Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| hydroxylation of tyrosine by plant peroxidase and mushroom tyrosinase, with and without hydrazine, to retard the oxidation of dopa. | tyrosine was oxidized to dopa by horseradish peroxidase and mushroom tyrosinase. the first step of hydroxylation of tyrosine in the synthesis of melanin was demonstrated by isolation of dopa from the reaction mixture using hydrazine as a selective retardant. | 1977 | 410043 |
| complementary laminar terminations of afferents to area 17 originating in area 18 and in the lateral geniculate nucleus in squirrel monkey. | the projections from area 18 and the lateral geniculate nucleus onto area 17 of the squirrel monkey (saimiri) were investigated with retrograde (horseradish peroxidase) and anterograde (tritiated proline) labelling techniques, and the (fink-heimer) silver impregnation method for degenerating axons and their terminals. the association fibers from area 18 terminated in all layers of area 17 except in layer iv and in the lower aspect of layer iiic. the greatest number of terminals were in layers i, ... | 1977 | 409740 |
| mannose-binding hemagglutinins in extracts of pseudomonas aeruginosa. | mannose-binding hemagglutinins were found in the extracts of a pyocyanin-forming pseudomonas aeruginosa, which contain galactose-specific hemagglutinins. they were purified simultaneously with the latter proteins by heating to 70 degrees c, precipitating with ammonium sulfate, application to a sepharose 4b column, and elution from it by 0.05 m mannose. the mannose-specific hemagglutinins were shown to be similar to the galactophilic ones in (a) being glycoproteins of very low molecular weight (a ... | 1977 | 409463 |
| [effect of chemical modification on thermal stability of horseradish peroxidase]. | soluble preparations of horse radish peroxidase are obtained by means of its amino groups modification with glutaric aldehyde, maleic anhydride and inert proteins including albumin. the enzyme activity is found to decrease under the modification with glutaric aldehyde and to be unchanged at all other cases. thermal stability of the enzyme preparations obtained is studied within the temperature range from 56 to 80 degrees c. thermostability of glutaric aldehyde-modified peroxidase is approximatel ... | 1977 | 409438 |
| permeability properties of the tissues in the optic nerve head region in the rabbit and the monkey. an ultrastructural study. | the distribution of the protein tracer, horseradish peroxidase, has been studied in the optic nerve head region in rabbits and monkeys by light and electron microscopy. following intravenous injection of the tracer, the eyes were enucleated after varying time intervals. leaking out of the choroidal capillaries in the peripapillary choroid, peroxidase rapidly spread to the adjacent sclera and to the intraneural connective tissue, that is lamina cribrosa and the connective tissue surrounding the i ... | 1977 | 409092 |
| [thermal inactivation and storage behavior of technologically important enzymes. iii. effect of reagents added to peroxidase and lipoxygenase]. | the influence of milieu factors on the thermal inactivation of peroxidase and lipoxigenase was investigated. cationogenic, anionogenic, non-ionogenic and amphoteric tensides were more or less effective in inactivating horseradish peroxidase. most effective in this respect were lecithine and monoglyceride, both capable of swelling in water. in presence of lecithine, peroxidase was inactivated already at 0 degrees c and ph 4.0. linoleic acid was more efficient in an oxygen stream than in presence ... | 1977 | 408986 |
| cells of origin and terminal distribution of descending projections of the rat somatic sensory cortex. | the retrograde, horseradish peroxidase technique has been used to demonstrate the cells of origin of corticofugal fiber systems arising in the rat somatic sensory cortex and projecting to the striatum, diencephalon, brainstem, and spinal cord. correlative experiments conducted with the anterograde, autoradiographic method have been used to confirm the terminal distribution of many of these fiber systems. corticofugal pathways directed to subcortical structures arise in the first and second somat ... | 1977 | 408380 |
| peroxidase permeability of the cryogenic chorioretinal adhesion. | under normal conditions the retinal pigment epithelium does not permit passage of peroxidase tracers. photocoagulation disrupts this barrier. rhesus monkeys which received transconjunctival cryotheropy showed a similar disruption, with leakage of horseradish peroxidase into the retina. this leakage persisted for the 6-month duration of the experiment. one month after treatment, a firm chorioretinal adhesion was present, characterized by gliosis, adherent junctions, and cellular interdigitation. ... | 1977 | 408292 |
| cortical cells projecting to the dorsal column nuclei of rhesus monkeys. | corticals cells projecting to the dorsal column nuclei (dcn) of rhesus monkeys have been identified after unilateral or bilateral injection of horseradish peroxidase (hrp) into the dorsal medulla. hrp-positive neurons identifiable as the source of cortico-dcn projections were pyramidal cells in layer v whose largest diameters ranged from 12--31 micron. cortico-dcn neurons were concentrated in the trunk, fore- and hindlimb regions of area 4 and of si, and to a lesser extent in sii. this distribut ... | 1977 | 408162 |
| prefrontal granular cortex of the rhesus monkey. ii. interhemispheric cortical afferents. | in 6 adolescent rhesus monkeys, horseradish peroxidase (hrp) was injected into 6 regions of the dorsalateral convexity of the prefrontal granular cortex. the commissural connections originated in both homotopical and heterotopical zones of the hemisphere contralateral to the injection site. the areas affected by the injections, i.e. areas 46,45, 10, 9, 12 and 8a, received extensive homotopical interhemispheric input. hrp-labeled neurons were less extensive in heterotopical as opposed to homotopi ... | 1977 | 407976 |
| optic disc edema in raised intracranial pressure. iii. a pathologic study of experimental papilledema. | optic nerve heads of 21 eyes from 13 rhesus monkeys that had developed papilledema secondary to chronically raised intracranial pressure from balloon implantation in the subarachnoid space were studied by light and electron microscopy. nine eyes were also examined with the horseradish peroxidase tracer technique. the pathologic changes in the optic nerve head included severe axonal changes and mild interstitial edema. axonal alteration ranged from axonal swelling to formation of cytoid bodies. i ... | 1977 | 407894 |
| foveal sparing. new anatomical evidence for bilateral representation of the central retina. | the pattern of retinal ganglion cell projections to the dorsal lateral geniculate nuclei has been demonstrated with horseradish peroxidase neuronography. a 1 degree-wide strip centered on the vertical meridian has been found in which ipsilaterally and contralaterally projecting ganglion cells intermingle. this strip expands to a width of 3 degrees at the fovea, since mixing of horseradish peroxidase-labeled and unlabeled ganglion cells was found in a band approximately 0.5 degrees wide along bot ... | 1977 | 407893 |
