Publications

TitleAbstractYear
Filter
PMID(sorted descending)
Filter
cytotoxic and antibiotic cyclic pentapeptide from an endophytic aspergillus tamarii of ficus carica.a new cyclic pentapeptide, disulfide cyclo-(leu-val-ile-cys-cys) (1), named malformin e, together with 13 known cyclic dipeptides, was isolated from the culture broth of endophytic fungus fr02 from the roots of ficus carica. the strain fr02 was identified as aspergillus tamarii on the basis of morphological characteristics and molecular analyses of internal transcribed spacer (its). their structures were determined by the combination of 1d and 2d nmr spectroscopy, hrms (esi), uv, and marfey's an ...201627147299
identification of different fusarium spp. in allium spp. in germany.in 2013 allium cepa bulbs from different fields in northern and southern germany, seeds and sets from onion breeders were analysed for infestation with fusarium species. the same investigation was done in 2014 with different edible allium spp. from local markets. different fusarium spp. were isolated and identified by morphological characterisation. 24 different fusarium spp. were identified. the diversity of fusarium spp. and the intensity of infestation was higher on edible bulbs compared to t ...201527141742
genotyping and in vitro antifungal susceptibility testing of fusarium isolates from onychomycosis in india.onychomycosis refers to fungal infection of the nail and is commonly caused by dermatophytes, while yeasts and non-dermatophytic molds (ndm) are increasingly recognized as pathogens in nail infections. the present study was done to delineate molecular epidemiology of fusarium onychomycosis in india. five hundred nail samples of indian patients clinically suspected of onychomycosis were subjected to direct microscopy and fungal culture. representative fusarium isolates were further identified to ...201627138574
epitypification of fusisporium (fusarium) solani and its assignment to a common phylogenetic species in the fusarium solani species complex.fusisporium solani was described as the causal agent of a dry rot of potato in germany in the mid 19th century. as fusarium solani, the species became known as a plurivorous plant pathogen, endophyte, decomposer, and opportunistic pathogen of humans and nutritional symbiont of insects. in parallel, it became evident that the morphologically defined species f. solani represents a phylogenetically and biologically complex group of often morphologically cryptic species that has come to be known in ...201627055573
etiology and epidemiological conditions promoting fusarium root rot in sweetpotato.sweetpotato production in the united states is limited by several postharvest diseases, and one of the most common is fusarium root rot. although fusarium solani is believed to be the primary causal agent of disease, numerous other fusarium spp. have been reported to infect sweetpotato. however, the diversity of fusarium spp. infecting sweetpotato in north carolina is unknown. in addition, the lack of labeled and effective fungicides for control of fusarium root rot in sweetpotato creates the ne ...201627050570
fusariosis in a patient with acute myeloid leukemia: a case report and review of the literature.fusarium spp. causes infections mostly in patients with prolonged neutropenia. we describe the case of a disseminated fusarium solani infection in a patient with acute myeloid leukemia which never reached complete remission during its clinical course. the patient had profound neutropenia and developed skin nodules and pneumonia in spite of posaconazole prophylaxis. f. solani was isolated from blood and skin biopsy, being identified from its morphology and by molecular methods. by broth dilution ...201627008433
synthesis and evaluation of ester derivatives of 10-hydroxycanthin-6-one as potential antimicrobial agents.as part of our continuing research on canthin-6-one antimicrobial agents, a new series of ester derivatives of 10-hydroxycanthin-6-one were synthesized using a simple and effective synthetic route. the structure of each compound was characterized by nmr, esi-ms, ft-ir, uv, and elemental analysis. the antimicrobial activity of these compounds against three phytopathogenic fungi (alternaria solani, fusarium graminearum, and fusarium solani) and four bacteria (bacillus cereus, bacillus subtilis, ra ...201627007362
two new polyhydroxysterols produced by fusarium solani, an endophytic fungus from chloranthus multistachys.a highly antagonistic endophytic fungus, designated strain cl39, was originated from the leaves of chloranthus multistachys collected in wulong of chongqing municipality of china in november 2015. the strain was identified as fusarium solani based on morphological characteristics, 5.8s gene and internal transcribed spacer sequence analysis. two new compounds, 2β, 9α-dihydroxy-5α-methoxyergosta-7, 22-diene (1), 2β, 6β-dihydroxy-5α-methoxyergosta-7, 22-diene (2) have been isolated from the culture ...201627000714
investigation of mycoviruses in endophytic and phytopathogenic strains of colletotrichum from different hosts.fungi belonging to the colletotrichum genus can be categorized as endophytic or phytopathogenic. these fungi can be infected by viruses, termed mycoviruses, which are know to promote hypovirulence in infected fungi. however, there are few studies that have described mycoviral infections of endophytes. the production of secondary metabolites by endophytes with antimicrobial potential in inhibiting numerous pathogens has gained increasing attention. the aim of the current study was to investigate ...201626985921
challenges in fusarium, a trans-kingdom pathogen.fusarium species are emerging human pathogens, next to being plant pathogens. problems with fusarium are in their diagnostics and in their difficult treatment, but also in what are actual fusarium species or rather fusarium-like species. in this issue guevara-suarez et al. (mycopathologia. doi: 10.1007/s11046-016-9983-9 , 2016) characterized 89 isolates of fusarium from colombia showing especially lineages within the fusarium solani and oxysporum species complexes to be responsible for onychomyc ...201626966007
novel taxa in the fusarium fujikuroi species complex from pinus spp.the pitch canker pathogen fusarium circinatum has caused devastation to pinus spp. in natural forests and non-natives in commercially managed plantations. this has drawn attention to the potential importance of fusarium species as pathogens of forest trees. in this study, we explored the diversity of fusarium species associated with diseased pinus patula, p. tecunumanii, p. kesiya and p. maximinoi in colombian plantations and nurseries. plants displaying symptoms associated with a f. circinatum- ...201526955193
genotyping of fusarium isolates from onychomycoses in colombia: detection of two new species within the fusarium solani species complex and in vitro antifungal susceptibility testing.fusariosis have been increasing in colombia in recent years, but its epidemiology is poorly known. we have morphologically and molecularly characterized 89 isolates of fusarium obtained between 2010 and 2012 in the cities of bogotá and medellín. using a multi-locus sequence analysis of rdna internal transcribed spacer, a fragment of the translation elongation factor 1-alpha (tef-1α) and of the rna-dependent polymerase subunit ii (rpb2) genes, we identified the phylogenetic species and circulatin ...201626943726
