Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| the second cholera toxin, zot, and its plasmid-encoded and phage-encoded homologues constitute a group of putative atpases with an altered purine ntp-binding motif. | it is shown that the second cholera toxin, zot, orf3 product of pseudomonas plasmid pkb740, and orf424 product of bacteriophage pf1 are a group of closely related proteins containing a modified version of the purine ntp-binding motif, with a drastic substitution of tyrosine for a conserved glycine. they are distantly but reliably related to the product of gene i of filamentous bacteriophages which is a putative atpase containing the classical ntp-binding motif and is involved in bacteriophage as ... | 1992 | 1426234 |
| the protease from vibrio cholerae nicks arginine at position 192 from the n-terminus of the heat-labile enterotoxin a subunit from enterotoxigenic escherichia coli. | it was examined where a protease purified from vibrio cholerae might nick the heat-labile enterotoxin (lt) a subunit from enterotoxigenic escherichia coli. lt was digested by the protease and contained a fragment which had the same mobility on sds-page as that of the a1 fragment of lt digested by trypsin. the biological activity of lt by this protease was also identical to that of lt by trypsin. the amino acid sequence of the n-terminus of the a2-like fragment was thr-ser-thr-gly, which correspo ... | 1992 | 1426177 |
| improved detection of disease progression in ocular cicatricial pemphigoid. | cicatricial pemphigoid is an autoimmune systemic disease characterized by chronic conjunctival cicatrization. the natural history is one of variable degrees of progressive scarring of the ocular surface and visual loss. previous reports have shown systemic chemotherapy to be efficacious in halting cicatrization in progressive cases. this observation makes it of critical importance to be able to detect progression of the disease as early as possible so as to allow for earlier institution of treat ... | 2006 | 1424674 |
| cellulose acetate electrophoretic determination of bone alkaline phosphatase activity in healthy subjects and diabetic patients with and without osteopenia. | we adapted the electrophoretic method of bone alkaline phosphatase (alp) determination using neuraminidase from vibrio cholerae to separate bone and liver alp on cellulose acetate membrane. treatment of separator plus serum (1:8, neuraminidase 111 u/l in final) for 10 min at room temperature (25 +/- 1 degree c) and subsequent electrophoresis made it possible to quantify bone alp activity simply and rapidly. the precision of the data was at the level of cv of 1.6% (within-day) and 4.7% (day-to-da ... | 1992 | 1424153 |
| an evaluation of the sat in the diagnosis of typhoid. | salmonella typhi o and h antibody titres were determined by the standard agglutination test (sat) in 85 patients with bacteriologically proven typhoid, 102 patients with non-typhoidal febrile illnesses (control group 1), and 170 healthy subjects (control group 2). an o antibody titre of 1/120 occurred in 93% of patients with typhoid fever (7% false negatives), and in 17% and 12% control groups 1 and 2 respectively. an h antibody titre of 1/120 was found only in 64% of patients with typhoid (fals ... | 1992 | 1423751 |
| several medical schools have begun to tackle sexual harassment issue. | 1974 | 1423097 | |
| re: current status of radiosurgery for arteriovenous malformations. | 1973 | 1423050 | |
| laparoscopic vagotomy: a new tool in the management of duodenal ulcer disease. | 2006 | 1422777 | |
| results of the first year of breast cancer screening in a district hospital. | the humberside breast screening service completed 1 year of screening by october 1990; 16,534 women were invited of whom 12,832 (77.6 per cent) attended. a group of 644 women (5.0 per cent) were called for further assessment and of these 134 underwent 135 primary surgical procedures. after assessment 25 were shown before operation to have cancer. one hundred and nine women had suspicious lesions and proceeded to surgical biopsy; 50 lesions proved malignant (benign: malignant biopsy ratio 1.2:1). ... | 2006 | 1422757 |
| intracoronary papaverine and complete atrioventricular block. | 2002 | 1422403 | |
| acquired immunity to larvae of amblyomma marmoreum and a.hebraeum by tortoises, guinea-pigs and guinea-fowl. | a study of acquired resistance in guinea-pigs, guinea-fowl and tortoises to larvae of the ticks amblyomma hebraeum koch and a.marmoreum koch (acari: ixodidae) showed that repeated infestations of the laboratory host resulted in a 29.3-49.4% reduction respectively in weight of engorged larvae. no resistance was induced in guinea-fowl, the natural host for larvae of both species. a small decline in the mean weight of engorged larvae was observed for both species of ticks after three successive inf ... | 1992 | 1421505 |
| cell cycle kinetics in malignant lymphoma studied with in vivo iododeoxyuridine administration, nuclear ki-67 staining, and flow cytometry. | cell cycle kinetics of malignant lymphoma were investigated using in vivo labeling with iododeoxyuridine (idurd) and subsequent flow cytometry (fcm) of idurd/dna and ki-67/dna. this approach provides an extensive cell kinetic profile from only one single tumor biopsy, including data upon the percentage of s-phase cells, the idurd labeling index (li), ki-67-derived growth fraction, duration of the s-phase, duration of the g1-phase, potential doubling time, cell production rate, and total cell cyc ... | 2006 | 1421404 |
| a rapid in situ respiration test for measuring aerobic biodegradation rates of hydrocarbons in soil. | an in situ test method to measure the aerobic biodegradation rates of hydrocarbons in contaminated soil is presented. the test method provides an initial assessment of bioventing as a remediation technology for hydrocarbon-contaminated soil. the in situ respiration test consists of ventilating the contaminated soil of the unsaturated zone with air and periodically monitoring the depletion of oxygen (o2) and production of carbon dioxide (co2) over time after the air is turned off. the test is sim ... | 2006 | 1418936 |
