Publications

proceedings of the 3rd north sea conference on periodontology. maastricht, the netherlands, 16-19 may 1990.		2006	1679767
gold therapy in rheumatoid arthritis.		1993	1679184
restriction fragment length polymorphism analysis of vibrio cholerae strains associated with a cholera outbreak in hong kong.	we studied vibrio cholerae el tor isolates associated with an outbreak of cholera among vietnamese refugees interned in hong kong. the restriction fragment length polymorphism of the enterotoxin gene was used as an epidemiological marker. all outbreak strains were indistinguishable. they were distinct from strains isolated in hong kong prior to the outbreak.	1991	1676035
survival of classic cholera in bangladesh.	during the present cholera pandemic the el tor biotype of vibrio cholerae has completely displaced the classic biotype, except in bangladesh. we studied the distribution of these two biotypes in twenty-four rural districts during epidemics in 1988-89; there was clustering of the classic biotype in the southern region and of the el tor biotype in all other regions. these findings suggest that the southern coastal region is now (and may always have been) the habitat of classic cholera. the selecti ...	1991	1674016
difference between toxigenic vibrio cholerae o1 from south america and us gulf coast.		1991	1673518
cardiac electrophysiologic effects of adaprolol maleate, a new beta-blocker, in closed chest dogs.	adaprolol maleate (adaprolol) is a new beta-adrenergic receptor blocker. the cardiac electrophysiologic characteristics and the duration of action of adaprolol were studied in intact anesthetized dogs. the electrophysiologic parameters were evaluated before drug administration and 10 min after the administration of maintenance infusion of adaprolol (n = 5). the duration of action was measured during constant infusion of isoproterenol, giving the adaprolol as a single i.v. bolus in different conc ...	1991	1673212
cardiac electrophysiologic effects of adaprolol maleate, a new beta-blocker, in closed chest dogs.	adaprolol maleate (adaprolol) is a new beta-adrenergic receptor blocker. the cardiac electrophysiologic characteristics and the duration of action of adaprolol were studied in intact anesthetized dogs. the electrophysiologic parameters were evaluated before drug administration and 10 min after the administration of maintenance infusion of adaprolol (n = 5). the duration of action was measured during constant infusion of isoproterenol, giving the adaprolol as a single i.v. bolus in different conc ...	1991	1673212
biotype as determinant of natural immunising effect of cholera.	to test the hypothesis that clinical vibrio cholerae o1 infections protect against recurrent cholera, treated cholera episodes in a rural bangladesh population of 188,153 people who were followed between 1985 and 1988 were analysed. of the 2214 people with initial episodes of cholera, 7 had a second episode. the incidence of cholera was 61% lower in subjects who had had an earlier episode than in those without such an episode. whereas initial episodes of classical cholera were associated with co ...	1991	1672971
non-o1 vibrio cholerae gastroenteritis and bacteraemia.		1991	1672411
vibrio cholerae o1 el tor inaba.		1991	1669889
[immunocytochemical detection of creatine kinase isoenzymes (ck bb) in human cerebral cortex].	the localization of creatine phosphokinase-bb (cpk-bb) in the neocortex of persons without mental pathology, who died of cardiovascular diseases (n-15) was investigated immunocytochemically. it has been found that there are several differences in the visualization of cpk-bb in neurons and astrocytes under various conditions of autopsy material treatment. the data obtained may attest to the existence of the immunochemically different forms of cpk-bb in the human brain. the appropriate conditions ...	1991	1665650
regulation of the mouse mammary tumor virus (mmtv) binding site in cultured mammary tissue.	the mouse mammary tumor virus (mmtv) initiates infection when the envelope protein, gp52, binds to a cell surface protein and triggers internalization. the hormonal regulation of this protein was studied both in vitro and in vivo. in mammary gland explant culture, levels of the mmtv binding protein were maintained only when prolactin was present in the medium. the further addition of progesterone induced levels to 164% of controls, while estradiol was without effect. these results were reproduce ...	1991	1665450
research priorities for diarrhoeal disease vaccines: memorandum from a who meeting.	diarrhoeas caused by rotaviruses, shigella, vibrio cholerae, and enterotoxigenic escherichia coli (etec) represent a major health burden in developing countries, and have stimulated much effort towards vaccine development in order to protect against these four disease agents. this memorandum describes the state of the art and points the way to future research and test trials in this area.	1991	1664785
are bunina bodies of endoplasmic reticulum origin? an ultrastructural study of subthalamic eosinophilic inclusions in a case of atypical motor neuron disease.	we carried out an electron microscopic study of eosinophilic intracytoplasmic inclusions in the subthalamic neurons in a case of atypical motor neuron disease. these inclusions were identical in light microscopic morphology and staining characteristics to bunina bodies. ultrastructurally, most of the intracytoplasmic inclusions observed were divisible into two different types (i and ii). type i inclusions had features essentially identical to those of bunina bodies demonstrated previously in the ...	2006	1664638
structure and function of inositol trisphosphate receptors.	inositol 1,4,5-trisphosphate (ins(1,4,5)p3) is a soluble intracellular messenger formed rapidly after activation of a variety of cell-surface receptors that stimulate phosphoinositidase c activity. the initial response to ins(1,4,5)p3 is a rapid ca2+ efflux from nonmitochondrial intracellular stores which are probably specialized subcompartments of the endoplasmic reticulum, although their exact identities remain unknown. this initial response is followed by more complex ca2+ signals: regenerati ...	1991	1663251
