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septic shock complicating drainage of a bartholin gland abscess. 19902216255
recolonization of the subgingival microflora after scaling and root planing in human periodontitis.the purpose of this study was to evaluate the recolonization patterns of the subgingival microflora of adult periodontitis patients after a single session of scaling and root planing. in each of eight patients, three clinically diseased sites were investigated microbiologically by darkfield microscopy and cultural analysis. after initial clinical and microbiological parameters were determined, each subject received a single session of scaling and root planing but no oral hygiene instructions. cl ...19902213468
[a case of cervico-mediastinal abscess, secondary to acute tonsillitis: investigation of the treatments].no other infectious diseases in the field of otolaryngology cause rapid and lethal course than cervical abscess. a case of cervicomediastinal abscess secondary to acute tonsillitis was presented. the patient was a 43-year-old male with liver cirrhosis and primarily had the treatment of tonsillitis. the complication of duodenal perforation caused marked general deteriotation, and cervical abscess occured. immediately after transfer to our department, he was treated by cervical drainage, laparotom ...19902213351
purification and properties of a nadh-dependent 5,10-methylenetetrahydrofolate reductase from peptostreptococcus productus.the methylenetetrahydrofolate reductase from the carbon-monoxide-utilizing homoacetogen peptostreptococcus productus (strain marburg) has been purified to apparent homogeneity. the purified enzyme catalyzed the oxidation of nadh with methylenetetrahydrofolate as the electron acceptor at a specific activity of 380 mumols.min-1 mg protein-1 (37 degrees c; ph 5.5). the apparent km for nadh was near 10 microm. the apparent molecular mass of the enzyme was determined by gel filtration to be approxima ...19902209595
susceptibility of oral bacteria to phenoxyethanol and phenoxyethanol/chlorhexidine combinations.a total of 57 bacterial strains (26 different species) which may be isolated from subgingival plaque were tested for their in vitro susceptibility to phenoxycthanol, a commonly-used antiseptic and preservative. ninety-five percent of the strains, including those associated with chronic inflammatory periodontal disease, were susceptible to concentrations of phenoxyethanol used topically (2% w/v). phenoxyethanol at a concentration of 1% (w/v) was also found that to have a rapid bactericidal effect ...19902202809
optimum therapy for acute pelvic inflammatory disease.neisseria gonorrhoeae is responsible for about one-third to one-half of cases of acute pelvic inflammatory disease (pid), although there is considerable geographical variation. chlamydia trachomatis is also an important aetiological agent, and is currently isolated 4 times more commonly from the cervix than the gonococcus. however, it is now clear that acute pid is polymicrobial in aetiology. even when n. gonorrhoeae and/or c. trachomatis are isolated from the endocervix, anaerobes such as bacte ...19902190794
parietal lobe abscess after routine periodontal recall therapy. report of a case.this paper reports on a case of a 70-year-old physician diagnosed with a parietal lobe abscess following such treatment. after stereotactic biopsy and drainage and a 6-week course of intravenous antibiotic treatment, the patient recovered with minimal neurologic deficits. although brain abscesses are not commonly encountered in practice, clinicians must be aware of the potential virulence of the anaerobic components of the periodontal pocket and the possibility of resulting systemic infection, w ...19902182824
etiology and treatment of post-cesarean-section endometritis after cephalosporin prophylaxis.to describe the microbiologic etiology of post-cesarean endometritis developing after perioperative cephalosporin prophylaxis, endometrial samples were obtained from 27 women with a triple-lumen catheter. the women were assigned in a double-blind, randomized fashion to receive either ticarcillin/clavulanate, 3.1 g, or cefoxitin, 2 g, administered every six hours, until the clinical signs of infection resolved. a total of 149 microorganisms (84 facultative and 65 obligate anaerobes) were recovere ...19902181119
cerebral ventriculitis: mr findings.cerebral ventriculitis is an uncommon site and manifestation of infection of the central nervous system. we report the magnetic resonance findings in two patients with cytologic and csf culture proof of ventriculitis. the findings included abnormal signal intensity of the ependyma and csf on t2-weighted images. in addition, ependymal, meningeal, and dural enhancement was seen in one case, on t1-weighted images, after administration of gd-diethylenetriamine pentaacetic acid.19902179305
transferable tet m in fusobacterium nucleatum.two tetracycline-resistant fusobacterium nucleatum strains were able to transfer, by conjugation, tetracycline resistance and the tet m determinant to recipient f. nucleatum, peptostreptococcus anaerobius, and enterococcus faecalis strains at a frequency of 10(-1) to 10(-8).19902178339
[improving the clinico-microbiological diagnosis and treatment of non-clostridial anaerobic peritonitis].on the basis of clinico-microbiological examinations the presence of anaerobic flora was found in 104 out of 198 patients with peritonitis. the authors propose using intra-aortal++ transfusions of oxygenated blood in complex therapy of anaerobic peritonitis.19902175490
characterization of the tet m determinants in urogenital and respiratory bacteria.tetracycline-resistant fusobacterium nucleatum, haemophilus ducreyi, mycoplasma hominis, peptostreptococcus spp., ureaplasma urealyticum, and veillonella parvula had dna sequences which showed homology throughout the length of the tet m transposon, tn916. in contrast, gardnerella vaginalis, commensal neisseria spp., and the 25.2-megadalton plasmid family lacked the complete transposon.19902159259
in vitro activity of teicoplanin, vancomycin, a16686, clindamycin, erythromycin and fusidic acid against anaerobic bacteria.the in vitro activity of teicoplanin and a16686, two new glycopeptide antibiotics was determined against 196 isolates of anaerobic bacteria. the activity of teicoplanin and a16686, in comparison with that of vancomycin, clindamycin, erythromycin and fusidic acid was 2 to 16 times higher against the gram positive anaerobes, namely, propionibacterium acnes, clostridium perfringens, clostridium difficile, clostridium species, peptococcus species and peptostreptococcus species. however, bacteroides ...19902139737
