Publications

immunofluorescence as an adjunct to the histopathologic diagnosis of chagas' disease.	we have developed a new indirect immunofluorescence procedure for identifying trypanosoma cruzi amastigotes in sections of formalin-fixed, paraffin-embedded tissue pretreated with a 0.25% trypsin solution to enhance immunofluorescence. in sections of human and mouse myocardia infected with t. cruzi and stained by this procedure, both intact amastigotes and phagocytosed amorphous antigen were intensely fluorescent and easily detected; nonspecific background fluorescence was absent or minimal. no ...	1988	3128582
hypercalcemia and disseminated histoplasmosis in an owl monkey.	an adult male owl monkey (aotus nancymai) had hypercalcemia associated with a disseminated case of histoplasmosis (histoplasma capsulatum var. capsulatum). histopathologic examination of tissues at necropsy showed moderate to marked numbers of small granulomas involving multiple organs. the granulomas contained numerous round, basophilic, intracellular structures, 1-2 microns in diameter, that stained positive in periodic acid-schiff and gomori's methenamine silver tests. the hypercalcemia in th ...	1990	2254918
histoplasmosis duboisii (african histoplasmosis). an african case reported from chile with ultrastructural study.	a case of histoplasmosis duboisii in a 30 year-old engineer is presented. the diagnosis was made with the help of light microscopy, electron microscopy and cultures. although diagnosed in chile, the patient probably acquired the disease in the endemic african area, more precisely in the ivory coast. differential diagnosis between histoplasma capsulatum var. capsulatum and histoplasma capsulatum var. duboisii is based primarily on the larger in-vivo yeast form size of the latter. electron microsc ...	1990	2247379
evidence that the m antigen of histoplasma capsulatum var. capsulatum is a catalase which exhibits cross-reactivity with other dimorphic fungi.	a panel of monoclonal antibodies (mabs) was raised against histoplasmin, the antigen derived from the mycelial phase of histoplasma capsulatum var. capsulatum which contains the diagnostically useful h and m antigens. a number of mabs were obtained which recognized a 70-75 kd component of an antigenic preparation of h. capsulatum var. capsulatum by western blotting. when reacted with histoplasmin by western blotting the mabs recognized a similar 70-75 kd band, together with a series of higher mo ...	1990	2093121
in vitro production of extracellular elastolytic proteinase by histoplasma capsulatum var. duboisii and histoplasma capsulatum var. capsulatum in the yeast phase.	extracellular elastolytic proteinase was produced by yeast cultures of histoplasma capsulatum var. duboisii and h. capsulatum var. capsulatum in three different induction media, assayed using elastin-orcein as the substrate. medium-dependent variations in the time-course for enzyme production were observed and no peak was recorded. the proteinases hydrolysed both casein and bovine serum albumin indicating a broad substrate specificity. both elastolytic proteinases had similar optimum ph (ph 8) a ...	1991	1824415
a murine monoclonal antibody exhibiting high species specificity for histoplasma capsulatum var. capsulatum.	a monoclonal antibody (mab) exhibiting a high degree of species specificity for the yeast phase of the dimorphic fungus histoplasma capsulatum was produced by a modification of the standard mab production protocol. the technique for generating mabs involved the use of the immunosuppressive drug cyclophosphamide to diminish the response in mice to immunodominant cross-reactive epitopes. this mab exhibited clear specificity and did not react by elisa with the closely related genera blastomyces, pa ...	1990	1691267
production of extracellular collagenolytic proteinases by histoplasma capsulatum var. duboisii and histoplasma capsulatum var. capsulatum in the yeast phase.	yeast cultures of histoplasma capsulatum var. duboisii and h. capsulatum var. capsulatum in collagen containing defined, semi-defined and complex media produced extracellular collagenolytic proteinases, assayed using 4-phenylazo-benzyloxycarbonyl-l-propyl-l-leucyl- glycyl-l-propyl-d-arginine, a specific collagenase substrate. significant levels of hydroxyproline were measured in the cultures and clear zones of hydrolysis were produced in collagen buffer agar by the crude enzyme preparations. hyd ...	1991	1668620
disseminated histoplasmosis in a badger (meles meles) in denmark.	we report the first case of disseminated histoplasmosis in an animal in scandinavia. yeast cells compatible with those of histoplasma capsulatum var. capsulatum were found in the skin, liver, spleen, a kidney, and a lymph node of a wild badger (meles meles). the diagnosis was confirmed by electron microscopy and immunofluorescence staining of the yeast cells in tissue sections.	1992	1642845
effect of successful treatment with amphotericin b on histoplasma capsulatum variety capsulatum polysaccharide antigen levels in patients with aids and histoplasmosis.	the purpose of this study was to establish the effect of induction and maintenance treatment with amphotericin b on levels of histoplasma capsulatum var. capsulatum polysaccharide antigen (hpa) in the urine and blood of patients with acquired immunodeficiency syndrome (aids) and disseminated histoplasmosis.	1992	1543199
comparative evaluation of a chemiluminescent dna probe and an exoantigen test for rapid identification of histoplasma capsulatum.	a chemiluminescent dna probe (accuprobe) assay developed by gen probe, inc., for the rapid identification of histoplasma capsulatum was evaluated and compared with the exoantigen test by using 162 coded cultures including histoplasma capsulatum var. capsulatum, histoplasma capsulatum var. duboisii, histoplasma capsulatum var. farciminosum, blastomyces dermatitidis, coccidioides immitis, paracoccidioides brasiliensis, and morphologically related saprobic fungi. each test uses a chemiluminescent, ...	1992	1452692
a medium for inducing conversion of histoplasma capsulatum var. capsulatum into its yeast-like form.	histoplasma capsulatum var. capsulatum is a dimorphic fungus that, under special conditions, converts from its more common mycelial form to a yeast-like form. achieving this conversion, however, has been problematical for researchers. the present study tested conversion rates in ten histoplasma capsulatum var. capsulatum strains using seven culture media, four of which were conventional and three novel. one of our novel media, mlgema, induced complete conversion of two strains within five days o ...	1992	1308555
specific immunological test for the rapid identification of members of the genus histoplasma.	a sensitive and specific immunological method was developed for rapid identification of the mycelial forms of histoplasma capsulatum var. capsulatum, h. capsulatum var. duboisii, and h. farciminosum and for separation of these pathogenic fungi from morphologically similar hyphomycetes and other fungal pathogens. this method is based on the fact that all of the histoplasma spp. produce h and m histoplasmin antigens, whereas the other fungi do not. inocula consisting of heavy mycelial growth from ...	1976	56339