identification of the non-ribosomal peptide synthetase responsible for biosynthesis of the potential anti-cancer drug sansalvamide in fusarium solani.sansalvamide is a cyclic pentadepsipeptide produced by fusarium solani and has shown promising results as potential anti-cancer drug. the biosynthetic pathway has until now remained unidentified, but here we used an agrobacterium tumefaciens-mediated transformation (atmt) approach to generate knockout mutants of two candidate non-ribosomal peptide synthetases (nrps29 and nrps30). comparative studies of secondary metabolites in the two deletion mutants and wild type confirmed the absence of sansa ...201626936154
detection of human pathogenic fusarium species in hospital and communal sink biofilms by using a highly specific monoclonal antibody.the fungus fusarium is well known as a plant pathogen, but has recently emerged as an opportunistic pathogen of humans. habitats providing direct human exposure to infectious propagules are largely unknown, but there is growing evidence that plumbing systems are sources of human pathogenic strains in the fusarium solani species complex (fssc) and fusarium oxysporum species complex (fosc), the most common groups infecting humans. here, a newly developed fusarium-specific monoclonal antibody (mab ...201626914362
molecular phylogenetics and anti-pythium activity of endophytes from rhizomes of wild ginger congener, zingiber zerumbet smith.zingiber zerumbet, a perennial rhizomatous herb exhibits remarkable disease resistance as well as a wide range of pharmacological activities. towards characterizing the endophytic population of z. zerumbet rhizomes, experiments were carried out during two different growing seasons viz., early-june of 2013 and late-july of 2014. a total of 34 endophytes were isolated and categorized into 11 morphologically distinct groups. fungi were observed to predominate bacterial species with colonization fre ...201626867602
studying of cellular interaction of hairpin-like peptide ecamp1 from barnyard grass (echinochloa crusgalli l.) seeds with plant pathogenic fungus fusarium solani using microscopy techniques.an interaction of recombinant hairpin-like cationic peptide ecamp1 with conidia of plant pathogenic fungus fusarium solani at the cellular level was studied by a combination of microscopic methods. ecamp1 is from barnyard grass (echinochloa crusgalli l.), and obtained by heterologous expression in escherichia coli system. as a result, a direct relationship between hyphal growth inhibition and increasing active peptide concentration, time of incubation and fungal physiological condition has been ...201626855384
effect of fermentation parameters, elicitors and precursors on camptothecin production from the endophyte fusarium solani.volumetric productivity of camptothecin from the suspension culture of the endophyte fusarium solani was enhanced up to ∼152 fold (from 0.19 μg l(-1) d(-1) to 28.9 μg l(-1) d(-1)) under optimized fermentation conditions including initial ph (6.0), temperature (32 °c) and agitation speed (80 rpm) with (5% (v/v)) ethanol as medium component. among various elicitors and precursors studied, tryptamine (0.5 mm) as precursor and bovine serum albumin (bsa) (0.075 mm) as an elicitor added on day 6 of th ...201626851893
ag doped hollow tio2 nanoparticles as an effective green fungicide against fusarium solani and venturia inaequalis phytopathogens.chemical-based pesticides are widely used in agriculture to protect crops from insect infestation and diseases. however, the excessive use of highly toxic pesticides causes several human health (neurological, tumor, cancer) and environmental problems. therefore nanoparticle-based green pesticides have become of special importance in recent years. the antifungal activities of pure and ag doped (solid and hollow) tio2 nanoparticles are studied against two potent phytopathogens, fusarium solani (wh ...201626808118
structural analysis of glucosylceramides (glccer) from species of the pseudallescheria/scedosporium complex.glucosylceramides (glccer) are the main neutral glycosphingolipids expressed in fungal cells. in this work, glucosylceramides (glccer) were extracted from three strains of scedosporium (pseudallescheria) boydii, one strain of pseudallescheria ellipsoidea and one strain of pseudallescheria angusta and purified by several chromatographic steps. using high-performance thin layer chromatography (hptlc), we found a similarity between glccer obtained from all of the analysed strains. a detailed struct ...201626781373
fusarium solani endocarditis in an acute leukemia patient. 201626706407
fusarium paranaense sp. nov., a member of the fusarium solani species complex causes root rot on soybean in brazil.isolates of fusarium obtained from soybean plants showing symptoms of root rot collected in subtropical southern and tropical central brazil were characterized based on phylogenetic analyses, sexual crossing, morphology, and pathogenicity tests. a novel species within the fusarium solani species complex (fssc) causing soybean root rot is formally described herein as fusarium paranaense. this species can be distinguished from the other soybean root rot pathogens in the fssc, which are commonly as ...201626693684
[molecular identification and in vitro susceptibility of fusarium from fungal keratitis in central china].to detect the genotypes and in vitro antifungal susceptibility of fusarium isolated from patients with fungal keratitis in central china.201526693650
novel bio-essential metal based complexes linked by heterocyclic ligand: synthesis, structural elucidation, biological investigation and docking analysis.new series of bio-essential metal based complexes linked by schiff base ligand (l) and 2,2'-bipyridine (bpy) have been synthesized and characterized by diverse spectral techniques such as elemental analysis, magnetic susceptibility, molar conductivity measurements, ft-ir, uv-vis., (1)h nmr, (13)c nmr, epr and mass. the spectral data suggest that the metal complexes espouse octahedral geometry around the metal ions. interactions of the complexes with ct dna have been explored by electronic absorp ...201626690017
roles of triolein and lipolytic protein in the pathogenesis and survival of mycobacterium tuberculosis: a novel therapeutic approach.discovery of novel secreted enzymes and proteins in mycobacterium tuberculosis (m. tuberculosis) are imperative to understanding the pathogenic system for pathogenesis requires attention. till date, the groups of these secreted enzymes are not meaningfully characterized in terms of m. tuberculosis. in this way, cutinase, a small lipolytic protein, exists in both bacteria and fungi as well which have a potential catalytic activity. during our search, we have found a few genes of m. tuberculosis d ...201626679705
