Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| phosphorylation of acinetobacter isocitrate lyase. | during growth on succinate, acinetobacter calcoaceticus contains two forms of the enzyme isocitrate dehydrogenase. addition of acetate to a lag-phase culture grown on succinate causes a dramatic increase in activity of form ii of isocitrate dehydrogenase and in isocitrate lyase. form ii of isocitrate dehydrogenase may be responsible for the partition of isocitrate between the tca cycle and the glyoxylate by-pass. this report describes the phosphorylation of the enzyme isocitrate lyase from a. ca ... | 1992 | 1731794 |
| genetic analysis of supraoperonic clustering by use of natural transformation in acinetobacter calcoaceticus. | dna within escherichia coli colonies carrying cloned acinetobacter calcoaceticus genes transforms mutant a. calocaceticus cells with high efficiency. therefore, e. coli colonies containing such cloned genes can be identified by replica plating onto a lawn of a. calcoaceticus mutant cells. transformation of a. calcoaceticus also facilitates gap repair and thus allows recovery of specified chromosomal segments in recombinant plasmids. these procedures were used to demonstrate the clustering of a. ... | 1992 | 1729209 |
| outer membrane permeability of acinetobacter calcoaceticus and its implication in antibiotic resistance. | in order to understand high and broad antibiotic resistance of acinetobacter calcoaceticus, the outer membrane permeability was studied. the permeability coefficients of zwitterionic cephalosporins in the intact cell outer membrane were 0.14-1.12 x 10(-4) cm3/min/mg protein. these values were two to seven times lower than the permeability coefficients of the same beta-lactams in the outer membrane of pseudomonas aeruginosa. the diffusion rates of carbapenems and zwitterionic cephalosporins into ... | 1991 | 1722802 |
| [gram-negative flora of horticulture products prevalently to be consumed fresh. ii, acinetobacter calcoaceticus]. | 1990 | 1711345 | |
| carbohydrate utilization in streptococcus thermophilus: characterization of the genes for aldose 1-epimerase (mutarotase) and udpglucose 4-epimerase. | the complete nucleotide sequences of the genes encoding aldose 1-epimerase (mutarotase) (galm) and udpglucose 4-epimerase (gale) and flanking regions of streptococcus thermophilus have been determined. both genes are located immediately upstream of the s. thermophilus lac operon. to facilitate the isolation of gale, a special polymerase chain reaction-based technique was used to amplify the region upstream of galm prior to cloning. the galm protein was homologous to the mutarotase of acinetobact ... | 1990 | 1694527 |
| [membrane-bound alanine aminopeptidase from acinetobacter calcoaceticus. 4. amino acid chloromethyl ketones as ph-dependent, substrate-derived inhibitors of cysteine protease]. | the alanine aminopeptidase (aap) from a. calcoaceticus is a metal-ion-dependent cysteine protease bound to the inner membranes of the cells. amino acid chloromethyl ketones are highly specific inhibitors of the enzyme. the aap is inhibited at ph 7.5 (ph-optimum) by mixed-type inhibition. a competitive inhibition type results if the ph value is decreased to 6.2. in contrast, the inhibition of the aap by substrate-derived chloromethyl ketones is of the irreversible type at ph 8.25. | 1991 | 1686704 |
| survival of bacteria under dry conditions; from a viewpoint of nosocomial infection. | the viability of non-sporing bacteria in a dry state was examined. the number of viable cells was determined at various time intervals, after inoculation onto cotton lint and a glass plate. viable cells of mycobacterium bovis were detected more than 2 months after inoculation, and this was the most resistant species to dry conditions among the bacteria tested. in the case of the gram-positive cocci tested and one species of gram-negative rods, acinetobacter calcoaceticus var. anitratus, the viab ... | 1991 | 1685507 |
| acinetobacter calcoaceticus bacteremia: analysis of 48 cases. | the medical records of 48 patients with acinetobacter calcoaceticus bacteremia over a recent two-year period were reviewed retrospectively to determine the epidemiologic and clinical features. there were 34 males and 14 females with a median age of 51 years. forty-six of the cases were hospital-acquired. the characteristics of these patients included residence in the intensive care unit, intubation and ventilatory support, preceding antibiotic therapy and intravascular catheterization. in vitro ... | 1991 | 1685177 |
| conjugational transfer of recombinant dna in cultures and in soils: host range of pseudomonas putida tol plasmids. | recombinant tol plasmid pwwo-eb62 allows pseudomonas putida to grow on p-ethylbenzoate. this plasmid can be transferred to other microorganisms, and its catabolic functions for the metabolism of alkylbenzoates are expressed in a limited number of gram-negative bacteria, including members of pseudomonad rrna group i and escherichia coli. transfer of the recombinant plasmid to erwinia chrysanthemi was observed, but transconjugants failed to grow on alkylbenzoates because they lost catabolic functi ... | 1991 | 1660698 |
| isolation, sequencing, and mutagenesis of the gene encoding cytochrome c553i of paracoccus denitrificans and characterization of the mutant strain. | the periplasmically located cytochrome c553i of paracoccus denitrificans was purified from cells grown aerobically on choline as the carbon source. the purified protein was digested with trypsin to obtain several protein fragments. the n-terminal regions of these fragments were sequenced. on the basis of one of these sequences, a mix of 17-mer oligonucleotides was synthesized. by using this mix as a probe, the structural gene encoding cytochrome c553i (cycb) was isolated. the nucleotide sequence ... | 1991 | 1657873 |
| in vitro and in vivo antibacterial activities of meropenem, a new carbapenem antibiotic. | the in vitro and in vivo antibacterial activities of meropenem were compared with those of imipenem, ceftazidime, flomoxef, cefuzonam and cefotiam. meropenem showed a broad antibacterial spectrum against clinical isolates of gram-positive and gram-negative bacteria. against gram-negative bacteria, with the exception of acinetobacter calcoaceticus, meropenem exhibited the most potent activity among the drugs tested. it inhibited all 330 strains of enterobacteriaceae at 0.78 mg/l. meropenem was se ... | 1992 | 1644010 |
