Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| recovery study in mg-deficient rats given an organic source of mg. | recovery from mg deficiency was studied in rats given an organic source of mg derived from yeast (saccharomyces cerevisiae) cultured in an mg gluconate-enriched medium, as the only source of mg. magnesium was given either at a rate above the rda or as a supplement to the regular laboratory diet at the rate of 70 mg mg/100 g food, of which 40 mg mg was in the form of mgco3 and the remaining 30 mg mg was in the form of the organic product under study. the results showed a good degree of digestive ... | 1990 | 2400206 |
| [the metabolic efficiency of baker's yeast in an atherogenic diet]. | the effect of bakery compressed yeasts in the amounts of 6 and 12 g/100 g of the ration was studied in rats. it was revealed that 12 g of yeasts per 100 g of the atherogenic ration produced a hypocholesterolemic effect. a negative effect of this amount of the yeasts was manifest in the slow growth of the rats' body mass and in an increased ratio of the kidney/body mass. the yeasts amounts used did not protect the test animals from the kidney infiltration with lipids and cholesterol; 12 g of yeas ... | 1990 | 2378104 |
| a novel peroxisomal nonspecific lipid-transfer protein from candida tropicalis. gene structure, purification and possible role in beta-oxidation. | we have sequenced the nucleotides of the gene pox18 that encodes pxp-18, a major peroxisomal polypeptide inducible by oleic acid in the yeast candida tropicalis. pox18 had a single open reading frame of 127 amino acids. some 33% of the amino acid sequence of the predicted basic polypeptide (13,805 da), was identical to that of the nonspecific lipid-transfer protein (sterol carrier protein 2) from rat liver. pxp-18, purified to near homogeneity from isolated peroxisomes, had an amino-terminal seq ... | 1990 | 2364939 |
| mitochondrial translocation and processing of the precursor to the alpha-subunit of rat liver succinyl-coa synthetase. | succinyl-coa synthetase functions in the mitochondrial matrix as an alpha beta-dimer. its constitutive subunits are thus expected to be encoded in the nucleus and synthesized in the cytoplasm as precursors containing signal sequences for mitochondrial translocation. we have previously reported the isolation and sequence of a rat liver cdna clone (lambda scs19) that apparently encodes the cytoplasmic precursor to the alpha-subunit. here we report the preparation of mrna transcripts of this cdna i ... | 1990 | 2350494 |
| replication-linked histone acetylation in rat liver tissue is sensitive to alkylating agents. | the effect of alkylating agents on histone acetyltransferase (ec 2.3.1.48) activity and thymidine incorporation was investigated in benign and malignant proliferating rat liver tissue and compared with the effect in normal non-proliferating rat liver tissue. in both, benign and malignant proliferating tissue, but not in quiescent tissue, the histone acetylation is depressed by alkylating agents and this depression correlates with the inhibition of the thymidine incorporation. this effect suggest ... | 1990 | 2338138 |
| pharmaco-toxicological effects of acetaminophen in rodents. battery of tests to screen potential analgesic acetaminophen derivatives. | the pharmacological effects of acute oral administration of acetaminophen have been extensively evaluated in rodents. using this drug as reference compound we have also standardized several pharmaco-toxociological tests in order to select new analgesic-antipyretic acetaminophen derivatives. according to the results obtained in this work, the following battery of assays is proposed to screen these compounds: a) pharmacological: antipyretic activity against brewer's yeast-induced pyresis in rats; ... | 1990 | 2319838 |
| comparative toxicity and tissue retention of selenium in methionine-deficient rats fed sodium selenate or l-selenomethionine. | selenium (se) toxicity is known to be affected by level of intake of the mineral, but there are conflicting reports on the relative toxicities of the various chemical forms of se. we monitored se toxicity in rats fed torula yeast-based diets containing 0.1, 0.5 or 2.5 micrograms se/g of diet as either sodium selenate (na2seo4) or l-selenomethionine (semet). half the diets were supplemented to contain adequate dietary methionine (met). weights were monitored weekly for 6 (met-adequate) or 7 (met- ... | 1990 | 2313384 |
| comparison of the magnitude of the selenite-exchangeable metabolic pool and whole body endogenous selenium in adult rats. | the quantitative relationship between the size of the selenite-exchangeable metabolic pool (wse-emp) and whole body endogenous selenium (seend) was investigated in adult male rats. two experiments based on multiple labeling with stable isotopes were performed. one focused on short-term (7 d, expt. 1) and the other on long-term (60 d, expt. 2) relationships. rats were fed a torula yeast diet and water supplemented with [76se]selenite at 0.1 micrograms se/ml; the in vivo [74se]selenite tracer was ... | 1990 | 2313382 |
| the rat paw formalin test: comparison of noxious agents. | a comparison was made of the spontaneous nociceptive behaviors elicited by s.c. injection into the rat hind paw of the following 8 irritants: acetic acid, carrageenan, formalin, kaolin, platelet-activating factor, mustard oil (given topically), serotonin, and yeast. two distinct quantifiable behaviors indicative of pain were identified: flinching/shaking of the paw and hindquarters and licking/biting of the injected paw. these behaviors were prolonged and intense after formalin and acetic acid. ... | 1990 | 2308768 |
| biochemical mode of action and enantiomeric selectivity of sdz 89-485, a new triazole antimycotic. | sdz 89-485, a new triazole antimycotic agent, potently inhibited ergosterol biosynthesis in cells of candida. trichophyton and aspergillus. biosynthesis was measured both by incorporation of radiolabelled acetate and also by methylation of the sterol side chain. inhibition was accompanied by accumulation of radiolabel in 4,4-dimethylsterols and to a lesser extent in the 4-methylsterols, consistent with inhibition of lanosterol 14-demethylation. no other steps were affected. ergosterol biosynthes ... | 1990 | 2283585 |
| purification and molecular cloning of the "a" chain of a rat heteromeric ccaat-binding protein. sequence identity with the yeast hap3 transcription factor. | ccaat-binding factor (cbf) is a heteromeric mammalian transcription factor which binds to sequences containing a ccaat motif in a number of promoters such as those for type i collagen, albumin, mhc class ii, beta-actin, and others. it consists of two different components that are both needed for dna binding. we have purified the "a" chain of cbf to apparent homogeneity by sequence-specific dna affinity chromatography followed by mono s and mono q ion-exchange chromatography and obtained the amin ... | 1990 | 2266139 |
| spermidine biosynthesis in saccharomyces cerevisiae. biosynthesis and processing of a proenzyme form of s-adenosylmethionine decarboxylase. | we have cloned and sequenced the saccharomyces cerevisiae gene for s-adenosylmethionine decarboxylase. this enzyme contains covalently bound pyruvate which is essential for enzymatic activity. we have shown that this enzyme is synthesized as a mr 46,000 proenzyme which is then cleaved post-translationally to form two polypeptide chains: a beta subunit (mr 10,000) from the amino-terminal portion and an alpha subunit (mr 36,000) from the carboxyl-terminal portion. the protein was overexpressed in ... | 1990 | 2266128 |