| the morphology and laminar distribution of cortico-pulvinar neurons in the rhesus monkey. | using autoradiography and the horseradish peroxidase method, the morphology and laminar distribution of cortico-pulvinar neurons and the reciprocity of connections between pulvinar and cortex were examined in five rhesus monkeys which had received medial, lateral and inferior pulvinar nucleus injections of both tritiated amino acids and horseradish peroxidase. cortico-pulvinar neurons were identified in one heterotypical cortical area (area 17) and in many homotypical areas in frontal (areas 45, ... | 1977 | 407094 |
| spinal neurons project to the dorsal column nuclei of rhesus monkeys. | cells of origin of ascending nonprimary afferents to the dorsal column nuclei of rhesus monkeys have been identified in the spinal cord by the retrograde transport of horseradish peroxidase. these neurons are mainly located in lamina iv and medially in more ventral laminae of the dorsal horn on the side ipsilateral to the medullary injection. large neurons in the ventral horn ("spinal border cells") also appear to project to the ipsilateral dorsal medulla. the dorsal column nuclei of a primate t ... | 1977 | 404704 |
| experimental macular edema after lens extraction. | macular edema has been observed frequently in man after cataract extraction, but pathogenic mechanisms remain unclear. seven eyes of four young adult rhesus monkeys underwent lens extraction. the retinas and maculae of these eyes were examined by ophthalmoscopy fundus photography, fluorescein angiography, light and electron microscopy, and the horseradish peroxidase tracer technique. in the macular region, the blood-retinal barrier at the retinal vasculature was disrupted in three of the seven e ... | 1977 | 404267 |
| ultrastructural investigations on surface structures involved in coxiella burnetii phase variation. | by using the cytochemical staining procedure with concanavalin a, horseradish peroxidase, and diaminobenzidine, no surface carbohydrates with terminal alpha-glucosyl or sterically closely related residues could be detected on the cell walls of coxiella burnetii phases i and ii. using a polycationized ferritin derivative as a cytochemical probe, anionic binding sites were visualized in the electron microscope on cell membranes of c. burnetii phase ii, but not on phase i organisms. the sites appea ... | 1977 | 404251 |
| pulvinar-latero posterior afferents to cortical area 7 in monkeys demonstrated by horseradish peroxidase tracing technique. | pulvinar-latero posterior afferents to the parietal cortical area 7 of the monkey have been demonstrated by means of horseradish peroxidase (hrp) tracing technique. following hrp injection of area 7, labelled neurons have been found in the pulvinar medialis and the nucleus lateralis posterior. the role of these pulvinar projection fibers is discussed with reference to the "visual neurons" of area 7 recently recorded from. | 1977 | 404172 |
| plant glycoprotein biosynthesis. uridine diphosphate n-acetyl-glucosaminyltransferase from horseradish root. | a particulate enzyme preparation from horseradish root tissue was shown to catalyze the transfer of 2-acetamido-2-deoxy-d-[14c1]glucose from uridine diphosphate 2-acetamido-2-deoxy-d-[14c1]glucose to an exogenous acceptor molecule derived from horseradish peroxidase. the acceptor was produced from purified peroxidase by the action of a mixture of glycoside hydrolases covalently bound to sepharose. the membrane preparation containing the transferase was purified approximately 12-fold by aqueous t ... | 1977 | 403958 |
| cells of origin and terminal distribution of corticostriatal fibers arising in the sensory-motor cortex of monkeys. | the cells of origin of the corticostriatal projection have been identified in squirrel monkeys by the use of the retrograde horseradish peroxidase method. in the subfields of the somatic sensory, motor, parietal and frontal areas of the cortex, cells projecting to the ipsilateral striatum are relatively sparsely distributed and form a group of small- to medium-sized pyramidal cells with an average somal diameter from area to area of 14-16 mum. such cells are found only in layer v of the cortex ( ... | 1977 | 403206 |
| efferent connections of the hippocampal formation in the rat. | in this investigation the projections of the hippocampal formation to the septal area and hypothalamus were studied in the rat with the combined use of 3h-amino acid radioautography and horseradish peroxidase histochemistry. the results indicate that all of the fibers which project to the hypothalamus and the majority of fibers which project to the septum arise from the subicular cortex and not from hippocampal pyramidal cells. the projection to both of these areas are topographically organized ... | 1977 | 402984 |
| heterogeneous afferents to the inferior parietal lobule of the rhesus monkey revealed by the retrograde transport method. | the sources of afferent connections to the inferior parietal lobule (rostral part of the area 7 of brodman; pf and rostral part of pg of von bonin and bailey) were examined with the retrograde transport method in infant and adult rhesus monkeys. two to 3 days after injections of horseradish peroxidase (hrp) into the cortex, the animals were anesthetized, and the brains fixed and processed for the histochemical demonstration of the enzyme marker. labeled neurons were found in layer iii in the ips ... | 1977 | 402983 |
| cyclocryotherapy: a light and electron microscopic study. | previous anatomic studies of the effects of cyclocryotherapy have produced confusing results because of variations in technique and instrumentation. five eyes from three patients treated by a standard technique were compared to the eyes of rhesus monkeys that received identical treatment. the monkeys were given horseradish peroxidase intravenously 30 minutes before enucleation. hemorrhage, edema, and epithelial disruption were characteristic of the acute lesions. later, the nonpigmented ciliary ... | 1977 | 402130 |
| demonstration of bilateral projection of the central retina of the monkey with horseradish peroxidase neuronography. | in the primate, ganglion cells of the temporal retina project ipsilaterally and those of the nasal retina, contralaterally into the optic tract. the vertical meridian passing through the fovea defines the border between these two populations of ganglion cells and has been demonstrated in four macaque monkeys after unilateral injection of horseradish peroxidase into the dorsal lateral geniculate nucleus and examination of the pattern of retrograde labeling of those ganglion cells projecting to th ... | 1977 | 401836 |
| displaced ganglion cells in the retina of the monkey. | horseradish peroxidase has been injected into the dorsal lateral geniculate nucleus of macaque monkeys to label ganglion cells of the retina by retrograde axoplasmic transport. displaced ganglion cells, with somata in the inner nuclear layer and dendrites in the inner plexiform layer, were detected by virtue of their filling with peroxidase-positive granules. these cells were numerous in the peripapillary region, but relatively uncommon elsewhere in the retina. | 1977 | 401780 |
