Publications

the discrete anatomical localization of vagal aortic afferents within a catecholamine-containing cell group in the nucleus solitarius.	the centripetal transport of horseradish peroxidase (hrp) was used to visualize the central projections of afferent fibers of the aortic arch nerve to the nucleus solitarius in pigeons. our results indicate that aortic nerve afferents have a restricted distribution within one subdivision of the nucleus solitarius, designated the subnucleus sulcalis dorsalis. histofluorescence studies revealed that the aortic nerve projection area is extremely rich in catecholamine-containing neurons.	1979	466439
the effect of hyperglycemia on brain capillary permeability in the lizard, anolis carolinensis. a freeze-fracture analysis of blood-brain barrier pathology.	the anatomical basis of the blood-brain barrier in the american chameleon, anolis carolinensis, is the system of tight intercellular junctions that occurs between apposed endothelial cells of brain capillaries. under normal physiological conditions, capillaries in the brain cortex of these animals remain sealed by interendothelial zonulae occludentes and, consequently, escape of exogenous tracer proteins such as horseradish peroxidase (hrp) into the extracellular compartment of the central nervo ...	1979	466426
gaba-accumulating neurons in the nucleus raphe dorsalis and periaqueductal gray in the rat: a biochemical and radioautographic study.	the possibility of a gabaergic innervation of the nucleus raphe dorsalis (nrd) has been investigated by using the following approaches: (i) the identification of the principal neuronal groups afferent to the ndr by using horseradish peroxidase retrograde transport, (ii) the determination of glutamate decarboxylase activity (gad) in the nrd after lesioning these groups or their putative pathways, and (iii) the radioautographic identification of terminals axons and nerve cells accumulating intrave ...	1979	466412
localization of lumbar epaxial motoneurons in the rat.	in the rat, lateral longissimus and the lumbar transverso-spinalis muscles are lumbar trunk muscles, and participate in the lordosis reflex (female sexual posturing). medial longissimus, the remaining major lumbar epaxial muscle, is a muscle of the proximal tail-tailbase. to allow an analysis of the motor control of lordosis, motoneurons for these muscles must be localized. horseradish peroxidase (hrp) was injected into lumbar epaxial muscles of female rats. following injection into medial longi ...	1979	466406
the laminar and size distribution of commissural efferent neurons in the cat visual cortex.	the laminar and size distribution of commissural efferent neurons were studied in the cat visual cortex including area 17, 18, 19 and the lateral suprasylvian cortex (the lateral wall of the middle suprasylvian gyrus) by means of retrograde transport of horseradish peroxidase. single or multiple injections of the enzyme were made unilaterally along the medial part of the lateral gyrus (the adjoining parts of area 17 and 18) or along the lateral wall of the middle suprasylvian gyrus. in the contr ...	1979	464747
the morphopathologic substrates of concussion?	neuronal inundation with i.v. infused horseradish peroxidase was studied following concussive brain injury by means of both light and electron microscopy. in animals sustaining mechanical brain injury of insufficient intensity as to elicit either microscopic intraparenchymal hemorrhage or other neuropathological change, yet of sufficient intensity as to provoke a physiological concussive response, vascular peroxidase exudation concomitant with neuronal peroxidase inundation occurred throughout t ...	1979	463502
early influx of horseradish peroxidase into axons of the hypoglossal nerve during wallerian degeneration.	horseradish peroxidase (hrp) was applied around mouse hypoglossal nerves which were damaged by a crush or a ligature. hrp was then visualized distal to the lesions by light- and electron microscopic histochemistry. at the injury the enzyme entered axons and could also be detected several millimetres down in the distal segment. by 24 h reaction product (r.p.) was either diffusely distributed in the axoplasm or present in various vesicular organelles. our results indicate that there is a rapid inf ...	1979	460736
projections to the ventral surface of the cat brainstem demonstrated by horseradish peroxidase.	topical application of horseradish peroxidase (hrp) to the ventral surface of the medulla oblongata revealed various cells with axons terminating near this region. fibers traced from the ventral surface originated in the nucleus of the tractus solitarius and the dorsal nucleus of the vagus, both near the dorsal surface of the medulla. projections from the cochlear nucleus were also observed. using short hrp contact time, fibers were shown which terminate at, or near, the ventral surface. the pos ...	1979	460710
the comparative effectiveness of the "brown and blue reactions" for tracing neuronal processes of cells injected intracellularly with horseradish peroxidase.	the comparative advantages and disadvantages of diaminobenzidine (dab) and benzidine di--hcl (bdhc) procedures for tracing processes of cells injected intracellularly with horseradish peroxidase (hrp) have been studied. the dab reaction gives a good definition of membranes and appears more suitable for visualizing the fine structure of dendrites. the bdhc procedure, being more sensitive, induces crystal formation and heavily filled processes appear frosted. this last procedure is, however, much ...	1979	460707
the site of origin of cardiac preganglionic fibers of the vagus nerve: an hrp study in the cat.	after injection of horseradish peroxidase (hrp) into the right cardiac branches of the vagus nerve in the cat, the majority of hrp-labeled neurons were located ipsilaterally in the reticular formation ventrolateral to the nucleus ambiguus. additionally, hrp-labeled neurons were also observed within the nucleus ambiguus (am) and the dorsal motor nucleus of the vagus nerve (dm).	1979	460701
enhancing the anterograde movement of hrp to label sparse neuronal projections.	by combining techniques designed to increase the amount of horseradish peroxidase (hrp) available for transport and to improve the sensitivity of the reaction for detecting hrp, we have produced an anterograde hrp technique suitable for labeling sparse efferent projections. the efficacy of this light, microscopic technique was demonstrated by lebeling the hippocampal commissural and crossed temporo-ammonic projections to the dentate gyrus of the rat.	1979	460700