| cell-cycle aspects of growth and maturation of mammalian oocytes. | in this review, recent data concerning growth and maturation of nonmammalian and mammalian female germ cells are compiled with regard to the increased understanding of somatic cells mitotic cycles, from yeast to human tissues. these data allow us to conclude that growing oocytes of nonvertebrates, lower vertebrates, and mammals resemble somatic cells in the g1 phase of the mitotic cycle in their metabolic and cell cycle behavior. transcriptional and translational activity of growing oocytes and ... | 1990 | 2264998 |
| inhibitory effect of transfer rna on protein kinases from baker's yeast and rat skeletal muscle. | sephadex g-200 gel filtration of dna cellulose-treated crude extracts of rat skeletal muscle, revealed a broad peak-fraction of trna-inhibitory protein kinases (pk) coeluted endogenous substrates. in comparison, the elution profile of baker's yeast exhibited multiple peak-fractions of trna-inhibiting pk. various trna all showed inhibition to pk. in the presence of regulatory subunit of cyclic amp-dependent protein kinase, trna did not exert synergetic inhibition on pk. moreover, the interaction ... | 1990 | 2257756 |
| sorting of peroxisomal membrane protein pmp47 from candida boidinii into peroxisomal membranes of saccharomyces cerevisiae. | a gene encoding pmp47, a peroxisomal integral membrane protein of the methylotrophic yeast candida boidinii, was isolated from a genomic library. dna sequencing of pmp47 revealed an open reading frame of 1269 base pairs capable of encoding a protein of 46,873 da. at least two membrane-spanning regions in the protein are predicted from the sequence. since the 3 amino acids at the carboxyl terminus are -ake, pmp47 lacks a typical peroxisomal sorting signal. no significant similarities in primary s ... | 1990 | 2243083 |
| the general mitochondrial matrix processing protease from rat liver: structural characterization of the catalytic subunit. | a critical step in the import of nuclear-encoded precursor proteins into mitochondria involves proteolytic cleavage of their amino-terminal leader peptides by processing proteases found in the mitochondrial matrix. we report here the characterization of the general matrix processing protease from rat liver mitochondria. the final enzyme preparation consisted of two polypeptides, a catalytically active 55-kda subunit and a 52-kda one. to deduce the complete primary structure of the 55-kda subunit ... | 1990 | 2236012 |
| an amino-terminal c-myc domain required for neoplastic transformation activates transcription. | the product of the c-myc proto-oncogene is a nuclear phosphoprotein whose normal cellular function has not yet been defined. c-myc has a number of biochemical properties, however, that suggest that it may function as a potential regulator of gene transcription. specifically, it is a nuclear dna-binding protein with a short half-life, a high proline content, segments that are rich in glutamine and acidic residues, and a carboxyl-terminal oligomerization domain containing the leucine zipper and he ... | 1990 | 2233723 |
| immunoreactivity of neoglycolipids constructed from oligomannosidic residues of the candida albicans cell wall. | to establish a model to study the immunoreactivity of oligosaccharidic structures from the candida albicans cell wall, we attempted to construct neoglycolipids with these residues by using oligomannosides released after mild acid hydrolysis of the phosphopeptidomannans isolated from yeast forms. from a mixture of manno-oligosaccharides ranging from mannobiose to mannononaose, the structure of a quantitatively major component (mannotriose) was determined to be man (beta 1-2) man (beta 1-2) man al ... | 1990 | 2228224 |
| purified presequence binding factor (pbf) forms an import-competent complex with a purified mitochondrial precursor protein. | in vitro mitochondrial import of the purified precursor form (potc) of rat ornithine carbamoyltransferase (otc) is stimulated by a cytosolic factor(s) contained in rabbit reticulocyte lysate. a protein factor that binds to potc but not to mature otc and was named presequence binding factor or pbf, was purified 91,000-fold from the lysate by affinity chromatography using potc-bound sepharose, deae-5pw hplc and sucrose gradient centrifugation. the purified pbf migrated as a single polypeptide of 5 ... | 1990 | 2209543 |
| ligand-specific transactivation of gene expression by a derivative of the human glucocorticoid receptor expressed in yeast. | in this study we have reconstituted transactivation of gene expression by the human glucocorticoid receptor in the yeast, saccharomyces cerevisiae. we have expressed the c-terminal half of the human glucocorticoid receptor (residues 415-777), the smallest derivative that can be expected to function as a ligand-dependent activator of transcription, in yeast cells. the function of the expressed protein has been assayed using a reporter gene consisting of the beta-galactosidase gene from escherichi ... | 1990 | 2203760 |
| in vivo expression of rat liver c-erba beta thyroid hormone receptor in yeast (saccharomyces cerevisiae). | to study thyroid hormone receptor (tr), we developed an in vivo expression system in yeast by using a copper-responsive yeast metallothionein promoter and ubiquitin-fusion protein technology. the cdna encoding full-length rat liver tr beta was expressed under the control of copper. the [125i]t3 binding activities to yeast extracts were significantly correlated with the added copper sulfate into the medium. partially purified tr from the transformed yeast had a high hormone binding affinity (kd = ... | 1990 | 2203342 |
| metabolic stability of 3-o-methyl-d-glucose in brain and other tissues. | 3-o-methyl-d-glucose (methylglucose) is often used to study blood-brain barrier transport and the distribution spaces of hexoses in brain. a critical requirement of this application is that it not be chemically converted in the tissues. recent reports of phosphorylation of methylglucose by yeast and heart hexokinase have raised questions about its metabolic stability in brain. therefore, we have re-examined this question by studying the metabolism of methylglucose by yeast hexokinase and rat bra ... | 1990 | 2200849 |
| elucidation of the deficiency in two yeast coenzyme q mutants. characterization of the structural gene encoding hexaprenyl pyrophosphate synthetase. | the assembly of a polyisoprenoid side chain and its transfer to para-hydroxybenzoate are the first two steps of coenzyme q biosynthesis. in yeast these reactions are catalyzed by hexaprenyl pyrophosphate synthetase and phb:polyprenyltransferase, respectively. we have screened nine complementation groups of yeast coenzyme q mutants for the activities of these two enzymes and found two strains deficient in either activity. the strain deficient in hexaprenyl pyrophosphate synthetase activity, c296- ... | 1990 | 2198286 |
| a candidate protein kinase c gene, pkc1, is required for the s. cerevisiae cell cycle. | probes derived from cdnas encoding isozymes of rat protein kinase c (pkc) were used to screen the genome of the budding yeast s. cerevisiae. a single gene (pkc1) was isolated that encodes a putative protein kinase closely related to the alpha, beta, and gamma subspecies of mammalian pkc. deletion of pkc1 resulted in recessive lethality. cells depleted of the pkc1 gene product displayed a uniform phenotype, a characteristic of cell division cycle (cdc) mutants, and arrested cell division at a poi ... | 1990 | 2196995 |