| outer membranes of gram-negative bacteria are permeable to steroid probes. | the permeability of bacterial outer membranes was assayed by coupling the influx of highly hydrophobic probes, 3-oxosteroids, with their subsequent oxidation catalysed by 3-oxosteroid delta 1-dehydrogenase, expressed from a gene cloned from pseudomonas testosteroni. in salmonella typhimurium producing wild-type lipopolysaccharide, the permeability coefficients for uncharged steroids were 0.45 to 1 x 10(-5) cm s-1, and the diffusion appeared to occur mainly through the lipid bilayer domains of th ... | 1992 | 1640833 |
| characterization of transformation-deficient mutants of acinetobacter calcoaceticus. | three acinetobacter calcoaceticus transformation-deficient mutants, obtained by insertional mutagenesis with the nptll gene, have been characterized physiologically. one mutant (aac211) was found to be completely transformation deficient, while two others, aac213 and aac214, were severely impaired in transformation efficiency (100-1000 times lower than the wild type). the latter applied to both chromosomal as well as plasmid dna. analysis of the chromosomal dna fragments flanking the nptll gene ... | 1992 | 1630314 |
| murine antibody responses distinguish rochalimaea henselae from rochalimaea quintana. | rochalimaea henselae causes persistent bacteremia, bacillary angiomatosis, and parenchymal bacillary peliosis. detection of a specific antibody response to r. henselae infection may represent an alternative to cultivation as a means of diagnosis. we assessed the specificity of induced murine antibodies for antigens from r. henselae and the closely related species r. quintana. groups of cd-1 mice were inoculated with whole organisms of six strains of r. henselae and two of r. quintana. pre- and p ... | 1992 | 1629326 |
| infective endocarditis of a native valve due to acinetobacter: case report and review. | a case of community-acquired infective endocarditis of a native valve that was caused by acinetobacter calcoaceticus subspecies anitratus is presented. the previously reported cases are reviewed, and therapy for this disorder is discussed. the presence of a transient maculopapular rash involving the palms and soles but sparing the face is suggested as a possible early clinical clue to the diagnosis. native valve endocarditis caused by acinetobacter species is an acute, aggressive illness that is ... | 1992 | 1600019 |
| plasmid transformation of naturally competent acinetobacter calcoaceticus in non-sterile soil extract and groundwater. | the natural transformation of acinetobacter calcoaceticus bd413 (trpe27) was characterized with respect to features that might be important for a possible gene transfer by extracellular dna in natural environments. transformation of competent cells with chromosomal dna (marker trp+) occurred in aqueous solutions of single divalent cations. uptake of dna into the dnase i-resistant state but not the binding of dna to cells was strongly stimulated by divalent cations. an increase of transformation ... | 1992 | 1590708 |
| purification and characterization of an extracellular beta-lactamase produced by acinetobacter calcoaceticus. | a beta-lactamase was purified 430-fold from the culture supernatant of acinetobacter calcoaceticus by ion exchange chromatography on cm-sephadex and affinity chromatography on phenylboronic-acid-agarose. the purified enzyme was homogeneous as judged by sds-page, and was characterized with respect to molecular mass (38 and 41 kda by gel filtration on sephadex g-75 and sds-page, respectively), ph optimum (ph 7.0), temperature optimum (45 degrees c) and isoelectric point (9.3). the beta-lactamase s ... | 1992 | 1527494 |
| microbial contamination of enteral feeding solution and its prevention. | in an investigation of microbial contamination of enteral feeding solutions, all 22 residual solutions obtained immediately after administration were contaminated at concentrations of 10(3) to 10(6) viable counts/ml. major contaminants were glucose-nonfermenting gram-negative bacilli such as pseudomonas aeruginosa and acinetobacter calcoaceticus var anitratus. contamination seemed to have been caused by frequent reuse of bag-type containers and the infusion tubes connected to the bags, neither o ... | 1992 | 1524268 |
| purification and characterization of l-2,4-diaminobutyrate decarboxylase from acinetobacter calcoaceticus. | acinetobacter calcoaceticus atcc 23055 produces a large amount of 1,3-diaminopropane under normal growth conditions. the enzyme responsible, l-2,4-diaminobutyrate (daba) decarboxylase (ec 4.1.1.-), was purified to electrophoretic homogeneity from this bacterium. the native enzyme had an m(r) of approximately 108,000, with a pi of 5.0, and was a dimer composed of identical or nearly identical subunits with apparent m(r) 53,000. the enzyme showed hyperbolic kinetics with a km of 1.59 mm for daba a ... | 1992 | 1512577 |
| co-existence of beta-lactamase and penicillin acylase in bacteria; detection and quantitative determination of enzyme activities. | twenty-six bacteria were examined for the presence of penicillin acylase and beta-lactamase. a copper reducing assay, which was sensitive in the analytical range 2-20 micrograms/ml, was used for determination of penicilloates and a fluorescamine assay was used to determine 6-aminopenicillanic acid concentrations when both substances were produced by the action of the enzymes on a single substrate. seventeen bacteria contained beta-lactamases, six contained penicillin acylases and four contained ... | 1992 | 1512174 |
| purification of a periplasmic insulin-cleaving proteinase from acinetobacter calcoaceticus. | cells of acinetobacter calcoaceticus contain a constitutive periplasmic metalloproteinase showing similar properties as the periplasmic metalloproteinase of escherichia coli. the periplasmic proteinase of a. calcoaceticus was purified, starting from periplasm, by ammonium sulfate precipitation, hydrophobic interaction chromatography and chromatofocusing up to the homogeneity of the enzyme in sds-electrophoresis with a yield of 6.7% and a purification factor of 417. the enzyme has a molecular mas ... | 1992 | 1510571 |
