Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| influence of protein conformation on disulfide bond formation in the oxidative folding of ribonuclease t1. | in oxidative protein folding the interdependence between the acquisition of an ordered native-like conformation and disulfide bond formation was investigated by using the c2s/c10n variant of ribonuclease t1 as a model. this protein of 104 residues has a single disulfide bond between cys6 and cys103. in the reduced form it is unfolded in the presence of urea, but native-like folded when > or = 1.5 m nacl is present. the influence of a folded conformation on the individual thiol/disulfide exchange ... | 1995 | 7643382 |
| isolation and characterization of the gene encoding the surface membrane 3'-nucleotidase/nuclease of leishmania donovani. | leishmania donovani and related trypanosomatid protozoa possess an externally oriented surface membrane enzyme capable of hydrolyzing both 3'-nucleotides and nucleic acids. by virtue of these activities, this 3'-nucleotidase/nuclease (3'-nt/nu), previously shown to be analogous to fungal and plant class-i single-strand-specific nucleases, is thought to play a critical role in the salvage of purines, essential for the survival of these organisms. the 43-kda 3'-nt/nu was purified from l. donovani ... | 1995 | 7630383 |
| effects of lactitol-oligosaccharides on the intestinal microflora in rats. | lactitol-oligosaccharide (lo) was prepared from lactitol by a transgalactosylation reaction catalyzed by aspergillus oryzae beta-galactosidase. the utilization of lo by human intestinal bacteria, the digestion of lo by rat jejunum mucosal homogenates and the effects of lo on the intestinal microflora in rats were compared with those of lactitol. 1) lo was utilized in vitro by bifidobacterium, but lactitol was not utilized. 2) neither lo nor lactitol were digested by rat jejunum mucosal homogenat ... | 1995 | 7616329 |
| skin-prick tests for hypersensitivity to alpha-amylase preparations. | twenty-five asthmatic subjects with suspected alpha-amylase hypersensitivity were studied by skin-prick tests, a capture elisa, immunoblotting and bronchial provocation tests. at the same time, different amylases were analysed by sds-page and immunoblotting using a polyclonal rabbit antiserum. eight patients showed a positive bronchial response to amylase. seven of them had positive skin-prick tests, with this method being the most sensitive approach for diagnosis. however, in four cases, skin t ... | 1995 | 7605978 |
| molecular cloning and characterization of a rhamnogalacturonan acetylesterase from aspergillus aculeatus. synergism between rhamnogalacturonan degrading enzymes. | a rhamnogalacturonan acetylesterase (rgae) was purified to homogeneity from the filamentous fungus aspergillus aculeatus, and the nh2-terminal amino acid sequence was determined. full-length cdnas encoding the enzyme were isolated from an a. aculeatus cdna library using a polymerase chain reaction-generated product as a probe. the 936-base pair rha1 cdna encodes a 250-residue precursor protein of 26,350 da, including a 17-amino acid signal peptide. the rha1 cdna was overexpressed in aspergillus ... | 1995 | 7592973 |
| induction and repression of alpha-amylase production in batch and continuous cultures of aspergillus oryzae. | the intra- and extracellular concentrations of alpha-amylase in aspergillus oryzae have been measured during batch culture of a wild-type strain and two recombinant strains. the mean intracellular level for the two recombinant strains was about four to five times the level of the wild-type strain. the recombinant strains also had a higher alpha-amylase productivity, whereas the residence time of the intracellular alpha-amylase pool was approximately the same for the three strains. at high glucos ... | 1995 | 7582005 |
| an acetylglucomannan esterase of aspergillus oryzae; purification, characterization and role in the hydrolysis of o-acetyl-galactoglucomannan. | an acetyl glucomannan esterase (agme) was purified to electrophoretic homogeneity from the culture supernatant of aspergillus oryzae. this new enzyme had a molecular mass of 36 kda and an isoelectric point of 4.6. it was most active in the ph range 5.0-5.5 and was stable for 24 h at 40 degrees c at ph 5.0-6.0. the purified esterase liberated acetic acid from o-acetyl-galactoglucomannan, o-acetyl-4-o- methylglucuronoxylan and alpha-naphtyl acetate. the specific activity was 10-times higher for ac ... | 1995 | 7576539 |
| isolation and characterization of polygalacturonase genes (peca and pecb) from aspergillus flavus. | two genes, peca and pecb, encoding endopolyglacturonases were cloned from a highly aggressive strain of aspergillus flavus. the peca gene consisted of 1,228 bp encoding a protein of 363 amino acids with a predicted molecular mass of 37.6 kda, interrupted by two introns of 58 and 81 bp in length. accumulation of peca mrna in both pectin- or glucose-grown mycelia in the highly aggressive strain matched the activity profile of a pectinase previously identified as p2c. transformants of a weakly aggr ... | 1995 | 7574642 |
| molecular cloning of the cdna and gene for an elastinolytic aspartic proteinase from aspergillus fumigatus and evidence of its secretion by the fungus during invasion of the host lung. | hydrolysis of structural proteins in the lung by extracellular proteinases secreted by aspergillus fumigatus is thought to play a significant role in invasive aspergillosis. this fungus was found previously to secrete an elastinolytic serine proteinase and a metalloproteinase. we report that a. fumigatus also secretes an aspartic proteinase (aspergillopepsin f) that can catalyze hydrolysis of the major structural proteins of basement membrane, elastin, collagen, and laminin. the ph optimum for t ... | 1995 | 7558282 |