heterologous overexpression and biochemical characterization of the (galactophospho)lipase from fusarium solani in pichia pastoris that is expressed in planta.high-level extracellular production of fusarium solani (galactophospho)lipase, named fsl, was achieved using a pichia pastoris x33 expression system. the (galactophospho) lipase encoding gene was cloned into pgapzαa with the saccharomyces cerevisiae α-factor signal sequence by two different ways. the two constructs consist of an additional sequence of a (his)6-tag of the vector fused to the n-terminus of this enzyme (tfsl) while the other expression vector was constructed without any additional ...201626675137
pathogenic ability and saline stress tolerance of two fusarium isolates from odontesthes bonariensis eggs.several fungal species represent a potential risk to embryos of odontesthes bonariensis (cuvier and valenciennes, 1835), a euryhaline freshwater fish that lives in the pampean inland waters and has potential economic relevance.201626674586
multilocus phylogeny reveals an association of agriculturally important fusarium solani species complex (fssc) 11, and clinically important fssc 5 and fssc 3 + 4 with soybean roots in the north central united states.the fusarium solani species complex (fssc) includes important root pathogens of soybean in the united states, but the evolutionary lineages associated with soybean root rot are unknown. a multilocus phylogeny based on 93 isolates from soybean and pea roots from north dakota and minnesota revealed that root rot was associated with three known phylogenetic species, fssc 3 + 4 (=fusarium falciforme) (3 % of isolates), fssc 5 (60 %), fssc 11 (34 %), and one unknown species, fssc x (2 %). of these sp ...201626671414
evaluation of antifungal efficacy of 0.1% and 0.25% riboflavin with uva: a comparative in vitro study.antifungal efficacy of photochemical cross-linking (pack-cxl) with 0.1% and 0.25% riboflavin was evaluated with a comparative in vitro study.201626644282
secretome analysis of the mycoparasitic fungus trichoderma harzianum all 42 cultivated in different media supplemented with fusarium solani cell wall or glucose.trichoderma harzianum is a fungus well known for its potential as a biocontrol agent against many fungal phytopathogens. the aim of this study was to characterize the proteins secreted by t. harzianum all42 when its spores were inoculated and incubated for 48 h in culture media supplemented with glucose (glu) or with cell walls from fusarium solani (fscw), a phytopathogen that causes severe losses in common bean and soy crops in brazil, as well as other crop diseases around the world. trichoderm ...201626631988
effect of seed pelleting with biocontrol agents on growth and colonisation of roots of mungbean by root-infecting fungi.mungbean (vigna radiata (l.) wilczek) is a leguminous pulse crop that is a major source of proteins, vitamins and minerals. root-infecting fungi produce severe plant diseases like root rot, charcoal rot, damping-off and stem rot. the soil-borne pathogens can be controlled by chemicals, but these chemicals have several negative effects. use of microbial antagonist such as fungi and bacteria is a safe, effective and eco-friendly method for the control of many soil-borne pathogens. biological contr ...201626619828
pseudomonas induces salinity tolerance in cotton (gossypium hirsutum) and resistance to fusarium root rot through the modulation of indole-3-acetic acid.abiotic stresses cause changes in the balance of phytohormones in plants and result in inhibited root growth and an increase in the susceptibility of plants to root rot disease. the aim of this work was to ascertain whether microbial indole-3-acetic acid (iaa) plays a role in the regulation of root growth and microbially mediated control of root rot of cotton caused by fusarium solani. seed germination and seedling growth were improved by both nacl and mg2so4 (100 mm) solutions when treated with ...201526587006
expression of vitamin d receptor and cathelicidin in human corneal epithelium cells during fusarium solani infection.to observe the expression of vitamin d receptor (vdr) in human specimen and immortalized human corneal epithelium cells (hcec) when challenged with fusarium solani. moreover, we decided to discover the pathway of vdr expression. also, we would like to detect the expression of cathelicidin antimicrobial peptide (camp) in the downstream pathway of vdr.201526558193
fatal cases of bloodstream infection by fusarium solani and review of published literature.fusarium species are ubiquitously present in environment and are well known as human pathogens with high mortality rate in immunocompromised patients. we report here two cases where immunocompromised patients developed fatal bloodstream infections by this organism. isolates were further identified by its1 region sequencing which confirmed them as fusarium solani. antifungal susceptibility testing was done following clsi m38-a2 guidelines to amphotericin b, fluconazole, itraconazole, voriconazole ...201626541869
the fusarium solani species complex: ubiquitous pathogens of agricultural importance.members of the fusarium solani species complex (fssc) are capable of causing disease in many agriculturally important crops. the genomes of some of these fungi include supernumerary chromosomes that are dispensable and encode host-specific virulence factors. in addition to genomics, this review summarizes the known molecular mechanisms utilized by members of the fssc in establishing disease.201626531837
antagonistic activities of novel peptides from bacillus amyloliquefaciens pt14 against fusarium solani and fusarium oxysporum.bacillus species have recently drawn attention due to their potential use in the biological control of fungal diseases. this paper reports on the antifungal activity of novel peptides isolated from bacillus amyloliquefaciens pt14. reverse-phase high-performance liquid chromatography revealed that b. amyloliquefaciens pt14 produces five peptides (pt14-1, -2, -3, -4a, and -4b) that exhibit antifungal activity but are inactive against bacterial strains. in particular, pt14-3 and pt14-4a showed broa ...201526496638
[identification of pathogens causing root rot disease on ligusticum chuanxiong in sichuan].to identify the pathogens causing root rot disease on ligusticum chuanxiong from the main producing areas in sichuan, and to provide gist of management of this disease in the future.201526495640
fungal infections after boston type 1 keratoprosthesis implantation: literature review and in vitro antifungal activity of hypochlorous acid.to review the current literature describing cases of fungal keratitis and endophthalmitis after boston keratoprosthesis (kpro) implantation and to characterize the antifungal activity of 0.01% hypochlorous acid against medically relevant fungi.201526488624
synthesis of heterocycle-attached methylidenebenzenesulfonohydrazones as antifungal agents.a series of heterocycle-attached methylidenebenzenesulfonohydrazone derivatives were synthesized and evaluated for their antifungal activities against seven phytopathogenic fungi such as fusarium graminearum, alternaria solani, valsa mali, phytophthora capsici, fusarium solani, botrytis cinerea, and glomerella cingulata. compounds 7b, 8d, 9a, 9b and 9d exhibited a good and broad-spectrum of antifungal activities against at least five phytopathogenic fungi at the concentration of 100 μg/ml. it de ...201526471091
