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hydrogenase measurement with photochemically reduced methyl viologen.methyl viologen was reduced photochemically in the presence of proflavine and ethylenediaminetetraacetic acid. the reduced methyl viologen was oxidized by hydrogenase from vibrio succinogenes. h(2) and oxidized methyl viologen were the products. hydrogenase activity was determined by spectrophotometric measurement of the disappearance of reduced methyl viologen at 600 nm. the extinction coefficient of reduced methyl viologen was determined and is 8.25 mm(-1) x cm(-1) at 600 nm. optimal condition ...19695781586
amino acid composition of ferredoxin and rubredoxin isolated from desulfovibrio gigas.the composition of ferredoxin and rubredoxin from desulfovibrio gigas was determined. ferrodoxin contained five cysteines, two methionines, and four irons. the rubredoxin was not unique.19695781581
a non-specific adenine nucleotide deaminase from desulfovibrio desulfuricans. 19695773435
intermediary formation of trithionate in sulfite reduction by a sulfate-reducing bacterium. 19695771706
[serological study of sulfate reducing bacteria (genus desulfovibrio) isolated in rumania]. 19685754602
spontaneous spheroplast formation by desulfovibrio aestuarii. 19685710267
a note on the production of radioactive sulphide by desulfovibrio desulfuricans. 19685702041
the amino acid sequence of cytochrome c3 from desulfovibrio vulgaris. (n.c. i.b. 8303). 19685685884
the stereospecificity of the citrate synthase in sulfate-reducing and photosynthetic bacteria. 19685680354
purification and properties of hydrogenases of different origins. 19685650411
isolation from aspergillus nidulans, of a protein catalyzing the reduction of sulfite by reduced viologen dyes. 19675589532
thiosulfate reductase of desulfovibrio vulgaris.the thiosulfate reductase of desulfovibrio vulgaris has been purified and some of its properties have been determined. only one protein component was detected when the purified enzyme was subjected to polyacrylamide gel electrophoresis at ph values of 8.9, 8.0, and 7.6. in the presence of h(2), the enzyme, when coupled to hydrogenase and with methyl viologen as an electron carrier, catalyzed the reduction of thiosulfate to hydogen sulfide. the use of specifically labeled (35)s-thiosulfate reveal ...19715573735
purification and properties of a hydrogenase from desulfovibrio vulgaris.the soluble hydrogenase of desulfovibrio vulgaris was purified and some of its properties are described. the molecular weight was determined for the enzyme by sedimentation equilibrium (45,400) and amino acid analysis (44,800). the hydrogenase appears to be a loosely coiled molecule or to have a high axial ratio, which is reflected in an unusually low sedimentation coefficient (2.58s) and a low diffusion coefficient (d 5.85). the molecular weight of the hydrogenase (41,000), as calculated by the ...19715541010
nitrogen fixation by sporulating sulphate-reducing bacteria including rumen strains. 19705500022
nitrogen fixation by sulphate-reducing bacteria. 19705489063
solubilization, purification and properties of particulate hydrogenase from desulfovibrio vulgaris. 19705484445
phosphorylation coupled to oxidation of hydrogen with fumarate in extracts of the sulfate reducing bacterium, desulfovibrio gigas. 19705477217
role of thiosulfate in bisulfite reduction as catalyzed by desulfovibrio vulgaris.studies with (35)s-labeled substrates were conducted to investigate the pathway involved in the reduction of sulfite to sulfide by cell-free extracts of the sulfate-reducing organism desulfovibrio vulgaris. the results showed that accumulation of thiosulfate occurred when crude extracts were incubated under appropriate conditions with sulfite as substrate. with labeled sulfite as substrate, thiosulfate with equal distribution of radioactivity in both sulfur atoms was formed. when the rates of fo ...19705474884
malate dismutation by desulfovibrio. 19705469567
influence of sodium chloride on inhibition of desulfovibrio by a surfactant. 19705444097
c-type cytochromes of desulfovibrio vulgaris. amino acid composition and end groups of cytochrome c553. 19705443704
enzymes involved in the metabolism of thiosulfate by thiobacillus thioparus. ii. properties of adenosine-5'-phosphosulfate reductase. 19705438322
chemical study of two flavodoxins extracted from sulfate reducing bacteria. 19705437345
optical rotatory properties of the cytochromes c3 from three species of desulfovibrio. 19705436142
amino acid composition, heme content, and molecular weight of cytochrome c3 of desulfovibrio desulfuricans and desulfovibrio vulgaris. 19705436141
[study of dicarboxylic acid and pyruvate metabolism in sulfate-reducing bacteria. ii. electron transport; final acceptors]. 19705430708
reductant-activation of inorganic pyrophosphatase: an atp-conserving mechanism in anaerobic bacteria. 19705422601