| cytoplasmic components of natural killer cells limit the growth of cryptococcus neoformans. | murine natural killer (nk) cell-mediated inhibition of growth of a yeast-like target cell, cryptococcus neoformans, was completely abrogated by blocking the effector cell secretory process with monensin. therefore, further studies were performed to determine the ability of various cytoplasmic fractions of nk cells to mediate inhibition of cryptococcal growth. percoll-fractionated homogenates of rat lgl tumor cells demonstrated that the granule-containing fractions plus three additional sets of l ... | 1990 | 2193078 |
| how different eukaryotic transcriptional activators can cooperate promiscuously. | a striking characteristic of many different eukaryotic transcriptional activators is their ability to activate gene expression synergistically. thus, for example, the rat glucocorticoid receptor and the yeast activator gal4 cooperatively activate transcription of a mammalian gene bearing binding sites for each of the proteins: activation by both activators is greater than the sum of the effects of each working alone. it would seem unlikely that these two proteins from such different organisms di ... | 1990 | 2188137 |
| 31p nmr measurements of the adp concentration in yeast cells genetically modified to express creatine kinase. | rabbit muscle creatine kinase has been introduced into the yeast saccharomyces cerevisiae by transforming cells with a multicopy plasmid containing the coding sequence for the enzyme under the control of the yeast phosphoglycerate kinase promoter. the transformed cells showed creatine kinase activities similar to those found in mammalian heart muscle. 31p nmr measurements of the near-equilibrium concentrations of phosphocreatine and cellular ph together with measurements of the total extractable ... | 1990 | 2185836 |
| rous sarcoma virus enhancer factor i is a ubiquitous ccaat transcription factor highly related to cbf and nf-y. | we have characterized enhancer factor i (efi), a trans-acting factor present in avian nuclear extracts which binds to the rous sarcoma virus long terminal repeat enhancer and promoter. through deletion and point mutagenesis, we show that efi is a member of the ccaat family of transcription factors. although the ccaat motif is essential for protein-dna recognition, efi shows surprising latitude in the nucleotide sequences flanking the ccaat motif to which it will bind with high affinity. efi will ... | 1990 | 2176209 |
| changing the invariant proline-30 of rat and drosophila melanogaster cytochromes c to alanine or valine destabilizes the heme crevice more than the overall conformation. | drosophila melanogaster and rat cytochromes c in which proline-30 was converted to alanine or valine were expressed in a strain of baker's yeast, saccharomyces cerevisiae, where they sustained aerobic growth. the mutations had no significant effect on the spectra or redox potentials but altered drastically the stability of the bond between the methionine-80 sulfur and the heme iron, as judged by four criteria: (i) the alkaline pka values of the 695-nm band of the ferric form of the mutant protei ... | 1990 | 2174161 |
| proton-linked sugar transport systems in bacteria. | the cell membranes of various bacteria contain proton-linked transport systems for d-xylose, l-arabinose, d-galactose, d-glucose, l-rhamnose, l-fucose, lactose, and melibiose. the melibiose transporter of e. coli is linked to both na+ and h+ translocation. the substrate and inhibitor specificities of the monosaccharide transporters are described. by locating, cloning, and sequencing the genes encoding the sugar/h+ transporters in e. coli, the primary sequences of the transport proteins have been ... | 1990 | 2172229 |
| protein kinase c in saccharomyces cerevisiae: comparison with the mammalian enzyme. | protein kinase c (pkc) was detected in the yeast saccharomyces cerevisiae with bovine myelin basic protein as the phosphate acceptor. the enzyme was purified at least 500-fold by a four-step column chromatographic procedure (phenyl-sepharose cl-4b, mono q, heparin-5pw, and hydroxyapatite). the molecular mass was approximately 90 kda, as estimated by gel-filtration analysis. yeast pkc was activated by the simultaneous addition of ca2+, diacylglycerol, and phosphatidylserine. free arachidonic acid ... | 1990 | 2164217 |
| tissue and species distribution of liver type and tumor type nuclear poly(a) polymerases. | previous studies in this laboratory have identified two distinct nuclear poly(a) polymerases, a 48 kda tumor type enzyme and a 36-38 kda liver type enzyme. to investigate the tissue and species specificity of these enzymes, nuclear extracts were prepared from various rat tissues, pig brain and two human cell lines. these as well as whole cell extract from yeast were probed for the two enzymes by immunoblot analysis using polyclonal anti-tumor poly(a) polymerase antibodies or autoimmune sera whic ... | 1990 | 2162661 |
| molecular cloning of mevalonate kinase and regulation of its mrna levels in rat liver. | mevalonate kinase [atp:(r)-mevalonate 5-phosphotransferase, ec 2.7.1.36] may be a regulatory site in the cholesterol biosynthetic pathway, and a mutation in the gene coding for this enzyme is thought to cause the genetic disease mevalonic aciduria. to characterize this enzyme, a rat liver cdna library was screened with a monospecific antibody, and a 1.7-kilobase cdna clone coding for mevalonate kinase was isolated. the complete dna sequence was determined, and the longest open reading frame code ... | 1990 | 2158094 |
| characteristics of l-alanine:4,5-dioxovaleric acid transaminase: an alternate pathway of heme biosynthesis in yeast. | the present study reports for the first time the presence of the enzyme l-alanine:4,5-dioxovaleric acid transaminase (ec 2.6.1.43), which catalyzes the irreversible synthesis of 5-aminolevulinic acid in three strains of yeast: candida albicans 3100, saccharomyces cerevisiae 3059, and s. cerevisiae s288c. the enzyme was predominantly present in the cytosol and was purified from c. albicans 3100 by about 200-fold with an overall yield of 23% to apparent homogeneity. the purification procedure invo ... | 1990 | 2154954 |
| rat homologs of the drosophila dunce gene code for cyclic amp phosphodiesterases sensitive to rolipram and ro 20-1724. | the dunce locus of drosophila melanogaster codes for a low km, camp phosphodiesterase. the correct function of this gene is required for normal learning and memory activity in flies, because dunce mutants fail in tests of behavioral conditioning. these observations have indicated that camp regulation is an important aspect of the biochemistry underlying learning and memory processes in insects. to determine whether the locus is functionally conserved in mammals, we have expressed dunce gene homo ... | 1990 | 2153912 |
| inactivity of terbinafine in a rat model of pulmonary aspergillosis. | in a model of bronchopulmonary aspergillosis terbinafine did not improve survival of experimental animals in doses up to 80 mg/kg/day despite adequate lung concentrations. pretreatment and aerosolization of the compound were also ineffective. terbinafine was markedly less active in vitro when serum was used instead of yeast-nitrogen-glucose-broth. it is concluded that a lack of bioavailability in the presence of serum may explain the lack of activity of terbinafine in experimental aspergillosis. | 1990 | 2128278 |