| immunohistologic localization of antigen and antibody in antigen-induced arthritis using horseradish peroxidase (hpo) labeling. | 1978 | 401418 | |
| oxidation of tryptophan-p-1 and p-2 by beef liver catalase-h2o2 intermediate: comparison with horseradish peroxidase. | tryptophan-p-1 (trp-p-1) and p-2, which have high mutagenic and carcinogenic potential, are oxidized by catalase- and horseradish peroxidase (hrp)-h2o2 intermediates with optimum ph 5.9 (0.2 m-acetate) in catalase and ph 5.0 (0.2 m-acetate), 8.0 (0.01 m-phosphate) in hrp. the rate constants (k4) of the oxidation in the catalase at ph 5.9 (0.2 m-acetate) were 2965 m-1 x sec-1 for trp-p-1 and 576 m-1 x sec-1 for trp-p-2. in the case of hrp, 1894 m-1 x sec-1, (ph 5.0, 0.2 m-acetate) for trp-p-1 and ... | 1979 | 399746 |
| the retrograde transport of horseradish peroxidase: problems in the interpretation of its ultrastructural aspects. | in this study we consider critically the use of hrp in research on the ultrastructure of the central nervous system. in fact, although controls make by optical microscope on semi-thin sections made us certain that some of the neurons were definitely marked with the enzyme, when the same specimen was observed by electron microscope, in no case was it possible for us to distinguish any particular aspect of the ultrastructural morphology of labelling within neurons. | 1979 | 399735 |
| sulphydryl oxidase: oxidation of sulphydryl groups and the formation of three-dimensional structure in proteins. | sulphydryl oxidase, an enzyme isolated from milk, catalyses the de novo synthesis of disulphide bonds. thiol groups in amino acids or their derivatives, peptides, and proteins are oxidized; molecular oxygen serves as the electron acceptor and undergoes a two-electron reduction to hydrogen peroxide. michaelis constants vary considerably amongst various substrates; glutathione is a particularly good substrate. inhibition studies and oxidation of 1,3-diphenylisobenzofuran suggest a mechanism involv ... | 1979 | 398763 |
| a simple and rapid section embedding technique for sequential light and electron microscopic examination of individually stained central neurons. | a method for section embedding of central nervous tissue, and its application to light and electron microscopic examination of neurons stained by intracellular injection of horseradish peroxidase, is described. after primary fixation in aldehydes, thick (10--80 micrometer) sections are cut on a vibratome. they are then treated for the histochemical demonstration of peroxidase, postfixed in osmium tetroxide, dehydrated and infiltrated with spurr's low viscosity resin. infiltrated sections are emb ... | 1979 | 397378 |
| bacterial lipopolysaccharides bind selectively to lymphocytes from lipopolysaccharide high-responder mouse strains. | three different concentrations of horseradish peroxidase-labelled lipopolysaccharide (lps-hrp) were added in vitro to spleen cells from the lps high-responder strain c3h/tif and to cells from the low-responder strain c3h/hej. after being washed and fixed the cells were exposed to the substrate and prepared for electron microscopy. after addition of 7 and 0.7 microgram/ml of labelled lps only lymphocytes from the high-responder strain were labelled. about 5-10% of the cells from c3h/tif bound lps ... | 1979 | 396666 |
| renal reabsorption and excretion of horseradish peroxidase. | 1979 | 393894 | |
| insulin-induced receptor loss in the cultured human lymphocyte: quantitative morphological perturbations in the cell and plasma membrane. | when cultured human lymphocytes (im-9) are exposed to 10(-6) m procine insulin for 6 h, washed, and incubated with 125i-insulin, the ability of the cell to bind the labelled hormone is reduced by a mean of 78%. under these experimental conditions that induce insulin-receptor loss in this cell there is a mean 95% increase in microinvaginations in the plasma membrane revealed by electron microscopy on freez-fractured replicas of the cell. at the same time, horseradish peroxidase uptake, a marker o ... | 1979 | 393710 |
| [cytochemical and ultrastructural studies of the cell wall of candida albicans (author's transl)]. | the ultrastructural localization of polysaccharides in the cell wall of candida albicans was carried out by means of concanavalin a on glycol methacrylate ultrathin sections. the sections were incubated successively with horseradish peroxydase, 3-3' diaminobenzidine and h2o2, for revealing the binding sites of the lectin. this method allowed us to localize mannan, since concanavalin a does not react with other polysaccharides of the c. albicans cell wall. in these conditions mannan was found to ... | 1979 | 393198 |
| igg molecules, fab' fragments and igg-peroxidase conjugates as markers in replicas of deep-etched, labeled cell surfaces. | sheep red blood cell (srbc) ghosts were incubated with preparations of anti-srbc igg, antigen-binding fragments (fab') or igg coupled to horseradish peroxidase (hrpo). frozen samples of the labeled ghosts were deep-etched and replicated with platinum-carbon to visualize their surface features in the transmission electron microscope. an analysis of both size and density of surface "particles" observed on labeled ghosts was performed to evaluate which markers could be practically used to label the ... | 1979 | 392717 |
| a solid-phase enzyme-linked immunosorbent assay (elisa) for detection of antibody to rubella virus. | an enzyme-linked immunosorbent assay was established for detection of antibodies to rubella virus. in this system commercially available rubella antigen was attached to the wells of polystyrene microtitre plates after which sera were added and incubated to allow the formation of antigen-antibody complexes. the presence of bound antibody was detected by adding anti-human globulin coupled to horseradish peroxidase and visually observing the colour change produced after addition of an appropriate s ... | 1979 | 392423 |
| evaluation of igg molecules, fab' fragments and igg-horseradish peroxidase conjugates as surface labels for freeze-etched membranes. | sheep red blood cell (srbc) ghosts were incubated with preparations of anti-srbc igg, antigen-binding fragments of igg (fab') or igg coupled to horseradish peroxidase (hrpo). frozen samples of the labelled ghosts were deep-etched and replicated with platinum-carbon to visualize their surface features in the transmission electron microscope. the surfaces of control ghosts contain a very low number of 'background' particles (42 +/- 8 particles/micron 2) that vary in size from 4.5 to 25 nm. after l ... | 1979 | 392105 |
| morphological characteristics of lateral rectus motoneurones shown by intracellular injection of hrp. | the morphology of identified lateral rectus motoneurones is described after staining by intracellular iontophoresis of horseradish peroxidase. soma vary in shape and size according to the number and orientation of primary dendrites. the basic pattern of arborisation shows short primary dendrites which branch close to the soma, forming a distal ramification extending over 600 to 1,200 micrometer from the soma. distal dendrites extend into the ipsilateral medial vestibular nucleus, the reticular f ... | 1979 | 392076 |
