Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| a new specific, sensitive and non-carcinogenic reagent for the demonstration of horseradish peroxidase. | 1977 | 72746 | |
| afferents to the entorhinal cortex of the rat studied by the method of retrograde transport of horseradish peroxidase. | 1977 | 72679 | |
| somatopetal transport of horseradish peroxidase (hrp) in the peripheral and central branches of dorsal root ganglion cells. | the axonal transport of hrp in both the peripheral and central branches of dorsal root ganglion cells was studied in rats. for studying axonal transport in the peripheral branch hrp as a dry substance was applied to the peroneal nerve injured either by teasing, by cutting or crushing. after a short survival time (22 h) mainly small spinal ganglion cells of the corresponding segments were labelled, while after a prolonged survival time (70 h) mainly large cells were labelled. these labelling diff ... | 1977 | 72611 |
| an electron microscopic investigation of the surface coat of the electrocyte of electrophorus electricus. | the surface coat of the electrocyte of the main electric organ of electrophorus electricus was studied using cytochemical methods (periodic acid-silver methanamine, periodic acid-chromic acid-silver methenamine, periodic acid-thiosemicarbazide-silver proteinate, concanavalin a - horseradish peroxidase, ruthenium red, alcian-blue lanthanum nitrate, colloidal iron hydroxide and cationized ferritin). the surface of the electrocyte presents perpendicularly oriented tubular invaginations of the cell ... | 1977 | 72610 |
| cerebeller afferents from neurons in motor nuclei of cranial nerves demonstrated by retrograde axonal transport of horseradish peroxidase. | 1977 | 72590 | |
| salivatory neurons innervate the submandibular and sublingual glands in the rat: horseradish peroxidase study. | 1977 | 72589 | |
| the olivocerebellar projection in the cat studied with the method of retrograde axonal transport of horseradish peroxidase. vi. the projection onto longitudinal zones of the paramedian lobule. | microinjections (30-50 nl) of a horseradish peroxidase (hrp) suspension of 25% (wt./vol.) were made in different folia of the paramedian lobule of cats, and the sites of occurrence of labeled cells in the inferior olive were precisely determined. in each case only a small number of cells are labeled, aggregated in a minute area. the labeled cells are found within three only of the four olivary areas previously determined (brodal et al., '75) to project onto the paramedian lobule (fig. 1): one ar ... | 1977 | 72081 |
| the olivocerebellar projection studied with the method of retrograde axonal transport of horseradish peroxidase. v. the projections to the flocculonodular lobe and the paraflocculus in the rabbit. | hrp was injected in the flocculonodular lobe and the paraflocculus in the rabbit to determine the areas of the inferior olive which project onto these cerebellar regions. following injections in the flocculus labeled cells occurred in the dorsal cap and the rostralmost tip of the medial accessory olive. following injections in the nodulus labeled cells were likewise found in the dorsal cap, but in addition in the rostralmost part of the dorsomedial cell column and the adjoining part of the media ... | 1977 | 72080 |
| observations on labeling of neuronal cell bodies, axons, and terminals after injection of horseradish peroxidase into rat brain. | 1977 | 72079 | |
| detection of epstein-barr virus antigens with enzyme-conjugated antibody. | monospecific conjugated (fluorescein isothiocynate and horseradish peroxidase) goat antisera, prepared against three human immunoglobulin classes, im (mu), igg (gamma) and iga (alpha), were compared for their ability to detect human ig classes possessing specificity for epstein-barr virus (ebv) viral capsid antigens (vca) in a chronically infected human lymphoid cell line, p3j-hrik. it was determined that the enzyme system was significantly more sensitive than immunofluorescence in detecting mos ... | 1977 | 72045 |
| the pontine projection to the crebellar anterior lobe. an experimental study in the cat with retrograde transport of horseradish peroxidase. | 1977 | 72000 | |
| arborization of initial axon collaterals of spinocervical tract cells stained intracellularly with horseradish peroxidase. | the initial axon collateral arborizations of two feline spinocervical tract cells have been analysed after intracellular staining with horseradish peroxidase. the images of the cells were reconstructed in the light microscope from transverse serial sections. the cell bodies were situated in laminae iv or iii of the lumbar dorsal horn. each cell exhibited one initial collateral. these emerged from the parent axons at distances of about 310 and 580 micron, respectively, from the cell body. the col ... | 1977 | 71934 |
| the permeability of the human ciliary and iridial epithelium to horseradish peroxidase. an in vitro study. | the permeability of the human ciliary epithelium to horseradish peroxidase (po) has been studied in vitro with the electron microscope. ciliary body and iris specimens were obtained from freshly enucleated eyes. po was applied at the stromal side of the epithelium, and was left for 120 min. the movement of po through the intercellular spaces of the human ciliary epithelium was blocked apically in the lateral intercellular spaces of the non-pigmented epithelial cells, indicating that these cells ... | 1977 | 71845 |
| movement of horseradish peroxidase in the cornea, sclera and the anterior uvea. | the anterior chambers of rabbit, monkey and human eyes were perfused with horseradish peroxidase. the perfusion was started immediately after enucleation of the monkey and human eyes, while the rabbit eyes were perfused in situ after the animals had been killed. comparative results were obtained after 1 h or perfusion and were mainly based on frozen sections. intensive staining was found in the descemet's membrane, the sclera and the iridial stroma, considerably more than in the corneal stroma a ... | 1977 | 71844 |