topographical representation in rabbit cerebellar flocculus for various afferent inputs from the brainstem investigated by means of retrograde axonal transport of horseradish peroxidase.	a small amount of horseradish peroxidase (hrp) was injected into several small areas of the flocculus and adjacent ventral paraflocculus of albino rabbits. labeled cells were surveyed through the inferior olive, vestibular nuclear complex, nucleus reticularis tegmenti pontis, pontine nucleus and abducens nucleus. distribution of labeled neurons in these structures varied depending on the location of injection sites, indicating the existence of a fine topographical organization for afferent input ...	1979	460699
the precise origin of the tectospinal pathway in three common laboratory animals: a study using the horseradish peroxidase method.	the horseradish peroxidase tracing method has been used to study the cells of origin of the tectospinal projections in the opossum, the tree shrew, and the cat. the present data show that only those collicular neurons which occupy the deep (ventral to the stratum opticum) tectal laminae send axons to the cervical spinal cord. in particular, layer iv contains the greatest number of spinal projecting neurons. our results also reveal that while only the large sized collicular neurons project upon t ...	1979	460682
cortical afferents to the prefrontal cortex of the cat: a study with the horseradish peroxidase technique.	following horseradish peroxidase (hrp) injections into different areas within the prefrontal cortex (pfc) of the cat, labeled neurons were found in the cingulate and insular cortex. these results demonstrate that the cat's prefrontal cortex is reached directly from these cortical regions, and that the observed cortical projections are similar to those detected in the monkey's prefrontal cortex.	1979	460681
[demonstration of the sources of several descending forebrain systems using the horseradish peroxidase retrograde axonal transport technic].	cells of cortical and diencephalic fibres descending to the mesencephalic and bulbar reticular formation and to the dorsal column nuclei were identified. according to findings neurons sending fibres to the mesencephalic reticular formation are located in orbitofrontal pole and in area 6 of the pericruciate cortex, mainly ipsilaterally. neurons which distribute fibres to the bulbar reticular formation are concentrated mainly in area 6 and 4 of the both hemispheres, while those distributing fibres ...	1979	460493
uptake and transport of horseradish peroxidase by lymphoepithelium of the bronchus-associated lymphoid tissue in normal and bacillus calmette-guérin-immunized and challenged rabbits.		1979	459427
latent lymphokines: isolation of guinea pig latent lymphocyte-derived chemotactic factor for monocytes. its activation by trypsin and a soluble factor from macrophages.	guinea pig lymphocytes when depleted of macrophages and stimulated by the t cell mitogen phytohemagglutinin produce a latent form of lymphocyte-derived chemotactic factor for monocytes (ldcf-m). latent ldcf-m is also produced when immune lymphocytes are stimulated in vitro with specific antigen, horseradish peroxidase. latent ldcf-m from both sources can be activated and converted to "classical" ldcf-m by trypsin and by a soluble factor obtained from sonicated macrophages. these observations sug ...	1979	458146
localization of vagal cardioinhibitory preganglionic neurons with rat brain stem.	in chloralose-urethane anesthetized spinal rats, electrical stimulation of systematically chosen points over the entire caudal brain stem area was carried out to explore the site(s) responsible for vagally mediated bradycardia. a dorsomedial locus including the nucleus dorsalis and the adjacent structures, the nucleus tractus solitarius, the nucleus commissuralis and the area postrema, and a ventrolateral locus around the nucleus ambiguus were found to elicit bradycardia with low threshold and h ...	1979	457932
quantitative studies of retinal ganglion cells in a turtle, pseudemys scripta elegans. i. number and distribution of ganglion cells.	multiple pathways for the transmission of visual information from retina to brain have been described in reptiles, but little is known about their functional organization. these parallel channels begin at the retina, and we have therefore begun to study the functional organization of retinal ganglion cells in the turtle, pseudemys scripta elegans. this paper describes the numbers and distribution of cells in the ganglion cell layer. to develop criteria for the identification of ganglion cells, w ...	1979	457929
effect of concanavalin a dose, unbound concanavalin a, temperature, ca2+ and mg2+, and vinblastine on capping of concanavalin a receptors of human peripheral blood lymphocytes.	the capping of concanavalin a (con a) receptors induced by con a was studied using human peripheral blood lymphocytes. the effects of con a dose (5--100 micrograms/ml), pretreatment at 4 degrees c, unbound con a, extracellular ca2+ and mg2+ and vinblastine were evaluated using con a-horseradish peroxidase and electron microscopy. lymphocytes incubated with con a at 4 degrees c and fixed with glutaraldehyde exhibited con a-horseradish peroxidase around the entire cell periphery. after raising the ...	1979	457812
endocytosis of cholera toxin in gerl-like structures of murine neuroblastoma cells pretreated with gm1 ganglioside. cholera toxin internalization into neuroblastoma gerl.	cholera toxin (ct), covalently attached to horseradish peroxidase (hrp), is a specific cytochemical marker for gm1 ganglioside (gm1) and retains the ability of the native toxin to raise levels of cyclic amp in avian erythrocytes. using a cytochemical stain for hrp, we found that 9% of control cultured murine neuroblastoma cells bound cholera toxin-horseradish peroxidase conjugates (ct-hrp) on their surfaces after incubations for 1 h at 4 degrees c. exogenous gm1, the natural receptor of ct, beco ...	1979	457774
morphological studies of stimulated adrenergic axon varicosities in the mouse vas deferens.	the postganglionic axons of sympathetic neurons innervating the mouse vas deferens were stimulated transmurally in vitro by passing square pulses between two platinum electrodes. the ultrastructural appearance of the adrenergic nerve terminals was compared to samples fixed immediately after 30 min of stimulation and in samples allowed to recover for 2 h before fixation. the contralateral vasa deferentia served as controls, and these were incubated in krebs solution for the same period as stimula ...	1979	457747
