Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| succession of phenotypic, genotypic, and metabolic community characteristics during in vitro bioslurry treatment of polycyclic aromatic hydrocarbon-contaminated sediments. | dredged harbor sediment contaminated with polycyclic aromatic hydrocarbons (pahs) was removed from the milwaukee confined disposal facility and examined for in situ biodegradative capacity. molecular techniques were used to determine the successional characteristics of the indigenous microbiota during a 4-month bioslurry evaluation. ester-linked phospholipid fatty acids (plfa), multiplex pcr of targeted genes, and radiorespirometry techniques were used to define in situ microbial phenotypic, gen ... | 2001 | 11282603 |
| long-range (1)h-(15)n heteronuclear shift correlation at natural abundance: a tool to study benzothiazole biodegradation by two rhodococcus strains. | the biodegradation of benzothiazole and 2-hydroxybenzothiazole by two strains of rhodococcus was monitored by reversed phase high-pressure liquid chromatography and by (1)h nuclear magnetic resonance (nmr). both xenobiotics were biotransformed into a hydroxylated derivative of 2-hydroxybenzothiazole by these two strains. the chemical structure of this metabolite was determined by a new nmr methodology: long-range (1)h-(15)n heteronuclear shift correlation without any previous (15)n enrichment of ... | 2001 | 11282584 |
| the desymmetrization of bicyclic beta -diketones by an enzymatic retro-claisen reaction. a new reaction of the crotonase superfamily. | the enzyme 6-oxocamphor hydrolase, which catalyzes the desymmetrization of 6-oxocamphor to yield (2r,4s)-alpha-campholinic acid, has been purified with a factor of 35.7 from a wild type strain of rhodococcus sp. ncimb 9784 grown on (1r)-(+)-camphor as the sole carbon source. the enzyme has a subunit molecular mass of 28,488 da by electrospray mass spectrometry and a native molecular mass of approximately 83,000 da indicating that the active protein is trimeric. the specific activity was determin ... | 2001 | 11278926 |
| the bactericidal activities of hmr 3004, hmr 3647 and erythromycin against gram-positive bacilli and development of resistance. | the bactericidal activities of two new ketolides, hmr 3004 and hmr 3647, and the potential to develop resistance to these two antibiotics were studied in gram-positive bacilli. as judged by time-kill kinetics both ketolides were mostly bacteriostatic, being bactericidal against only highly susceptible isolates of corynebacterium striatum (two isolates) and corynebacterium minutissimum (one isolate). spontaneous resistant mutants were detected in seven of 30 strains tested, mainly in rhodococcus ... | 1999 | 11252337 |
| the fas locus of the phytopathogen rhodococcus fascians affects mitosis of tobacco by-2 cells. | the effect of rhodococcus fascians, the causal agent of leafy gall disease, on the mitotic behavior of synchronized tobacco bright yellow-2 (by-2) cells was investigated. incubation of aphidicolin-synchronized by-2 cells with r. fascians cells specifically resulted in a broader mitotic index peak, an effect that was linked to an intact and expressed fas virulence locus. the obtained results pointed towards an effect of r. fascians on the prophase of mitosis. the relevance of these results to the ... | 2001 | 11248250 |
| isolation of virulent rhodococcus equi from native japanese horses. | r. equi was isolated from soil samples obtained from the environment of seven native japanese horse breeds (hokkaido, kiso, noma, misaki, tokara, miyako and yonaguni) and from fecal samples collected from three native horse breeds (hokkaido, kiso and misaki). virulent r. equi at various levels (ranging from 0.5 to 12.9%) was isolated from the feces or soil environment of hokkaido, kiso and misaki horses. isolates were investigated both for the presence of 15- to 17-kda antigens (virulence-associ ... | 2001 | 11247045 |
| chronic scalp wound infection due to rhodococcus equi in an immunocompetent patient. | a case of chronic scalp wound infection due to rhodococcus equi in an immunocompetent individual following heavily contaminated traumatic injury was successfully treated by combining antibiotics and surgery. the agents used were erythromycin and rifampicin. | 2001 | 11243757 |
| [causes of para-allergy to tuberculin]. | experiments on 56 rabbits infected with microorganisms of the genera mycobacterium, nocardia and rhodococcus revealed that in response to the action of different antigens used t and b systems of immunity induced synthesis of antibodies reactive with nonspecific antigens (mycobacterial antigens, tuberculin). in some cases the statistically significant correlation between the dynamics of blast transformation and the specific lysis of lymphocytes in animals with nocardia and rhodococcus infections ... | 2001 | 11236505 |
| [cavitated pulmonary condensation by rhodococcus equi, in an hiv seropositive individual]. | the first clinical case described in portugal of a human infection by rhodococcus equi in a 35 year-old hiv1 seropositive i.v. drug user is presented. the patient was admitted to hospital due to clinical and radiological suspicion of tuberculosis. while in hospital, a rhodococcus equi cavitated lung infection was diagnosed. by applying a triple antibiotic therapy with erythromycin, rifampin and teicoplanin along with surgical excision of the lower lobe of the right lung, we were able to clinical ... | 2000 | 11234501 |
| detection of genes for alkane and naphthalene catabolism in rhodococcus sp. strain 1bn. | rhodococcus sp. 1bn was isolated from a contaminated site and showed various biodegradative capabilities. besides naphthalene, strain 1bn degraded medium- (c6) and long-chain alkanes (c16-c28), benzene and toluene, alone or when the hydrocarbons were mixed in equal proportions. the nucleotide sequence of an alk polymerase chain reaction (pcr) fragment revealed a 59% nucleotide homology to the pseudomonas oleovorans alkb gene. the nar fragments were highly homologous to genes coding for large and ... | 2000 | 11233165 |
| purification, characterization, and overexpression of flavin reductase involved in dibenzothiophene desulfurization by rhodococcus erythropolis d-1. | the dibenzothiophene (dbt)-desulfurizing bacterium, rhodococcus erythropolis d-1, removes sulfur from dbt to form 2-hydroxybiphenyl using four enzymes, dszc, dsza, dszb, and flavin reductase. in this study, we purified and characterized the flavin reductase from r. erythropolis d-1 grown in a medium containing dbt as the sole source of sulfur. it is conceivable that the enzyme is essential for two monooxygenase (dszc and dsza) reactions in vivo. the purified flavin reductase contains no chromoge ... | 2001 | 11229908 |