[significance of the bacterial flora in the etiology of apical periodontitis. qualitative, quantitative and topographical aspects].in the international literature it is shown the central role of root canal infection in the etiology of periapical lesions. as a matter of fact it has been proved (13) that sterile necrotic pulp tissue is completely unable to cause inflammatory reactions at the periapex. infection of endodontic origin extends to the supporting tissues of the tooth only in the case of their acute inflammation (e.g. acute apical periodontitis, acute alveolar abscess, phoenix abscess). on the other hand in chronic ...19902135040
[morpho-functional characteristics of bacterial species of major importance in endodontic infections].most of the bacteria involved in the endodontic infection can be considered as "highly specific", for they appear to be almost irrelevant in the large field of the other human disease from infection. namely the most important anaerobia responsible for endodontic infection are: bacteroides, fusobacterium, peptostreptococcus, and veillonella. the group of bacteroides is composed by several species with different metabolic and genetic characteristics. they are gram-, non-motile, non-spore-forming r ...19902133812
[a microbiologic study on anaerobic bacteremia after dental extraction].it has been known for many years that tooth extraction can cause bacterial dissemination into blood stream, that is, bacteremia. besides endocarditis--causing bacteria, alpha-hemolytic streptococci, anaerobes are involved in the post- extraction bacteremia. they are of clinical importance because they can cause abscesses in the various parenchymatous organs such as the liver, lung, and brain through anachoresis. the purpose of this study is to establish the types of species of anaerobes associat ...19902133345
a rapid diagnosis of anaerobic infection in the oro-maxillary region by gas-liquid chromatography.the relationship between volatile fatty acids (vfas) in pus and infecting bacterial species was examined in order to establish a rapid identification system for anaerobic microorganisms in purulent inflammation in the oro-maxillary region. vfas were detected by the direct injection of pus into gas-liquid chromatography (glc). bacterial examination was carried out by anaerobic culture using blood agar plates. the bacterial identification was carried out mainly according to the vpi manual. analysi ...19902131168
suppurative oral candidosis. review of the literature and report of a case.a case of a dento-alveolar abscess due to a polymicrobial infection with a yeast, candida guilliermondii and two other oral bacteria are described. a review of the literature has revealed only a single case of an odontogenic abscess due to candida species and we believe this to be the first report of a dentoalveolar infection where candida guilliermondii is associated as a co-pathogen.19902124592
intra-abdominal abscess in children: a 13-year experience.the microbiology of intra-abdominal abscess in children is similar to that of adults--a mixture of aerobes and anaerobes. in abscess specimens taken from 36 children, predominant pathogens were bacteroides fragilis group species, peptostreptococcus species, and escherichia coli.19902120268
[a clinical study of asymptomatic bacteriospermia].asymptomatic bacteriospermia was studied as an entity and its treatment was investigated in 65 subjects who clinically needed their semen tested. the obtained results are summarized as follows. 1. the rate of detection of bacteria in semen was 89.2%, and the rates of detection of aerobic and anaerobic bacteria were 55.4% and 70.8%, respectively. forty gram-positive strains were identified among the aerobic bacteria; rates of detection for staphylococcus epidermidis and enterococcus faecalis were ...19902112205
perforated jejunal diverticulum with multiple hepatic abscesses.we have described a patient with multiple hepatic abscesses caused by a perforated jejunal diverticulum with a presumed route of infection via the portal vein. patients with hepatic abscesses and no known source of infection should be evaluated for a contained mesenteric perforation of the gastrointestinal tract. finally, in patients who fail to respond promptly to percutaneous catheter drainage of a liver abscess, a continuing source of infection, such as perforation of a jejunal diverticulum, ...19902105531
the effect of metronidazole on the anaerobic microorganisms of the root canal--a clinical study.the antibacterial effect of 0.25% metronidazole solution as root canal irrigant on anaerobic bacteria was studied in ten central incisors. each tooth was treated at four appointments, and the presence of anaerobes in the root canal was studied on each occasion. no antibacterial intracanal dressings were used between the appointments. no anaerobes could be recovered from any tooth on the fifth appointment. in the control group, where normal saline was used as an irrigant, anaerobes could be recov ...19902100667
bactericidal effects of human neutrophils and sera on selected endodontic pathogenic bacteria in an anaerobic environment.phagocytosis by normal human neutrophils and the bactericidal activity of normal human pooled serum were measured under anaerobic conditions on six endodontic pathogenic bacterial species from the genera actinomyces, bacteroides, fusobacterium, peptostreptococcus and streptococcus. the results revealed that actinomyces viscosus was not killed either by human neutrophils or by 20 per cent serum; peptostreptococcus anaerobius was not killed by either 20 per cent or 95 per cent serum; and bacteroid ...19902098335
an in vitro method to study the adherence of bacteria to saliva-treated tooth enamel sections.an in vitro bacterial adherence assay which employed human tooth enamel sections precoated with saliva and an epifluorescent staining technique with acridine orange was developed. the assay was used to study the adherence properties of fresh and type strains of the following oral bacterial species: bacteroides gingivalis, bacteroides intermedius, capnocytophaga species, haemophilus aphrophilus, actinobacillus actinomycetemcomitans, peptostreptococcus species, veillonella species, actinomyces isr ...19902087341
[susceptibilities of clinical bacterial isolates to antimicrobial agents. a study mainly focused on imipenem. research group for testing imipenem susceptibility on clinical isolates].we investigated susceptibilities of clinical bacterial isolates to imipenem (ipm) and other antimicrobial agents at 459 hospital laboratories throughout japan from september to december of 1988. in this study, identification and susceptibility testing were performed at each hospital laboratory and the tests were carried out according to the 1-dilution or 3-dilution disc technique in which susceptibilities are classified into 4 grades: , ++, + and -. ipm had significantly high activity against st ...19902086814