horseradish peroxidase as a label of injured cells.	the present study is concerned with artifacts likely to occur in a horseradish peroxidase exclusion test. incubation of murine peritoneal macrophages and lymphocytes with the peroxidase showed a close relationship between the number of living cells and the percentage of cells excluding the tracer. the penetration of the cytoplasm by horseradish peroxidase is attributed to an increase in the permeability of the cell membrane during the incubation (ranging from 10 to 120 min). it was not increased ...	1979	457441
permeability of retinal capillaries in rats with inherited retinal degeneration.	the permeability of retinal capillaries in rcs rats with inherited retinal degeneration was investigated with horseradish peroxidase used as a tracer. five-week-old rats showed typical degeneration of photoreceptor cells and accumulation of outer segment debris, but retinal capillaries were not permeable to peroxidase. at 10 weeks of age, capillaries in the inner retina appeared normal, but many in the outer retina were leaky. peroxidase activity in these latter vessels was demonstrable in the b ...	1979	457359
morphological evidence for axonal sprouting of cerebellothalamic neurons in kittens after neonatal hemicerebellectomy.	changes in cerebellothalamic projections in kittens after neonatal hemicerebellectomy were studied by the retrograde and anterograde horseradish peroxidase (hrp)-tract-tracing methods. the number of cerebellar nuclear neurons labeled retrogradely with hrp injected into the ipsilateral va-vl complex of the thalamus was much more numerous in neonatally hemicerebellectomized kittens than in intact kittens. presumed terminals of ipsilateral cerebellothalamic fibers labeled anterogradely with hrp inj ...	1979	456456
the projection of the temporal retina in rats, studied by retrograde transport of horseradish peroxidase.	horseradish peroxidase (hrp) was injected unilaterally into the lateral geniculate nucleus or tectum, or both, in 26 hooded rats in order to mark the exact extent of the retina from which uncrossed optic axons arise. this region occupied about a quarter of the retina, in the temporal periphery, following thalamic injections, but a much smaller region following tectal injections. by comparing the proportions of hrp positive neurones in nasal and temporal retinae of both eyes it was shown that: (1 ...	1979	456453
cat phrenic nucleus architecture as revealed by horseradish peroxidase mapping.	cat phrenic motoneurons, labeled by intradiaphragmatic injection of horseradish peroxidase, formed a tight cluster in the most ventral portion of the ventral horn in lamina ix of the lower cervical cord. cell counts were symmetrically distributed for 17 to 21 mm along the longitudinal axis of the cord with a unimodal peak at the junction of segments c5 and c6. the phrenic nucleus was bilaterally organized on either side of the cord with anatomical symmetry and in no case was there evidence for t ...	1979	456448
efferent projections of the parabigeminal nucleus in rats: a horseradish peroxidase (hrp) study.	the parabigeminal nucleus of the rat was made up of the dorsal, middle and ventral subgroups. when hrp was introduced into the superficial layer of the superior colliculus, labeled neurons were observed in the ipsilateral dorsal and ventral subgroups and in the contralateral middle subgroup. it was demonstrated that neurons of the dorsal and ventral subgroups sent fibers to the ipsilateral superior colliculus, and that those of the middle subgroup sent them to the anterior half of the contralate ...	1979	455073
axon trajectories of renshaw cells in the lumbar spinal cord of the cat, as reconstructed after intracellular staining with horseradish peroxidase.		1979	455060
[epithelial permeability study of the bronchi in chronic bronchitis using an electron-dense tag].	an electron dense tracer, horseradish peroxidase (hrp), treated with dab-h2o2 for electron histochemical demonstration of peroxidase was used in an effort to find out whether or not the human bronchial epithelium was permeable to some macromolecules in chronic bronchitis. the biopsy specimens obtained from 5 patients at bronchoscopy and cultured in a medium with 2% hrp for 1-6 hours showed the reaction product to be distributed within intracellular spaces of the epithelium as well as in some ves ...	1979	454230
bactericidal effect of cysteine exposed to atmospheric oxygen.	peptostreptococcus anaerobius vpi 4330-1 was exposed to atmospheric oxygen in a dilution bland (0.2% gelatin, salts, resazurin) solution. the organisms were rapidly killed when the solution contained cysteine. the organisms were effectively protected by catalase and horseradish peroxidase as well as by the metal ion-chelating agents 8-hydroxyquinoline and 2,2'-bipyridine. superoxide dismutase increased the rate of killing of the organisms, whereas singlet oxygen quenchers and scavengers of hydro ...	1979	453819
a study of the perineurial diffusion barrier of a peripheral ganglion.	the perineurial diffusion barrier to horseradish peroxidase (hrp) and ferritin was investigated in superior cervical ganglia of rats and mice. the ganglion was surrounded by a delicate epineurium and 2-5 perineurial lamellae joined by zonulae occludentes and desmosomes. following local application of tracers the animals were killed after 5, 30, and 60 min and the distribution of hrp and ferritin was studied by light and electron microscopy. the inner layers of the ganglionic perineurium prevente ...	1979	452854
quantitative estimate of pinocytosis in experimental acute hypertension.	cerebral cortical arterioles in focal neocortical areas develop increased permeability to plasma proteins and protein tracers in experimental hypertensive encephalopathy. the mechanism underlying this increased permeability has been the subject of several studies. in our previous studies of angiotensin-induced acute hypertension, pinocytosis appeared to be the principal mechanism for the increased blood-brain barrier (bbb) permeability observed. in the present study pinocytotic activity was asse ...	1979	452852
structure of physiologically identified x and y cells in the cat's lateral geniculate nucleus.	horseradish peroxidase injected into 18 single, physiologically identified geniculate x and y cells permitted a detailed morphological correlate to be determined for the physiological properties of each neuron. class 1 morphological characteristics were associated with y cells, class 3 with x cells, and class 2 structural traits were seen in both physiological types.	1979	451559