| gene transfer by electroporation of filamentous fungi. | 1995 | 7550746 | |
| nucleotide sequence and expression of alpha-glucosidase-encoding gene (agda) from aspergillus oryzae. | we have isolated an alpha-glucosidase(agl)-encoding gene (agda) from aspergillus oryzae by heterologous hybridization using the corresponding aspergillus niger gene as a probe. southern hybridization analysis showed that the agda gene is on a 5.0-kb scai fragment and there is a single copy in the a. oryzae chromosome. comparison with the a. niger agda gene indicated that the agda gene contains three putative introns from 52 to 59 nucleotides long, and that it encodes 985 amino acid residues. the ... | 1995 | 7549103 |
| cloning and nucleotide sequence of the calmodulin-encoding gene (cmda) from aspergillus oryzae. | a cdna and genomic gene encoding calmodulin were isolated from aspergillus oryzae using a part of the calmodulin gene from a. nidulans as a hybridization probe. the gene was in a 3.4-kb sphi fragment and southern-blot analysis of genomic dna suggested the existence of a single copy of the calmodulin gene in a. oryzae. the nucleotide sequence analysis showed that the gene consists of five introns and six exons. although the nucleotide sequence homology with that of a. nidulans was not so high (68 ... | 1995 | 7549095 |
| serological responses induced in mice by immunogenic proteins and by protein respiratory allergens. | it is known that a variety of materials, including both low molecular weight chemicals and proteins, is able to induce occupational respiratory allergy. we have shown previously that exposure of mice to chemical respiratory sensitizers results in both a marked increase in the serum concentration of ige and the appearance of specific ige antibody. in the present study we have examined the characteristics of immune responses induced in mice following intraperitoneal exposure to 3 protein respirato ... | 1994 | 7518969 |
| effect of various commercially available enzymes in the liquid chromatographic determination with external standardization of thiamine and riboflavin in foods. | the efficacy of various commercially available enzymes in the determination of thiamine and riboflavin in foods was studied by liquid chromatography (lc) using external standards. different enzymes, as well as the same enzyme produced by different manufacturers, very strongly affected the determination of both vitamins. the recoveries for different foods ranged from 85 to over 100% for thiamine and from 80 to 100% for riboflavin. the present lc method was accurate and precise when tested on a fo ... | 1994 | 7516753 |
| homology between prokaryotic and eukaryotic ribonucleases. | there is homology between the amino acid sequences of the extracellular ribonucleases t1 and st, from the eukaryote aspergillus oryzae and the prokaryote streptomyces erythreus, respectively. together with other extracellular ribonucleases homologous to each, these enzymes make up a family of interest to evolutionary biology and useful in studies of protein structure and function. | 1980 | 7411658 |
| [influence of the sporulation on glucose-6-phosphate dehydrogenase activity from aspergillus oryzae (ahlburg) (author's transl)]. | both the specific and the total activity of glucose-6-phosphate dehydrogenase (e.c. 1.1.1.49) of mycelium of aspergillus oryzae decline progressively from day 6th of incubation, when the period of sporulation begins. total cellular protein decreases parallely. cicloheximide stops protein synthesis but not the inactivation of glucose-6-phosphate dehydrogenase. inhibitors of proteolytic enzymes do not prevent the loss of enzyme activity when added to the cultures. experiments show no inhibitors of ... | 1980 | 7403645 |
| [physicochemical and enzymatic properties of aspergillus oryzae aminopeptidase]. | the paper deals with properties of aspergillus oryzae (strain kc) purified aminopeptidase. the enzyme is homogeneous in electrophoresis in polyacrylamide gel and enzyme-electrophoresis with the synthetic substrate leucyl-beta-naphthylamide applied. the molecular mass is 60000-61000 daltons. the amino acidic composition of the enzyme is characterized by a high content of dicarboxylic acids. the substrate specificity is studied. leucyl-glycyl-glycin and leucinamide are most intensive in splitting. ... | 1980 | 7385381 |
| [effect of sh-reagents and thiol compounds on aminopeptidase from aspergillus oryzae]. | it is shown that iodacetate and iodacetamide produce an insignificant inhibition of the asp. oryzae aminopeptidase activity, para-chloromercuribenzoate is a stronger inhibitor. dithiotreitol, beta-mercaptoethanol, reduced glutathione also cause a considerable loss in the enzyme activity. the inhibitory effect is intensified with a combined action of para-chloromercuribenzoate and edta. the studies of para-chloromercuribenzoate, iodacetate and iodacetamide effect on the activation of aminopeptida ... | 1980 | 7376280 |
| influence of brinase on fibrinogen: fibrin transition in in vitro and in vivo studies. i. influence of in vitro addition of brinase to plasma on the ethanol gelation test. | the incidence of positive ethanol gelation test (egt) after addition of brinase (a proteolytic enzyme preparation from aspergillus oryzae) to anticoagulated and non-anticoagulated human plasma was studied. in vitro addition of brinase to plasma causes positive egt, and the incidence is dose-dependent. in plasma from warfarin-treated patients and/or after addition of heparin to plasma, prior to the addition of brinase, a significantly reduced incidence of positive egt is observed. the incidence i ... | 1980 | 7351312 |