localization and identification of root canal bacteria in clinically asymptomatic periapical pathosis.twenty-one teeth with clinically asymptomatic periapical pathosis (class 3) were extracted and the isolation, identification, and localization of bacteria in the root apex were examined. mixtures involving several bacteria were isolated from more than 60% of the cases. scanning electron microscopy revealed bacterial masses to be associated with the apical part of the root canal, but not with the area of apical foramen or on the surface of root apex. our results indicate that the bacteria in clas ...19902084210
the formation of hydrogen sulfide and methyl mercaptan by oral bacteria.the capacity to form volatile sulfur compounds was tested in bacteria isolated from subgingival microbiotas and in a representative number of reference strains. a majority of the 75 tested oral bacterial species and 7 unnamed bacterial taxa formed significant amounts of hydrogen sulfide from l-cysteine. the most active bacteria were found in the genera peptostreptococcus, eubacterium, selenomonas, centipeda, bacteroides and fusobacterium. methyl mercaptan from l-methionine was formed by some mem ...19902082242
polysaccharides from peptostreptococcus anaerobius and structure of the species-specific antigen.the cell-envelope antigens of peptostreptococcus anaerobius were extracted from intact cells by autoclave or alkaline treatment. the purified species-specific antigen (g) was identified among several polysaccharides obtained from the extracts by successive treatments with ribonuclease and pronase followed by ion-exchange and gel-filtration chromatography. g was investigated by 13c- and 31p-n.m.r. spectroscopy, titrimetry, elemental analysis, and gas-liquid chromatography. oxidation of g with nai ...19902076510
chronic prosthetic vascular graft infection visualized with technetium-99m-hexamethylpropyleneamine oxime-labeled leukocytes.technetium-99m-hmpao labeled leukocytes demonstrated chronic femoro-femoral prosthetic vascular graft infection several times during an 18-mo period in a 77-yr-old man. the intensity and distribution of the uptake in the graft were fluctuating in different imaging occasions possibly indicating the strength and location of the infection. gallium-67-citrate imaging showed negative results twice. the reason for negative 67ga results remained obscure. the infected graft was removed and the patient d ...19912066799
purification and properties of an iron-sulfur-containing and pyridoxal-phosphate-independent l-serine dehydratase from peptostreptococcus asaccharolyticus.l-serine dehydratase with a specific activity of 15 nkat/mg protein was present in the anaerobic eubacterium peptostreptococcus asaccharolyticus grown either on l-glutamate or l-serine. the enzyme was highly specific for l-serine with the lowest km = 0.8 mm ever reported for an l-serine dehydratase. l-threonine (km = 22 mm) was the only other substrate. v/km for l-serine was 500 times higher than that for l-threonine. l-cysteine was the best inhibitor (ki = 0.3 mm, competitive towards l-serine). ...19912065681
pyoderma caused by peptostreptococcus tetradius in a pup.pyoderma caused by peptostreptococcus tetradius in a pup was successfully treated with cefadroxil. although this bacterium is rarely found in veterinary medicine, it is a known pathogen in human beings and should be considered as a potential causative agent in pyodermas in which anaerobes are suspected.19912061185
the differentiation of asaccharolytic anaerobic gram-positive cocci by protein electrophoresis.conventional biochemical and antibiotic sensitivity tests were used to allocate 87 clinical isolates of anaerobic gram-positive cocci to currently recognised species, in comparison with type and other authentic reference strains. whole-cell protein electrophoresis was then performed with extracts of each strain. allowing for difficulties of standardisation, it was possible to allocate most of the organisms to species-related groups on the basis of protein patterns. organisms identified conventio ...19912056518
vaginal bacteroides species are associated with an increased rate of preterm delivery among women in preterm labor.the relationship of high concentrations of vaginal microorganisms with an increased rate of preterm delivery (less than or equal to 34 weeks) among women in preterm labor was evaluated. quantitative vaginal cultures were obtained from 211 women in preterm labor between gestational ages of 22 and 34 completed weeks. preterm delivery occurred in 139 (66%). the rate of preterm delivery was determined by cox proportional hazards models. women with greater than 10(7) cfu/ml of vaginal fluid of facult ...19912056221
[antibacterial activity of ciprofloxacin against fresh clinical isolates from superficial suppurative foci].the minimum inhibitory concentrations (mics) of 5 drugs (ciprofloxacin (cpfx), and 4 drugs used as standard) were determined to investigate antibacterial potencies of cpfx against bacterial strains isolated in 1989 from superficial suppurative foci. the clinical isolates tested included 375 strains from 11 aerobic bacterial species, and 50 strains from 2 anerobic bacterial genera (group) for a total of 425 isolates. interpreting mic level distributions of these drugs as the expression of antibac ...19912041153
adhesion of 1-carbon utilizing bacteria to polymeric surfaces. 19912037198
oral associated bacterial infection in horses: studies on the normal anaerobic flora from the pharyngeal tonsillar surface and its association with lower respiratory tract and paraoral infections.two hundred and seventy bacterial isolates were obtained from the pharyngeal tonsillar surface of 12 normal horses and 98 obligatory anaerobic bacteria were characterised. of these, 57 isolates belonging to 7 genera (peptostreptococcus (1); eubacterium (9); clostridium (6); veillonella (6); megasphera (1); bacteroides (28); fusobacterium (6)) were identified, and 16 of these were identified to species level (p. anaerobius (1); e. fossor (9); c. villosum (1); b. fragilis (1); b. tectum (2); b. he ...19912031304
the laboratory identification of gram-positive anaerobic cocci.a collection of 256 clinical strains and 40 reference strains of gram-positive anaerobic cocci (gpac) was studied, to characterise the recognised species more fully and to define groups of strains which might correspond to previously undescribed species. the methods used were: gas-liquid chromatography (glc) for the detection of volatile fatty acids (vfas); determination of the pre-formed enzyme profile with a commercially available kit, atb 32a; microscopic appearance; colonial morphology; and ...19912030504
toothbrushing and transient bacteremia in patients undergoing orthodontic treatment.this study was made to determine whether and to what extent bacteremia occurred after toothbrushing in patients undergoing orthodontic treatment with fixed appliances. twenty patients were selected, all with negative history of heart or hematologic disorders. these patients had not taken antibiotics or had a history of a cold in the previous 30 days. blood samples of 20 ml were drawn before and 5 minutes after brushing. the immune status of the patients was tested by measurement of isohemaggluti ...19912028936