| degree of associative relatedness and sentence processing by adolescents with and without mental retardation. | previous research has suggested that individuals with mental retardation are less likely than individuals without mental retardation to access and incorporate information about the relations between words of sentences in the representations of those sentences in memory (e.g., merrill & bilsky, 1990; merrill & jackson, in press). a cued recall study and a semantic verification study were conducted to determine whether the magnitude of this group difference could be made smaller by increasing the ... | 2006 | 1418932 |
| genetic epidemiology of insulin-dependent diabetes mellitus: international comparisons using molecular genetics. | international comparative molecular studies of insulin-dependent diabetes mellitus can be viewed from an immunogenetical and an epidemiological perspective. each approach independently contributes to our knowledge concerning the etiology and prevention of the disease. however, the combination of these perspectives, representing an interface between molecular biology and epidemiology, will achieve more in terms of scientific advancement and public health than either perspective alone. an excellen ... | 2006 | 1418925 |
| regulation of erythropoiesis in the newborn: a complex system. | 2006 | 1418910 | |
| the utility of magnetic resonance imaging in the diagnosis of intranasal meningoencephaloceles. | we present three patients in whom the diagnosis of intranasal meningoencephalocele was made by magnetic resonance imaging. the initial clinical evaluation and computed tomographic examinations of these patients failed to distinguish between chronic inflammation and intranasal meningoencephalocele. although both computed tomography and magnetic resonance imaging are used to distinguish between normal, inflammatory, and neoplastic tissue in the nasal cavity and paranasal sinuses, limitations do ex ... | 2006 | 1418906 |
| an n-hydroxylated derivative of cyanamide that inhibits yeast aldehyde dehydrogenase. | a stable, n,o-dibenzoyl derivative (dbhc) of n-hydroxycyanamide, the latter the postulated bioactivation product of the alcohol deterrent agent, cyanamide, has been synthesized. dbhc was an effective inhibitor of yeast aldehyde dehydrogenase (aidh) in vitro and inhibited this enzyme in a concentration-dependent manner with an ic50 of 25 microm. hydrolysis of the benzoate moiety of dbhc with dilute naoh gave rise to the formation of nitroxyl (hn = o), detected by gas chromatography as nitrous oxi ... | 1992 | 1418657 |
| [professional profile nursing. who will do something with it?]. | 2006 | 1418639 | |
| elisa for seroepidemiological study of exposure to vibrio cholerae of population in krabi province, thailand. | seroepidemiological study of vibrio cholerae exposure was carried out in krabi province during january 1989 to december 1990 using indirect elisa to determine serum antibodies to lipopolysaccharides (lps) of v. cholerae. among 363 serum samples obtained from cord blood and venous blood of healthy persons, aged from 6 months to over 50 years, 65% and 64% were found positive for specific igg and igm against lps of v. cholerae, respectively. the seroprevalence of v. cholerae infection increased wit ... | 1992 | 1418185 |
| miliary tuberculosis involving the brain: mr findings. | 2004 | 1414778 | |
| [the fatty acid composition of the total lipids in vibrio cholerae]. | the fatty acid composition of total cell lipids of v. albensis typing strains, serovars 01-012, isolated from patients and environmental objects, as well as v. eltor and nag vibrios, has been studied. fatty acids contain 14-18 carbon atoms and are mainly represented by unsaturated monoacids. palmitoleic and oleic acids constitute the greater part of unsaturated acids (their total content is 55.2-71.0%). the level of saturated fatty acids in considerably lower (27.0-43.1%), and these acids are ma ... | 1992 | 1414098 |
| [electrocardiographic diagnosis of acute graft rejection after heart transplantation]. | in order to evaluate the diagnostic value of standard-ecg (st-ecg) and precordial mapping-ecg with 63 unipolar leads (pm-ecg) for detection of acute cardiac allograft rejection, 15 patients (12 male, 3 female; age range 24-64 years) were studied. st-ecg and pm-ecg were recorded along with 94 endomyocardial biopsies. twenty-four acute rejections were detected histologically. using the st-ecg, a reduction of the qrs-amplitude-sum (lead i, ii, iii, v1 and v6) greater than or equal to 5% in comparis ... | 2006 | 1413949 |
| radiotherapeutic management in carcinoma of the uterine cervix: gog experience. | 2006 | 1413899 | |
| single and multigland disease in primary hyperparathyroidism: clinical follow-up, histopathology, and flow cytometric dna analysis. | two-hundred seventy-four patients with primary hyperparathyroidism had selective removal of enlarged parathyroid glands. biopsies were taken from all parathyroid glands. normal-size glands were not resected irrespective of their histological appearance. after a mean follow-up of 13.5 years the rates of persistent and recurrent hyperparathyroidism were, respectively, 3.6% and 0.7%. transient and permanent hypoparathyroidism occurred in 24% and 2.5% of the patients. the microscopic appearance of e ... | 2006 | 1413843 |
| differentiated thyroid carcinoma in children and adolescents. | from 1936 to 1990, 89 children and adolescents (72 girls and 17 boys) were treated for differentiated thyroid carcinoma at the university of michigan medical center; 58 of these patients were first reported in 1971. thirty (34%) patients had previously received external irradiation to the head and neck, although only 1 (3%) patient of the last 33 patients seen had this history. patients first presenting from 1971 to 1990 had less advanced disease than those seen earlier. also, during this time p ... | 2006 | 1413825 |
| [long-term results in children with classical galactosemia]. | galactosemia due to uridyl-transferase deficiency was detected in 47 patients between 1/1/1967 and 31/12/1988. the condition took a fulminant course in 31 patients and 10 infants in this group died between the 6th and the 18th day of life. the determination of uridyl-transferase in 36 patients confirmed the diagnosis. exchange blood transfusion was undertaken in 25 infants because of hyperbilirubinemia, septicemia or neurological symptoms. 5 of the 31 children had a cataract. in most cases the l ... | 2006 | 1413810 |