| projections of lamprey spinal neurons determined by the retrograde axonal transport of horseradish peroxidase. | the spinal cords of larval sea lampreys (petromyzon marinus) and adult river lampreys (ichthyomyzon unicuspis) were injected with horseradish peroxidase through a transection 1 cm caudal to the last gill. some animals also had a spinal hemisection 1 cm caudal to the injection. after recovery periods of 1 to 52 days, the spinal cords were treated with diaminobenzidene and hydrogen peroxide, and the projections of various cell types determined in wholemount slides. from these observations the foll ... | 1979 | 391835 |
| enzyme immunoassay of testosterone in plasma, with use of polyethylene glycol to separate antibody-bound and free hormone. | we describe an enzyme immunoassay for testosterone in which we use a testosterone-3-(carboxymethyl)oxime horseradish peroxidase conjugate as the label and an antiserum, raised in rabbits, to testosterone-3-(carboxymethyl)oxime-bovine serum albumin conjugate. polyethylene glycol (carbowax 6000) is used to separate antibody-bound and free steroid. the assay has a sensitivity of 12 pg/assay tube and satisfies the usual criteria of specificity, precision and accuracy. the results agree well with tho ... | 1979 | 390778 |
| competitive enzyme-linked immunoassay for factor viii antigen. | we describe a competitive enzyme-linked immunoassay for factor viii antigen. binding of anti-factor viii to solid-phase factor viii antigen is competitively inhibited by the free factor viii antigen that is to be measured. the amount of anti-factor viii bound to solid-phase viii is measured by applying in sequence a heterologous bridging antibody and a soluble antibody/enzyme immune complex. the soluble complex used was rabbit antiperoxidase/horseradish peroxidase. peroxidase activity is inverse ... | 1979 | 387293 |
| vestibular neuroanatomy: recent observations. | the modern neuroanatomical technique of using a retrograde axoplasmic tracer (horseradish peroxidase) to label neurons has aided the revelation of several important connections in the vestibular system. the organization of the oculomotor nucleus and the existence of an interneuron in the abducens nucleus have importance in understanding some ocular disorders. a detailed description of the location of vestibulo-ocular neurons to individual extraocular muscles is now available which may provide a ... | 2017 | 386889 |
| cytochemical localization of lectin labeled vesicles in gerl region of hepatoma ascites cells. | the fate of lectin labeled internalized plasma membrane in the ascites tumor form of the chang rat hepatoma growing under in vivo and in vitro conditions was investigated cytochemically. ascites cells were incubated in convanavalin a (con a) and horseradish peroxidase (po), either with or without prior glutaraldehyde fixation and subsequently treated with 3',3-diaminobenzidine. in cells fixed before con-a-po labeling the reaction product was localized as a continuous and even layer upon the exte ... | 1979 | 385567 |
| topographical origin and ganglion cell type of the retino-pulvinar projection in the cat. | in order to examine the pattern of the retino-pulvinar projection in the cat, the existence of which has been recently demonstrated using autoradiographic fiber tracing technique, a small amount of horseradish peroxidase (hrp) was injected into the lateral part of the pulvinar nucleus at various rostocaudal levels. the retrogradely labeled ganglion cells were analyzed in terms of their topographical location and cell size, as seen inretinal whole mounts. the results were compared with those obta ... | 1979 | 385107 |
| descending pathways of the nucleus of the optic tract in the rat. | the descending pathways of the nucleus of the optic tract (pars lateralis) (notl) in the rat were examined in nauta-gygax impregnated sections and further confirmed by the horseradish peroxidase labeling technique. following stereotactic lesions in the notl, visual cortex, and superior colliculus, projections which orginate in the notl were identified by elimination of the tectofugal and tectopetal fibers of the superior colliculus. the descending fibers from the notl appeared to be organized in ... | 1979 | 385106 |
| [purification of commercial horseradish peroxidase for the use in enzyme immunoassay]. | 1979 | 384697 | |
| rat cns in experimental chronic serum sickness: integrity of the zonulae occludentes of the choroid plexus epithelium and brain endothelium in experimental chronic serum sickness. | the present study is an extensive systematic immunofluorescence and labelled electron microscopic investigation of chronic rat serum sickness brains. it is specifically directed at discerning effects of chronic serum sickness upon the zonulae occludentes of both the choroid plexus epithelium and the intra-cerebral endothelium. although the choroid plexus is a known site of complex entrapment in systemic immune complex disorders, comprehensive immune studies of the cns parenchymal vasculature hav ... | 1979 | 384287 |
| effects of isoproterenol on the dense core and perigranular membrane of atrial specific granules. | following subcutaneous injections of isoproterenol hydrochloride (iso), atrial cells present a large number of partly degranulated or completely clear "specific granules" enclosed by an intact membrane. such profiles were never encountered in normal controls and might suggest iso-induced release of a secretory product. permeability of perigranular membrane was tested using the extracellular macromolecular tracer horseradish peroxidase (hrp). reaction product was entirely absent within granules o ... | 1979 | 383301 |
| tamm-horsfall glycoprotein: ultrastructural immunoperoxidase localization in rat kidney. | tamm-horsfall urinary glycoprotein (th) is the primary constituent of urinary casts. the intracellular distribution of th in normal rat kidney was determined by immunoelectron microscopy using horseradish peroxidase-labeled antibodies and compared with morphologic localization of th by immunofluorescence and light immunoperoxidase microscopy. electron microscopy revealed an intracellular localization of th restricted to the thick ascending limb of the loop of henle (alh). in this nephron segment ... | 1979 | 379435 |
| solid-phase enzyme immunoassay for serum ferritin. | we describe a solid-phase enzyme immunoassay for ferritin in human serum, with use of a horseradish peroxidase-labeled antibody and a highly sensitive chromogen, 2,2'-azino-di(3-ethyl-benzthiazoline-6-sulfonate). the assay requires only 10 microl of serum per assay, relatively less time and labor than other assys for ferritin, and as little as 10 pg of ferritin can be measured. we believe this assay offers a reliable alternative to radioimmunoassay for serum ferritin. | 1979 | 378465 |
| alpha-bungarotoxin-horseradish peroxidase conjugate: preparation, properties and utilization for the histochemical detection of acetylcholine receptors. | a method is presented for the efficient conjugation of horseradish peroxidase to alpha-bungarotoxin. the 1:1 molar conjugate obtained is purified to completion by gel filtration on sephadex g-100, followed by ion exchange chromatography on cm-sephadex. the conjugate retains half of the activity of unmodified horseradish peroxidase and binds effectively to the nicotinic acetylcholine receptor of muscle. the conjugate is proven to be useful reagent for the histochemical staining of the receptor on ... | 1979 | 376692 |