| identification of a putative yeast homolog of the mammalian beta chains of the clathrin-associated protein complexes. | the clathrin-associated protein complexes are heterotetrameric structures believed to interact with clathrin and with membrane components of mammalian coated pits and coated vesicles. i have identified a yeast homolog of the mammalian beta-type large chains, suggesting the existence in yeast cells of clathrin-associated protein complexes. a sequence comparison between the putative yeast beta-type chain and its mammalian counterparts shows that their amino-terminal domains are related over their ... | 1990 | 2122239 |
| cloning and expression of rat histidase. homology to two bacterial histidases and four phenylalanine ammonia-lyases. | histidase (histidine ammonia-lyase, ec 4.3.1.3) catalyzes the deamination of histidine to urocanic acid. apart from phenylalanine ammonia-lyase, which is not expressed in animals, histidase is the only enzyme known to have a dehydroalanine residue in its active site. the amino site precursor and the mechanism of formation of dehydroalanine are not known. as an initial step to determining the precursor of dehydroalanine in histidase, we have isolated a functional cdna clone for histidase from a r ... | 1990 | 2120224 |
| effects of expression of mammalian g alpha and hybrid mammalian-yeast g alpha proteins on the yeast pheromone response signal transduction pathway. | scg1, the product of the saccharomyces cerevisiae scg1 (also called gpa1) gene, is homologous to the alpha subunits of g proteins involved in signal transduction in mammalian cells. scg1 negatively controls the pheromone response pathway in haploid cells. either pheromonal activation or an scg1 null mutation relieves the negative control and leads to an arrest of cell growth in the g1 phase of the cell cycle. expression of rat g alpha s was previously shown to complement the growth defect of scg ... | 1990 | 2111439 |
| simultaneous determination of pantothenic acid and hopantenic acid in biological samples and natural products by gas chromatography-mass fragmentography. | a method for the simultaneous determination of pantothenic acid and hopantenic acid in plasma samples was developed using gas chromatography-mass spectrometry with multiple ion detection. plasma samples were directly purified without deproteinization on an ion-exchange resin, and the eluate was extracted with ethyl acetate under acidic conditions. the organic layer was evaporated to dryness under a stream of nitrogen, and the residue was dissolved in an internal standard solution. pantothenic an ... | 1990 | 2109760 |
| genotoxic risk associated with pesticides: evidences on short-term tests. | 1990 | 2097665 | |
| [analgesic and antipyretic effects of cyproheptadine]. | cyproheptadine-hcl raised the pain thresholds during hot plat test and writhing test in mice and tail flick test in rats, strengthened the hypnotic action by subthreshold dosage of sodium pentobarbital and chloral hydrate. the ed50 were 4.4 (3.2-5.7) and 12.4 (8.4-18.2) mg/kg 30 min after ip cyproheptadine in mice and rats, respectively. the ed50 was 0.14 (0.12-0.18) mg/kg 90 min after icv cyproheptadine in mice. cyproheptadine po 20, 40 mg/kg and ip 10, 20 mg/kg showed significant antipyretic e ... | 1990 | 2087993 |
| [low-molecular peptides in food as factors altering the resistance of rats to emotional stress]. | the authors studied the resistance of animals to emotional stress when they were given in the diet, as a source of protein, casein and a protein fraction composed of a mixture of amino acids and low-molecular peptides (18 and 2.7%) obtained by reprocessing saccharomyces cerevisiae yeasts, biomass. the ecg, rheovasogram, systolic arterial pressure, and respiratory rate were studied in rats given a diet containing 18 and 2.7% low-molecular peptides (controls) and in the same animals after 24-hour ... | 1990 | 2080075 |
| influence of the presence of a methyl group on the myocardial metabolism of 15-(paraiodophenyl)-3 methyl pentadecanoic acid (imppa). | the objective of the present study was to determine the mechanism of accumulation of myocardial activity following i.v. injection of 15-(paraiodophenyl)-3 methyl pentadecanoic acid (imppa). imppa and 15 phenyl-3 methyl pentadecanoic acid (mppa) were labeled with 14c at position 1 and used to perfuse isolated rat hearts in a closed system. after 5 min of perfusion, imppa reached 2/3 of its value at 45 min. 14co2 production was low. most of the myocardial activity was in the form of free imppa. an ... | 1990 | 2079420 |
| molecular cloning of aldolases for synthetic applications. | 1990 | 2076016 | |
| effect of chronic ethanol consumption on selenium status and utilization in rats. | effects of chronic ingestion of 2 levels of alcohol on selenium (se) utilization were determined in initially se-depleted rats. male weanling rats were fed ad lib a se deficient (0.012 mg/kg) basal diet for 4 weeks and then were meal-fed low or marginally adequate se in the form of high se yeast for 4 weeks. during se repletion, ethanol, which replaced medium-chain triglycerides in the diet, provided 10 or 20 percent of food energy. the basal diet provided 80% of food energy as well as adequate ... | 1991 | 2064758 |
| influence of antibiotics on the recovery and kinetics of saccharomyces boulardii in rats. | saccharomyces boulardii (sb) is a yeast that is used for the prevention and treatment of antibiotic-associated diarrhea and for the treatment of pseudomembranous colitis. since sb will most commonly be used when the bacterial flora of the gastrointestinal tract have been disrupted by antibiotic treatment, the influence of different antibiotics on the kinetics and recovery of sb in feces was investigated in rats. following a single oral dose, sb concentrations in feces were measured for periods o ... | 1991 | 2062812 |
| nucleotide sequence of the yeast glutathione s-transferase cdna. | the nucleotide sequence (658 bp) of the cdna coding for glutathione s-transferase y-2 of yeast issatchenkia orientalis was obtained. the cdna clone contains an open reading frame of 570 nucleotides encoding a polypeptide comprising 190 amino acids with a molecular weight of 21,520. the primary amino acid sequence of the enzyme exhibits only 25.0% and 21.1% identity with 177 and 151 amino acid residues of maize glutathione s-transferase i and rat glutathione s-transferase yb2, respectively. | 1991 | 2054388 |
| a fraction derived from brewer's yeast inhibits cholesterol synthesis by rat liver preparations in vitro. | brewer's yeast was grown on a defined medium containing tracer 51cr with or without added chromium. the two batches of yeast contained 10 microgram/g (high-cr) or 80 ng/g (low-cr). extracts were prepared and fractionated. a third batch of yeast (third batch) was grown with added cr, and fractionated. rats were reared on either rat cubes (normal diet) or on a low-cr diet (low-cr), or on rat cubes with added cholestyramine (cholestyramine diet). preparations of rat liver, both cell-free and intact ... | 1991 | 2043605 |
| purification and characterization of recombinant tissue kallikrein from escherichia coli and yeast. | a full-length rat tissue kallikrein cdna was constructed by oligonucleotide engineering through an extension of rsk 1105, a partial cdna clone containing 534 bp of the 3' end of tissue kallikrein, followed by site-directed mutagenesis to remove the vector sequence from within the chimaeric coding sequence. the cdna has been cloned both into the plasmid pet3b under the control of the t7 promoter/polymerase system, and into the shuttle vector pye directed by the alpha-factor promoter. expression i ... | 1991 | 2039483 |
