 vibrio cholerae inaba and non-o1 v. cholerae bio-serogroup hakata.	serological cross-reaction of intact as well as chemically modified lps from o1 vibrio cholerae 569b (inaba) with non-o1 v. cholerae hakata lps, which contain alpha(1-->2)-linked n-acetyl perosamine-homopolymer constituting their o polysaccharide chain, was studied by passive hemolysis test by using their lps as antigen for sensitizing sheep red blood cells (srbc). the n-deacylation of the alpha(1-->2)-linked linear 3-deoxy-tetronyl perosamine-homopolymer constituting the o polysaccharide chain ...	1992	1283438
notes on a light and electron microscopic double-labeling method combining anterograde tracing with phaseolus vulgaris leucoagglutinin and retrograde tracing with cholera toxin subunit b.	investigations of monosynaptic connections in the central nervous system have been hindered by the lack of compatible markers that can be used at both light and electron microscopic levels. in attempts to determine synaptic contacts between fibers originating in the substantia nigra and neurons projecting to the spinal cord, we have developed a double immunolabeling technique using anterograde transport of phaseolus vulgaris leucoagglutinin (pha-l) and retrograde transport of unconjugated choler ...	1992	1283431
purification of aeromonas hydrophila major outer-membrane proteins: n-terminal sequence analysis and channel-forming properties.	four outer-membrane proteins of aeromonas hydrophila were purified and their n-terminal sequences and channel-forming properties were determined. three could be matched with proteins from other species. one was a maltoporin, as its level increased when cells were grown in maltose-containing media, and the channel it formed was blocked by maltose. another was like ompf and ompc of escherichia coli, except that its channel fluctuated much more rapidly. the third protein, which was produced in low- ...	1992	1283000
multi-recognition capability of e-selectin in a dynamic flow system, as evidenced by differential effects of sialidases and anti-carbohydrate antibodies on selectin-mediated cell adhesion at low vs. high wall shear stress: a preliminary note.	e-selectin has a "multi-recognition" capability in terms of epitope binding specificity, depending on adhesion conditions (static vs. low- or high-shear stress dynamic systems). specifically, (i) adhesion based on expression of alpha 2-->3 sialylated le(x) (sle(x)) is prominent under static or low shear stress dynamic conditions; (ii) adhesion under high shear stress dynamic conditions does not depend on the known sle(x) species, but rather on lex with an adjacent unidentified sialosyl substitut ...	1992	1282810
cell surface characteristics of environmental and clinical isolates of vibrio cholerae non-o1.	the cell surfaces of several toxigenic and nontoxigenic environmental and clinical isolates of vibrio cholerae non-o1 have been examined. the environmental strains, irrespective of toxigenicity, are significantly more resistant to antibiotics and detergents than are v. cholerae o1 strains. the clinical isolates of non-o1 vibrios are as sensitive to a wide variety of chemicals as the o1 vibrios. the environmental non-o1 strains are also less susceptible to lysis when treated with protein denatura ...	1992	1282793
sialyl ligands facilitate lymphocyte accumulation during inflammation of the central nervous system.	combined models of cytokine-induced inflammation in the skin and spinal cord of the rat were utilised to demonstrate in vivo that circulating lymphocytes depend upon sialylated adhesion molecules on their surface for maximal recruitment into inflammatory sites in both tissues. when radiolabelled normal spleen cells were incubated with sialidase from vibrio cholerae or clostridium perfringens, or with the specific sialic acid-binding lectin from limax flavus, prior to being washed and injected in ...	1992	1281823
[molecular cloning and expression of vibrio cholerae lps o-antigen genes in e. coli hb101].	the chromosomal fragments of vibrio cholerae classic biotype and eltor biotype were cloned respectively with vector plasmid puc18 and b.s(m13+). we got recombinants which could express the lps o-antigen of vibrio cholerae, and the expressed o-antigen expressed had very good specificity and immunogenicity. after analyzing the recombinant plasmids by restriction endonucleases digestion, we found the size of foreign fragments in pmg-301 and pmg-302 were 8.4 kb and 7.6 kb, which were much smaller th ...	1992	1281653
