Publications

TitleAbstractYear
Filter
PMID(sorted descending)
Filter
anti-complement activity in the saliva of phlebotomine sand flies and other haematophagous insects.the saliva of haematophagous insects has a series of pharmacological activities which may favour blood feeding. in the present study, an inhibitory effect on the complement system was observed in salivary extracts obtained from the phlebotomine sand flies lutzomyia longipalpis and lu. migonei. saliva from lu. longipalpis was capable of inhibiting both the classical and alternative pathways, while that from lu. migonei acted only on the former. other haematophagous insect species were screened fo ...200312885192
blood-feeding performance of nymphs and adults of triatoma brasiliensis on human hosts.the blood-feeding behaviour of nymphs and adults of triatoma brasiliensis fed on the forearm of human volunteers was studied by electronic monitoring of the cibarial pump. parameters of total contact time (tt), initial weight (iw), weight gain (wg), ingestion rate (ir), pump frequency (f), quantity of liquid ingested per cibarial pump stroke (qlc) and non-ingestive time (nit) (cumulative probing time and pumping interruptions during blood feeding) were measured. protein profile (sds-page) and qu ...200312875930
ecologic niche modeling and differentiation of populations of triatoma brasiliensis neiva, 1911, the most important chagas' disease vector in northeastern brazil (hemiptera, reduviidae, triatominae).ecologic niche modeling has allowed numerous advances in understanding the geographic ecology of species, including distributional predictions, distributional change and invasion, and assessment of ecologic differences. we used this tool to characterize ecologic differentiation of triatoma brasiliensis populations, the most important chagas' disease vector in northeastern brazil. the species' ecologic niche was modeled based on data from the fundação nacional de saúde of brazil (1997-1999) with ...200212479554
molecular cloning and sequencing of salivary gland-specific cdnas of the blood-sucking bug triatoma brasiliensis (hemiptera: reduviidae).haematophagous insects produce pharmacological substances in their saliva to counteract vertebrate host haemostasis events such as coagulation, vasoconstriction and platelet aggregation. to investigate the bioactive salivary molecules of the triatomine bug triatoma brasiliensis, we produced subtraction-enriched cdnas of salivary-gland specific genes using suppression subtractive hybridization. six full-length differentially expressed cdnas (tb113, tb125, tb152, tb169, tb180 and tb198) were selec ...200212421416
fungal flora of the digestive tract of 5 species of triatomines vectors of trypanosoma cruzi, chagas 1909.a study of the mycobiota in the digestive tract of 5 important species of triatomines, triatoma brasiliensis, t infestans, t. sordida, t. pseudomaculata and t. vitticeps, was made. the digestive tracts of 164 adults and 535 nymphs of those triatomines were studied and 393 fungal strains were isolated. the genera with the greatest number of species were penicillium (19 species), aspergillus (17 species) and acremonium (5 species) and the most frequent species, in decreasing order, were penicilliu ...200111502063
genetic variability of triatoma brasiliensis (hemiptera: reduviidae) populations.isoenzymes, random amplified polymorphic dna (rapd), and morphometry were used to compare genetic variability within and between three populations of triatoma brasiliensis neiva, 1911 from northeast brazil. the isoenzyme profiles were identical for the three populations, whereas the complex rapd patterns allowed clear discrimination between the three. morphometric analysis, using characters of the head capsule, also showed discrimination between the three populations but only in comparisons of m ...200011126543
[focal and total residual insecticide spraying to control triatoma brasiliensis and triatoma pseudomaculata in northeast brazil].to efficiently control the triatomines triatoma brasiliensis and triatoma pseudomaculata, a field trial was performed to compare conventional versus focal spraying of deltamethrin 5% sc at 25 mg a. i./m2 and the slow-release organophosphate malathion 8.3% sr at 2g a. i./m2. the assay took place in the county of boa viagem, ceará state, with 1541 households, randomly separated into 4 groups. two of them received focal spraying: pt, treated with deltamethrin indoors and in the peridomicile, and pl ...200011119328
[biological potential of triatoma brasiliensis].biological and physiological parameters of triatoma brasiliensis, triatoma infestans, and triatoma pseudomaculata were studied and compared. t. brasiliensis, reared on mice, showed a faster evolutionary cycle from first stage to adult and higher fecundity, when compared to the other species. t. infestans showed the fastest blood intake in both hosts tested, followed by t. brasiliensis and t. pseudomaculata. clotting tests using salivary gland extracts of t. brasiliensis presented intermediary va ...200011119327