morphological features of functionally defined neurons in the marginal zone and substantia gelatinosa of the spinal dorsal horn.	functional characteristics of spinal neurons located in the marginal zone (lamina i) and substantia gelatinosa (lamina ii) were compared to their structural features by intrcellularly staining the source of unitary potentials with horseradish peroxidase (hrp) in unanesthetized, spinal cats. the responses of postsynaptic units to graded electrical volleys in intact dorsal roots and to physiological stimulation revealed that the peripheral excitatory input to neurons of the region is dominated by ...	1979	447881
reexamination of the dorsal root projection to the spinal dorsal horn including observations on the differential termination of coarse and fine fibers.	primary afferent fibers in the lumbar, sacral, and caudal spinal segments of several mammals (rat, cat, monkey) were stained by applying horseradish peroxidase to the proximal part of cut dorsal rootlets and reacting the tissue histochemically after several hours of survival. the stained fibers' pattern of termination in the dorsal horn was similar in all three species, with many bouton-like enlargements in the ipsilateral marginal zone, substantia gelatinosa, and nucleus proprius, as well as a ...	1979	447880
an hrp and autoradiographic study of the projection from the cerebellar cortex to the nucleus interpositus anterior and nucleus interpositus posterior of the cat.	the recently developed anatomical techniques of retrograde transport of the enzyme horseradish peroxidase (hrp), anterograde transport of tritiated amino acid, and intracellular injections of hrp were used to study the organization of the corticonuclear projection to the nucleus interpositus anterior (nia) and the nucleus interpositus posterior (nip) of the cat. injections of hrp into the nia and the nip revealed that the major areas of the cortex which provided afferents to these two nuclei wer ...	1979	447879
abnormal retino-geniculate and geniculo-cortical pathways in several genetically distinct color phases of the mink (mustela vison).	several genetically distinct color phases of mink, which all show an abnormal reduction of pigment in the retinal pigment epithelium and which also show abnormalities of the retinofugal pathways, have been studied. autoradiographic methods have been used to demonstrate the retino-geniculate pathways, and retrograde degeneration or the retrograde transport of horseradish peroxidase has been used for the geniculo-cortical pathways. the retino-geniculate abnormality is mild in some of the color pha ...	1979	447875
prefrontal cortex of the cat: evidence for an additional area.	direct projections from the mediodorsal nucleus of the thalamus to ventral parts of the insular region of the cat's cortex were demonstrated by using the horseradish peroxidase technique.	1979	446633
quantitative histological study of spinal afferent innervation on the ventral surface of the cat stomach by horseradish peroxidase (hrp) method.	retrograde axonal transport of horseradish peroxidase (hrp) was applied to the ventral surface of the cat stomach. we investigated the number, size and distribution of hrp-positive cells in spinal ganglia. the unexpected finding was the wide distribution of these cells from t3 down to l3. this would result in a diffuse pattern of referred pain.	1979	446627
intercellular junctions and the development of the blood-brain barrier in manduca sexta.	in early embryonic development of the tobacco horn moth no blood-brain barrier is present, as shown by the unimpeded entry of exogenous tracers into the nervous system. however, later on, just before hatching, lanthanum and horseradish peroxidase (hrp) are unable to move inwardly beyond the level of the perineurium, which is the morphological site of the blood--brain barrier in the adult moth, as well as in other insects. freeze-fracture studies indicate that in the early embryo, 10 nm particles ...	1979	445142
chemical and enzymatic intermediates in the peroxidation of o-dianisidine by horseradish peroxidase. 2. evidence for a substrate radical--enzyme complex and its reaction with nucleophiles.	changes in the optical absorption spectrum of horseradish peroxidase, during the oxidation of o-dianisidine at ph 7.5, reveal an intermediate distinct from the previously described compounds i and ii. the rate of decay of this new complex appeared to be rate limiting for the catalytic cycle, in this ph range, since imidazole, which augments the catalytic reaction, also enhanced the rate of decay of this complex. nitrogenous compounds reportedly unable to ligate to hemes, such as 2-methylimidazol ...	1973	444459
inhibition of the discharge of endocytosed protein from phagosomes into lysosomes in hepatoma cells exposed to dimerized ribonuclease a.	the mechanism of the cytostatic action of dimerized ribonuclease a toward cultured hepatoma cells was investigated. a decrease in mitotic index, modifications of adsorptive properties of the pericellular membrane and inhibition of the degradation of two different proteins taken up by endocytosis are the first cell functions to be affected by the dimer. this effect on protein digestion is not due to an inhibition of proteolytic enzymes. the intracellular localization of exogenous protein and of r ...	1979	444221
[horseradish peroxidase labeled neurons of the ventrobasal complex of the thalamus projecting to the somatosensory zone i of the cat cerebral cortex].	the morphology and topography of the neurons in the ventro-basal complex (vb) projecting to the somatic sensory cortex (si) were studied by the method of the retrograde axonic transport of horseradish peroxidase (hrp). different types of hrp-labelled cells were found in this structure. the concentration of labelled cells was the greatest in the central part of the vb. the most peculiar feature in the distribution of hrp-positive neurons was their organization in two large populations in the dors ...	1979	440485
early and late mechanisms of increased vascular permeability following experimental cerebral infarction.	cerebral infarction was produced in rats by a combination of transient unilateral common carotid artery occlusion and systemic hypoxia. horseradish peroxidase (hrp) and evans blue were given 5 minutes prior to sacrifice to assess the integrity of the blood-brain barrier (bbb) at 1 minute, 30 minutes, and 2 hours following the ischemic insult. there was immediate permeability to hrp in the early (1 minute and 30 minutes) post-ischemic period, whereas, evans blue was not seen until the late (1.5 t ...	1979	438863