| analysis of bacterial community structure in sulfurous-oil-containing soils and detection of species carrying dibenzothiophene desulfurization (dsz) genes. | the selective effects of sulfur-containing hydrocarbons, with respect to changes in bacterial community structure and selection of desulfurizing organisms and genes, were studied in soil. samples taken from a polluted field soil (a) along a concentration gradient of sulfurous oil and from soil microcosms treated with dibenzothiophene (dbt)-containing petroleum (fsl soil) were analyzed. analyses included plate counts of total bacteria and of dbt utilizers, molecular community profiling via soil d ... | 2001 | 11229891 |
| bacterial sources and sinks of isoprene, a reactive atmospheric hydrocarbon. | 2000 | 11220299 | |
| enhancing desulphurization by engineering a flavin reductase-encoding gene cassette in recombinant biocatalysts. | biological desulphurization of petroleum feedstocks and products may offer an attractive alternative to reduce sulphur oxide emissions that cause serious environmental pollution. dibenzothiophene (dbt) desulphurization via the dsz pathway of rhodococcus erythropolis igts8 is an energetically expensive process that consumes reducing equivalents. we have shown in this work that the hpac oxidoreductase from escherichia coli w is able to supply the required fmnh2 to the dsz monooxygenases. the cloni ... | 2000 | 11214801 |
| a novel assay method for glycosphingolipid deacylase by enzyme-linked immunochemical detection of lysoglycosphingolipid. | lysoglycosphingolipids consist of a sphingoid long-chain base and monosaccharide or complex sugar, and they lack the fatty acyl group present in native glycosphingolipids. less than 1 pmol of lyso-forssman glycolipid and lysoganglioside gm1 were detected on a thin-layer chromatogram by an enzyme-linked immunochemical coloration method with anti-forssman glycolipid antibody (fom-1) and cholera toxin b subunit, respectively. each spot between 1 and 100 pmol lyso-forssman glycolipid was immunostain ... | 2001 | 11214737 |
| a taxonomic note on the authorship and date of valid publication of rhodococcus sputi. | authorship of the name rhodococcus sputi is variously attributed to tsukamura 1978 or tsukamura and yano 1985. dna-dna binding data indicate that this species and rhodococcus obuensis tsukamura 1983 and rhodococcus chubuensis tsukamura 1983 are subjective (heterotypic) synonyms. although these organisms have been placed in the genus gordonia as gordonia sputi, the correct name of the taxon created by unification of these three species is directly affected by the date of valid publication of thes ... | 2001 | 11211266 |
| de novo cortical cell division triggered by the phytopathogen rhodococcus fascians in tobacco. | plant growth, development, and morphology can be affected by several environmental stimuli and by specific interactions with phytopathogens. in many cases, plants respond to pathogenic stimuli by adapting their hormone levels. here, the interaction between the phytopathogen rhodococcus fascians and one of its host plants, tobacco, was analyzed phenotypically and molecularly. to elucidate the basis of the cell division modulation and shoot primordia initiation caused by r. fascians, tobacco plant ... | 2001 | 11204782 |
| the role of apoptosis in normal ontogenesis and solid human neoplasms. | mammalian cells are capable of committing "active suicide" or apoptosis in response to specialized pathological mechanisms employing a phylogenetically developed intrinsic program of death, triggered by signal transduction through specific receptors. changes in cellular structure such as: 1) condensation of the nuclear (chromatin) and cytoplasmic structures (especially the mitochondria); 2) blebbing of the cell membrane; 3) characteristic swelling of the endoplasmic reticulum; and 4) fragmentati ... | 2000 | 11204498 |
| resident aerobic microbiota of the adult human nasal cavity. | recent evidence strongly suggests that the microbiota of the nasal cavity plays a crucial role in determining the reaction patterns of the mucosal and systemic immune system. however, little is known about the normal microbiota of the nasal cavity. the purpose of this study was to determine the microbiota in different parts of the nasal cavity and to develop and evaluate methods for this purpose. samples were collected from 10 healthy adults by nasal washes and by swabbing of the mucosa through ... | 2000 | 11200821 |
| rhodococcus equi infections in immunocompetent hosts: case report and review. | rhodococcus equi is an unusual cause of infection in humans. infection in immunocompetent patients is extremely rare-only 19 cases in immunocompetent hosts have been reported. localized infections represent nearly 50% of reported cases. pulmonary infections account for only 42% of infections in immunocompetent hosts, compared with 84% of infections in immunocompromised hosts. the mortality rate among immunocompetent patients is approximately 11%, compared with rates of 50%-55% among human immuno ... | 2001 | 11170969 |
| rhodococcus equi and cytomegalovirus pneumonia in a renal transplant patient: diagnosis by fine-needle aspiration biopsy. | rhodococcus equi is a common cause of pneumonia in animals. human infection is rare. increasing number of cases are being reported in immunosuppressed individuals mostly associated with hiv infection, but also in solid organ transplant recipients and leukemia/lymphoma patients. we report on an adult male who developed pneumonia and gastroenteritis 4 mo after receiving a renal transplant. ct scan of the lungs showed a dominant 2.5-cm upper lobe lung mass and smaller bilateral nodules. he underwen ... | 2001 | 11169894 |
| consecutive bacillary angiomatosis and rhodococcus equi bacteremia during acute leukemia: zoonoses may cause fever in neutropenic patients. | 2000 | 11168145 | |
| virulence-associated protein characterisation of rhodococcus equi isolated from bovine lymph nodes. | rhodococcus equi has a low pathogenicity in cattle, but it occasionally causes lymph node granulomas, which are detected at abattoir post mortem inspection, and must be distinguished from tuberculous granulomas. lymph node lesions were detected in 6719 cattle, from a total of 3,263,622 cattle examined post mortem in abattoirs, in the republic of ireland, during 1997 and 1998. histological examination was performed on all lesions, principally for the purpose of identifying animals with tuberculos ... | 2001 | 11165066 |
| structure-activity relationship of mycoloyl glycolipids derived from rhodococcus sp. 4306. | novel mycoloyl glycolipids with short carbon chains were isolated and purified from rhodococcus sp. 4306, a soil origin of actinomycetales. their chemical structures were identified as trehalose 6,6'-dimycolate (tdm), trehalose 6-monomycolate, glucose 6-monomycolate, mannose 6-monomycolate and fructose 6-monomycolate. the length of carbon chains and number of double bonds of mycolic acids were c(34), c(36)and c(38)saturated, monoenoic and dienoic molecular species, which were much shorter than t ... | 2001 | 11162189 |