| [the enzymatic activity of ammonia metabolism in the liver during the parenteral nitrogen feeding of animals with experimental liver failure]. | it was established that liver insufficiency the activity of hepatic glutamate dehydrogenase is markedly reduced that is, apparently, the main cause of hyperammonemia that accompanies hepatic encephalopathy. here occurs a significant reduction of the processes of ammonia detoxication with formation of glutamine in the liver as evidenced by changes of the activity of glutamine synthetase. administration parenterally of a preparation of nitrous feeding increases essentially ammonia detoxicating fun ... | 1992 | 1413686 |
| development of a local network for liver transplantation in the kinki region of japan: two-year experience of the recipient selection committee. recipient selection committee members of the kinki network for liver transplantation. | 1974 | 1412975 | |
| emr, an escherichia coli locus for multidrug resistance. | an escherichia coli chromosomal dna fragment cloned on a multicopy plasmid conferred resistance to carbonylcyanide m-chlorophenylhydrazone, nalidixic acid, and a number of other toxic compounds. the sequence of the cloned emr locus located at minute 57.5 of the chromosome revealed two open reading frames, emra and emrb. emrb encodes a highly hydrophobic 56.2-kda peptide, with 14 potential alpha-helices to span the inner membrane. the peptide is homologous to qaca, a multidrug-resistant pump from ... | 1992 | 1409590 |
| existence of a natural killer (nk) cell repertoire for (allo)antigen recognition: definition of five distinct nk-determined allospecificities in humans. | 1968 | 1409338 | |
| a simple purification method of vibrio cholerae non-o1 hemagglutinin/protease by immunoaffinity column chromatography using a monoclonal antibody. | a new simple purification method (i) for vibrio cholerae non-o1 hemagglutinin/protease (nag-ha/p) was developed. the method (i) requires only an immunoaffinity column chromatography using a monoclonal antibody against nag-ha/p. the method (i) is much simpler than previously reported purification method (ii) (honda, t. et al, infection and immunity 57: 2799-2803, 1989) which required four or more complicated chromatographic procedures. method (i) also gave an improved recovery rate (about 27%) co ... | 1992 | 1406368 |
| characterization of a vibrio cholerae virulence factor homologous to the family of tonb-dependent proteins. | irga is an iron-regulated virulence factor for infection in an animal model with classical vibrio cholerae strain 0395. we detected gene sequences hybridizing to irga at high stringency in clinical isolates in addition to 0395, including another classical strain of v. cholerae, three v. cholerae strains of the el tor biotype, three non-o1 isolates of v. cholerae, and individual isolates of vibrio parahaemolyticus, vibrio fluvialis, and vibrio alginolyticus. no hybridization to irga was seen with ... | 1992 | 1406279 |
| [development and application of polymerase chain reaction (pcr) and dna hybridization for detection of cholera enterotoxin-producing v. cholerae and other pathogens]. | 1992 | 1404923 | |
| block of voltage-gated potassium currents by anticonvulsant u-54494a in mouse neuroblastoma cells. | u-54494a [(+-)-cis-3,4-dichloro-n-methyl-n-[2-(1- pyrrolidinyl)-cyclohexyl]-benzamide], an anticonvulsant and na channel blocker, was examined for its interaction with delayed rectifier k channels in mouse neuroblastoma cells (nie-115) using whole-cell and inside-out configurations of the patch clamp techniques. u-54494a at anticonvulsant doses reversibly blocked the tetraethylammonium-sensitive outward k current in a time-dependent manner without affecting the current-voltage relationship. anal ... | 1992 | 1403785 |
| proposal of a computerized algorithm for continuous wave co2 laser on-line control during orthopaedic surgery. phase i: theoretical introduction and first in vitro trials. | new data obtained from treating polymethylmethacrylate (pmma) with a non-moving cw- 10 watt-co2 laser-beam focused at 2.5'', 5'', 7.5'' and 15.75'' are presented. . the final equations r(tc) and z(tc) for each focal length are proposed. a very interesting correlation between the focal lengths in use and the integrated values of r and z between 0 and 2 sec has been identified and discussed. this result has been used as basis to define a convenient operative protocol to follow during the planning ... | 2006 | 1402302 |
| [a case of vibrio cholerae non-o1 septicemia with liver cirrhosis]. | a case of vibrio cholerae non-o1 septicemia is described in this paper. a 45-year-old male with a three year history of liver cirrhosis, was admitted to our division with hematemesis, abdominal pain, high fever and a loss of consciousness. three days before onset of symptoms, he traveled to ishigaki island and ate a raw lobster. two days after, his temperature rose to 39.7 degrees c and the blood pressure dropped to 36/- mmhg. by endoscopic examination, an ulcer was found in the stomach, and the ... | 1992 | 1402101 |
| [in vitro antimicrobial activity of dr-3355, a new quinolone antibacterial agent, against clinical isolates of enteritis-causing bacteria]. | we determined the minimum inhibitory concentration (mic) of dr-3355, a newly developed quinolone-derivative antibacterial agent, against clinical isolates of various bacterial species from enteritis patients, and compared them with those of ofloxacin (oflx), ciprofloxacin (cpfx), nalidixic acid (na), ampicillin (abpc), kanamycin (km). mic90 of dr-3355 against 94 strains of shigella spp. and 5 strains of escherichia coli, 36 strains of salmonella spp., 22 strains of vibrio cholerae, 5 strains of ... | 1992 | 1402063 |
| evidence that inactivated oral cholera vaccines both prevent and mitigate vibrio cholerae o1 infections in a cholera-endemic area. | in a randomized, placebo-controlled field trial of b subunit-killed whole cell (bs-wc) and killed whole cell only (wc) inactivated oral cholera vaccines in rural bangladesh, active surveillance of selected neighborhoods during the first year after vaccination identified 127 vibrio cholerae o1 infections among 3285 three-dose recipients. for each vaccine, protective efficacy was greater against symptomatic (57%, p < .05 for bs-wc; 58%, p < .05 for wc) than against asymptomatic infections (46%, p ... | 1992 | 1402014 |