purification and properties of dna gyrase from vibrio cholerae.	the dna gyrase was purified from vibrio cholerae strain 569b. it appeared to be composed of two subunits of mr 120,000 and 97,000 and had a great deal of similarity to the escherichia coli gyrase. unlike the e. coli enzyme, however, it could neither relax supercoiled dna nor induce a cleavage of double-stranded dna, under experimental conditions in which e. coli gyrase could do so.	1991	1662488
[antibiotic sensitivity of el tor vibrio cholerae after exposure to various pesticides and mineral fertilizers].	the strains of el tor vibrio cholerae were exposed to different concentrations of pesticides (fazolone, treflane, prometrine, magnesium chlorate, omait and gardon) and mineral fertilizers (superphosphate, ammophos and carbamide) for 2 to 135 days. the subcultures of various ages were tested for their sensitivity to 16 antibiotics. the whole of 229 cultures were tested. there was a general tendency to lowering of the el tor vibrio sensitivity to the majority of the antibiotics tested. the vibrio ...	1991	1661574
chemical structure of the 2-keto-3-deoxyoctonate (kdo) region of the lipopolysaccharide isolated from o1 vibrio cholerae nih 4ir (ogawa).	the chemical structure of the 2-keto-3-deoxyoctonate (kdo) region of the lipopolysaccharide (lps) isolated from o1 v. cholerae nih 41r (ogawa) was elucidated by dephosphorylation, periodate oxidation and methylation analysis. methylation analysis of kdo in the dephosphorylated lps revealed the presence of 5-o-acetyl-1,2,4,6,7,8-hexa-o-methyl-3-deoxy-octitol and 2-keto-3-deoxy-heptulosonic acid was detected in the methanolysate of the periodate-oxidized and dephosphorylated lps. these results ind ...	1991	1661365
gene transfer in enteric bacteria through the formation of r-prime plasmids by an rp4: :mini-mu element.	gene transfer in seven pathogenic enteric bacteria was studied using an rp4: :mini-mu element, the plasmid pulb113. from the e. coli k-12 host strain the plasmid could be efficiently transferred to these enteric bacteria, but its transfer back to e. coli k-12 was not as efficient, being detected only in shigella dysenteriae 1, s. flexneri and the 'smooth' variant of s. sonnei. in these three species, transposition of chromosomal fragments into the plasmid to produce r-prime plasmid was also dete ...	1991	1661362
non-0:1 vibrio cholerae bacteremia: a case report and literature review.	we reported a case of non-0:1 group vibrio cholerae septicemia with myelodysplatic syndrome in taiwan. we also reviewed the other 22 reported cases of non-0:1 vibrion cholerae septicemia found in the literature regarding its pathogenesis and treatment. the case mortality rate of these 23 cases was 47.8%. most of them had immunocompromised diseases, particularly liver cirrhosis and hematologic malignancy. therefore, the most important factor is the host defense. the cholera-like enterotoxin and e ...	1991	1657343
[use of transposons for studying the genetic organization of vibrio cholerae non 01].	the nonagglutinating vibrions having tn-elements inserted into the chromosome were obtained as a result of conjugal transfer of vector plasmids carrying the different transposons (tn9, tn10, tn601, tn5-mob) and of the consequent isolation of plasmid-free clones of vibrio cholerae non oi. identification of auxotrophic mutations induced by the transposons inserted into the bacterial genome made possible the construction of the primary chromosomal map of vibrio cholerae non oi. the efficient donor ...	1991	1654521
regulation of leukotriene b4 generation from human polymorphonuclear granulocytes after stimulation with formyl-methionyl-leucyl phenylalanine: effects of pertussis and cholera toxins.	the effects of holotoxins and toxin subunits from bordetella pertussis and vibrio cholerae strains on intact and digitonin-permeabilized human polymorphonuclear neutrophils were studied. our data clearly demonstrate that formyl-methionyl-leucyl-phenylalanine (fmlp)-induced generation of chemotactic active leukotriene b4 was inhibited by both holotoxins as well as by their isolated enzymatic a protomers. in contrast, the respective binding components (b oligomers) did not affect leukotriene forma ...	1991	1652558
analysis of two genes encoding heat-labile toxins and located on a single ent plasmid from escherichia coli.	a clinical isolate of escherichia coli harbored two copies of the heat-labile toxin (lt)-encoding gene (elt) on a 157-kb plasmid. the arrangement of the gene copies is different from the cholera toxin-encoding gene duplication described for some strains of vibrio cholerae. the nucleotide sequences of the elt alleles are not identical (differing by 2 bp) and the duplicated region flanking the alleles extends 314 bp on one side and 1122 bp on the other side of each copy. different partial copies o ...	1991	1652542
interaction of ganglioside gm1 with the b subunit of cholera toxin modulates growth and differentiation of neuroblastoma n18 cells.	the present study uses the b subunit of cholera toxin, a protein that binds specifically to ganglioside gm1, to examine the role of endogenous gm1 in the process of growth and differentiation of mouse neuroblastoma n18 cells. binding of the b subunit to neuroblastoma n18 cells inhibited dna synthesis with concomitant induction of differentiation. the b subunit induced pronounced morphological changes: an increase in neurite outgrowth with branched neurites and spinelike processes. the distinct m ...	1991	1651376
cyclic amp- and inositol phosphate-independent inhibition of ca2+ influx by cholera toxin in cd3-stimulated jurkat t cells. a study with a cholera toxin-resistant cell variant and the ca2+ endoplasmic reticulum-atpase inhibitor thapsigargin.	in this report, we describe a jurkat cell variant, termed jct8, the selection of which is based upon its resistance to cell-growth inhibition mediated by the holotoxin of vibrio cholerae, cholera toxin (ct). jct8 cells exhibit normal camp production in response to various camp inducers, including ct, together with conserved adp ribosylation in vitro of g-protein gs alpha by the a subunit of the toxin. however, after a 4-h pretreatment with ct, jct8 cells have a conserved expression of cell-surfa ...	1991	1650386