[use of random amplified polymorphic dna (rapd) in the populational study of triatoma brasiliensis neiva, 1911].we evaluated the genetic variability of triatoma brasiliensis, the main vector of chagas disease in northeast brazil, using specimens from three populations. regions of genomic dna were amplified by rapd (random amplified polimorphic dna), using two primers. the products were visualized after polyacrylamide gel electrophoresis followed by silver staining. a dendrogram constructed through the dice similarity coefficient allowed for separation of the tested specimens into three distinct groups. th ...200011119326
[distribution and characterization of different populations of triatoma brasiliensis neiva, 1911 (hemiptera, reduviidae, tritominae)].triatoma brasiliensis neiva, 1911 is now considered the most important chagas disease vector in the semiarid zones of northeastern brazil. four distinct populations of t. brasiliensis have been identified by multidisciplinary studies: brasiliensis (caicó, rn), melânica (espinosa, mg), macromelasoma (petrolina, pe), and juazeiro (juazeiro, ba). by scanning electron microscopy of egg exochorion, each population displayed a distinct ornamentation pattern. the brasiliensis, macromelasoma, and juazei ...200011119325
chromosome homogeneity in populations of triatoma brasiliensis neiva 1911 (hemiptera - reduviidae - triatominae).triatoma brasiliensis is the most important vector of chagas disease in the semiarid zone of the northeast of brazil. several authors have reported the occurrence of four chromatic patterns with morphological, ecological, and genetic differences. in order to determine the existence of cytogenetic differentiation between these chromatic forms, we analyzed their karyotypes and the chromosome behavior during the male meiotic process. triatoma brasiliensis shows distinct and specific chromosome char ...200011119323
[peridomiciliary changes and implications for triatoma brasiliensis control].a total of 9,906 annexes from 1,541 rural dwellings of boa viagem county, ceará, brazil, infested by triatoma brasiliensis and triatoma pseudomaculata were investigated and immediately sprayed with pyrethroid insecticide, followed by revisions at 6, 12, and 18 months. the initial infestation rate of annexes was 4.0%, with predominant infestation in animal shelters (7.0%). mean insect density was low, regardless of triatomine species or type of annex. a progressive decrease in the number of initi ...200011119322
[microclimatic properties of the triatoma brasiliensis habitat].vector-borne transmission of chagas disease in northeast brazil is basically by triatoma brasiliensis. it is thus crucial to determine this species' microclimatic preferences as limiting factors for its distribution and ability to infest domestic environments. we analyze the microclimatic properties of the shelters in which these insects are found in wild, domestic, and peridomiciliary environments in the state of ceará, at brazil. we measure temperature and relative humidity (rh) every 15 minut ...200011119321
[operational aspects of triatoma brasiliensis control].vector control strategies against indigenous species is not easy, due to their capacity to reinvade treated premises from sylvatic ecotopes. between august 1996 and december 1997 we conducted a study on reinfestation of houses after spraying in a county in the state of ceará. of 277 houses examined, 113 (40.8%) were infested (21.7% intradomiciliary and 35.4% peridomiciliary). of the 433 triatominae collected, 207 were triatoma brasiliensis (49% of which intradomiciliary, with a mean of 1.8 insec ...200011119320
[general situation and perspectives of chagas disease in northeastern region, brazil].primary and secondary data show the importance and distribution of human chagas disease (hcd) in northeast brazil. among the 27 detected vector species, triatoma infestans, panstrongylus megistus, triatoma brasiliensis and triatoma pseudomaculata are epidemiologically important. major medical impact is attributed to t. infestans and p. megistus, the most domiciliated and vulnerable species, while the other two are native and more difficult to control. regional differences in transmission and med ...200011119317