| development and testing of a nonradioactive dna oligonucleotide probe that is specific for vibrio cholerae cholera toxin. | an alkaline phosphatase-labeled oligonucleotide dna probe (ctap) that was specific for the cholera toxin gene (ctxa) was identified. all cholera toxin-producing strains of vibrio cholerae, regardless of serotype, hybridized with the ctap probe, while nontoxigenic strains from either environmental sources or from deletion or substitution mutations did not hybridize. unlike the whole-gene probes for either ctxa or for the heat-labile toxin or escherichia coli (elta), this 23-base sequence did not ... | 1992 | 1400994 |
| vibrio-associated gastroenteritis in the lower cross-river basin of nigeria. | a total of 120 vibrio species were isolated from 588 patients with acute diarrheal disease during an outbreak of gastrointestinal tract infections at different locations in the lower cross river basin of nigeria. vibrio cholerae o1, biotype el tor, serotype ogawa, was the prominent organism isolated from the vibrio-associated diarrheal cases. during the 3 months of study, v. cholerae non-o1 was recovered from 10 patients while vibrio parahaemolyticus was recovered from 19 patients. the significa ... | 1992 | 1400976 |
| specific detection of campylobacter jejuni and campylobacter coli by using polymerase chain reaction. | development of a routine detection assay for campylobacter jejuni and campylobacter coli in clinical specimens was undertaken by using the polymerase chain reaction (pcr). an oligonucleotide primer pair from a conserved 5' region of the flaa gene of c. coli vc167 was used to amplify a 450-bp region by pcr. the primer pair specifically detected 4 strains of c. coli and 47 strains of c. jejuni; but it did not detect strains of campylobacter fetus, campylobacter lari, campylobacter upsaliensis, cam ... | 1992 | 1400961 |
| the virulence gene activator toxt from vibrio cholerae is a member of the arac family of transcriptional activators. | virulence gene expression in vibrio cholerae is postulated to involve toxr-dependent activation of the toxt gene followed by toxt activation of virulence genes, including several of those involved in biogenesis of the toxin-coregulated pilus. toxr is a transmembrane, dna-binding protein which is a member of the ompr subclass of two-component activator systems in bacteria. data presented in this report demonstrate that toxt is similar to the arac family of transcriptional activators identified in ... | 1992 | 1400247 |
| toxr proteins with substitutions in residues conserved with ompr fail to activate transcription from the cholera toxin promoter. | the toxr protein of vibrio cholerae is an integral membrane protein that coordinately regulates the expression of virulence genes required for successful infection. toxr has been shown to bind directly to and activate transcription of the cholera toxin (ctx) promoter. within the amino-terminal cytoplasmic region of toxr, several amino acids are strictly conserved among toxr, ompr, and the other members of a family of bacterial regulatory proteins. to better understand the function of this region ... | 1992 | 1400230 |
| a 14-kilodalton inner membrane protein of vibrio cholerae biotype e1 tor confers resistance to group iv choleraphage infection to classical vibrios. | choleraphage phi 149 differentiates the two biotypes, classical and el tor, of vibrio cholerae. this phage cannot replicate in v. cholerae biotype el tor cells because the concatemeric dna intermediates produced are unstable and cannot be chased to mature phage dna. a v. cholerae biotype el tor gene coding for a 14,000-da inner membrane protein which destabilizes the concatemeric dna intermediates by hindering their binding to the cell membrane has been identified. presumably, a 22,000-da v. cho ... | 1992 | 1400172 |
| fusion proteins containing the a2 domain of cholera toxin assemble with b polypeptides of cholera toxin to form immunoreactive and functional holotoxin-like chimeras. | cholera enterotoxin (ct) is produced by vibrio cholerae and excreted into the culture medium as an extracellular protein. ct consists of one a polypeptide and five b polypeptides associated by noncovalent bonds, and ct-b interacts with ct-a primarily via the a2 domain. treatment of ct with trypsin cleaves ct-a into a1 and a2 fragments that are linked by a disulfide bond. ct-b binds to ganglioside gm1, which functions as the plasma membrane receptor for ct, and the enzymatic activity of a1 causes ... | 1992 | 1399002 |
| major outer membrane proteins of vibrio cholerae and their role in induction of protective immunity through inhibition of intestinal colonization. | vibrio cholerae o1 organisms belonging to different biotypes and serotypes were shown to express major outer membrane proteins (momps) with subunit molecular masses of 48 to 50, 40 to 43, 35 to 36, 27 to 28, and 20 kda. antisera raised against individual momps of a v. cholerae o1 strain recognized momps of corresponding molecular masses in other o1 and non-o1 strains. serological data also suggested possible differences in the cell surface exposition of these momps. however, no marked difference ... | 1992 | 1398996 |
| association of giardia lamblia with vibrio in cholera cases. | 1992 | 1398879 | |
| genetics of cholera toxin. | cholera is caused by the toxin secreted by vibrio cholerae 01. cholera toxin (ct) is a protein consisting of a and b subunits. the former contributes to intracellular toxicity whereas the b subunit is required for binding of ct to eukaryotic cell surface receptor. the structural genes encoding a and b subunits are designated as ctxa and ctxb respectively. these genes are located on the chromosome forming an operon in which ctxa precedes ctxb. the ctxab have been cloned and sequenced. classical s ... | 1992 | 1398801 |
| cyclosporin a treatment of young children with newly-diagnosed type 1 (insulin-dependent) diabetes mellitus. london diabetes study group. | several studies have demonstrated the efficacy of cyclosporin a in modifying the initial course of type 1 (insulin-dependent) diabetes mellitus in older children and adults but none have reported the effects in very young children. we treated 14 newly-diagnosed type 1 diabetic patients aged 22 months to 95 months with cyclosporin a. mean insulin dose at entry was 0.7 +/- 0.07 iu.kg-1.day-1. initial cyclosporin a dose was 10 mg.kg-1.day-1. insulin dose reached a nadir of 0.13 iu.kg-1.day-1 by 180 ... | 1992 | 1397785 |