evidence for insertion sequence-mediated spread of the thermostable direct hemolysin gene among vibrio species.	the tdh gene of vibrio parahaemolyticus which encodes the thermostable direct hemolysin has been found in some strains of other vibrio species. analysis of seven tdh genes cloned from v. parahaemolyticus, vibrio mimicus, and non-o1 vibrio cholerae revealed that all tdh genes were flanked by insertion sequence-like elements (collectively named isvs) or related sequences derived from genetic rearrangement of isvs. the isvs possessed 18-bp terminal inverted repeats highly homologous to those of is9 ...	1991	1650342
structural analysis of the acfa and acfd genes of vibrio cholerae: effects of dna topology and transcriptional activators on expression.	the vibrio cholerae acfa, b, c, and d genes are involved in the synthesis of a colonization factor; their expression is under the control of toxr, the cholera toxin transcriptional activator. by a combination of southern blot analysis, cloning, and nucleotide sequence analysis, we determined that the acf genes are clustered on a 5-kb region, the acfa and acfd genes are transcribed divergently, and the translation start sites of the two genes are separated by only 173 bp. expression from the acfa ...	1992	1644747
a one-minute oxidase test to detect vibrio strains isolated from cultures on thiosulphate-citrate-bile salts-sucrose (tcbs) medium.	vibrio cholerae is oxidase positive, a primary characteristic used to differentiate it from enterobacteriaceae. but false negative oxidase test results have been obtained with colonies from thiosulphate-citrate-bile salts-sucrose (tcbs) agar medium. a rapid oxidase test procedure is described here. this takes 1 min, avoids false negative results and the necessity to grow the bacteria in a general-purpose medium. the bacteria may be recovered after the test and used for further investigations.	1992	1644705
antibiotic susceptibilities of the vibrionaceae to meropenem and other antimicrobial agents.	the in vitro activity of meropenem was compared with imipenem and other selected antimicrobial agents against 115 isolates from the family vibrionaceae. no resistance was observed with meropenem or imipenem against these isolates. however, meropenem was generally four- to 16-fold more active than imipenem against the aeromonads and vibrio cholerae. meropenem showed excellent in vitro activity against the vibrionaceae and may be useful for eradicating infections produced by these organisms.	1992	1643823
identification of a lacz gene in vibrio cholerae.	plasmids that express an enzymatically active beta-galactosidase in an escherichia coli delta lac strain were isolated from libraries of recombinant plasmids containing vibrio cholerae chromosomal dna. deletion analysis localized the gene responsible for beta-galactosidase activity on a 3.1-kb dna fragment, and the gene product was identified as a protein of approximately 110 kda. primary sequence comparisons indicated that this v. cholerae gene is homologous to the e. coli lacz gene. in contras ...	1992	1641510
synthesis, characterization, and some immunological properties of conjugates composed of the detoxified lipopolysaccharide of vibrio cholerae o1 serotype inaba bound to cholera toxin.	protection against cholera has been correlated with the level of serum vibriocidal antibodies. the specificity of these vibriocidal antibodies was mostly to the lipopolysaccharide (lps). we synthesized conjugates of detoxified lps with cholera toxin (ct) and other proteins in order to elicit serum lps antibodies with vibriocidal activity. treatment with hydrazine (deacylated lps) reduced the endotoxic properties of the lps to clinically acceptable levels and resulted in a molecule larger and mor ...	1992	1639490
adaptive response of vibrio cholerae and escherichia coli to nitrofurantoin.	pretreatment with sublethal doses of nitrofurantoin induced adaptive response in both vibrio cholerae and escherichia coli cells as indicated by their greater resistance to the subsequent challenging doses of the same drug. adaptive response was maximum corresponding to pretreatment drug concentrations of 0.40 microgram/ml and 0.015 microgram/ml respectively for v. cholerae ogawa 154 (wild type) and e. coli k-12 ab 2463 (reca-) cells. adaptive response was inhibited by chloramphenicol (100 micro ...	1992	1639084
epidemiological survey of an outbreak of gastroenteritis in a rural community in oyo state.	this report documents the investigation and control of an outbreak of gastro-enteritis in lagun, oyo state, nigeria. prior to the outbreak which occurred in 1981, there had been epidemics of gastro-enteritis, suspected to be cholera, in various parts of nigeria. the investigations carried out included personal interviews of patients and their contacts, laboratory investigations of faecal specimens and analysis of water samples obtained from the village wells and stream. available evidence indica ...	1992	1637741
prospective, controlled, double-blind study of i.v. tenoxicam for analgesia after thoracotomy.	we have shown that a single i.v. dose of tenoxicam 20 mg, after thoracotomy, when compared with placebo in 20 patients (with one exclusion), was associated with a reduction in consumption of papaveretum, assessed by patient-controlled analgesia, of 2.2 mg h-1 (22%) to 4 h and 1.4 mg h-1 (23%) to 12 h after operation (repeated measures analysis of variance: p less than 0.01). there was no reduction from 12 to 24 h. there was no significant difference between groups in pain scores or in side effec ...	2006	1637611