| horseradish peroxidase labeled antisera--a diagnostic method for evaluating the percentages of b lymphocytes in peripheral blood. | horseradish peroxidase labeled antisera supplied commercially was used to evaluate b lymphocytes in peripheral blood. this technique not only gave comparable results to the conventional fluorescein method, but also proved to be more advantageous. | 1979 | 376687 |
| a sensitive solid phase enzymeimmunoassay for testosterone in plasma and saliva. | a sensitive, solid phase enzymeimmunoassay suitable for determining testosterone concentrations in samll aliquots of plasma (20 microliter) and saliva (200 microliter) has been developed. a solid phase antiserum raised against a testosterone-11 alpha-hemisuccinate/bovine serum albumin conjugate was prepared by coupling to cyanogen bromide activated cellulose. the "enzyme label" was a covalently linked testosterone/horseradish peroxidase conjugate. the assay had a lower limit of sensitivity of 4p ... | 1979 | 375481 |
| enzyme immunoassay of estrogen-like substances in plasma, with polyethylene glycol as precipitant. | we devised an enzyme immunoassay for plasma estrogen-like substances in pregnancy, using estriol covalently linked to horseradish peroxidase. free and bound steroid were separated by use of polyethylene glycol reagent. the assay satisfies the normal criteria of specificity and precision. results obtained by radioimmunoassay and enzyme immunoassay agreed well. our assay is particularly applicable in smaller clinical laboratories to routine determination of plasma estriol in pregnancy. | 1979 | 373925 |
| detection of antibody-forming cells and humoral antibody to horseradish peroxidase. | two new simple methods for detecting antibody-forming cells by hemolytic plaque assay and hemagglutinating antibody to horseradish peroxidase have been developed in mice. both techniques utilize as target, sheep erythrocytes coupled directly with horseradish peroxidase. these assays are sensitive, antigen-specific and are useful to quantitate both direct and indirect antibody-forming cells and humoral antibodies. | 1979 | 372446 |
| an evaluation of the immunoperoxidase technique in renal biopsy diagnosis. | an evaluation of the use of horseradish peroxidase labelled antisera against igg, igm, iga and c3 compared with corresponding antisera labelled with fluorescein isothiocyanate has been carried out on renal biopsy material from 200 patients with renal disease. a disparity between the two methods was observed in 10-19% of the readings according to the antigen being demonstrated. the majority of differences recorded would not have influenced the executive diagnosis. where the immunological results ... | 1979 | 371888 |
| [thalamocortical projections of the anterior ventral nucleus: study using retrograde axonal transport of horseradish peroxidase]. | 1978 | 371568 | |
| luminescence immunoassay (lia): a solid-phase immunoassay monitored by chemiluminescence. | a luminescence immunoassay (lia) has been developed utilizing the chemiluminescent luminol reaction with heme as catalyst. rabbit antibody against human serum albumin was quantitated in antigen coated plastic tubes using commercially available goat anti-rabbit igg conjugated to horseradish peroxidase which was the source of heme. the measurable range of antibody is considerably wider by lia than by enzyme immunoassays. the time to develop and measure activity is short and constant which makes li ... | 1979 | 370310 |
| electron-microscopic demonstration of streptococcus mutans in 5-day-old experimental dental plaque using immunohistochemistry. | the present work described the distribution of horseradish peroxidase reaction product obtained after immunohistochemical staining of consecutive ultra-thin sections of 5-day-old experimental dental plaques using differently absorbed anti-s.mutans sobrinus, anti-s.mutans mutans, and normal rabbit lambda-globulins. it was found that an in situ identification of s.mutans subspecies sobrinus could be obtained using thoroughly absorbed anti-s.mutans sobrinus gamma-globulins and consecutive sections. ... | 1978 | 369295 |
| comparison of light-microscopic methods for visualizing horseradish peroxidase granules in stained and unstained tissue. | this study was directed toward a comparison of the effectiveness of three different light-microscopical methods for identifying and studying the features of cells that contain horseradish peroxidase (hrp) granules. the cells studied were located in the trigeminal ganglia and were labeled after the injection of hrp solutions into single, maxillary, canine teeth. the tissue was examined with light field, dark field, and differential interference optical systems. the results indicated that differen ... | 1978 | 368347 |
| enzyme-immunoassay of human placental lactogen. | an enzyme-immunoassay (eia) for estimation of human placental lactogen (hpl) in plasma or serum was developed using hpl labelled with horseradish peroxidase (hrp) and anti-hpl sera raised in rabbits. the separation between antiserum-bound and free labelled hormone was accomplished with a double antibody solid phase technique. interference of substances from the sample with the immunoassay was prevented by dilution of the sample with at least a factor of 20 and measurement of the labelled hormone ... | 1979 | 367637 |
| effect of histamine and methacholine on guinea pig tracheal permeability to hrp. | the effects of histamine, methacholine, and ether on the permeability of the respiratory mucosa to macromolecules were investigated employing a radioimmunoassay and histochemical techniques to monitor movement of horseradish peroxidase (hrp) from airway lumen to blood. we found that 0.08% of the dose of hrp instilled into guinea pig tracheas was present in the blood volume at 10 min, and plasma hrp levels increased at a rate of 0.0036% instilled dose/min thereafter. after inhalation challenge, s ... | 1978 | 365840 |
| an improved enzyme immunoassay for progesterone in human plasma. | we describe an enzyme immunoassay for progesterone in which we use a progesterone-11alpha-hemisuccinyl-horseradish peroxidase conjugate as the "label" and an antiserum raised in rabbits to a progesterone-11alpha-hemisuccinyl-bovine serum albumin conjugate. in this assay, antibody-bound and free steroid are separated by using a second antibody precipitation procedure. the assay has a lower limit of sensitivity of 10 pg/assay tube and satisfies the usual criteria of specificity, precision, and acc ... | 1978 | 363304 |
| incorporation of rat histocompatibility (agb) antigens into liposomes, and their susceptibility to immune lysis. | the purification of detergent-solubilized rat histocompatibility antigens is described. the antigen may readily be incorporated into synthetic vesicles (liposomes) which appear to be unilamellar and between 0.1 microbeter and 0.2 micrometer in diameter. the addition of specific antibody leads to the agglutination and precipitation of the liposomes. an enzyme, horseradish peroxidase, may be incorporated into the trapped aqueous phase of the liposome, and its release by antibody and complement can ... | 1978 | 361419 |