| localization of thyroiglobulin antigenicity in rat thyroid sections using antibodies labeled with peroxidase or 125i-radioiodine. | in the hope of localizing thyroglobulin within focullar cells of the thyroid gland, antibodies raised against rat thyroglobulin were labeled with the enzyme horseradish peroxidase or with (125)i-radioiodine. sections of rat thyroids fixed in glutaraldehyde and embedded in glycol methacrylate or araldite were placed in contact with the labeled antibodies. the sites of antibody binding were detected by diaminobenzidine staining in the case of peroxidase labeling, and radioautography in the case of ... | 1977 | 71304 |
| origin of the direct hippocampus--anterior thalamic bundle in the rat: a combined horseradish peroxidase--golgi analysis. | 1977 | 71242 | |
| uptake of horseradish peroxidase during the cell cycle of hepatoma cells in culture. | 1976 | 71071 | |
| [projections of bulbar reticular nuclei and raphe nuclei to lobules vi and vii of cat cerebellar cortex]. | injections of horseradish peroxidase were made in lobules vi and vii of the cerebellar vermis (in the cat). serial frontal sections of the brain stem reveal that the lateral and paramedian nuclei were essentially projected onto lobule vi, and the projections from the raphe nuclei onto lobule vii. | 1977 | 70281 |
| retrograde axonal transport of horseradish peroxidase for determining motor projection patterns to the developing limb in xenopus. | horseradish peroxidase (hrp) injected into developing limb buds of xenopus laevis tadpoles is carried by retrograde axonal transport to the somata of motoneurones in the ventral horn. small injection of 10% hrp were found to remain well localised to specified sites in the limb bud. two types of labelled cells were found: diffusely labelled and granular labelled. diffusely labelled cells result from axonal damage in the presence of hrp. granular labelled cells result only from uptake of hrp from ... | 1977 | 70260 |
| role of gangliosides in the uptake and retrograde axonal transport of cholera and tetanus toxin as compared to nerve growth factor and wheat germ agglutinin. | previous investigations have shown that tetanus toxin is transported retrogradely in all peripheral neurons whereas the transport of ngf is confined to adrenergic and sensory neurons. other macromolecules with molecular weights and general physiochemical properties similar to ngf and tetanus toxin (e.g., cytochrome c, insulin, horseradish peroxidase and bovine serum albumin) are not transported to a detectable extent if injected in comparable molar concentrations. for tetanus toxin, which is tra ... | 1977 | 70259 |
| evidence for direct synaptic interconnections between cat spinal alpha-motoneurons via the recurrent axon collaterals: a morphological study using intracellular injection of horseradish peroxidase. | by using intracellular injection of horseradish peroxidase into cat triceps surae alpha-motoneurons we have been able to trace the motor axons as well as the recurrent motor axon collaterals with their synaptic terminals both with the light and electron microscope. in addition to the expected projection to the 'renshaw cell area', the triceps surae motor axon collaterals were frequently found to terminate within the motor nuclei, where at least some of the terminals made direct synaptic contact ... | 1977 | 70258 |
| bidirectional movement of horseradish peroxidase and the demonstration of reciprocal thalamocortical connections. | 1977 | 70257 | |
| axoplasmic transport in ocular hypotony and papilledema in the monkey. | orthograde and retrograde axoplasmic transport were studied in optic nerve heads of seven hypotensive macaca fascicularis eyes. orthograde transport was studied by radioautography after intravitreal radioisotope injections. retrograde transport was studied in the same eyes by horseradish peroxidase injection into the dorsal lateral geniculate nuclei or optic tracts. three eyes had developed marked papilledema before injections. orthograde axoplasmic transport was blocked in swollen axons of the ... | 1977 | 70200 |
| ultrastructural immunoenzymatic study of alpha-fetoprotein-producing cells in the human fetal liver. | cells producing alpha-fetoprotein in human fetal liver have been studied with specific horseradish peroxidase labeled immunoglobulins. under light microscopy, the alpha-fetoprotein is strictly localized in the cytoplasm of certain hepatocytes, distributed randomly in the hepatic lobule. ultrastructural examination of thesame cells shows that the alpha-fetoprotein is present within the cytoplasm. ultrastructural differences are described in hepatocytes according to whether or not the cell is prod ... | 1977 | 69654 |
| the effects of monocular deprivation on different neuronal classes in the lateral geniculate nucleus of the cat. | retrograde axonal transport of horseradish peroxidase (hrp) was used to identify two populations of cells in the lateral geniculate nucleus (lgn) of the cat. hrp was injected into area 17 and 18 separately in the same animals, and the neuronal somata giving rise to thalamo-cortical axons, identified by the presence of granular hrp reaction product within them, were measured. the mean size of lgn neurones labelled by injections in area 17 ("17-relay" cells) was less than of neurones filled from a ... | 1977 | 69546 |
| identification of cells of origin of tectopontine fibers in the cat superior colliculus: an experimental study with the horseradish peroxidase method. | the pontine projections from the superior colliculus in the cat have been studied by means of retrograde axonal transport of horseradish peroxidase (hrp). following injections of hrp in the dorsolateral pontine nucleus, where the tectopontine fibers terminate, a fair number of labeled cells are found throughout the rostrocaudal extent of the ipsilateral superior colliculus. relatively few of the labeled cells are of medium size (25-40 micron in diameter), more than 80% are small (10-25 micron), ... | 1977 | 69477 |