| evidence that rat liver pyruvate dehydrogenase kinase activator protein is a pyruvate dehydrogenase kinase. | it is shown here that rat liver pyruvate dehydrogenase (pdh) kinase activator protein (kap) catalyses atp-dependent inactivation and [32p]phosphorylation of pig heart pdhe1 and of yeast (saccharomyces cerevisiae) pdh complex devoid of pdh kinase activity, that fluorosulphonylbenzoyladenosine inactivates rat liver kap and the intrinsic pdh kinase of rat liver pdh complex, and that kap, like pdh kinase, is inactivated by thiol-reactive reagents. it is concluded that kap is a free pdh kinase. | 1991 | 2039454 |
| transcription initiated by rna polymerase ii and transcription factors from liver. structure and action of transcription factors epsilon and tau. | we have fractionated rat liver and identified a set of transcription factors that are essential for accurate initiation by rna polymerase ii. these factors were resolved into five distinct enzyme fractions designated alpha, beta gamma, delta, epsilon, and tau. four of these fractions can now be replaced with purified proteins. alpha and beta gamma were previously purified to apparent homogeneity (conaway, j. w., and conaway, r. c. (1989) j. biol. chem. 264, 2357-2362). here, we report purificati ... | 1991 | 2019603 |
| analysis of rat natural killer cytotoxic factor (nkcf): mechanism of action and relationship to other cytotoxic/cytostatic factors. | natural killer cytotoxic factor (nkcf) has been proposed as one of the factors that mediates lysis induced by natural killer (nk) cells. recently, an excellent source of nkcf has been found to be the rat large granular lymphocyte (lgl) tumor (rnk) cell line. in this study, the kinetics of lysis of the nk-sensitive, tumor target yac-1 by the rnk-nkcf was analyzed and found to parallel that seen with nk cell-mediated killing. rnk-nkcf was also capable of killing the nk-resistant target cell, mbl-2 ... | 1991 | 2018982 |
| stearoyl-acyl-carrier-protein desaturase from higher plants is structurally unrelated to the animal and fungal homologs. | stearoyl-acyl-carrier-protein (acp) desaturase (ec 1.14.99.6) was purified to homogeneity from avocado mesocarp, and monospecific polyclonal antibodies directed against the protein were used to isolate full-length cdna clones from ricinus communis (castor) seed and cucumis sativus (cucumber). the nucleotide sequence of the castor clone prcd1 revealed an open reading frame of 1.2 kilobases encoding a 396-amino acid protein of 45 kda. the cucumber clone pcsd1 encoded a homologous 396-amino acid pr ... | 1991 | 2006187 |
| an "attenuator domain" is sandwiched by two distinct transactivation domains in the transcription factor c/ebp. | c/ebp is a rat liver dna-binding protein which can act as a transcription factor. its n-terminal portion contains three distinct domains. the first domain (amino acids 1 to 107) appears to be a highly potent transactivator. the second domain (amino acids 107 to 170) does not appear to exhibit either activation or repression activity. this domain is defined as an "attenuator domain" because its presence under four different sequence contexts reproducibly decreases the effect of transactivation of ... | 1991 | 1996105 |
| no detection of characteristic fungal protein elongation factor ef-3 in pneumocystis carinii. | the taxonomic status of pneumocystis carinii is uncertain, and p. carinii has been categorized both as a fungus and as a protozoan. recent comparisons of rna sequence homologies between p. carinii and several genera of fungi and protozoa suggest that p. carinii has closer affinities with the ascomycetes than with the protozoa. the translatory systems of the fungi, however, require three soluble protein factors for peptide chain elongation rather than the two necessary in other eukaryotic systems ... | 1991 | 1995744 |
| intracellular leucine zipper interactions suggest c-myc hetero-oligomerization. | the physiological significance of in vitro leucine zipper interactions was studied by the use of two strategies which detect specific protein-protein interactions in mammalian cells. fusion genes were constructed which produce chimeric proteins containing leucine zipper domains from several proteins fused either to the dna-binding domain of the saccharomyces cerevisiae gal4 protein or to the transcriptional activation domain of the herpes simplex virus vp16 protein. previous studies in mammalian ... | 1991 | 1990293 |
| covalent structure, disulfide bonding, and identification of reactive surface and active site residues of human prostatic acid phosphatase. | the pairing of the half-cysteine residues of human prostatic acid phosphatase was established by proteolytic digestion and analysis of the resulting peptide mixtures by fast atom bombardment mass spectrometry (fab-ms). an independently derived, full length cdna clone was used as the basis for the interpretation of the fab-ms data. the sequence of the native protein is that predicted from the present cdna sequence, except for the carboxyl-terminal end and some possible post-translational deamidat ... | 1991 | 1989985 |
| rat liver polysome n alpha-acetyltransferase: isolation and characterization. | rat liver polysome n alpha-acetyltransferase has been purified to homogeneity by a four-step procedure that utilizes ammonium sulfate precipitation, gel filtration, hydroxylapatite chromatography, and mono q ion exchange chromatography. the enzyme is greatly stabilized by the inclusion of edta and 0.01% deoxycholate in the isolation buffers. the purified enzyme has a native molecular weight of 190,000 and a subunit molecular weight of 95,000, suggesting that it is a homodimer. the enzyme shows a ... | 1991 | 1989673 |
| identification of a cdna encoding a second putative prohormone convertase related to pc2 in att20 cells and islets of langerhans. | pc2 and furin are two recently identified members of a class of mammalian proteins homologous to the yeast precursor processing protease kex2 and the bacterial subtillisins. we have used the polymerase chain reaction to identify and clone a cdna (pc3) from the mouse att20 anterior pituitary cell line that represents an additional member of this growing family of mammalian proteases. pc3 encodes a 753-residue protein that begins with a signal peptide and contains a 292-residue domain closely rela ... | 1991 | 1988934 |
| thalidomide induced alteration in secondary structure of rat embryonic dna in vivo. | teratogenicity of thalidomide was demonstrated in wistar rats following a single maternal intravenous injection during the embryonic organogenetic period. when compared to day 13 embryonic dna isolated from untreated control mothers, differences were observed in the mean wet weights of day 13 embryos from rats treated with thalidomide during days 10 or 11 of gestation, and significantly less amounts of embryonic dna were recovered from mothers similarly treated on days 10 or 12 of their respecti ... | 1990 | 1980031 |
| the ole1 gene of saccharomyces cerevisiae encodes the delta 9 fatty acid desaturase and can be functionally replaced by the rat stearoyl-coa desaturase gene. | strains of saccharomyces cerevisiae bearing the ole1 mutation are defective in unsaturated fatty acid (ufa) synthesis and require ufas for growth. a previously isolated yeast genomic fragment complementing the ole1 mutation has been sequenced and determined to encode the delta 9 fatty acid desaturase enzyme by comparison of primary amino acid sequence to the rat liver stearoyl-coa desaturase. the ole1 structural gene encodes a protein of 510 amino acids (251 hydrophobic) having an approximate mo ... | 1990 | 1978720 |