| enzyme immunoassay for total oestrogens in pregnancy plasma or serum. | we have developed an enzyme immunoassay for total oestrogens in pregnancy serum of plasma, using horseradish peroxidase (hrp) as the marker enzyme. a test combination consisting of an antiserum against oestriol-16/17-monosuccinyl-albumin and oestriol-16/17-monosuccinyl-hrp yielded a sensitive system, which reacted to approximately the same extent with oestrone, oestradiol, oestriol and their 16- and 17-conjugates. samples had to be diluted 1 to 10 to avoid interference of plasma factors with the ... | 1978 | 361293 |
| the defined antigen substrate sphere system with direct immunohistoperoxidase for detection of soluble dengue antigen in sera of patients with dengue hemorrhagic fever. | the defined antigen substrate sphere system is a simple method for detecting antigen or antibody in the circulation. the technic is based on the coupling of antigen or antibody with sepharose 4b beads that have been activated by cyanogen bromide. in this study the activated beads were exposed to dengue antigen in the serum from a patient with dengue hemorrhagic fever and then stained with antidengue antibody conjugated with horseradish peroxidase. the positive reaction showed brown beads by ligh ... | 1978 | 360820 |
| a sensitive manual enzyme immunoassay for thyroxine. | we describe an immunoassay for thyroxine in serum. in the assay specific antibody covalently bonded to latex particles is used, along with horseradish peroxidase as the label, and o-phenylenediamine as the chromogen. the flexible protocol is designed for manual execution. performance is similar to that of the highest-sensitivity thyroxine radioimmunoassays. results correlate well with radioimmunoassay (r = 0.99, slope = 0.93, y-intercept = 2.4 microgram/liter for 201 samples) and an automated en ... | 1978 | 359195 |
| a simple device for pressure injections of horseradish peroxidase into small central neurones. | 1978 | 359104 | |
| use of horseradish peroxidase to block nonspecific enzyme uptake in immunoperoxidase microscopy. | intrinsic tissue peroxidase activity can be more or less successfully destroyed by methanol-h2o2 treatment. it has been found, however, in our laboratory that horseradish peroxidase (hrp) coupled to antibody will bind to some tissue components on a nonspecific basis and remain to take part in the histochemical stain. this contributes considerably to the background. this difficulty can be largely overcome if the tissues are pretreated with a solution of horseradish peroxidase which binds with non ... | 1978 | 357648 |
| a morphologic and permeability study of luminal smooth muscle cells after arterial injury in the rat. | in the carotid air drying model of arterial endothelial injury in the stressed rat, endothelium does not always regenerate over the zone of intimal thickening; instead, a layer of modified smooth muscle cells appears to form a temporary luminal surface. we examined the properties of these luminal smooth muscle cells in injured right carotid arteries from stressed rats at intervals up to 2 months by light, scanning electron, and transmission electron microscopy. before perfusion fixation, selecte ... | 1978 | 355725 |
| histochemical differentiation of complex carbohydrates with variants of the concanavalin a-horseradish peroxidase method. | various treatments carried out prior to the concanavalin a-horseradish perioxidase (hrp) method have been found to affect the staining and have permitted differentiation of three main classes of complex carbohydrates in the rat alimentary tract. class i mucosubstances lose and class ii and iii paradoxically gain concanavalin a-horseradish peroxidase reactivity after periodate oxidation. class ii mucosubstances lose whereas class iii retain or increase their reactivity with a reduction step inter ... | 1978 | 351046 |
| characterization of various nervous tissues of the chick embryos through responses to chronic application and immunocytochemistry of beta-bungarotoxin. | beta-bungarotoxin (beta-bt) was applied to chick embryos at 3-day intervals beginning on the fourth day of incubation to investigate ultrastructurally the effects of chronically and massively applied beta-bt on various nervous tissues and muscles. on the twenty-first day of incubation, spinal cords of beta-bt treated embryos were conspicuously decreased in size. ventral root fibers dorsal root fibers, white matter, and motor neurons disappeared. although spinal ganglia and sympathetic trunk gang ... | 1978 | 350914 |
| cerebellar afferent projections from the vestibular nuclei in the cat: an experimental study with the method of retrograde axonal transport of horseradish peroxidase. | details of cerebellar afferent projections from the vestibular nuclei were investigated by the method of retrograde axonal transport of horseradish peroxidase (hrp) in the cat. the distribution of labeled cells in the vestibular nuclei following hrp injections in various parts of the cerebellum indicates that vestibular neurons in the medial and descending nuclei and cell groups f and x project bilaterally to the entire cerebellar vermis, the flocculus, the fastigal nucleus and the anterior and ... | 1978 | 350598 |
| brain stem neurons projecting to neocortex: a hrp study in the cat. | the cortical projections of the brain stem were investigated in detail in the cat by means of the horseradish peroxidase (hrp) retrograde axonal transport. most of the cells providing ascending fibers to the neocortex were located in the pons (locus coerulus and related structures, central gray substance, dorsal tegmental nucleus, raphe nuclei, reticular nuclei); labeled neurons were also identified in the mesencephalon, mainly in the periaqueductal gray and in the nucleus linearis rostralis. th ... | 1978 | 350597 |
| immunocytological localization of estrogen in human mammary carcinoma cells by horseradish--anti-horseradish peroxidase complex. | tissues from 25 female and 2 male patients of mammary carcinoma were examined by the horseradish--anti-horseradish peroxidase complex to localize the presence of estrogen. carcinoma cells of 10 females and 2 male patients showed positive staining by this technique. the nucleus and cytoplasmic membrane showed the reaction. this method is useful in identifying the exact location of the hormones or hormone-like substances in the malignant cells and, perhaps, will be of great help in selecting the p ... | 1978 | 349272 |
| [immunoelectron microscopy in dermatology]. | the principle of immunoelectronmicroscopic studies using horseradish perpoxidase is described. this method, especially the peroxidase-antiperoxidase multistep technique, reveals more details about the exact localization of immunophenomena in different dermatological diseases. the results of immunological investigations performed on the ultra-structural level in bullous diseases, lupus erythermatosus, vasculitis, and psoriasis are summarized and compared with the immunofluorescent and classical e ... | 1978 | 348650 |