presumed exudative retinal detachment after cryotherapy in retinopathy of prematurity.	cryotherapy of the peripheral avascular retina in selected cases of retinopathy of prematurity has been shown to reduce the incidence of posterior retinal detachment, retinal fold involving the macula, and retrolental tissue. although exudative retinal detachments have been described after cryotherapy during scleral buckling procedures, to our knowledge, this observation has not been reported after cryotherapy in retinopathy of prematurity. we describe such a case.	2006	1637126
[eye infections--a red eye can indicate infection].		1992	1636299
[eye foreign bodies. patients should be sent to the eye clinic for treatment].		2006	1636298
[shstf acts against racism and hostility toward strangers].		2006	1636291
[treatment procedure in humeral shaft fractures with primary or secondary radial nerve damage].	we give an account of 28 patients with fractured humerus diaphysis, suffering from primary and seven patients suffering from secondary lesion of the radialis nerve, all treated by operation. in each case the radialis nerve was exposed and the fracture was treated by fixation with compression plates. in case of primary lesions of the radialis nerve, we found intraoperative damages by traction (six times), contusions (seven times), intraneural hämatomas (six times) and for one time perforation of ...	2006	1636222
atp-sensitive k+ channels mediate an ipsp in dorsal horn neurones elicited by sensory stimulation.	nociceptive dorsal horn neurones, which are involved in the processing of pain-related information, are inhibited by input from vibration-sensitive, large diameter primary sensory fibres (wall and cronly-dillon, 1960; salter and henry, 1990a,b). we have reported previously that the inhibition of spinal nociceptive neurones by vibration is mediated by adenosine acting through p1-purinergic receptors (salter and henry, 1987). in a number of different types of cell, adenosine is known to activate k ...	1992	1636151
total hepatic mesh wrap for hemostasis.	to improve hemostasis and avoid the complications of perihepatic packing, a technique of hepatic tamponade using total mesh wrap is reported. the method is geometrically, technically and mechanically feasible and appears to be effective in controlling severe parenchymatous bleeding.	1992	1636144
treatment of calculi of the common bile duct.	endoscopic sphincterotomy (est) is regarded as an alternative therapeutic approach to the surgical treatment of choledocholithiasis. to clarify the indication for each of these two methods, a prospective study has been performed comparing patients with calculi of the common bile duct who had undergone endoscopic or surgical treatment. mortality, morbidity and stone clearance were used as criteria for the evaluation of the dependence of age on results achieved by the two modalities. of 306 patien ...	2006	1636134
[pharmacology and action mechanism of endothelium-derived relaxing factor].		2006	1635181
[differentiation therapy of acute promyelocytic leukemia: two successful cases of remission induction by all-trans retinoic acid].	we described two pediatric patients with acute promyelocytic leukemia (apl) who were successfully induced into complete remission with all-trans retinoic acid (atra, 45 mg/m2 per day) after failing on conventional chemotherapy. initial response was observed as correction of dic within a week of treatment. hematologically, initial increase of maturing leukocytes reached a peak peripheral wbc count on the 16th and 20th day, respectively. however, these seemingly differentiated leukocytes retained ...	2006	1635178
[hairy cell leukemia successfully treated with 2'-deoxycoformycin following refractoriness to splenectomy and alpha-interferon therapy].	a 34-year-old man was admitted in may, 1989 because of severe left upper abdominal pain, which was caused by advanced splenomegaly. on initial examinations, peripheral blood showed leukocytosis (13,400/ml) including 60% hairy cell which also infiltrated in bone marrow (64%). the patient was diagnosed as having hairy cell leukemia (japanese type) of b-cell lineage. splenectomy was performed as an initial treatment. the effect of splenectomy was only palliative and transient leukocytosis progresse ...	1992	1635172
ellis-van creveld syndrome.		2006	1634791
[the cholera epidemic in latin america].	an outbreak of cholera started in peru in january 1991 and spread through most latin american countries within a year. this was the first known epidemic of cholera in america for more than a century. in 1991, 321,334 persons were reported to have cholera in peru, 119,063 were hospitalized, and 2,906 died. other countries like ecuador, colombia, guatemala, brazil, mexico, bolivia, chile, el salvador, venezuela and honduras were also affected, but these countries combined accounted for only 20% of ...	1992	1631846
characterization of a periplasmic thiol:disulfide interchange protein required for the functional maturation of secreted virulence factors of vibrio cholerae.	a number of toxr-regulated genes that encode products required for the biogenesis or function of the toxin-coregulated colonization pilus (tcp) of vibrio cholerae have been identified previously by tnphoa fusions. in this study we have examined the role of the product of one of these genes, tcpg, to which a fusion results in a piliated cell lacking all of the in vivo and in vitro functions associated with tcp. our results show that tcpg is not an ancillary pilus adhesin component as suggested by ...	1992	1631111
evaluation of the bead enzyme-linked immunosorbent assay for detection of cholera toxin directly from stool specimens.	a highly sensitive bead enzyme-linked immunosorbent assay (bead elisa) for detection of cholera toxin (ct) was evaluated for direct detection of ct from stool specimens of patients with acute secretory diarrhea. of the 75 stool samples examined, 59 yielded biochemically, and serologically confirmed strains of vibrio cholerae o1. the bead elisa was positive for ct in stool supernatants in 50 (84.7%) of the 59 samples from which v. cholerae o1 was isolated. in addition, the bead elisa was positive ...	1992	1629335