| foot infections in diabetes are rarely due to a single microorganism. | the purpose of this study was to investigate the frequency with which multiple organisms can be isolated from severe foot infections in diabetic patients with carefully planned techniques of collection and processing of specimens. one hundred and seventy-seven organisms were isolated from 52 patient admissions (mean 3.4 per infection) at a district hospital. this was compared to a mean of 2.1 organisms per lesion at a local university hospital and 2.3 at a smaller community hospital. staphylococ ... | 1992 | 1395469 |
| parity, ethnic group and the prevalence of type 2 diabetes: the coventry diabetes study. | the prevalence of type 2 (non-insulin-dependent diabetes) in relation to parity was compared among south asian (asian) and european women during a cross-sectional house-to-house screening programme for diabetes in foleshill, coventry, uk. the parity of female residents was ascertained in 8 of the 12 areas visited. these areas contained 2096 european (68 with diabetes diagnosed) and 1148 asian women (95 with diabetes diagnosed). crude prevalence of type 2 diabetes was 3.2% and 14.7% in europeans ... | 2006 | 1395461 |
| cytochemical characterization of the cuticle of caenorhabditis elegans (nematoda: rhabditoidea). | at the ultrastructural level, the caenorhabditis elegans (maupas, 1900; doughert, 1953) cuticle shows the presence of six layers: epicuticle, external cortical, internal cortical, intermediate, fibrous and basal. two techniques were used for carbohydrate localization: the periodic acid-thiosemicarbazide-silver proteinate (thiéry) and gold-labelled lectins. no labelling was found on the nematode's cuticle. with the ethanolic phosphotungstic acid technique (e-pta), that detects basic proteins, rea ... | 1992 | 1394094 |
| medial gastrocnemius flap. | 2006 | 1393257 | |
| current treatment of ulcerative colitis. | ulcerative colitis is a chronic relapsing disease which may become manifest either early or late in life. this article examines current drug treatment strategies and considers possible therapeutic options for the future. | 2006 | 1393216 |
| antimicrobial prophylaxis for fractured base of skull in children. | the details are reviewed of 50 children who were treated over a 10-year period with clinical signs of fractured base of skull. two patients died early without signs of sepsis--due to the severity of their head injuries. of the remainder, 23 received antibiotic prophylaxis and 25 did not. one patient from each of these groups developed pneumococcal meningitis, and they were successfully treated. our results correlate well with those previously published, confirming the low incidence of infective ... | 2006 | 1393186 |
| delusional reduplication following closed-head injury. | somatic delusions following brain injury are not uncommon, and have been well documented in the literature. this study documents a case of somatic delusion which was seen in a patient following a head injury secondary to a motorcycle accident. although perhaps not typical it serves to illustrate an interesting example of a somatic delusion following head trauma. on recovery from coma this patient reported the existence of a 'third arm' adjacent to the limb that had received the greatest impact i ... | 2006 | 1393179 |
| asthma and open cast mining. | 2006 | 1393082 | |
| health checks on patients 75 years and over in nottinghamshire after the new gp contract. | to investigate annual health checks for patients of 75 years and over required by the 1990 contract for general practitioners. | 2006 | 1393076 |
| reprocessing data to form qalys. | 2006 | 1392941 | |
| early parenteral penicillin in meningococcal disease. | 2006 | 1392928 | |
| cholera. | 1992 | 1392799 | |
| management of an emergency: to be prepared for the unwanted event. | every dental office needs an adequate emergency treatment structure. while theoretical and practical pregraduate education in emergency medicine is included (in differing degrees) in the curricula of dental schools throughout the world, postgraduate training is generally undertaken on a voluntary basis. repeated training in emergency techniques is encouraged for the dentist and dental office staff, as the trained and coordinated action of the whole team in the dental office is necessary when an ... | 2006 | 1392691 |
| interview with merri morris, m. d.. interview by ina may gaskin. | 2006 | 1392637 | |
| [diarrhea in a woman from peru]. | 1992 | 1391004 | |
| value of transesophageal color doppler echocardiography for detection of different types of atrial septal defect in adults. | in 121 adults, the value of transthoracic and transesophageal color doppler echocardiography for detection of different types of atrial septal defect (asd) or of partial anomalous pulmonary venous return was analyzed. the 121 patients had a total of 129 defects with left-to-right atrial shunting (including eight patients with two types of defects). all of six cases with primum-type asd were diagnosed correctly by both echocardiographic methods. ninety-seven patients showed a secundum-type asd du ... | 1997 | 1389216 |
| cholera as a model for research on mucosal immunity and development of oral vaccines. | during the past year, the extensive investigational use of a recently developed oral vaccine against cholera has led to significant advances in our understanding of both immunity to cholera and related diarrhoeal diseases, and the mucosal immune response in general after oral immunization. the oral cholera vaccine has been shown to protect, through its cholera toxin b subunit component, against travellers' diarrhoea caused by enterotoxigenic escherichia coli. the elaboration of sensitive new tec ... | 1992 | 1388838 |
| suppression of the arthus reaction by y-24180, a potent and specific antagonist of platelet-activating factor. | a novel and potent antagonist of platelet-activating factor (paf), y-24180 (4-(2-chlorophenyl)-2-[2-(4-isobutylphenyl)ethyl]-6,9-dimethyl-6h- thieno[3,2-f][1,2,4]triazolo[4,3-a][1,4] diazepine) was investigated for the effects on the skin reactions induced by chemical mediators and the arthus reactions. in the rat dorsal skin, y-24180 (0.1-10 mg/kg, p.o.) inhibited increase in vascular permeability by the intradermal paf injection in a dose dependent manner and the inhibitory activity was 60 tim ... | 1992 | 1388318 |