| comparative specificities of two evolutionarily divergent hydrolases involved in microbial degradation of polychlorinated biphenyls. | 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate (hopda) hydrolase (bphd) is a key determinant in the aerobic transformation of polychlorinated biphenyls (pcbs) by burkholderia sp. strain lb400 (s. y. k. seah, g. labbé, s. nerdinger, m. johnson, v. snieckus, and l. d. eltis, j. biol. chem. 275:15701-15708, 2000). to determine whether this is also true in divergent biphenyl degraders, the homologous hydrolase of rhodococcus globerulus p6, bphd(p6), was hyperexpressed, purified to apparent homogeneity, a ... | 2001 | 11160080 |
| virulence plasmid of rhodococcus equi contains inducible gene family encoding secreted proteins. | rhodococcus equi causes severe pyogranulomatous pneumonia in foals. this facultative intracellular pathogen produces similar lesions in immunocompromised humans, particularly in aids patients. virulent strains of r. equi bear a large plasmid that is required for intracellular survival within macrophages and for virulence in foals and mice. only two plasmid-encoded proteins have been described previously; a 15- to 17-kda surface protein designated virulence-associated protein a (vapa) and an anti ... | 2001 | 11159951 |
| genetic and biochemical characterization of a novel monoterpene epsilon-lactone hydrolase from rhodococcus erythropolis dcl14. | a monoterpene epsilon-lactone hydrolase (mlh) from rhodococcus erythropolis dcl14, catalyzing the ring opening of lactones which are formed during degradation of several monocyclic monoterpenes, including carvone and menthol, was purified to apparent homogeneity. it is a monomeric enzyme of 31 kda that is active with (4r)-4-isopropenyl-7-methyl-2-oxo-oxepanone and (6r)-6-isopropenyl-3-methyl-2-oxo-oxepanone, lactones derived from (4r)-dihydrocarvone, and 7-isopropyl-4-methyl-2-oxo-oxepanone, the ... | 2001 | 11157238 |
| rhodococcus pyridinivorans sp. nov., a pyridine-degrading bacterium. | the taxonomic position of a bacterial strain (pdb9t) that is capable of degrading pyridine was clarified by a polyphasic taxonomic approach using phenotypic, chemotaxonomic and genetic methods. the cells, which are rods and branched filaments during the early growth phase, fragment into short rods or cocci, thereby completing the growth cycle. strain pdb9t was found to have a cell wall of chemotype iv, mk-8(h2) as the predominant menaquinone, mycolic acids with 36-46 carbon atoms and c16:0' c18: ... | 2000 | 11155994 |
| application of solid-phase extraction to the analysis of the isomers generated in biodesulfurization against methylated dibenzothiophenes. | a solid-phase extraction (spe) technique was applied to analyze and characterize the biodesulfurization reactions against asymmetrically methylated dibenzothiophenes (mdbts) such as 1-, 2-, 3- and 4-methyldibenzothiophenes present in fossil fuels. recently, we found that these mdbts are efficiently degraded by the bacterial strain, rhodococcus erythropolis ka2-5-1. separation and concentration of the microbial desulfurization products from each of the mdbts could be carried out with high efficie ... | 2000 | 11153941 |
| resolution of alcohols by cholesterol oxidase fromrrhodococcus erythropolis: lack of enantiospecificity for the steroids. | 2001 | 11135412 | |
| salt-tolerant phenol-degrading microorganisms isolated from amazonian soil samples. | two phenol-degrading microorganisms were isolated from amazonian rain forest soil samples after enrichment in the presence of phenol and a high salt concentration. the yeast candida tropicalis and the bacterium alcaligenes faecoalis were identified using several techniques, including staining, morphological observation and biochemical tests, fatty acid profiles and 16s/18s rrna sequencing. both isolates, a. faecalis and c. tropicalis, were used in phenol degradation assays, with rhodococcus eryt ... | 2000 | 11131025 |
| rhodococcus equi and hiv-1 infection in uganda. | to describe three cases of rhodococcus equi infection in a cohort of hiv-1 infected adults in entebbe, uganda and to compare this to the rates and presentation of tuberculosis in this cohort. | 2000 | 11120609 |
| [creation and use of a liquid substance based on association of petroleum-oxidizing bacteria]. | an association of four bacterial strains with high oil-oxidizing and bioemulsifying activities, psychrophilicity, resistance to chemical pollutants, and lack of pathogenicity was selected from a collection of natural oil-oxidizing microorganisms. a new liquid preparation containing stabilizers and preservatives that maintain the cell viability and oil-oxidizing activity during long-term storage was developed. a field experiment in oil-polluted sod-podzol and clay sand soils demonstrated that thi ... | 2000 | 11116911 |
| [study of the integral toxicity of aqueous media, polluted by petroleum and petroleum products using bacterial tests]. | a biotest kit was used to assess the integral toxicity level of aquatic medium contamination with petroleum and petroleum-based products. the integral toxicity dynamics was also monitored during biodegradation of petroleum and petroleum-based products by an association of petroleum-degrading strains including acinetobacter sp., mycobacterium flavescens, and rhodococcus sp. the following bacterial tests were used: the bioluminescence (bl) test based on photobacterium leiognathi; electro-orientati ... | 2000 | 11116909 |
| purification, characterization and crystallization of enzymes for dibenzothiophene desulfurization. | dszc and dsza, dbt monooxygenase and dbt sulfone monooxygenase, respectively, involved in dibenzothiophene (dbt) desulfurization, were purified to homogeneity from rhodococcus erythropolis d-1. the two enzymes were crystallized and enzymologically characterized. we found a high activity of flavin reductase in the non-dbt-desulfurizing bacterium, paenibacillus polymyxa a-1, which is essential for dszc and a activities, and purified to homogeneity and characterized the enzyme. | 2000 | 11105249 |
| biodesulfurization and the upgrading of petroleum distillates. | biotechnology offers an alternative way to process fossil fuels. there have been several important advances in the elucidation of the mechanisms of biodesulfurization and the development of a biocatalytic desulfurization process. these include a detailed analysis of the rate and extent of desulfurization of real target molecules in a diesel matrix, the directed evolution of rate- and extent-limiting enzymes for better performance and the expression of the genes in alternative hosts. process inno ... | 2000 | 11102787 |
| isolation and characterization of corynebacterium ulcerans from cephalic implants in macaques. | to determine the prevalence of colonization by corynebacterium ulcerans, we cultured samples from the cephalic implant-skin margin and pharynx of 26 rhesus macaques and one pig-tailed macaque. all but one of the samples from the cephalic implants yielded a mixed population of bacteria. c. ulcerans grew from the cephalic implants in 56% and from the pharynx in 3% of the implanted animals. we screened nine of these isolates for diphtheria toxin (dt) and phospholipase d (pld). polymerase chain reac ... | 2000 | 11099137 |