| ultrastructural localization of a nerve growth factor-horseradish peroxidase (ngf-hrp) coupling product after retrograde axonal transport in adrenergic neurons. | 1977 | 69476 | |
| characterization of the pigeon isthmo-tectal pathway by selective uptake and retrograde movement of radioactive compounds and by golgi-like horseradish peroxidase labeling. | 1977 | 69469 | |
| slow rate transport of horseradish peroxidase in toads. | 1977 | 69468 | |
| demonstration of bilateral claustro-cortical connections in the cat with the method of retrograde axonal transport of horseradish peroxidase. | the claustrum of the cat was studied to determine the extent of its projection to the cerebral cortex. neurons in the bilateral dorsal claustrum are labeled by retrograde axonal transport of horseradish peroxidase (hrp) from injection sites of different neocortical areas with preponderance to the ipsilateral side. it is revealed that the dorsal claustrum projects to almost entire regions of the bilateral neocortex and that distribution of labeled neurons in the bilateral claustrum has topographi ... | 1977 | 69423 |
| retrograde axonal transport of horseradish peroxidase. uptake at mouse neuromuscular junctions following systemic injection. | eye muscles were examined histochemically for the presence of horseradish peroxidase (hrp) activity after intravenous injections of large doses of this tracer protein. hrp had penetrated through the blood vessels and diffused into the areas of motor end plates, where it outlined axon terminals at the synaptic clefts. hrp was incorporated into pinocytotic vesicles in the axons from where an intraaxonal transport in the retrograde direction to the nerve cell bodies in the brainstem followed. accum ... | 1977 | 69382 |
| [study of the vagal innervation of the cat stomach by means of retrograde axoplasmic transport of horseradish peroxidase--on the localization of the origins of the preganglionic fibers]. | 1977 | 68782 | |
| commercial horseradish peroxidase degrades myelin encephalitogenic protein during coupling for immunohistochemical studies. | conjugates of myelin encephalitogenic basic protein (ep) and commercial horseradish peroxidase (hrp) have been used for immunohistochemical demonstrations of anti-ep antibody in animals with experimental allergic encephalomyelitis. we performed gel electrophoresis studies on ep-hrp conjugates prepared with glutaraldehyde and on mixtures of ep and hrp incubated without glutaraldehyde. the results show that under conditions of one-and two-step coupling hrp causes rapid loss of the native ep band, ... | 1977 | 68067 |
| the organization of thalamocortical relay neurons in the rat ventrobasal complex studied by the retrograde transport of horseradish peroxidase. | 1977 | 68039 | |
| a new and sensitive staining method for axonally transported horseradish peroxidase (hrp) in the pigeon visual system. | 1977 | 67876 | |
| identification of different size motoneurons labeled by the retrograde axonal transport of horseradish peroxidase. | three main groups of motoneurons of different size have been labeled in adult cats by using the method for retrograde axonal transport following injection of horseradish peroxidase in the medial gastrocnemius and soleus muscles. in particular small, medium-size and large neurons which probably correspond respectively to gamma, small alpha and large alpha motoneurons innervating the calf muscles, have been identified and the corresponding area measured. | 1977 | 67590 |
| orthograde and retrograde axoplasmic transport during acute ocular hypertension in the monkey. | orthograde and retrograde axoplasmic transport have been studied in the optic nerve heads of 37 macaca fascicularis eyes with normal or elevated intraocular pressure (iop) produced by cannulation of the anterior chamber. orthograde transport was labeled by 3h-amino acids injected intravitreally and incorporated into retinal ganglion cell proteins. retrograde transport was studied in the same eyes by injecting horseradish peroxidase (hrp) into one or both optic tracts and dorsal lateral geniculat ... | 1977 | 67096 |
| retrograde axonal transport of horseradish perioxidase in afferent fibers of the inferior mesenteric ganglion of the guinea pig. identification of the cells of origin in dorsal root ganglia. | 1977 | 66970 | |
| localization of abductor and adductor fibers in the kitten recurrent laryngeal nerve: use of a variation of the horseradish peroxidase tracer technique. | 1977 | 66152 | |
| brain stem projections to lobule vii of the posterior vermis in the squirrel monkey: as demonstrated by the retrograde axonal transport of tritiated horseradish peroxidase. | 1977 | 66085 | |
| organization of the facial nerve nucleus in the cat. | the location of efferent neurons in the brain stem of newborn kittens supplying the facial muscles was studied by means of horseradish peroxidase (hrp) used as a tracer of retrograde protein transport. three to 15 microliters containing 0.3 to 1.5 mg of hrp was injected into the orbicularis oris and orbicularis oculi muscles of six kittens and in the seventh kitten, the ventral ramus of the facial nerve was transected and hrp was instilled on the cut end of the nerve. after a survival time of 24 ... | 1977 | 65707 |
| ultrastructural visualization of cellular carbohydrate components by means of lectins on ultrathin glycol methacrylate sections. | a method for the visualization of cellular carbohydrate components by both light and electron microscopy using lectins on glycol methacrylate sections is proposed. this method, which is an application of the lectin-peroxidase affinity technique, solves the problem of limited penetration when it is attempted to demonstrated lectins receptors within the tissue block. following partial dissolution of glycol methacrylate from thin sections using alcohol, they are incubated successively with lectin ( ... | 1977 | 65417 |