performance of yeast-baited traps with triatoma sordida, triatoma brasiliensis, triatoma pseudomaculata, and panstrongylus megistus in laboratory assays.the effectiveness of a trap for triatomines baited with yeast cultures has been previously demonstrated for triatoma infestans in laboratory assays. we report here results from laboratory assays testing yeast traps for triatoma sordida, triatoma brasiliensis, triatoma pseudomaculata, and panstrongylus megistus. all assays were conducted in an open experimental arena 100 cm x 100 cm, with two traps placed at opposite sides of the arena. one of the traps contained a yeast culture, and the other tr ...200010949899
comparison of feeding behaviour of triatoma infestans, triatoma brasiliensis and triatoma pseudomaculata in different hosts by electronic monitoring of the cibarial pump.feeding behaviour of triatoma infestans, t. brasiliensis and t. pseudomaculata on pigeons and mice was compared by electronic monitoring of the cibarial pump. the methodology developed permits the study detailed of triatomine feeding behaviour using an artificial feeder as well as on live hosts. t. infestans was the species that fed most rapidly on the two hosts tested (28.03+/-1.6 mg/min for pigeon and 21.33+/-1.7 mg/min for mouse), followed by t. brasiliensis (17.09+/-1.4 mg/min and 13.1+/-1.5 ...200010817838
population dynamics and feeding behavior of triatoma brasiliensis and triatoma pseudomaculata, main vectors of chagas disease in northeastern brazil.biological parameters of triatoma brasiliensis and t. pseudomaculata that could influence the epidemiological importance of these insects as vectors of trypanosoma cruzi were compared. the parameters studied were incubation period, interval between hatching or moulting and first feeding, number of blood meals, development time, mortality, net reproductive rate, instantaneous daily reproductive rate, time-lapse before starting feeding, duration of feeding, blood ingestion capacity, occurrence of ...200010733731
survey for trypanosoma cruzi infection in a municipality in northeast brazil.an epidemiological survey of trypanosoma cruzi infection was carried out in bodocó, located in the western part of the state of pernambuco, brazil. two hundred and forty-one individuals were parasitologically and immunologically screened. although hemoculture did not reveal the presence of parasites in the blood, the sera of 5 individuals were scored as positive by the indirect fluorescence antibody test and the enzyme-linked immunosorbent assay. seropositivity in individuals above and below the ...199910733161
the synanthropic process of chagas disease vectors in brazil, with special attention to triatoma brasiliensis neiva, 1911 (hemiptera, reduviidae, triatominae) population, genetical, ecological, and epidemiological aspects. 199910677724
beauveria bassiana (hyphomycetes) as a possible agent for biological control of chagas disease vectors.beauveria bassiana (balsamo) vuillemin (isolate cg306) was tested on 3rd instars of 9 triatoma spp., 4 rhodnius spp., 2 panstrongylus spp. and dipetalogaster maxima (uhler) at 25 degrees c and 50% rh. quantitative sporulation of the fungus on cadavers was studied at 25 degrees c and 97% rh. mortality, estimates of survival time, and conidial production on cadavers differed significantly among the genera and species tested. panstrongylus herreri wygodzinsky, dipetalogaster maxima, triatoma pictur ...19989835689
ecotopes, natural infection and trophic resources of triatoma brasiliensis (hemiptera, reduviidae, triatominae).triatoma brasiliensis is considered as one of the most important chagas disease vectors in the northeastern brazil. this species presents chromatic variations which led to descriptions of subspecies, synonymized by lent and wygodzinsky (1979). in order to broaden bionomic knowledge of these distinct colour patterns of t. brasiliensis, captures were performed at different sites, where the chromatic patterns were described: caicó, rio grande do norte (t. brasiliensis brasiliensis neiva, 1911), it ...19989698835
[triatominae species]this paper provides a checklist of 15 known species of the triatominae subfamily in maranhão state. on the coastal são luís island there are a total of 10 species associated with sylvatic areas (eratyrus mucronatus, psammolestes tertius, panstrongylus diasi) and periurban and urban areas (panstrongylus geniculatus, panstrongylus lignarius, rhodnius nasutus, rhodnius neglectus, rhodnius pictipes, rhodnius robustus and triatoma rubrofasciata). the last was found only on são luís island, while the ...19989592224
isoenzymes detect variation in populations of triatoma brasiliensis (hemiptera: reduviidae: triatominae).triatoma brasiliensis is one of the most important vectors of chagas disease in the semiarid zone of the northeast of brazil. intraspecific morphological and behavioural variation has been reported for different populations. results for four distinct populations using eight isoenzymes are reported here. the literature describes three subspecies: t. brasiliensis brasiliensis neiva, 1911; t. brasiliensis melanica neiva & lent, 1941 and t. brasiliensis macromelasoma galvão, 1956. these subspecies d ...19979361737