histochemical identification of acetylcholinesterase in dopaminergic nigrostriatal neurons.	by combining histochemical procedures, evidence was obtained in the rat that acetylcholinesterase (ache) is contained within perikarya of dopaminergic substantia nigra cells which project to the caudoputamen. nigrostriatal neurons were identified by retrograde transport of horseradish peroxidase (hrp). simultaneous incubation of substantia nigra sections for the presence of ache and hrp demonstrated both reaction products in the same cell bodies. the catecholaminergic nature of these cells was d ...	1979	438805
a microplate immunoenzyme assay for anti-influenza antibodies.	an enzyme-linked immunosorbent assay (elisa) using horseradish peroxidase is described for the detection and quantitation of anti-influenza virus antibodies. compared with complement fixation and hemagglutination inhibition tests, elisa is far superior with respect to sensitivity and reliability. non-specific viral inhibitors present in sera do not affect the titer in elisa. its sensitivity, close to that of radioimmunoassay permits detection of small amounts of antibodies in pulmonary secretion ...	1979	438513
opening of tight junctions in cerebral endothelium. ii. effect of pressure-pulse induced acute arterial hypertension.	acute arterial hypertension was produced in male wistar rats by pressure pulse through the right internal carotid artery. the pressure pulse was induced by infusion of physiological saline as a bolus, at a rate of 0.63 ml per second by syringe pump. evans blue (eb) was used to visualize the areas of blood-brain barrier opening. intravenously injected horseradish peroxidase (hrp) was used to study the ultrastructural basis of permeability changes in cerebral endothelium. eb outlined circumscribed ...	1979	438371
opening of tight junctions in cerebral endothelium. i. effect of hyperosmolar mannitol infused through the internal carotid artery.	infusion of 1.8 m mannitol solution into the internal carotid artery of wistar rats allows horseradish peroxidase (hrp) to cross cerebral vascular endothelium via intercellular pathways. this was made evident by density gradients of hrp observed in consecutive tight junctional compartments following in vivo administration, and by the passage of colloidal lanthanum through junctional membrane appositions of glutaraldehyde fixed cerebral endothelium. three hours after mannitol infusion, small non- ...	1979	438370
the morphological and physiological properties of a regenerating synapse in the c.n.s. of the leech.	regeneration of an electrical synapse between particular interneurons in the medicinal leech was traced physiologically and morphologically using intracellular recording the horseradish peroxidase (hrp) injection. the synapse between s-cell interneurons lies in the connective midway between segmental ganglia, so crushing near one ganglion severs only one s-cell's axon. the severed distal stump remains connected to the adjacent uninjured s-cell and continues for weeks to conduct impulses. the inj ...	1979	438368
glomerular permeability of macromolecules. effect of molecular configuration on the fractional clearance of uncharged dextran and neutral horseradish peroxidase in the rat.	molecular parameters other than size and charge are likely to influence the filtration of macromolecules across the glomerular filter. we have studies, therefore, the glomerular permeability of macromolecules with widely different molecular configuration such as horseradish peroxidase, a plant glycoprotein with an isoelectric point in the physiologic ph range, and dextran, an uncharged sugar polymer of d-glucopyranose. simultaneous fractional clearances were determined for both test macromolecul ...	1979	438331
the uptake of horseradish peroxidase by damaged autonomic nerves in vitro.	the uptake of horseradish peroxidase by damaged autonomic nerves was studied in vitro. large amounts of the enzyme penetrated the axons in a diffuse (nonvesicular) form. this occurred within 90 minutes of application of the enzyme. twenty four hours after application of the enzyme diffuse peroxidase was still present in the axoplasm, but it was 'diluted' as a result of great axonal swelling. pinocytotic uptake of the enzyme was observed both 90 minutes and 24 hours after application of the enzym ...	1979	438097
uptake of exogenous protein by supraependymal cells of the feline area postrema.	supraependymal cells occurring on the surface of the feline area postrema were examined for phagocytic ability. it was shown that they could ingest exogenous horseradish peroxidase that was experimentally introduced into the brain ventricular system. the cells thus bear functional as well as ultrastructural attributes of macrophages, similar to those found in the third ventricle and subarachnoid space.	1979	437052
development of spinal motoneuron innervation of the upper limb muscle in the rat.	horseradish peroxidase was injected into the biceps brachii muscle of rats at various stages of development, from 10 days to 50 weeks. the retrogradely labeled neurons were found in the ipsilateral ventro-lateral column of the cervical cord, c4--c8, of all stages studied, but the number of labeled neurons decreased according to exponential curve as the age advanced. a striking finding was that the contralateral ventral horn cells were also labeled in the 10- and 14-day-old rats.	1979	436997
the fine structure of neurones and cellular relationships in the abducens nucleus in the cat.	light and electron microscope studies of the abducens nucleus in the cat have disclosed two populations of neurones: large neurones 25 to 75 micrometer in diameter and small neurones 10 to 25 micrometer in diameter. characteristic of the large neurones are a highly developed granular endoplasmic reticulum and a large number of axo-somatic synapses. the small neurones have a poorly developed granular endoplasmic reticulum and comparative by few axo-somatic synapses. injection of horseradish perox ...	1979	436996
thalamic projections from the lateral reticular nucleus in the opossum as determined by horseradish peroxidase histochemistry.		1979	435983
the role of protein and porphyrin in the reactivity of horseradish peroxidase toward hydrogen donors.		1979	435299
cerebellar afferents from the nucleus of the solitary tract.	the cerebellar afferents from the nucleus of the solitary tract were studied in cat by means of retrograde axonal transport of horseradish peroxidase. labelled cells occurred bilaterally in the nucleus of the solitary tract following injections in various folia of the cerebellar vermis and in the flocculus (the positive cases are shown in fig. 1a). injections in the anterior lobe vermis labelled cells in the caudal part of the nucleus, injections in the posterior vermis labelled cells in the ros ...	1979	431884
