Publications

changes in the intestinal flora of young pigs with postweaning diarrhea or edema disease.		1979	393690
the binding of lps to the lymphocyte surface.	bacterial lipopolysaccharide (lps) from escherichia coli labelled with tritium has been used to follow the binding of lps to lymphocytes. binding to cells rose to a maximum 2-7 min after addition of [3h]-lps, followed by loss of [3h]-lps from cells, reducing to about 10% of the peak level at 20-30 min. peripheral blood lymphocytes, mesenteric lymph node and thymus cells of the pig and cba, c3h/he and c3h/hej mouse spleen cells all bound [3h]-lps transiently at similar levels. it is concluded tha ...	1979	391698
the ability of cholestyramine resin and other adsorbents to bind escherichia coli enterotoxins.	several adsorbent materials were evaluated for their ability to bind escherichia coli enterotoxins. cholestyramine, a strong anion-exchange resin, bound the heat-labile and the heat-stable types of enterotoxin and reduced significantly their effects in some animal models. however, its efficacy in the treatment of diarrhoeic piglets appeared to be adversely affected by the presence of milk in the alimentary tract.	1979	390155
[effect of reduced gastrointestinal motility on the regulation of gastrointestinal flora and the pathogenesis of coli enterotoxinemia in market swine].	opium tincture and spasmentral were applied to piglets early after weaning and reduced their gastro-intestinal motility, which, however, caused only very minor changes in quantitative germ flora composition in those first days. short-time suppression of gastro-intestinal motility obviously does not result in detrimental consequences to the organism as a whole, since there seem to be several factors which are involved in the control and regulation of the intestinal germ flora. impairment of gastr ...	1979	389190
determinants of the action of phospholipases a on the envelope phospholipids of escherichia coli.		1979	387763
relationship between the intestinal permeability to macromolecules and invasion of septicemia-inducing escherichia coli in neonatal piglets.	the influence of age and diet on the invasion of septicemia-inducing escherichia coli and the endocytotic activity of the small intestinal epithelium were examined in colostrum-deprived conventional and gnotobiotic piglets orally infected with e. coli 078. the piglets infected at birth and the animals fed glucose-amino acids solution and infected at 3 days after birth soon suffered from septicemia caused by the invasion of e. coli 378. the piglets fed artifical milk and infected at 3 days after ...	1979	387609
aspartate transaminase from e. coli: amino acid sequences of the nh2-terminal 33 residues and chymotryptic pyridoxyl tetrapeptide.		1979	387032
the role of thromboxane a2 in endotoxin-induced aggregation of guinea-pig platelets in vitro.	large concentrations of endotoxin lipopolysaccharides (lps) e. coli o127:b8 and e. coli o26:b6 were needed for induction of platelet aggregation in citrated platelet rich plasma (prp) from normal guinea-pigs. when guinea-pigs were actively immunized with lps e. coli o127:b8 their prp became more sensitive to the aggregatory effects of this type of lps. the increase in sensitivity was rather selective since the responses to adp and lps e. coli o26:b6 remained unchanged. in endotoxin-stimulated pr ...	1979	386740
survival times of bacteria in liquid manure.		1977	382807
immunological studies in mice with swine edema disease principle (neurotoxin).		1979	382704
renal damage due to urinary tract infection.	chronic pyelonephritis is a disease of childhood which is carried on into adult life. children under the age of 4 in whom uti, vesico-ureteric reflux and pyelotubular back-flow coexist appear to be particularly at risk of developing kidney damage. attempts to prevent kidney damage in adults by detection and treatment of covert uti are doomed to failure because persistent bacteriuria in the adult is a consequence of underlying abnormality of the urinary tract rather than its cause. our main hope ...	1979	380901
effect of circulating, maternally derived antibody on the development of a local immune response in the intestine of the neonatal pig.		1979	378047
influence of o and k antigens on the surface properties of escherichia coli in relation to phagocytosis.	strains of escherichia coli with different o and k antigens were investigated with respect to physicochemical surface characteristics and liability to phagocytosis. using two-phase partitioning analysis for the surface characterization, three main groups of strains emerged: group i (o1:k1, o2:k1, o3:k2ab) showing both smooth hydrophilic o antigens and negatively-charged k antigen which rendered the strains maximally resistant to phagocytosis. group ii (o55:k59, o111:k58) showed no acidic k antig ...	1979	375674
[evaluation of the efficacy of carbadox as a growth stimulant by microbiological criteria].		1979	375628