| the vestibular complex of the american opossum didelphis virginiana. ii. afferent and efferent connections. | we have demonstrated the connectivity of the opossum's vestibular nuclei using degeneration, autoradiographic and horseradish peroxidase techniques and have found it to be generally comparable to that reported for the cat. apart from the primary input described in part i of our study, the cerebellum provides the major source of afferent connections to the vestibular complex. axons from the cerebellar cortex distribute mainly to vestibular areas which receive no primary afferent projections, e.g. ... | 1977 | 65367 |
| brain stem afferents to the fastigial nucleus in the cat demonstrated by transport of horseradish peroxidase. | although retrograde and anterograde degeneration studies have provided important information concerning brain stem afferents to the fastigal nucleus (fn), these data may be incomplete and should be confirmed by axonal transport methods. attempts were made to inject horseradish peroxidase (hrp) unilaterally into the fn in a series of adult cats. animals were perfused with dextran and a fixative solution of paraformaldehyde and glutaraldehyde in 0.1 m phospate buffer. representative sections were ... | 1977 | 65366 |
| the olivocerebellar projection in the cat studied with the method of retrograde axonal transport of horseradish peroxidase. iv. the projection to the anterior lobe. | following injections of horseradish peroxidase (hrp) in the cerebellar cortex of the anterior lobe of the cat, the distribution of labeled cells in the inferior olive was mapped. the findings largely confirm those made previously in studies of olivary retrograde cell loss following cerebellar ablations (brodal, '40b). in addition, they reveal further olivary areas projecting onto the anterior lobe, and permit a more detailed analysis of the pattern in this projection. concerning major points the ... | 1977 | 65365 |
| the organization of the facial nucleus of the brush-tailed possum (trichosurus vulpecula). | the facial nucleus of the brush-tailed possum has been studied using nissl staining and the horseradish peroxidase (hrp) retrograde tracing technique. in nissl stained sections the nucleus is seen to comprise five distinct subnuclei. injections of hrp into individual facial muscle groups have shown that these subnuclei reflect the peripheral innervation pattern of efferents from this nucleus. although in most cases, injection of hrp into a single facial muscle group resulted in the labelling of ... | 1977 | 65363 |
| efferent projections of the main and the accessory olfactory bulb in the tree shrew (tupaia glis). | the projections of the main and the accessory olfactory bulb in the tree shrew (tupaia glis) have been analyzed with anterograde degeneration and autoradiographic methods for identifying axonal projections, and with the horseradish peroxidase method for identifying the distribution of neurons from which these projections originate. the cytoarchitectonic features of the paleocortical areas which receive projections from the main and the accessory olfactory bulb have also been described. the effer ... | 1977 | 65361 |
| functional organization of the vestibular input to the anterior and posterior cerebellar vermis of cat. | 1. responses evoked by electrical stimulation (auditory division of the viiith nerve sectioned chronically) and natural stimulation of the vestibular apparatus were recorded in the anterior and posterior cerebellar vermis of cats anesthetized with ketamine or nembutal. under ketamine the functional state of the cerebellar cortex was similar to that of the decerebrate or encéphale isolé preparation. 2. vestibular-evoked responses were found bilaterally throughout the vermis (lob. i-x) and parts o ... | 1977 | 65291 |
| anatomical studies of a temporal visual area in the rabbit. | using fink-heimer, autoradiographic and horseradish peroxidase techniques, the connections of a temporal visual cortical region of the rabbit were explored. the temporal visual area covers portions of areas t1 and t2, and is reciprocally connected with the posterior nucleus and suprageniculate nuclei of the thalamus. it was also shown that the temporal visual area projects to a similar region in the opposite hemisphere, and to intermediate laminae of the superior colliculus. the temporal visual ... | 1977 | 64476 |
| identification of cells or origin of non-primary afferents to the dorsal column nuclei of the cat. | in order to identify cells of origin in the spinal cord of non-primary afferents to the dorsal column nuclei (dcn), the retrograde transport of horseradish peroxidase (hrp) has been utilized in adult cats. 10 to 30% hrp was injected bilaterally (0.6 mul per side) in the dorsal medulla of nine cats. in most instances the spread of the injected enzyme extended a few millimeters rostrocaudally and infiltrated the dcn as well as other nuclei and fiber tracts. labelled cells in these cases are numero ... | 1977 | 64365 |
| intraaxonal transport of horseradish peroxidase in the sympathetic nervous system. | following a single injection of horseradish peroxidase (hrp) into the superior cervical ganglion (scg) of the rabbit, the uptake and anterograde transport of this label was confirmed in the ganglion cell bodies, postganglionic axons, and preterminal and terminal ending axons in the ciliary processes of the eye. from the same injection site the intraaxonal hrp reaction product was demonstrated in myelinated axons, presumably by retrograde transport. intracytoplasmic hrp was identified in large, s ... | 1977 | 64283 |
| the blue reaction product in horseradish peroxidase neurohistochemistry: incubation parameters and visibility. | a blue reaction product is formed at sites that contain horseradish peroxidase (hrp) activity when benzidene is used as the chromogen. with neutral red as a counter stain, this method affords excellent visualization of both retrograde and orthograde axonal transport of intracerebrally injected hrp. the visibility of this blue reaction-product is better than the visibility of the brown reaction-product obtained in the commonly used diaminobenzidene procedures. variations in incubation times and r ... | 1976 | 63512 |