| [antimicrobial activities of ceftazidime on fresh clinical isolates]. | antimicrobial activity of ceftazidime (caz) was compared with those of other cephem antibiotics against clinically isolated strains sent to us by medical institutions throughout japan in 1989 and 1991. those strains separated and identified from samples collected from patients with various infections were also examined, and the following results were obtained. 1. the results suggested that, compared with reports of studies conducted with clinical isolates in early 1980's, mic90 of caz in 1991 we ... | 1992 | 1494227 |
| in vitro and in vivo activities of dq-2556 and its mode of action. | dq-2556 is a recently developed cephalosporin with a broad spectrum of antibacterial activity against both gram-positive and gram-negative bacteria. its in vitro activity was roughly comparable to those of cefuzonam and cefpirome and greater than those of ceftazidime, cefepime, and cefclidin against gram-positive bacteria. against gram-negative bacteria, dq-2556 showed almost the same activity as those shown by cefpirome and cefepime. the activity was largely unaffected by culture medium ph or t ... | 1992 | 1482128 |
| characterization of a high-affinity iron transport system in acinetobacter baumannii. | analysis of a clinical isolate of acinetobacter baumannii showed that this bacterium was able to grow under iron-limiting conditions, using chemically defined growth media containing different iron chelators such as human transferrin, ethylenediaminedi-(o-hydroxyphenyl)acetic acid, nitrilotriacetic acid, and 2,2'-bipyridyl. this iron uptake-proficient phenotype was due to the synthesis and secretion of a catechol-type siderophore compound. utilization bioassays using the salmonella typhimurium i ... | 1992 | 1447137 |
| analysis of polar lipids from some representative enterobacteria, plesiomonas and acinetobacter by fast atom bombardment-mass spectrometry. | fast atom bombardment-mass spectrometry (fab-ms) was used to analyse lipid extracts of bacteria to assess its usefulness for analysing anionic phospholipids of potential chemotaxonomic value. the following micro-organisms were tested: acinetobacter calcoaceticus, acinetobacter sp., citrobacter freundii, enterobacter cloacae (2 strains), escherichia coli (3 strains), hafnia alvei, klebsiella oxytoca, klebsiella pneumoniae, morganella morganii, plesiomonas shigelloides, proteus mirabilis (3 strain ... | 1992 | 1447059 |
| long-chain alcohol and aldehyde dehydrogenase activities in acinetobacter calcoaceticus strain ho1-n. | three alcohol dehydrogenases have been identified in acinetobacter calcoaceticus sp. strain ho1-n: an nad(+)-dependent enzyme and two nadp(+)-dependent enzymes. one of the nadp(+)-dependent alcohol dehydrogenases was partially purified and was specific for long-chain substrates. with tetradecanol as substrate an apparent km value of 5.2 microm was calculated. this enzyme has a pi of 4.5 and a molecular mass of 144 kda. all three alcohol dehydrogenases were constitutively expressed. three aldehyd ... | 1992 | 1402794 |
| ribotyping of the acinetobacter calcoaceticus-acinetobacter baumannii complex. | the acinetobacter calcoaceticus-acinetobacter baumannii complex consists of four genotypically distinct but phenotypically very similar bacterial species or dna groups: a. calcoaceticus (dna group 1), a. baumannii (dna group 2), unnamed dna group 3 (p. j. m. bouvet and p. a. d. grimont, int. j. syst. bacteriol. 36:228-240, 1986), and unnamed dna group 13 (i. tjernberg and j. ursing, apmis 97:595-605, 1989). because strains in this complex cause nosocomial outbreaks, it is important to be able to ... | 1992 | 1383266 |
| cloning and nucleotide sequences of the acci restriction-modification genes in acinetobacter calcoaceticus. | the genes of the acci restriction-modification system specific for gt(a/c) (g/t)ac were cloned from the chromosomal dna of acinetobacter calcoaceticus, and their nucleotides sequenced. the restriction and modification genes coded for polypeptides with calculated molecular weights of 42,494 and 63,078, respectively. both the enzymes were coded by the same dna strand and the restriction gene was upstream of the methylase gene, separated by 2 bp. the restriction gene was significantly expressed in ... | 1991 | 1368703 |
| inducible high-level expression of heterologous genes in bacillus megaterium using the regulatory elements of the xylose-utilization operon. | we have constructed a shuttle plasmid for bacillus megaterium and escherichia coli that contains the promoter and repressor gene of the b. megaterium-borne operon for xylose utilization. a polylinker downstream of the promoter allows versatile cloning of genes under its transcriptional control. we have placed gdha (encoding glucose dehydrogenase) from b. megaterium, lacz (encoding beta-galactosidase) from e. coli, mro (encoding mutarotase) from acinetobacter calcoaceticus, and human puk (encodin ... | 1991 | 1367576 |
| natural transformation in acinetobacter calcoaceticus. | acinetobacter calcoaceticus is a metabolically versatile microorganism that is naturally competent for dna uptake and incorporation. we have exploited the natural state of competency for studies involving the cloning, organization and expression of genes encoding catabolic enzymes. a. calcoaceticus is able to take up, at high efficiency, genetically engineered dna, incorporate the dna and stably maintain and express the dna. sequence analysis of cloned a. calcoaceticus dna reveals a great deal o ... | 1990 | 1366417 |
| biotransformation of alkyl and aryl carbonates. microbial degradation. | an enriched mixed culture was successfully grown on model alkyl and aryl carbonates. these compounds were degraded by microorganisms at different rates. p-chlorophenyl-2-octyl carbonate and p-nitrobenzyl-2-octyl carbonate were metabolized through the formation of p-chlorophenol and p-nitrobenzyl alcohol respectively. a strain of acinetobacter calcoaceticus isolated from the mixed culture utilized phenyl-2-octyl carbonate by an intracellular hydrolase to phenol and 2-octanol which were further me ... | 1990 | 1366389 |
| epidemiological role of arthropods detectable in health facilities. | a total of 161 arthropod specimens were collected from 55 sites in a health care facility during july and september 1990. of the 116 bacterial isolates obtained from their body surfaces 6% were from parasites (mosquitoes), 59% from eusynanthropic arthropods (tenebrionid beetles, flies, german cockroaches, wasps), 16% from hemisynanthropic arthropods (ants, spiders) and 19% from occasionally encountered insects (non-biting midges, moths, beetles). most (88%) of the isolated bacteria were gram-neg ... | 1992 | 1350604 |