| [6-phosphogluconate dehydrogenase of aspergillus oryzae (ahlburg). ii. separation and study of isoenzymes]. | through ammonium sulphate fractionation followed by ion-exchange chromatography, two forms of 6-phosphogluconate dehydrogenase (6j-phosphogluconate: nadp+ oxidoreductase e.c. 1.1.1.44) were isolated from mycelium of aspergillus oryzae. the km values for 6pg were the same, but the km for nadp+ was smaller for isoenzyme i. the vmx values were also different, with greater activity for isoenzyme ii. the optimum ph for isoenzyme i was 7.5. whereas the isoenzyme ii had two maximum peaks, at ph 7.5 and ... | 1981 | 7339739 |
| [hydrolysis of n-acetyl-l- and n-acetyl-d,l-methionine by microbial aminacylase]. | the kinetics of n-acetyl-l- and n-acetyl-d,l-methionine hydrolysis by aminoacylase from asp. oryzae were studied. the maximal rate of the reaction was found to be about 2000 mu moles of l-methionine per mg of protein per hour; km was equal to about 1.10(-1) m for both the racemic and optically active substrates. in the presence of co(ii) ions (the molar ratio of n-acetyl-l-methionine/co(ii) was 100:1) the reaction velocity was increased. the values of approximately 4500 and approximately 3000 mu ... | 1981 | 7284482 |
| [localization of secreted enzyme-induced repression of its synthesis by the cells]. | it was shown that under conditions of regulation of secreted enzyme synthesis by its concentration in microorganisms, the repression of synthesis of the enzyme protein polypeptide chain and the concomitant partial repression of labelled amino acid transport into the cells occur. the regulatory effect can be exerted in the absence of transcription as well. these effects are not realized at the post-transcription or post-translation levels. | 1980 | 7248344 |
| [isoenzymes of glucose-6-phosphate dehydrogenase from aspergillus oryzae (ahlburg) (author's transl)]. | two forms (i and ii) of glucose-6-phosphate dehydrogenase (d-glucose-6-phosphate: nadp+ oxidoreductase e.c. 1.1.1.49) were isolated from mycelium of aspergillus oryzae grown on glucose as sole carbon source, through ammonium sulfate fractionation, followed by ion-exchange chromatograhy. the km values for both g6p and nadp+ were very similar, but the vmax was nearly twofold for form ii. the two isoenzymes were inhibited by nadph competitively with nadh+, and the ki value was minimal for isoenzyma ... | 1980 | 7221161 |
| [a case of allergic bronchopulmonary aspergillosis caused by aspergillus oryzae which is used for brewing bean paste (miso) and soy sauce (shoyu) (author's transl)]. | 1982 | 7202073 | |
| dna supercoiling, shortening, and induction of single-strand regions by cis-diamminedichloroplatinum(ii). | cis-diamminedichloroplatinum(ii) was found to bind to covalently closed circular supercoiled, covalently closed circular nonsupercoiled, and single-strand broken relaxed pm2 dna and induce different types of dna conformational changes. using kleinschmidt's technique, it was found that binding of cis-diamminedichloroplatinum(ii) decreased the dna length to 75% of the original single-strand broken relaxed dna without inducing superhelical conformational changes. cis-diamminedichloroplatinum(ii) al ... | 1981 | 7197192 |
| biosynthesis of xyloglucan in suspension-cultured soybean cells. an assay method for xyloglucan xylosyltransferase and attempted synthesis of xyloglucan from udp-d-xylose. | hydrolysis of the total non-cellulosic 14c-polysaccharides from soybean cell wall with carbohydrate hydrolases of an aspergillus oryzae enzyme preparation yielded 14c-monosaccharides and [14c]isoprimeverose (6-o-alpha-d-xylopyranosyl-d-glucopyranose) in which all of the xylosyl residues of the xyloglucan were recovered. based on this finding, an assay method for the activity of xyloglucan xylosyltransferase was developed. when udp-[14c]xylose was incubated with a particulate enzyme fraction from ... | 1981 | 7194336 |
| purification and characterization of 1,2-alpha-mannosidase of aspergillus oryzae. | 1,2-alpha-mannosidase was purified approximately 1,400-fold from an enzyme product of aspergillus oryzae. the enzyme showed a single band in disc gel electrophoresis and the molecular weight was estimated to be about 49,000 daltons by gel exclusion chromatography. the substrate specificity of the enzyme was examined with mannooligosaccharides, yeast mannan, glycopeptides, and a glycoprotein. the alpha-(1 leads to 2)-linking mannose residues located at the nonreducing-ends of the substrates were ... | 1982 | 7118857 |
| delivery of fungal beta-galactosidase to rat brain by means of liposomes. | a significant increase in beta-galactosidase activity was observed in the brain of rats 1 hr after an intravenous injection of liposomes containing beta-galactosidase purified from aspergillus oryzae. the increased activity was proved to have features of the fungal enzyme by differentiating it from rat's native beta-galactosidase in both heat stability and immunochemical studies. blood content of rat brain tissue under the experimental conditions employed was estimated as 0.83% (v/w) from an inf ... | 1982 | 7071841 |
| liquid-chromatographic determination of the total thiamin content of blood. | a liquid-chromatographic method for determining the total thiamin content of blood is presented. blood is deproteinized and incubated with aspergillus oryzae carboxyl proteinase (ec 3.4.23.6; takadiastase) to convert thiamin phosphate esters to free thiamin. an aliquot of the sample is applied to the column (shodex oh-pak m-414) of a high-performance liquid chromatograph. a 100 mg/l solution of potassium ferricyanide in 150 g/l sodium hydroxide is added to the column effluent with a proportionin ... | 1982 | 7055932 |