| influence of dopaminergic and serotonergic neurons on intravenous ethanol self-administration in the rat. | rats implanted with chronic indwelling intravenous catheters and allowed access to a self-administration apparatus learned to self-inject intravenous ethanol. ethanol concentrations of 0.5, 1.0, and 2.0%, corresponding to a dose/injection of 1, 2, and 4 mg/kg, respectively, were consistently self-injected. self-injection was not acquired or maintained with ethanol doses of 0.5 or 8 mg/kg/injection. saline replacement of ethanol reservoirs led to marked increases in lever-pressing response in ani ... | 1992 | 1388276 |
| [antibiotic sensitivity of vibrio cholerae 01 isolated from environmental objects]. | the sensitivity of 252 vibrio cholerae-o1 strains isolated from environmental objects to antibiotics of various groups was assayed by the method of serial dilutions on solid media. the biological characteristics of the isolates are presented. the vibrio cholerae isolates with serological variation were the most frequent (36.6 per cent), so are the cultures detected by their sensitivity to the specific phages (87.5 per cent). it was found that changes in some biological properties of the strains ... | 1992 | 1387519 |
| [study of the epidemic significance of noncultivated forms of vibrio cholerae by the polymerase chain reaction]. | a specific method of the isolation of the cholera toxin gene by the directional amplification of dna in the polymerase chain reaction (pcr) has been developed. the product of this reaction has a molecular weight of 440 sequence pairs and is a dna fragment located on the a-subunit of v. cholerae gene vct. the sensitivity of the method permits the detection of one bacterial cell in the reaction mixture. the method is effective when v. cholerae purified dna, cell lysates and the dna of total microf ... | 1992 | 1387276 |
| [allergic drug reactions in children]. | we present a retrospective study of allergic drug reactions seen in our pediatric allergy consulting room during the last 6 years. during this time, 840 patients were examined for suspected adverse drug reactions. drug allergy was confirmed in 72 cases (8.5%). of these cases, 29 (40.2%) were considered to be ige mediated, or immediate hypersensitivity reactions. we have not found significant differences with regards to age, sex, atopic family history or atopia between patients with ige mediated ... | 2006 | 1386717 |
| body stalk anomaly: congenital absence of the umbilical cord. | on ultrasound examination, a 20-year-old pregnant woman was found to have a fetus with a large ventral abdominal wall defect diagnosed as a fetal omphalocele. the neonate was delivered by cesarean; the abdominal viscera including liver, stomach, spleen, pancreas, intestine, and uterus were contained in an extraembryonic sac directly attached to the placenta without an umbilical cord. body stalk anomaly (also known as absence of the umbilical cord syndrome) is a fatal condition resulting from mal ... | 2006 | 1386663 |
| laparoscopic co2 laser uterine nerve ablation for treatment of drug resistant primary dysmenorrhea. | twenty women with drug-resistant primary dysmenorrhea were subjected to laparoscopic co2 laser uterine nerve ablation. menstrual pain assessed by a linear analog pain score showed a reduction of 33%, decreasing from 7.5 +/- 0.5 preoperatively to 5.0 +/- 1.7 postoperatively. the procedure was free of major complications and should be considered as a second-line therapeutic option in women who have failed medical treatment using nonsteroidal antiinflammatory agents or ocs. | 2006 | 1386032 |
| the treatment of simultaneous choriocarcinoma in mother and baby. | simultaneous occurrence of choriocarcinoma in mother and child is rare. such a case is described in which a massive intrahepatic tumor in the infant led to its treatment and that of the mother who showed evidence of the same tumor. both are alive and well 1 year after treatment, the longest recorded infant survival. | 1992 | 1383486 |
| construction of the physical maps of mycoplasma hyopneumoniae and mycoplasma flocculare and the location of rrna genes on these maps. | the genomes of mycoplasma flocculare and mycoplasma hyopneumoniae, two mycoplasmas of the porcine respiratory system, were studied. based upon antigenic cross-reactivity and dna-dna hybridization, these species have given indication of a close genetic relationship. by using field-inversion gel electrophoresis and employing the restriction digest fragments obtained from the gels as the probes, physical maps of the genomes of the two species were constructed. mycoplasma hyopneumoniae is similar to ... | 1992 | 1382823 |
| the mode of action of vibrio cholerae cytolysin. the influences on both erythrocytes and planar lipid bilayers. | the interaction with erythrocytes of cholera cytolysin (cc) obtained from a non-01 vibrio cholerae strain results in the osmotic rupture of target cells upon formation by cc of the waterfilled pores in their membranes. the aggregation of several toxin monomers is required for the formation of one cc channel with a radius of 0.9-1.0 nm. the investigations using planar bilayer lipid membranes suggest that the cc-induced pore is an interprotein anion selective channel carrying a fixed positive char ... | 1992 | 1382601 |
| cholera in 1991. | 1992 | 1382534 | |
| disparate effects of calcium channel blockers on pressure dependence of renin secretion and flow in the isolated perfused rat kidney. | using the model of isolated perfused rat kidneys this study was performed to investigate whether or not voltage-operated calcium channels are essentially involved in the pressure control of renin secretion from the kidneys. at a perfusion pressure of 100 mm hg (13.3 kpa) renin secretory rates were 4.2 +/- 0.7 (ng angiotensin i h-1) min-1 g-1. stepwise reduction of renal perfusion pressure to 80, 60, and 40 mm hg (10.6, 8.0, 5.3 kpa) resulted in an increase of renin release yielding a 30-fold sti ... | 1992 | 1382264 |
| serological cross-reaction between yersinia enterocolitica o9 and non-o1 vibrio cholerae bio-serogroup hakata and antigenic analysis of their relationship by their lipopolysaccharides. | cross-agglutination and cross-agglutinin absorption experiments were carried out on non-o1 vibrio cholerae bio-serogroup hakata (hakata) and yersinia enterocolitica o9 (o9). it was shown that the o-antigen of hakata was closely related to that of o9 in an a, b-a, c type of relationship. the antigenic relationship between the o-antigens of the two bacteria was analyzed by passive hemolysis (ph) and passive hemolysis inhibition (phi) tests by using their lipopolysaccharides (lps) as antigen for se ... | 1992 | 1381806 |