the structure of the o-antigenic polysaccharide from lipopolysaccharide of vibrio cholerae strain h11 (non-o1).	after acid degradation of the lipopolysaccharide (lps) of vibrio cholerae strain h11 (non-o1), a tetrasaccharide was obtained, the structure of which was determined by quantitative and methylation analyses, periodate oxidation, one- and two-dimensional nmr spectroscopy, and fast-atom-bombardment and four-sector tandem mass spectrometry as beta-d-galangro-(1-3)-beta-d-quinac-(1-4)-alpha-d-galangr o-(1-4)-neuac, in which galangro is n-galacturonoyl-2-aminoglycerol and quin 2-amino-2,6-dideoxy-gluc ...	1992	1281098
intelligibility assessment in developmental phonological disorders: accuracy of caregiver gloss.	fifteen caregivers each glossed a simultaneously videotaped and audiotaped sample of their child with speech delay engaged in conversation with a clinician. one of the authors generated a reference gloss for each sample, aided by (a) prior knowledge of the child's speech-language status and error patterns, (b) glosses from the child's clinician and the child's caregiver, (c) unlimited replays of the taped sample, and (d) the information gained from completing a narrow phonetic transcription of t ...	2005	1280309
[flow cytometric analysis of ki-67 reactive antigen].	monoclonal antibody ki-67 is a useful and easy means for evaluating cell proliferative activity. furthermore, it appears to provide valuable prognostic informations in some neoplastic diseases. however, almost all studies on solid tumors were performed only by microscopic observation using immunohistochemistry, and not by flow cytometry. therefore, the information deduced from the findings is limited. the method of dna/ki-67 double staining of solid tissue and cultured cells for flow cytometry i ...	2005	1280305
[experimental study of hyperostosis induced by hypervitaminosis a].	radiological and histological studies were made on retinoid-induced hyperostosis in rats. vitamin a (va) was administered intraperitoneally in rats for 6 months. hyperostosis was observed in 94 percent of rats administered va and in 38 percent of the control. chondrocytes and vascular proliferation were observed in the attachment of the tendons and in the anterior corner of the vertebral body after 3 months. hyperostosis was observed as osteophytes in the attachment of ligaments or tendons and a ...	1992	1280297
antagonism of 3,4-methylenedioxymethamphetamine-induced neurotoxicity in rat brain by 1-piperonylpiperazine.	the effects of 1-piperonylpiperazine and n,alpha-dimethylpiperonylamine, which are weak inhibitors for [3h]5-hydroxytryptamine (5-ht) uptake, on 3,4-methylenedioxymethamphetamine (mdma)-induced neurotoxicity were examined. the reductions of serotonergic parameters in the rat cerebral cortex produced by multiple administration of mdma (10 mg/kg) were attenuated significantly by coadministration of 6-nitroquipazine (10 mg/kg), paroxetine (10 mg/kg) or 1-piperonylpiperazine (20 mg/kg), but not by n ...	1992	1280228
cancer metastasis--a multistep process.	dermatologists are confronted daily with proliferative growths which can reach considerable size, but mean no danger to the patient. seborrheic keratoses are one example. other growths, such as basal cell carcinoma, are locally destructive. normally, however, we do not hesitate to reassure the patient about the benignity of the tumor once excised. the malignancy of squamous cell carcinomas and melanoma, however, is undoubted. thus, excessive proliferation and even local invasiveness are not suff ...	2005	1280176
vibrio cholerae non-o1 isolated from ayu fish (plecoglossus altivelis) in japan.	a fish pathogen, vibrio cholerae non-o1, was isolated from diseased ayu fish (plecoglossus altivelis) collected from rivers in eight prefectural districts of japan. this organism was found to have biochemical characteristics similar to those of v. cholerae non-o1, except that our isolates were negative for ornithine decarboxylase. antiserum against an ayu isolate did not agglutinate with the majority of environmental v. cholerae non-o1 isolates, but a major o antigen was common among the ayu iso ...	1992	1280062
neonatal tetanus: a developmental follow-up study.		2005	1278947