phylogenetic analysis of fusarium solani associated with the asian longhorned beetle, anoplophora glabripennis.culture-independent analysis of the gut of a wood-boring insect, anoplophora glabripennis (coleoptera: cerambycidae), revealed a consistent association between members of the fungal fusarium solani species complex and the larval stage of both colony-derived and wild a. glabripennis populations. using the translation elongation factor 1-alpha region for culture-independent phylogenetic and operational taxonomic unit (otu)-based analyses, only two otus were detected, suggesting that genetic varian ...201226467954
crystal structure of an antifungal osmotin-like protein from calotropis procera and its effects on fusarium solani spores, as revealed by atomic force microscopy: insights into the mechanism of action.cposm is an antifungal osmotin/thaumatin-like protein purified from the latex of calotropis procera. the protein is relatively thermostable and retains its antifungal activity over a wide ph range; therefore, it may be useful in the development of new antifungal drugs or transgenic crops with enhanced resistance to phytopathogenic fungi. to gain further insight into the mechanism of action of cposm, its three-dimensional structure was determined, and the effects of the protein on fusarium solani ...201526456062
etiological analysis of fungal keratitis and rapid identification of predominant fungal pathogens.fungal keratitis is a worldwide-distributed refractory and potentially blinding ocular infection caused by various fungi. it is necessary to investigate the etiological and epidemiological characteristics of this disease and establish a rapid and specific pathogenic identification method. here, we isolated and identified fungal pathogens of 275 patients with presumed fungal keratitis from jilin province, china, and conducted statistical analyses of epidemiological information. the positive rate ...201626446032
molecular identification of fusarium species isolated from transgenic insect-resistant cotton plants in mexicali valley, baja california.cotton production in the mexicali valley is adversely affected by wilt and root rot disease associated with fusarium species. in the present study, we sought to isolate and identify the fusarium species in the rhizosphere of transgenic insect-resistant cotton plants grown in the mexicali valley. our analyses isolated four native fungi from the rhizosphere of cotton plants, namely, t-ica01, t-ica03, t-ica04, and t-ica08. these fungal isolates were categorized as belonging to fusarium solani using ...201526436498
response surface optimization for decaffeination and theophylline production by fusarium solani.coffee processing industries generate caffeine-containing waste that needs to be treated and decaffeinated before being disposed. five fungal isolates obtained on caffeine-containing mineral media were tested for their ability to utilize caffeine at high concentrations. an isolate identified as fusarium solani could utilize caffeine as a sole source of carbon and nitrogen up to 5 g/l and could degrade it to an extent of 30-53 % in 120 h. sucrose that was added as an auxiliary substrate (5 g/l) e ...201626419659
cluster of fusarium solani isolations in a bronchoscopy unit. 201626417853
ruta montana l. leaf essential oil and extracts: characterization of bioactive compounds and suppression of crown gall disease.the aims of this study were to assess the antimicrobial efficacy of the leaf essential oil and the leaf extracts of r. montana against botrytis cinerea, fusarium oxysporum, verticillium dahliae, aspergillus oryzae and fusarium solani. the oil (1.000 µg/disk) and the extracts (1.500 µg/disk) revealed a remarkable antifungal effect against the tested plant pathogenic fungi with a radial growth inhibition percentage of 40.0-80.0 % and 5.0-58.0 %, respectively along with their respective mic values ...201526417353
cd34+ highly enriched allogeneic stem cell transplantation in a patient with mixed phenotype acute leukemia and fusarium solani sepsis. 201626382275
role of antagonistic microorganisms and organic amendment in stimulating the defense system of okra against root rotting fungi.without application of chemical pesticides control of soilborne diseases is a great challenge. stimulation of natural plant's defense is considered as one of the most promising alternative strategy for crop protection. organic amendment of soil besides direct suppressing the pathogen, has been reported to have an influence on phytochemicals in plants. in the present study, pseudomonas aeruginosa, a plant growth promoting rhizobacterium and paecilomyces lilacinus, an egg parasite of root knot and ...201526373176
antagonistic potential of native trichoderma viride strain against potent tea fungal pathogens in north east india.indigenous strains of trichoderma species isolated from rhizosphere soils of tea gardens of assam, north eastern state of india were assessed for in vitro antagonism against two important tea fungal pathogens namely pestalotia theae and fusarium solani. a potent antagonist against both tea pathogenic fungi, designated as sdrlin1, was selected and identified as trichoderma viride. the strain also showed substantial antifungal activity against five standard phytopathogenic fungi. culture filtrate ...201526361476
phylogenetic diversity of human pathogenic fusarium and emergence of uncommon virulent species.fusarium species cause a broad spectrum of infections. however, little is known about the etiological agents to the species level. we identified fusarium species isolated from clinical specimens including those of high risk patients to better understand the species involved in the pathogenesis.201526348828
rhizopus arrhizus and fusarium solani concomitant infection in an immunocompromised host.neutropenic patients are at risk of the development of hyalohyphomycosis and mucormycosis. correct identification is essential for the initiation of the specific treatment, but concomitant mold infections are rarely reported. we report one unprecedented case of concomitant mucormycosis and fusariosis in a neutropenic patient with acute myeloid leukemia. the patient developed rhino-orbital infection by rhizopus arrhizus and disseminated infection by fusarium solani. the first culture from a sinus ...201626346377
biocontrol of tomato plant diseases caused by fusarium solani using a new isolated aspergillus tubingensis ctm 507 glucose oxidase.the present study focuses on the potential of glucose oxidase (god) as a promising biocontrol agent for fungal plant pathogens. in fact, a new god producing fungus was isolated and identified as an aspergillus tubingensis. god (125 au) has been found to inhibit fusarium solani growth and spore production. indeed, god caused the reduction of spores, the formation of chlamydospores, the induction of mycelial cords and the vacuolization of mycelium. in vivo assays, god acted as a curative treatment ...201526299190
