Publications

reverse transcription polymerase chain reaction (rt-pcr) used for the detection of taura syndrome virus (tsv) in experimentally infected shrimp.	taura syndrome virus (tsv) has adversely affected the shrimp culture industries of the americas. first recognized in 1992, this viral agent has spread throughout the shrimp growing regions of south and central america to become established in north america in the short span of 5 yr. diagnostic methods for tsv include histopathology, bioassay using susceptible penaeus vannamei as the indicator species and in situ hybridization with tsv specific complimentary dna (cdna) gene probes. an additional ...	1998	9828404
taura syndrome of penaeid shrimp: cloning of viral genome fragments and development of specific gene probes.	the ssrna genome extracted from purified taura syndrome virus (tsv) was transcribed into double-stranded, blunt-ended cdna and was used to construct cdna libraries either in puc 18 or in pbluescript ii ks-vectors. twelve recombinant plasmids chosen after screening of the libraries were subjected to restriction enzyme digestions for determination of size inserts and restriction maps. two of them, pp15 and pq1, were selected for probe construction. the inserts, 1500 and 1300 base pairs (bp) respec ...	1998	9653455
a new rna-friendly fixative for the preservation of penaeid shrimp samples for virological detection using cdna genomic probes.	in situ hybridization analysis of shrimp histological sections, utilizing taura syndrome virus (tsv) specific cdna probes, is the most sensitive diagnostic technique presently available for the detection of this penaeid shrimp viral disease. however, false negative genomic probe results are obtained frequently from samples of pacific white shrimp, penaeus vannamei, that have been preserved with davidson's afa (acetic acid, formaldehyde, alcohol) fixative and that, otherwise, demonstrate pathogno ...	1997	9255734
susceptibility to taura syndrome virus of some penaeid shrimp species native to the gulf of mexico and the southeastern united states	experimental studies demonstrated that penaeus setiferus, but not penaeus aztecus or penaeus duorarum, could be killed by taura syndrome virus (tsv). however, specimens of p. setiferus that survived infection and both p. aztecus and p. duorarum at least 79 days postexposure that did not demonstrate gross signs of infection were shown to harbor virus by bioassay using penaeus vannamei, a highly susceptible target host. consequently, all three of those penaeids native to the southeast united state ...	1997	9056467