| the adjuvant effect of vibrio cholerae and escherichia coli heat-labile enterotoxins is linked to their adp-ribosyltransferase activity. | this study addressed the question of whether the mucosal adjuvant property of cholera toxin (ct) and the structurally closely related escherichia coli heat-labile toxin (lt) requires the enterotoxic and adenylate cyclase/camp activating property of these molecules. therefore, we investigated the cytotoxic and adjuvant abilities of the enterotoxins and compared the results with those obtained with the non-toxic ct and lt derivatives; recombinant ctb (rctb) and a mutated lt (mlt), which had a sing ... | 1992 | 1381311 |
| the vascular endothelial growth factor proteins: identification of biologically relevant regions by neutralizing monoclonal antibodies. | angiogenesis plays critical roles in organ development during embryonic and fetal life, wound healing and in a variety of pathological conditions. vascular endothelial growth factor (vegf) is a secreted growth factor specific for vascular endothelial cells which induces angiogenesis in vivo. to gain a better understanding of the physiological role of vegf, we have generated and characterized four murine monoclonal antibodies (mabs) using the 165 amino acid species of recombinant human vegf as im ... | 1992 | 1380254 |
| 'in vitro' action of histamine and cimetidine on amylase secretion in the rat pancreas. | we report here the effect of histamine on amylase secretion in isolated lobules from the rat pancreas. different concentrations of histamine increased amylase secretion, while the stimulatory effect was reduced by cimetidine although not by chlorpheniramine. these findings suggest that pancreatic lobules have h2 receptors. on the other hand, bethanechol induced a stimulatory effect on pancreatic lobules that was inhibited by cimetidine. vibrio cholerae toxin stimulated amylase secretion, but thi ... | 1992 | 1379840 |
| histochemical analysis of sialomucin in paget cells of mammary and extramammary paget's disease. | the cytoplasmic sialomucin in paget cells of both mammary and extramammary paget's diseases was examined, using a new method proposed by volz et al. (1987a, b). the staining methods used involved an electrolyte-alcian blue (ph = 5.8) and periodic acid schiff. oxidation was performed with 0.4 mmol/l periodate in 1 mol/l hcl at 4 degrees c or 50 mmol/l periodate in distilled water at room temperature for 1 h. methylation, saponification, borohydride reduction, and digestion with diastase, neuramin ... | 1992 | 1379408 |
| [development of oral vaccines based on recombinant proteins derived from cholera toxin]. | in this paper a new approach to create antigens through genetic engineering is discussed. in this particular case the subunits of v. cholerae toxin are used as heterologous epitope carries. in this paper the manipulation of a and b subunits is described. this manipulation allows both the insertion of epitopes to the b subunit and the use of subunit a in the construction of recombinant antigens similar to the ones derived from subunit b. | 1992 | 1377408 |
| inhibition of adhesion of s-fimbriated escherichia coli to buccal epithelial cells by human milk fat globule membrane components: a novel aspect of the protective function of mucins in the nonimmunoglobulin fraction. | we investigated the presence of factors in human milk that inhibit invasion of pathogenic bacteria. the effect of human milk fat globule membrane (hmfgm) components on adhesion of cloned s-fimbriated escherichia coli to human buccal epithelial cells was analyzed. s fimbriae are a common feature of e. coli strains causing sepsis and meningitis in newborns and are bound to epithelia via sialyl-(alpha-2-3)galactoside structures. human milk fat globules (hmfg) could be agglutinated by the above-ment ... | 1992 | 1377184 |
| differential sensitivity of cd34 epitopes to cleavage by pasteurella haemolytica glycoprotease: implications for purification of cd34-positive progenitor cells. | our previous studies have shown that a unique glycoprotease from pasteurella haemolytica specifically cleaves only proteins containing sialylated o-linked glycans. the hematopoietic progenitor cell antigen, cd34, which is heavily glycosylated with both n- and o-linked glycans, is readily cleaved by this protease. in this study, we demonstrate that the epitopes detected by five of the seven cd34 monoclonal antibodies are removed by the glycoprotease. the differential sensitivity of the cd34 epito ... | 1992 | 1375160 |
| influenza viruses differ in recognition of 4-o-acetyl substitution of sialic acid receptor determinant. | equine alpha 2-macroglobulin (em), known to contain both neu5ac and neu4,5ac2 sialic acid residues, was treated with vibrio cholerae sialidase for the selective removal of neu5ac and was compared with the untreated em for its binding by a panel of influenza viruses. type a h3n2 virus strains having leu in position 226 of their hemagglutinin (ha) changed the affinity for sialidase-treated em only slightly, if at all, indicative of their ability to bind the 4-o-ac-substituted neu5ac receptor deter ... | 1992 | 1374985 |
| serotype conversion in vibrio cholerae o1. | vibrio cholerae o1 exists as two major serotypes, inaba and ogawa, which are associated with the o antigen of the lipopolysaccharide and are capable of unequal reciprocal interconversion. the 20-kilobase rfb regions encoding o-antigen biosynthesis in strains 569b (inaba) and o17 (ogawa) have been cloned in escherichia coli k-12 and the nucleotide sequences have been determined. besides several base substitutions and a small deletion in the 569b sequence relative to o17, there is a single nucleot ... | 1992 | 1372980 |
| effects of neuraminidase on airway reactivity in the guinea pig. | we investigated the effects of neuraminidase, a viral enzyme that cleaves alpha ketosidic cell-bound sialic acids, to see if it accounts for parainfluenza and influenza virus-induced airway hyperreactivity. accordingly, vibrio cholerae neuraminidase was administered intratracheally in guinea pigs, and airway reactivity was assessed 3 h later. removal of sialic acid residues was evaluated by histologic studies. airway responsiveness was determined in anesthetized, tracheotomized, and mechanically ... | 1992 | 1372796 |