| a new chromophoric substrate for penicillopepsin and other fungal aspartic proteinases. | the hexapeptide n-alpha-acetylalanylalanyl-lysyl-p- nitrophenylalanylalanylalanylamide has been synthesized and was found to be a good substrate for fungal aspartic proteinases that possess trypsinogen-activating activity, namely penicillopepsin, rhizopus aspartic proteinase, endothia aspartic proteinase and the aspartic proteinases from aspergillus oryzae and penicillium roqueforti. the peptide is rapidly cleaved between the lysine and p-nitrophenylalanine residues. calf chymosin and human reni ... | 1982 | 7052062 |
| [effect of electromagnetic oscillations in the millimeter wave-length range of biological systems]. | 1981 | 7017802 | |
| intracellular localization of two molecular forms of membrane acid protease in aspergillus oryzae. | the intracellular localization of two forms of membrane-bound acid protease (m1 and m2) [ec 3.4.23.6] of aspergillus oryzae (tsujita, y. & endo, a. (1978) eur. j. biochem. 84, 347-353) was investigated. when the mycelia were treated with wall-lytic enzymes, m2 remained in the cells but most of m2 was solubilized and released. the cell wall fraction obtained by mechanical disruption of the mycelia contained less than 5% of the total acid protease activity in the cells. subcellular fractionation o ... | 1980 | 6997281 |
| [semicontinuous cultivation of fungi of the genus aspergillus, producers of hydrolases]. | the production of exohydrolases (alpha-amylase and pectinase) by fungi belonging to the genus aspergillus was studied in the course of batch cultivation and, if immobilized cells were used, in the semicontinuous regime of growth. the cells were immobilized on a fixed filtering plate and on floating, in the growth medium, polyhedrons. such a cultivation of immobilized microbial cells in the semicontinuous regime of growth on submerged polyhedrons freely floating in the nutrient medium makes it po ... | 1982 | 6984129 |
| bilateral endogenous necrotizing scleritis due to aspergillus oryzae. | a case of bilateral necrotizing scleritis due to aspergillus oryzae is reported. the patient was a former addict of intravenous narcotics treated five years previously for meningitis due to the same organism. a seeding focus in the thoracic spine was eventually found. the patient responded well to combined local and systemic therapy with amphotericin b, flucytosine, and natamycin. this represents, to the best of our knowledge, both the first reported case of ocular disease due to this species of ... | 1982 | 6982019 |
| a sensitive radiochemical enzyme assay for s-adenosyl-l-homocysteine and l-homocysteine. | 1981 | 6947702 | |
| effects of fibrinogen fragment and proteolytic enzyme on the immune system in mice. | the immunomodulating ability of fibrinogen fragment (hol-dsk) and proteolytic enzyme with fibrinolytic activity from aspergillus oryzae was analysed in cba/ca, c57bl, c3h/hej and nmri mice. suppressive effects on the blastogenic response to mitogens were noted. no consistent suppressive effect, but sometimes an enhancing one, was recorded on the antibody responses to protein antigens. administration of hol-dsk or proteolytic enzyme shortly after the immunization resulted in a slight inhibition o ... | 1983 | 6848480 |
| effects of phospholipids on substrate specificity of beta-galactosidase purified from aspergillus oryzae. | 1982 | 6807206 | |
| mutagenesis during transformation of bacillus subtilis. i. an increase in "selfing' resulting from hybrid donor dnas. | reverse mutations increase when competent bacillus subtilis cells are transformed with high concentrations of homologous "selfer' dna. a high proportion of the mutants were also transformants of linked genes. a stimulation in the appearance of reversed mutations occurred when homoduplex and heteroduplex "selfer' dnas were used as donors. digestions of native and hybrid dnas with nuclease s1 from aspergillus oryzae resulted in the preferential decrease of mutations as compared to a much smaller i ... | 1981 | 6799809 |
| reversal of fungitoxicity of 8-quinolinols and their copper(ii) bischelates. ii. reversal of the action of 8-quinolinol by dl-alpha-lipoic acid. | the effect of amino acids and derivatives, krebs cycle acids and related compounds, fatty acids, and vitamins and related compounds on the toxicity of 8-quinolinol and bis(8-quinolinolato)copper(ii) to aspergillus oryzae (atcc 1011) was studied. only aliphatic thiol-containing compounds (cysteine, glutathione, dithioerythritol, and dithiothreitol) and dl-alpha-lipoic acid protected against 8-quinolinol but not its copper(ii) bischelate. it is suggested that 8-quinolinol inhibits lipoic acid bios ... | 1981 | 6790147 |
| aminoacylase from aspergillus oryzae. comparison with the pig kidney enzyme. | aminoacylase (ec 3.5.1.14) from aspergillus oryzae was purified from a commercially available crude material by heat treatment, precipitation by polyethyleneimine and ammoniumsulfate, gel chromatography and preparative disc-gel-electrophoresis. the purified product was homogenous as judged by polyacrylamide gel electrophoresis. sds-gel electrophoresis, polyacrylamide-gel-gradient electrohoresis, gel chromatography and amino acid analysis demonstrated the enzyme to be composed of two subunits wit ... | 1980 | 6774495 |