a flavin-sulfite adduct as an intermediate in the reaction catalyzed by adenylyl sulfate reductase from desulfovibrio vulgaris. 19705421934
menaquinone-6 in the strict anaerobes desulfovibrio vulgaris and desulfovibrio gigas. 19705418698
menaquinone (mk-6) in the sulfate-reducing obligate anaerobe, desulfovibrio. 19705412037
isolation and characterization of sulfate-reducing bacteria from various marine environments. 19695384625
serine biosynthesis in desulfovibrio desulfuricans.cell-free extracts of desulfovibrio desulfuricans possess enzymes which catalyze the synthesis of serine from 3-phosphoglycerate via the intermediates phosphohydroxypyruvate and phosphoserine.19695370284
[sulfate-reducing bacteria (genus desulfovibrio) isolated from dental caries in humans]. 19695344220
classification of desulfovibrio species, the nonsporulating sulfate-reducing bacteria. 19665342518
recent advances in the study of the sulfate-reducing bacteria. 19655322044
atp generation by electron transport in desulfovibrio desulfuricans. 19705312617
deoxyribonucleic acid base composition of species of klebsiella, azotobacter and bacillus. 19695309904
[sulfate reducing vibrios and biological corrosion]. 19715161997
some observations on growth and hydrogen uptake by desulfovibrio vulgaris. 19715132464
physiological and chemical properties of a reductant-activated inorganic pyrophosphatase from desulfovibrio desulfuricans. 19715132274
purification of the enzyme reducing bisulfite to trithionate from desulfovibrio gigas and its identification as desulfoviridin. 19715128167
biosynthesis of dimethylarsine by methanobacterium. 19715126942
amino- and carboxyl-terminal amino acid sequences of the peptostreptococcus eisdenii and clostridium pasteurianum flavodoxins. 19715126921
a re-examination of desulfovibrio africanus. 19715126869
purification and properties of cytochrome c 3 of desulfovibrio vulgaris, miyazaki. 19715122658
a method for the electrophoretic characterization of sulfite reductases in crude preparations from sulfate-reducing bacteria using polyacrylamide gels. 19725084357
copper-molybdenum interactions with the sulfate-reducing system in rumen microorganisms. 19725059042
cytochromes and other pigments of dissimilatory sulphate-reducing bacteria. 19725052039
evidence for the presence of a b-type cytochrome in the sulfate-reducing bacterium desulfovibrio gigas, and its role in the reduction of fumarate by molecular hydrogen. 19725047130
c-type cytochromes of desulfovibrio vulgaris. the primary structure of cytochrome c 553. 19725038698
[purification and properties of a rubredoxin isolated from desulfovibrio vulgaris (ncib 8303)]. 19725031158
regulation of the reduction of sulfite and thiosulfate by ferredoxin, flavodoxin and cytochrome cc' 3 in extracts of the sulfate reducer desulfovibrio gigas. 19725017697
guanine plus cytosine contents of the deoxyribonucleic acids of some sulfate-reducing bacteria: a reassessment.guanine plus cytosine (gc) contents of the deoxyribonucleic acids of desulfovibrio and desulfotomaculum have been used as a basis for classification. some of these data have been incorrectly calculated, resulting in errors of as much as 5% gc. this situation has been corrected by a reanalysis of existing data and by the contribution of new data.19725011245
cytochrome c 3 . a class of electron transfer heme proteins found in both photosynthetic and sulfate-reducing bacteria. 19715003700
purification and properties of cytochrome c 3 of desulfovibrio salexigens. 19705001325
[effects of electric currents on cultures enriched with sulfate-reducing bacteria]. 19704990754
requirement for coenzyme a in the phosphoroclastic reaction of anaerobic bacteria.various bacteria which degrade pyruvate by the phosphoroclastic reaction were examined with respect to the role of coenzyme a (coa) in this reaction. the strictly anaerobic bacteria, which cleave pyruvate by the phosphoroclastic reaction characteristic of clostridia, required catalytic levels of coa for the co(2)-pyruvate exchange and acetoin-forming portions of the phosphoroclastic reaction. these reactions were reversibly inhibited by the coa analogue, desulfo-coa. in contrast, using cell-free ...19694982893
formate: cytochrome oxidoreductase of desulfovibrio vulgaris. 19694982127
electrophoretic and immunological differences between the cytochrome c3 of desulfovibrio desulfuricans and that of desulfovibrio vulgaris.the cytochrome c(3) of desulfovibrio desulfuricans and that of d. vulgaris were purified to homogeneity as judged by disc gel electrophoresis and by ultracentrifugation. both cytochromes had an oxidation-reduction potential of -205 +/- 5 mv at ph 7.0 and showed characteristic absorption bands at 525 and 553 nm in the reduced state. the molecular weights of the two cytochromes (calculated from sedimentation and diffusion data) were similar, with values of 13,500 to 14,300 for d. desulfuricans and ...19694981060