| regulation of the pituitary-specific homeobox gene ghf1 by cell-autonomous and environmental cues. | homeodomain proteins function in determination of mating type in yeast, segmentation in fruit flies and cell-type specific gene expression in mammals. in drosophila, expression of homeobox genes is controlled by cell-autonomous interactions between regulatory proteins and environmental clues. similar controls may operate during mammalian limb development and frog embryogenesis. but, the exact way in which expression of homeodomain proteins is regulated in these systems is not clear and requires ... | 1990 | 1972784 |
| yeast acetyl-coa carboxylase: in vitro phosphorylation by mammalian and yeast protein kinases. | acetyl-coa carboxylase (acc) is regulated in mammalian tissues, in part, by multisite enzyme phosphorylation. yeast acc (y-acc) has been highly purified from s. cerevisiae by monomeric avidin-sepharose chromatography, revealing an enzyme subunit species of molecular mass 265,000 da. unlike mammalian enzyme, y-acc is citrate-independent, and reacts weakly or not at all with a panel of anti-rat liver acc antibodies. like rat acc, y-acc is rapidly phosphorylated and inactivated by two mammalian car ... | 1990 | 1972618 |
| homologous sugar transport proteins in escherichia coli and their relatives in both prokaryotes and eukaryotes. | separate proteins for proton-linked transport of d-xylose, l-arabinose, d-galactose, l-rhamnose and l-fucose into escherichia coli are being studied. by cloning and sequencing the appropriate genes, the amino acid sequences of proteins for d-xylose/h+ symport (xyle), l-arabinose/h+ symport (arae), and part of the protein for d-galactose/h+ symport (galp) have been determined. these are homologous, with at least 28% identical amino acid residues conserved in the aligned sequences, although their ... | 1990 | 1970645 |
| rhizobium meliloti adenylate cyclase is related to eucaryotic adenylate and guanylate cyclases. | a gene from rhizobium meliloti coding for an adenylate cyclase was sequenced, and the deduced protein sequence was compared with those of other known adenylate cyclases. no similarity could be detected with the procaryotic counterparts. however, striking similarity was found with the catalytic region of saccharomyces cerevisiae adenylate cyclase, the cytoplasmic domains of bovine adenylate cyclase, and two mammalian guanylate cyclases. the gene was fused to the enteric beta-galactosidase, and th ... | 1990 | 1970565 |
| phosphoglucoisomerase-catalyzed interconversion of hexose phosphates. a model for d-[2-3h]glucose metabolism in human erythrocytes. | when d-[2-3h]glucose 6-phosphate mixed with the unlabeled ester is converted to d-[1-3h]fructose 6-phosphate and 3hoh in the phosphoglucoisomerase reaction and then to d-[1-3h]fructose 1,6-bisphosphate in the phosphofructokinase reaction, the specific radioactivity of the latter metabolite and the production of 3hoh relative to the total generation of tritiated end products are both inversely related to the concentration of phosphofructokinase. in human erythrocytes, the modeling of d-[2-3h]gluc ... | 1991 | 1931158 |
| cloning and characterization of a 3-methyladenine dna glycosylase cdna from human cells whose gene maps to chromosome 16. | we described previously the isolation of a saccharomyces cerevisiae 3-methyladenine (3-meade) dna glycosylase repair gene (mag) by its expression in glycosylase-deficient escherichia coli alka tag mutant cells and its ability to rescue these cells from the toxic effects of alkylating agents. here we extend this cross-species functional complementation approach to the isolation of a full-length human 3-meade dna glycosylase cdna that rescues alka tag e. coli from killing by methyl methanesulfonat ... | 1991 | 1924375 |
| a rat brain mrna encoding a transcriptional activator homologous to the dna binding domain of retroviral integrases. | we have isolated a rat cdna, named fe65, hybridizing to an mrna of about 2,300 nucleotides present in rat brain, undetectable in rat liver and very poorly represented in other tissues. an mrna of the same size is present in human neuroblastoma cells and is absent from other human cell lines. the fe65 cdna contains an open reading frame (orf) coding for a polypeptide of 499 amino acids in which 143 residues can be aligned with the dna binding domain of the integrases encoded by mammalian immunode ... | 1991 | 1923810 |
| dominant inhibitory mutations in the mg(2+)-binding site of rash prevent its activation by gtp. | we have previously demonstrated that substitution of asn for ser at position 17 of rash yields a dominant inhibitory protein whose expression in cells interferes with endogenous ras function (l. a. feig, and g. m. cooper, mol. cell. biol. 8:3235-3243, 1988). subsequent structural studies have shown that the hydroxyl group of ser-17 contributes to the binding of mg2+ associated with bound nucleotide. in this report, we show that more subtle amino acid substitutions at this site that would be expe ... | 1991 | 1922022 |
| structural homology among mammalian and saccharomyces cerevisiae isoprenyl-protein transferases. | farnesyl-protein transferase (ftase) purified from rat or bovine brain is an alpha/beta heterodimer, comprised of subunits having relative molecular masses of approximately 47 (alpha) and 45 kda (beta). in the yeast saccharomyces cerevisiae, two unlinked genes, ram1/dpr1 (ram1) and ram2, are required for ftase activity. to explore the relationship between the mammalian and yeast enzymes, we initiated cloning and immunological analyses. cdna clones encoding the 329-amino acid cooh-terminal domain ... | 1991 | 1918005 |
| effects of protein restriction on functional properties of rat peritoneal macrophages. | rats were maintained on 20% and 4% protein diets for 3 weeks. the functional properties of thioglycollate (tg) elicited macrophages from these groups were compared with the non elicited resident cells from the protein fed group. elicitation of macrophages in response to tg was low in the protein deficient group. these cells also exhibited low adherence in overnight cultures compared to those isolated from the protein fed group; however their viability and total protein content remained unaltered ... | 1991 | 1916946 |
| interferon-gamma activates rat alveolar macrophages for anticryptococcal activity. | cryptococcus neoformans is a pathogenic yeast causing disease predominantly in immunosuppressed patients. c. neoformans is acquired by the pulmonary route, where the alveolar macrophage (am) is a resident mechanism of host defense. the ability of rat am to be activated by products of the immune response for enhanced anticryptococcal effect has not previously been demonstrated. rat am could be activated in vitro for anticryptococcal activity by medium conditioned by concanavalin a-stimulated sple ... | 1991 | 1908686 |
| assessment of the transcriptional activation potential of the hmg chromosomal proteins. | chromosomal proteins hmg-14, hmg-17, and hmg-1 are among the most abundant, ubiquitous, and evolutionarily conserved nonhistone proteins. analysis of their structure reveals features which are similar to those of certain transcription factors. the distribution of charged amino acid residues along the polypeptide chains is asymmetric: positive charges are clustered toward the n-terminal region, while negative charges are clustered toward the c-terminal region. the residues in the c-terminal regio ... | 1991 | 1908554 |
| two glycogen synthase isoforms in saccharomyces cerevisiae are coded by distinct genes that are differentially controlled. | in previous work, we identified a saccharomyces cerevisiae glycogen synthase gene, gsy1, which codes for an 85-kda polypeptide present in purified yeast glycogen synthase (farkas, i., hardy, t.a., depaoli-roach, a.a., and roach, p.j. (1990) j. biol. chem. 265, 20879-20886). we have now cloned another gene, gsy2, which encodes a second s. cerevisiae glycogen synthase. the gsy2 sequence predicts a protein of 704 residues, molecular weight 79,963, with 80% identity to the protein encoded by gsy1. a ... | 1991 | 1908457 |