| in vitro and in vivo antibacterial activities of am-1155, a new 6-fluoro-8-methoxy quinolone. | am-1155 is a new quinolone with a wide spectrum of antibacterial activity against various bacteria including anaerobes and mycoplasma pneumoniae. am-1155 was 2- to 16-fold more active than ciprofloxacin and ofloxacin against staphylococcus aureus including methicillin-resistant strains, staphylococcus epidermidis, streptococcus pneumoniae, and enterococcus faecalis; its mics for 90% of strains tested were 0.10 to 0.78 micrograms/ml. the activity of am-1155 was comparable to that of ciprofloxacin ... | 1992 | 1332587 |
| cloning of an erwinia herbicola gene necessary for gluconic acid production and enhanced mineral phosphate solubilization in escherichia coli hb101: nucleotide sequence and probable involvement in biosynthesis of the coenzyme pyrroloquinoline quinone. | escherichia coli is capable of synthesizing the apo-glucose dehydrogenase enzyme (gdh) but not the cofactor pyrroloquinoline quinone (pqq), which is essential for formation of the holoenzyme. therefore, in the absence of exogenous pqq, e. coli does not produce gluconic acid. evidence is presented to show that the expression of an erwinia herbicola gene in e. coli hb101(pmcg898) resulted in the production of gluconic acid, which, in turn, implied pqq biosynthesis. transposon mutagenesis showed th ... | 1992 | 1325965 |
| acli, a new restriction endonuclease from acinetobacter calcoaceticus recognizing 5'-a decreases cgtt-3'. | 1992 | 1322531 | |
| plasmid profiles of acinetobacter and enterobacter species of hospital origin: restriction endonuclease analysis of plasmid dna and transformation of escherichia coli by r plasmids. | a total of 37 multi-resistant strains (20 acinetobacter calcoaceticus and 17 enterobacter cloacae) were isolated from patients of the intensive care units. all the isolates were examined for resistance to a battery of antimicrobial agents by the disk diffusion method. plasmid profiles and restriction endonuclease analysis of plasmid dna by ecor1 revealed the spread of one a. calcoaceticus and two e. cloacae endemic strains. transformation experiments on escherichia coli competent cells by three ... | 1992 | 1321240 |
| nucleotide sequence and structure of the klebsiella pneumoniae pqq operon. | a 6940 bp klebsiella pneumoniae chromosomal dna fragment, containing genes involved in pyrroloquinoline quinone (pqq) biosynthesis, was sequenced. six open reading frames, pqqa, pqqb, pqqc, pqqd, pqqe and pqqf were identified in the pqq operon, which coded for polypeptides of 2764 (23 amino acids), 33,464, 28,986, 10,436, 42,881 and 83,616 da, respectively. the transcription startpoint was mapped by primer extension analysis, upstream of pqqa, and promoter boxes could be identified. the gene pro ... | 1992 | 1313537 |
| a 24-amino-acid polypeptide is essential for the biosynthesis of the coenzyme pyrrolo-quinoline-quinone. | at least four genes are required for the biosynthesis of the coenzyme pyrrolo-quinoline-quinone (pqq) in acinetobacter calcoaceticus. the dna region where one of these genes was mapped codes for a polypeptide of only 24 amino acids. here we show that indeed this small peptide is essential for pqq synthesis. site-directed mutagenesis shows that at least one glutamate and one tyrosine residue of the polypeptide are essential for its function. | 1992 | 1310505 |
| antibacterial in vitro-activity of meropenem against 200 clinical isolates in comparison to 11 selected antibiotics. | the antimicrobial activity of meropenem, a new parenteral carbapenem, was tested in vitro by an agar dilution method against 200 clinical isolates (gram-negative/positive aerobes and anaerobes). meropenem was compared with imipenem, ceftazidime, cefotaxime, piperacillin, ciprofloxacin, gentamicin; and metronidazole, cefoxitin, chloramphenicol, clindamycin, vancomycin when appropriate. meropenem and imipenem exhibited an extended spectrum of activity with low minimal inhibitory concentrations (mi ... | 1992 | 1303691 |
| a simultaneous bilateral attack of infectious corneal ulcers in an extended-wear soft contact lens wearer: a case report. | a case study was made of a 23-year-old female with simultaneous bilateral infectious corneal ulcers related to extended-wear soft contact lenses. cultures revealed pseudomonas aeruginosa and acinetobacter calcoaceticus. multiple high risk factors were combined, including extended-wear, poor lens care, bilateral alternate wearing, and smoking. amphetamine addiction may also have played a role. after treatment, a visual acuity of 20/20 was achieved in both eyes by the use of rigid gas-permeable co ... | 1992 | 1296043 |
| leprosy in hiv-positive and syphilitic young paraguayan man. | in a borderline lepromatous patient a positive serology for syphilis and hiv 1 has been detected. the patient also had an urinary tract infection with acinetobacter calcoaceticus. in paraguay up till now no leprosy cases infected with hiv have been reported. | 1992 | 1293911 |
| protocatechuate 3, 4-dioxygenase from acinetobacter calcoaceticus. | protocatechuate 3,4-dioxygenase (pcd) from p-hydroxybenzoate-induced cells of acinetobacter calcoaceticus was purified by heat and protamine sulfate treatment, ammonium sulfate fractionation, deae-cellulose, and sephadex g-200 column chromatography. the enzyme appears to be homogeneous by ultracentrifugation and acrylamide gel electrophoresis. this is the first report of pcd purified from acinetobacter. for comparison, crystalline pseudomonas pcd was also obtained. the enzymes from acinetobacter ... | 1976 | 1252412 |
| [effect on growing conditions on protein content and amino acid composition of acinetobacter calcoaticus]. | the protein content and amino acid composition of acinetobacter calcoaceticus were studied as dependent on the stages of development and different hydrocarbon sources. the protein content was always the greatest at the logarithmic stage and decreased approaching the stationary stage. when cultivating on different hyrocarbon sources only an insignificant differedce in the content of protein was observed. a relative amino acid composition of ac. calcoaceticus remained practically constant during t ... | 1975 | 1209770 |
