Publications

diheteropeptin, a novel substance with tgf-beta-like activity, produced by a fungus, diheterospora chlamydosporia. ii. physico-chemical properties and structure elucidation.	the structure of diheteropeptin (1), a tgf-beta-like active substance from diheterospora chlamydosporia q58044, was determined to be a new cyclotetrapeptide, cyclo[2aminoisobutyryl-(s)-phenylalanyl-(r)-prolyl-(2s,8r,9r)-2-am ino-8,9-dihydroxydecanoyl-] by nmr, mass spectrometric and chemical studies.	2000	11079801
diheteropeptin, a new substance with tgf-beta-like activity, produced by a fungus, diheterospora chlamydosporia. i. production, isolation and biological activities.	a new metabolite, diheteropeptin, was found in the culture broth of diheterospora chlamydosporia q58044 by screening for tgf-beta-like active substances. diheteropeptin was extracted from the culture supernatant and purified by a series of chromatographies such as silica gel, gel filtration and hplc. diheteropeptin exhibited cytostatic activity in mv1lu cells with an ic50 value of 20.3 microm and inhibited histone deacetylase.	2000	11079800
identification of peptide synthetase-encoding genes from filamentous fungi producing host-selective phytotoxins or analogs.	race 1 of cochliobolus carbonum (cc) makes a cyclic tetrapeptide, hc-toxin, that is necessary for its virulence on certain genotypes of maize. the synthesis of hc-toxin is catalyzed by a 570-kda multifunctional enzyme, hc-toxin synthetase (hts). the gene encoding hts (hts1) is absent from other races of cc and from other species of cochliobolus. four other unrelated filamentous fungi make cyclic peptides closely related to hc-toxin, raising the possibility that the corresponding cyclic peptide s ...	1995	8522177
the nematophagous fungus verticillium chlamydosporium produces a chymoelastase-like protease which hydrolyses host nematode proteins in situ.	the nematophagous fungus verticillium chlamydosporium secreted several proteases in submerged culture in which soya peptone was the sole carbon and nitrogen source. one protease, vcp1 (m(r) 33,000, pi 10.2), was purified 14-fold from culture filtrates to apparent homogeneity using preparative isoelectric focusing in free solution, and shown to rapidly hydrolyse the chymotrypsin substrate suc-(ala)2-pro-phe-pna and elastin. vcp1 had a km for suc-(ala)2-pro-phe-pna of 4.3 x 10(-5) m and a kcat of ...	1994	8000541
pcr primers that allow intergeneric differentiation of ascomycetes and their application to verticillium spp.	a pair of conserved pcr primers, designated nms1 and nms2, that amplify a region in the mitochondrial small rrna gene region were designed for fungi belonging to the class ascomycetes. these primers were tested with members of eight fungal genera (aspergillus, fusarium, magnaporthe, mycospharella, neurospora, saccharomyces, sclerotinia, verticillium) and 10 verticillium species (verticillium albo-atrum, verticillium chlamydosporium, verticillium cinnebarium, verticillium dahliae, verticillium fu ...	1994	7811072
the subtilisins of the invertebrate mycopathogens verticillium chlamydosporium and metarhizium anisopliae are serologically and functionally related.	the major extracellular proteases from the nematophagous fungus verticillium chlamydosporium and the entomophagous fungus metarhizium anisopliae, vcp1 and pr1, respectively, are closely related both functionally and serologically. antibodies raised against either enzyme cross-reacted with both antigens, suggesting that they have common epitopes. the vcp1 and pr1 antisera labelled bovine pancreatic elastase and proteinase k, respectively. neither antiserum reacted with commercial chymotrypsin. an ...	1995	7729666
the relation between proteolytic activity and ovicidal activity of mutants of verticillium chlamydosporium.		1984	6242627
penetration of ovicidal fungus verticillium chlamydosporium through the ascaris lumbricoides egg-shells.	the course of penetration of the ovicidal fungus verticillium chlamydosporium goddard through the ascaris lumbricoides egg-shells was studied in electron miorographs. the contact area between the egg and the fungus is smooth at the stage of the contact of the ovicidal fungus with the egg surface, as well as at the stage of adhesion. no special attaching organs are formed which indicates that no mechanical penetration of the fungal hypha through the egg-shells is involved. traces of enzymatical a ...	1987	3583130
mutants of ovicidal fungus verticillium chlamydosporium with changed chitinolytic activity.		1987	2962466
colonization of ascaris lumbricoides eggs by the fungus verticillium chlamydosporium goddard.	the process of colonization of ascaris lumbricoides eggs by the fungus verticillium chlamydosporium was studied by scanning electron microscopy. the preparations were made by fractionation of egg suspension exposed to the fungus for four days and frozen in liquid nitrogen according to stĕrba and milácek (1986). ovicidal fungus forms an abundant ramifying mycelial network in the area between the eggs. however, egg-shells are penetrated only by some hyphae without any penetration organs produced ( ...	1991	1808033
the development and structure of thick-walled, multicellular, aerial spores in diheterospora chlamydosporia (=verticillium chlamydosporium).	the aerial, thick-walled spores in diheterospara chlamydosporia arose as terminal swellings on erect hyphae. repeated septation of the continuously swelling spore resulted in a multicellular structure. immediately after the onset of septation secondary wall material was laid down between the two-layered primary wall and the plasmalemma. the presence of secondary wall material indicates that the multicellular spore is a dictyochlamydospore and not an aleuriospore. the relationship between chl ...	1975	1170933