reproductive isolation between triatoma brasiliensis neiva, 1911 and triatoma petrochii pinto & barreto, 1925 (hemiptera reduviidae). 19714945961
[resistence of triatoma brasiliensis to fasting]. 20154596930
[epidemiology of chagas disease in the municipalities of castelo do piaui and pedro ii, state of piaui, brazil].triatoma brasiliensis, triatoma pseudomaculata and rhodnius nasutus were captured in artificial and natural echotopes in ten localities situated in rural areas of castelo do piauí and pedro ii, state of piauí, brazil. nymphal instars predominated and birds were the main blood meal source for triatomines. t. brasiliensis was the only triatomine captured inside the houses searched, harbouring flagellates morphologically and biologically indistinguishable from trypanosoma (schizotrypanum) cruzi. sp ...19892517851
possible oral transmission of acute chagas' disease in brazil.in october 1986, 7 to 22 days after a meeting at a farm in paraíba state, 26 individuals presented with a febrile illness associated with bilateral eyelid and lower limb edema, mild hepatosplenomegaly, lymphadenopathy and, occasionally a skin rash. a 11-year-old boy exhibited atrial premature complexes and a 74-year-old patient developed acute heart failure. in two patients hospitalized in são paulo city, acute chagas' disease was diagnosed by the demonstration of circulating trypanosoma cruzi. ...19911844961
lethality of triatomines (hemiptera: reduviidae), vectors of chagas' disease, feeding on blood baits containing synthetic insecticides, under laboratory conditions.a laboratory study was conducted to test the toxicity of synthetic insecticides added to defibrinated sheep blood kept at room temperature and offered as food to the following triatomine species: triatoma infestans, panstrongylus megistus, triatoma vitticeps, triatoma pseudomaculata, triatoma brasiliensis and rhodnius prolixus. the insecticides used, at a concentration of 1 g/l, were: hch, ddt, malathion and trichlorfon, and the lethalithy observed at the end of a 7-day period varied according t ...19911726952
influence of light conditions on adult mortality and egg-laying of triatoma brasiliensis neiva, 1911 (hemiptera: reduviidae: triatominae).we compared, for triatoma brasiliensis, the egg-laying process and the mortality of adults under conditions of almost permanent darkness and with normal laboratory luminosity. mortality did not differ between groups. the egg-laying per vial and per female was significantly greater in the group of normal luminosity. we consider that it is not recommendable to keep the adults of this species under complete darkness. other biological aspects should be analysed in relation to luminosity.19921340536
[the epidemiology of chagas' disease in a rural area of the city of teresina, piauí, brazil].in the rural areas of teresina, 129 triatomines were captured distributed in (a) artificial ecotopes; a house with one triatoma brasiliensis, one panstrongylus geniculatus, rhodnius pictipes, and one rhodnius prolixus and in a uninhabited chicken house (7 rhodnius nasutus). (b) natural ecotopes; pahus orbignya martiana (41 rhodnius neglectus, 33 rhodnius prolixus and 41 rhodnius nasutus) and copernicia cerifera (3 rhodnius neglectus). the 22.6% of captured triatomines were infected by flagellate ...19921308066
feeding behavior of different species of chagas' disease vectors stimulated with blood sources at different temperatures.1. the feeding behavior of six triatomid species toward latex condoms filled with blood at 26 +/- 1 and 36 +/- 1 degrees c was observed for 4 h. 2. the species studied were triatoma infestans, triatoma brasiliensis, triatoma vitticeps, triatoma pseudomaculata, rhodnius prolixus and panstrongylus megistus, and 34 to 348 insects were studied in each group (average, 190). all the life stages of each species were used. 3. although most insects (80%, average for 6 species) preferred the warmer blood, ...19921304939
[biology of triatoma braziliensis. ii. observations on its autogeny]. 1975766149
lipoprotein lipase in the fat body of triatoma brasiliensis.lipoprotein lipase activity was detected and characterized in the fat body of triatoma brasiliensis one of the vector of chagas' disease. the enzyme activity was close to a free fatty acid production of 47 mumol ffa mg protein-1-h-1 when assayed in the complete system. 2. the enzyme presented maximum activity at ph 8.5. it appeared to be activated by heparin (2 u/ml) and to require a cofactor (rat serum lipoproteins) when assayed with simple triglyceride emulsions. the requirement for a ffa acc ...197660918
Displaying items 101 - 134 of 134