microbiological studies of decubitus ulcers in children.specimens from 58 children with decubitus ulcers were cultured for aerobic and anaerobic bacteria. aerobic bacteria only were present in 29 (50%) ulcers, anaerobic bacteria only were recovered in 5 (9%), and mixed aerobic and anaerobic flora were present in 24 (41%). a total of 132 isolates (79 aerobes, 53 anaerobes) were recovered, an average of 2.3 isolates per specimen (1.4 aerobes, 0.9 anaerobes). the smallest number of isolates was recovered in ulcers of the skull (1.7 per site), and the hi ...19912023086
metabolism of 1,4-dinitro-2-methylpyrrole, a mutagen formed by a sorbic acid-nitrite reaction, by intestinal bacteria.1,4-dinitro-2-methylpyrrole (dnmp), a mutagenic product formed by the interaction of two common food additives, sorbic acid and sodium nitrite, was transformed to 1-nitro-2-methyl-4-aminopyrrole (nmap) by human fecal mixtures and various intestinal bacterial strains. under anaerobic conditions the cell suspensions of actinomyces, bacteroides, clostridium, eubacterium, fusobacterium, and peptostreptococcus spp. demonstrated the nitroreduction activity. under aerobic conditions, only actinomyces a ...19912022195
microbiological study of hiv-related periodontitis.the subgingival microbiota in 14 persons with hiv-periodontitis was examined. subgingival plaque samples were collected with paper points, transported in vmga iii, and plated on anaerobic enriched brucella blood agar and various selective media. hiv-periodontitis sites revealed actinobacillus actinomycetemcomitans, wolinella recta, peptostreptococcus micros, and bacteroides intermedius, each averaging 7% to 16% of the cultivable subgingival flora in positive patients. high levels of spirochetes ...19912002434
aerobic and anaerobic microbiology of peritonsillar abscess.thirty-four aspirates of pus from peritonsillar abscesses that were studied for aerobic and anaerobic bacteria showed bacterial growth. a total 107 bacterial isolates (58 anaerobic and 49 aerobic and facultative) were recovered, accounting for 3.1 isolates per specimen (1.7 anaerobic and 1.4 aerobic and facultatives). anaerobic bacteria only were present in 6 (18%) patients, aerobic and facultatives in 2 (6%), and mixed aerobic and anaerobic flora in 26 (76%). single bacterial isolates were reco ...19912000017
aerobic and anaerobic microbiology of acute suppurative parotitis.aspirates of pus from acute suppurative parotitis were studied for aerobic and anaerobic bacteria. bacterial growth was present in 23 specimens. a total of 36 bacterial isolates (20 anaerobic and 16 aerobic and facultative) were recovered, accounting for 1.6 isolates per specimen (0.9 anaerobic and 0.7 aerobic and facultative). anaerobic bacteria only were present in 10 (43%) patients, aerobic and facultatives in 10 (43%), and mixed aerobic and anaerobic flora in 3 (13%). single bacterial isolat ...19911992268
[anaerobic bacteria and their role in recurrent tonsillitis].the presence of anaerobic bacteria in 100 palatal tonsils obtained during the tonsillectomy, was evaluated in quality and in quantity. 17 species of anaerobic bacteria were found. the most frequent were: bacteroides melaninogenicus, peptostreptococcus anaerobius, peptostreptococcus intermedius, propionibacterium, veillonella parvula and peptococcus asaccharolyticus. in most evaluations their density was 10(5)-10(6) in gram of tonsil tissue, sometimes 10(7). its role in recurrent tonsils infectio ...19901981606
summer mastitis in heifers: a bacteriological examination of secretions from clinical cases of summer mastitis in denmark.a total of 166 samples of secretions collected from danish heifers suffering from clinically diagnosed summer mastitis were examined bacteriologically. one hundred and sixty three samples yielded positive findings whereas no growth was obtained from 3 specimens. the majority of samples revealed a mixed flora in which the predominant components were actinomyces pyogenes, peptostreptococcus indolicus, a microaerophilic coccus (stuart-schwan coccus), fusobacterium necrophorum, bacteriodes melaninog ...19901973009
comparative efficacy of cefoperazone, cefoperazone plus sulbactam, ciprofloxacin, clindamycin, metronidazole, and penicillin g against anaerobic bacteria in an animal model.treatment efficacy of various antimicrobial regimens against anaerobes was studied in semipermeable chambers simulating a closed-space, locally neutropenic infection site in rabbits. bacteroides fragilis, bacteroides melaninogenicus, clostridium perfringens, and peptostreptococcus anaerobius were inoculated (at a mean of 5.3 log10 cfu/ml in prereduced pooled rabbit serum) into the chambers (one isolate per chamber) in triplicate. antimicrobial therapy consisted of cefoperazone, cefoperazone plus ...19901967626
[antibacterial activities of ofloxacin against recent isolates from patients with community-acquired infections].in order to survey antibacterial activities of ofloxacin (oflx) against 1,440 bacterial strains isolated from patients with community-acquired infections in 1987 and 1990, minimum inhibitory concentrations (mics) of the drug as well as those of other new quinolones and oral cephems were determined. the following conclusions were reached. 1. comparison of the mic distribution for strains isolated in 1987 with those in 1990 suggested a tendency toward an increase in the frequency of oflx-resistant ...19911960856
the partial amino acid sequence of the nad(+)-dependent glutamate dehydrogenase of clostridium symbiosum: implications for the evolution and structural basis of coenzyme specificity.the amino acid sequence is reported for cnbr and tryptic peptide fragments of the nad(+)-dependent glutamate dehydrogenase of clostridium symbiosum. together with the n-terminal sequence, these make up about 75% of the total sequence. the sequence shows extensive similarity with that of the nadp(+)-dependent glutamate dehydrogenase of escherichia coli (52% identical residues out of the 332 compared) allowing confident placing of the peptide fragments within the overall sequence. this demonstrate ...19911954226
effects of propicillin on mixed continuous cultures of periodontal bacteria.experiments were designed to test the antibiotic (1-phenoxypropyl)penicillin (propicillin) against a complex microflora of periodontal bacteria. this was accomplished by using a continuously growing mixed culture that was obtained by enrichment of periodontal plaque in human serum. peptostreptococcus species, prevotella intermedia, lactobacillus, catenaforme, and streptococcus species were dominant members of the enrichment culture. none of the strains isolated from the enrichment culture exhibi ...19911952836