lysis of desulfovibrio vulgaris by ethylenediaminetetraacetic acid lysozyme. 19684971889
[purification and properties of a flavoprotein intervening in the reduction of sulfite by desulfovibrio gigas]. 19674964616
correlation of maximal growth temperature and ribosome heat stability. 19674962250
the amino acid sequence of ferredoxin from the sulfate reducing bacterium, desulfovibrio gigas. 19714946273
sulfate-reducing bacterium with unusual morphology and pigment content.a dissimilatory sulfate-reducing bacterium was isolated which differed in morphology and pigment content from previously described species. the organism was mesophilic, obligately anaerobic, gram-negative, nonsporulating, long, and slender with one polar flagellum. whole cells fluoresced red at neutral ph when excited with light at 365 nm owing to the presence of a pink pigment. desulfoviridin was present. reduced minus oxidized spectra of whole cells showed peaks in the position of a c-type cyt ...19714929856
the evaluation of media used to enumerate sulphate reducing bacteria. 19704923562
an apparatus for the continuous cultivation of sulfate-reducing bacteria and its application to geomicrobiological purposes. 19704914543
[morphology of sulfate reducing bacteria (genus desulfovibrio) isolated in rumania]. 19684912629
comparative studies of bacterial hydrogenase. 19694898625
[laboratory and clinical experiments of gentamicin with special reference to in vitro antibacterial activity, disc-sensitivity test and body fluid concentration assay methods]. 19684887885
[study of bacterial nitrate reductases a and b: methods]. 19684870325
corrosion of iron and formation of iron phosphide by desulfovibrio desulfuricans. 19684868375
separation of the apoprotein and reconstitution of the holoprotein from the long-lived intermediate in bacterial bioluminescence. 19744831059
reduction of sulphite to sulphide catalysed by desulfoviridin from desulfovibrio gigas. 19744824902
[sulfate-reducing bacteria from the petroleum strata of apsheron]. 19734791529
[absence of nitrogen release by methanosarcina]. 19734790727
a new strain of desulfovibrio gigas isolated from a sewage plant. 19734775730
[trophic relations between methanosarcina and its satellites]. 19734769917
a four-iron ferredoxin from desulfovibrio desulfuricans.ferredoxin from desulfovibrio desulfuricans was isolated, purified and crystallized. it contains four iron atoms and four sulphido or ;acid-labile' sulphur atoms for a molecule of 6000 daltons. the absorption spectrum in the u.v.-visible region and the electron-paramagnetic-resonance signals of the reduced protein are similar to those observed for other four-iron ferredoxins. the amino acid composition is different from that of desulfovibrio gigas ferredoxin. the redox potential of -0.33v at ph7 ...19734748838
isolation of assimilatroy- and dissimilatory-type sulfite reductases from desulfovibrio vulgaris.bisulfite reductase (desulfoviridin) and an assimilatory sulfite reductase have been purified from extracts of desulfovibrio vulgaris. the bisulfite reductase has absorption maxima at 628, 580, 408, 390, and 279 nm, and a molecular weight of 226,000 by sedimentation equilibrium, and was judged to be free of other proteins by disk electrophoresis and ultracentrifugation. on gels, purified bisulfite reductase exhibited two green bands which coincided with activity and protein. the enzyme appears t ...19734725615
the amino acid sequence of desulfovibrio vulgaris flavodoxin. 19734717757
isolation of a new pigment, desulforubidin, from desulfovibrio desulfuricans (norway strain) and its role in sulfite reduction.a new pigment, desulforubidin, that has sulfite reducing activity, has been purified from extracts of the norway strain of desulfovibrio desulfuricans, which lacks desulfoviridin.19734717523
a comparison of the electrophoretic properties of the atp-sulfurylases, aps-reductases, and sulfite reductases from cultures of dissimilatory sulfate-reducing bacteria. 19734697263
pyruvate-supported acetylene and sulfate reduction by cell-free extracts of desulfovibrio desulfricans. 19734693479
biochemical studies on sulfate-reducing bacteria. xi. purification and some properties of sulfite reductase, desulfoviridin. 19724644321
studies of flavin-protein interaction in flavoproteins using protein fluorescence and circular dichroism. 19724622437
[disruption of the integrity of the cytoplasmic membrane of microbial cells by surface-active compounds]. 19724598682
siroheme and sirohydrochlorin. the basis for a new type of porphyrin-related prosthetic group common to both assimilatory and dissimilatory sulfite reductases. 19734583265
a comment to the concept on the role of nitrate fermentation and nitrate respiration in an evolutionary pathway of energy metabolism. 19734581217
[microbiological preparation of mud substances used in pelotherapy]. 19724538492