direct projection of catecholamine (presumably dopamine)-containing neurons from hypothalamus to spinal cord.	a procedure for combining catecholamine (ca) and horseradish peroxidase (hrp) histochemistry was used to demonstrate that some of the catecholamine-containing cells in the rabbit dorsal hypothalamus project directly to the thoracic spinal cord. these cells, which are probably dopaminergic, may be involved in the integration of pituitary hormonal secretion and peripheral autonomic nervous regulation.	1979	431883
identification of cerebellar corticovestibular neurons retrogradely labeled with horseradish peroxidase.		1979	431827
patterns of subcortico-cortical projections as revealed by somatopetal horseradish peroxidase tracing.		1979	431824
endocytosis in glial cells (pituicytes) of the rat neurohypophysis demonstrated by incorporation of horseradish peroxidase.		1979	431821
submandibular glandular permeability to blood-borne horseradish peroxidase in dogs, before and after obstructing secretion [proceedings].		1979	430395
localization of neurons in the rat superior cervical ganglion that project into different postganglionic trunks.	horseradish peroxidase (hrp) was used to determine whether neurons in the rat superior cervical ganglion (scg) are localized in regions of the ganglion as a function of the postganglionic trunk they utilize. in separate experiments, each of the two major postganglionic trunks was cut 1-3 mm from the scg and solid hrp was applied to the cut end proximal to the ganglion. the results demonstrated that the cell bodies of neurons whose axons project out the internal carotid nerve (icn) were located p ...	1979	429622
the lateral cervical nucleus in the cat: anatomic organization of cervicothalamic neurons.	the morphology of the lateral cervical nucleus (lcn) and the organization of the cervicothalamic projection neurons were studied in cats which had received thalamic injections of horseradish peroxidase (hrp). the boundaries of the lcn were defined following very large thalamic (hrp injections. roughly 92-97% of lcn cells project contralaterally to thalamus; an additional 1.5% project ipsillaterally. computer-assisted measurements of perikaryal areas demonstrated that there are two sizes of lcn c ...	1979	429620
sympathetic neurons in the cat spinal cord projecting to the stellate ganglion.	horseradish peroxidase (hrp) was injected into the stellate ganglia of anesthetized cats. after a 2-day survival time the spinal cord was processed for the hrp reaction to study the distribution of the preganglionic neurons which project to the stellate ganglia. hrp-labeled neurons in the cord were located exclusively on the side ipsilateral to the injected stellate ganglia and were concentrated in four distinct areas: (1) intermediolateral cell column (78.2%), (2) lateral funiculus (18.5%), (3) ...	1979	429616
mechanism of the chlorination reaction catalyzed by horseradish peroxidase with chlorite.		1979	429342
nuclear magnetic resonance studies of hemoproteins. binding of aromatic donor molecules to horseradish peroxidase.		1975	429320
morphologic study of the action of lymphoid tissue extracts in peroxidase arthritis.	by means of modelling arthritis by horseradish peroxidase it was possible to reproduce a process corresponding to immune synovitis. a considerable reduction of immunomorphological phenomena in joints, lymphoid tissue, heart, liver and kidneys was favoured by application of lymphoid tissue extracts. comparison between the extracts of spleen and lymph nodes showed a more pronounced effect of spleen extract.	1979	428522
localization of parabrachial nucleus neurons projecting to the thalamus or the amygdala in the cat using horseradish peroxidase.		1979	428513
physiological properties of stalked cells of the substantia gelatinosa intracellularly stained with horseradish peroxidase.		1979	427560
spinal axon collaterals of corticospinal neurons identified by intracellular injection of horseradish peroxidase.		1979	427559
the differential absorptive activity of epithelial cells of the rat epididymus before and after castration.	horseradish peroxidase introduced into the lumen of the rat epididymis was taken up by the columnar cells of the epithelium by five minutes and more so after longer periods. the apical cells and particularly the clear cells in the caput and cauda epididymis, respectively, showed significantly greater endocytotic activity than the principal cell in both locations. within 14 days after castration, however, such differences in absorptive activity among the various cell types were essentially obscur ...	1979	426301
distribution of surface coat material on nasal folds of mouse embryos as demonstrated by concanavalin a binding.	3h-concanavalin a and the concanavalin a-horseradish peroxidase staining technique were used to study the distribution of surface coat material on the epithelium of the nasal folds and nasal groove of mouse embryos. in stages shortly before and during epithelial fusion concanavalan a stained or labeled material was present at apical surfaces of epithelial cells of the nasal groove and nasal folds. silver grains, representing bound 3h-concanavalin a, were counted in defined areas of the nasal gro ...	1979	426293
the heterophagic granules of mast cells: dipeptidyl aminopeptidase ii activity and resistance to exocytosis.	dipeptidyl aminopeptidase ii (dap ii) was demonstrated cytochemically in rat peritoneal mast cells. the bright red reaction product in smears of peritoneal wash fluid tended to be localized in one to eight granules in the perinuclear region of the cell. this finding was confirmed at the electron microscopic level, where a small proportion of the granules, most often located near the nucleus, revealed electron opacity indicative of dap ii. focal areas in the nuclear envelope densified by dap ii r ...	1979	426034
endocytosis in chronically denervated mouse skeletal muscle. a biochemical and ultrastructural study with horseradish peroxidase.		1979	424075
commissural and intrinsic connections of the rat hippocampus.	the commissural and intrinsic connections of the hippocampus were studied using the fink-heimer method and the horseradish peroxidase (hrp) uptake technique. a conspicuous septo-termporal gradient was found of the density of the commissural projection that passes through the psalterium ventrale to the ammon's horn. the degeneration resulting from transection of the psalterium ventrale was most dense in the septal tip and decreased towards the temporal tip. the commissural and ipsilateral connect ...	1979	422759