| degradation of amyloid proteins with protease i from aspergillus oryzae. in vivo increase in saa clearance rate after enzyme infusion. | the effect of the thrombolytic enzyme protease i from aspergillus oryzae (brinase) on the amyloid protein aa was investigated. the effect of the enzyme on purified, low molecular weight protein aa was very high. protein aa in intact amyloid fibrils suspended in neutral buffer was also degraded by the enzyme, although at a much lower rate. amyloid fibrils in tissue sections could also be attacked and removed, leaving the tissue structure fairly intact. the in vivo effect of brinase on protein saa ... | 1982 | 6760382 |
| proteinase enzymes relevant to the baking industry. | 1982 | 6754500 | |
| [regulation of alpha-amylase biosynthesis in an aspergillus oryzae 3000 strain based on the principle of feedback from the level of the active enzyme]. | 1983 | 6606299 | |
| [a case of allergic broncho-pulmonary aspergillosis due to asp. oryzae and its subtype]. | 1984 | 6530860 | |
| [aspergillus oryzae as a cause of keratomycosis in the horse]. | a case of a spontaneous mycokeratitis of a previously injured cornea in a horse is described. the infection was caused by aspergillus oryzae. after application of chloramphenicol ophthalmic ointment a corneal clouding was found in the centre which was circularly sharply defined and which - after dispensing dexamethason-neomycin eye drops - expanded all over to a purulent keratitis. the demarcated and initially non purulent mycotic lesions largely improved after the application of tincture of iod ... | 1984 | 6528327 |
| [fatty acid composition of the lipids in fungi of the genus aspergillus developing on mineral media with different carbon sources]. | this work was aimed at studying the effect of different carbon sources in the composition of mineral media on the growth of fungi belonging to the aspergillus genus and on the fatty acid composition of their lipids. a chemically-defined medium with glucose was shown to be optimal for the growth of 18 aspergillus strains and for the synthesis of lipids by them. the fatty acid composition of lipids was studied when the fungi grew in media with different carbon sources. the lipids were shown to con ... | 1984 | 6442390 |
| alteration of the substrate specificity of aspergillus oryzae beta-galactosidase by modification with polyethylene glycol. | beta-galactosidase (beta-d-galactoside galactohydrolase, ec 3.2.1.23) purified from aspergillus oryzae was modified with 2,4,6-trichloro-s-triazine derivatives of polyethylene glycol (activated bpeg) having molecular weights of 600, 1500, 2000, and 4000. polyethylene glycol derivatives were attached to 6 of the 12 amino groups exposed on the surface of the enzyme. upon modification, the enzymatic activity for a water-soluble substrate, o-nitrophenyl beta-d-galactopyranoside, was reduced with inc ... | 1984 | 6436228 |
| effect of glycolipids contained in liposomes on the incorporation of beta-galactosidase into specific organs. | a series of glycolipids were examined to find a system capable of targeting liposomes into specific organs of rats. sulfatide was found to be the best among the components of liposomes examined for delivering the entrapped enzyme, beta-galactosidase from aspergillus oryzae, into the brain and liver; gangliosides, for the spleen; and sphingomyelin, for the lung. to introduce the enzyme into the liver, galactocerebroside was far better than glucocerebroside. these data suggest that the sugar resid ... | 1984 | 6435635 |
| multiple forms of protyrosinase from aspergillus oryzae and their mode of activation at ph 3.0. | this paper describes the isolation of three molecular forms (i-iii) of protyrosinase and catalytically active tyrosinase ( monophenol ,dihydroxyphenylalanine: oxygen oxidoreductase, e.c. 1.14.18.1) from fresh mycelia of aspergillus oryzae bir 128 by (nh4)2so4 fractionation, successive chromatographies and disc-gel electrophoresis. experimental evidence is presented that purified protyrosinase is contaminated with firmly attached proteinases, and that this contamination may account for both the m ... | 1984 | 6424711 |
| energy of binding of aspergillus oryzae beta-glucosidase with the substrate, and the mechanism of its enzymic action. | several beta-d-glucopyranosides (p-nitrophenyl, phenyl, and ethyl), 1-thio-beta-d-glucopyranosides, and phenyl 2-deoxy, 3-deoxy, 4-deoxy, and 6-deoxy beta-d-glucopyranosides were synthesized and used to study the mechanism of the enzymatic action of taka-beta-glucosidase [ec 3.2.1.21 aspergillus oryzae]. kinetic constants of the enzyme for these glycosides were determined from s/v-s or 1/v-1/s plots, and the hydrolysis rates of these compounds with the enzyme, acid (3 n hcl) and alkali (3 n naoh ... | 1983 | 6418735 |
| regulation of the oxidative phase of the pentose phosphate cycle in aspergillus oryzae (ahlburg). i. induction of glucose-6-phosphate dehydrogenase. | the activity of glucose-6-phosphate dehydrogenase changes when the mycelium is grown on different carbon sources. in the presence of ribose, the activity of the enzyme is approximately 3 times higher than when the mycelium is grow in a medium containing glucose. this increase in the enzyme activity is developed progressively when the concentration of ribose increases from 1% to 6% and it is blocked if cycloheximide is added to the medium. this results suggest an increase in "de novo" synthesis o ... | 1983 | 6418104 |
| effects of thiol protease inhibitors on intracellular degradation of exogenous beta-galactosidase in cultured human skin fibroblasts. | the effects of low molecular weight (lmw) protease inhibitors of microbial origin were evaluated on the intracellular degradation of beta-galactosidase purified from aspergillus oryzae and taken up by cultured human skin fibroblasts with beta-galactosidase deficiency. only thiol protease inhibitors showed an effect to increase the enzyme activity. e-64, a specific inhibitor of thiol proteases, prolonged 3-fold a half life of the exogenous beta-galactosidase and when the enzyme was supplied as li ... | 1983 | 6414834 |