| composition of soil microbial communities enriched on a mixture of aromatic hydrocarbons. | soil contaminated with c5+, which contained benzene (45%, wt/wt), dicyclopentadiene (dcpd) plus cyclopentadiene (together 20%), toluene (6%), styrene (3%), xylenes (2%), naphthalene (2%), and smaller quantities of other compounds, served as the source for isolation of 55 genomically distinct bacteria (standards). use of benzene as a substrate by these bacteria was most widespread (31 of 44 standards tested), followed by toluene (23 of 44), xylenes (14 of 44), styrene (10 of 44), and naphthalene ... | 2000 | 11097903 |
| evaluation of nasotracheal aspiration as a diagnostic tool for rhodococcus equi pneumonia in foals. | the reliability of preparing bacteriological cultures from nasotracheal aspirates of foals routinely in order to diagnose r. equi pneumonia in foals was studied by isolating rhodococcus equi from specimens obtained from 96 foals by nasotracheal aspiration with a silicon catheter. results were compared with specimens obtained from 21 foals by transtracheal aspiration (percutaneous tracheal puncture). these 117 foals showed clinical signs of respiratory tract infection at sampling. r. equi was iso ... | 2000 | 11093633 |
| crystal structure of the haloalkane dehalogenase from sphingomonas paucimobilis ut26. | the haloalkane dehalogenase from sphingomonas paucimobilis ut26 (linb) is the enzyme involved in the degradation of the important environmental pollutant gamma-hexachlorocyclohexane. the enzyme hydrolyzes a broad range of halogenated cyclic and aliphatic compounds. here, we present the 1.58 a crystal structure of linb and the 2.0 a structure of linb with 1,3-propanediol, a product of debromination of 1,3-dibromopropane, in the active site of the enzyme. the enzyme belongs to the alpha/beta hydro ... | 2000 | 11087355 |
| search for virulence-associated antigens of rhodococcus equi in strains isolated from patients with acquired immunodeficiency syndrome. | rhodococcus equi (formerly corynebacterium equi) are known to be highly virulent, intermediate in virulence, or avirulent correlated with specific virulence-associated antigens identified immunochemically by different molecular weights. the association of virulence antigens with infection of aids patients by this organism has not been sufficiently evaluated in brazil or italy. the objective of the present study was to search for virulence-associated antigens of 15-to 17-kd and 20-kd in rhodococc ... | 1999 | 11084666 |
| dna sequence and comparison of virulence plasmids from rhodococcus equi atcc 33701 and 103. | the virulence plasmids of the equine virulent strains rhodococcus equi atcc 33701 and 103 were sequenced, and their genetic structure was analyzed. p33701 was 80,610 bp in length, and p103 was 1 bp shorter; their sequences were virtually identical. the plasmids contained 64 open reading frames (orfs), 22 of which were homologous with genes of known function and 3 of which were homologous with putative genes of unknown function in other species. putative functions were assigned to five orfs based ... | 2000 | 11083803 |
| [cavitating pneumonia in a patient infected with human immunodeficiency virus]. | 2000 | 11075487 | |
| biodegradation of a high molecular weight aliphatic ether--indications of an unusual biodegradation pathway. | an aliphatic ether (1-phytanyl-1-octadecanyl-ether) of high molecular weight was used as a sole carbon source in degradation experiments with different aerobic bacteria. the enriched culture b5, obtained from fuel contaminated soils, was able to degrade the substance for more than 90%. a culture of rhodococcus ruber was similarly effective. detailed investigation of the metabolites allowed us to characterize an unusual degradation pathway via a mid-chain oxidation mechanism ('internal oxidative ... | 1999 | 11068823 |
| clinical application of a polymerase chain reaction assay in the diagnosis of pneumonia caused by rhodococcus equi in a horse. | diagnosis of pneumonia caused by rhodococcus equi can be made more rapidly by use of a polymerase chain reaction (pcr) assay than by use of conventional bacteriologic culture techniques. use of a pcr assay aids in the differentiation between virulent and avirulent strains of r equi, and the assay may be used to identify r equi in feces and soil of breeding farms. | 2000 | 11061388 |
| [rhodococcus equi pneumonia in patients with aids]. | rhodococus equi infection is frequent in animals, but rare in humans. it usually appears as an opportunistic infection, occurring in clinical settings where a compromise of cellular immunity exists, such as in leukemia, neoplastic or transplanted patients. it is currently considered, as another opportunistic aids infection. sixty six cases of rhodococus equi pneumonia have been published until march 1996. the differential diagnosis includes tb and staphyloccocal pneumonia and is generally an inf ... | 2000 | 11059055 |
| relationships between colony morphotypes and oil tolerance in rhodococcus rhodochrous. | a mucoidal strain of rhodococcus rhodochrous was resistant to 10% (vol/vol) n-hexadecane, while its rough derivatives were sensitive. when the extracellular polysaccharide (eps) produced by the mucoidal strain was added to cultures of the rough strains, the rough strains gained resistance to n-hexadecane. thus, eps confer tolerance to n-hexadecane in members of the genus rhodococcus. | 2000 | 11055965 |
| identification of corynebacterium bovis and other coryneforms isolated from bovine mammary glands. | bovine mastitis remains the most economically important disease in dairy cows. corynebacterium bovis, a lipid-requiring corynebacterium spp., is frequently isolated from the milk of infected mammary glands of dairy cows and is associated with reduced milk production. a total of 212 coryneform bacteria isolated from the milk of dairy cows were obtained from mastitis reference laboratories in the united states and canada. all isolates had been presumptively identified as corynebacterium bovis base ... | 2000 | 11049082 |
| [a diagnostic medium for arcanobacterium haemolyticum and other bacterial species reacting with hemolytic synergism to the equi-factor of rhodococcus equi]. | colonies of arcanobacterium haemolyticum on common blood agar can be easily overlooked. therefore a diagnostic medium was developed, on which a. haemolyticum colonies produce a conspicuous zone of complete hemolysis. the medium under question is blood agar prepared from the columbia blood agar base and 5% washed sheep erythrocytes sensitised with equi factor (ef) of rhodococcus equi. optimally, 10 activity units (au) of ef per 1 ml were used. ef was titrated on a non-nutrient medium consisting o ... | 2000 | 11040494 |
| [rhodococcus equi brain abscess in a patient in hemodialysis]. | 2000 | 11039267 | |
| hydrolysis of lysergamide to lysergic acid by rhodococcus equi a4. | from a mixture of lysergamide and its epimer isolysergamide, rhodococcus equi a4 containing amidase preferentially hydrolyzed lysergamide into lysergic acid. | 2001 | 11035188 |