| in vitro identification of a soluble protein:geranylgeranyl transferase from rat tissues. | the gamma subunit of mammalian trimeric g proteins has been shown previously to be modified in vivo on a cysteine residue situated at the carboxyl-terminal sequence-cys-ala-ile-leu-cooh by a 20-carbon prenyl moiety geranylgeranyl (mumby, s. m., casey, p. j., gilman, a. g., gutowski, s., and sternweis, p. c. (1990) proc. natl. acad. sci. u.s.a. 87, 5873-5877; yamane, h. k., farnsworth, c. c., xie, h., howald, w., fung, b. k-k., clarke, s., gelb, m. h., and glomset, j. a. (1990) proc. natl. acad. ... | 1991 | 1906876 |
| analyzing the substrate specificity of saccharomyces cerevisiae myristoyl-coa:protein n-myristoyltransferase by co-expressing it with mammalian g protein alpha subunits in escherichia coli. | a dual plasmid system was used to examine the protein and acyl-coa specificities of saccharomyces cerevisiae myristoyl-coa:protein n-myristoyltransferase (nmt) by co-expressing it in escherichia coli with each of four homologous alpha subunits of the signal-transducing, heterotrimeric g proteins. exogenous [3h]myristate was incorporated into rat gi alpha 1 and rat go alpha but not into bovine gs alpha or human gz alpha. oxygen for methylene group substitutions in myristate result in analogs with ... | 1991 | 1903791 |
| fluorescent labeling of the nucleotide site in cytosolic rat liver phosphoenolpyruvate carboxykinase. | reaction of rat liver phosphoenolpyruvate carboxykinase (gtp: oxaloacetate carboxy-lyase (transphosphorylating), ec 4.1.1.32) with the alkylating fluorescent probe n-(iodoacetylaminoethyl)-5-naphthylamine-1-sulfonic acid (1,5-i-aedans), results in complete loss of enzymatic activity. one mole of the fluorescent reagent is incorporated per mole of the inactivated enzyme. when the modification is carried out in the presence of gdpmn, the enzyme retains 97% of its activity with almost no incorporat ... | 1991 | 1897968 |
| comparative study of three recombinant hirudins with heparin in an experimental venous thrombosis model. | three recombinant hirudins (r-hirudins) produced by genetic processes from escherichia coli and yeast were studied. r-hirudins could be an alternative treatment to heparin; so, the antithrombotic activity of these drugs should be compared to heparin, the reference substance, in an experimental venous thrombosis model. in this model, the effect of these r-hirudins on thrombus weight reduction were not identical. they varied depending on the original product (e. coli or yeast). the growth-inhibiti ... | 1991 | 1894201 |
| competitive inhibition of liver glucokinase by its regulatory protein. | the regulatory protein of rat liver glucokinase (hexokinase iv or d) behaved as a fully competitive inhibitor of this enzyme when glucose was the variable substrate, i.e. it increased the half-saturating concentration of glucose as a linear function of its concentration without affecting v (velocity at infinite concentration of substrate). the inhibition by the regulatory protein and that by palmitoyl-coa were synergistic with that by n-acetyl-glucosamine, indicating that the two former inhibito ... | 1991 | 1889417 |
| different substrate specificities of lanosterol 14a-demethylase (p-45014dm) of saccharomyces cerevisiae and rat liver for 24-methylene-24,25-dihydrolanosterol and 24,25-dihydrolanosterol. | the purified lanosterol 14a-demethylase (p-45014dm) of s. cerevisiae catalyzed the 14a-demethylation of 24-methylene-24,25-dihydrolanosterol (24-methylenelanost-8-en-3 beta-ol, 24-methylene-dhl), the natural substrate of the demethylase of filamentous fungi, as well as its natural substrate, lanosterol. lanosterol 14a-demethylase of rat liver microsomes also catalyzed the 14a-demethylation of 24-methylene-dhl, but the activity was considerably lower than that for lanosterol. the activity of the ... | 1991 | 1872829 |
| a common structural motif in nuclear pore proteins (nucleoporins) | 1991 | 1872825 | |
| radiochemical assay of adenylosuccinase: demonstration of parallel loss of activity toward both adenylosuccinate and succinylaminoimidazole carboxamide ribotide in liver of patients with the enzyme defect. | a radiochemical assay for adenylosuccinase, an enzyme which intervenes twice in the biosynthesis of adenine nucleotides, has been developed. the two substrates of the enzyme, succinylaminoimidazole carboxamide ribotide (saicar) and adenylosuccinate (s-amp), were synthesized in radioactive form by incubating [2,3-14c]fumarate and, respectively, aicar and amp with partially purified adenylosuccinase from yeast. enzyme activities were determined by measuring the release of labeled fumarate after it ... | 1991 | 1872474 |
| inhibition of long-chain acyl-coa synthetase by the peroxisome proliferator perfluorodecanoic acid in rat hepatocytes. | perfluorodecanoic acid (pfda) is a potent peroxisome proliferator and is known to affect hepatic lipid metabolism in rats. the effects of pfda on fatty acid utilization were examined in isolated rat hepatocyte suspensions and in rat liver mitochondria and microsomes. pfda inhibited the oxidation of palmitic acid but not octanoic or pyruvic acids when hepatocytes were incubated with 1 mm pfda. at this pfda concentration the esterification of palmitic acid into triacylglycerols was also reduced. t ... | 1991 | 1859447 |
| chromatography of selenoproteins in human serum using matrix-bound heparin. | since previous experiments indicated that a major selenoprotein in human serum interacts with heparin, chromatography of serum on matrix-bound heparin was studied. when human serum was applied to heparin-agarose columns, approximately half of the applied selenium was not retained on the columns. approx. 40% of the selenium could then be eluted either with increasing concentrations of heparin or ammonium acetate. using a scaled-down version of this procedure, selenoproteins from 0.5 ml serum were ... | 1991 | 1856049 |
| [response of the ird intestinal epithelial cell line to clostridium difficile toxins a and b in rats. effect of saccharomyces boulardii]. | in vivo, clostridium difficile acts by releasing 2 toxins: toxin a, an enterotoxin, and toxin b, a cytotoxin. this study was performed to determine: a) whether the rat epithelial intestinal cell line ird 98 responds to clostridium difficile toxin a and b; b) whether the yeast saccharomyces boulardii has an effect on this model. evaluation of 3h-thymidine incorporation into ird 98 cells exposed to toxin b revealed that dna synthesis was inhibited for low concentrations (10 ng/ml). for higher conc ... | 1991 | 1849105 |
| zinc fingers, zinc clusters, and zinc twists in dna-binding protein domains. | we now recognize three distinct motifs of dna-binding zinc proteins: (i) zinc fingers, (ii) zinc clusters, and (iii) zinc twists. until very recently, x-ray crystallographic or nmr three-dimensional structure analyses of dna-binding zinc proteins have not been available to serve as standards of reference for the zinc binding sites of these families of proteins. those of the dna-binding domains of the fungal transcription factor gal4 and the rat glucocorticoid receptor are the first to have been ... | 1991 | 1846973 |