microbiologic causes and neonatal outcomes associated with chorioamnion infection.chorioamnion infection is associated with histologic chorioamnionitis and prematurity, but the specific chorioamnion microorganisms associated with histologic chorioamnionitis, prematurity, and poor neonatal outcome have not been identified. bacteria were recovered from the chorioamnion cultures of 32% of 112 placentas delivered at less than or equal to 34 weeks' gestation and from 19% of 156 placentas delivered at greater than 34 weeks' gestation (odds ratio 2.1; 95% confidence interval 1.1 to ...19911951562
specificity of antibodies present in human periapical lesions.various classes of immunoglobulins have been found in human periapical lesions. the specificity of secreted antibodies against antigens egressing from the root canal system has yet to be thoroughly investigated. the purpose of this study was to test the specificity of antibodies present in human periapical lesions. human periapical biopsies were removed and cultured as organ culture explants. antibodies present in the lesions were extracted in the cell culture fluids. a modified enzyme-linked im ...19911940740
reactivity of igg from explant cultures of periapical lesions with implicated microorganisms.the presence of igg in periapical inflammatory lesions suggests that immune responses participate in the disease process. the purpose of this investigation was to study the reactivity of igg from the supernatant fluids of explant cultures of periapical lesions with microorganisms implicated in infections of endodontic origin. ninety periapical lesions that had been contiguous with the apex of a root were removed and maintained in explant cultures. a dot-enzyme-linked immunosorbent assay (dot-eli ...19911940739
cos degradation by selected co-utilizing bacteria. scientific note. 19911929384
tetracycline resistance in peptostreptococcus species.of 15 peptostreptococcus sp. strains isolated between 1975 and 1984, 13 hybridized with the tet k, tet m, or tet o determinant. a donor peptostreptococcus anaerobius strain carrying the three determinants could transfer tet m to p. anaerobius and fusobacterium nucleatum recipients but not to an enterococcus faecalis recipient, while neither tet k nor tet o was transferred.19911929341
[clinical evaluation of imipenem/cilastatin sodium in the internal medicine].fifty-two patients with moderate or severe infections associated with internal medicine were treated with imipenem/cilastatin sodium (ipm/cs) and the efficacy and the safety of this drug were evaluated. there were 20 patients with pneumonia, 10 with acute exacerbation of chronic respiratory tract infections, 9 with sepsis, 2 with pyothorax, 3 with intraabdominal infection, 2 with urinary tract infection, 1 with pulmonary abscess, 1 with infective endocarditis, 4 with fever of unknown origin. for ...19911920813
selection, expression, and nucleotide sequencing of the glutamate dehydrogenase gene of peptostreptococcus asaccharolyticus.the gene for the catabolic nad-linked glutamate dehydrogenase of peptostreptococcus asaccharolyticus was cloned by selection of escherichia coli for complementation of a biosynthetic defect. cloned fragments containing the gene and the p. asaccharolyticus transcription and translation signals are very highly expressed in e. coli. the nucleotide sequence of the cloned gene was determined. it codes for a polypeptide of 421 amino acids, the sequence of which is similar to those of the nadp-acceptin ...19911917850
protein-kinase-c-independent activation of arachidonate release and prostaglandin e2 formation in macrophages interacting with certain bacteria.certain bacterial species, of which we selected fusobacterium nucleatum, gardnerella vaginalis, peptostreptococcus anaerobius and propionibacterium acnes, were found to induce release of arachidonic acid in a dose- and time-dependent manner in mouse macrophages. the release of arachidonic acid showed a characteristic lag period of approximately 10 min and was accompanied by selective transformation into prostaglandin e2. bacteria killed by various methods caused a similar response, indicating th ...19911915341
bacteriologic monitoring of periodontal diseases: cultural, enzymatic, immunologic, and nucleic acid studies.evidence supporting the bacteriologic etiology of periodontal diseases has led investigators to monitor the microbiologic species associated with each disease. the type of study performed has evolved at different levels over the years and is predominantly influenced by available technologies. as the technology has advanced, so too have attempts at monitoring specific microorganisms. the ultimate goal in most research efforts on clinical bacteriologic monitoring of the periodontal diseases has be ...19911912631
suitability of the broth-disk elution test for evaluating susceptibility of obligate anaerobes to trimethoprim-sulfonamides.species of anaerobic bacteria isolated from clinical veterinary specimens were tested for susceptibility to trimethoprim-sulfonamides by the broth-disk elution technique. three different media were used for each organism: prereduced anaerobically sterilized (pras) brain-heart infusion broth (bhi), thioglycollate broth, and a semidefined pras medium. susceptibility results from these media were compared with those determined by interpreting the minimal inhibitory concentration obtained using an a ...19911911991
[hemolytic jaundice in anaerobic bacteremia. a report of a case resolved surgically].the authors report a case of haemolytic jaundice caused by anaerobic bacteria arising from an abdominal abscess. this is undoubtedly a rare pathology but, when occurring, the resolution may be achieved by surgical therapy.19911911074
microbial aspects of failing osseointegrated dental implants in humans.the subgingival microflora of 18 failing implants were examined for pathogenic periodontal microorganisms. peptostreptococcus micros was recovered from 6 failing implants, wolinella recta from 6, fusobacterium species from 5, candida albicans from 5, and bacteroides intermedius from 4. enteric rods or pseudomonads constituted a significant part of the microflora in 5 failing implants. actinobacillus actinomycetemcomitans, nonpigmented bacteroides species, capnocytophage species, and staphylococc ...19911906537
purification and characterization of nadp(+)-dependent 5,10-methylenetetrahydrofolate dehydrogenase from peptostreptococcus productus marburg.the 5,10-methylenetetrahydrofolate dehydrogenase of heterotrophically grown peptostreptococcus productus marburg was purified to apparent homogeneity. the purified enzyme catalyzed the reversible oxidation of methylenetetrahydrofolate with nadp+ as the electron acceptor at a specific activity of 627 u/mg of protein. the km values for methylenetetrahydrofolate and for nadp+ were 27 and 113 microm, respectively. the enzyme, which lacked 5,10-methenyltetrahydrofolate cyclohydrolase activity, was in ...19911899860