| immunohistochemical detection of virulence-associated rhodococcus equi antigens in pulmonary and intestinal lesions in horses. | two horses with rhodococcus equi infection were examined post mortem by an immunohistochemical method (peroxidase-antiperoxidase; pap) with a monoclonal antibody (mab 10g5) to the 15-17 kda antigen of r. equi. one of the horses was also examined bacteriologically, r. equi being isolated in culture. immunolabelling with this mab was marked and widespread. on the other hand, the immunohistochemical reactivity of infected macrophages with a polyclonal antibody specific for lysozyme was slight. thus ... | 2000 | 11032673 |
| stable isotope incorporation triples the upper mass limit for determination of elemental composition by accurate mass measurement. | by comparing electrospray ionization fourier-transform ion cyclotron resonance (ft-icr) mass spectra and collision-induced dissociation (cid) ft-icr mass spectra of a phospholipid (851 da) extracted from natural abundance and 99% 13c bacterial growth media, we are able to reduce its number of possible elemental compositions (based on +/-10 ppm externally calibrated mass accuracy and biologically relevant compositional constraints) from 394 to 1. the basic idea is simply that the mass of a molecu ... | 2000 | 11014445 |
| microbial degradation of the multiply branched alkane 2,6,10,15,19, 23-hexamethyltetracosane (squalane) by mycobacterium fortuitum and mycobacterium ratisbonense. | among several bacterial species belonging to the general gordonia, mycobacterium, micromonospora, pseudomonas, and rhodococcus, only two mycobacterial isolates, mycobacterium fortuitum strain nf4 and the new isolate mycobacterium ratisbonense strain sd4, which was isolated from a sewage treatment plant, were capable of utilizing the multiply branched hydrocarbon squalane (2,6,10,15,19, 23-hexamethyltetracosane) and its analogous unsaturated hydrocarbon squalene as the sole carbon source for grow ... | 2000 | 11010899 |
| bacterial phosphating of mild (unalloyed) steel. | mild (unalloyed) steel electrodes were incubated in phosphate-buffered cultures of aerobic, biofilm-forming rhodococcus sp. strain c125 and pseudomonas putida mt2. a resulting surface reaction leading to the formation of a corrosion-inhibiting vivianite layer was accompanied by a characteristic electrochemical potential (e) curve. first, e increased slightly due to the interaction of phosphate with the iron oxides covering the steel surface. subsequently, e decreased rapidly and after 1 day reac ... | 2000 | 11010888 |
| [growth of rhodococcus opacus on mixtures of monohalogenated benzenes and phenols]. | the growth of rhodococcus opacus gm-14 on mixtures of 2-chloro- and 2-bromophenol, of 4-chloro, 4-bromo-, and 4-iodophenol, and of chloro-, bromo-, and iodobenzenes was accompanied by consumption of the substrates and excretion of halogen ions to the medium. during the growth on monochlorophenols, the substrates were consumed sequentially in the following order: 4-chloro-, 3-chloro-, and then 2-chlorophenol. chlorine ions were excreted in a two-phase manner in amounts comprising 79% of the theor ... | 2000 | 11008684 |
| the iron dependent regulatory protein ider (dtxr) of rhodococcus equi. | this paper reports the presence of an ider gene, which encodes an iron-dependent regulatory protein, in rhodococcus erythropolis and in the intracellular pathogen rhodococcus equi. the ider gene of the latter encoded a protein of 230 amino acids with a molecular mass of 25619. the alpha-helices forming the helix-turn-helix motif of the r. equi protein were identical to those of the dtxr protein of corynebacterium diphtheriae, which is an ider homologue. this indicates that the two proteins bind ... | 2000 | 11004391 |
| leafy gall formation is controlled by fasr, an arac-type regulatory gene in rhodococcus fascians. | rhodococcus fascians can interact with many plant species and induce the formation of either leafy galls or fasciations. to provoke symptoms, r. fascians strain d188 requires pathogenicity genes that are located on a linear plasmid, pfid188. the fas genes are essential for virulence and constitute an operon that encodes, among other functions, a cytokinin synthase gene. expression of the fas genes is induced by extracts of infected plant tissue only. we have isolated an arac-type regulatory gene ... | 2000 | 11004184 |
| in vitro effects of sterculic acid on lipid biosynthesis in rhodococcus opacus strain pd630 and isolation of mutants defective in fatty acid desaturation. | the in vivo effects of sterculic acid methyl ester on triacylglycerol fatty acid composition in the oleaginous, hydrocarbon-degrading bacterium r. opacus strain pd630 was investigated. sterculic acid, a cyclopropene fatty acid and an inhibitor of the stearoyl-coa desaturase system, strongly inhibited the synthesis of monoenic fatty acids, of saturated fatty acids with more than 16 carbon atoms and of odd-numbered fatty acids when added to the culture medium. in addition, chemical mutagenesis and ... | 2000 | 10981688 |
| cloning and expression of the nitrile hydratase and amidase genes from bacillus sp. br449 into escherichia coli. | a moderate thermophile, bacillus sp. br449 was previously shown to exhibit a high level of nitrile hydratase (nhase) activity when growing on high levels of acrylonitrile at 55 degrees c. in this report, we describe the cloning of a 6.1 kb sali dna fragment encoding the nhase gene cluster of br449 into escherichia coli. nucleotide sequencing revealed six orfs encoding (in order), two unidentified putative proteins, amidase, nhase beta- and alpha-subunits and a small putative protein of 101 amino ... | 2000 | 10978771 |
| effects of prior feeding on pharmacokinetics and estimated bioavailability after oral administration of a single dose of microencapsulated erythromycin base in healthy foals. | to determine effects of prior feeding on pharmacokinetics and estimated bioavailability of orally administered microencapsulated erythromycin base (meb) in healthy foals. | 2000 | 10976728 |
| distribution of alkb genes within n-alkane-degrading bacteria. | fifty-four bacterial strains belonging to 37 species were tested for their ability to assimilate short chain and/or medium chain liquid n-alkanes. a gene probe derived from the alkb gene of pseudomonas oleovorans atcc 29347 was utilized in hybridization experiments. results of southern hybridization of pcr-amplificates were compared with those of colony hybridization and dot blot hybridization. strongest signals were received only from gram-negative bacteria growing solely with short n-alkanes ( ... | 2000 | 10971768 |
| accumulation and mobilization of storage lipids by rhodococcus opacus pd630 and rhodococcus ruber ncimb 40126. | the time course of the accumulation of triacylglycerols (tags) in rhodococcus opacus pd630 or of tags plus polyhydroxyalkanoates (pha) in rhodococcus ruber ncimb 40126 with gluconate or glucose as carbon source, respectively, was studied. in addition, we examined the mobilization of these storage compounds in the absence of a carbon source. r. opacus accumulated tags only after the exhaustion of ammonium in the medium, and, with a fixed concentration of the carbon source, the amounts of tags in ... | 2000 | 10968636 |