| the anatomical organization of the cerebello-olivary projection in the cat. | the cerebello-olivary pathway in the cat has been examined using orthograde and retrograde neuroanatomical tracing techniques. the orthograde transport of 3h-leucine from injection sites in the deep cerebellar nuclei labeled dentate and interpositus projections to the rostral two-thirds of the contralateral inferior olivary complex. these projections are topographically organized, with the dentate nucleus projecting to the principal olivary nucleus and the posterior and anterior interpositus nuc ... | 1976 | 63472 |
| horseradish peroxidase: an improvement in intracellular staining of single, electrophysiologically characterized neurons. | 1976 | 63386 | |
| the climbing fibers of the cerebellar cortex, their origin and pathways in cat. | the sources and pathways of the climbing fibers to the cerebellar posterior vermis were studied with comibined electrophysiological and anatomical methods in cats. recording from identified cerebellar purkinje cells, monosynaptic climbing fiber (cf) responses have been obtained both for stimulation of the inferior olive (io) and various parts of the brain stem (bs). cf responses were found to of three types, io only, bs only or both io and bs. however the responses to bs stimulation were very fe ... | 1976 | 63384 |
| efferent connections of the septal area in the rat: an analysis utilizing retrograde and anterograde transport methods. | experiments were performed by either retrograde (horseradish peroxidase histochemistry) or anterograde ([3h]leucine radioautography) transport methods to determine the efferent connections of the septum in the rat. it was observed that the dorsal septum projects to the lateral preoptic area, lateral hypothalamus, periventricular hypothalamus and midline thalamus. fibers from the ventral half of the septum project topographically to the hippocampal formation, thalamus, hypothalamus and midbrain. ... | 1977 | 63306 |
| centrifugal fibers to the eye in a nonavian vertebrate: source revealed by horseradish peroxidase studies. | a source of efferent fibers to the eye of snakes of the genus thamnophis has been identified by the use of the retrograde transport of horseradish peroxidase. cell bodies of the contralateral nucleus of the ventral supraoptic decussation accumulate horseradish peroxidase after intraocular but not intraorbital injections. intraocular injections also result in anterograde transport of horseradish peroxidase to retinofugal axon terminals. intraorbital injections result in accumulation of horseradis ... | 1976 | 63147 |
| the use of glutaraldehyde-conjugated horseradish peroxidase-bovine serum albumin in the visualization of concanavalin a binding to tissue sections of human colonic tumor. | a method is reported for the preparation of glutaraldehyde cross-linked horseradish peroxidase conjugates for the visualization of concanavalin a binding to frozen sections. the binding of concanavalin a to tissue sections of human colonic tumor is used to compare staining by horseradish peroxidase conjugates and monomers. without decreasing specificity, the use of progressively larger molecular weight conjugates provides progressively greater intensity of staining of the epithelial components o ... | 1976 | 62892 |
| olfactory relationships of the telencephalon and diencephalon in the rabbit. iii. the ipsilateral centrifugal fibers to the olfactory bulbar and retrobulbar formations. | the axoplasmic retrograde transport of horseradish peroxidase (hrp) from axon terminals to their parent cell bodies and histochemical fluorescence microscopy have been used to study the ipsilateral centrifugal fibers to the olfactory bulbs and anterior olfactory nucleus in the rabbit. focal injections of peroxidase were placed unilaterally into the main or accessory olfactory bulb or into the anterior olfactory nucleus. in animals with injected hrp confined within the main bulb, perikarya retrog ... | 1976 | 62770 |
| the origin of the pretecto-olivary tract. a study using the horseradish peroxidase method. | 1976 | 62601 | |
| anatomical analysis of ventrolateral thalamic input to primate motor cortex. | 1. the origin and topographical organization of input to the arm area of the primate motor cortex from the ventrolateral thalamus were examined using the method of retrograde transport of horseradish peroxidase (hrp). 2. a thin, continuous slab of labeled neurons was found in the ventrolateral thalamus followingmultiple injections of hrp into the arm area of the motor cortex. the slab of labeled neurons was flanked, medially and laterally, by groups of unlabeled neurons. 3. the origin of ventrol ... | 1976 | 62039 |
| origins of axons in the cat's acoustic striae determined by injection of horseradish peroxidase into severed tracts. | origins and terminations of fibers of the dorsal and intermediate acoustic striae were studied by surgically severing these tracts and injecting hrp into the incision. this procedure results in filling the severed axons with hrp. filled axons were traced to cell groups of origin and to some terminations of the acoustic striae. hrp-labeled terminals were found in the cochlear nuclei as well as in periolivary cell groups. filling of cells with hrp ranged from being complete, resulting in golgi-lik ... | 1976 | 61976 |
| nigrothalamic projections in the rat as demonstrated by orthograde and retrograde tracing echniques. | the nigrothalamic projection of the rat was investigated by means of horseradish peroxidase (hrp) histochemistry and by the autoradiographic tracing technique. the use of these techniques has permitted an extension of previous descriptions by identifying a neuron population in the substantia nigra, pars reticulata (snr) as the nigrothalamic cells of origin. these calls (20-25 mu) were multipolar and were distributed throughout the snr. there was a tendency however for more labelled neurons to be ... | 1976 | 61793 |