[photodynamically induced vitreous liquefaction in vivo].	photodynamically induced vitreous liquefaction in rabbit eye was investigated. photosensitizer, riboflavine phosphate, was injected into the vitreous cavity of the rabbit before white-light irradiation. after the irradiation (0, 1, 3, 6 hr) the rabbit vitreous body was separated into gel and liquid portions. the liquid vitreous body was weighed, and the vitreous liquefaction percentage was calculated. one hour irradiation caused 38% of liquefaction of the eye; 3 hr, 50% liquefaction; 6 hr, 59% l ...	1992	1626474
[current insights on the pathogenesis of chronic inflammatory bowel diseases].	the pathogenesis and etiology of inflammatory bowel disease (ibd) is poorly understood. as a matter of fact, it is not even certain whether either one is a single entity with different forms of clinical manifestations, or whether each one represents a single clearly separable entity. common features of both diseases are chronic persistence, recurrent exacerbation and remission, the production of autoantibodies, as well as the expression of aberrant hla-class ii molecules on the surface of epithe ...	1992	1626162
[cryptococcosis in aids patients: a study of 19 cases].	we discuss 19 cases of infection due to cryptococcus neoformans diagnosed in 438 aids patients admitted to our center (4%). fourteen of them showed meningitis confirmed by culture of c. neoformans in csf. clinical features were rather unspecific and disorders in csf parameters were non striking. the diagnostic techniques performed with best results were culture of c. neoformans and antigen determination, especially in serum. survival probability at one year was 75%. treatment response was good. ...	1992	1626090
review of behavior on a disk from cms academic software: instructional programs for teaching teachers.	the programs on boad represent a set of useful simulations and demonstrations of learning phenomena that successfully convey important practical and theoretical information to students. the most successful modules deal with shaping and the selective effect of positive reinforcement on behavior. the range of examples is sufficiently broad to convey the generality of these learning phenomena, but the graphics and particular examples are better suited to the college classroom (for which they were d ...	1992	1626085
acquisition, generalization, and spontaneous use of color adjectives: a comparison of incidental teaching and traditional discrete-trial procedures for children with autism.	incidental teaching and traditional discrete-trial procedures were used to teach two children with autism the expressive use of two color adjectives to describe preferred toys and food items. the two teaching procedures were performed in a classroom setting, and generalization and spontaneous usage were assessed at home with parents. the results demonstrated that traditional discrete-trial teaching was more efficient and produced faster acquisition and, initially, greater generalization. however ...	2006	1626079
isolation of vibriostatic agent o/129-resistant vibrio cholerae non-o1 from a patient with gastroenteritis.	a strain of vibrio cholerae non-o1 was isolated from a 48-year-old male in california who presented with a chief complaint of watery diarrhea. laboratory investigations of this strain revealed the bacterium to be resistant to trimethoprim-sulfamethoxazole and to the vibriostatic agent o/129 (2,4-diamino-6,7-diisopropylpteridine).	1992	1624583
control of the transmission of vibrio cholerae and other enteropathogens by foods originating from endemic areas in south america and elsewhere as a model situation.	the cholera-pandemic raging in south and middle america and endemic cholera in other countries call for measures of health protection of the local population, but particularly with respect to the young, old, pregnant and immunocompromised citizens of countries importing food from the areas where the disease has struck. instead of harshly barring importation, a more humanitarian policy is recommended, relying on assistance of areas presenting risks, with the introduction of and adherence to rigor ...	1992	1622746
clinical and bacteriological studies of el tor cholera after ingestion of known inocula in thai volunteers.	twenty-six healthy adult thai volunteers were recruited for clinical and bacteriological studies of cholera induced by oral inoculation with vibrio cholerae el tor inaba strain n16961. vibrio dosages of 0.3 x 10(4), 1.6 x 10(5) and 1.9 x 10(6) c.f.u. were given to three groups of five volunteers, and 2.0 x 10(7) c.f.u. to 11 volunteers. diarrhoeal attack rates correlated positively with the size of the inocula (p less than 0.01). it was estimated that a diarrhoeal attack rate of 90% (ed90) would ...	1992	1621412
[fundus plication with or without proximal selective vagotomy?].	the aim of this study was to determine the influence of proximal gastric vagotomy on the outcome after fundoplication. of 141 patients operated on for reflux disease between 1972 and 1988, 53 had fundoplication alone (group a) and 88 fundoplication combined with proximal gastric vagotomy (group b). the two groups were similar with regard to the severity of the reflux disease (esophagitis: a 69%, b 73%) but had a different incidence of concomitant ulcer disease (a 2%, b 55%). after a mean follow- ...	2006	1621082
[the cholera epidemic in panama 1991].	the authors studied the epidemiology of the outbreak of cholera (due to vibrio cholerae 01, inabe serotype) which occurred in panama in 1991, during which 1179 probable cases were reported, with 292 hospitalizations, 29 deaths and 51 confirmed cases. there was an average of 24.7% hospitalizations and a 2.5% mortality. the epidemic involved 21 of 24 "corregimientos' in darién, 2 of 4 in san blas, 5 of 15 in panama east, 3 of 40 in colón and one of 19 in the metropolitan area of panama city. the i ...	1992	1620896