| a small gtp-binding protein dissociates from synaptic vesicles during exocytosis. | low-molecular-weight gtp-binding proteins are strong candidates for regulators of membrane traffic. in yeast, mutations in the sec4 or ypt1 genes encoding small gtp-binding proteins inhibit constitutive membrane flow at the plasma membrane or golgi complex, respectively. it has been suggested that membrane fusion-fission events are regulated by cycling of small gtp-binding proteins between a membrane-bound and free state, but although most of these small proteins are found in both soluble and ti ... | 1991 | 1845915 |
| screening a yeast promoter library leads to the isolation of the rp29/l32 and snr17b/rpl37a divergent promoters and the discovery of a gene encoding ribosomal protein l37. | two promoters (a7 and a23), isolated at random from the saccharomyces cerevisiae genome by virtue of their capacity to activate transcription, are identical to known intergenic bidirectional promoters. sequence analysis of the genomic dna adjacent to the a7 promoter identified a split gene encoding ribosomal (r) protein l37, which is homologous to the trna-binding r-proteins, l35a (from human and rat) and l32 (from frogs). | 1991 | 1840541 |
| the primary structure of rat ribosomal protein l23. | the amino acid sequence of the rat 60s ribosomal subunit protein l23 was deduced from the sequence of nucleotides in two recombinant cdnas. ribosomal protein l23 has 140 amino acids and a molecular weight of 14,856. hybridization of the cdna to digests of nuclear dna suggests that there are 7-9 copies of the l23 gene. the mrna for the protein is about 600 nucleotides in length. rat l23 is homologous to saccharomyces cerevisiae l17a and related to escherichia coli l14 and other members of the pro ... | 1991 | 1840488 |
| multiple forms of dynamin are encoded by shibire, a drosophila gene involved in endocytosis. | dynamin was discovered in bovine brain tissue as a nucleotide-sensitive microtubule-binding protein of relative molecular mass 100,000. it was found to cross-link microtubules into highly ordered bundles, and appeared to have a role in intermicrotubule sliding in vitro. cloning and sequencing of rat brain dynamin complementary dna identified an n-terminal region of about 300 amino acids which contained the three consensus elements characteristic of gtp-binding proteins. extensive homology was fo ... | 1991 | 1828536 |
| topography of very-long-chain-fatty-acid-activating activity in peroxisomes from rat liver. | we have investigated the localization of palmitoyl-coa (hexadecanoyl-coa) synthetase (ec 6.2.1.3) and cerotoyl-coa (hexacosanoyl-coa) synthetase in peroxisomes isolated from rat liver. palmitoyl-coa and cerotoyl-coa synthetases, like acyl-coa: dihydroxyacetone phosphate acyltransferase (ec 2.3.1.42), are present in the peroxisomal membrane. trypsin treatment of intact peroxisomes led to the disappearance of both palmitoyl-coa and cerotoyl-coa synthetase activities but had little, if any, effect ... | 1991 | 1828148 |
| characterization of growth hormone releasing factor analog expression in saccharomyces cerevisiae. | an analog of growth hormone releasing factor (grf), [leu27]grf(1-40)-oh, has been expressed and secreted in saccharomyces cerevisiae under the control of the alpha-factor gene promoter and prepro sequence. a single pair of consecutive basic residues served as a processing site between the alpha-factor sequences and the grf sequences. [leu27]grf(1-40)-oh from fermentor broth containing 20-30 mg/l of immunoreactive peptides was shown to be correctly processed and to possess biological activity as ... | 1991 | 1802859 |
| [characteristics of structural changes in the internal organs of animals after administration of dried yeast]. | morphofunctional analysis of the state of internal organs was made in rats which received varying amounts of mushroom powder. it has been established that the new product induces development of endovasculitis, dystrophy and lympho-histiocytic infiltrates of parenchymatous organs. the data obtained have evidenced the development of delayed allergic reactions to mushroom powder. a conclusion has been made on the necessity of its limited inclusion into food ration. | 1991 | 1796575 |
| prohormone-converting enzymes: regulation and evaluation of function using antisense rna. | several putative peptide-processing endoproteases have been identified by homology to the yeast kex2 endoprotease, including furin, pc2, and pc1. however, the question is still open as to which might be involved in peptide posttranslational processing. to enable detailed comparisons of physiological changes in peptide processing with biochemical and molecular biological studies, we cloned rat pituitary cdnas for pc1 and pc2. the amino acid sequence homologies among rat, human, and mouse pc1, pc2 ... | 1991 | 1791845 |
| [a preliminary study on the anti-mutagenic effect of seleno-malt]. | seleno-malt is an organic selenium product recently developed in china. seleno-malt itself is not genotoxic. it antagonized the mutagenic effect of aflatoxin b1. its antagonistic activity was 10-15 times higher then seleno-yeast. seleno-malt may be used as a food additive in areas with low selenium level for the prevention of ke-shan disease and cancer. | 1991 | 1786748 |
| primary structure and tissue distribution of anglerfish carboxypeptidase h. | most peptide hormones are synthesized as part of larger precursor proteins which must be processed after translation to generate bioactive peptides. this usually involves cleavage of the precursor by an endopeptidase at sites marked by basic amino acids, followed by removal of n- or c-terminal basic residues by the action of an aminopeptidase or carboxypeptidase. these processing events have been observed in a variety of species, from yeast to mammals. as part of an effort to characterize prohor ... | 1991 | 1778303 |
| nucleotide sequence of a human heart cdna encoding the mitochondrial phosphate carrier. | we have isolated and characterized a full length cdna clone encoding the precursor of the human heart mitochondrial phosphate carrier protein. the entire clone is 1330 bp in length with 5'- and 3'-untranslated regions of 48 and 184 bp, respectively. the open reading frame encodes the mature protein consisting of 312 amino acids, preceded by a presequence of 49 amino acids. the amino acid sequence of the mature human phosphate carrier is 93.6, 94.2 and 33.6% identical to that of the phosphate car ... | 1991 | 1777677 |
| the tissue-specific mammalian transcription factor, pit-1, activates transcription in saccharomyces cerevisiae. | pit-1 is a tissue-specific transcription factor which binds to specific dna sequences within 5' flanking regions of the prl and gh genes and activates the transcription of these genes. previous studies have shown that expression of pit-1 is necessary to activate transcription from the prl or gh promoters in heterologous mammalian cells. in the present study the ability of pit-1 expression vectors to activate expression of reporter genes in saccharomyces cerevisiae was examined. the test system u ... | 1991 | 1770950 |
| molecular cloning of soluble aminopeptidases from saccharomyces cerevisiae. sequence analysis of aminopeptidase yscii, a putative zinc-metallopeptidase. | plasmids capable of complementing lap1, lap2 and lap3 mutations [r.j. trumbly and g. bradley (1983) j. bacteriol. 156, 36-48] were isolated from a yeast yep13 library by screening for activity against the chromogenic aminopeptidase substrate l-leucine beta-naphthylamide in intact yeast colonies. the genomic inserts were shown to contain the structural genes for aminopeptidases yscii, ysciii and ysciv. plasmids containing the gene encoding aminopeptidase yscii of saccharomyces cerevisiae, ape2 (l ... | 1991 | 1765107 |