[cerebrospinal angiopathy after experimental protracted shock (author's transl)].		1978	371211
detection of iga anti-escherichia coli plasma cells in the intestine and salivary glands of pigs orally and locally infected with e. coli.		1978	369316
studies on escherichia coli in pigs v. determination of enterotoxicity and frequency of o groups and k 88 antigen in strains from 200 piglets with neonatal diarrhoea.		1978	369239
[production of live attenuated vaccine against colibacillosis in pigs].	two strains of beta-hemolytic escherichia coli, p-0141 and i-0149 were used to obtain mutant e. coli organisms with unchanged cultural, morphological, biochemical, and serological properties. the mutants proved apathogenic for albino mice at intravenous injection of a 6-hour broth culture at the rate of 0.3 cm3 or an endotoxin in the same dose. neither were they enteropathogenic for pigs when inoculated into intestinal segments. they could be used as vaccinal strains.	1978	369110
the influence of plasmid-determined and other characteristics of enteropathogenic escherichia coli on their ability to proliferate in the alimentary tracts of piglets, calves and lambs.		1978	364065
superiority of human complement for assaying bacterial lipopolysaccharides by their anticomplementary activity.	in assaying bacterial lipopolysaccharides (lps) for anticomplementary activity, human complement (c) allowed detection of approximately 200 times smaller amounts of lps than guinea-pigs c. pig c was slightly inferior to human.	1978	363447
proteinase, lipase and amylase activities in the small intestine of pigs suffering from colienterotoxaemia.		1978	358811
[natural antibodies (isohemagglutinins, heterophilic, antierythrocytic and antibacterial) in children with congenital immunologic deficiency syndromes].	examination of 30 patients with immunodeficient diseases showed that the system of natural antibodies was considerably changed depending on the form and the extent of deficiency of the immune system: in agammaglobuinemia the antibodies under study were almost completely absent, and in immunological insufficiency with ataxia-teleangiectasis the production of antibacterial antibodies proved to be sharply decreased. the data obtained can be used both for the diagnosis of immunodeficiencies and for ...	1978	358690
[various aspects of competitive relationship between shigella and escherichia coli].	one of the aspects of antagonistic relations could be the competence of microorganisms for specific sites of attachement to the epithelium common for shigellae and some e. coli serological types; this was demonstrated on a model of shigella-induced keratoconjunctivitis and in experiments with the hep-2 cells infected with h3-glucose-labeled sh. flexneri 5a, with combined administration of the latter with e. coli 08 and 028. the data obtained emphasized the importance for the development of the i ...	1978	358685
biological evaluation of a methanol-soluble, heat-stable escherichia coli enterotoxin in infant mice, pigs, rabbits, and calves.	escherichia coli p16 was shown to produce two heat-stable toxins (st) with differing biological activity. the toxins were separated by methanol extraction, and the first, sta, was methanol soluble, partially heat stable, active in neonatal piglets (1 to 3 days old) and infant mice, but inactive in weaned pigs (7 to 9 weeks old); the second, stb, was methanol insoluble, active in weaned pigs and rabbit ligated loops, but inactive in infant mice. it is therefore suggested that use of suckling mice ...	1978	357288
in vitro adhesion of escherichia coli to porcine small intestinal epithelial cells: pili as adhesive factors.	escherichia coli strains with pili (k99 or 987p) known to facilitate intestinal colonization adhered in vitro to porcine intestinal epithelial cells. these strains adhered equally to both ileal and jejunal epithelial cells. a laboratory e. coli strain that has type 1 pili also adhered to porcine intestinal epithelial cells. when nonpiliated cells derived from 987p+, k99+, or type 1 pilus+ strains were used for in vitro adhesion assays, they failed to adhere. the attachment of piliated bacteria t ...	1978	357285
effect of heat stable and heat labile escherichia coli enterotoxins and cholera toxin in combination with theophylline on unidirectional sodium and chloride flux in the small intestine of weanling swine.	the effect of heat stable and heat labile escherichia coli enterotoxins or cholera toxin in combination with theophylline on net water, sodium and chloride and unidirectional sodium and chloride fluxes was examined in acute isolated loops of jejunum of weanling swine. the effect of heat stable enterotoxin in combination with theophylline was determined in loops located in the proximal jejunum, while combinations of theophylline and either heat labile enterotoxin or cholera toxin were studied in ...	1978	356942