| microbiology of the stalactites from grotta dei cervi, porto badisco, italy. | the active stalactites from grotta dei cervi, porto badisco, southeastern italy, were sampled to investigate the microbial communities present in these speleothems. sampling was carried out in a transect about 150 m long in the central gallery, where numerous gram-positive bacteria were isolated. actinomycetes of the genus streptomyces were the most abundant, followed by members of the genus bacillus. further isolates were assigned to the genera amycolatopsis, arthrobacter; agromyces. micrococcu ... | 2000 | 10963330 |
| rapid characterisation of deep-sea actinomycetes for biotechnology screening programmes. | a continual need in natural product discovery is dereplication, that is the ability to exclude previously tested microorganisms from screening programmes. whole-cell fingerprinting techniques offer an ideal solution to this problem because of their rapidity and reproducibility, dependence on small samples, and automation. one such technique, curie-point pyrolysis mass spectrometry (pyms), has been deployed for the characterisation of a unique collection of actinomycetes recovered from pacific oc ... | 2000 | 10959565 |
| [equine rhodococcus equi pneumonia: first report in israel and its significance for man]. | rhodococcus equi is an important pathogen in young horses. in recent years it has been increasingly recognized as an opportunistic infectious agent in patients with immune deficiency. the increase in recognized cases may be related to the increased prevalence of aids. however, more cases may have been recognized lately due to increased awareness of the pathogenicity of this bacterium. based on medical reports, there appears to be an association between rhodococcus equi infections and exposure to ... | 1999 | 10959322 |
| pharmacokinetics of erythromycin estolate and erythromycin phosphate after intragastric administration to healthy foals. | to determine pharmacokinetics and plasma concentrations of erythromycin and related compounds after intragastric administration of erythromycin phosphate and erythromycin estolate to healthy foals. | 2000 | 10951982 |
| microbial conversion of indene to indandiol: a key intermediate in the synthesis of crixivan. | indene is oxidized to mixtures of cis- and trans-indandiols and related metabolites by pseudomonas putida and rhodococcus sp. isolates. indene metabolism is consistent with monooxygenase and dioxygenase activity. p. putida resolves enantiomeric mixtures of cis-1,2-indandiol by further selective oxidation of the 1r, 2s-enantiomer yielding high enantiomeric purity of cis-(1s, 2r)-indandiol, a potential intermediate in the synthesis of indinavir sulfate (crixivan), a protease inhibitor used in the ... | 1999 | 10935755 |
| lung nodular lesions in heart transplant recipients. | to describe the characteristics and etiology of lung nodules after heart transplantation (ht). during a 6-year period 147 patients received ht and 130 survived more than 1 week. nodular lesions were demonstrated after ht in 13 patients (10%). median age was 53 years, and all patients were male. nodules were detected 23 to 158 days after ht (median, 66 days). an etiologic diagnosis was made in all but 1 case: aspergillus (5), nocardia-rhodococcus (4), and cytomegalovirus (cmv) (3). previous sever ... | 2000 | 10930815 |
| propionicin sm1, a bacteriocin from propionibacterium jensenii df1: isolation and characterization of the protein and its gene. | we purified a bacteriocin from the cell-free supernatant of propionibacterium jensenii df1 isolated from swiss raw milk, and named it propionicin sm1. the heat-stable protein was strongly bactericidal against p. jensenii dsm20274. on the basis of the n-terminal amino acid sequence of the purified protein, a degenerate oligonucleotide probe was designed to locate and clone the corresponding gene of p. jensenii df1. it hybridized exclusively with the df1l-resident plasmid plme106, but not with chr ... | 2000 | 10930068 |
| pathogenicity of rhodococcus equi expressing a virulence-associated 20 kda protein (vapb) in foals. | rhodococcus equi strains of intermediate virulence (imv) for mice possess a 20kda protein designated virulence associated protein b (vapb) and a virulence plasmid of 79-100kb, and can be recovered from the submaxillary lymph nodes of pigs. the pathogenicity of such r. equi strains for foals is unknown. in this study, two foals, 42 and 43 days of age, were infected intratracheally with 10(6) and 10(9) cells of r. equi imv strain a5, respectively. the foal infected with 10(9) cells of strain a5 be ... | 2000 | 10925043 |
| multiple respiratory bursts as a response to veratrate stress in rhodococcus erythropolis cells. | although rhodococcus spp. strains are able to degrade methoxyphenols by enzymatic means, the contact with veratric acid (3, 4-dimethoxybenzoic acid, hereafter called veratrate) is very stressful for the cells of rhodococcus erythropolis dsm 1069 (rh). within 5 min of contact veratrate in phosphate buffer, the emergence of many vacuoles was observed in the cell body and respiratory bursts, with violent endogenous oxygen uptake, took place several times during the 24 h incubation. during these pea ... | 2000 | 10924225 |
| rhodococcus l-phenylalanine dehydrogenase: kinetics, mechanism, and structural basis for catalytic specificity. | phenylalanine dehydrogenase catalyzes the reversible, pyridine nucleotide-dependent oxidative deamination of l-phenylalanine to form phenylpyruvate and ammonia. we have characterized the steady-state kinetic behavior of the enzyme from rhodococcus sp. m4 and determined the x-ray crystal structures of the recombinant enzyme in the complexes, e.nadh.l-phenylalanine and e.nad(+). l-3-phenyllactate, to 1.25 and 1.4 a resolution, respectively. initial velocity, product inhibition, and dead-end inhibi ... | 2000 | 10924111 |
| characterization of s-triazine herbicide metabolism by a nocardioides sp. isolated from agricultural soils. | atrazine, a herbicide widely used in corn production, is a frequently detected groundwater contaminant. nine gram-positive bacterial strains able to use this herbicide as a sole source of nitrogen were isolated from four farms in central canada. the strains were divided into two groups based on repetitive extragenic palindromic (rep)-pcr genomic fingerprinting with eric and boxa1r primers. based on 16s ribosomal dna sequence analysis, both groups were identified as nocardioides sp. strains. none ... | 2000 | 10919761 |
| association of disease with isolation and virulence of rhodococcus equi from farm soil and foals with pneumonia. | to determine whether isolation and virulence of rhodococcus equi from soil and infected foals are associated with clinical disease. | 2000 | 10909463 |