high-performance liquid chromatographic method to resolve and determine lipopolysaccharide sub-groups of escherichia coli endotoxin in isolated perfused rat liver perfusate.	a high-performance liquid chromatographic method was developed for resolving heterogeneous preparations of fluorescently labelled endotoxin derived from escherichia coli (serotype 0111:b4) into separate lipopolysaccharide sub-groups. the endotoxin was chromatographed on an analytical gel permeation column using a mobile phase of acetonitrile (20%, v/v) and 100 mm phosphate buffer (ph 7.75). four fluorescent peaks were resolved, representing sub-groups of markedly different molecular sizes. three ...	1992	1618951
the segregation of a translocation t(1;4) in two male carriers heterozygous for the translocation.	we examined the meiotic segregation pattern of a t(1;4)(p36.2;q31.3) reciprocal translocation in two male cousins heterozygous for the translocation. the wife of subject 1 had four recognized spontaneous abortions and two carrier daughters, and the wife of subject 2 had three recognized spontaneous abortions and no live-born children. the results showed that subject 1 had an imbalance rate of 54% and subject 2 had an imbalance rate of 61% with respect to the translocation. this was not statistic ...	1992	1618491
the segregation of a translocation t(1;4) in two male carriers heterozygous for the translocation.	we examined the meiotic segregation pattern of a t(1;4)(p36.2;q31.3) reciprocal translocation in two male cousins heterozygous for the translocation. the wife of subject 1 had four recognized spontaneous abortions and two carrier daughters, and the wife of subject 2 had three recognized spontaneous abortions and no live-born children. the results showed that subject 1 had an imbalance rate of 54% and subject 2 had an imbalance rate of 61% with respect to the translocation. this was not statistic ...	1992	1618491
neonatal exposure to protoporphyrin-activating lighting as a contributing cause of childhood acute lymphocytic leukemia.	while being a relatively rare disease, acute lymphocytic leukemia (all) is the leading form of cancer in children in the developed world today. all sharply peaks in incidence at ages three to four years. in the united states there have been persistent, unexplained increases in incidence of all in the past two decades. we hypothesize that exposure to photosensitizing lighting immediately after birth may be a contributing cause of all. fluorescent lamps and other light sources with strong illumina ...	2006	1617127
[morbidity caused by industrial accidents in a health zone].	the aim of the present study is to discover the morbidity produced by workplace accidents among the earning population of molina de segura health area.	1992	1617092
prostatic hemangiosarcoma in a dog: clinical and pathologic findings.		1992	1616992
posterior inferior cerebellar artery (pica) aneurysm presenting with sah and contralateral crural monoparesis: a case report.	a patient with contralateral monoparesis of the leg due to subarachnoid hemorrhage (sah) from an aneurysm of the first posterior inferior cerebellar artery (pica) segment is reported. the monoparesis may well be associated with the close anatomical relationships between the site of the aneurysm and the pica blood supply of the corticospinal fibers to the contralateral leg.	1994	1615373
accumulation of copper, lead, manganese and iron by field populations of hydrodictyon reticulatum (linn.) lagerheim.	the potential of 'water-net' hydrodictyon reticulatum to accumulate copper, lead, manganese and iron was determined in seven polluted water bodies having different physicochemical characteristics. the lead accumulation was linearly related with ambient concentration whereas in case of copper, manganese and iron it was maximum at lowest ambient level of metals. investigations on response of alga to various concentrations of test metals under single metal treatments revealed that the algal cells a ...	1995	1615306
characterization of borrelia burgdorferi glycoconjugates and surface carbohydrates.	borrelia burgdorferi glycoconjugates with different oligosaccharide structures were characterized by a blotting technique with peroxidase-labelled lectins. the localization of surface carbohydrates was studied using electron microscopy with lectin-gold complexes. a high-mannose glycan structure was detected in 83 kda glycoprotein (major extracellular protein); at least four carbohydrates (glucose or mannose, galactose, n-acetylgalactosamine and n-acetylglucosamine) were present in other borrelia ...	1992	1611204
how accurate is a computerized surgical audit when resident medical staff collect the data?	since july 1988 all eight general surgeons at fremantle hospital have used a computer-based surgical audit and discharge system. at the time of writing (september 1991) 10,919 computer-generated discharge letters have been produced by the system. this paper describes the system and reports a series of quality control assessments carried out between 1 july 1988 and 30 june 1990 during which 30 pre-registration surgical residents completed 5,716 data collection forms. it was found that: (1) data c ...	2006	1610325
[ataxia telangiectasia].		2006	1610280
plastic ampoules.		2006	1609921
[diseases caused by non-o1 vibrio cholerae contaminated food].		1992	1606871
multiple myeloma--a curable disease?		2004	1602281
selective extracellular release of cholera toxin b subunit by escherichia coli: dissection of neisseria iga beta-mediated outer membrane transport.	the c-terminal domain (iga beta) of the neisseria iga protease precursor is involved in the transport of covalently attached proteins across the outer membrane of gram-negative bacteria. we investigated outer membrane transport in escherichia coli using fusion proteins consisting of an n-terminal signal sequence for inner membrane transport, the vibrio cholerae toxin b subunit (ctxb) as a passenger and iga beta. the process probably involves two distinct steps: (i) integration of iga beta into t ...	1992	1600950