fungal peritonitis due to fusarium solani species complex sequential isolates identified with dna sequencing in a kidney transplant recipient in brazil.fungal peritonitis is a rare serious complication most commonly observed in immunocompromised patients under peritoneal dialysis. nevertheless, this clinical condition is more difficult to treat than bacterial peritonitis. bacterial peritonitis followed by the use of antibiotics is the main risk factor for developing fungal peritonitis. candida spp. are more frequently isolated, and the isolation of filamentous fungi is only occasional. here we describe a case of fusarium solani species complex ...201526296944
structural and functional studies of a fusarium oxysporum cutinase with polyethylene terephthalate modification potential.cutinases are serine hydrolases that degrade cutin, a polyester of fatty acids that is the main component of plant cuticle. these biocatalysts have recently attracted increased biotechnological interest due to their potential to modify and degrade polyethylene terephthalate (pet), as well as other synthetic polymers.201526291558
germin-like protein 2 gene promoter from rice is responsive to fungal pathogens in transgenic potato plants.controlled transgene expression via a promoter is particularly triggered in response to pathogen infiltration. this is significant for eliciting disease-resistant features in crops through genetic engineering. the germins and germin-like proteins (glps) are known to be associated with plant and developmental stages. the 1107-bp oryza sativa root glp2 (osrglp2) gene promoter fused to a β-glucuronidase (gus) reporter gene was transformed into potato plants through an agrobacterium-mediated transfo ...201626277722
a comparative analysis of distribution and conservation of microsatellites in the transcripts of sequenced fusarium species and development of genic-ssr markers for polymorphism analysis.we used an in silico approach to survey and compare microsatellites in transcript sequences of four sequenced members of genus fusarium. g + c content of transcripts was found to be positively correlated with the frequency of ssrs. our analysis revealed that, in all the four transcript sequences studied, the occurrence, relative abundance and density of microsatellites varied and was not influenced by transcript sizes. no correlation between relative abundance and transcript sizes was observed. ...201526269381
new constitutive latex osmotin-like proteins lacking antifungal activity.proteins that share similar primary sequences to the protein originally described in salt-stressed tobacco cells have been named osmotins. so far, only two osmotin-like proteins were purified and characterized of latex fluids. osmotin from carica papaya latex is an inducible protein lacking antifungal activity, whereas the calotropis procera latex osmotin is a constitutive antifungal protein. to get additional insights into this subject, we investigated osmotins in latex fluids of five species. ...201526231325
evaluation of the matrix-assisted laser desorption/ionization time-of-flight mass spectrometry bruker biotyper for identification of penicillium marneffei, paecilomyces species, fusarium solani, rhizopus species, and pseudallescheria boydii.we evaluated the performance of matrix-assisted laser desorption ionization-time of flight mass spectrometry (maldi-tof ms), the maldi bruker biotyper system (microflex lt; bruker daltonik gmbh, bremen, germany), on the identification of 50 isolates of clinically encountered molds, including penicillium marneffei (n = 28), paecilomyces species (n = 12), fusarium solani (n = 6), rhizopus species (n = 3), and pseudallescheria boydii (n = 1). the isolates were identified to species levels by sequen ...201526217315
selection and molecular identification of fungal isolates that produce xylanolytic enzymes.xylan is a heteropolysaccharide and its complete hydrolysis involves a complex set of xylanolytic enzymes. fungal xylanases have been widely used in the holocellulose industry to obtain by-products or for its elimination. the aim of this study was to select and identify filamentous fungi from different ecosystems that produce extracellular xylanases showing biotechnological potential. one hundred three fungal isolates were obtained from orchard, horticultural, and forest ecosystems. the ability ...201526214493
endophytic bacteria from piper tuberculatum jacq.: isolation, molecular characterization, and in vitro screening for the control of fusarium solani f. sp piperis, the causal agent of root rot disease in black pepper (piper nigrum l.).endophytic bacteria have been found to colonize internal tissues in many different plants, where they can have several beneficial effects, including defense against pathogens. in this study, we aimed to identify endophytic bacteria associated with roots of the tropical piperaceae piper tuberculatum, which is known for its resistance to infection by fusarium solani f. sp piperis, the causal agent of black pepper (piper nigrum) root rot disease in the amazon region. based on 16s rrna gene sequence ...201526214435
biotechnological prospecting of foliar endophytic fungi of guaco (mikania glomerata spreng.) with antibacterial and antagonistic activity against phytopathogens.mikania glomerata (spreng.), popularly known as "guaco", is a plant from the asteraceae family that has many therapeutic properties. the use of medicinal plants has been examined in studies on endophytic diversity and bioprospecting; endophytes inhabit the interior of plants without harming them. microorganism-host complex interactions are related to the production of compounds that may confer resistance to pathogens or to production of bioactive compounds or growth regulators. in this study, we ...201526214408
a single-step purification of cauliflower lysozyme and its dual role against bacterial and fungal plant pathogens.a novel lysozyme from cauliflower was purified in a single step, for the first time, using sephadex g100 column chromatography. the purified lysozyme exhibited a homogenized single band in sodium dodecyl sulfate polyacrylamide gel electrophoresis (sds-page), and its molecular mass was calculated to be 22.0 kda. the purified lysozyme showed activity between 30 to 60 °c with 40 °c as the optimum temperature for its maximal activity. although the purified lysozyme was functional at ph ranges betwee ...201526208688
antifungal efficiency of a lipopeptide biosurfactant derived from bacillus subtilis spb1 versus the phytopathogenic fungus, fusarium solani.bacillus subtilis spb1 lipopeptides were evaluated as a natural antifungal agent against fusarium solani infestation. in vitro antifungal assay showed a minimal inhibitory concentration of about 3 mg/ml with a fungicidal mode of action. in fact, treatment of f. solani by spb1 lipopeptides generated excessive lyses of the mycelium and caused polynucleation and destruction of the related spores together with a total inhibition of spore production. furthermore, an inhibition of germination potency ...201526178831