| [effect of the physiological conditions on alpha-amylase and glucoamylase formation by a selected strain of aspergillus oryzae]. | the production of alpha-amylase and glucoamylase by a selected strain of aspergillus oryzae was investigated using different carbon and nitrogen sources. the best and most economic fermentation medium for the production of the both amylases in submerged cultures had the following composition (in %): defatted rice brain, 8; corn steep liquor, 3; mgso4 x 7h2o, 0.1; kh2po4, 0.1; cacl2, 0.1. the optimum ph was 5.0. the optimal conditions for biosynthesis of the amylases were as follows: cultivation ... | 1983 | 6413831 |
| [species and organ heterogeneity of arylsulfatases]. | 1984 | 6397752 | |
| effect of drugs on the activity of fungal limit dextrinase & alpha-amylase. | 1983 | 6360862 | |
| [isolation of a specific inhibitor of microbial serine proteinase from kidney bean seeds]. | a protein acting as a specific inhibitor of microbial serine proteinases was isolated from kidney bean seeds. the purification procedure included complex formation between the inhibitor and aspergillus oryzae proteinase. the protein with a mr approximately 10 000 inhibits subtilisin and asp. oryzae proteinase but does not affect trypsin and chymotrypsin. the inhibitor molecule contains no half-cystine residues. | 1983 | 6357292 |
| [affinity chromatography of aminopeptidases]. | the recent data are generalized concerning a series of synthetic oligopeptides which are competitive inhibitors of aminopeptidases of animal, plant and microbic origin. a method for biospecific chromatography of these enzymes is developed, using as ligands such inhibitors as diazo derivatives of p-aminophenyl-, chloromethyl- and methylketones of l-amino acids and peptides, amino acids, aliphatic acid amides. it is established that the most effective inhibitors of aminopeptidases contain l-amino ... | 1983 | 6356542 |
| [sedimentation-biochemical method of determination of sedimentation constants and molecular masses of enzymes]. | the sedimentation-biochemical method of determination of enzymes' sedimentation coefficients and estimation of their molecular masses based on the tiselius' transport technique is discussed. the method permits to determine the sedimentation coefficients of enzyme in the starting mixtures without their preliminary purification. the use of different substrats permits to carry out the studying of several enzymes simultaneously. the frameworks of the method are discussed. | 1983 | 6353201 |
| endonuclease s1-sensitive site in chicken pro-alpha 2(i) collagen 5' flanking gene region. | a site that is preferentially cleaved by the single-strand-specific endonuclease from aspergillus oryzae was located in vitro 180 base pairs upstream from the 5' end of the chicken pro-alpha 2(i) collagen gene. it is found in supercoiled plasmids with a negative superhelical density of -0.024 or more but not in linear dna molecules. the nuclease s1 sensitivity is retained in plasmids containing genomic fragments extending from position +8 to -285 (where +1 is the first transcribed base) and from ... | 1984 | 6324210 |
| secondary structure specificity of the nuclease activity of the 1,10-phenanthroline-copper complex. | the artificial dnase activity of the 1,10-phenanthroline-cuprous ion complex [(op)2cu+] and h2o2 cleaves the a, b, and z forms of dna at different rates. the b structure, formed by most dnas including poly(da-dt) and poly(da) x poly(dt), is the most susceptible to cleavage. it is completely degraded within 1 min by 40 microm 1,10-phenanthroline/4 microm cu2+/7 mm h2o2/7 mm 3-mercaptopropionic acid. the a structure, formed by rna x dna hybrids such as poly(ra) x poly(dt), is cleaved in both stran ... | 1984 | 6320169 |
| stereochemical course of dna hydrolysis by nuclease s1. | nuclease s1 hydrolyzes the sp-diastereomer of 5'-o-(2'-deoxyadenosyl)-3'-o-thymidyl phosphorothioate in h2(18)o to [18o]deoxyadenosine 5'-o-phosphorothioate which can be phosphorylated enzymatically to the sp-diastereomer of [alpha-18o]deoxyadenosine 5'-o-(1-thiotriphosphate). 31p nmr spectroscopy shows the oxygen-18 in this compound to be in a nonbridging position at the alpha-phosphorus, indicating that the hydrolysis reaction catalyzed by nuclease s1 proceeds with inversion of configuration a ... | 1983 | 6296110 |
| secondary structure of bombyx mori and dictyostelium discoideum 5s rrna from s1 nuclease and cobra venom ribonuclease susceptibility, and computer assisted analysis. | the 5s rrnas from bombyx mori and dictyostelium discoideum were end-labeled with [32-p] at either the 5' or 3' end and sequenced using enzymatic digestion. the secondary structure of these molecules was studied using the single-strand specific s1 nuclease and the base-pair specific cobra venom ribonuclease. computer analysis of these results was performed and was used to generate a consensus secondary structure for each molecule. a comparison of these results with those of other workers is prese ... | 1982 | 6278426 |
| s1 nuclease of aspergillus oryzae: characterization of the associated phosphomonoesterase activity. | 1981 | 6272646 | |
| [isolation and characteristics of highly purified s1-nuclease from amylorizin p10x]. | s1-nuclease was purified from the soviet commercial enzyme amylorizin p10x prepared from the surface culture of aspergillus oryzae. the enzyme yield was 33% of total activity. the molecular weight of the enzyme measured by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate was equal to 30,000. the enzyme showed high specificity to single-stranded dna. | 1981 | 6262745 |