characterization of plasmids that encode for the k88 colonization antigen.	k88 antigen, and important virulence factor in porcine enteropathogenic escherichia coli (eec), can be transferred along with the ability to ferment the trisaccharide raffinose (raf). the plasmids from a number of eec strains that encode these two properties were isolated and characterized. in most strains the k88 and raf genes were found on a single nonconjugative plasmid approximately 50 x 10(6) daltons in size. this plasmid core was conserved with only slight variation among the strains teste ...	1978	352948
hydrogen sulphide-positive strains of escherichia coli from swine.	thirty-two h2s-positive strains of escherichia coli, isolated from the caecal contents or mesenteric lymph nodes or both of 60 apparently healthy pigs, were characterized. eighteen different serotypes and 18 different fermentative types were found. during conjugation with e. coli k12, four strains transferred their hys plasmids linked to raf, and another transferred hys associated with the tc determinant. all hys-raf plasmids belong to incompatibility group fi, whilst tc-hys is an fi- plasmid.	1978	351185
[animal tissue polynucleotide phosphorylase].	localization, physico-chemical and catalytic properties and possible biological functions of polynucleotide phosphorylase (pnpase) from animal tissues are discussed. in animal tissue cells pnpase has multiple localization; the major amount of the enzyme is localized in the endoplasmic reticulum ribosomes. in the nuclei pnpase, similar to other endo- and exo-rnases participates in the processing of precursor molecules of mature forms of rna, whereas in the cytoplasm it is involved in the destruct ...	1978	350293
microbial iron-chelators and their action on klebsiella infections in the skin of guinea-pigs.	preparations of catechols from ethyl acetate extracts of cultures of klebsiellae in a low-iron medium contained iron-chelators whose potency was measured by the reversal of the bacteristasis of escherichia coli and klebsiellae in unheated horse serum, and of the growth-inhibition of these two organisms by ethylene diamine di-orthohydroxyphenyl acetic acid (edda). as revealed by in situ tests of paper chromatograms, there was a multiplicity of biologically active chelators in the preparations. ca ...	1978	350255
purification and properties of a pig heart thiolase with broad chain length specificity and comparison of thiolases from pig heart and escherichia coli.	a thiolase (acetyl coa acyltransferase, ec 2.3-1.16) which acts on substrates of various chain lengths (thiolase i) has been purified from pig heart muscle 366-fold to near homogeneity as judged by gel electrophoresis. its molecular weight was estimated to be 200,000 in the absence and 46,000 in the presence of sodium dodecyl sulfate. kinetic measurements with acetoacetyl coenzyme a, 3-ketohexanoyl-coa, 3-ketooctanoyl-coa, and 3-ketodecanoyl-coa yielded apparent km values of 16, 8.3, 2.4, and 1. ...	1978	344310
detecting enteropathogenic escherichia coli strains of porcine origin. 1. correlations between o and k antigens and the enterotoxin production in strains isolated from the newborn piglet.	a study of enterotoxin production by means of biological tests (y1 adrenal cells and suckling mice) using 67 escherichia coli strains responsible for diarrhea in newborn piglets, confirmed the advantages of these methods and demonstrated the correlation between antigenic specificity and enterotoxin production. in particular the presence of capsular antigen k 88 is related to the thermolabile (lt) fraction of the enterotoxin in 73.9 % of the enteropathogenic strains studied.	1977	343700
alternatives to aspirin, derived from biological sources.		1977	343554
the sources of "oh" serotypes of escherichia coli.	the sources from which different "oh" serotypes of escherichia coli have been isolated are listed in a series of tables. it is considered that these tables should provide a basis for obtaining a greater understanding of the ecology of e. coli.	1978	340583
effects of various nutrient levels and enviromental temperatures on the incidence of colibacillary diarrhea in pigs: intestinal fistulation and titration studies.		1977	340437
uptake of maternal antibody by the neonatal pig following intramuscular and intramammary vaccination of the preparturient sow.	administration of heat inactivated escherichia coli antigens by intramuscular and intramammary routes induced elevated antibody levels in sow serum and colostrum, predominantly associated with igg. colostral igg accounted for approximately 80 per cent of the total antibody activity, and there was a similar distribution in the sera of one-day-old piglets. the additional antibody activity was carried almost entirely by igm following intramuscular injections and was evenly distributed between igm a ...	1977	337428
human milk and gut infection in the newborn.		1977	336123