[2 cases of rhinogenic intracranial complications with normal rhinoscopy].two cases of non-postoperative rhinogenic intracranial complications are reported: patient 1 was a 25-year-old female with meningitis and right orbital phlegmon, and patient 2 was a 21-year-old male with a brain abscess. main symptoms in these patients were meningeal signs and high fever, and intranasal findings were normal in both patients. the diagnosis was not made until computed tomography revealed paranasal abnormalities. soon after surgical intervention and treatment with large doses of an ...19911896694
chronic conjunctivitis caused by oral anaerobes and effectively treated with systemic metronidazole plus amoxicillin.in this study, we report on a case of refractory, unilateral anaerobic conjunctivitis. the predominant anaerobic flora consisted of prevotella intermedia (formerly bacteroides intermedius) and peptostreptococcus micros. by using the technique of restriction endonuclease fingerprinting of genomic dna, it was shown that the p. intermedia likely originated from the oral cavity. topically applied antibiotics had failed to suppress the infection in the past. successful treatment was achieved after sy ...19911890173
the importance of positive bacterial cultures of specimens obtained during clean orthopaedic operations.microbiological cultures of specimens of tissue and of fluids from the wound in forty patients who had had consecutive clean, elective orthopaedic operations (excluding total joint replacements) and had not received antibiotics preoperatively were analyzed. of the forty patients, twenty-three (58 per cent) had a positive culture on at least one of the media that were used and seventeen (43 per cent) had negative cultures. of the forty specimens that were obtained from swabbing of the wound, eigh ...19911890121
[diagnosis of contact mediastinitis in necrotic phlegmons of the neck]. 19911882299
[randomized study of amoxicillin-clavulanic acid versus amoxicillin-metronidazole combinations in pyogenic infections of dental origin].thirty patients, without any valve defect, cardiac malformation or previous antibiotic administration and presenting dental infections, have been treated either with amoxycillin-clavulanic acid or amoxycillin and metronidazole. aerobic and anaerobic bacteria have been isolated. these infections were due to mixed flora. nearly one half of anaerobes were peptostreptococcus, s. milleri and s. sanguis being predominant among aerobes. the species identified were those found in the mouth in the absenc ...19911881695
[clinical study on cefteram pivoxil in asymptomatic bacteriospermia].in order to understand the clinical efficacy of cefteram pivoxil (cftm) in the treatment of asymptomatic bacteriospermia, the following studies were performed. 1. concentrations of cftm in the semen after oral administration of 200 mg to normal healthy adults (n = 5) reached a maximum level of 0.66 +/- 0.04 microgram/ml in 5 hours after the administration, then decreased rapidly, and averaged 0.15 +/- 0.03 micrograms at 7 hours after administration. 2. activities of cftm, cefaclor (ccl) and lome ...19911880933
nosocomial infections of ocular conjunctiva in newborns delivered by cesarian section.colonization of the ocular conjunctiva in newborns delivered by cesarian section occurs usually within the first day of life. we have studied the flora of the ocular conjunctiva at birth, from 19 newborns delivered by cesarian section, coming from two different maternity hospitals. ocular conjunctiva cultures yielded the main predominant flora in both maternity hospitals considered. the most common genus of this flora are: staphylococcus, corynebacterium and propionibacterium acnes. peptostrepto ...19911870845
antibiosis between bacteria isolated from the vagina of women with and without signs of bacterial vaginosis.lactobacilli from women with and without bacterial vaginosis (bv) were tested for h2o2 production. thirty-seven (79%) of the 47 strains of lactobacilli isolated from the women without bv produced h2o2, while only nine (23%) of the 39 strains of lactobacilli obtained from women with bv did so. five of 20 h2o2-producing and two of 26 non-producing strains of lactobacillus exhibited antibiosis against four of 12 strains of peptostreptococci and two of 10 strains of mobiluncus spp. none of a further ...19911859646
bacteriological investigations of clinical mastitis in heifers in sweden.bacterial analyses were carried out of 2069 udder secretions, isolated from 1481 heifers with mastitis in eight veterinary districts in sweden. streptococci, e.g. streptococcus dysgalactiae and str. uberis, dominated the bacterial flora, being isolated from 34.4 and 19.5% respectively of heifers with clinical mastitis occurring from puberty up to 14 d post partum. bacterial species generally regarded as important pathogens in the summer mastitis complex, e.g. actinomyces pyogenes, stuart-schwan ...19911856352
anaerobic pathogenesis: collagenase production by peptostreptococcus magnus and its relationship to site of infection.fifty isolates of peptostreptococcus magnus from intraabdominal sepsis, nonpuerperal breast abscess, and diabetic foot infections were examined for collagenase activity using bovine type i collagen. collagenase production was detected in a higher percentage of strains from nonpuerperal breast and diabetic foot specimens (p less than .001). this enzyme may be responsible for p. magnus playing a more central role in the pathogenesis of nonpuerperal breast abscess and diabetic foot disease than in ...19911850443
microbial differences in 2 clinically distinct types of failures of osseointegrated implants.the microflora associated with osseointegrated implants suspected of failing from infection or trauma were evaluated. of 75 patients examined, 11 demonstrated failing fixtures. 22 of 48 fixtures failed in 6 fully edentulous patients and 10 of 34 failed in 5 partially edentulous patients. the etiology of failure was attributed to infection if there was bleeding, suppuration, pain, high plaque and gingival indices and granulomatous tissue upon surgical removal. traumatic etiology was suspected in ...19911843467
clinical and microbiological findings on newly inserted hydroxyapatite-coated and pure titanium human dental implants.the clinical and microbiologic features of 30 hydroxyapatite-coated root-form endosseous dental implants (tri-stage) were compared to 10 similar pure titanium implants without hydroxyapatite coatings. in 7 of 9 partially edentulous patients studied, pure titanium fixtures were placed adjacent to hydroxyapatite-coated implants. implants in the maxilla were submerged beneath mucosal tissues after implant placement for a minimum of 6 months, and in the mandible for at least 4 months. all patients w ...19911843465
microbial risk indicators for periodontal attachment loss. 19911831856