| s1-sensitive sites in dna after gamma-irradiation. | dna from gamma-irradiated t1 bacteriophages was analyzed for "single-stranded" sites by cleavage with s1 nuclease from aspergillus oryzae as lesion probe. the ratio of "s1-sensitive sites" to the amount of radiation-induced single-strand breaks was about one. presumably these "denatured" sites were associated with single-strand breaks. the subsequent check for the persistence of "single-stranded" sites within the dna molecule by thermokinetics demonstrated a strong affinity of the nuclease to it ... | 1981 | 6260190 |
| the inverted repeat as a recognizable structural feature in supercoiled dna molecules. | the single-strand-specific endonuclease s1 from aspergillus oryzae introduces highly selective cleavages into supercoiled covalently closed circular dna molecules, but not into their previously linearized counterparts. the cleavage sites are inverted repeats of unit length between 9 and 13 base pairs, separated by a nonrepetitious 2-6 base pairs. such regions may adopt hairpin or similar structures stabilized by the negative superhelix density and may constitute recognition sites for cellular pr ... | 1980 | 6256738 |
| s1 nuclease of aspergillus oryzae: a glycoprotein with an associated nucleotidase activity. | 1980 | 6252849 | |
| crystallization of a complex between ribonuclease t1 and 2'-guanylic acid. | ribonuclease t1 was crystallized under various conditions. form i crystals were produced by microdialysis against 53% (v/v) 2-methyl-2,4-pentanediol in 0.01 m sodium acetate, 0.05% 2'-guanylic acid (2'gmp) and 0.02% nan3 (ph 6.2-7.2). these crystals are tetragonal, space group p41212 and contain two molecules per asymmetric unit; cell dimensions are a = b = 5.86 nm, c = 13.28 nm. form iia and form iib crystals were obtained by microdialysis from a buffer of 0.01-0.05 m sodium acetate, 0.25-0.5% ... | 1980 | 6250834 |
| purification and properties of s1 nuclease from aspergillus. | 1980 | 6246349 | |
| molecular mechanisms involved in the production of chromosomal aberrations. ii. utilization of neurospora endonuclease for the study of aberration production by x-rays in g1 and g2 stages of the cell cycle. | chinese hamster ovary cells (cho) were x-irradiated in g1 and g2 stages of the cell cycle and subsequently neurospora endonuclease (ne) (e.c.3.1.4), an enzyme which is specific in cleaving single-stranded dna, was introduced into the cells, after making the cells permeable by treatment with inactivated sendai virus. with this treatment all classes of x-ray-induced chromatid aberrations increased in g2 cells, whereas in g1 cells an increase in chromosome type of aberrations was found, associated ... | 1980 | 6244487 |
| a chicken repetitive dna sequence that is highly sensitive to single-strand specific endonucleases. | a dna sequence consisting of the 5-mer agagg repeated tandemly 32 times has been detected in a chicken genomic clone and found to be present in about 2000 copies per chicken genome. this sequence was highly susceptible to single-strand specific endonucleases isolated from aspergillus oryzae (s1) and mung bean, but cleavage by a single-strand specific endonuclease isolated from neurospora crassa occurred only at a ph below 5.5. endonucleolytic cutting of the agagg sequence by the single-strand sp ... | 1983 | 6231528 |
| the induction of alpha-amylase by starch in aspergillus oryzae: evidence for controlled mrna expression. | the induction of alpha-amylase by starch has been studied in the filamentous fungus aspergillus oryzae. low levels of alpha-amylase activity were found in both intracellular and extracellular samples from glucose-grown cultures. however, alpha-amylase activity increased when starch was the sole carbon source. the intracellular enzyme activity was induced by a factor of approximately 6.5, while the extracellular activity increased 20-fold over that found in the glucose-grown cultures. regardless ... | 1984 | 6208985 |
| present-day studies on cereals protein nature alpha-amylase inhibitors. | 1983 | 6190085 | |
| kinetic study on chemical modification of taka-amylase a. ii. ethoxycarbonylation of histidine residues. | the modification of taka-amylase a (taa) [ec 3.2.1.1] of aspergillus oryzae by diethylpyrocarbonate (dep) was carried out at 25 degrees c and at ph 5.8 (0.1 m acetate buffer). two out of the six histidine residues were modified with 4.6 mm dep, and two or three histidine residues were modified with 23 mm dep. in both cases, one of them was protected from modification by the presence of 15% maltose. the results suggest that two or three out of the six histidine residues are exposed on the surface ... | 1982 | 6185471 |
| [degradation of inactivated alpha-amylase by associated proteases]. | alpha-amylase preparations often contain small quantities of proteolytic activity which are difficult to remove. on the example of fungal alpha-amylase, such associated proteases have been shown to possess a specific activity to the denatured amylase molecules. the amylase is not attacked under native conditions, whereas in the thermal denaturation a rapid degradation of only the inactivated molecules occurs. a specific metabolic function of these associated proteases in the return of denatured ... | 1982 | 6183852 |
| [effect of glucose on the induced biosynthesis of alpha-amylase in an aspergillus oryzae 3000 strain]. | 1980 | 6176100 | |
| alpha-amylase inhibitor from fungus cladosporium herbarum f-828. | a strain of fungus cladosporium herbarum extracellularly produced an inhibitor specific for mammalian alpha-amylase. the inhibitor was purified 81-fold by freeze-thawing, heat treatment, and column chromatography on deae-cellulose, sephadex g-75, deae-sephacel, and bio-gel p-100. an apparent molecular weight of approximately 18,000 was estimated for the inhibitor using bio-gel p-100 filtration. the purified inhibitor preparation was a glycoprotein containing about 10% carbohydrate. the amino aci ... | 1982 | 6174515 |