cholinergic activity and constraints on information processing.	in humans, close relationships are found between cholinergic activity and constraints placed on information processing operations. this is true for all operations where the effects of cholinergic activity have been studied. studies of vigilance, memory, problem solving, stimulus processing and response processing are cited as illustrations. these studies suggest the hypothesis that cholinergic activity controls constraints in all information processing operations. alternative hypotheses are prop ...	2006	1599996
radiological application of three-dimensional imaging systems.	three-dimensional displays are becoming more frequent in medical imaging. as pacs and imaging workstations as nodes on networks provide greater ease and availability of three-dimensional reconstructions of digital data applications are increasing. applications at this time are limited to high-contrast structures. in ct these are complex fractures or deformities. three-dimensional display clarifies the anatomy and facilitates operative planning. in mri, mr angiography is the principal application ...	2006	1599735
peroxidase-amplified assay of sialidase activity toward gangliosides.	sialidase assays were carried out with the substrate, ganglioside gd1a, coated onto enzyme immunoassay plate wells. following the incubation of gd1a with sialidase from v. cholerae, the amount of ganglioside gm1 produced was measured as follows: cholera toxin b subunit conjugated to horseradish peroxidase was added to specifically bind to gm1, and then the amount of bound peroxidase was determined in a colorimetric enzymatic assay. in the absence of detergent, linearity for the detection of gm1 ...	1992	1595901
the relationship of anticoagulation level and complications after successful percutaneous transluminal coronary angioplasty.	the degree of anticoagulation and its effect on the frequency of abrupt coronary artery closure, coronary ischemia, bleeding complications requiring transfusion, and death were examined in 336 patients after elective percutaneous transluminal coronary angioplasty (ptca). all patients received a bolus of 10,000 u of heparin at the beginning of the procedure followed by a continuous infusion of 2000 u/hr. at the conclusion of the procedure the infusion was reduced to 1000 u/hr and continued for 18 ...	2006	1595522
common infections in the elderly.	symptoms of infection in the elderly may be absent, vague or atypical. infection should be suspected when an elderly patient presents with a decline in well-being or with non-specific symptoms such as falls, dizziness, confusion, anorexia or weakness. common infections include bacterial pneumonia, urinary tract infection, intra-abdominal infections, gram-negative bacteremia and infection of decubitus ulcers. antibiotic therapy is not recommended for asymptomatic bacteriuria or locally infected d ...	2006	1595517
diary from a week in practice.		2006	1595508
the ventriculofugal arteries.		2006	1595488
partial albinism with immunodeficiency: a rare syndrome with prominent posterior fossa white matter changes.	to describe our experience in infants with partial albinism and immunodeficiency (paid), a rare, recently recognized, probably autosomal recessive disorder.	2006	1595481
sequential cranial mr findings of asymptomatic and neurologically symptomatic hiv+ subjects.	to compare results of a prospective mr and clinical reevaluation of hiv+ asymptomatic and neurologically symptomatic subjects who had had initially abnormal cranial studies to determine what cranial mr changes occur and how these changes correlate with serial neurologic and neuropsychologic findings.	1992	1595476
pontine glioma extending to the ipsilateral cavernous sinus and meckel's cave: mr appearance.	the authors describe an exophytic glioma of the pons that grew into the meckel's cave and cavernous sinus in a 75-year-old man. pontine gliomas should be included in the differential diagnosis of a hyperintense, complex cystic mass seen along the distribution of cranial nerve v.	1992	1595473
experience with high-dose gadolinium mr imaging in the evaluation of brain metastases.	to assess the effectiveness and safety of higher doses of gadoteridol in the mr evaluation of patients with brain metastases.	2006	1595472
ossification of the posterior cervical longitudinal ligament.	forty patients with cervical myelopathy due to opll (ossification of the posterior longitudinal ligament) of the cervical spine were studied. according to abe's or yamamoto's classification, 12 of them had a 50% decrease in the cross-sectional area of the spinal canal. subtotal vertebrectomy was carried out in 8 of these patients and the remaining 4 patients received posterior decompression. we concluded that anterior decompression, if possible, is the treatment of choice and posterior decompres ...	2006	1595391
[poisoning at a polyvalent intensive care unit].	a retrospective analysis was made concerning 1340 admissions to a multidisciplinary intensive care unit over four years (1986-1989), because of acute poisoning. in 666 patients occurred 735 drug overdoses and in 674 patients, 691 non-drug intoxications. the drugs with main action in central nervous system were the most frequent in drug overdoses and the organophosphates insecticides in the non-drug intoxications. during the considered period there was an absolute and relative fall in the number ...	2006	1595377
occupational exposure to carbon monoxide during charcoal meat grilling.	charcoal meat grill workers are one of the many occupational groups that are subject to carbon monoxide exposure. this group have often been overlooked and not investigated. carboxyhemoglobin (%cohb) levels in 100 male workers were assessed before work and after work the same day. carboxyhemoglobin levels increased significantly after work in both smoking and nonsmoking workers. the mean cohb levels for smoking workers before work was 3.8% and for nonsmokers was 2.4%, whereas after work, the mea ...	2006	1594919
[the importance of applied ethology for the execution of animal welfare legislation].	applied ethology in general and farm animal ethology in particular have a great importance in connection with animal welfare regulations on a national and international level. they have through the legislation on animal welfare brought about important repercussions on housing of farm animals, wild animals and experimental animals in switzerland. the animal welfare legislation has been a considerable boost to scientific research. the application of ethological knowledge in the legislation and in ...	2006	1594916