bioprocess development for docosahexaenoic acid from novel fungal isolate of fusarium solani.fungal cultures were isolated from soil samples collected from the western ghats regions of kerala. primary screening of isolated strains were done by sudan black staining method and 15 lipid producing cultures were isolated. the fatty acid profiling of the positive strains were analyzed for docosahexaenoic acid (dha) production. selected oleaginous cultures were grown in submerged culture condition to study the biomass yield and poly unsaturated fatty acid, dha production. the optimization of p ...201526155676
anatomy of a nonhost disease resistance response of pea to fusarium solani: pr gene elicitation via dnase, chitosan and chromatin alterations.of the multiplicity of plant pathogens in nature, only a few are virulent on a given plant species. conversely, plants develop a rapid "nonhost" resistance response to the majority of the pathogens. the anatomy of the nonhost resistance of pea endocarp tissue against a pathogen of bean, fusarium solani f.sp. phaseoli (fsph) and the susceptibility of pea to f. solani f sp. pisi (fspi) has been described cytologically, biochemically and molecular-biologically. cytological changes have been followe ...201526124762
diversity of endophytic fungi in glycine max.endophytic fungi are microorganisms that live within plant tissues without causing disease during part of their life cycle. with the isolation and identification of these fungi, new species are being discovered, and ecological relationships with their hosts have also been studied. in glycine max, limited studies have investigated the isolation and distribution of endophytic fungi throughout leaves and roots. the distribution of these fungi in various plant organs differs in diversity and abundan ...201526111593
fatal fusarium solani species complex infections in elasmobranchs: the first case report for black spotted stingray (taeniura melanopsila) and a literature review.fusarium species are environmental saprophytic fungi. among the many fusarium species, members of the fusarium solani species complex (fssc) are the most prevalent and virulent in causing human and animal infections. in this study, we describe the first case of fatal fssc infection in a black spotted stingray and three concomitant infections in scalloped hammerhead sharks. in the stingray, cutaneous lesions were characterised by ulcers and haemorrhage of the ventral pectoral fin, or 'ray', espec ...201526095191
screen for soil fungi highly resistant to dichloroaniline uncovers mostly fusarium species.arylamines are frequent pollutants in soils. fungi have proven to be efficient in detoxifying these chemicals by acetylating them using arylamine n-acetyl transferase enzymes. here, we selected from natural soils fungi highly resistant to 3,4-dichloroaniline (dca). fusarium species were the most frequently isolated species, especially fusarium solani. the sequenced strain of f. solani contains five nat genes, as did all the dca-resistant isolates. rt-pcr analysis showed that the five genes were ...201526068093
chemical composition, antioxidant and antimicrobial activities of basil (ocimum basilicum) essential oils depends on seasonal variations.chemical composition, antioxidant and antimicrobial activities of the essential oils from aerial parts of basil (ocimum basilicum l.) as affected by four seasonal, namely summer, autumn, winter and spring growing variation were investigated. the hydro-distilled essential oils content ranged from 0.5% to 0.8%, the maximum amounts were observed in winter while minimum in summer. the essential oils consisted of linalool as the most abundant component (56.7-60.6%), followed by epi-α-cadinol (8.6-11. ...200826065762
effects of fusarium solani and f. oxysporum infection on the metabolism of ginsenosides in american ginseng roots.american ginseng (panax quinquefolius l.) is a highly valuable herb widely used for medicinal treatments. its pharmacologically important compounds are the ginsenosides, which are secondary metabolites in american ginseng root. the concentrations of ginsenoside in roots can be changed by fungal infection, but it is unclear what specific root tissues are impacted and whether the change is systemic. in this study, american ginseng roots were inoculated with two fungal pathogens (fusarium solani or ...201526060917
three new cutinases from the yeast arxula adeninivorans that are suitable for biotechnological applications.the genes acut1, acut2, and acut3, encoding cutinases, were selected from the genomic dna of arxula adeninivorans ls3. the alignment of the amino acid sequences of these cutinases with those of other cutinases or cutinase-like enzymes from different fungi showed that they all had a catalytic s-d-h triad with a conserved g-y-s-q-g domain. all three genes were overexpressed in a. adeninivorans using the strong constitutive tef1 promoter. recombinant 6× his (6h)-tagged cutinase 1 protein (p) from a ...201526048925
production of 4-ipomeanol, an anticancer agent from the root tubers and rhizogenic callus of ipomoea batatas lam.: a comparative study.a comparative study was done on the production of 4-ipomeanol from root tubers of ipomoea batatas and its rhizogenic callus. best callusing response was obtained on ms medium supplemented with 11 μm naa (α-naphthaleneacetic acid) and 1 μm kin (kinetin). effect of various elicitors (fusarium solani, chitin and chitosan) on the production of 4-ipomeanol was studied. methanol extract of the samples were purified by column chromatography and detected using tlc. identification of 4-ipomeanol was conf ...201526040027
broad-spectrum antimicrobial/antifouling soft material coatings using poly(ethylenimine) as a tailorable scaffold.microbial colonization and biofilm formation is the leading cause of contact lens-related keratitis. treatment of the condition remains a challenge because of the need for prolonged therapeutic course and high doses of antimicrobial agents especially for biofilm eradication. the development of strategies to prepare nonfouling contact lens surfaces is a more practical way to ensure users' safety and relieve the excessive public healthcare burden. in this study, we report a series of polymers that ...201526039032
sertraline enhances the activity of antimicrobial agents against pathogens of clinical relevance.serotonin reuptake inhibitors were recently reported to possess antimicrobial potentials, potentiate activity of several antibiotics, reverse multidrug resistant phenotypes of bacteria and make them susceptible to previously resistant drugs. we investigated antimicrobial potentials of sertraline (sr) against atcc strains, clinical isolates of staphylococcus aureus, escherichia coli and pseudomonas aeruginosa alone and in-combination with seven antibiotics. antifungal activity was investigated ag ...201526029671
antimicrobial, antitumor and brine shrimp lethality assay of ranunculus arvensis l. extracts.to investigate the antitumor activity, brine shrimp lethality assay, antibacterial and antifungal activity of methanol extract (me), water extract (we), acetone extract (ae), chloroform extract (ce), methanol-water extract (mwe), methanol-acetone extract (mae), methanol-chloroform extract (mce) of ranunculus arvensis (l.). antitumor activity was evaluated with agrobacterium tumefaciens (at10) induced potato disc assay. cytotoxicity was evaluated with brine shrimp lethality assay. antibacterial a ...201526004705