breeding record analysis in pig herds and its veterinary applications--2: experiences with a large commercial unit.	a computer program was used to analyse data from a large commercial pig unit over two years to assess reproductive efficiency and the efficiency of weaner production. monthly inspections of the farm and quarterly post mortem examinations of all dead pigs provided further information. the effects of controlled changes in management on production were assessed. overall the number of piglets reared per sow per year was increased from 15-1 to 17-2 and the eight-week weights improved from a mean of 1 ...	1977	335631
effect of heat stable and heat labile escherichia coli enterotoxins, cholera toxin and theophylline on unidirectional sodium and chloride fluxes in the proximal and distal jejunum of weanling swine.	acute, isolated loops of proximal and distal jejunum of weanling swine were exposed to either heat stable porcine escherichia coli enterotoxin, heat labile porcine escherichia coli enterotoxin, cholera toxin or theophylline. unidirectional sodium fluxes in response to heat stable in the proximal jejunum were dependent on the length of time that the intestinal mucosae was exposed to the enterotoxin. net water, sodium and chloride and unidirectional sodium and chloride flux measurements in the pro ...	1977	332292
[enterotoxin-producing e.-coli bacteria in enteritis and edema disease of swine. quick diagnosis of heat-labile and heat resistant toxin].		1977	332152
[experimental studies on the pathogenesis of coli-enterotoxemia in swine. 4. effect of lipopolysaccharide endotoxin on weaned piglets following parenteral administration].	ten clinically intact weaned piglets were experimentally intoxicated by intravenous injection of lipoproteide-free lipopolysaccharide endotoxin according to westphal of e. coli o 127:b8. severe endotoxin shock with all clinical manifestations of experimental coli-enterotoxaemia was induced in all animals and included circulatory disorder with tachycardia, intermittent pallor and/or cyanosis, symptoms of severe systemic intoxication, neurological symptoms, such as lack of coordination, hindleg st ...	1977	332109
a microbiological study of gastro-enteritis in black infants.	a survey was carried out to determine the aetiological agents responsible for acute gastro-enteritis in black infants. bacteriological examination of 191 patients and 178 age-matched controls showed that salmonellae and shigellae together accounted for only 15% of cases and were harboured by almost 7% of controls. seropositive 'enteropathogenic' escherichia coli was an important cause of diarrhoea, especially in the summer months. enterotoxin-producing e. coli was not a significant cause of gast ...	1977	331509
r-factor-mediated antibiotic resistance in e. coli strains isolated from piglets in sweden.	e. coli strains, isolated from piglets over the periods 1964-1968 and 1974-1975, were investigated and compared with respect to antibiotic resistance. the frequency of monoresistant strains decreased from 50% in 1964-68 to 27% in 1974-75, while that of strains showing double or multiple resistance increased from 13% to 22%. the proportions of the different resistance determinants were as follows (figures for 1964-68 within parentheses): tetracycline 23% (49%), sulphaisodimidine 38% (24%), strept ...	1977	329617
reduction of reactivity of escherichia coli enterotoxins by intestinal mucosal components.	incubation studies involving rabbit and piglet small intestinal mucosal components and escherichia coli and vibrio cholerae enterotoxins were conducted at 37 and 4 degrees c. mucosal homogenate cytosol from rabbits did not significantly alter the reactivities of either cholera enterotoxin (ct) or e. coli labile enterotoxin (lt). however, mucosal homogenate cytosol from piglets was capable of neutralizing lt, though it had no effect on e. coli stable enterotoxin. lt became bound to piglet and rab ...	1977	326677
interactions of radio-detoxified escherichia coli endotoxin preparations with the complement system.	escherichia coli o89 lipopolysaccharide (lps) was treated with different doses of gamma irradiation (5, 10, 15, and 20 mrad). various biological activities such as lethal effect, decrease in arterial blood pressure in dogs, and interaction with the complement system were determined for the parent and irradiated preparations. irradiation of lps significantly and in a dose-dependent manner decreased its lethal and blood pressure-depressing effects along with its ability to activate the complement ...	1977	326671
[comparative study of the virulence of enteropathogenic intestinal bacilli 0124 and crimea].		1977	325332