| regulation of growth of acinetobacter calcoaceticus ncib8250 on l-mandelate in batch culture. | batch culture of acinetobacter calcoaceticus in l-mandelate- or phenylglyoxylate-salts medium showed an unusual non-exponential pattern unless the inoculum had been grown on benzyl alcohol. there were transient accumulations of benzaldehyde and benzyl alcohol caused by the limitation of l-mandelate oxidation by low activities of benzaldehyde dehydrogenase and the diversion of reducing power to the formation of benzyl alcohol. in vivo enzymic activities were estimated from patterns of substrate u ... | 1975 | 1206374 |
| [inhibiton of isocitrate dehydrogenase and isocitrate lyase from acinetobacter calcoaceticus by acids of the citrate and glyoxylate cycle]. | acinetobacter calcoaceticus contains two forms of nadp+-dependent isocitrate dehydrogenases differing, among others, by their molecular weights and regulatory properties. the regulation of the high-molecular form of isocitrate dehydrogenase and of isocitrate lyase by organic acids, either belonging or related to the citrate and glyoxalate cycle, is investigated. while alpha-ketoglutarate and oxalacetate competitively inhibit the isocitrate dehydrogenase against ds-isocitrate, glyoxylate and pyru ... | 1975 | 1199592 |
| metabolism of monofluorobenzoates by acinetobacter calcoaceticus n.c.i.b. 8250. formation of monofluorocatechols. | none of the monofluorobenzoates serves as sole source of carbon and energy for growth of acinetobacter calcoaceticus but all can contribute to growth on other substrates. the monofluorobenzoates are oxidised by bacteria pre-induced for benzoate oxidation and can themselves induce the appropriate enzymes. the initial products of oxidation have been separated and identified by gas-liquid chromatography. 2-flurobenzoate is oxidised to catechol, fluoride and 3-fluorocatechol; 3-fluorobenzoate gives ... | 1975 | 1182155 |
| beta-ketoadipate enol-lactone hydrolases i and ii from acinetobacter calcoaceticus. | beta-ketoadipate enol-lactone hydrolase catalyzes a common step in the utilization of protocatechuate and cis,cis-muconate by bacteria. either of the two compounds elicits the synthesize of an enol-lactone hydrolase in acinetobacter. the enol-lactone hydrolase that is induced by each compound was purified, and the properties of the proteins were compared. both enzymes appear to be dimers with molecular weights of approximately 25,000. the amino acid compositions of the enzymes differ, and the tw ... | 1975 | 1158871 |
| the occurrence of twitching motility among gram-negative bacteria. | almost 1000 strains representing well above 50 different species or groups of gram-negative bacteria were examined for twitching motility. this kind of motility was mainly found in strictly aerobic cocci and rods (viz. acinetobacter calcoaceticus, moraxella spp., neisseria gonorrhoeae, n. meningitidis, pseudomonas spp., phenon 3 of thornley that is closely related to acinetobacter, and marine, yellow-pigmented rods), but also in the facultative eikenella corrodens and in anaerobic strains presum ... | 1975 | 1155115 |
| correlation between twitching motility and possession of polar fimbriae in acinetobacter calcoaceticus. | negatively stained preparations of 16 strains of acinetobacter calcoaceticus were examined in the electron microscope. five of the strains did and 11 of the strains did not exhibit twitching motility. two of the non-twitching strains were substrains of twitching wild-type strains. all twitching strains were found to possess fimbriae with a diameter of approximately 50 a. these fimbriae were shown to be of polar origin except in one strain where, for technical reasons, the origin could not be det ... | 1975 | 1155109 |
| activity of minocycline against acinetobacter calcoaceticus var. anitratus (syn. herellea vaginicola) and serratia marcescens. | the activity of minocycline and tetracycline against 23 isolates of acinetobacter calcoaceticus var. anitratus (syn. herellea vaginicola) and 178 strains of serratia marcescens was determined by disk and microdilution methods. the results indicate that minocycline is highly active against this species of acinetobacter, all but one strain being inhibited by 0.007 mug of the antibiotic per ml. tetracycline was also active, though to a lesser degree, against a. calcoaceticus. of the 178 strains of ... | 1975 | 1099982 |
| common enterobacterial antigen in yersinia enterocolitica. | production of the common enterobacterial antigen (ca) by strains of yersinia enterocolitica (y.e.) serotypes 3 and 9 (winblad), by strains of 5 different y.e. serogroups (wauters) and various other bacteria was examined. antibody against ca was raised by immunization of rabbits with e. coli o 14. extract prepared from s. typhimurium was used for the sensitization of sheep erythrocytes with ca. absorption and haemagglutination inhibition experiments showed that ca could be detected in heat extrac ... | 1975 | 1098394 |
| rp4 fertility variants in acinetobacter calcoaceticus. | 1976 | 1026570 | |
| constitutivity of the mandelate enzymes in acinetobacter calcoaceticus, n.c.i.b. 8250, and its effect on the synthesis of benzyl alcohol dehydrogenase. | 1976 | 1022571 | |
| mandelate dehydrogenase with altered stereospecificity in mutant strains of acinetobacter calcoaceticus n.c.i.b. 8250. | 1976 | 1001725 | |
| regulation of growth of acinetobacter calcoaceticus ncib8250 on benzyl alcohol in batch culture. | formation of benzoate and catechol during oxidation of benzyl alcohol by washed suspensions of acinetobacter calcoaceticus ncib8250 confirmed earlier results indicating that this organism metabolizes benzyl alcohol via benzaldehyde, benzoate, and the 3-oxoadipate pathway. there was no evidence for feedback inhibition of benzyl alcohol dehydrogenase or benzaldehyde dehydrogenase ii. examination of growth curves and patterns of substrate utilization, as well as measurement of enzyme activities, sh ... | 1976 | 993779 |
| emergence of gentamicin-resistant bacteria: experience with tobramycin therapy of infections due to gentamicin-resistant organisms. | a computerized system for testing and surveillance of bacterial susceptibility to antibiotics was used in monitoring the emergence of gentamicin-resistant strains of aerobic and facultative gram-negative bacilli at massachusetts general hospital since the release of gentamicin for clinical use in 1971. during the period studied, there was a significant increase in the prevalence of gentamicin-resistant bacteria, particularly among pseudomonas, acinetobacter (herellea), and proteus and, more rece ... | 1976 | 972284 |