keratitis caused by moulds in santa lucía ophthalmology hospital in buenos aires, argentina.mycotic keratitis by moulds (mkm) is an important cause for corneal blindness and usually carries an unfavorable prognosis.201625999088
emergence of fusarioses in a university hospital in turkey during a 20-year period.fusarium species have started appearing increasingly as the main cause of infections, particularly in immunocompromised patients. in this study, we aimed to present the first epidemiological data from turkey, analyze fusariosis cases that have been monitored in a university hospital during the past 20 years, identify the responsible fusarium species, and determine antifungal susceptibilities. a total of 47 cases of fusariosis was included in the study. fusarium isolates were identified by multil ...201525994844
molecular mechanisms underlying the close association between soil burkholderia and fungi.bacterial species belonging to the genus burkholderia have been repeatedly reported to be associated with fungi but the extent and specificity of these associations in soils remain undetermined. to assess whether associations between burkholderia and fungi are widespread in soils, we performed a co-occurrence analysis in an intercontinental soil sample collection. this revealed that burkholderia significantly co-occurred with a wide range of fungi. to analyse the molecular basis of the interacti ...201525989372
inhibition of hmg-coa reductase by mfs, a purified extract from the fermentation of marine fungus fusarium solani fg319, and optimization of mfs production using response surface methodology.the present study was designed to isolate and characterize a purified extract from fusarium solani fg319, termed mfs (metabolite of fusarium solani fg319) that showed anti-atherosclerosis activity by inhibiting 3-hydroxy-3-methylglutaryl coenzyme a (hmg-coa) reductase. response surface methodology (rsm) was employed to achieve an improved yield from the fermentation medium. the inhibiting effect of the isolate, mfs, on hmg-coa reductase was greater than that of the positive control, lovastatin. ...201525986283
the use of response surface methodology as a statistical tool for media optimization in lipase production from the dairy effluent isolate fusarium solani.the optimization of extracellular lipase production by fusarium isolani strain skwf7 isolated from dairy wastewater was carried out in this study. initially, the physicochemical factors significantly influencing enzyme production were studied by varying one-factor-at-a-time (ofat). a mesophilic temperature of 40°c, alkaline ph of 8, and incubation period of 72 hours were found to be the optimal conditions for lipase production. among the media components, the disaccharide sucrose acted as the be ...201325969775
hexacyclopeptides secreted by an endophytic fungus fusarium solani n06 act as crosstalk molecules in narcissus tazetta.the basis of chemical crosstalk in plants and associated endophytes lies in certain so-called communication molecules that are responsible for plant-microbe and microbe-microbe interactions. consequently, elucidating the factors that affect the nature, distribution, and amount of these molecules and how they impact the interaction among endophytes and associated organisms is essential to understand the true potential of endophytes. in the present study, we report the discovery of nine hexacyclop ...201525958995
[fungicidal properties of the nanosystems of silkworm (bombyx mor) chitosan with copper ions].the fungicidal activity of some chitosan samples and of its nanostructured systems with copper was studied. the samples of bombyx mori chitosan and its nanostructured systems with copper were found to inhibit growth and development of phytopathogenic fungi fusarium solani 169 and verticillium dahlie 57. the growth inhibition zone was of considerable size (22 to 60 mm).201525941714
disseminated fusariosis and hematologic malignancies, a still devastating association. report of three new cases.fungi of the genus fusarium are primarily plant pathogens and saprobes that produce disseminated infections in immunologically deficient humans. after aspergillosis, disseminated fusariosis is the second most common cause of invasive infection by filamentous fungi in patients with hematologic malignancies or those undergoing transplants of hematopoietic progenitors.201525936697
filamentous fungal endophthalmitis: results of combination therapy with intravitreal amphotericin b and voriconazole.to report outcomes of exogenous fungal endophthalmitis treated with combination of intravitreal antifungal agents.201525926714
antibacterial, antifungal, and insecticidal potentials of oxalis corniculata and its isolated compounds.oxalis corniculata is a common medicinal plant widely used against numerous infectious diseases. the agrochemical potential of methanolic extract, n-hexane, chloroform, ethyl acetate, and n-butanol fractions were assessed to measure the antibacterial, antifungal, and insecticidal activities of the plant. the crude, chloroform, and n-butanol soluble fractions showed excellent activities against escherichia coli, shigella dysenteriae, salmonella typhi, and bacillus subtilis but have no activity ag ...201525873973
inhibitory effects of antimicrobial agents against fusarium species.we investigated the inhibitory effects of antibacterial, biocidal, and antifungal agents against fusarium spp. seven fusarium spp: four f. falciforme (fusarium solani species complex), one fusarium spp, one fusarium spp. (fusarium incarnatum-equiseti species complex), and one f. napiforme (gibberella fujikuroi species complex), isolated from eyes with fungal keratitis were used in this study. their susceptibility to antibacterial agents: flomoxef, imipenem, gatifloxacin, levofloxacin, moxifloxac ...201525841054
[tinea pedis due to fusarium solani in dakar].a patient presented with intertrigo at the second, third and fourth interdigitals spaces lasting for four years in which fusarium solani was highlighted. the search for contributing factors revealed a concept of foot washing with water at least five times a day for ablutions, associated with wearing closed shoes all day and the absence of immunosuppression and diabetes. the diagnosis of fusarium was made on the basis of direct examination and culture. combined treatment with griseofulvin oral an ...201525835156
isolation, purification, and characterization of a stable defensin-like antifungal peptide from trigonella foenum-graecum (fenugreek) seeds.a novel defensin-like antifungal peptide (tf-afp) with molecular mass of 10.3 kda was isolated from seeds of trigonella foenum-graecum (fenugreek) by ammonium sulfate precipitation, cation-exchange, gel-filtration, hydrophobic chromatography, and rp-hplc. mass spectroscopic analysis revealed the intact mass of the purified antifungal peptide as 10321.5 da and high similarity to plant defensins and other antifungal proteins in database search. 2d-page showed pi value to be 8.8 and absence of isof ...201525761687