| the central location of the motor neurons to the stapedius muscle in the cat. | in two cats and 10 newborn kittens, retrograde transport of horseradish peroxidase was used to locate the motor neurons of the stapedius muscle. following injection of tracer into the muscle, labeled neurons were observed in the interface between the facial nerve nucleus and the caudal end of the lateral superior olivary nucleus. some marked neurons were also located dorsal and ventral to the facial nucleus. the number of labelled cells varied from a low of 34 to a high of 199. | 1978 | 348268 |
| organization of corticospinal neurons in the cat. | the cells of origin of the corticospinal tract of the cat were identified using the retrograde horseradish peroxidase (hrp) labeling technique. cortical neurons labeled from the spinal cord were confined to layer v and included large betz cells, as well as many smaller neurons of this layer. collections of 5-10 labeled neurons concentrated in areas of 300-500 micrometer diameter were observed, suggesting a columnar-type of organization of corticospinal neurons. injections of hrp into different s ... | 1978 | 348267 |
| the projection of the superior colliculus upon the inferior olivary complex of the cat: an autoradiographic and horseradish peroxidase study. | 1978 | 346175 | |
| binding of antibodies to acetylcholine receptors in electrophorus and torpedo electroplax membranes. | antisera against purified acetylcholine receptors from the electric tissues of torpedo californica and of electrophorus electricus were raised in rabbits. the antisera contain antibodies which bind to both autologous and heterologous receptors in solution as shown by an immunoprecipitation assay. antibodies in both types of antisera bind specifically to the postjunctional membrane on the innervated surface of the intact electroplax from electrophorus electric tissue as demonstrated by an indirec ... | 1978 | 344325 |
| membrane alterations during cornification of mammalian squamous epithelia: a freeze-fracture, tracer, and thin-section study. | tight junctions (zonulae occludentes) create a pericellular barrier to the diffusion of large molecules in non-keratinizing mammalian epithelia. however, in cornifying epithelia such as the epidermis, the importance of tight-junctional elements versus secreted intercellular lipid for barrier function is uncertain. in an attempt to resolve this question, we compared membrane structure in the stratum granulosum and stratum corneum of epidermis, esophagus, and vagina of newborn and adult humans and ... | 1977 | 339780 |
| enzyme immunoassay for hepatitis b and its comparison to other methods. | a solid-phase enzyme immunoassay for the qualitative detection of hepatitis b surface antigenemia is presented and the results compared to the counter-immunoelectrophoresis and radioimmunoassay methods for detection of the same antigen. the enzyme-antibody conjugate was prepared from horseradish peroxidase coupled with antibodies to three sub-types of hepatitis b virus. polystyrene plastic tubes were used as the solid-phase support. the results of the enzyme immunoassay compare favorably with ra ... | 1977 | 336244 |
| absorption of horseradish peroxidase by neonatal pig intestinal epithelium: effect of escherichia coli (055b5) on absorption. | the pathway of macromolecular transport through the neonatal pig small intestinal epithelium was examined, utilizing the cytochemical marker horseradish peroxidase (hrp). the marker was found adsorbed to the apical microvillous of the enterocytes, within apical tubules, and cytoplasmic vacuoles. extracellular absorbed marker assumed a spherical appearance within the subepithelial spaces but was dispersed within the capillary lumens. uptake of hrp into the enterocyte occurred in 48-hour old neona ... | 1977 | 335926 |
| studies on translocation of immunoglobulins across intestinal epithelium. iv. evidence for binding of iga and igm to secretory component in intestinal epithelium. | we conducted studies concerning the issue of whether secretory component (sc) is a specific receptor on intestinal epithelial cells for iga and igm. initially, frozen sections of human intestinal mucosa were incubated with dimeric monoclonal human iga, conjugated to horseradish peroxidase; adjacent sections were reacted with peroxidase-conjugated antibodies to sc. the conjugated iga and anti-sc bound to similar sites in the epithelium, that is to basolateral margins and supranuclear cytoplasm of ... | 1977 | 334623 |
| uptake of horseradish peroxidase by geniculo-cortical axons in the golden hamster: analysis by computer reconstruction. | micro-injections of horseradish peroxidase (hrp) were made into the visual cortex of the golden hamster. the "projection lines" of labelled neurons in the dorsal lateral geniculate nucleus (lgnd) were three-dimensionally reconstructed, using a computer graphics technique. the lines run rostrally and medially from their origins at the lateral surface of the nucleus. using an anatomically determined retinotopic map of the lgnd, the positions of all labelled cells near the lateral surface were conv ... | 1977 | 334561 |
| development of an enzyme-linked receptor assay (era) for hcg. | an enzyme-linked receptor assay (era) for hcg was developed using horseradish peroxidase. the 1,500 g pellets, interstitial cell fraction, and solubilized homogenate from the rat testis linked to tanned sheep red blood cells (srbc) were used as the binding fractions for hcg. in these era systems, the limit of detection for hcg was 30 iu/l (3-12 miu/tube), which was almost equal to that of radio receptor assay (rra). the era using srbc linked solubilized receptor fraction showed the most satisfac ... | 1977 | 334520 |
| the blood-brain barrier to horseradish peroxidase under normal and experimental conditions. | this review paper deals with the transport of the protein tracer horseradish peroxidase across cerebral vessels under normal and various experimental conditions. electronmicroscopical investigations have revealed that, under normal conditions, a minor vesicular transfer of intravenously injected peroxidase occurs across the endothelium in segments of arterioles, capillaries and venules, especially in arterioles with a diameter about 15-30 mu. this normally occurring vesicular transport is suscep ... | 1977 | 333857 |
| a specific enzymeimmunoassay for progesterone in human plasma. | an enzymeimmunoassay for plasma progesterone was established using progesterone covalently linked to the enzyme, horseradish peroxidase, as the 'label'. separation of free and bound steroid was effected by sepharose-coupled antiprogesterone-11alpha-hemisuccinyl bovine serum albumin antiserum (sepharose-antisera). the enzymeimmunoassay satisfied the normal criteria of specificity, precision and accuracy. comparison of assay results obtained by radioimmunoassay (with and without thin-layer chromat ... | 1977 | 333666 |
| evaluation of some of the parameters of the enzyme-linked immunospecific assay. | in an attempt to standardize the procedure for the enzyme-linked immunospecific assay, several parameters were examined. it was determined that horseradish peroxidase was the enzyme of choice and that several brands of polystyrene tubes and plates could be used. the test was considerably shortened by omitting long incubation steps previously used for eliminating background fixation of conjugate. the reproducibility of the procedure proved to be excellent, but reactivity curves indicated that use ... | 1977 | 333043 |