| [isolation and characterization of rubredoxin from acinetobacter calcoaceticus]. | acinetobacter calcoaceticus growing on long-chain n-alkanes contains a soluble iron-sulfur protein, which corresponds in its properties to a rubredoxin. it was prepared from the 50000 x g supernatant of ultrasonically treated cells using ion exchange chromatography on deae cellulose and gel filtration on sephadex g-75. the isolated protein is pure electrophoretically, but yields two bands corresponding to molecular weights of 6000 and 12000 respectively. a content of 11 acidic against 6 basic am ... | 1976 | 970050 |
| purification and properties of l-asparaginase b from acinetobacter calcoaceticus. | l-asparaginase (l-asparagine amidohydrolase, ec 3.5.1.1) b from acinetobacter calcoaceticus has been purified by precipitation with streptomycin, chromatography on deae-cellulose and cm-cellulose, gel filtration on agarose and chromatography on phosphocellulose. the molecular weight of the enzyme was found to be 130 000. the enzyme was rather insensitive to ph changes between 7 and 9. the michaelis constant was 3-10(-3) m. hg2+, cu2+, and ni2+ as well as high ionic strength inhibited the activit ... | 1976 | 938683 |
| the fate of bacteria in frozen red cells. | units of blood were intentionally contaiminated with suspensions of either aerobacter aerogenes, escherichia coli, bacillus subtilis, enterobacter cloacae, pseudomonas aeruginosa, sarcina lutea, seratia marcesens, staphylococcus epidermidis, streptococcus faecalis, paracolabacterum aerogenoides (enterobacter hafriae), mima polymorpha or acinetobacter- calcoaceticus. when inoculation was made prior to glycerolization, the subsequent glycerolization, freezing, thawing, and deglycerolization result ... | 1976 | 936269 |
| regulation of enzyme synthesis in the tryptophan pathway of acinetobacter calcoaceticus. | in acinetobacter calcoaceticus the seven genes coding for the enzymes responsible for tryptophan synthesis map at three chromosomal locations. two three-gene clusters, one (trpgdc) specifying the small subunit of anthranilate synthase, phosphoribosyl transferase, and indoleglycerol phosphate synthase and the other (trpfba) specifying phosphoribosyl anthranilate isomerase and both tryptophan synthase subunits, are not linked to each other or to the trpe gene specifying the large anthranilate synt ... | 1976 | 931950 |
| [polarographic determination of oxygen uptake of acinetobacter calcoaceticus during incubation with n-alkanes and their derivatives]. | 1977 | 930122 | |
| [isolation and characterization of cell surface layers of acinetobacter calcoaceticus]. | 1977 | 930118 | |
| pseudobacteremia: false-positive blood cultures from mist tent contamination. | in the seven-month period from july 1975 through january 1976, 11 pediatric patients had acinetobacter calcoaceticus var. anitratus cultured from blood; this organism had not been isolated from pediatric patients in the previous six months. in 10 of 11 patients, only the first of two cultures was positive. all patients recovered uneventfully, although only two were treated with appropriate antibiotics. nine of 11 had been in mist tents at the time of the culture. mist cultured from one tent cont ... | 1977 | 888818 |
| [induction of nadp+-dependent aldehyde dehydrogenase in acinetobacter calcoaceticus by hydrocarbons]. | 1977 | 888459 | |
| sudden death due to waterhouse-friderichsen syndrome and purulent leptomeningitis caused by acinetobacter calcoaceticus (mima polymorpha). | a case of waterhouse-friderichsen syndrome presenting with purulent leptomeningitis causing sudden death is described. the causative agent, acinetobacter calcoaceticus (mina polymorpha), was determined by post-mortem microbiological examination. | 1977 | 883428 |
| in vitro synergism between carbenicillin and aminoglycosidic aminocyclitols against acinetobacter calcoaceticus var. anitratus. | acinetobacter caleoaceticus var. anitratus is a nonfermentative, gram-negative bacillus that has been demonstrated to cause severe infections, usually in hospitalized patients. since mild to moderate resistance of a. calcoaceticus to one or more aminoglycosidic aminocyclitols has been noted to occur, a study was undertaken to evaluate the activity of combinations of carbenicillin with either kanamycin, tobramycin, or gentamicin against 28 isolates of a. calcoaceticus obtained from clinical sourc ... | 1977 | 879749 |
| [oxidation of n-tetradecan-1-14c by cell-free extracts from acinetobacter calcoaceticus]. | 1977 | 878503 | |
| [utilization of trimethylammonium-compounds by acinetobacter calcoaceticus (author's transl)]. | the utilization of carnitine and carnitine derivatives (o-acylcarnitines, carnitine carboxylderivatives) and structure-related trimethylammonium-compounds (betaines and nitrogen-bases) by acinetobacter calcoaceticus was studied by means of the control of growth and the quantitative detection of metabolites. the strain grew only on l-carnitine, l-o-acylcarnitines, and gamma-butyrobetaine as the sole carbon sources. the utilization of these compounds and the growth correlated with the cleavage of ... | 1977 | 849100 |
| assimilatory nitrate reductase from acinetobacter calcoaceticus. | a soluble nitrate reductase from the bacterium acinetobacter calcoaceticus grown on nitrate has been characterized. the reduction of nitrate to nitrite is mediated by an enzyme of 96000 molecular weight that can use as electron donors either viologen dyes chemically reduced with dithionite or enzymatically reduced with nad(p)h, through specific diaphorases which utilize viologens as electron acceptors. nitrate reductase activity is molybdenumdependent as shown by tungstate antagonistic experimen ... | 1977 | 849099 |
| infections with acinetobacter calcoaceticus (herellea vaginicola): clinical and laboratory studies. | in a retrospective review of 53 patients, 58 episodes of infection due to acinetobacter calcoaceticus var. anitratus (herellea vaginicola) were studied. although the organism is widely distributed in nature, it is of relatively low virulence since colonization is more frequently noted than infection and since most infections occur in patients subjected to the epidemiologic pressures common to nosocomial, gram-negative bacillary infection: prior antibiotic therapy; instrumentation and manipulatio ... | 1977 | 846390 |