candida and fusarium species known as opportunistic human pathogens from customer-accessible parts of residential washing machines.energy constraints have altered consumer practice regarding the use of household washing machines. washing machines were developed that use lower washing temperatures, smaller amounts of water and biodegradable detergents. these conditions may favour the enrichment of opportunistic human pathogenic fungi. we focused on the isolation of fungi from two user-accessible parts of washing machines that often contain microbial biofilms: drawers for detergents and rubber door seals. out of 70 residentia ...201525749362
design, synthesis and in vitro bactericidal/fungicidal screening of some vanadyl(iv)complexes with mono- and di-substituted ons donor triazoles.a new series of anti-bacterial and anti-fungal mono- and di-substituted triazoles (l(1))-(l(6)) have been synthesized and characterized on the basis of their physical, spectral and analytical data. the ligands (l(1))-(l(6)) on reaction with vanadyl(iv) sulphate led to the formation of vanadyl(iv) metal complexes (1)-(4). the structure of the complexes has been established on the basis of their physical, spectral and elemental analyses data. the synthesized ligands and their vanadyl(iv) complexes ...201525716124
coculnol, a new penicillic acid produced by a coculture of fusarium solani fki-6853 and talaromyces sp. fka-65. 201525712393
isolation and characterization of two mitoviruses and a putative alphapartitivirus from fusarium spp.the filamentous fungus fusarium spp. includes several important plant pathogens. we attempted to reveal presence of double-stranded (ds) rnas in the genus. thirty-seven fusarium spp. at the maff collection were analyzed. in the strains of fusarium coeruleum, fusarium globosum and fusarium solani f. sp. pisi, single dsrna bands were detected. the strains of f. coeruleum and f. solani f. sp. pisi cause potato dry rot and mulberry twig blight, respectively. sequence analyses revealed that dsrnas in ...201525687123
fusarium diversity in soil using a specific molecular approach and a cultural approach.fusarium species are ubiquitous in soil. they cause plant and human diseases and can produce mycotoxins. surveys of fusarium species diversity in environmental samples usually rely on laborious culture-based methods. in the present study, we have developed a molecular method to analyze fusarium diversity directly from soil dna. we designed primers targeting the translation elongation factor 1-alpha (ef-1α) gene and demonstrated their specificity toward fusarium using a large collection of fungi. ...201525655778
morphological and comparative genomic analyses of pathogenic and non-pathogenic fusarium solani isolated from dalbergia sissoo.sissoo or shisham (dalbergia sissoo roxb.) is one of the finest wood of south asia. fusarium solani is a causal organism of sissoo wilt, decline, or dieback. it is also a potential causal organism associated with other valuable tree species. thirty-eight fusarium isolates including 24 f. solani and 14 fusarium sp., were obtained in 2005 from different geographical locations in india. all 38 (18 pathogenic and 20 non-pathogenic) isolates were characterized for genomic analysis, growth behaviour, ...201525605046
enhanced camptothecin production by ethanol addition in the suspension culture of the endophyte, fusarium solani.ethanolic extract of a non-camptothecin producing plant, catharanthus roseus when added in the suspension culture of the endophyte fusarium solani known to produce camptothecin, resulted in enhanced production of camptothecin by 10.6-fold in comparison to that in control (2.8 μg/l). interestingly, addition of pure ethanol (up to 5% v/v) in the suspension culture of f. solani resulted in maximum enhancement in camptothecin production (up to 15.5-fold) from that obtained in control. in the presenc ...201525603728
in silico analysis and prioritization of drug targets in fusarium solani.mycotic keratitis has emerged as a major ophthalmic problem and a leading cause of blindness, since its recognition in 1879. filamentous fungi are major causative of mycotic keratitis. in india, the main etiological organism responsible for mycotic keratitis is aspergillus species followed by fusarium species. in south india, fusarium based keratitis scales up to 43%. nearly one-third of mycotic keratitis treatment results in failure, as fungal infections are highly resistant to antibiotic thera ...201525555413
a substrate fed-batch biphasic catalysis process for the production of natural crosslinking agent genipin with fusarium solani accc 36223.the natural crosslinking agent genipin has been applied widely in biomedicines and foods nowadays. because of the special hemiacetal ring structure in its molecule, it can only be prepared by hydrolysis of geniposide according to biocatalysis. in this research, strategies including aqueous-organic biphasic catalysis and substrate fed-batch mode were adopted to improve the biocatalysis process of genipin. a 10 l ethyl acetate-aqueous biphasic system with geniposide fed-batch led to a satisfying g ...201525537719
spectrum of fusarium infections in tropical dermatology evidenced by multilocus sequencing typing diagnostics.fusarium species are emerging causative agents of superficial, cutaneous and systemic human infections. in a study of the prevalence and genetic diversity of 464 fungal isolates from a dermatological ward in thailand, 44 strains (9.5%) proved to belong to the genus fusarium. species identification was based on sequencing a portion of translation elongation factor 1-alpha (tef1-α), rdna internal transcribed spacer and rna-dependent polymerase subunit ii (rpb2). our results revealed that 37 isolat ...201525530264
the galactolipase activity of fusarium solani (phospho)lipase.the purified (phospho)lipase of fusarium solani (fsl), was known to be active on both triglycerides and phospholipids. this study aimed at assessing the potential of this enzyme in hydrolyzing galactolipids. fsl was found to hydrolyze at high rates of synthetic medium chains monogalactosyldiacylglycerol (4658±146u/mg on dic8-mgdg) and digalactosyldiacylglycerol (3785±83u/mg on dic8-dgdg) and natural long chain monogalactosyldiacylglycerol extracted from leek leaves (991±85u/mg). it is the microb ...201525529980
development of a selective medium for the fungal pathogen cylindrocarpon destructans using radicicol.the soil-borne ascomycete fungus cylindrocarpon destructans causes ginseng root rot disease and produces various secondary metabolites such as brefeldin a and radicicol. the slow growth of this fungus compared with other plant pathogenic and saprophytic fungi in soil disturbs isolation of this fungus from soil and infected ginseng. in this study, we developed a selective medium for c. destructans using radicicol produced by this fungus. supplementing 50 mg/l of radicicol to medium inhibited the ...201425506308
fungal isolation from disinfectant solutions of contact lens storage cases among asymptomatic users.to investigate the incidence of fungal contamination of store disinfectant solutions contained in contact lens storage cases belonging to asymptomatic contact lens users.201525503912
Displaying items 101 - 200 of 1378