| direct projections from the mesodiencephalic midline areas to the pericruciate cortex in the cat: an experimental study with the horseradish peroxidase method. | 1976 | 61792 | |
| a horseradish peroxidase study of the cortico-olivary projection in the cat. | 1976 | 61790 | |
| afferents to the rat substantia nigra studied with horseradish peroxidase, with special reference to fibres from the subthalamic nucleus. | 1976 | 61785 | |
| retrograde transport of horseradish peroxidase in transected axons. 3. entry into injured axons and subsequent localization in perikaryon. | horseradish peroxidase (hrp) applied to crushed mouse sciatic nerves diffused through the damaged perineurium into the endoneurium. in the injured area, hrp passed into damaged myelinated and unmyelinated axons forming columns of reaction product, which extended for several millimeters proximally to the lesion. ultrastructurally, hrp adhered to the inner surface of the axoplasm and to the surfaces of neurotubules and neurofilaments in such columns. at more proximal levels axons contained hrp in ... | 1976 | 61782 |
| movement of horseradish peroxidase in the anterior parts of the eye [proceedings]. | 1975 | 61704 | |
| comparative immunoenzymatic localization of prolactin and growth hormone in human and rat pituitaries. | twelve human and twelve rat pituitaries were stained by an immunohistochemical method using a rabbit anti-ovine prolactin serum, a rabbit anti-human growth hormone serum and a sheep anti-rabbit immunoglobulin serum conjugated with horseradish peroxidase. on the same pituitary section, growth hormone cells were stained brown by using 3-3'-diaminobenzidine as peroxidase substrate, and prolactin cells were stained purplish blue by using 4-chloro-1-naphtol. growth hormone cells outnumbered prolactin ... | 1976 | 61240 |
| the olivocerebellar projection in the cat studied with the method of retrograde axonal transport of horseradish peroxidase. iii. the projection to the vermal visual area. | horseradish peroxidase (hrp) was injected separately in one of the cerebellar lobules vi, viia, viib, viiia or viiib (together corresponding to the vermal visual area) in 17 cats. after 1-3 days the distribution of labeled cells in the inferior olive was mapped. in spite of some overlapping it is clear that the various lobules of the vermal visual area receive fibers from separate parts of a horseshoeshaped region in the caudal half of the contralateral medial accessory olive (fig. 5c). the proj ... | 1976 | 61211 |
| immune complex receptors on cell surface. i. ultrastructural demonstration of macrophages. | a method is described for ultrastructural localization of immune complex receptors on the surface of viable peritoneal exudate cells. the technique entails incubation with a soluble complex of horseradish peroxidase (hrp) and specific antibody to hrp at 4 degrees c followed by exposure to diaminobenzidine and processing for electron microscopy. the bound immune complexes were evident as focal deposits of hrp reaction product, adhering closely to the external surface of macrophages with an uninte ... | 1976 | 60441 |
| concanavalin a-horseradish peroxidase bridge staining of alpha-1 glycoproteins separated by isoelectric focusing on polyacrylamide gel. | the coomassie blue protein stain and the periodic acid-schiff stain for glycoproteins are compared to a new method of staining glycoproteins resolved electrophoretically. the method utilizes a concanavalin a-horseradish peroxidase sequence to visualize selectively glycoproteins with terminal or internal mannose or terminal n-acetylglucosamine. the method applied to characterization of m and z allele products of alpha-l-antitrypsins separated by isoelectric focusing of polyacrylamide gels slabs h ... | 1976 | 60439 |
| differences between alpha and gamma motoneurons labeled with horseradish peroxidase by retrograde transport. | 1976 | 60166 | |
| anti-streptococcal antibodies reacting with brain tissue. ii. utrastructural studies. | the reaction of rabbit anti-streptococcus pyogenes type 24 with mouse brain has been studied at the ultrastructural level using horseradish peroxidase labelled antiglobulin. it is shown that these antibodies, and by inference those found in sydenham's chorea, react with the fibrous astrocyte. the cross-reacting antigen is the glial filament or a closely associated cytoplasmic constituent. | 1976 | 60120 |
| a guide to the synaptic analysis of the neuropil. | a morphological analysis of the organization of the gray matter in the central nervous system depends on the discovery of consistent repetitive patterns. without these, the gray matter remains a chaotic jungle. an hypothesis derived from the study of a few simple regions has been developed to serve as a guide in finding these patterns. it states that all nerve fibers and terminals arising from a particular group of nerve cells, or, more precisely, a particular nerve cell type, display similar ax ... | 1976 | 59655 |
| the development of the isthmo-optic tract in the chick, with special reference to the occurrence and correction of developmental errors in the location and connections of isthmo-optic neurons. | the development of the centrifugal projection to the chick retina in the isthmo-optic tract (iot) has been studied by the retrograde transport of the enzyme marker horseradish peroxidase (hrp) injected into the eye at various times during the incubation period. some neurons in the isthmo-optic nucleus (ion--the cells of origin of the iot) can first be labeled from the eye following injections on the tenth day of incubation; after injections on the twelfth day or later, about 95% of the neurons c ... | 1976 | 58875 |