| a repeat-batch membrane bioreactor with a phase inversion for the desaturation of isopropyl palmitate by a mutant rhodococcus strain. | a repeat-batch membrane bioreactor was constructed for the novel bioconversion of isopropyl hexadecanoate to isopropyl cis-6-hexadecenoate by a rhodococcus mutant. the addition of glutamate, thiamine, and mgso(4) was very effective in improving not only the rate and yield of the bioconversion but also the maintenance of desaturation activity during cell recycling. an oil-in-water (o/w) type emulsion of the reaction medium was inverted to a water-in-oil (w/o) type by discharging the water phase f ... | 2000 | 10908790 |
| purification, characterization, and cdna cloning of a novel acidic endoglycoceramidase from the jellyfish, cyanea nozakii. | endoglycoceramidase (ec ) is an enzyme capable of cleaving the glycosidic linkage between oligosaccharides and ceramides in various glycosphingolipids. we report here the purification, characterization, and cdna cloning of a novel endoglycoceramidase from the jellyfish, cyanea nozakii. the purified enzyme showed a single protein band estimated to be 51 kda on sds-polyacrylamide gel electrophoresis. the enzyme showed a ph optimum of 3.0 and was activated by triton x-100 and lubrol px but not by s ... | 2000 | 10882727 |
| substrate specificity of regiospecific desaturation of aliphatic compounds by a mutant rhodococcus strain. | substrate specificity of cis-desaturation of alipahtic compounds by resting cells of a mutant, rhodococcus sp. strain ksm-mt66, was examined. among substrates tested, the rhodococcal cells were able to convert n-alkanes (c13-c19), 1-chloroalkanes (c16 and c18), ethyl fatty acids (c14-c17) and alkyl (c1-c4) esters of palmitic acid to their corresponding unsaturated products of cis configuration. the products from n-alkanes and 1-chloroalkanes had a double bond mainly at the 9th carbon from their ... | 2000 | 10879483 |
| molecular analysis of surfactant-driven microbial population shifts in hydrocarbon-contaminated soil. | we analyzed the impact of surfactant addition on hydrocarbon mineralization kinetics and the associated population shifts of hydrocarbon-degrading microorganisms in soil. a mixture of radiolabeled hexadecane and phenanthrene was added to batch soil vessels. witconol sn70 (a nonionic, alcohol ethoxylate) was added in concentrations that bracketed the critical micelle concentration (cmc) in soil (cmc') (determined to be 13 mg g(-1)). addition of the surfactant at a concentration below the cmc' (2 ... | 2000 | 10877792 |
| cloning and expression of gene encoding a novel endoglycoceramidase of rhodococcus sp. strain c9. | endoglycoceramidase (egcase) is an enzyme capable of cleaving the glycosidic linkage between oligosaccharides and ceramides of various glycosphingolipids. we previously reported that the asn-glu-pro (nep) sequence is part of the active site of egcase of rhodococcus sp. strain m-777. this paper describes the molecular cloning of a new egcase gene utilizing the nep sequence from the genomic library of rhodococcus sp. strain c9, which was clearly distinguishable from m-777 by 16s rdna analysis. c9 ... | 2000 | 10876169 |
| degradation of anaerobic reductive dechlorination products of aroclor 1242 by four aerobic bacteria. | we studied the aerobic degradation of eight pcb congeners which comprise from 70 to 85% of the anaerobic dechlorination products from aroclor 1242, including 2-, 4-, 2,4-, 2,6-, 2,2'-, 2,4'-, 2,2', 4-, and 2,4,4'-chlorobiphenyl (cb), and the biodegradation of their mixtures designed to simulate anaerobic dechlorination profiles m and c. strains comamonas testosteroni vp44 and rhodococcus erythreus ny05 preferentially oxidized a para-substituted ring, while rhodococcus sp. rha1, similar to well k ... | 1999 | 10870552 |
| [oxidation of organic compounds by rhodococcus erythropolis 3/89 propanomonooxygenase]. | the ability of propane monooxygenase of rhodococcus erythropolis 3/89 to catalyze oxidation of higher liquid alkenes and aromatic hydrocarbons was studied. optimal conditions of tetradecene epoxidation and benzene hydroxylation were found. under these conditions, oxidation was shown to be accompanied by a 100% conversion of benzene to phenol. | 2000 | 10867943 |
| hiv-infected patient with a rhodococcus equi pneumonia. | the case history of a hiv patient with a pulmonary infect of rhodococcus equi is presented. he recovered after prolonged treatment with antibiotics and lobectomy. the rhodococcus equi infection was the presenting symptom of his impaired immune status caused by hiv infection. | 2000 | 10862999 |
| change in natural history of opportunistic infections in hiv-infected patients. | 2000 | 10862994 | |
| enzymatic dehalogenation of gas phase substrates with haloalkane dehalogenase. | haloalkane dehalogenase is an enzyme capable of catalyzing the conversion of short-chained (c(2)-c(8)) aliphatic halogenated hydrocarbons to a corresponding primary alcohol. because of its broad substrate specificity for mono-, di-, and trisubstituted halogenated hydrocarbons and cofactor independence, haloalkane dehalogenases are attractive biocatalysts for gas-phase bioremediation of pollutant halogenated vapor emissions. a solid preparation of haloalkane dehalogenase from rhodococcus rhodochr ... | 2000 | 10861403 |
| room-temperature ionic liquids as replacements for organic solvents in multiphase bioprocess operations. | organic solvents are widely used in a range of multiphase bioprocess operations including the liquid-liquid extraction of antibiotics and two-phase biotransformation reactions. there are, however, considerable problems associated with the safe handling of these solvents which relate to their toxic and flammable nature. in this work we have shown for the first time that room-temperature ionic liquids, such as 1-butyl-3-methylimi- dazolium hexafluorophosphate, [bmim][pf(6)], can be successfully us ... | 2000 | 10861402 |
| real-time monitoring of nitrile biotransformations by mid-infrared spectroscopy. | in this study mid-infrared spectroscopy was used to follow the enzyme kinetics involved in nitrile biocatalysis using whole cell suspensions of the bacterium rhodococcus rhodochrous ll100-21. the bacteria were grown on acetonitrile to induce a two-step enzymatic pathway. acetonitrile was biotransformed to acetamide by a nitrile hydratase enzyme and subsequently to acetic acid (carboxylate ion) by an amidase enzyme. the bacteria were also grown on benzonitrile to induce a one-step enzymatic pathw ... | 2000 | 10856779 |
| desulfurization of alkylated forms of both dibenzothiophene and benzothiophene by a single bacterial strain. | thirty-five bacterial strains capable of converting dibenzothiophene into 2-hydroxybiphenyl were isolated. among them rhodococcus erythropolis ka2-5-1 was chosen for further characterization because of its ability to retain high desulfurization activity stably. pcr cloning and dna sequencing of a ka2-5-1 genomic dna fragment showed that it was practically identical with dszabc genes from rhodococcus sp. igts8, a representative carbon-sulfur-bond-targeted dibenzothiophene-degrading bacterium. ka2 ... | 2000 | 10856644 |