native valve infective endocarditis in adults--analysis of 32 consecutive cases over a ten-year period from 1980 to 1989.thirty-two patients with native valve infective endocarditis who presented over a 10-year period at our hospital were analyzed retrospectively. the presenting symptom was a persistent fever in 22 patients. in 30 patients, the new york heart association functional class was less than ii before the development of endocarditis. blood cultures were positive on all occasions in 24 out of 29 culture-positive patients. all of the viridans streptococci, accounting for 79% of the isolated pathogens, were ...19911829492
predominant cultivable flora in pericoronitis.the predominant cultivable flora in pericoronitis was investigated by culturing pus from affected sites in 20 patients. twenty colonies were picked at random from non-selective plates and identified using conventional biochemical and physiological tests, analysis of metabolic end-products by gas chromatography and protein profile analysis. the most frequently isolated organisms were prevotella (bacteroides) intermedia, peptostreptococcus micros, veillonella species, fusobacterium nucleatum and s ...19911820571
formation of nitrogen-containing metabolites from geniposide and gardenoside by human intestinal bacteria.during the course of our studies on the metabolism of iridoid glycosides by human intestinal bacteria, we found that geniposide (1) and gardenoside (4) were transformed to new nitrogen-containing compounds, genipinine (3) and gardenine (6), respectively, along with the known aglycones. although the amounts of new metabolites were somewhat lower than those of the aglycones, they were quantitatively analyzed by means of liquid chromatography/mass spectrometry (lc/ms). of 25 strains of human intest ...19911818345
bacteria in the apical 5 mm of infected root canals.ten freshly extracted teeth which had carious pulpal exposures and periapical lesions contiguous with the root apex were placed inside an anaerobic chamber and the apical 5 mm of the root canals cultured. in addition to anaerobic incubation, duplicate cultures were incubated aerobically. fifty strains of bacteria from the 10 root canals were isolated and identified. the most prominent bacteria cultured from the 10 root canals were actinomyces, lactobacillus, black-pigmented bacteroides, peptostr ...19911809801
[empyema due to an anaerobic streptococcus in childhood].anaerobic infections are quite rare in pediatric age, being that, they affect only neonates and immunodepressed patients. we think to be somewhat interesting to describe the case of our patient, a 9 year old boy, unaffected by any predisposing factor, came under our observation because of a severe respiratory distress. he showed evident clinical and radiological signs of pleural effusion in the right lung, together with a gas coil in the upper field and a left mediastinal shifting. a thoracentes ...19911806927
[effects of antibiotics and anti-bacterial components of preparations for local treatment of suppurative wounds on pathogens of odonto- genic infections].the results of identification and sensitivity assay of 156 strains of pathogens causing odontogenic infections are presented. in the sensitivity assay antibacterial drugs were used. 42.3 percent of the strains were obligate anaerobes belonging to bacteroides, fusobacterium, peptococcus, peptostreptococcus, veillonella and actinomyces. significant differences in the microbial sensitivity to the drugs used for general and local therapy were detected. there was observed high sensitivity of the obli ...19911805697
normal vaginal aerobic and anaerobic bacterial flora of the rhesus macaque (macaca mulatta).the most common bacterial species isolated from the vaginas of 37 healthy rhesus macaques were streptococcus viridans, coagulase negative staphylococcus, mobiluncus curtisii ss. curtisii, corynebacterium renale-like organisms, peptostreptococcus anaerobius, gardnerella-like organisms, and other corynebacterium species. the vaginal flora of the rhesus macaque differs from that previously reported for five other primate species. a two-year retrospective review of clinical cases of vaginitis and me ...19911803013
comparative in vitro activity of cefpodoxime against anaerobes other than bacteroides fragilis.to assess the in vitro activity of cefpodoxime against anaerobic respiratory tract and oropharyngeal pathogens 77 strains belonging to 18 gram-negative and 7 gram-positive species were studied by means of agar dilution tests. for comparison cefuroxime, amoxicillin, amoxicillin + clavulanic acid and clindamycin were also tested. cefpodoxime was found to be active at concentrations of less than or equal to 0.125 mg/l against prevotella oralis, prevotella buccalis, prevotella bivia, porphyromonas a ...19911800380
[bacteriological examination of infections in the field of oral surgery].etiology of bacterial infections in the field of oral surgery was studied. a total of 270 samples collected from patients with encapsulated abscess in their oral cavities was examined and bacteria were isolated from the 244 samples (90.4%). the following results were found; 1) organisms more than one from one sample were frequently isolated from cases with parodontitis, pericoronitis and gnathitis. isolation of anaerobic bacteria was common (54.2%). 2) streptococcus milleri and streptococcus san ...19911791332
[analysis of 98 cases of thoracic empyema].ninety-eight cases of empyema thoracis admitted to juntendo university hospital between 1979 and 1990 were reviewed. males accounted for 78 cases and females 20 cases. thirteen pediatric patients ranged in age from 17 days to 4 years, while the 85 adult cases ranged from 16 to 89 years (mean: 58.4 years). the mortality rate increased with age. fifty-three cases of community-acquired empyema thoracis consisted of 24 with no underlying disease (including 13 pediatric cases), and 29 with diabetes m ...19911783810
[a classic case of ludwig's angina].the aa. have the chance to review the etiology, the clinic and the management of this risky process, through a characteristic ludwig's angina of their own practice. a middle age man carrying an odontogenic inflammation developed a spreading infection into the deep neck tissues. the problem was successfully resolved with antibiotherapy and surgical drainage.19911781512
[chronic conjunctivitis caused by oral anaerobic germs].a patient with chronic, unilateral conjunctivitis is presented. the predominant bacterial flora consisted mainly of prevotella intermedia (formerly bacteroides intermedius) and peptostreptococcus micros. restriction endonuclease fingerprinting of genomic dna demonstrated that p. intermedia most probably originated from the oral cavity. in the oral cavity this species was recovered from the dorsum of the tongue, the tonsillar area and the periodontal pockets. topical treatment with various antibi ...19911758517
the microflora of periodontal sites showing active destructive progression.20 adult periodontitis (ap) subjects were examined every 2 to 4 months and microbiological samples were collected and cultured when 2 mm or more loss of attachment (active sites) was detected by 2 examiners. similar sites in which no progressive destruction was observed (control sites) also were sampled in the same subjects. by lambda-analysis, there was no statistically significant difference in floras of active (42 sites from 12 subjects) and control (36 sites from 12 subjects) sites or betwee ...19911752997