| immune complex receptors on cell surfaces. iii. topography of macrophage receptors demonstrated by new scanning electron microscopic peroxidase marker. | receptors for immune complexes have been localized on rabbit alveolar macrophages with scanning electron microscopy by exposing the cells first to a soluble immune complex composed of horseradish peroxidase and antibody to horseradish peroxidase, and then incubating with a benzidine-containing substrate that yields crystalline reaction product. receptors were visualized by this means as sites of attachment of laminated slender crystals that were easily distinguished from macrophage surface struc ... | 1977 | 333019 |
| the localization of proteoglycans and glycoproteins in the hyaline cartilage. | antibodies to proteoglycan (pg) and glycoprotein of bovine nasal cartilage were conjugated with fluorescein isothiocyanate and with horseradish peroxidase. hyaluronidase digestion of cartilage tissue-specimens increased the intensity of immune reactions; pronase digestion or extraction with 4 m guanidinium chloride abolished the staining. in the intercellular matrix fine filaments beaded with small granules were seen forming an irregular network. the interstices of the network are filled with co ... | 1977 | 332674 |
| [retinal projections in the lamprey, lampetra fluviatilis (horseradish perioxidase transport method in the optic nerve)]. | after the injection of horseradish peroxidase into the eye of the lamprey l. fluviatilis, the anterograde transport of the enzyme was found in the optic tract itself reaching the optic tectum and via single fibers--the geniculate nucleus. the labeled fibers were also found in the axial optic tract terminating in the geniculate nucleus and in the nucleus of the posterior commissurae. retrograde transport and labeling of the tracer were observed in cells of the dorsal and central tegmentum. | 2006 | 331759 |
| electron microscopic observations of cell coat of childhood leukemic cells using concanavalin a-horseradish peroxidase method. | 1977 | 327742 | |
| ultrastructural immunocytochemical localisation of lysozyme in human bronchial glands. | an immunocytochemical unlabelled antibody method using rabbit antihuman lysozyme, antirabbit immunoglobulin, and soluble rabbit antihorseradish peroxidase/horseradish peroxidase complexes was used to study the fine structural distribution of lysozyme in human bronchial glands. none was identified in mucous cells but there was heavy staining of the serous cell granules. the serous cell granules were not stained uniformly, suggesting the presence of other secretory products but lysozyme secretion ... | 1977 | 325682 |
| solid-phase enzyme immunoassay for hepatitis b surface antigen. | a solid-phase enzyme immunoassay is described for measuring hepatitis b surface antigen in human serum or plasma. immunologically purified antibody labeled with horseradish peroxidase was used as the indicator. in the assay system, antibody-coated controlled-pore glass is used as a solid support and there are three sequential incubations, totaling 2 h, at room temperature. results for serially diluted positive and reference sera compare favorably to radioimmunoassay in sensitivity and specifici ... | 1977 | 322892 |
| intimal healing. the pattern of reendothelialization and intimal thickening. | studies were undertaken to investigate further the basis of intimal proliferation and the identity of the surface lining cell in rabbit aortas subjected to extensive deendothelialization. endothelial cells were selectively removed by passage of an inflated balloon catheter through the arterial lumen. the healing response was evaluated at intervals up to 36 weeks by several techniques: 1) permeability to evans blue, 2) reappearance of endothelial cells as indicated by the specific marker, adsorbe ... | 1977 | 322503 |
| an ultrastructure study on antibody production of the lymph nodes of rats with special reference to the role of germinal centers. | antibody-containing cells in lymph nodes, expecially in germinal centers, and in thoracic duct of rats sensitized by injection of horseradish peroxidase were studied with the immunoperoxidase method. in the primary response the large germinal center cells began to produce antibody on the 9th day and predominated thereafter in germinal centers ,added by intercellular antibody deposition in its late stage. the "ordinary" medium-sized germinal center cells were a minor component. in the secondary r ... | 1977 | 322442 |
| the functional capacity of guinea pig megakaryocytes. ii. the uptake of particles and macromolecules and the effect of rabbit antiguinea pig platelet antiserum. | the capacity of megakaryocytes to take up particles and macromolecules was tested by exposing them to homologous erythrocytes, latex particles, micrococcus lysodeikticus, and horseradish peroxidase. uptake of particles 1.1 to 7 mum. in size was extensive under the experimental conditions employed. after uptake of each of the three particles the addition of tannic acid and calcium to the fixative produced electron-dense deposits within the vacuole surrounding the particle; therefore, the vacuole ... | 1977 | 320386 |
| caudate afferents from the retrorubral nucleus and other midbrain areas in the cat. | in order to clarify which midbrain areas project to the caudate nucleus (cd), horseradish peroxidase (hrp) was deposited in the cd of 13 cats. midbrain sections were reacted with diaminobenzidine and sometimes with cobaltous chloride to intensify the reaction, and then examined for retrograde labelling. afferents to the cd were found arising from the following ipsilateral midbrain areas: pars compacta, pars reticulata, and pars lateralis of the substantia nigra (sn), ventral tegmental area of ts ... | 1978 | 318187 |
| retrograde transport of horseradish peroxidase in sensory and adrenergic neurons following injection into the anterior eye chamber. | horseradish peroxidase (hrp) was injected into the anterior eye chamber of rats and mice and frozen sections from both superior cervical and trigeminal ganglia were incubated to demonstrate neurons accumulating the tracer by retrograde axonal transport. labelled cells were observed only in ganglia ipsilateral to the hrp injection. within the trigeminal ganglion, peroxidase-containing neurons were restricted to the medial ophthalmic area, whereas labelled cells in the superior cervical ganglion w ... | 1979 | 317295 |
| rapid stimulation of pinocytosis in human carcinoma cells a-431 by epidermal growth factor. | horseradish peroxidase (hrp) uptake was used to measure fluid-phase pinocytosis in monolayers of human epithelioid carcinoma cells (a-431). histochemistry confirmed that cell-associated hrp was restricted to intracellular vesicles. biochemical methods showed that hrp uptake in control cultures was directly proportional to the duration of exposure. the addition of low concentrations of epidermal growth factor (egf) to the incubation media produced a 10-fold increase in the initial rate of pinocyt ... | 1979 | 315944 |