projections from the spinal and the principal sensory nuclei of the trigeminal nerve to the cerebellar cortex in the cat, as studied by retrograde transport of horseradish peroxidase.	projections from the spinal (vsp) and the principle sensory (vp) nuclei of the trigeminal nerve to the cerebellar cortex were studied by means of retrograde transport of horseradish peroxidase in the cat. neurons projecting to the simple lobule and the dorsal part of the paramedian lobule (pmd) were located mainly in the dorsal part of the nucleus interpolaris (vi) and of the caudal one third of the nucleus oralis (vo) and in the rostralmost part of the vo. neurons projecting to the medial part ...	1979	422757
somatic neurons in the brainstem and neocortex projecting to the external nucleus of the inferior colliculus: an anatomical study in the opossum.	some brainstem and neocortical neurons projecting to the external nucleus of the inferior colliculus were identified by retrograde axonal transport of horseradish peroxidase (hrp). large pressure injections of hrp were used to define the population of cells in the dorsal column and trigeminal nuclei projecting to the entire inferior colliculus. subsequently, tiny injections of hrp were delivered electrophoretically to various parts of the external nucleus using micropipettes. in each case the el ...	1973	422756
the permeability of rat palatal mucosa maintained in organ culture.	the intercellular permeability barrier of neonatal rat palatal mucosa maintained in organ culture for periods up to 24 days was studied ultrastructurally using the tracers horseradish peroxidase and lanthanum nitrate. at all time intervals examined the limit of penetration of the tracers corresponded to the level at which the membrane-coating granules were being discharged. however, in the cultured mucosa, extrusion of granules occurred closer to the granular cell-keratin junction after 6 and 12 ...	1979	422477
anatomical and electrophysiological identification of motoneurones supplying the cat retractor bulbi muscle.	motoneurones innervating the retractor bulbi muscle in the cat have been identified by retrograde labelling with horseradish peroxidase, by intracellular recording and by intracellular staining with horseradish peroxidase. their somata are found in an accessory abducens nucleus, analogous to that described in some other species, which consists of a narrow column of cells situated in the lateral tegmental reticular field, above the superior olive and medial to the facial nerve. the column of cell ...	1979	421760
the pontine projection onto longitudinal zones of the paramedian lobule in the cat.	the distribution of labeled cells in the pontine nuclei was studied following microinjections of horseradish peroxidase into three longitudinal zones in the paramedian lobule in the cat. labeled cells were only found when the staining of the cerebellar cortex included the granular layer. the labeled cells were present contralateral to the injection site in the cases with the smallest injections, in the others there was a bilateral distribution with a contralateral preponderance. the labeled cell ...	1979	421749
the vestibular nuclei in the domestic hen (gallus domesticus).	stereotaxic injections of small quantities of horseradish peroxidase (hrp) are made in the vestibular complex in the hen and the labelled cells due to retrograde transport of the tracer enzyme are studied, especially in the vestibular complex but also in other parts of the brain stem. the superior vestibular nucleus sends commissural fibres to the superior, medial, and descending nuclei and to deiters' complex. the cell group a projects to the contralateral superior nucleus and to the deiter's c ...	1979	421748
projection from the vestibular nuclei to the inferior olive in the cat: an autoradiographic and horseradish peroxidase study.	injections of horseradish peroxidase (hrp) in the inferior olive of 13 cats resulted in the labeling of neurons in the medial (mvn) and descending vestibular nuclei (dvn) as well as in subgroups g, p.h., s.v., x and z. the contribution of groups p.h. and z. could not be definitively established. injections of triated leucine in the vestibular nuclei and adjacent reticular formation in 4 cats indicated that the previously identified vestibular nuclei and subgroups project bilaterally to the dorso ...	1979	421137
rapid preparation of peroxidase: anti-peroxidase complexes for immunocytochemical use.	soluble immune complexes of horseradish peroxidase and antibody to peroxidase (pap) have been widely used in the 'unlabelled antibody' method for the immunocytochemical detection of cellular antigens. this paper describes a simple and rapid method for preparation of these complexes by column chromatography of a mixture of the enzyme and the igg fraction of antiperoxidase antiserum on sephacryl s-200. the material eluting in the void column consists of stable soluble pap complexes, with a molar p ...	1978	418119
thalamic projections to s-i in macaque monkey.	the organization of thalamic input to functionally characterized zones in primary somatosensory cerebral cortex (s-i) of macaque monkeys (macaca mulatta) was investigated using the method of labelling by retrograde transport of horseradish peroxidase (hrp). it was found that the cell columns positioned at the posterior margin of the band of cortex representing a given body region receive thalamic input from a posterior level of the ventroposterior thalamic nucleus (vp), and that cell columns at ...	1978	418081
[electrophysiologic and anatomic demonstration of inhibitory commisural vestibular neurons in the tench (tinca tinca)].	horseradish peroxydase was injected intracellularly in some of the medullary neurons which, in fish, exhibit a passive hyperpolarizing potential after spinal cord stimulation (this indicates that such neurons both electrical and chemical inhibitions of the mauthner cells). with positive staining, commissural vestibular neurons were identified; their axons cross the midline and they establish connexions with the mauthner cell and with vestibular neurons of both sides. thus the existence of a cros ...	1978	417859
[cells secreting antibodies originate from cells secreting immunoglobulins without a detectable antibody function for the antigen injected].	cells secreting immunoglobulins without detectable antibody function arising after an injection of horseradish peroxidase were micromanipulated from the center of haemolytic plaques of sheep red blood cells coated with anti-ig antibodies. these cells were cultured individually for 48 hrs, with irradiated cells as feeder layer and in the presence of the immunogen and of lps. it was shown that after this time 22% of the immunoglobulin-secreting cells had generated antiperoxidase antibody-secreting ...	1977	417838