colonization of porcine small intestine by escherichia coli: colonization and adhesion factors of pig enteropathogens that lack k88.	the colonizing and adhesive attributes of enterotoxigenic acapsular and/or nonpiliated mutants from k88-negative enteropathogenic escherichia coli strains were compared with their capsulated and piliated parents (parents were piliated when grown in vitro and in vivo). acapsular, nonpiliated mutants from three different colonizing strains of enteropathogenic e. coli lost their ability to colonize the ileum of newborn pigs. acapsular, piliated and capsular, nonpiliated mutants were derived from on ...	1977	323375
[coagulation analytical studies in coli enterotoxemia in swine].	store pigs with spontaneous outbreaks and experimental endotoxin shock were kept under observation in the context of coagulation analysis. heparin was applied to some of the animals to disrupt the plasmatic coagulation system. the thrombocyte count in animals with endotoxin infusion declined by some 50 to 65 percent of the original level. no statistically secured difference was found to exist between heparinised animals, on the one hand, and non-heparinised, on the other. the aggregation and adh ...	1977	322631
occurrence of k99 antigen on escherichia coli isolated from pigs and colonization of pig ileum by k99+ enterotoxigenic e. coli from calves and pigs.	several strains of enterotoxigenic escherichia coli (etec) isolated from pigs were found to have an antigen (k99) previously reported only on strains of calf and lamb origin and which facilitates intestinal colonization in the latter two species. several human etec were also tested for k99; however, none were positive. each of four k99-positive etec strains of calf origin and one of pig origin produced k99 in pig ileum in vivo, adhered to villous epithelium in pig ileum, colonized pig ileum, and ...	1977	321356
[influence of cleansing and disinfection of drinking troughs on germ content and coli titer of drinking water].		1977	319787
a cyclic adenosine 3',5'-monophosphate-dependent histone kinase from pig brain. purification and some properties of the enzyme.	a cyclic adenosine 3',5'-monophosphate-dependent histone kinase (atp: protein phosphotransferase, ec 2.7.1.37) was isolated from pig brain. the enzyme has been purified 1140-fold; it is homogeneous on polyacrylamide gel electrophoresis and gel filtration. the estimated molecular weight of the enzyme is 120 000. histone kinase dissociates into a catalytic subunit and a regulatory one (molecular weights 40 000 and 90 000, respectively). the catalytic subunit has been obtained in homogeneous state ...	1975	235302
the aetiology of diarrhoea in pigs weaned at two days of age.	when pigs are weaned at two days of age large numbers of excherichia coli appear in the anterior gut and the incidence of diarrhoea rises. the two phenomena do not appear to be directly related because the strains of e coli isolated are not serotypes previously found to be associated with neonatal pig scouring. representative strains of the non-enteropathogenic serotypes did not produce enterotoxin and did not adhere to small intestine brush borders. moreover when antibiotics were fed to elimina ...	1979	228367
evidence for bactericidal activity of polymorphonuclear leukocytes without phagocytosis.	the relationship between phagocytosis and bactericidal action of polymorphonuclear leukocytes was examined by comparing the functions of cytochalasin d-treated leukocytes with those of the control. measurement of phagocytotic and bacterial dna-degrading activities using escherichia coli prelabeled with [3h]thymidine revealed that phagocytosis and bacterial dna degradation were inhibited by treatment with cytochalasin d to about 50 and 10% of the control, respectively. nevertheless, the bacterici ...	1979	225308
contamination of the deep tissues of carcasses by bacteria present on the slaughter instruments or in the gut.		1979	222722
elisa: enzyme-linked immunosorbent assay.	similar in design to radioimmunoassay, comparable in sensitivity and specificity but easier, safer, and less expensive, this new diagnostic technique uses enzyme-labeled rather than isotope-labeled reagents. the end point is a color change that can be assessed by colorimetry or with the naked eye. various techniques of elisa are described, along with examples of current and potential clinical applications.	1978	215505
naturally occurring and experimentally induced rotaviral infections of domestic and laboratory animals.		1978	212392
panel report on the colloquium on selected diarrheal diseases of the young.		1978	212391
the effect of cholera toxin and heat labile and heat stable escherichia coli enterotoxin on cyclic amp concentrations in small intestinal mucosa of pig and rabbit.	the effect of cholera toxin, heat labile and heat stable escherichia coli enterotoxin on mucosal cyclic amp concentrations was determined on the proximal jejunum of weanling pigs and young rabbits. ligated loops were injected with solutions containing no enterotoxin for control and either cholera toxin, heat labile or heat stable e. coli enterotoxin. the loops were drained after either two, four or six hours incubation at which time accumulated fluid was recorded and mucosal samples removed for ...	1978	210913