| cloning, sequencing, and transcriptional regulation of the vibrio cholerae fur gene. | many genes involved in the transport of iron by bacteria as well as in pathogenesis are regulated by the environmental concentration of iron, with increased expression under low-iron conditions. in escherichia coli, transcriptional regulation by iron depends on the fur gene. a virulence gene in vibrio cholerae (irga) is also transcriptionally regulated by iron, and the promoter of irga contains a dyad repeat homologous to fur binding sites in e. coli. southern hybridization of v. cholerae chromo ... | 1992 | 1372314 |
| biochemical and functional characterization of the leucocyte tyrosine phosphatase cd45 (cd45ro, 180 kd) from human neutrophils. in vivo upregulation of cd45ro plasma membrane expression on patients undergoing haemodialysis. | the biochemical and functional characterization, and the regulation of plasma membrane expression of the leucocyte tyrosine phosphatase cd45, have been investigated in neutrophils from healthy donors and patients undergoing haemodialysis. cd45 proteins of 180 kd and 130-150 kd were precipitated from neutrophils from both healthy subjects and haemodialysed patients. prolonged storing, as well as trypsin treatment of samples containing the 180-kd cd45 protein, generated the 130-150-kd polypeptides ... | 1992 | 1370931 |
| [initial experiences with a self-expanding metal stent in malignant ureteral compression]. | self-expanding metallic stents for the treatment of hydronephrosis were implanted in 12 ureters with malignant compression in 10 patients. in 3 ureters the stents were introduced percutaneously by a transrenal route; in 8 ureters introduction was transurethral and in one ureter by a combined route. patency rate after 6 months was 90.9%. in all cases the stent was able to withstand pressure by the tumour for the remainder of the patient's life. in one patient there was incrustation of both stents ... | 2005 | 1370762 |
| regulation of annexin i in adipogenesis: camp-independent action of methylisobutylxanthine. | the differentiation of 3t3-l1 fibroblasts to adipocytes can be accelerated by the addition of 1-methyl-3-isobutylxanthine (mix), insulin, and dexamethasone to the culture medium. during differentiation, we have demonstrated that the level of both annexin i mrna and protein decreases. the half-times for this reduction were 2 h and 10 h for annexin i mrna and protein, respectively. of the added agents in the differentiation medium, only mix caused a decline in annexin i expression in 3t3-l1 fibrob ... | 2005 | 1370745 |
| monocyte chemotactic and activating factor is a potent histamine-releasing factor for human basophils. | recombinant monocyte chemotactic-activating factor (mcaf) has been shown to induce histamine release from human basophils with a dose response between 10(-9) and 10(-6) m. the peak of activity was reached at 10(-7) m. histamine release by mcaf was rapid with an initial rate comparable with histamine release by an optimal dose of anti-ige. mcaf led to peak histamine release within 1 min. 80% of the subjects tested were responsive to mcaf or anti-ige, while all were responsive to fmlp. the percent ... | 1992 | 1370686 |
| termination of second messenger signaling in olfaction. | by using isolated rat olfactory cilia and a fast kinetics methodology, it has been demonstrated that odorant-induced second messenger signaling in the millisecond time range is terminated via phosphorylation reactions catalyzed by specific protein kinases. the cyclic adenosine nucleotide pathway is turned off by kinase a activity, whereas the inositol trisphosphate cascade is terminated by kinase c. the data support the concept that desensitization of odorant responses involves phosphorylation o ... | 1992 | 1370581 |
| characterization of guanosine diphospho-d-mannose dehydrogenase from pseudomonas aeruginosa. structural analysis by limited proteolysis. | alginate is believed to be a major virulence factor in the pathogenicity of pseudomonas aeruginosa in the lungs of patients suffering from cystic fibrosis. guanosine diphospho-d-mannose dehydrogenase (gdpmannose dehydrogenase, ec 1.1.1.132) is a key enzyme in the alginate biosynthetic pathway which catalyzes the oxidation of guanosine diphospho-d-mannose (gdp-d-mannose) to gdp-d-mannuronic acid. in this paper, we report the structural analysis of gmd by limited proteolysis using three different ... | 1992 | 1370473 |
| characteristics of a glycoprotein in the ocular surface glycocalyx. | a monoclonal antibody has been produced that binds to the apical squames (flattened cells) of the rat ocular surface epithelium and to the goblet cells of the conjunctiva. immunoelectron microscopic localization of the antigen indicates that in apical cells it is present along the apical-microplical membrane in the region of the glycocalyx. in subapical squames, the antigen is in cytoplasmic vesicles. in some goblet cells, the antigen is in the golgi network, and in others, it is located primari ... | 1992 | 1370440 |
| activation of nk cells in mice following corneal infection with herpes simplex virus type-1. | natural killer (nk) cells are large granular lymphocytes that mediate antigen nonspecific, non-major histocompatibility complex (mhc) restricted lysis of virus infected cells. they are thought to play a role in innate resistance to herpes simplex virus (hsv) infections. in most animal studies reported to date, the virus was injected intraperitoneally, not a natural route of infection. using a murine model of acute hsv-1 ocular infection, we demonstrate that increased splenic nk activity is induc ... | 1992 | 1370438 |
| fluctuations in continuous mammalian cell bioreactors with retention. | continuous flow bioreactors with cell retention have been increasingly used for the cultivation of mammalian cells. the potential advantages of such bioreactors are high cell concentrations and volumetric productivities. in many reported cases, these systems have shown fluctuations in cell concentrations of various frequency and magnitude. to analyze the dynamics of the fluctuations, a model-based approach is followed. simulations showed that large fluctuations in biomass resulted in response to ... | 2005 | 1369221 |