[organization of contralateral and bilateral projections of corticospinal tracts in cats].	in cats with a previous spinal lesion (c 6) saving only a lateral or ventral funiculus, a caudal horseradish peroxidase injection (c 8-th 1) reveals that some cortical zones (lateral sigmoïd gyrus, medial part of the posterieur sigmoïd gyrus, medial part of the dorsal bank of the cruciate sulcus) send spinal projections, only contralaterally, by way of the dorsolateral funiculus, whereas other cortical zones (anterior sigmoïd gyrus, middle part of the posterior sigmoïd gyrus, lateral parts of th ...	1977	417815
calcium-related properties of horseradish peroxidase.		1978	416823
fixation variables in horseradish peroxidase neurohistochemistry. i. the effect of fixation time and perfusion procedures upon enzyme activity.	in a series of neurohistochemical experiments the effect of aldehyde fixation upon the detection of horseradish peroxidase (hrp) was examined. these experiments demonstrated that: a) increments in fixation of as little as 1 hr significantly decreased the number of labeled neurons; 12-hr fixation abolished hrp activity in many neuronal populations and significantly reduced the apparent size of the injection site. b) this negative fixation effect was greatest where the hrp concentration was low (e ...	1978	413864
the intracerebellar nucleocortical projection in a primate.	experiments were performed to determine if a nucleocortical system, a projection from the cerebellar nuclei to the cerebellar cortex, was present in primates. both electrophysiological and neuroanatomical techniques were employed to investigate this question. it was shown that neurons within the dentate and interposed nuclei were antidromically activated by stimuli applied to the cerebellar cortex. in addition, cells in these nuclei were retrogradely labelled following injections of small amount ...	1977	413728
[morpho-histochemical characterization of the connections between the dorsal part of the lateral geniculate nucleus (lgn) and the brain stem of albino rats) (author's transl)].	after selective application of horseradish peroxidase (hrp) into the dorsal part of the lateral geniculate nucleus (lgn) of the albino rat, cells were labelled through the retrograde transport of the enzyme in the following nuclei of the brain stem: 1. ncl. raphes linearis, 2. nel, raphes centralis, 3. ncl. raphes dorsalis, 4. ncl. ventralis rostralis lemnisci lateralis, 5. ncl. coeruleus. using the glyoxylic acid method to demonstrate biogenic amines we could find serotonine in following areas ...	1977	413324
some claustro-cortical connections in the cat and baboon as studied by retrograde horseradish peroxidase transport.	1. the mammalian claustrum (cl) is a convergent multisensory structure of unknown function, and disputed ontogenetic origin. its cortical projections, hitherto unknown, have been studied in cat and baboon by means of the horseradish peroxidase (hrp) technique. hrp was injected into the gyrus proreus (frontal eye field) of cats, and separately into the frontal eye fields, visual areas, and motor-premotor areas of the baboon cortex. 2. differential retrograde transport to the cl was demonstrated, ...	1978	412882
fine structural and ultracytochemical studies on the lymphocytes in three types of genetic mucopolysaccharidoses.	lymphocytes from 6 patients with 3 types of genetic mucopolysaccharidoses (hurler's syndrome, hunter's syndrome and morquio's syndrome) contained numberous vacuoles in their cytoplasm. the size of the vacuoles ranged from approximately 300 nm to 750 nm. the percentage of the lymphocytes with vacuoles varied from 10% to 38%. the vacuoles showed acid phosphatase activity, which indicated their lysosommal nature. staining with dialyzed iron solution usually localized acid mucosubstance in the perip ...	1977	412306
the projections of the entopeduncular nucleus and globus pallidus in rat as demonstrated by autoradiography and horseradish peroxidase histochemistry.		1978	411809
[anatomical identification of the vestibulo-olivary and cerebello-olivary projections in the cat].	injections of horseradish peroxidase were made into different parts of the inferior olive in the cat. it is shown that the vestibular nucleus z and, to a lesser extent, the nucleus f send off fibres to the ipsilateral medial accessory olive. the ventral part of the nuclei inter-positus and dentatus send off fibres to the ventrolateral outgrowth of the opposite side.	1977	411581
limbic and sensory connections of the inferior parietal lobule (area pg) in the rhesus monkey: a study with a new method for horseradish peroxidase histochemistry.	the caudal part of the inferior parietal lobule (area pg) was injected with horseradish peroxidase (hrp) in 6 hemispheres of 5 rhesus monkeys. the retrograde transport of hrp resulted in the labeling of neurons in diverse cortical and subcortical areas. in cortex, labeled neurons were noted in prefrontal cortex (areas 8, 45, 46), in the banks of the intraparietal and superior temporal sulci, in medial parietal cortex, in cingulate cortex, in the retrosplenial area, in area tf and the caudal port ...	1977	411543
organization of the thalamo-cortical connexions to the frontal lobe in the rhesus monkey.	in 25 rhesus monkeys horseradish peroxidase was injected in different parts of the frontal cortex. the retrogradely labelled thalamic neurons formed longitudinal bands, some of which crossed the internal medullary lamina, and extended from one thalamic nucleus into another. on the basis of these findings the frontal cortex was subdivided into seven transverse cortical strips which receive afferents from seven longitudinal bands of thalamic neurons. the most rostral transverse strip receives affe ...	1977	410652
thalamic projections of the hippocampal formation: evidence for an alternate pathway involving the internal capsule.	experiments were performed using [3h]leucine radioautography and horseradish peroxidase histochemistry to determine the origin and distribution of the projection from the hippocampal formation to the thalamus in the rat. the results confirm recent findings that postcommissural fornix fibers which project to the anterior thalamus arise exclusively from the subicular cortex and not from hippocampal pyramidal cells. a second significant feature of this pathway is that it arises from cells situated ...	1977	410479