| post-translational modification is essential for catalytic activity of nitrile hydratase. | nitrile hydratase from rhodococcus sp. n-771 is an alphabeta heterodimer with a nonheme ferric iron in the catalytic center. in the catalytic center, alphacys112 and alphacys114 are modified to a cysteine sulfinic acid (cys-so2h) and a cysteine sulfenic acid (cys-soh), respectively. to understand the function and the biogenic mechanism of these modified residues, we reconstituted the nitrile hydratase from recombinant unmodified subunits. the alphabeta complex reconstituted under argon exhibited ... | 2000 | 10850812 |
| [molecular biology of enzymes degrading pollutants: diversity and convergent evolution observed on extradiol dioxygenases]. | 2000 | 10846472 | |
| rhodococcus koreensis sp. nov., a 2,4-dinitrophenol-degrading bacterium. | a 2,4-dinitrophenol-degrading bacterial strain, dnp505t, which was isolated from an industrial wastewater, was taxonomically studied by a polyphasic approach using phenotypic, chemotaxonomic and genetic methods. strain dnp505t has a cell wall of chemotype iv containing meso-diaminopimelic acid, arabinose and galactose. the predominant menaquinone is mk-8(h2). mycolic acids contain 43-53 carbon atoms. strain dnp505t has a cellular fatty acid profile containing straight-chain saturated, unsaturate ... | 2000 | 10843063 |
| detection of the 20-kda virulence-associated antigen of rhodococcus equi in malakoplakia-like lesion in pleural tissue obtained from an aids patient. | a malakoplakia-like lesion was detected in a pleural biopsy from an aids patient presenting clinical and radiologic features of pneumonia. cultures of bronchoalveolar lavage and pleural fluid evidenced rhodococcus equi as the causative agent of pleuro-pulmonary infection. immunochemical characterization of the r. equi isolate showed the presence of a strain similar to the atcc 33704 reference strain presenting the capsular antigen of serotype 4, and the intermediate virulence-associated antigen ... | 2000 | 10834389 |
| rhodococcus opacus strain pd630 as a new source of high-value single-cell oil? isolation and characterization of triacylglycerols and other storage lipids. | the triacylglycerol (tag)-accumulating, hydrocarbon-degrading bacterium rhodococcus opacus strain pd630 and chemically induced storage-deficient mutants derived from this strain were investigated for their capability to accumulate storage lipids in the cytoplasm during cultivation under nitrogen-limiting conditions. acylglycerols were analysed by matrix-associated laser desorption ionization-time of flight mass spectrometry (maldi-tof) and by reversed-phase hplc. fatty acids comprising 13-19 car ... | 2000 | 10832641 |
| metabolism of carveol and dihydrocarveol in rhodococcus erythropolis dcl14. | rhodococcus erythropolis dcl14 assimilates all stereoisomers of carveol and dihydrocarveol as sole source of carbon and energy. induction experiments with carveol- or dihydrocarveol-grown cells showed high oxygen consumption rates with these two compounds and with carvone and dihydrocarvone. (dihydro)carveol-grown cells of r. erythropolis dcl14 contained the following enzymic activities involved in the carveol and dihydrocarveol degradation pathways of this micro-organism: (dihydro)carveol dehyd ... | 2000 | 10832640 |
| methods for intense aeration, growth, storage, and replication of bacterial strains in microtiter plates. | miniaturized growth systems for heterogeneous culture collections are not only attractive in reducing demands for incubation space and medium but also in making the parallel handling of large numbers of strains more practicable. we report here on the optimization of oxygen transfer rates in deep-well microtiter plates and the development of a replication system allowing the simultaneous and reproducible sampling of 96 frozen glycerol stock cultures while the remaining culture volume remains froz ... | 2000 | 10831450 |
| cholesterol oxidase: sources, physical properties and analytical applications. | since flegg (h.m. flegg, an investigation of the determination of serum cholesterol by an enzymatic method, ann. clin. biochem. 10 (1973) 79-84) and richmond (w. richmond, the development of an enzymatic technique for the assay of cholesterol in biological fluids, scand. j. clin. lab. invest. 29 (1972) 25; w. richmond, preparation and properties of a bacterial cholesterol oxidase from nocardia sp. and its application to enzyme assay of total cholesterol in serum, clinical chemistry 19 (1973) 135 ... | 2000 | 10822008 |
| efficient production of desulfurizing cells with the aid of expert system. | an expert system was used to achieve the high production of desulfurizing cells of rhodococcus erythropolis ka 2-5-1. by adding a proper amount of sulfur containing component with the aid of the expert system, we could avoid excess feeding which resulted in the lowering of desulfurizing activity and starvation which caused serious damage to cell growth. in order to determine the addition amount by the expert system, the data of the amount of chemical elements contained in the cells were used as ... | 2000 | 10817820 |
| cooperation between reactive oxygen and nitrogen intermediates in killing of rhodococcus equi by activated macrophages. | rhodococcus equi is a facultative intracellular bacterium of macrophages which can infect immunocompromised humans and young horses. in the present study, we examine the mechanism of host defense against r. equi by using a murine model. we show that bacterial killing is dependent upon the presence of gamma interferon (ifn-gamma), which activates macrophages to produce reactive nitrogen and oxygen intermediates. these two radicals combine to form peroxynitrite (onoo(-)), which kills r. equi. mice ... | 2000 | 10816516 |
| [effect of composition of cellular lipids of formation of nonspecific antibiotic resistance of alkanotrophic rhodocoocci]. | the antibiotic resistance and lipid composition of rhodococci grown in rich organic media with gaseous or liquid n-alkanes were studied. hydrocarbon-grown rhodococci exhibited an increased resistance to a wide range of antibiotics (aminoglycosides, linkosamides, macrolides, beta-lactams, and aromatic compounds). the enhanced antibiotic resistance of rhodococci grown on n-alkanes correlated with an increased content of total cell lipids (up to 14-28%) and saturated straight-chain fatty acids (c16 ... | 2000 | 10808491 |
| [dependence of transformation of chlorophenols by rhodococci on position and number of chlorine atoms in the aromatic ring]. | study of the conversion of chlorophenols by rhodococcus opacus 1g, r. rhodnii 135, r. rhodochrous 89, and r. opacus 1cp disclosed the dependence of the conversion rate and pathway on the number and position of chlorine atoms in the aromatic ring. the most active chlorophenol converter, strain r. opacus 1cp, grew on each of the three isomeric monochlorophenols and on 2,4-dichlorophenol; the rate of growth decreased from 4-chlorophenol to 3-chlorophenol and then to 2-chlorophenol. the parameters o ... | 2000 | 10808489 |