[obesity. various aspects in daily practice].	overweight and obesity are the most often encountered disease conditions in general practice. recent evidence of the importance of fat distribution is discussed. the basic therapeutic approach for the most effective therapy of obesity is probably the combination of a dietary (hypocaloric), a behavioral and a physical activity approach. in this general overview the physiologic background of abdominal obesity and some selected therapeutic implications are discussed.	2006	1594849
[headache, fever and behavior problems. lymphoma].		2006	1594826
[fetomaternal alloimmunization: role of cordocentesis].	twenty one pregnancies complicated by alloimmunization were managed by the use of intravascular method on an outpatient basis. one group was made of 9 women having had at least one pregnancy with a severely affected fetus. the other group was composed of 12 women without a previously affected infant; in 5 cases a situation at risk, either a transfusion (4 cases) or a severe obstetrical hemorrhage (1 case), was evidenced. knowledge of fetal blood type (2 cases) and hematocrit determination obtain ...	2006	1594825
applying total quality management concepts to public health organizations.	total quality management (tqm) is a participative, systematic approach to planning and implementing a continuous organizational improvement process. its approach is focused on satisfying customers' expectations, identifying problems, building commitment, and promoting open decision-making among workers. tqm applies analytical tools, such as flow and statistical charts and check sheets, to gather data about activities within an organization. tqm uses process techniques, such as nominal groups, br ...	2006	1594734
supporting health care workers in the treatment of hiv-infected patients.	the care of hiv-infected patients is demanding, raising concerns among health care workers regarding safety, competence, and emotional endurance. many health care workers are reluctant to undertake this challenging work. for many professions within health care, for example nursing, there is a clearly articulated responsibility to treat all ill individuals. less unanimity on this point continues among physicians. clearly, a first issue is to continue the discussion of the nature of physicians' pr ...	1992	1594701
analysis of the relationships between self-stimulation sniffing and brain-stimulation sniffing.	determination of current thresholds for self-stimulation and electrically elicited sniffing from electrodes placed into lateral hypothalamic and ventral tegmental areas of rats revealed a tight correlative relationship between the two phenomena (r values of approx 0.9 at both sites). thresholds for sniffing were never higher than those for self-stimulation, while approximately half the animals had higher self-stimulation than sniffing thresholds, suggesting that electrically elicited sniffing ma ...	1992	1594679
subtotal lesions of the amygdala: the rostral central nucleus in passive avoidance and ulceration.	rats received small bilateral electrolytic or ibotenate lesions of the rostral part of the amygdaloid central (race) or lateral (ral) nuclei, or caudal part of the basolateral nuclei (cbl), or electrolytic lesions of the dorsal hippocampus (hipp). all groups were tested in a drinking passive avoidance (pa) task that appears less sensitive to deficits in acquisition/retention or activity/spatial perception than are many other pa tasks, and more specifically sensitive to deficits in generation of ...	1992	1594678
optimal protein-folding codes from spin-glass theory.	protein-folding codes embodied in sequence-dependent energy functions can be optimized using spin-glass theory. optimal folding codes for associative-memory hamiltonians based on aligned sequences are deduced. a screening method based on these codes correctly recognizes protein structures in the "twilight zone" of sequence identity in the overwhelming majority of cases. simulated annealing for the optimally encoded hamiltonian generally leads to qualitatively correct structures.	2006	1594594
the highest gene concentrations in the human genome are in telomeric bands of metaphase chromosomes.	chromosome in situ suppression hybridization has been carried out on human metaphase chromosomes to localize the g+c-richest human dna fraction (which only represents 3.5% of the genome), as isolated by preparative equilibrium centrifugation in cs2so4/3,6-bis(acetatomercurimethyl)-1,4-dioxane density gradient. this fraction essentially corresponds to isochore family h3. the rationale for carrying out this experiment is that this isochore family has, by far, the highest gene concentration, the hi ...	1992	1594593
determination by systematic deletion of the amino acids essential for catalysis by ricin a chain.	the a chain of ricin is a rna n-glycosidase that inactivates ribosomes by depurination of a single adenosine in 28s rrna. of the 267 amino acids in the protein, 222 (83%) could be deleted from one or another of 74 mutants without loss of the capacity of the protein to recognize a single nucleotide from among the 7000 in ribosomes or to catalyze hydrolysis.	2006	1594586
aetiological studies on hospital inpatients with secretory diarrhoea in calcutta.	twenty-five cases of all age groups with secretory diarrhoea admitted to the infectious diseases hospital, calcutta were studied. bacterial enteropathogens were detected by bacteriological examination of the faeces, in each and every case. the main pathogens detected were escherichia coli and vibrio cholerae. v cholerae was more commonly associated with severe illness except in the very young, while esch coli was equally associated with moderate and severe illness. multiple isolates were more co ...	1992	1593139