| the microbiology of evacuated blood collection tubes. | a collaborative study involving 20 clinical laboratories representing diverse geographical regions was organized to examine the microbiology of evacuated blooc collection tubes. overall, 14% of the 1433 tubes examined contained microorganisms representing a variety of bacteria and fungi. of note was the finding that nearly 9% of tubes with yellow closures, which are stated to be sterile by the manufacturers, contained bacteria, including pseudomonas aeruginosa, enterococci, serratia marcescens, ... | 1977 | 835941 |
| [infective situation in a cow barn contaminated with klebsiella mastitis]. | the infection situation in a four-row cow-house for 153 animals with a frequent occurrence of klebsiella mastitis was subject to a detailed analysis. the following results were obtained after two collections of blood and udder-quarter milk samples examined by the test-tube agglutination, gel precipitation, and bacteriological diagnosis methods: 1. streptococcus agalactiae was isolated from milk samples 28 and 31 times, pseudomonas aeruginosa 15 and 19 times, staphylococcus arueus 16 and 19 times ... | 1976 | 828991 |
| susceptibility of nonfermentative gram-negative bacilli to tobramycin. | there has been increasing interest in the pathogenic role of nonfermentative gram-negative bacilli in human infections. except for pseudomonas aeruginosa, the susceptibility pattern of these organisms to tobramycin has not been evaluated thoroughly. the activity of tobramycin, as compared with that of gentamicin, was tested by the serial broth dilution technique against 178 isolates of nonfermentative gram-negative bacilli obtained from various sources. p. aeruginosa, pseudomonas stutzeri, acine ... | 1976 | 823278 |
| the induction of mutants of acinetobacter calcoaceticus ncib8250 and their selection by vancomycin. | the mutagenic and lethal effects of n-methyl-n'-nitro-n-nitrosoguanidine (ntg), ethyl methanesulphonate (ems), ultraviolet light irradiation and near-ultraviolet light irradiation with 8-methoxypsoralen on the bacterium acinetobacter calcoaceticus ncib8250 were examined. the production of auxotrophic mutants was used as a measure of mutagenic efficiency. under appropriate conditions all four agents were mutagenic. ems and ntg although more effective than irradiation, did not cause such a high fr ... | 1975 | 812942 |
| in vitro susceptibility of acinetobacter calcoaceticus (herellea vagincola) to tetracycline and minocycline. | 1976 | 812659 | |
| mechanical ventilators as vehicles of infection. | twenty-five patients from the intensive care unit who had been subjected to tracheostomy and require mechanical respiration were studied. data are presented concerning the bacteriological controls performed on the tracheal exudates, various parts of the ventilators (engström model 200 and bennett pr-2), and the wright spirometer. the germs found were mainly pseudomonas, enterobacteriaceae germs and bacteria from the acinetobacter group. there was a high incidence of infection in the tracheostomi ... | 1975 | 811072 |
| susceptibility of acinetobacter calcoaceticus var. anitratus (herellea vaginicola) to minocycline. | the in vitro activity of minocycline against acinetobacter calcoaceticus var. anitratus (herellea vaginicola) was examined. all strains of a. anitratus were inhibited by minocycline at concentrations of 0.4 mug/ml or less. the strains studied were considerably more susceptible to minocycline than tetracycline. no difference between the susceptibility to minocycline and tetracycline was seen with escherichia coli, klebsiella pneumoniae, enterobacter and proteus sp. a regression line of the log mi ... | 1976 | 791084 |
| conversion of escherichia coli rna polymerase to a template independent enzyme. | preparations of rna polymerase (e.c.2.7.7.6) from uninfected escherichia coli, t4 infected escherichia coli, and acinetobacter calcoaceticus when centrifuged in sucrose gradients in the absence of magnesium ions gave rise to five peaks, all of which were able to form polymers from ribonucleoside 5'-triphosphates in the absence of template or primer. all of the peaks obtained from the escherichia coli enzyme appeared to contain the subunit alpha and beta and, in addition, polypeptides which appe ... | 1976 | 787928 |
| rp4-mediated conjugation in acinetobacter calcoaceticus. | the p class r factor rp4 was transferred from escherichia coli k12 to acinetobacter calcoaceticus. rp4 conferred similar levels of antibiotic resistance in a. calcoaceticus to those in the e. coli k12 donor. only slight instability of rp4 in a. calcoaceticus was detected. transfer of rp4 between strains of a. calcoaceticus was by conjugation and was accompanied by transfer of chromosomal genes. possible polarity of marker transfer was observed and linkage between a number of chromosomal markers ... | 1976 | 778327 |
| [bacteriemia due to acinetobacter calcoaceticus var. anitratus (author's transl)]. | a serious bacteremia caused by acinetobacter calcoaceticus var. anitratus could be observed. from the clinical point of view the most important symptoms were shivering, high tempeature up to 41 degrees c and clinical signs of acute hepatitis. there were pathological changes in the eeg. certain signs of an outlayer damage of the heart could be seem in the ecg. the pathogenic microorganism isolated from the blood culture media was classified. specific antibodies--agglutinines and precipitines--wer ... | 1978 | 723766 |
| [cytochrome composition of acinetobacter calcoaceticus]. | the qualitative and quantitative composition of cytochromes in intact cells of acinetobacter calcoaceticus and in particle fractions obtained from cells following ultrasonic treatment by differential centrifugation were studied using spectrophotometric methods. acinetobacter calcoaceticus contains cytochrome b, cytochrome o and low amounts of cytochrome d. both the absolute content of cytochrome b and o and the relative composition do not essentially vary with the carbon source used (hexadecane, ... | 1978 | 706933 |
| search for acinetobacter calcoaceticus subsp. anitratus: enrichment of fecal samples. | none of 50 healthy individuals and only 2 of 50 hospitalized patients (with acinetobacter anitratus elsewhere) yielded a. anitratus on enrichment from fecal samples. | 1978 | 701469 |
| chronic haematuria caused by acinetobacter calcoaceticus in a race horse. | 1978 | 676010 | |