study of the in vitro pharmacological effects of gram-negative endotoxins in parallel with toxicity testing in normal adult guinea-pigs.		1978	206397
mechanisms in the pathogenesis of diarrhea: a review.		1978	203563
electron paramagnetic resonance spectra of myeloperoxidase in polymorphonuclear leukocytes.		1978	202508
preweaning mortality in the pig. pathological findings in piglets dying between birth and weaning.	during a survey of preweaning mortality 538 piglets which died between birth and weaning were autopsied. the results of laboratory examinations permitted a division of the findings into a number of syndromes which were considered to be associated with the immediate cause of death. the non-infectious conditions such as trauma, starvation and suffocation were the most common. seventy-eight piglets were included in the trauma group because of depressed cranial bone fractures or visceral rupture lea ...	1977	200212
gnotobiotic pigs-derivation and rearing.	the procurement, rearing, nutrition and microbiological monitoring of gnotobiotic pigs and a method for conditioning of primary, colostrum-deprived, specific pathogen free pigs is described. as compared to the established hysterectomy and closed hysterotomy methods for the derivation of gnotobiotic piglets an alternative approach, open caesarian section with the sow maintained under general halothane-nitrous oxide anaesthesia and the introduction of each fetus into the sterile isolator via a liq ...	1978	154359
comparison of the effect of various antisera and cobra venom factor on inflammatory reactions in guinea-pig skin. ii. the arthus reaction and the local shwartzman reaction.	the ability of antisera to guinea-pig c3 to inhibit the arthus and local shwartzman reactions was studied. they were found to reduce the non-haemorrhagic component of the active and reversed passive arthus reactions and to delay the appearance of the haemorrhage in the active arthus reaction. cobra venom factor, however, had no effect on the non-haemorrhagic components of these reactions and only delayed the appearance of the haemorrhage of the active arthus reaction. there appeared to be a corr ...	1975	125318
[observations on antibacterial activity of thiamphenicol glycinate acetylcysteinate (author's transl)].	in vitro thiamphenicol antibacterial activity in the presence of n-acetylcysteine as sodium salt proved to be enhanced when compared with that of the antibacterial alone against both gram-positive and gram-negative bacterial strains. the in vitro results were confirmed in vivo in the guinea-pig, where the sera from animals previously treated by i.m. route with thiamphenicol glycinate acetylcysteinate, showed a higher antibacterial activity than sera from animals treated with thiamphenicol glycin ...	1979	122158
[preliminary results of an atiepizootic field test using edta-na extract vaccine from escherichia coli strains pathogenic to swine in swine production industrial units].	an anti-epizootic field test was applied to industrialised pig farms in the region of wrocław, poland, to test the effectiveness of a pentavalent edta (calcium disodium edetate)--sodium vaccine extracted from escherichia coli strains with pathogenicity to swine. the vaccine had been received from a centre in berlin-buch, gdr. the vaccine failed to provide any protection, when orally applied to nursed piglets. however, both morbidity and mortality were reduced and, thus, an anti-epizootic effect ...	1979	121226
[demonstration of enterotoxin-forming pseudomonas aeruginosa strains in the intestinal ligature test in piglets].		1979	119390
incidence and relationship of group d streptococci with other indicator organisms in meats.	raw and processed meats were analyzed for presumptive group d streptococci using kf streptococcus agar. counts were compared with coliform, presumptive escherichia coli, and enterobacteriaceae counts but no meaningful relationships were observed. results indicated that group d streptococci and e. coli type i were principally contaminants from the packing plant, rather than at retail level. the predominating group d streptococcus in both beef and pork cuts was streptococcus faecalis, while in pro ...	1978	106944
mechanisms of immunity in bacterial infections.		1979	95156
relationships among raffinose plasmids determined by the immunochemical cross-reaction of their alpha-galactosidases.	plasmid-encoded alpha-galactosidase served as a marker enzyme for the recognition and comparison of raffinose (raf) plasmids present in strains of escherichia coli. immunochemical relationships were established among raf plasmids of 39 independent isolates from man and domestic animals (from three continents) by using antiserum against alpha-galactosidase. immunodiffusion revealed three serological subclasses of alpha-galactosidase, which are correlated with the biological and geographical origi ...	1979	94343