| the use of taka-diastase in a[3h]poly(a) hybridization assay of oligo(u) sequences in rna. | a reliable assay of uridylate sequences longer than 10 is described. the procedure is based on the hybridization of [3h]poly(a) with poly(u) or oligo(u) sequences in high ionic conditions and a subsequent degradation of single stranded polynucleotides with purified taka-diastase. a 1:2 complex between poly(a) and poly(u) is formed on which on poly(u) strand is digested by taka-diastase. the procedure is especially suitable for the detection and quantitation of un (n greater than 10) in rna prepa ... | 1981 | 6168676 |
| confirmation of molecular weight of aspergillus oryzae alpha-amylase using the low angle laser light scattering technique in combination with high pressure silica gel chromatography. | molecular weight of aspergillus oryzae alpha-amylase (taka-amylase a) was estimated to be 51,000 +/- 500 by the combined use of high pressure silica gel (tsk-gel g3000sw) chromatography and the low angle laser light scattering technique. the study was carried out partly to assess the performance of the combined technique, and results obtained indicate that it is highly promising as a method to determined protein molecular weight both accurately and quickly. | 1981 | 6165712 |
| molecular structure of taka-amylase a. i. backbone chain folding at 3 a resolution. | the crystal structure of taka-amylase a was studied by an x-ray diffraction method at 3 a resolution. a total of 452 amino acid residues were found from the electron density map at the present stage. the four disulfide bonds and the branched carbohydrate were also located on the map. the difference electron density map of the maltotriose-soaked crystal showed that a maltose unit was bound in the active center left. the binding of iodine atoms to the enzyme was also studied. | 1980 | 6156152 |
| [properties of the amylolytic enzymes in the drug preparation, orase]. | 1980 | 6155292 | |
| s1 nuclease of aspergillus oryzae. | s1 nuclease has found many uses in the analysis of the structure of nucleic acids, and more new applications, such as the mapping of splice points of early mrnas in sv40, will undoubtedly be found. | 1981 | 6101052 |
| antifungal properties of 2-n-alkyn-1-ols. | fourteen 2-n-alkynols (c3-c14, c16, and c18) were tested against aspergillus oryzae, aspergillus niger, trichoderma viride, and myrothecium verrucaria in sabouraud dextrose agar at ph 5.6 and 7.0. toxicity to candida albicans, candida tropicalis, trichophyton mentagrophytes, and mucor mucedo was determined in the same medium at ph 5.6 and 7.0 in the absence and presence of 10% beef serum. fungitoxicity was strongly influenced by chain length, slightly by the ph of the medium, and significantly b ... | 1984 | 6098642 |
| multiple attach hypothesis of alpha-amylase action: action of porcine pancreatic, human salivary, and aspergillus oryzae alpha-amylases. | 1967 | 6076229 | |
| studies on glucose dehydrogenase of aspergillus oryzae. iv. histidyl residue as an active site. | 1967 | 6066288 | |
| studies on glucose dehydrogenase of aspergillus oryzae. 3. general enzymatic properties. | 1967 | 6066287 | |
| studies on glucose dehydrogenase of aspergillus oryzae. ii. purification and physical and chemical properties. | 1967 | 6034674 | |
| [isolation of active protease from aspergillus oryzae culture liquid]. | 1966 | 5999942 | |
| [specificity properties of a protease from aspergillus oryzae]. | 1966 | 5983458 | |
| trehalase in conidia of aspergillus oryzae. | horikoshi, koki (the institute of physical and chemical research, bunkyo-ku, tokyo, japan), and yonosuke ikeda. trehalase in conidia of aspergillus oryzae. j. bacteriol. 91:1883-1887. 1966.-trehalases (soluble trehalase and coat-bound trehalase) were found in the conidia of aspergillus oryzae, and the total activity of the trehalases increased during the germination process. the soluble trehalase was purified by diethylaminoethyl-cellulose column chromatography; its optimal ph, michaelis constan ... | 1966 | 5937243 |
| systemic toxicity of ca-7 (fibrinolytic enzyme from aspergillus oryzae) in the dog. | 1966 | 5925997 | |
| thrombolytic therapy with local perfusions of ca-7 (fibrinolytic enzyme from aspergillus oryzae) in the dog. | 1966 | 5920107 | |
| an experimental method for quantitative evaluation of thrombolysis: effect of ca-7, a protease from aspergillus oryzae. | 1966 | 5920106 | |
| the effect of an enzyme preparation from aspergillus oryzae on the aggregation of rabbit platelets in vitro. | 1966 | 5918678 | |
| interdependence of inoculum size, method of cultivation and substrate composition in amylase production and growth of aspergillus oryzae. | 1965 | 5898323 | |
| [the role of the calcium ion in the maintenance of conformation and active center of alpha-amylase in aspergillus oryzae]. | 1965 | 5888197 | |
| [the role of hydrogen bonds in the maintenance of the conformation and formation of the enzyme-substrate complex of alpha-amylase of aspergillus oryzae]. | 1965 | 5881177 | |
| isolation and characterization of ribosomes from dormant and germinating conidia of aspergillus oryzae. | 1965 | 5880200 | |
| [morphological, physiological, and biochemical studies of aspergillus oryzae variants obtained under the influence of ultraviolet rays]. | 1965 | 5868973 | |
| carbon dioxide fixation in aspergillus oryzae conidia at the initial phase of germination. | 1965 | 5866275 |