| movement of horseradish peroxidase in rabbit submandibular glands after ductal injection. | horseradish peroxidase (hrp) has been used as a tracer to study movements of solutions injected retrogradely via the duct of submandibular glands in rabbits. 0.1 ml of solution was injected either manually or by a constant hydrostatic pressure, and the subsequent distribution of hrp in the gland and duct at different times after injection has been examined histochemically at light and electron microscopical levels. shortly after the injections, strong interstitial staining for peroxidase resulte ... | 1976 | 57956 |
| entry of peroxidase into neurons of the central and peripheral nervous systems from extracerebral and cerebral blood. | autonomic preganglionic, sensory, and lower motoneuron perikarya within the central nervous system, as well as cell bodies with axons projecting to the circumventricular organs, are retrogradely labeled with horseradish peroxidase (hrp) delivered to their axon terminals by cerebral and extracerebral blood. subsequent to vascular injection of hrp into mice, blood-borne peroxidase passes across permeable vessels in muscle, ganglia, and in all circumventricular organs except for the subcommissural ... | 1976 | 57126 |
| a note on the method of retrograde transport of horseradish peroxidase as a tool in studies of afferent cerebellar connections, particularly those from the inferior olive; with comments on the orthograde transport in purkinje cell axons. | 1. injections of horseradish peroxidase (hrp) suspension were made in the cerebellar cortex of cats (most often the paramedian lobule). the staining of the cerebellar cortex and the ensuing labeling of neurons in the inferior olive were studied in experiments with variations of concentration of hrp, amounts of fluid injected, survival time and age of the animals. light microscopical studies were supplemented with electron microscopical observations. the folded cerebellar cortex offers particular ... | 1976 | 57065 |
| localization of rabbit gamma globulins in the mouse visceral yolk sac placenta. | the yolk sac placenta has been implicated previously in transmission of passive immunity to the fetus. this work uses an immunohistochemical technique devised by sternberger et al. ('69) to study this problem. rabbit serum containing gamma globulins was injected into the uterine lumen of the white swiss mouse during the last third of pregnancy. two or four hours later yolk sacs were removed and fixed in 2% paraformaldehyde or freeze-dried and fixed with paraformaldehyde vapors. finely chopped ti ... | 1976 | 56909 |
| ultrastructural differences between the preterminal nerve fibres and their endings in the mechanoreceptors, with special reference to their degeneration and mode of uptake of horseradish peroxidase. | 1976 | 56762 | |
| projection of thalamic neurons to cat primary vestibular cortical fields studied by means of retrograde axonal transport of horseradish peroxidase. | the two vestibular cortical projection areas in the anterior suprasylvian sulcus and post-cruciate dimple regions were defined by evoked potential technique in anaesthetized cats. the thalamic location of neurons with axon terminals in these fields was determined using the method of retrograde axonal transport of horseradish peroxidase. the ascending vestibular pathway appeared to be separated also at the thalamic level, where cells in the ventroposterolateral nucleus were found to project to th ... | 1976 | 56281 |
| critical factors involved in the demonstration of horseradish peroxidase retrograde transport. | 1976 | 56209 | |
| immunochemical localization of in vivo bound immunoglobulins in pemphigus vulgaris epidermis. employment of a peroxidase-antiperoxidase multistep technique for light and electron microscopy. | a multi-step immunocytochemical method utilizing horseradish peroxidase (hrp) as an immunological marker was employed to demonstrate, at the ultrastructural level, igg antiepithelial autoantibodies bound in vivo to pemphigus epidermis, and circulating igg antibodies, using monkey oesophagus as substrate. to demonstrate igg the following antisera were employed in sequential steps: goat antihuman igg; rabbit antigoat igg; goat-anti-hrp-serum. after incubating with the final antigen, hrp, the latte ... | 1975 | 56157 |
| electron microscopic immunohistochemical identification of endothelial cells in the rabbit. | antibody to tissue factor apoprotein was adsorbed against gamma-globulin and coupled to horseradish peroxidase; this complex was applied to various rabbit tissues. the distribution of the peroxidase marker then was observed by electron microscopy. we examined fixed or frozen sections, as well as breis of aorta, vena cava, brain, heart, lung, liver, spleen, kidney, bone marrow, mesothelial gut lining, erythrocytes, and platelets. all endothelial cells that had been exposed to the antibody complex ... | 1976 | 55315 |
| light and electron microscopic observations on the anterograde transport of horseradish peroxidase in the optic pathway in the mouse and rat. | 1976 | 55292 | |
| factors affecting retrograde axonal transport of horseradish peroxidase in the visual system. | 1976 | 55291 | |
| tracing axons and axon collaterals of spinal neurons using intracellular injection of horseradish peroxidase. | intracellular injection and subsequent histochemical localization of horseradish peroxidase have been used to stain the soma, dendrites, axons, and axon collaterals of spinalcervical tract neurons and unidentified dorsal horn neurons in the cat. this technique may be used in combination with the intracellular injection of procion yellow to demonstrate by light microscopy connections between physiologically typed vertebrate neurons. | 1976 | 54936 |
| retrograde transpost of horseradish peroxidase in afferent and efferent neurons from masseter muscle in the rat. | 1975 | 54884 | |