drug resistance in escherichia coli isolated from diarrhoeic piglets.		1979	92850
an examination of the o and k specificity involved in the antibody-induced loss of the k88 plasmid from porcine enteropathogenic strains of escherichia coli.	the heat-labile k88 antigen, a virulence determinant coded for by a transmissible plasmid, was eliminated from enteropathogenic strains of escherichia coli by passage through media containing antibodies to the heat stable antigens of an abbotstown (o149:k91,k88ac) strain. the plasmid-curing activity of o149 antisera was not o-antigen specific as o149, o45, o8 and o138 strains of e. coli could be 'cured' of their k88 plasmids by this technique. the curing activity was differentiated from the o-an ...	1979	92455
detecting enteropathogenic strains of escherichia coli of porcine origin. 2. relationship between o and k antigens and the production of enterotoxin in strains isolated from the piglet after weaning.	the production of enterotoxin by biological tests (yl adrenal cells and the suckling mouse) has been examined in 96 strains of escherichia coli, isolated from sick piglets after weaning. there is a good correlation between the presence of the capsular antigen k88 and that of the thermolabile fraction (lt) of the enterotoxin (69.2 per cent of the enteropathogenic strains studied). however, the presence of the thermostable fraction (st) of the enterotoxin of strains which, according to their serol ...	1978	86320
escherichia coli strains that cause diarrhoea but do not produce heat-labile or heat-stable enterotoxins and are non-invasive.	three enteropathogenic escherichia coli (e.p.e.c.) strains (o127:k63:h6, o128:k67:h2, and o142:k86:h6) isolated from outbreaks of infantile diarrhoea and one strain from the "normal" colonic flora (e. coli hs) of a healthy adult were fed in doses of 10(6), 10(8), and 10(10) organisms in nahco3 to adult volunteers. the strains, which had been stored for 7--9 years, gave negative results in sensitive tests for heat-labile (l.t.) enterotoxin (y-1 adrenal-cell test), heat-stable (s.t.) enterotoxin ( ...	1978	77415
immunological aspects of cell membrane adhesion demonstrated by porcine enteropathogenic escherichia coli.		1978	75179
clinical uses of kanamycin, neomycin and streptomycin.		1977	73127
mucosal adherence of human enteropathogenic escherichia coli.	an in-vitro assay system has been developed to measure bacterial adhesion to the mucosa of human fetal small intestine. two strains of escherichia coli that are proven human enteropathogens (e.p.e.c.) have been shown to adhere in large numbers, compared with control organisms. the attachment mechanism is species specific and is not caused by common fimbriae. mucosal adhesion may be as important as enterotoxin production or invasiveness in determining the virulence of some strains of human e.p.e. ...	1975	53431
an attempt to identify the intestinal receptor for the k88 adhesin by means of a haemagglutination inhibition test using glycoproteins and fractions from sow colostrum.	the k88 antigen of escherichia coli specifically adheres to the piglet intestinal cell; a solution of this antigen agglutinates guinea-pig red cells at 4 degrees c. the latter reaction was used as a model of the former, using inhibition of haemagglutination as an index of specific combination with the k88 adhesin. inhibition was found with mucous glycoproteins and chemical modification of their heterosaccharide residues by mild acid hydrolysis, periodate oxidation or the smith degradation proced ...	1975	46271
diastereoisomeric glucuronides of oxazepam. isolation and stereoselective enzymic hydrolysis.	oxazepam glucuronide isolated from swine urine by previously published methods was separated into its diastereoisomers by ion-exchange chromatography on a preparative scale. quantitative high-performance liquid chromatography was used to monitor the separation. the two isomers were obtained in analytically pure form and then characterized by elemental analysis, oxazepam content, mass spectrometry, ultraviolet spectroscopy, optical rotation and optical rotatory dispersion-circular dichroism. the ...	1979	35325
the occurrence of salmonellas, mycobacteria and pathogenic strains of escherichia coli in pig slurry.	ninety-eight samples of pig slurry from 54 farms were examined for the presence of salmonellas, porcine enteropathogenic strains of haemolytic escherichia coli and mycobacteria. salmonellas were isolated from 12 farms (22%) and enteropathogenic e. coli from 13 farms (24%). pathogenic mycobacteria were not isolated. salmonellas were isolated from 7 of 16 farms (44%) stocked with 'minimal disease' pigs compared with only 5 of 38 farms (13%) stocked with conventionally reared pigs. conversely enter ...	1976	10332