| mutants of acinetobacter calcoaceticus ncib 8250 constitutive for the mandelate enzymes. | methods for isolating mutants of acinetobacter calcoaceticus ncib 8250 constitutive for the mandelate enzymes were compared. non-inducing substrates were not available. continuous culture gave no stable constitutive mutants. alternate culture in inducing (l-mandelate) and non-inducing (l-glutamate) media led to the isolation of mutants mesoconstitutive for l-mandelate dehydrogenase and phenylgloxylate carboxy-lyase, and hyperinducible for benzaldehyde dehydrogenase i. screening of possible anti- ... | 1978 | 670930 |
| acinetobacter calcoaceticus outbreak associated with peritoneal dialysis. | investigation of an outbreak of contamination of dialysis drainage fluid with acinetobacter calcoaceticus var. anitratus identified a previously unrecognized source for dialysis associated infections. over a 4-month period, 25 peritoneal dialysis treatments were administered to 13 hospital patients. of the 25 treatments for which culture results were available, 14 were associated with dialysis drainage fluid cultures positive for a. calcoaceticus. a water bath used to warm bottles of peritoneal ... | 1978 | 645702 |
| chromosome mapping in acinetobacter calcoaceticus. | rp4-mediated conjugation was used to map the loci of 23 different mutations on a circular linkage group in acinetobacter calcoaceticus ebf65/65. the resulting genetic map indicated that the chromosomal organization of a. calcoaceticus differed from that of members of the enteric group of bacteria and was similar to pseudomonas in showing an absence of clustering of functionally related genes. | 1978 | 632801 |
| utilization of oxalacetate by acinetobacter calcoaceticus: evidence for coupling between malic enzyme and malic dehydrogenase. | growth of acinetobacter calcoaceticus strain bd413 in malate-mineral medium resulted in the excretion of large quantities of oxalacetate. malate was virtually depleted by the time the cell density reached 60% of its final value; most of the remaining growth took place at the expense of oxalacetate. experiments in which oxalacetate was used as the initial substrate showed that pyruvate was not utilized until most of the oxalacetate disappeared. the generation time for growth on malate or oxalacet ... | 1978 | 627536 |
| equine myositis and septicemia caused by acinetobacter calcoaceticus infection. | myositis and septicemia caused by acinetobacter calcoaceticus were diagnosed in a mare. the infection was characterized clinically by ventral swelling and edema, diarrhea, listlessness, and rectal temperature of 39.4 c. the mare was treated symptomatically for 2 days but died on the 3rd day. conditions seen at necropsy were myositis, enteritis, typhlitis, colitis, and hepatitis. lymph nodes were moderately enlarged throughout the body. gross lesions in musculature were edema, scarring, petechiae ... | 1978 | 621183 |
| genetic analysis of acinetobacter calcoaceticus proline auxotrophs. | twenty-six acinetobacter calcoaceticus proline auxotrophs were isolated after ethyl methane sulfonate mutagenesis. studies using the efficient transformation system of this organism indicate that the mutations comprise therr genetically distinct groups. | 1978 | 618847 |
| [uptake of acetate by acinetobacter calcoaceticus]. | the uptake of acetate by intact nongrowing cells of acinetobacter calcoaceticus was studied in dependence on the c-source (acetate, n-alcanes, yeast extract, succinate, l-malate) and the growth phase. single kinetic parameters of acetate uptake were determined. the best acetate uptake was observed with cells cultivated with acetate as the only c-source. bacteria in the early growth phase were found to transfer acetate twice as fast as cells of the late logarithmic growth phase. the uptake of ace ... | 1977 | 614747 |
| [characteristics of growth and changes in the ultrafine structure of bacteria in the course of continuous cultivation on media containing ethanol]. | a large number of bacterial strains assimilating chemical ethanol has been isolated using an original technique. active growth of strains belonging to the genera pseudomonas and, particularly, acinetobacter was registered on mineral media containing ethanol. a mathematical model was constructed select a strain of acinetobacter calcoaceticus k-9 during its continuous cultivation on media containing ethanol. the model makes it possible to determine conditions for producing a present amount of the ... | 1977 | 600096 |
| some aspects of an epidemiological study of nosocomial infections due to acinetobacter calcoaceticus (herellea) featuring bacteriocin typing. | 1977 | 585613 | |
| critique: "some aspects of an epidemiological study of nosocomial infections due to acinetobacter calcoaceticus (herellea) featuring bacteriocin typing". | 1977 | 585596 | |
| room humidifiers as the source of acinetobacter infections. | twenty-four patients contracted systemic infections with acinetobacter calcoaceticus during a four-month period. unheated room humidifiers at the patients' bedsides were implicated as the source of infection in six. the outbreak was terminated with the removal of the humidifers. | 1977 | 576319 |
| chronic haematuria caused by acinetobacter calcoaceticus. | 1978 | 567878 | |
| [isolation and identification of acinetobacter calcoaceticus from clinical materials and it's clinial value in respiratory infectious diseases (author's transl)]. | 1977 | 559145 | |
| [a case of meningitis due to acinetobacter calcoaceticus]. | meningitis due to acinetobacter calcoaceticus is rare. these rare bacteria were isolated from culture fluid in patient resembling to neisseria meningitis with clinical and laboratory findings. the patient was treated with sensitive antibiotic. | 1979 | 555785 |
| [uptake of organic acids by acinetobacter calcoaceticus]. | 1979 | 543155 | |
| [structural features of acinetobacter calcoaceticus k-9 cells assimilating ethanol at different rates of medium dilution]. | 1979 | 530115 | |
| in vitro activity of three tetracycline antibiotics against acinetobacter calcoaceticus subsp. anitratus. | the in vitro activity of three tetracycline antibiotics against 127 strains of acinetobacter calcoaceticus (herella vaginicola) were compared. almost all strains were susceptible to minocycline and doxycycline, whereas most strains were resistant to tetracycline. | 1979 | 526013 |