| prenatal development of central optic pathways in albino rats. | the development of the central optic projections in albino rat fetuses has been studied using light and electron microscopic degeneration techniques and the horseradish peroxidase method for demonstrating axonal projections of neurons. the first optic axons to reach the region of the optic chiasm arrive at day 15. by day 16, a substantial optic chiasm is seen and the optic tract can be traced into the epithalamus, having first passed through the ventral lateral geniculate nucleus and a thin lami ... | 1976 | 54370 |
| in vitro formation of gap junction vesicles. | a method is described that uses trypsin digestion combined with collagenase-hyaluronidase which produces a population of gap junction vesicles. the hexagonal lattice of subunits ("connexons") comprising the gapjunctions appears unaltered by various structural criteria and by buoyant density measurements. the gap junction vesciles are closed by either a single or a double profile of nonjunctional "membrane," which presents a smooth, particle-free fracture face. horseradish peroxidase and cytochro ... | 1976 | 54358 |
| rôle of axonal transport in maintaining central synaptic connections. | synaptic boutons on the somata of rat hypoglossal neurones have been stained with zinc iodide-osmium, and counted using light microscopy. two weeks after silastic cuffs containing colchicine or vinblastine were applied to the hypoglossal nerve, the number of boutons on parent nerve cell bodies was significantly lower than controls. these drugs inhibited retrograde axonal transport of horseradish peroxidase, but did not affect neuromuscular conduction or the number of axons in the nerve trunk. cu ... | 1975 | 54262 |
| electron microscopic demonstration of immunoglobulin deposition in rheumatoid cartilage. | horseradish peroxidase (hrpo) conjugated with goat antihuman igg, goat antihuman igm, and aggregated human igg has been used as a enzymatic marker to stain igg, igm, and rheumatoid factor in rheumatoid cartilage. when hrpo-anti igg and hrpo-anti igm were used, immunoglobulin deposits were not observed in nonrheumatoid cartilage. however 7 of 8 rheumatoid cartilage specimens stained with hrpo-anti igg showed electron-dense deposits. three rheumatoid specimens stained with hrpo-anti igm showed sim ... | 1975 | 54168 |
| localization of laryngeal motor neurons in the kitten. | in a series of 12 newborn kittens, horseradish peroxidase (hrp) was used to trace retrograde axoplasmic flow in the motor neurons to laryngeal muscles. the animals were sacrificed 24 hours after injection of hrp into a specific laryngeal muscle, and the brain stems were stained for peroxidase. this clear-cut colorimetric method permitted the localization of the motor neurons in two nuclei of the ipsilateral brain stem. these are the nucleus ambiguus and the retrofacial nucleus. the primary sourc ... | 1975 | 53770 |
| retrograde transport of horseradish peroxidase in transected axons. ii. relations between rate of transfer from the site of injury to the perikaryon and onset of chromatolysis. | the sciatic nerves of 2--3 month-old mice, rats and rabbits were crushed and at the same time horseradish peroxidase (hrp) was applied around the crushed region at mid-thigh level. at varying time intervals thereafter, hrp was seen in neurons of the corresponding spinal ganglia and levels of the spinal cord. the rate of ascent of hrp appeared to be similar in the different animals and to be independent of the length of the nerve. the accumulation of hrp was more conspicuous in small than large n ... | 1975 | 53277 |
| the pinocytic rate of activated macrophages. | peritoneal macrophages from mice injected 4 days previously with brewer's thioglycollate medium have a pinocytic rate, in culture, of 190 ng horseradish peroxidase (hrp)/100 mug cell protein/h, compared to the rate of resident peritoneal cells of 53 ng hrp/100 mug cell protein/h. mice injected with endotoxin or with only certain of the components of the brewer's medium show an intermediate level of stimulation. the rate of unstimulated, endotoxin-stimulated, or thioglycollate-stimulated cells sh ... | 1975 | 53258 |
| preganglionic innervation of the adrenal gland of the rat: a study using horseradish peroxidase. | 1975 | 53160 | |
| a study of the dynamics of retrograde transport and accumulation of horseradish peroxidase in injured neurons. | the phenomenon of retrograde intraaxonal transport of extracellular markers introduced at the level of the axon terminal has been suggested as a possible mechanism of communication between the axon terminal and the neuron cell body. we tested the hypothesis that communication after axotomy might consist of a change in the rate of uptake or of transport of material by injured neurons. small lesions were made with a needle in one retinal quadrant of chicks and immediately afterwards horseradish pe ... | 1975 | 53088 |
| localization of alpha-d-glucosyl and alpha-d-mannosyl groups of mucosubstances with concanavalin a and horseradish peroxidase. | concanavalin a is a lectin which is known to bind specifically to alpha-d-glucosyl and alpha-d-mannosyl groups in the mucosubstances of mammalian tissues. the lectin molecule is bivalent; after its attachment to mucosubstances present in a histological specimen it can also bind horseradish peroxidase, a mannose-containing glycoprotein. the attached peroxidase may then be visualized by virtue of its histochemically demonstrable enzymatic activity. other investigators have utilized this principle ... | 1975 | 52638 |
| a dual marking technique for microelectrode tracks and localization recording sites. | this paper describes techniques for marking both microelectrode tracks and exact recording loci using a combination of fast green dye and horseradish peroxidase (hrp). the procedure involves coating the exterior of hrp filled microelectrodes with fast green dye in order to identify electrode tracks, and ejecting hrp from the electrode to mark recording loci. rapid, multiple marks can be made with this technique without harming the recording capabilities of the micropipette. | 1975 | 51724 |