redox potentials of certain vitamins k: implications for a role in sulfite reduction by obligately anaerobic bacteria.redox potentials of a menaquinone (mk-6), isolated in earlier researches from two species of the obligately anaerobic genus, desulfovibrio, as well as two other vitamins k(2)-menaquinones (mk-5) and (mk-9)- have been determined polarographically. the measurements have been validated by determination of redox potentials of 1,4-naphthoquinone and vitamin k(1) which agree with published potentiometric values. e(m7) for menaquinone (mk-6) is -0.067 +/- 0.010 v. redox potentials calculated for termin ...19744521797
the binding of riboflavin-5'-phosphate in a flavoprotein: flavodoxin at 2.0-angstrom resolution.the crystal structure of the oxidized form of flavodoxin from desulfovibrio vulgaris has been studied at 2.0-a resolution, and a detailed description of the region around the flavin mononucleotide binding site is now available. the flavin is between a tyrosine group, roughly parallel to it on one side, and a tryptophan, about 45 degrees from being parallel, on the other side. the two carbonyl groups and two nitrogen atoms of the flavin are hydrogen bonded to the peptide chain of the protein, whi ...19734521211
structure of the radical form of clostridial flavodoxin: a new molecular model.interpretation of a new electron-density map at 3.25-a resolution has led to a somewhat revised model for the free radical (semiquinone) structure of flavodoxin from clostridium mp. although the general conformation of the molecule is very similar to that of oxidized desulfovibrio vulgaris flavodoxin, flavin mononucleotide-protein interactions are not identical in the two flavodoxins. in the cl. mp semiquinone molecule, the isoalloxazine ring appears to retain the essentially planar conformation ...19724508314
structure of the oxidized form of a flavodoxin at 2.5-angstrom resolution: resolution of the phase ambiguity by anomalous scattering.flavodoxin from desulfovibrio vulgaris crystallizes in the oxidized form as well-formed, tetragonal bipyramids, space group p4(3)2(1)2, unit-cell parameters, a = b = 51.6 a, c = 139.6 a, 8 molecules per unit cell. the structure has been determined at 2.5-a resolution with phases based on a single isomorphous derivative. the phase ambiguity of a single derivative was resolved by use of anomalous scattering from the single-site sm(+3). the molecule has a five-strand pleated sheet core with two lon ...19724508313
sulfate reduction by a desulfovibrio species isolated from sheep rumen.several dissimilatory, sulfate-reducing bacteria were isolated from the rumen fluid of sheep fed purified diets containing sulfate. one isolate, strain d, was selected for characterization. this organism is a nonsporeforming, obligately anaerobic, mesophilic, nonmotile, gram-negative, straight rod. cell-free extracts show absorption maxima for cytochrome c(3) and desulfoviridin, characteristic of desulfovibrio. carbohydrates, as a sole carbon source, will support growth. lactate supports growth ...19744472525
evidence for the periplasmic location of hydrogenase in desulfovibrio gigas.hydrogenase has been found to be located in the periplasmic space of desulfovibrio gigas, and it is proposed that hydrogenase plays an important and specific role in interspecies hydrogen transfer.19744455692
[a new non-spore forming thermophilic organism, reducing sulfates, desulfovibrio thermophilus nov. sp]. 19744449494
phospholipid composition of desulfovibrio species.the phospholipids of desulfovibrio desulfuricans, norway strain, d. vulgaris, and d. gigas were examined in relationship to their qualitative and quantitative composition. d. desulfuricans and d. vulgaris exhibited an essentially identical phospholipid composition consisting of phosphatidylethanolamine, phosphatidylglycerol, cardiolipin, and lysophosphatidylserine. phosphatidylserine (10.9%) was present in d. desulfuricans but was not detected in d. vulgaris. d. gigas was found to contain only t ...19744436257
observations on the bisulfite reductase (p582) isolated from desulfotomaculum nigrificans.the bisulfite reductase (p582) from desulfotomaculum nigrificans was purified to homogeneity as judged by polyacrylamide gel electrophoresis. by colorimetric methods of analysis, the products of bisulfite reduction by this enzyme were determined to be trithionate, thiosulfate, and sulfide. of these, trithionate was consistently found to be the major product, whereas the latter two were formed in lesser quantities. when [(35)s]bisulfite was incorporated as substrate, no labeled sulfide was detect ...19744424068
temperature-jump studies of desulfovibrio vulgaris flavodoxin: kinetics of fmn binding and of reduction of semiquinone by methyl viologen. 19744420157
flavodoxin from the sulfate reducing bacterium desulfovibrio vulgaris. its structure at 2.5 a resolution. 19724405091
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