bacterial vaginosis.bacterial vaginosis (bv) is the most common of the vaginitides affecting women of reproductive age. it appears to be due to an alteration in the vaginal ecology by which lactobacillus spp., the predominant organisms in the healthy vagina, are replaced by a mixed flora including prevotella bivia, prevotella disiens, porphyromonas spp., mobiluncus spp., and peptostreptococcus spp. all of these organisms except mobiluncus spp. are also members of the endogenous vaginal flora. while evidence from tr ...19911747864
severe anaerobic necrotizing pneumonia complicated by pyopneumothorax and anaerobic monoarthritis due to peptostreptococcus magnus.a rare case of a severe anaerobic necrotizing pneumonia complicated by pyopneumothorax and monoarthritis due to anaerobic peptostreptococcus magnus is described in a 20-year-old soldier. the patient's immunological status was normal. there were no predisposing underlying factors, and he was treated successfully without any residual damage. to our knowledge, a similar case has never been reported in the literature.19911745861
the relationship of hydrogen peroxide-producing lactobacilli to bacterial vaginosis and genital microflora in pregnant women.lactobacilli provide an important microbial defense against genital colonization by pathogens. the role of hydrogen peroxide (h2o2) in the control of genital microflora was explored in a cross-sectional study of 275 women in the second trimester of pregnancy. vaginal cultures were obtained for detection of h2o2-positive and h2o2-negative lactobacilli and other members of the genital microflora. compared with women with h2o2-negative lactobacilli, women colonized by h2o2-positive lactobacilli wer ...19921738516
multicentre study on antibiotic susceptibilities of anaerobic bacteria to cefoperazone-sulbactam and other antimicrobial agents.the antibiotic susceptibilities of 241 anaerobic bacteria recovered from six geographic sites in north america were tested by agar dilution to cefoperazone-sulbactam and other drugs. of the 189 bacteroides fragilis group isolates, only one was resistant to cefoperazone-sulbactam (0.5%) or ampicillin-sulbactam (0.5%), and none was resistant to ticarcillin-clavulanate or chloramphenicol. no resistance to cefoperazone-sulbactam was observed among the other bacteroides spp., clostridium spp., or pep ...19921737725
protein l from peptostreptococcus magnus binds to the kappa light chain variable domain.protein l is an immunoglobulin light chain-binding protein expressed by some strains of the anaerobic bacterial species peptostreptococcus magnus. the major variable region subgroups of human kappa and lambda light chains were tested for protein l binding; v kappa i, v kappa iii, and v kappa iv bound protein l, whereas no binding occurred with proteins of the v kappa ii subgroup or with any lambda light chain subgroups. studies of the protein l binding capacity of naturally occurring vl fragment ...19921733930
changes in the microbial flora of airline headset devices after their use.the bacterial flora of 20 headset devices were evaluated before and after they were worn for 1 hour. bacteria were recovered from all headsets, and their number increased from a mean (+/- standard deviation) of 60 +/- 5 organisms per device to 650 +/- 51 organisms per device (p less than .001). the predominant organisms recovered were staphylococcus spp, streptococcus spp, propionibacterium spp, and peptostreptococcus spp. this study demonstrates the presence of potential pathogens in headset de ...19921731163
diagnosis and management of anaerobic infections of the head and neck.anaerobic bacteria are important pathogens in head and neck infections such as chronic otitis media, chronic sinusitis, chronic mastoiditis, head and neck abscesses, cervical adenitis, parotitis, and postoperative infection. bacteroides sp (bacteroides melaninogenicus group, bacteroides oralis, and bacteroides fragilis group), peptostreptococcus sp, and fusobacterium sp predominate. the observed recent increase in the number of beta-lactamase-producing strains of bacteroides sp isolated in head ...19921728904
molecular fingerprinting of isolates of the genus peptostreptococcus using rrna genes from escherichia coli and p. anaerobius.restriction fragment length polymorphisms (rflps) of rrna genes were evaluated as a tool for intra- and interspecies differentiation of peptostreptococcus isolates. rflps from a collection of 20 clinical isolates and five atcc strains representing five peptostreptococcus spp. (p. anaerobius, p. asaccharolyticus, p. magnus, p. micros and p. prevotii) were obtained by hybridization of southern blots of hindiii- or ecori-digested genomic dna with three probes: probe a, a 0.98 kb hindiii fragment wi ...19911717639
protein l. a bacterial ig-binding protein that activates human basophils and mast cells.peptostreptococcus magnus strain 312 (10(6) to 10(8)/ml), which synthesizes a protein capable of binding to kappa l chains of human ig (protein l), stimulated the release of histamine from human basophils in vitro. p. magnus strain 644, which does not synthesize protein l, did not induce histamine secretion. soluble protein l (3 x 10(-2) to 3 micrograms/ml) induced histamine release from human basophils. the characteristics of the release reaction were similar to those of rabbit igg anti-fc frag ...19901698870
differential effects of granulocyte-macrophage colony-stimulating factor and granulocyte colony-stimulating factor in children with severe congenital neutropenia.severe congenital neutropenia (scn) is a disorder of myelopoiesis characterized by severe neutropenia secondary to a maturational arrest at the level of promyelocytes. we treated five patients with scn with recombinant human granulocyte-macrophage colony-stimulating factor (rhgm-csf) for 42 days and subsequently, between 1 and 3 months later, with rhg-csf for 142 days. the objective was to evaluate the safety and ability of these factors to elicit a neutrophil response. rhgm-csf was administered ...19901689595
[acute subdural empyema due to peptostreptococcus].a very rare case of acute subdural empyema due to peptostreptococcus was reported. a 11-year-old-girl was admitted to our hospital with high grade fever, unconsciousness and rt hemiparesis. ct scans showed the mass effect caused by the subdural empyema over the left frontotemporal region. subdural empyema was evacuated by the craniotomy. peptostreptococcus was found in the pus obtained during the operation. however, ct scans 10 days after the operation revealed another subdural empyema in the le ...19911681835
percutaneous drainage of lung abscess in a malnourished child. 19911676504
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